MD14G1154700.v1.1

SUMO-activating enzyme subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008631
Physical Location & Seq
Reverse (-)
24917607 .. 24920824
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1154700.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGACGGCGAGGAGTTGACTGAGCAGGAGACTGCTCTGTACGATCGCCAGATTAGGGTTTGGGGTGCCGGTGCCCAAAGAAGACTTAGCAAAGCTCATGTATTAGTCTGTGGAATAACTGGAACTGTTGCAGAGTTTTGTAAGAACATTGTACTAGCCGGAGTTGGTAGTTTGACACTGGTGGATGATCGGGTAGTGACAGAAGACGCGCTGTCTGCCAACTTTCTGATACCTTCTGATGAAAGTGTGTATGCTGGGAAAACTTTGACTGAACTTTGCCGTGATTCTCTGAAAGATTTTAATCCGATGGTTCGCGTTTCTGTAGAAAAAGGTGACTTAACAAGCTTTGGGGTAGAGTTCTATAGCAAATTTGACGTCATCGTTGTCAGCTGTTGCTCCTTCACAACTAAAAAATTGATCAATGAAAAATGCCGAAAGTCTTCAAAGCGTGTAGCATTTTATACTGTTGATTGTAGAGACTCCTGTGGTGAAATATTTGTTGATCTGCAACACCATAAATATTCGAAGAAAAAAAATGAGGAAACAATCGAATGTGAACTACAGTATCCAAGCTTTGAGGAATCAATTTCAGTACCTTGGAAATCATTTCCCAGGAGATTCTCAAAGCTATACTTTGCTATGCGAGTAATAGAAAGGTTTGAAGAAGCCGAGCAACGTAAACCTGGAGAGTTATCAATTGCAGATCTTCCTTCTGTTCTGAAGTTGAAAAAAGAACTCTGTGAAGCACAGTCAGTGAATGAATCTCATATTCCAAATGCGCTCCTGGAAAGATTGGTGACGGATACAAGAGAGTTTCCTCCAGTTTGCCCCATCATTGGAGGGATTCTTGGGCAGGAGGTTATTAAGACAATATCAGAGAAAGGAGACCCTGTCAAGAATTTCTTCTTCTTCGATGCTATGGATGGAAAGGGCGTAATCGAGGACATATCAGGGAACCCTTGA

Protein Analysis

321

Amino Acids

35.97

Weight (kDa)

