Rorug06G0500000

SUMO-activating enzyme subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008631
Physical Location & Seq
Forward (+)
61547649 .. 61549589
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0500000.1

Sequence Viewer

Length: 1533 bp
ATGAAATTGAAACCCACACCCAACCCGGACCCAGACCCAGACCTCTCCTACATCTACTCCTTCCTCCACTCCTCTCTCCACCACCAAATGAACAATGATACCGCCAAGAAACGCCGTCGTAAGACCGCCGCCGCCGCCGACGACGACGGCCCGCCGGGAAAGCAAGGCAAGATGAAGGACCTGAAGGGGATCATCACCACCCTGTCGCTGCTGGAGCAGCAGGAGAAGGAGGACCTCGACCGGGCCTCAAATGACGACAAGTCGTTTGCCGAAGAGAAGCAGAAGCAGACGACGAAGACCAAGGCCATGGTGGAGTACTACTCCGATTTGCAGGGCTACTACTCGGACGTCGAGGAGTCCGAGACCGTGAAGCGCAAGAAGTCGAGAAACGCGGCGACCGCGGCCGTTGCTGCCGCGGCGGCGGCGGCCTCCGAAAAGGGCGCGAAACCCGGAGGAAGCGGGTCGGGTCACCAGCGGAGGCTTTGGGTCAAGGACCGGTCCAAGGCCTGGTGGGACGAGTGTAACCAACCCGATTTCCCGGAAGAAGACTTCAAAAAAGCCTTCCGGATGGGGAAGGCCACGTTCGATTTGATCTGCGACGAGCTCAATTCGGCTATAGCGAAAGAGGACACCACTCTCCGGAATGCAATTCCGGTCCGGCAGAGAGTCGCCGTCTGTTTGTGGAGGTTAGCTACCGGCGACCCGCTCCGGCTCGTTTCGAAGCGATTCGGGTTAGGGATATCGACTTGTCATAAGCTAGTACTCGAAGTCTGCTCAGCGATTAGGACTGTGTTGATGCCCAAGTACTTGAATTGGCCGGAGGATGGTGTTTTGCGGAAGGTGAAGGACGAGTTCGAATCGTTTTCGGGTATACCTAATGTTGTAGGGTCTATGTACACCACTCATATTCCGATCATAGCTCCGAAGATTAGTGTGGCAGCTTATTTTAACAAGAGGCATACAGAGAGGAACCAGAAGACTAGCTACTCCATCACTGTGCAAGGTGTGGTGAATCCGAAAGGGGTGTTCACTGATGTGTGTATAGGGTGGCCTGGTTCAATGCCGGATGATCAGGTTCTGGAGAAGTCGGCTTTGCATCAGAGGGCCGGGAATGGTTTGCTTAAAGGTGTGTGGATTGTGGGGGGTTCGGGGTACCCTTTGCTGGACTGGGTGCTGGTGCCGTATACTCAGCAGCATCTTACTTGGACGCAGCATGCGTTCAATGAGAAGATTGGGGAGATTCAGAATGTGTCGAAAGATGCGTTTGCGAGGTTGAAAGGGAGGTGGTTGTGCTTGCAGAAGAGGACGGAGGTGAAGCTCCAGGACTTGCCTGTGGTTCTTGGGGCTTGCTGTGTGCTGCACAACATATGCGAGCTGAGGAATGAGGAAATCGACCCCGAGCTGAGGTTTGAGCTTGTGGATGATGAGATGGTTCCGGAGGTTGCTTTGAGATCACAAAGCTCAATGAAGGCGAGGGATTCCATTGCTCATAATCTTTTGCATCATGGCCTTGCAGGCACTTCTTTTCTCTAG

Protein Analysis

510

Amino Acids

57.06

Weight (kDa)

