Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0008631

Physical Location & Seq

Genomic Coordinates Reverse (-)

28359789 .. 28363351

Gene Structure 3563 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1138700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGACAAATCTCTCCAATTCCTCGGCCCCAGCAAACGGTTTGGGCTACCAAAATCTTACGGGTAACTTCATACGAACCCAAAAATGGGGCCCAACTAAGACGAATGAAAAGGCCCAATCCAAGTTGGGTCAAAATAAAGCGAAAAAAGTCCGAATATATGCCTCGAAAATCAAAATTCCCGAAGTTCAATCGGAAGCCCCTCGTCACTGTCCTTCTGTGCAATTCAAATTCTCAGTTTTTCTAGGGTTGTGTTTGGGAATGGATGGCGAGGAGTTGACTGAGCAGGAGACTGCACTTTACGATCGCCAAATTAGGGTTTGGGGCGCTGGTGCCCAAAGAAGGCTTAGCAAAGCTCATGTATTGGTCTGTGGAATTACTGGAACTATTGCCGAGTTTTGCAAGAACATTGTCCTAGCCGGAGTTGGTAGTTTGACACTGGTGGATGATCGGGTAGTGACCGAAGACGCACTTTCTGCCAACTTTTTGATACCTTCTGATGAAAATGTGTATGCTGGGAAAACTCTGACTGAGCTTTGCCGTGATTCTCTGAAAGATTTCAATCCAATGGTTCGTGTTTCTGTTGAAAAAGGTGACTTGTCAAGCTTTGGGTCAGAGTTCTATGGTAAATTTGATGTCGTAGTTGTCAGCTGTTGCTCCTTCACAACTAAGAAATTGATCAATGAAAAATGCCGAAAGTCCTCGAAGCGTGTAGCGTTTTATACAGTTGATTGTAGAGATTCCTGTGGTGAAATATTTGTTGATCTGCAACACCATAAATATTCGAAGAAAAAAAATGAGGAAACAATCGAATGCGAACTACATTATCCAAGCTTTGAGGAATCAATTTCAGTACCTTGGAAATCATTTCCCAGGAGATTCTCAAAGCAATACTTTGCTATGCGAGTAATTGAAAGGTTTGAAGAAGCCGAGCAACGTAAACCTGGAGAGTTATCGATTGCAGATCTTCCTGCTGTTCTGAAGCTGAAAAAAGAACTCTGTGAAGCACAGTCACTGAATGAGTCTCATATTCCAAATGCGCTCCTGGAAAGATTGGTGACGGATACAAGAGAGTTTCCTCCGGTTTGTCCCATCATTGGAGGGATTCTTGGGCAGGAGGTAATTAAGACAATATCAGAGAAAGGAGACCCTGTCAAGAATTTCTTCTTCTTCGATGCTATGGATGGAAAGGGCATAATCGAGGACGTATCAGGGAACCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008641 GO:0009987 GO:0016874 GO:0016877 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0019948 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

ThiF

Protein Analysis

407

Amino Acids

45.4

Weight (kDa)

7.87

Isoelectric Point (pI)