5.31

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 14 - 310 2.9e-25 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 378
AccB1I GGYRCC 2 cut(s) 65, 71
AccII CGCG 2 cut(s) 209, 315
AcsI RAATTY 2 cut(s) 368, 898
AcuI CTGAAG 1 cut(s) 740
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 3 cut(s) 41, 153, 594
AfiI CCNNNNNNNGG 2 cut(s) 55, 836
AgsI TTSAA 3 cut(s) 444, 662, 727
AhdI GACNNNNNGTC 1 cut(s) 211
AjnI CCWGG 3 cut(s) 611, 682, 783
AluBI AGCT 5 cut(s) 95, 345, 390, 573, 628
AluI AGCT 5 cut(s) 95, 345, 390, 573, 628
Alw26I GTCTC 3 cut(s) 23, 471, 879
ApoI RAATTY 2 cut(s) 368, 898
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
Asp700I GAANNNNTTC 2 cut(s) 439, 902
AspLEI GCGC 2 cut(s) 211, 781
AsuHPI GGTGA 3 cut(s) 344, 500, 808
AsuII TTCGAA 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 76
BaeI ACNNNNGTAYC 2 cut(s) 548, 581
BanI GGYRCC 2 cut(s) 65, 71
BbsI GAAGAC 3 cut(s) 88, 210, 432
BccI CCATC 3 cut(s) 301, 839, 917
BceAI ACGGC 2 cut(s) 22, 264
BciT130I CCWGG 3 cut(s) 613, 684, 785
BciVI GTATCC 2 cut(s) 576, 796
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 3 cut(s) 23, 471, 879
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 3 cut(s) 321, 361, 560
BfuI GTATCC 2 cut(s) 576, 796
BglII AGATCT 1 cut(s) 703
Bme1390I CCNGG 3 cut(s) 613, 684, 785
BmeRI GACNNNNNGTC 1 cut(s) 211
BmiI GGNNCC 3 cut(s) 67, 73, 956
BmrFI CCNGG 3 cut(s) 613, 684, 785
BmsI GCATC 1 cut(s) 904
BpiI GAAGAC 3 cut(s) 88, 210, 432
BpmI CTGGAG 2 cut(s) 705, 804
Bpu14I TTCGAA 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 2 cut(s) 596, 611
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 836
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 3 cut(s) 124, 183, 821
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 3 cut(s) 613, 684, 785
BseDI CCNNGG 2 cut(s) 596, 611
BseGI GGATG 2 cut(s) 190, 928
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 2 cut(s) 55, 836
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 3 cut(s) 124, 183, 821
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 2 cut(s) 209, 315
Bsh1285I CGRYCG 1 cut(s) 46
BshNI GGYRCC 2 cut(s) 65, 71
BsiEI CGRYCG 1 cut(s) 46
BsiSI CCGG 2 cut(s) 69, 159
BslI CCNNNNNNNGG 2 cut(s) 55, 836
BsmAI GTCTC 3 cut(s) 23, 471, 879
Bso31I GGTCTC 1 cut(s) 879
Bsp119I TTCGAA 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 5 cut(s) 43, 187, 417, 502, 703
BspCNI CTCAG 1 cut(s) 13
BspFNI CGCG 2 cut(s) 209, 315
BspLI GGNNCC 3 cut(s) 67, 73, 956
BspT104I TTCGAA 1 cut(s) 524
BspT107I GGYRCC 2 cut(s) 65, 71
BspTNI GGTCTC 1 cut(s) 879
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 3 cut(s) 124, 183, 821
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 596, 611
BssMI GATC 5 cut(s) 43, 187, 417, 502, 703
BssNI GRCGYC 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 596
Bst2UI CCWGG 3 cut(s) 613, 684, 785
Bst4CI ACNGT 4 cut(s) 127, 466, 564, 750
BstACI GRCGYC 1 cut(s) 375
BstBI TTCGAA 1 cut(s) 524
BstDEI CTNAG 2 cut(s) 21, 86
BstF5I GGATG 2 cut(s) 190, 928
BstFNI CGCG 2 cut(s) 209, 315
BstHHI GCGC 2 cut(s) 211, 781
BstKTI GATC 5 cut(s) 46, 190, 420, 505, 706
BstMAI GTCTC 3 cut(s) 23, 471, 879
BstMBI GATC 5 cut(s) 43, 187, 417, 502, 703
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 3 cut(s) 613, 684, 785
BstSCI CCNGG 3 cut(s) 611, 682, 783
BstSFI CTRYAG 3 cut(s) 321, 361, 560
BstSLI GKGCMC 1 cut(s) 76
BstUI CGCG 2 cut(s) 209, 315
BstV2I GAAGAC 3 cut(s) 88, 210, 432
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuI GTATCC 2 cut(s) 576, 796
BtsCI GGATG 2 cut(s) 190, 928
BtsIMutI CAGTG 2 cut(s) 176, 759
CfoI GCGC 2 cut(s) 211, 781
Cfr10I RCCGGY 1 cut(s) 68
CseI GACGC 1 cut(s) 215
Csp6I GTAC 3 cut(s) 40, 152, 593
CviAII CATG 1 cut(s) 98
CviJI RGCY 7 cut(s) 95, 158, 345, 390, 573, 628, 668
CviKI_1 RGCY 7 cut(s) 95, 158, 345, 390, 573, 628, 668
CviQI GTAC 3 cut(s) 40, 152, 593
DdeI CTNAG 2 cut(s) 21, 86
DpnI GATC 5 cut(s) 45, 189, 419, 504, 705
DpnII GATC 5 cut(s) 43, 187, 417, 502, 703
DriI GACNNNNNGTC 1 cut(s) 211
Eam1105I GACNNNNNGTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 879