8.61

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 299 - 455 1.1e-34 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 1152
AccB1I GGYRCC 2 cut(s) 1152, 1177
AccB7I CCANNNNNTGG 1 cut(s) 507
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 2 cut(s) 871, 1184
AccII CGCG 4 cut(s) 392, 401, 416, 443
AccIII TCCGGA 3 cut(s) 564, 639, 1435
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 2 cut(s) 402, 815
AcuI CTGAAG 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 5 cut(s) 317, 762, 806, 896, 1154
AfiI CCNNNNNNNGG 7 cut(s) 241, 502, 507, 639, 824, 1162, 1404
AgeI ACCGGT 1 cut(s) 495
AgsI TTSAA 6 cut(s) 10, 553, 811, 1059, 1222, 1276
AhdI GACNNNNNGTC 1 cut(s) 259
AjnI CCWGG 3 cut(s) 506, 1051, 1320
AleI CACNNNNGTG 1 cut(s) 1034
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 1078
Ama87I CYCGRG 1 cut(s) 1397
Aor13HI TCCGGA 3 cut(s) 564, 639, 1435
ApeKI GCWGC 7 cut(s) 208, 217, 410, 938, 1192, 1210, 1357
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AsiGI ACCGGT 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 725
Asp718I GGTACC 1 cut(s) 1152
AspLEI GCGC 2 cut(s) 375, 443
AspS9I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 493, 498, 655, 1104
AsuC2I CCSGG 6 cut(s) 26, 156, 242, 450, 539, 1108
AsuHPI GGTGA 5 cut(s) 187, 461, 853, 1021, 1324
AsuII TTCGAA 2 cut(s) 719, 855
AvaI CYCGRG 1 cut(s) 1397
AvaII GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
BanI GGYRCC 2 cut(s) 1152, 1177
BanII GRGCYC 1 cut(s) 606
BarI GAAGNNNNNNTAC 2 cut(s) 968, 1000
BbsI GAAGAC 3 cut(s) 302, 552, 983
Bbv12I GWGCWC 1 cut(s) 606
BbvCI CCTCAGC 2 cut(s) 1376, 1403
BbvI GCAGC 7 cut(s) 195, 229, 397, 950, 1204, 1222, 1344
BccI CCATC 4 cut(s) 562, 818, 998, 1423
BceAI ACGGC 5 cut(s) 99, 163, 389, 656, 1165
BciT130I CCWGG 3 cut(s) 508, 1053, 1322
BclI TGATCA 1 cut(s) 1069
BcnI CCSGG 6 cut(s) 26, 156, 242, 450, 539, 1108
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 3 cut(s) 758, 981, 1531
BfmI CTRYAG 1 cut(s) 615
BglI GCCNNNNNGGC 2 cut(s) 419, 1515
BlpI GCTNAGC 1 cut(s) 775
BmcAI AGTACT 3 cut(s) 317, 762, 806
Bme1390I CCNGG 9 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1108, 1322
Bme18I GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
BmeRI GACNNNNNGTC 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 493, 498, 655, 1104
BmiI GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
BmrFI CCNGG 9 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1108, 1322
BmrI ACTGGG 1 cut(s) 1177
BmsI GCATC 5 cut(s) 786, 1105, 1204, 1249, 1510
BmuI ACTGGG 1 cut(s) 1177
BpiI GAAGAC 3 cut(s) 302, 552, 983
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 3 cut(s) 233, 1100, 1304
Bpu10I CCTNAGC 2 cut(s) 1376, 1403
Bpu1102I GCTNAGC 1 cut(s) 775
Bpu14I TTCGAA 2 cut(s) 719, 855
BpuMI CCSGG 6 cut(s) 26, 156, 242, 450, 539, 1108
BsaHI GRCGYC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 5 cut(s) 300, 306, 399, 414, 501
BsaWI WCCGGW 5 cut(s) 495, 564, 639, 652, 1435
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 7 cut(s) 241, 502, 507, 639, 824, 1162, 1404
Bse118I RCCGGY 2 cut(s) 495, 695
Bse1I ACTGG 1 cut(s) 1172
Bse3DI GCAATG 1 cut(s) 1482
BseAI TCCGGA 3 cut(s) 564, 639, 1435
BseBI CCWGG 3 cut(s) 508, 1053, 1322
BseDI CCNNGG 5 cut(s) 300, 306, 399, 414, 501
BseGI GGATG 4 cut(s) 573, 829, 1072, 1426