40.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	100 - 396	1.3e-24	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008631)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24940 AT5G50580 AT5G50580 AT5G50680 AT5G50680
fragaria_vesca	FvH4_5g08600 FvH4_5g08600 FvH4_5g08600 FvH4_5g08600
malus_domestica	MD06G1138700.v1.1 MD14G1154700.v1.1
prunus_persica	Prupe.5G148900_v2.0.a1 Prupe.5G148900_v2.0.a1
pyrus_communis	pycom06g12980 pycom14g12870
rosa_chinensis	RchiOBHm_Chr7g0189801
rosa_laevigata	RLG00000004589
rosa_multiflora	Rmu_sc0014337.1_g000003
rosa_roxburghii	Rroxscaffold_3G00265150
rosa_rugosa	Rorug06G0500000
rosa_samantha	Rh7AG105100 Rh7CG109700
rosa_wichuraiana	Rw7G009100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	329
AcsI	RAATTY	4 cut(s)	174, 227, 626, 1156
AcuI	CTGAAG	1 cut(s)	998
AfaI	GTAC	1 cut(s)	852
AfiI	CCNNNNNNNGG	6 cut(s)	35, 84, 85, 313, 339, 1094
AgsI	TTSAA	6 cut(s)	188, 226, 559, 584, 911, 920
AjnI	CCWGG	3 cut(s)	869, 940, 1041
AluBI	AGCT	6 cut(s)	353, 532, 603, 648, 831, 982
AluI	AGCT	6 cut(s)	353, 532, 603, 648, 831, 982
Alw26I	GTCTC	3 cut(s)	281, 1026, 1137
AlwNI	CAGNNNCTG	1 cut(s)	1012
AoxI	GGCC	3 cut(s)	24, 88, 111
ApaI	GGGCCC	1 cut(s)	92
ApoI	RAATTY	4 cut(s)	174, 227, 626, 1156
Asp700I	GAANNNNTTC	2 cut(s)	554, 1160
AspLEI	GCGC	2 cut(s)	326, 1039
AspS9I	GGNCC	4 cut(s)	25, 88, 89, 112
AsuHPI	GGTGA	3 cut(s)	602, 758, 1066
AsuII	TTCGAA	1 cut(s)	782
BaeGI	GKGCMC	2 cut(s)	92, 334
BanI	GGYRCC	1 cut(s)	329
BanII	GRGCYC	1 cut(s)	92
BbsI	GAAGAC	1 cut(s)	468
BccI	CCATC	3 cut(s)	257, 1097, 1175
BceAI	ACGGC	1 cut(s)	522
BciT130I	CCWGG	3 cut(s)	871, 942, 1043
BciVI	GTATCC	1 cut(s)	1054
BclI	TGATCA	1 cut(s)	675
BcoDI	GTCTC	3 cut(s)	281, 1026, 1137
BfaI	CTAG	2 cut(s)	242, 413
BfoI	RGCGCY	1 cut(s)	327
BfuI	GTATCC	1 cut(s)	1054
BglII	AGATCT	1 cut(s)	961
BlpI	GCTNAGC	1 cut(s)	344
Bme1390I	CCNGG	3 cut(s)	871, 942, 1043
BmgT120I	GGNCC	4 cut(s)	25, 88, 89, 112
BmiI	GGNNCC	5 cut(s)	27, 89, 90, 331, 1214
BmrFI	CCNGG	3 cut(s)	871, 942, 1043
BmsI	GCATC	1 cut(s)	1162
BpiI	GAAGAC	1 cut(s)	468
BpmI	CTGGAG	1 cut(s)	963
Bpu1102I	GCTNAGC	1 cut(s)	344
Bpu14I	TTCGAA	1 cut(s)	782
Bsa29I	ATCGAT	1 cut(s)	953
BsaBI	GATNNNNATC	1 cut(s)	558
BsaI	GGTCTC	1 cut(s)	1137
BsaJI	CCNNGG	3 cut(s)	21, 854, 869
BsaWI	WCCGGW	1 cut(s)	1078
