Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008631

Physical Location & Seq

Genomic Coordinates Forward (+)

20618733 .. 20621103

Gene Structure 2371 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G50680.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGACGGAGATGAGCTCACCGAGCAGGAAACTGCTCTTTATGACCGTCAAATTAGGGTATGGGGAGCTGGTGCTCAAAGAAGATTGAGTAAATCTCATGTTTTGGTATCTGGAATTAAGGGCACTGTTGCTGAGTTTTGTAAGAACATTGTGTTGGCAGGAGTAGGTAGTGTGACTCTGTTGGATGATCGATTGGTTACAACGGAAGTCTTCAACGCAAACTTCTTGATTCTTCCTGATGAAAATGCTTATGTAGGCAAAACCGTAGCTGAGATTTGTTGTGATTCACTTAAGGATTTTAACCCTATGGTTCATGTTTCAATAGAGAAAGGTGATTTGTCAACACTTGGTGTTGATTTTTTCGAGAAGTTTGATGTTGTGGTTATCGGCTACAGTTCTCGTGCTACTAAGAAAGCAGTTAATGAAAAGTGTAGGAACCTAGCAAAAGATGTAGCGTTCTATACAGTTGATTGTAGAGGCTCATGTGGCGAAATATTTGTCGACCTTCAGAATTATAAGTACACAAAGAAAAAGCTTGATGAAACAGTGGAATGTGAACTAACATTTCCAAGTTTTGAGGAGGCAGTTTCAGTACCGTGGAAACCGATGCCAAGGAGAACAGCAAAGCTCTACTTTGCAATGAGAGTGATAGAACTATTTGAGGAAACTGAAGGACGTAAACCTGGGGAATGTTCGCTGTCTGATCTCCCAAGAGTTTTGAAACTCAAAAAGGAACTATGTGAAGGAAATTCGGTCAGCGAGAATCATATTCCAGATATCCTCTTGGAGAGACTCGTATCTAATAACACAGAATTCCCACCGGCTTGTGCCATCATTGGAGGGATTCTAGGACAGGAGGTGATAAAAGTTATATCAGGCAAAGGAGAGCCGTTGAAGAACTTCTTCTACTTTGATGCAGAGGATGGGAAAGGTGTAATCGAGGACCTATCCCACAAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008641 GO:0009987 GO:0016874 GO:0016877 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0019948 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

ThiF

Protein Analysis

320

Amino Acids

35.65

Weight (kDa)

5.15

Isoelectric Point (pI)