Eco57I CTGAAG 1 cut(s) 740
EcoRII CCWGG 3 cut(s) 611, 682, 783
EcoT14I CCWWGG 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 596
FaeI CATG 1 cut(s) 101
FalI AAGNNNNNCTT 4 cut(s) 617, 649, 887, 919
FatI CATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 417
FokI GGATG 2 cut(s) 197, 935
FspBI CTAG 1 cut(s) 155
GlaI GCGC 2 cut(s) 210, 780
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 2 cut(s) 705, 804
HapII CCGG 2 cut(s) 69, 159
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 211, 781
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 2 cut(s) 209, 779
HinP1I GCGC 2 cut(s) 209, 779
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 2 cut(s) 343, 571
HinfI GANTC 6 cut(s) 284, 479, 581, 618, 761, 844
HpaII CCGG 2 cut(s) 69, 159
HphI GGTGA 3 cut(s) 344, 500, 808
Hpy166II GTNNAC 3 cut(s) 18, 557, 680
Hpy188I TCNGA 6 cut(s) 228, 238, 291, 306, 720, 877
Hpy188III TCNNGA 1 cut(s) 895
Hpy8I GTNNAC 3 cut(s) 18, 557, 680
HpyAV CCTTC 3 cut(s) 243, 409, 720
HpyCH4III ACNGT 4 cut(s) 127, 466, 564, 750
HpyCH4IV ACGT 2 cut(s) 375, 676
HpyCH4V TGCA 3 cut(s) 131, 508, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 2 cut(s) 21, 86
HpySE526I ACGT 2 cut(s) 375, 676
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 2 cut(s) 209, 779
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 5 cut(s) 43, 187, 417, 502, 703
LmnI GCTCC 2 cut(s) 401, 786
LweI GCATC 1 cut(s) 904
MaeI CTAG 1 cut(s) 155
MaeII ACGT 2 cut(s) 375, 676
MaeIII GTNAC 3 cut(s) 196, 332, 796
MalI GATC 5 cut(s) 45, 189, 419, 504, 705
MboI GATC 5 cut(s) 43, 187, 417, 502, 703
MboII GAAGA 9 cut(s) 93, 215, 432, 538, 674, 698, 895, 898, 901
MfeI CAATTG 1 cut(s) 696
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 5 cut(s) 368, 413, 585, 696, 898
MlyI GAGTC 1 cut(s) 473
MnlI CCTC 7 cut(s) 4, 532, 571, 828, 833, 850, 934
MroXI GAANNNNTTC 2 cut(s) 439, 902
MseI TTAA 3 cut(s) 300, 338, 864
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 2 cut(s) 69, 159
MspR9I CCNGG 3 cut(s) 613, 684, 785
MunI CAATTG 1 cut(s) 696
MvaI CCWGG 3 cut(s) 613, 684, 785
MvnI CGCG 2 cut(s) 209, 315
MwoI GCNNNNNNNGC 1 cut(s) 215
NdeII GATC 5 cut(s) 43, 187, 417, 502, 703
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 3 cut(s) 67, 73, 956
NmeAIII GCCGAG 1 cut(s) 694
NmuCI GTSAC 3 cut(s) 196, 332, 796
NspV TTCGAA 1 cut(s) 524
PdmI GAANNNNTTC 2 cut(s) 439, 902
PfeI GAWTC 5 cut(s) 284, 581, 618, 761, 844
PflFI GACNNNGTC 1 cut(s) 890
PfoI TCCNGGA 1 cut(s) 783
Ple19I CGATCG 1 cut(s) 46
PleI GAGTC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 473
Psp6I CCWGG 3 cut(s) 611, 682, 783
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 3 cut(s) 611, 682, 783
PspN4I GGNNCC 3 cut(s) 67, 73, 956
PsuI RGATCY 1 cut(s) 703
PsyI GACNNNGTC 1 cut(s) 890
PvuI CGATCG 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 390
RsaI GTAC 3 cut(s) 41, 153, 594
RsaNI GTAC 3 cut(s) 40, 152, 593
SaqAI TTAA 3 cut(s) 300, 338, 864
Sau3AI GATC 5 cut(s) 43, 187, 417, 502, 703
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 3 cut(s) 613, 684, 785
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 1 cut(s) 904
SfcI CTRYAG 3 cut(s) 321, 361, 560
SfuI TTCGAA 1 cut(s) 524
Sse9I AATT 5 cut(s) 368, 413, 585, 696, 898
SspI AATATT 2 cut(s) 495, 521
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 3 cut(s) 611, 682, 783
StyI CCWWGG 1 cut(s) 596
TaaI ACNGT 4 cut(s) 127, 466, 564, 750
TaiI ACGT 2 cut(s) 378, 679
TaqI TCGA 4 cut(s) 524, 549, 912, 939
TasI AATT 5 cut(s) 368, 413, 585, 696, 898
TatI WGTACW 1 cut(s) 151
TfiI GAWTC 5 cut(s) 284, 581, 618, 761, 844
Tru1I TTAA 3 cut(s) 300, 338, 864
Tru9I TTAA 3 cut(s) 300, 338, 864
TscAI CASTG 2 cut(s) 183, 759
TseFI GTSAC 3 cut(s) 196, 332, 796
Tsp45I GTSAC 3 cut(s) 196, 332, 796
TspDTI ATGAA 3 cut(s) 255, 438, 774
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 2 cut(s) 183, 759
Tth111I GACNNNGTC 1 cut(s) 890
XapI RAATTY 2 cut(s) 368, 898
XmnI GAANNNNTTC 2 cut(s) 439, 902
XspI CTAG 1 cut(s) 155
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.