BsaXI	ACNNNNNCTCC	2 cut(s)	1134, 1164
Bsc4I	CCNNNNNNNGG	6 cut(s)	35, 84, 85, 313, 339, 1094
Bse1I	ACTGG	2 cut(s)	382, 441
Bse8I	GATNNNNATC	1 cut(s)	558
BseBI	CCWGG	3 cut(s)	871, 942, 1043
BseCI	ATCGAT	1 cut(s)	953
BseDI	CCNNGG	3 cut(s)	21, 854, 869
BseGI	GGATG	3 cut(s)	268, 448, 1186
BseJI	GATNNNNATC	1 cut(s)	558
BseLI	CCNNNNNNNGG	6 cut(s)	35, 84, 85, 313, 339, 1094
BseMII	CTCAG	3 cut(s)	246, 270, 519
BseNI	ACTGG	2 cut(s)	382, 441
BseRI	GAGGAG	1 cut(s)	284
BseSI	GKGCMC	2 cut(s)	92, 334
BseYI	CCCAGC	2 cut(s)	28, 512
BsgI	GTGCAG	1 cut(s)	276
Bsh1285I	CGRYCG	1 cut(s)	304
BshFI	GGCC	3 cut(s)	26, 90, 113
BshNI	GGYRCC	1 cut(s)	329
BshVI	ATCGAT	1 cut(s)	953
BsiEI	CGRYCG	1 cut(s)	304
BsiSI	CCGG	2 cut(s)	417, 1079
BslFI	GGGAC	1 cut(s)	1071
BslI	CCNNNNNNNGG	6 cut(s)	35, 84, 85, 313, 339, 1094
BsmAI	GTCTC	3 cut(s)	281, 1026, 1137
BsmFI	GGGAC	1 cut(s)	1071
BsmI	GAATGC	1 cut(s)	815
BsnI	GGCC	3 cut(s)	26, 90, 113
Bso31I	GGTCTC	1 cut(s)	1137
Bsp119I	TTCGAA	1 cut(s)	782
Bsp120I	GGGCCC	1 cut(s)	88
Bsp1286I	GDGCHC	2 cut(s)	92, 334
Bsp143I	GATC	5 cut(s)	301, 445, 675, 760, 961
Bsp1720I	GCTNAGC	1 cut(s)	344
BspANI	GGCC	3 cut(s)	26, 90, 113
BspCNI	CTCAG	3 cut(s)	245, 271, 520
BspDI	ATCGAT	1 cut(s)	953
BspLI	GGNNCC	5 cut(s)	27, 89, 90, 331, 1214
BspT104I	TTCGAA	1 cut(s)	782
BspT107I	GGYRCC	1 cut(s)	329
BspTNI	GGTCTC	1 cut(s)	1137
BsrI	ACTGG	2 cut(s)	382, 441
BssECI	CCNNGG	3 cut(s)	21, 854, 869
BssMI	GATC	5 cut(s)	301, 445, 675, 760, 961
BssT1I	CCWWGG	1 cut(s)	854
Bst2UI	CCWGG	3 cut(s)	871, 942, 1043
Bst4CI	ACNGT	4 cut(s)	38, 209, 724, 1008
BstAPI	GCANNNNNTGC	1 cut(s)	473
BstBI	TTCGAA	1 cut(s)	782
BstDEI	CTNAG	6 cut(s)	96, 232, 279, 344, 528, 666
BstF5I	GGATG	3 cut(s)	268, 448, 1186
BstH2I	RGCGCY	1 cut(s)	327
BstHHI	GCGC	2 cut(s)	326, 1039
BstKTI	GATC	5 cut(s)	304, 448, 678, 763, 964
BstMAI	GTCTC	3 cut(s)	281, 1026, 1137
BstMBI	GATC	5 cut(s)	301, 445, 675, 760, 961
BstMCI	CGRYCG	1 cut(s)	304
BstMWI	GCNNNNNNNGC	1 cut(s)	473
BstNI	CCWGG	3 cut(s)	871, 942, 1043
BstSCI	CCNGG	3 cut(s)	869, 940, 1041
BstSLI	GKGCMC	2 cut(s)	92, 334
BstV2I	GAAGAC	1 cut(s)	468
BstX2I	RGATCY	1 cut(s)	961
BstYI	RGATCY	1 cut(s)	961
Bsu15I	ATCGAT	1 cut(s)	953
BsuI	GTATCC	1 cut(s)	1054
BsuRI	GGCC	3 cut(s)	26, 90, 113
BsuTUI	ATCGAT	1 cut(s)	953