37.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	14 - 304	1e-24	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008631)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24940 AT5G50580 AT5G50580 AT5G50680 AT5G50680
fragaria_vesca	FvH4_5g08600 FvH4_5g08600 FvH4_5g08600 FvH4_5g08600
malus_domestica	MD06G1138700.v1.1 MD14G1154700.v1.1
prunus_persica	Prupe.5G148900_v2.0.a1 Prupe.5G148900_v2.0.a1
pyrus_communis	pycom06g12980 pycom14g12870
rosa_chinensis	RchiOBHm_Chr7g0189801
rosa_laevigata	RLG00000004589
rosa_multiflora	Rmu_sc0014337.1_g000003
rosa_roxburghii	Rroxscaffold_3G00265150
rosa_rugosa	Rorug06G0500000
rosa_samantha	Rh7AG105100 Rh7CG109700
rosa_wichuraiana	Rw7G009100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	516
AccI	GTMKAC	1 cut(s)	501
AcsI	RAATTY	2 cut(s)	748, 812
AcuI	CTGAAG	2 cut(s)	491, 690
AdeI	CACNNNGTG	1 cut(s)	350
AfaI	GTAC	2 cut(s)	521, 594
AflII	CTTAAG	1 cut(s)	290
AgsI	TTSAA	4 cut(s)	214, 321, 721, 895
AjnI	CCWGG	1 cut(s)	682
AjuI	GAANNNNNNNTTGG	2 cut(s)	137, 169
AluBI	AGCT	6 cut(s)	16, 68, 269, 535, 628, 958
AluI	AGCT	6 cut(s)	16, 68, 269, 535, 628, 958
Alw21I	GWGCWC	2 cut(s)	18, 76
Alw26I	GTCTC	1 cut(s)	784
ApoI	RAATTY	2 cut(s)	748, 812
ArsI	GACNNNNNNTTYG	2 cut(s)	483, 515
Asp700I	GAANNNNTTC	2 cut(s)	899, 902
AspS9I	GGNCC	1 cut(s)	943
AsuHPI	GGTGA	3 cut(s)	10, 344, 871
AvaII	GGWCC	1 cut(s)	943
BaeGI	GKGCMC	1 cut(s)	125
BanII	GRGCYC	1 cut(s)	18
BauI	CACGAG	1 cut(s)	399
BbsI	GAAGAC	1 cut(s)	202
Bbv12I	GWGCWC	2 cut(s)	18, 76
BccI	CCATC	2 cut(s)	839, 917
BceAI	ACGGC	1 cut(s)	874
BciT130I	CCWGG	1 cut(s)	684
BcoDI	GTCTC	1 cut(s)	784
BfaI	CTAG	2 cut(s)	440, 848
BfmI	CTRYAG	1 cut(s)	391
BfrI	CTTAAG	1 cut(s)	290
Bme1390I	CCNGG	1 cut(s)	684
Bme18I	GGWCC	1 cut(s)	943
BmgT120I	GGNCC	1 cut(s)	943
BmiI	GGNNCC	1 cut(s)	437
BmrFI	CCNGG	1 cut(s)	684
BmsI	GCATC	2 cut(s)	597, 904
BpiI	GAAGAC	1 cut(s)	202
BplI	GAGNNNNNCTC	3 cut(s)	32, 79, 111
Bsa29I	ATCGAT	1 cut(s)	190
BsaJI	CCNNGG	3 cut(s)	596, 611, 683
Bse118I	RCCGGY	1 cut(s)	820
Bse3DI	GCAATG	1 cut(s)	645
BseBI	CCWGG	1 cut(s)	684
BseCI	ATCGAT	1 cut(s)	190
BseDI	CCNNGG	3 cut(s)	596, 611, 683
BseGI	GGATG	2 cut(s)	190, 928
BseMI	GCAATG	1 cut(s)	645
BseMII	CTCAG	2 cut(s)	123, 261
BseRI	GAGGAG	1 cut(s)	593
BseSI	GKGCMC	1 cut(s)	125
BshVI	ATCGAT	1 cut(s)	190
BsiHKAI	GWGCWC	2 cut(s)	18, 76
BsiSI	CCGG	1 cut(s)	821
BsmAI	GTCTC	1 cut(s)	784
Bsp1286I	GDGCHC	3 cut(s)	18, 76, 125
Bsp143I	GATC	2 cut(s)	187, 703
BspCNI	CTCAG	2 cut(s)	124, 262
BspDI	ATCGAT	1 cut(s)	190
BspLI	GGNNCC	1 cut(s)	437