BseLI CCNNNNNNNGG 7 cut(s) 241, 502, 507, 639, 824, 1162, 1404
BseMI GCAATG 1 cut(s) 1482
BseMII CTCAG 4 cut(s) 789, 1202, 1367, 1394
BseNI ACTGG 1 cut(s) 1172
BseRI GAGGAG 2 cut(s) 61, 368
BseX3I CGGCCG 1 cut(s) 402
BseXI GCAGC 7 cut(s) 195, 229, 397, 950, 1204, 1222, 1344
BsgI GTGCAG 1 cut(s) 1343
Bsh1236I CGCG 4 cut(s) 392, 401, 416, 443
Bsh1285I CGRYCG 3 cut(s) 241, 399, 405
BshNI GGYRCC 2 cut(s) 1152, 1177
BshTI ACCGGT 1 cut(s) 495
BsiEI CGRYCG 3 cut(s) 241, 399, 405
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 1 cut(s) 1397
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 7 cut(s) 241, 502, 507, 639, 824, 1162, 1404
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 649
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 1397
Bsp119I TTCGAA 2 cut(s) 719, 855
Bsp1286I GDGCHC 1 cut(s) 606
Bsp13I TCCGGA 3 cut(s) 564, 639, 1435
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 5 cut(s) 189, 591, 912, 1069, 1451
Bsp1720I GCTNAGC 1 cut(s) 775
Bsp19I CCATGG 1 cut(s) 306
BspCNI CTCAG 4 cut(s) 788, 1201, 1368, 1395
BspEI TCCGGA 3 cut(s) 564, 639, 1435
BspFNI CGCG 4 cut(s) 392, 401, 416, 443
BspLI GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
BspPI GGATC 1 cut(s) 197
BspT104I TTCGAA 2 cut(s) 719, 855
BspT107I GGYRCC 2 cut(s) 1152, 1177
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 1482
BsrFI RCCGGY 2 cut(s) 495, 695
BsrGI TGTACA 1 cut(s) 894
BsrI ACTGG 1 cut(s) 1172
BssAI RCCGGY 2 cut(s) 495, 695
BssECI CCNNGG 5 cut(s) 300, 306, 399, 414, 501
BssMI GATC 5 cut(s) 189, 591, 912, 1069, 1451
BssNAI GTATAC 2 cut(s) 872, 1185
BssNI GRCGYC 1 cut(s) 348
BssT1I CCWWGG 3 cut(s) 300, 306, 501
Bst1107I GTATAC 2 cut(s) 872, 1185
Bst2UI CCWGG 3 cut(s) 508, 1053, 1322
Bst4CI ACNGT 3 cut(s) 367, 790, 997
Bst6I CTCTTC 2 cut(s) 267, 1295
BstACI GRCGYC 1 cut(s) 348
BstAUI TGTACA 1 cut(s) 894
BstBI TTCGAA 2 cut(s) 719, 855
BstC8I GCNNGC 6 cut(s) 152, 1215, 1295, 1348, 1373, 1516
BstDEI CTNAG 4 cut(s) 775, 1188, 1376, 1403
BstDSI CCRYGG 3 cut(s) 306, 399, 414
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 4 cut(s) 573, 829, 1072, 1426
BstFNI CGCG 4 cut(s) 392, 401, 416, 443
BstHHI GCGC 2 cut(s) 375, 443
BstKTI GATC 5 cut(s) 192, 594, 915, 1072, 1454
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 5 cut(s) 189, 591, 912, 1069, 1451
BstMCI CGRYCG 3 cut(s) 241, 399, 405
BstNI CCWGG 3 cut(s) 508, 1053, 1322
BstNSI RCATGY 1 cut(s) 1217
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 9 cut(s) 24, 154, 240, 448, 506, 537, 1051, 1106, 1320
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 4 cut(s) 392, 401, 416, 443
BstV1I GCAGC 7 cut(s) 195, 229, 397, 950, 1204, 1222, 1344
BstV2I GAAGAC 3 cut(s) 302, 552, 983
BstXI CCANNNNNNTGG 1 cut(s) 307
BstZ17I GTATAC 2 cut(s) 872, 1185
BstZI CGGCCG 1 cut(s) 402
BtgI CCRYGG 3 cut(s) 306, 399, 414
BtsCI GGATG 4 cut(s) 573, 829, 1072, 1426
BtsIMutI CAGTG 2 cut(s) 993, 1029
Cac8I GCNNGC 6 cut(s) 152, 1215, 1295, 1348, 1373, 1516
CaiI CAGNNNCTG 1 cut(s) 1078
CfoI GCGC 2 cut(s) 375, 443
Cfr10I RCCGGY 2 cut(s) 495, 695
Cfr13I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 493, 498, 655, 1104
Cfr42I CCGCGG 2 cut(s) 402, 417
CpoI CGGWCCG 1 cut(s) 655
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 5 cut(s) 316, 761, 805, 895, 1153
CspAI ACCGGT 1 cut(s) 495
