Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008631

Physical Location & Seq

Genomic Coordinates Forward (+)

20585349 .. 20587761

Gene Structure 2413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G50580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 957 bp

ATGGACGGAGATGAGCTCACCGAGCAGGAAACTGCTCTTTATGACCGTCAAATTAGGGTATGGGGAGCTGGTGCTCAAAGAAGATTGAGTAAATCTCATGTTTTGGTATCTGGAATTAAGGGCACTGTTGCTGAGTTTTGTAAGAACATTGTGTTGGCAGGAGTAGGTAGTGTGACTCTGTTGGATGATCGATTGGTTACAACGGAAGTCTTCAACGCAAACTTCTTGATTCTTCCTGATGAAAATGCTTATGTAGGCAAAACCGTAGCTGAGATTTGTTGTGATTCACTTAAGGATTTTAACCCTATGGTTCATGTTTCAATAGAGAAAGGTGATTTGTCAACACTTGGTGTTGATTTTTTCGAGAAGTTTGATGTTGTGGTTATCGGCTACAGTTCTCGTGCTACTAAGAAAGCAGTTAATGAAAAGTGTAGGAACCTAGCAAAAGATGTAGCGTTCTATACAGTTGATTGTAGAGGCTCATGTGGCGAAATATTTGTCGACCTTCAGAATTATAAGTACACAAAGCTTGATGAAACAGTGGAATGTGAACTAACATTTCCAAGTTTTGAGGAGGCAGTTTCAGTACCGTGGAAACCGATGCCAAGGAGAACAGCAAAGCTCTACTTTGCAATGAGAGTGATAGAACTATTTGAGGAAACTGAAGGACGTAAACCTGGGGAATGTTCGCTGTCTGATCTCCCAAGAGTTTTGAAACTCAAAAAGGAACTATGTGAAGGAAATTCGGTCAGCGAGAATCATATTCCAGATATCCTCTTGGAGAGACTCGTATCTAATAACACAGAATTCCCACCGGCTTGTGCCATCATTGGAGGGATTCTAGGACAGGAGGTGATAAAAGTTATATCAGGCAAAGGAGAGCCGTTGAAGAACTTCTTCTACTTTGATGCAGAGGATGGGAAAGGTGTAATCGAGGACCTATCCCACAAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008641 GO:0009987 GO:0016874 GO:0016877 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0019948 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

ThiF

Protein Analysis

318

Amino Acids

35.4

Weight (kDa)

5.02

Isoelectric Point (pI)