BspTI	CTTAAG	1 cut(s)	290
BsrDI	GCAATG	1 cut(s)	645
BsrFI	RCCGGY	1 cut(s)	820
BssAI	RCCGGY	1 cut(s)	820
BssECI	CCNNGG	3 cut(s)	596, 611, 683
BssMI	GATC	2 cut(s)	187, 703
BssSI	CACGAG	1 cut(s)	399
BssT1I	CCWWGG	1 cut(s)	611
Bst2BI	CACGAG	1 cut(s)	399
Bst2UI	CCWGG	1 cut(s)	684
Bst4CI	ACNGT	7 cut(s)	47, 127, 265, 395, 466, 547, 597
BstAFI	CTTAAG	1 cut(s)	290
BstDEI	CTNAG	3 cut(s)	132, 270, 408
BstDSI	CCRYGG	1 cut(s)	596
BstF5I	GGATG	2 cut(s)	190, 928
BstKTI	GATC	2 cut(s)	190, 706
BstMAI	GTCTC	1 cut(s)	784
BstMBI	GATC	2 cut(s)	187, 703
BstMWI	GCNNNNNNNGC	2 cut(s)	22, 486
BstNI	CCWGG	1 cut(s)	684
BstSCI	CCNGG	1 cut(s)	682
BstSFI	CTRYAG	1 cut(s)	391
BstSLI	GKGCMC	1 cut(s)	125
BstV2I	GAAGAC	1 cut(s)	202
Bsu15I	ATCGAT	1 cut(s)	190
BsuTUI	ATCGAT	1 cut(s)	190
BtgI	CCRYGG	1 cut(s)	596
BtsCI	GGATG	2 cut(s)	190, 928
BtsIMutI	CAGTG	2 cut(s)	123, 552
Cfr10I	RCCGGY	1 cut(s)	820
Cfr13I	GGNCC	1 cut(s)	943
ClaI	ATCGAT	1 cut(s)	190
Csp6I	GTAC	2 cut(s)	520, 593
CviAII	CATG	3 cut(s)	98, 314, 483
CviQI	GTAC	2 cut(s)	520, 593
DdeI	CTNAG	3 cut(s)	132, 270, 408
DpnI	GATC	2 cut(s)	189, 705
DpnII	GATC	2 cut(s)	187, 703
DraIII	CACNNNGTG	1 cut(s)	350
Ecl136II	GAGCTC	1 cut(s)	16
Eco130I	CCWWGG	1 cut(s)	611
Eco24I	GRGCYC	1 cut(s)	18
Eco32I	GATATC	1 cut(s)	778
Eco47I	GGWCC	1 cut(s)	943
Eco53kI	GAGCTC	1 cut(s)	16
Eco57I	CTGAAG	2 cut(s)	491, 690
EcoICRI	GAGCTC	1 cut(s)	16
EcoO109I	RGGNCCY	1 cut(s)	943
EcoRI	GAATTC	1 cut(s)	812
EcoRII	CCWGG	1 cut(s)	682
EcoRV	GATATC	1 cut(s)	778
EcoT14I	CCWWGG	1 cut(s)	611
EcoT38I	GRGCYC	1 cut(s)	18
ErhI	CCWWGG	1 cut(s)	611
FaeI	CATG	3 cut(s)	101, 317, 486
FalI	AAGNNNNNCTT	4 cut(s)	617, 649, 887, 919
FatI	CATG	3 cut(s)	97, 313, 482
FblI	GTMKAC	1 cut(s)	501
FokI	GGATG	2 cut(s)	197, 935
FriOI	GRGCYC	1 cut(s)	18
FspBI	CTAG	2 cut(s)	440, 848
HapII	CCGG	1 cut(s)	821
Hin1II	CATG	3 cut(s)	101, 317, 486
HincII	GTYRAC	2 cut(s)	342, 502
HindII	GTYRAC	2 cut(s)	342, 502
HindIII	AAGCTT	2 cut(s)	533, 956
HinfI	GANTC	6 cut(s)	175, 229, 284, 763, 792, 844
HpaII	CCGG	1 cut(s)	821
HphI	GGTGA	3 cut(s)	10, 344, 871
Hpy166II	GTNNAC	5 cut(s)	342, 502, 522, 557, 680
Hpy188I	TCNGA	2 cut(s)	510, 703
Hpy188III	TCNNGA	5 cut(s)	111, 226, 236, 364, 773
Hpy8I	GTNNAC	5 cut(s)	342, 502, 522, 557, 680
HpyAV	CCTTC	3 cut(s)	515, 665, 737
HpyCH4III	ACNGT	7 cut(s)	47, 127, 265, 395, 466, 547, 597
HpyCH4IV	ACGT	1 cut(s)	676
HpyCH4V	TGCA	2 cut(s)	638, 917
HpyF10VI	GCNNNNNNNGC	2 cut(s)	22, 486
HpyF3I	CTNAG	3 cut(s)	132, 270, 408
HpySE526I	ACGT	1 cut(s)	676
Hsp92II	CATG	3 cut(s)	101, 317, 486
Kzo9I	GATC	2 cut(s)	187, 703