BtsCI	GGATG	3 cut(s)	268, 448, 1186
BtsIMutI	CAGTG	3 cut(s)	205, 434, 1010
CaiI	CAGNNNCTG	1 cut(s)	1012
CfoI	GCGC	2 cut(s)	326, 1039
Cfr13I	GGNCC	4 cut(s)	25, 88, 89, 112
ClaI	ATCGAT	1 cut(s)	953
CseI	GACGC	1 cut(s)	473
Csp6I	GTAC	1 cut(s)	851
CviAII	CATG	1 cut(s)	356
CviQI	GTAC	1 cut(s)	851
DdeI	CTNAG	6 cut(s)	96, 232, 279, 344, 528, 666
DpnI	GATC	5 cut(s)	303, 447, 677, 762, 963
DpnII	GATC	5 cut(s)	301, 445, 675, 760, 961
Eco130I	CCWWGG	1 cut(s)	854
Eco24I	GRGCYC	1 cut(s)	92
Eco31I	GGTCTC	1 cut(s)	1137
Eco57I	CTGAAG	1 cut(s)	998
EcoO109I	RGGNCCY	1 cut(s)	88
EcoRII	CCWGG	3 cut(s)	869, 940, 1041
EcoT14I	CCWWGG	1 cut(s)	854
EcoT38I	GRGCYC	1 cut(s)	92
ErhI	CCWWGG	1 cut(s)	854
FaeI	CATG	1 cut(s)	359
FalI	AAGNNNNNCTT	6 cut(s)	453, 485, 875, 907, 1145, 1177
FaqI	GGGAC	1 cut(s)	1071
FatI	CATG	1 cut(s)	355
FbaI	TGATCA	1 cut(s)	675
FokI	GGATG	3 cut(s)	275, 455, 1193
FriOI	GRGCYC	1 cut(s)	92
FspBI	CTAG	2 cut(s)	242, 413
GlaI	GCGC	2 cut(s)	325, 1038
GsaI	CCCAGC	2 cut(s)	32, 516
GsuI	CTGGAG	1 cut(s)	963
HaeII	RGCGCY	1 cut(s)	327
HaeIII	GGCC	3 cut(s)	26, 90, 113
HapII	CCGG	2 cut(s)	417, 1079
HgaI	GACGC	1 cut(s)	473
HhaI	GCGC	2 cut(s)	326, 1039
Hin1II	CATG	1 cut(s)	359
Hin6I	GCGC	2 cut(s)	324, 1037
HinP1I	GCGC	2 cut(s)	324, 1037
HincII	GTYRAC	1 cut(s)	276
HindII	GTYRAC	1 cut(s)	276
HindIII	AAGCTT	2 cut(s)	601, 829
HinfI	GANTC	6 cut(s)	542, 737, 839, 876, 1019, 1102
HpaII	CCGG	2 cut(s)	417, 1079
HphI	GGTGA	3 cut(s)	602, 758, 1066
Hpy166II	GTNNAC	2 cut(s)	276, 938
Hpy188I	TCNGA	8 cut(s)	152, 193, 496, 525, 549, 613, 978, 1135
Hpy188III	TCNNGA	2 cut(s)	179, 1153
Hpy8I	GTNNAC	2 cut(s)	276, 938
HpyAV	CCTTC	4 cut(s)	222, 333, 501, 667
HpyCH4III	ACNGT	4 cut(s)	38, 209, 724, 1008
HpyCH4IV	ACGT	2 cut(s)	934, 1203
HpyCH4V	TGCA	5 cut(s)	220, 293, 399, 766, 959
HpyF10VI	GCNNNNNNNGC	1 cut(s)	473
HpyF3I	CTNAG	6 cut(s)	96, 232, 279, 344, 528, 666
HpySE526I	ACGT	2 cut(s)	934, 1203
Hsp92II	CATG	1 cut(s)	359
HspAI	GCGC	2 cut(s)	324, 1037
Ksp22I	TGATCA	1 cut(s)	675
Kzo9I	GATC	5 cut(s)	301, 445, 675, 760, 961
LmnI	GCTCC	2 cut(s)	659, 1044
LweI	GCATC	1 cut(s)	1162
MaeI	CTAG	2 cut(s)	242, 413
MaeII	ACGT	2 cut(s)	934, 1203
MaeIII	GTNAC	6 cut(s)	62, 203, 454, 590, 1008, 1054
MalI	GATC	5 cut(s)	303, 447, 677, 762, 963
MboI	GATC	5 cut(s)	301, 445, 675, 760, 961