37.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	14 - 304	1e-24	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008631)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24940 AT5G50580 AT5G50580 AT5G50680 AT5G50680
fragaria_vesca	FvH4_5g08600 FvH4_5g08600 FvH4_5g08600 FvH4_5g08600
malus_domestica	MD06G1138700.v1.1 MD14G1154700.v1.1
prunus_persica	Prupe.5G148900_v2.0.a1 Prupe.5G148900_v2.0.a1
pyrus_communis	pycom06g12980 pycom14g12870
rosa_chinensis	RchiOBHm_Chr7g0189801
rosa_laevigata	RLG00000004589
rosa_multiflora	Rmu_sc0014337.1_g000003
rosa_roxburghii	Rroxscaffold_3G00265150
rosa_rugosa	Rorug06G0500000
rosa_samantha	Rh7AG105100 Rh7CG109700
rosa_wichuraiana	Rw7G009100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	516
AccI	GTMKAC	1 cut(s)	501
AcsI	RAATTY	2 cut(s)	742, 806
AcuI	CTGAAG	2 cut(s)	491, 684
AdeI	CACNNNGTG	1 cut(s)	350
AfaI	GTAC	2 cut(s)	521, 588
AflII	CTTAAG	1 cut(s)	290
AgsI	TTSAA	4 cut(s)	214, 321, 715, 889
AjnI	CCWGG	1 cut(s)	676
AjuI	GAANNNNNNNTTGG	2 cut(s)	137, 169
AluBI	AGCT	6 cut(s)	16, 68, 269, 529, 622, 952
AluI	AGCT	6 cut(s)	16, 68, 269, 529, 622, 952
Alw21I	GWGCWC	2 cut(s)	18, 76
Alw26I	GTCTC	1 cut(s)	778
ApoI	RAATTY	2 cut(s)	742, 806
ArsI	GACNNNNNNTTYG	2 cut(s)	483, 515
Asp700I	GAANNNNTTC	2 cut(s)	893, 896
AspS9I	GGNCC	1 cut(s)	937
AsuHPI	GGTGA	3 cut(s)	10, 344, 865
AvaII	GGWCC	1 cut(s)	937
BaeGI	GKGCMC	1 cut(s)	125
BanII	GRGCYC	1 cut(s)	18
BauI	CACGAG	1 cut(s)	399
BbsI	GAAGAC	1 cut(s)	202
Bbv12I	GWGCWC	2 cut(s)	18, 76
BccI	CCATC	2 cut(s)	833, 911
BceAI	ACGGC	1 cut(s)	868
BciT130I	CCWGG	1 cut(s)	678
BcoDI	GTCTC	1 cut(s)	778
BfaI	CTAG	2 cut(s)	440, 842
BfmI	CTRYAG	1 cut(s)	391
BfrI	CTTAAG	1 cut(s)	290
Bme1390I	CCNGG	1 cut(s)	678
Bme18I	GGWCC	1 cut(s)	937
BmgT120I	GGNCC	1 cut(s)	937
BmiI	GGNNCC	1 cut(s)	437
BmrFI	CCNGG	1 cut(s)	678
BmsI	GCATC	2 cut(s)	591, 898
BpiI	GAAGAC	1 cut(s)	202
BplI	GAGNNNNNCTC	3 cut(s)	32, 79, 111
Bsa29I	ATCGAT	1 cut(s)	190
BsaJI	CCNNGG	3 cut(s)	590, 605, 677
Bse118I	RCCGGY	1 cut(s)	814
Bse3DI	GCAATG	1 cut(s)	639
BseBI	CCWGG	1 cut(s)	678
BseCI	ATCGAT	1 cut(s)	190
BseDI	CCNNGG	3 cut(s)	590, 605, 677
BseGI	GGATG	2 cut(s)	190, 922
BseMI	GCAATG	1 cut(s)	639
BseMII	CTCAG	2 cut(s)	123, 261
BseRI	GAGGAG	1 cut(s)	587
BseSI	GKGCMC	1 cut(s)	125
BshVI	ATCGAT	1 cut(s)	190
BsiHKAI	GWGCWC	2 cut(s)	18, 76
BsiSI	CCGG	1 cut(s)	815
BsmAI	GTCTC	1 cut(s)	778
Bsp1286I	GDGCHC	3 cut(s)	18, 76, 125
Bsp143I	GATC	2 cut(s)	187, 697
BspCNI	CTCAG	2 cut(s)	124, 262
BspDI	ATCGAT	1 cut(s)	190
BspLI	GGNNCC	1 cut(s)	437