LmnI	GCTCC	1 cut(s)	65
LweI	GCATC	2 cut(s)	597, 904
MaeI	CTAG	2 cut(s)	440, 848
MaeII	ACGT	1 cut(s)	676
MaeIII	GTNAC	2 cut(s)	172, 196
MalI	GATC	2 cut(s)	189, 705
MboI	GATC	2 cut(s)	187, 703
MboII	GAAGA	5 cut(s)	93, 202, 224, 895, 907
MhlI	GDGCHC	3 cut(s)	18, 76, 125
MluCI	AATT	5 cut(s)	51, 114, 511, 748, 812
MlyI	GAGTC	2 cut(s)	169, 786
MmeI	TCCRAC	1 cut(s)	162
MnlI	CCTC	9 cut(s)	470, 571, 574, 655, 791, 833, 850, 913, 934
MroXI	GAANNNNTTC	2 cut(s)	899, 902
MseI	TTAA	5 cut(s)	117, 291, 300, 420, 961
MspCI	CTTAAG	1 cut(s)	290
MspI	CCGG	1 cut(s)	821
MspR9I	CCNGG	1 cut(s)	684
MvaI	CCWGG	1 cut(s)	684
MwoI	GCNNNNNNNGC	2 cut(s)	22, 486
NdeII	GATC	2 cut(s)	187, 703
NlaIII	CATG	3 cut(s)	101, 317, 486
NlaIV	GGNNCC	1 cut(s)	437
NmuCI	GTSAC	1 cut(s)	172
PdmI	GAANNNNTTC	2 cut(s)	899, 902
PfeI	GAWTC	4 cut(s)	229, 284, 763, 844
PleI	GAGTC	2 cut(s)	169, 786
PpsI	GAGTC	2 cut(s)	169, 786
PpuMI	RGGWCCY	1 cut(s)	943
PsiI	TTATAA	1 cut(s)	516
Psp124BI	GAGCTC	1 cut(s)	18
Psp5II	RGGWCCY	1 cut(s)	943
Psp6I	CCWGG	1 cut(s)	682
PspGI	CCWGG	1 cut(s)	682
PspN4I	GGNNCC	1 cut(s)	437
PspPI	GGNCC	1 cut(s)	943
PspPPI	RGGWCCY	1 cut(s)	943
PsrI	GAACNNNNNNTAC	2 cut(s)	890, 922
RsaI	GTAC	2 cut(s)	521, 594
RsaNI	GTAC	2 cut(s)	520, 593
SacI	GAGCTC	1 cut(s)	18
SalI	GTCGAC	1 cut(s)	500
SaqAI	TTAA	5 cut(s)	117, 291, 300, 420, 961
Sau3AI	GATC	2 cut(s)	187, 703
Sau96I	GGNCC	1 cut(s)	943
SchI	GAGTC	2 cut(s)	169, 786
ScrFI	CCNGG	1 cut(s)	684
SduI	GDGCHC	3 cut(s)	18, 76, 125
SfaNI	GCATC	2 cut(s)	597, 904
SfcI	CTRYAG	1 cut(s)	391
SinI	GGWCC	1 cut(s)	943
SmlI	CTYRAG	1 cut(s)	290
SmoI	CTYRAG	1 cut(s)	290
Sse9I	AATT	5 cut(s)	51, 114, 511, 748, 812
SspI	AATATT	1 cut(s)	495
SspMI	CTAG	2 cut(s)	440, 848
SstI	GAGCTC	1 cut(s)	18
StyD4I	CCNGG	1 cut(s)	682
StyI	CCWWGG	1 cut(s)	611
TaaI	ACNGT	7 cut(s)	47, 127, 265, 395, 466, 547, 597
TaiI	ACGT	1 cut(s)	679
TaqI	TCGA	4 cut(s)	190, 363, 501, 939
TaqII	GACCGA	1 cut(s)	742
TasI	AATT	5 cut(s)	51, 114, 511, 748, 812
TatI	WGTACW	1 cut(s)	519
TfiI	GAWTC	4 cut(s)	229, 284, 763, 844
Tru1I	TTAA	5 cut(s)	117, 291, 300, 420, 961
Tru9I	TTAA	5 cut(s)	117, 291, 300, 420, 961
TscAI	CASTG	2 cut(s)	130, 552
TseFI	GTSAC	1 cut(s)	172
Tsp45I	GTSAC	1 cut(s)	172
TspDTI	ATGAA	4 cut(s)	255, 302, 438, 555
TspGWI	ACGGA	2 cut(s)	21, 218
TspRI	CASTG	2 cut(s)	130, 552
Vha464I	CTTAAG	1 cut(s)	290
VpaK11BI	GGWCC	1 cut(s)	943
XapI	RAATTY	2 cut(s)	748, 812
XmiI	GTMKAC	1 cut(s)	501
XmnI	GAANNNNTTC	2 cut(s)	899, 902
XspI	CTAG	2 cut(s)	440, 848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.