MboII	GAAGA	7 cut(s)	473, 796, 932, 956, 1153, 1156, 1159
MflI	RGATCY	1 cut(s)	961
MhlI	GDGCHC	2 cut(s)	92, 334
MlyI	GAGTC	1 cut(s)	1028
MroXI	GAANNNNTTC	2 cut(s)	554, 1160
MseI	TTAA	1 cut(s)	1122
MspA1I	CMGCKG	1 cut(s)	648
MspI	CCGG	2 cut(s)	417, 1079
MspR9I	CCNGG	3 cut(s)	871, 942, 1043
Mva1269I	GAATGC	1 cut(s)	815
MvaI	CCWGG	3 cut(s)	871, 942, 1043
MwoI	GCNNNNNNNGC	1 cut(s)	473
NdeII	GATC	5 cut(s)	301, 445, 675, 760, 961
NlaIII	CATG	1 cut(s)	359
NlaIV	GGNNCC	5 cut(s)	27, 89, 90, 331, 1214
NmeAIII	GCCGAG	2 cut(s)	415, 952
NmuCI	GTSAC	5 cut(s)	203, 454, 590, 1008, 1054
NspV	TTCGAA	1 cut(s)	782
PctI	GAATGC	1 cut(s)	815
PdmI	GAANNNNTTC	2 cut(s)	554, 1160
PfeI	GAWTC	5 cut(s)	542, 737, 839, 876, 1102
PflFI	GACNNNGTC	1 cut(s)	1148
PfoI	TCCNGGA	1 cut(s)	1041
Ple19I	CGATCG	1 cut(s)	304
PleI	GAGTC	1 cut(s)	1027
PpsI	GAGTC	1 cut(s)	1027
Psp6I	CCWGG	3 cut(s)	869, 940, 1041
PspFI	CCCAGC	2 cut(s)	28, 512
PspGI	CCWGG	3 cut(s)	869, 940, 1041
PspN4I	GGNNCC	5 cut(s)	27, 89, 90, 331, 1214
PspOMI	GGGCCC	1 cut(s)	88
PspPI	GGNCC	4 cut(s)	25, 88, 89, 112
PstNI	CAGNNNCTG	1 cut(s)	1012
PsuI	RGATCY	1 cut(s)	961
PsyI	GACNNNGTC	1 cut(s)	1148
PvuI	CGATCG	1 cut(s)	304
PvuII	CAGCTG	1 cut(s)	648
RsaI	GTAC	1 cut(s)	852
RsaNI	GTAC	1 cut(s)	851
SaqAI	TTAA	1 cut(s)	1122
Sau3AI	GATC	5 cut(s)	301, 445, 675, 760, 961
Sau96I	GGNCC	4 cut(s)	25, 88, 89, 112
SchI	GAGTC	1 cut(s)	1028
ScrFI	CCNGG	3 cut(s)	871, 942, 1043
SduI	GDGCHC	2 cut(s)	92, 334
SfaNI	GCATC	1 cut(s)	1162
SfuI	TTCGAA	1 cut(s)	782
SspI	AATATT	2 cut(s)	753, 779
SspMI	CTAG	2 cut(s)	242, 413
StyD4I	CCNGG	3 cut(s)	869, 940, 1041
StyI	CCWWGG	1 cut(s)	854
TaaI	ACNGT	4 cut(s)	38, 209, 724, 1008
TaiI	ACGT	2 cut(s)	937, 1206
TaqI	TCGA	7 cut(s)	164, 701, 782, 807, 953, 1170, 1197
TaqII	GACCGA	1 cut(s)	473
TfiI	GAWTC	5 cut(s)	542, 737, 839, 876, 1102
Tru1I	TTAA	1 cut(s)	1122
Tru9I	TTAA	1 cut(s)	1122
TscAI	CASTG	3 cut(s)	212, 441, 1017
TseFI	GTSAC	5 cut(s)	203, 454, 590, 1008, 1054
Tsp45I	GTSAC	5 cut(s)	203, 454, 590, 1008, 1054
TspDTI	ATGAA	4 cut(s)	58, 120, 513, 696
TspGWI	ACGGA	1 cut(s)	1073
TspRI	CASTG	3 cut(s)	212, 441, 1017
Tth111I	GACNNNGTC	1 cut(s)	1148
XapI	RAATTY	4 cut(s)	174, 227, 626, 1156
XmnI	GAANNNNTTC	2 cut(s)	554, 1160
XspI	CTAG	2 cut(s)	242, 413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.