BspTI	CTTAAG	1 cut(s)	290
BsrDI	GCAATG	1 cut(s)	639
BsrFI	RCCGGY	1 cut(s)	814
BssAI	RCCGGY	1 cut(s)	814
BssECI	CCNNGG	3 cut(s)	590, 605, 677
BssMI	GATC	2 cut(s)	187, 697
BssSI	CACGAG	1 cut(s)	399
BssT1I	CCWWGG	1 cut(s)	605
Bst2BI	CACGAG	1 cut(s)	399
Bst2UI	CCWGG	1 cut(s)	678
Bst4CI	ACNGT	7 cut(s)	47, 127, 265, 395, 466, 541, 591
BstAFI	CTTAAG	1 cut(s)	290
BstDEI	CTNAG	3 cut(s)	132, 270, 408
BstDSI	CCRYGG	1 cut(s)	590
BstF5I	GGATG	2 cut(s)	190, 922
BstKTI	GATC	2 cut(s)	190, 700
BstMAI	GTCTC	1 cut(s)	778
BstMBI	GATC	2 cut(s)	187, 697
BstMWI	GCNNNNNNNGC	2 cut(s)	22, 486
BstNI	CCWGG	1 cut(s)	678
BstSCI	CCNGG	1 cut(s)	676
BstSFI	CTRYAG	1 cut(s)	391
BstSLI	GKGCMC	1 cut(s)	125
BstV2I	GAAGAC	1 cut(s)	202
Bsu15I	ATCGAT	1 cut(s)	190
BsuTUI	ATCGAT	1 cut(s)	190
BtgI	CCRYGG	1 cut(s)	590
BtsCI	GGATG	2 cut(s)	190, 922
BtsIMutI	CAGTG	2 cut(s)	123, 546
Cfr10I	RCCGGY	1 cut(s)	814
Cfr13I	GGNCC	1 cut(s)	937
ClaI	ATCGAT	1 cut(s)	190
Csp6I	GTAC	2 cut(s)	520, 587
CviAII	CATG	3 cut(s)	98, 314, 483
CviQI	GTAC	2 cut(s)	520, 587
DdeI	CTNAG	3 cut(s)	132, 270, 408
DpnI	GATC	2 cut(s)	189, 699
DpnII	GATC	2 cut(s)	187, 697
DraIII	CACNNNGTG	1 cut(s)	350
Ecl136II	GAGCTC	1 cut(s)	16
Eco130I	CCWWGG	1 cut(s)	605
Eco24I	GRGCYC	1 cut(s)	18
Eco32I	GATATC	1 cut(s)	772
Eco47I	GGWCC	1 cut(s)	937
Eco53kI	GAGCTC	1 cut(s)	16
Eco57I	CTGAAG	2 cut(s)	491, 684
EcoICRI	GAGCTC	1 cut(s)	16
EcoO109I	RGGNCCY	1 cut(s)	937
EcoRI	GAATTC	1 cut(s)	806
EcoRII	CCWGG	1 cut(s)	676
EcoRV	GATATC	1 cut(s)	772
EcoT14I	CCWWGG	1 cut(s)	605
EcoT38I	GRGCYC	1 cut(s)	18
ErhI	CCWWGG	1 cut(s)	605
FaeI	CATG	3 cut(s)	101, 317, 486
FalI	AAGNNNNNCTT	4 cut(s)	611, 643, 881, 913
FatI	CATG	3 cut(s)	97, 313, 482
FblI	GTMKAC	1 cut(s)	501
FokI	GGATG	2 cut(s)	197, 929
FriOI	GRGCYC	1 cut(s)	18
FspBI	CTAG	2 cut(s)	440, 842
HapII	CCGG	1 cut(s)	815
Hin1II	CATG	3 cut(s)	101, 317, 486
HincII	GTYRAC	2 cut(s)	342, 502
HindII	GTYRAC	2 cut(s)	342, 502
HindIII	AAGCTT	2 cut(s)	527, 950
HinfI	GANTC	6 cut(s)	175, 229, 284, 757, 786, 838
HpaII	CCGG	1 cut(s)	815
HphI	GGTGA	3 cut(s)	10, 344, 865
Hpy166II	GTNNAC	5 cut(s)	342, 502, 522, 551, 674
Hpy188I	TCNGA	2 cut(s)	510, 697
Hpy188III	TCNNGA	5 cut(s)	111, 226, 236, 364, 767
Hpy8I	GTNNAC	5 cut(s)	342, 502, 522, 551, 674
HpyAV	CCTTC	3 cut(s)	515, 659, 731
HpyCH4III	ACNGT	7 cut(s)	47, 127, 265, 395, 466, 541, 591
HpyCH4IV	ACGT	1 cut(s)	670
HpyCH4V	TGCA	2 cut(s)	632, 911
HpyF10VI	GCNNNNNNNGC	2 cut(s)	22, 486
HpyF3I	CTNAG	3 cut(s)	132, 270, 408
HpySE526I	ACGT	1 cut(s)	670
Hsp92II	CATG	3 cut(s)	101, 317, 486
Kzo9I	GATC	2 cut(s)	187, 697