CspI CGGWCCG 1 cut(s) 655
CviAII CATG 3 cut(s) 307, 1214, 1505
CviQI GTAC 5 cut(s) 316, 761, 805, 895, 1153
DdeI CTNAG 4 cut(s) 775, 1188, 1376, 1403
DpnI GATC 5 cut(s) 191, 593, 914, 1071, 1453
DpnII GATC 5 cut(s) 189, 591, 912, 1069, 1451
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 2 cut(s) 402, 815
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 267, 1295
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 2 cut(s) 267, 1295
Ecl136II GAGCTC 1 cut(s) 604
EclXI CGGCCG 1 cut(s) 402
Eco130I CCWWGG 3 cut(s) 300, 306, 501
Eco147I AGGCCT 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 606
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 741
Eco47I GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
Eco52I CGGCCG 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 203
Eco88I CYCGRG 1 cut(s) 1397
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 604
EcoO109I RGGNCCY 2 cut(s) 178, 232
EcoO65I GGTNACC 1 cut(s) 467
EcoRII CCWGG 3 cut(s) 506, 1051, 1320
EcoRV GATATC 1 cut(s) 741
EcoT14I CCWWGG 3 cut(s) 300, 306, 501
EcoT38I GRGCYC 1 cut(s) 606
ErhI CCWWGG 3 cut(s) 300, 306, 501
FaeI CATG 3 cut(s) 310, 1217, 1508
FaqI GGGAC 1 cut(s) 527
FatI CATG 3 cut(s) 306, 1213, 1504
FauI CCCGC 3 cut(s) 159, 452, 711
FauNDI CATATG 1 cut(s) 1367
FbaI TGATCA 1 cut(s) 1069
FblI GTMKAC 2 cut(s) 871, 1184
FokI GGATG 4 cut(s) 580, 836, 1079, 1433
FriOI GRGCYC 1 cut(s) 606
FspBI CTAG 3 cut(s) 758, 981, 1531
GlaI GCGC 2 cut(s) 374, 442
GsuI CTGGAG 3 cut(s) 233, 1100, 1304
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 2 cut(s) 375, 443
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 3 cut(s) 310, 1217, 1508
Hin6I GCGC 2 cut(s) 373, 441
HinP1I GCGC 2 cut(s) 373, 441
HinfI GANTC 7 cut(s) 356, 666, 726, 857, 1012, 1240, 1478
HphI GGTGA 5 cut(s) 187, 461, 853, 1021, 1324
Hpy166II GTNNAC 4 cut(s) 872, 897, 1029, 1185
Hpy188I TCNGA 9 cut(s) 325, 346, 361, 433, 912, 924, 1017, 1101, 1245
Hpy188III TCNNGA 5 cut(s) 384, 565, 640, 1079, 1436
Hpy8I GTNNAC 4 cut(s) 872, 897, 1029, 1185
Hpy99I CGWCG 7 cut(s) 120, 143, 146, 149, 295, 353, 602
HpyAV CCTTC 9 cut(s) 70, 169, 178, 220, 568, 571, 832, 838, 1462
HpyCH4III ACNGT 3 cut(s) 367, 790, 997
HpyCH4IV ACGT 2 cut(s) 348, 581
HpyCH4V TGCA 8 cut(s) 331, 647, 1000, 1096, 1297, 1360, 1501, 1514
HpyF3I CTNAG 4 cut(s) 775, 1188, 1376, 1403
HpySE526I ACGT 2 cut(s) 348, 581
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 3 cut(s) 310, 1217, 1508
HspAI GCGC 2 cut(s) 373, 441
Kpn2I TCCGGA 3 cut(s) 564, 639, 1435
KpnI GGTACC 1 cut(s) 1156
Ksp22I TGATCA 1 cut(s) 1069
KspI CCGCGG 2 cut(s) 402, 417
Kzo9I GATC 5 cut(s) 189, 591, 912, 1069, 1451
LmnI GCTCC 4 cut(s) 214, 711, 925, 1323
Lsp1109I GCAGC 7 cut(s) 195, 229, 397, 950, 1204, 1222, 1344
LweI GCATC 5 cut(s) 786, 1105, 1204, 1249, 1510
MaeI CTAG 3 cut(s) 758, 981, 1531
MaeII ACGT 2 cut(s) 348, 581
MaeIII GTNAC 2 cut(s) 467, 521
MalI GATC 5 cut(s) 191, 593, 914, 1071, 1453
MbiI CCGCTC 1 cut(s) 706
MboI GATC 5 cut(s) 189, 591, 912, 1069, 1451
MboII GAAGA 8 cut(s) 284, 307, 554, 557, 937, 988, 1240, 1312
MhlI GDGCHC 1 cut(s) 606
MluCI AATT 4 cut(s) 5, 607, 648, 811
MlyI GAGTC 2 cut(s) 365, 675
MroI TCCGGA 3 cut(s) 564, 639, 1435
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 2 cut(s) 948, 1122
MslI CAYNNNNRTG 2 cut(s) 995, 1034
MspA1I CMGCKG 3 cut(s) 401, 416, 475
MspR9I CCNGG 9 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1108, 1322
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 3 cut(s) 508, 1053, 1322
MvnI CGCG 4 cut(s) 392, 401, 416, 443
NciI CCSGG 6 cut(s) 26, 156, 242, 450, 539, 1108
NcoI CCATGG 1 cut(s) 306
NdeI CATATG 1 cut(s) 1367
NdeII GATC 5 cut(s) 189, 591, 912, 1069, 1451
NlaIII CATG 3 cut(s) 310, 1217, 1508
NlaIV GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 1 cut(s) 1217
NspV TTCGAA 2 cut(s) 719, 855
OliI CACNNNNGTG 1 cut(s) 1034
PaeI GCATGC 1 cut(s) 1217
PceI AGGCCT 1 cut(s) 506
PcsI WCGNNNNNNNCGW 4 cut(s) 357, 617, 1214, 1259
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 5 cut(s) 726, 857, 1012, 1240, 1478
PflMI CCANNNNNTGG 1 cut(s) 507
PfoI TCCNGGA 2 cut(s) 537, 1320
PinAI ACCGGT 1 cut(s) 495
PleI GAGTC 2 cut(s) 364, 674
PpsI GAGTC 2 cut(s) 364, 674
PpuMI RGGWCCY 2 cut(s) 178, 232
Psp124BI GAGCTC 1 cut(s) 606
Psp5II RGGWCCY 2 cut(s) 178, 232
Psp6I CCWGG 3 cut(s) 506, 1051, 1320
PspEI GGTNACC 1 cut(s) 467
PspGI CCWGG 3 cut(s) 506, 1051, 1320
PspN4I GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
PspPI GGNCC 9 cut(s) 28, 149, 178, 232, 243, 493, 498, 655, 1104
PspPPI RGGWCCY 2 cut(s) 178, 232
PstNI CAGNNNCTG 1 cut(s) 1078
RsaI GTAC 5 cut(s) 317, 762, 806, 896, 1154
RsaNI GTAC 5 cut(s) 316, 761, 805, 895, 1153
RseI CAYNNNNRTG 2 cut(s) 995, 1034
Rsr2I CGGWCCG 1 cut(s) 655
RsrII CGGWCCG 1 cut(s) 655
SacI GAGCTC 1 cut(s) 606
SacII CCGCGG 2 cut(s) 402, 417
SaqAI TTAA 2 cut(s) 948, 1122
Sau3AI GATC 5 cut(s) 189, 591, 912, 1069, 1451
Sau96I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 493, 498, 655, 1104
ScaI AGTACT 3 cut(s) 317, 762, 806
SchI GAGTC 2 cut(s) 365, 675
ScrFI CCNGG 9 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1108, 1322
SduI GDGCHC 1 cut(s) 606
SfaNI GCATC 5 cut(s) 786, 1105, 1204, 1249, 1510
SfcI CTRYAG 1 cut(s) 615
Sfr303I CCGCGG 2 cut(s) 402, 417
SfuI TTCGAA 2 cut(s) 719, 855
SgrBI CCGCGG 2 cut(s) 402, 417
SinI GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
SmiMI CAYNNNNRTG 2 cut(s) 995, 1034
SphI GCATGC 1 cut(s) 1217
Sse9I AATT 4 cut(s) 5, 607, 648, 811
SseBI AGGCCT 1 cut(s) 506
SspMI CTAG 3 cut(s) 758, 981, 1531
SstI GAGCTC 1 cut(s) 606
StuI AGGCCT 1 cut(s) 506
StyD4I CCNGG 9 cut(s) 24, 154, 240, 448, 506, 537, 1051, 1106, 1320
StyI CCWWGG 3 cut(s) 300, 306, 501
TaaI ACNGT 3 cut(s) 367, 790, 997
TaiI ACGT 2 cut(s) 351, 584
TasI AATT 4 cut(s) 5, 607, 648, 811
TatI WGTACW 4 cut(s) 315, 760, 804, 894
TfiI GAWTC 5 cut(s) 726, 857, 1012, 1240, 1478
Tru1I TTAA 2 cut(s) 948, 1122
Tru9I TTAA 2 cut(s) 948, 1122
TscAI CASTG 2 cut(s) 1000, 1036
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 7 cut(s) 208, 217, 410, 938, 1192, 1210, 1357
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 4 cut(s) 17, 104, 188, 1481
TspGWI ACGGA 1 cut(s) 1322
TspRI CASTG 2 cut(s) 1000, 1036
Van91I CCANNNNNTGG 1 cut(s) 507
VpaK11BI GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
XceI RCATGY 1 cut(s) 1217
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmiI GTMKAC 2 cut(s) 871, 1184
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 3 cut(s) 758, 981, 1531
ZraI GACGTC 1 cut(s) 349
ZrmI AGTACT 3 cut(s) 317, 762, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.