LmnI	GCTCC	1 cut(s)	65
LweI	GCATC	2 cut(s)	591, 898
MaeI	CTAG	2 cut(s)	440, 842
MaeII	ACGT	1 cut(s)	670
MaeIII	GTNAC	2 cut(s)	172, 196
MalI	GATC	2 cut(s)	189, 699
MboI	GATC	2 cut(s)	187, 697
MboII	GAAGA	5 cut(s)	93, 202, 224, 889, 901
MhlI	GDGCHC	3 cut(s)	18, 76, 125
MluCI	AATT	5 cut(s)	51, 114, 511, 742, 806
MlyI	GAGTC	2 cut(s)	169, 780
MmeI	TCCRAC	1 cut(s)	162
MnlI	CCTC	9 cut(s)	470, 565, 568, 649, 785, 827, 844, 907, 928
MroXI	GAANNNNTTC	2 cut(s)	893, 896
MseI	TTAA	5 cut(s)	117, 291, 300, 420, 955
MspCI	CTTAAG	1 cut(s)	290
MspI	CCGG	1 cut(s)	815
MspR9I	CCNGG	1 cut(s)	678
MvaI	CCWGG	1 cut(s)	678
MwoI	GCNNNNNNNGC	2 cut(s)	22, 486
NdeII	GATC	2 cut(s)	187, 697
NlaIII	CATG	3 cut(s)	101, 317, 486
NlaIV	GGNNCC	1 cut(s)	437
NmuCI	GTSAC	1 cut(s)	172
PdmI	GAANNNNTTC	2 cut(s)	893, 896
PfeI	GAWTC	4 cut(s)	229, 284, 757, 838
PleI	GAGTC	2 cut(s)	169, 780
PpsI	GAGTC	2 cut(s)	169, 780
PpuMI	RGGWCCY	1 cut(s)	937
PsiI	TTATAA	1 cut(s)	516
Psp124BI	GAGCTC	1 cut(s)	18
Psp5II	RGGWCCY	1 cut(s)	937
Psp6I	CCWGG	1 cut(s)	676
PspGI	CCWGG	1 cut(s)	676
PspN4I	GGNNCC	1 cut(s)	437
PspPI	GGNCC	1 cut(s)	937
PspPPI	RGGWCCY	1 cut(s)	937
PsrI	GAACNNNNNNTAC	2 cut(s)	884, 916
RsaI	GTAC	2 cut(s)	521, 588
RsaNI	GTAC	2 cut(s)	520, 587
SacI	GAGCTC	1 cut(s)	18
SalI	GTCGAC	1 cut(s)	500
SaqAI	TTAA	5 cut(s)	117, 291, 300, 420, 955
Sau3AI	GATC	2 cut(s)	187, 697
Sau96I	GGNCC	1 cut(s)	937
SchI	GAGTC	2 cut(s)	169, 780
ScrFI	CCNGG	1 cut(s)	678
SduI	GDGCHC	3 cut(s)	18, 76, 125
SfaNI	GCATC	2 cut(s)	591, 898
SfcI	CTRYAG	1 cut(s)	391
SinI	GGWCC	1 cut(s)	937
SmlI	CTYRAG	1 cut(s)	290
SmoI	CTYRAG	1 cut(s)	290
Sse9I	AATT	5 cut(s)	51, 114, 511, 742, 806
SspI	AATATT	1 cut(s)	495
SspMI	CTAG	2 cut(s)	440, 842
SstI	GAGCTC	1 cut(s)	18
StyD4I	CCNGG	1 cut(s)	676
StyI	CCWWGG	1 cut(s)	605
TaaI	ACNGT	7 cut(s)	47, 127, 265, 395, 466, 541, 591
TaiI	ACGT	1 cut(s)	673
TaqI	TCGA	4 cut(s)	190, 363, 501, 933
TaqII	GACCGA	1 cut(s)	736
TasI	AATT	5 cut(s)	51, 114, 511, 742, 806
TatI	WGTACW	1 cut(s)	519
TfiI	GAWTC	4 cut(s)	229, 284, 757, 838
Tru1I	TTAA	5 cut(s)	117, 291, 300, 420, 955
Tru9I	TTAA	5 cut(s)	117, 291, 300, 420, 955
TscAI	CASTG	2 cut(s)	130, 546
TseFI	GTSAC	1 cut(s)	172
Tsp45I	GTSAC	1 cut(s)	172
TspDTI	ATGAA	4 cut(s)	255, 302, 438, 549
TspGWI	ACGGA	2 cut(s)	21, 218
TspRI	CASTG	2 cut(s)	130, 546
Vha464I	CTTAAG	1 cut(s)	290
VpaK11BI	GGWCC	1 cut(s)	937
XapI	RAATTY	2 cut(s)	742, 806
XmiI	GTMKAC	1 cut(s)	501
XmnI	GAANNNNTTC	2 cut(s)	893, 896
XspI	CTAG	2 cut(s)	440, 842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.