AT5G50970

WD-40 repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007591
Physical Location & Seq
Forward (+)
20736227 .. 20739358
3132 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50970.1

Sequence Viewer

Length: 1539 bp
ATGAGCGAGCTTTGTACGAATCCTAAGAAACCCAAACTCAAAGAAGAAGATGAAGAAGAAGAAGCTAATGTCTCTTGCAGAGAAGAGCAAGAAGAAGTTCTTGTTGCTTTAGTAGAGCACCGTTCTAATGAAATTGAGCGTCTCAACAATCACATATCTAATTACCAAACCAAGCTTATTGAAGCAGAGAGAAGTTTGCAGAATTCAAAAGCTAAGCTAGCTCAACTTCGTGGAGTTGCAGTTCCATCTATCAGTGGTGCAAAGAAAGATCATAAACCATTGAAGACTTTGAGAAATGTGAATGTGAGTGAAGACTATGCATCTCCATCACCTAGCAAGACTCTAAGGCCCTCTGATAGCTCTGATCATCCTAGAAGTAGTTGTGGTAGTAGTGTCTCCAAGGCTAAGACTGTTGTTGTGAAGCAAAAATCTGAAACATCTCGTGATAGTCCTAATGTTAAAGATAGAGGGATCAAGAGGAAATTTGAGCAAAAAGAGCATAAAGAGTTGATTCGGTTGATAGCGAGAAACTCTTCACCAACTACAATCAAGTGTCATACTAGTAACCAAATCTCTAGTCAGCATAAGAGGAAATTAAGAAGCTTGATATTGTGTCCTGTAAATGAGCAGTTGTTTGCAACAAGTTCTTTGGATGGCATGGTTAGTTTATGGCAACTGCAGCCTGGAAGATTGGTTGCATCATTGCTTAGTACTACCGACTGTTTATCTCGAAAACAAAGAAGATGGGCAGAAGATATGGCGTGGCACCCATCTGGGAACACTCTTTTCTCTGTGTACACTGCAGACGATGGGGATTCTCAGATATCGATCCTAAATCTGAACAAGACACGCGAGGTTACTTTCCTGGAAAATAAACCTCACGTAAAAGGGATTATCAACAACATAAAGTTCATGCCTTGGGAAAATACATGCTTTGTCACAGGGGGAAGTGATCACGCTGTTGTACTATGGAACGAGAGTGATGATGAAGAAAACAAATGGAAGTCGAAACCTTTACACAGAAATCTACATTCAGCTGCTGTAATGGGAGTGGATGGGATGAAAAACAAGAATGTTATTTTATCAGTAGGAGCAGACAAGAGAATATACGGATTTGATGTTCAAGTTGGTAGAGCAGACTACAAGCATCAAATAGACTATAAATGCATGAGCGTCCTCGCTAATCCTTGTGACTTCAATCTTTTCATGGTTCAATCCGGGGAACCCGAGAAACAGCTTCGACTGTTTGATATCAGGTTAAGAAAAACCGAACTCCATTCGTTTGGGTGGAAGCAAGACAGCAGTGAATCACAATCAGCTCTAATAAACCAGTCTTGGTCTCCTGATGGTTTATACATCACCTCTGGCTCAGTTGATCCGGTGATCCATGTTTTTGACATCAGGTACAATGCTCGTAAGCCGACACAATCAATAAAAGCGCATCAGAAACGAGTGTTTAAAGCGGAATGGCATTACTCTCAACCACTTCTCATCTCCATATCTTCTGATCTCAACATTGGTCTGCACAAGATCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

512

Amino Acids

57.98

Weight (kDa)

9.1

Isoelectric Point (pI)

64.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 172 - 328 1.2e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 190 - 374 1.9e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 191 - 373 7.4e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 194 - 379 1.7e-10 WDR5 beta-propeller domain
WD40_Prp19 PF24814 272 - 469 4.4e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 320 - 497 2.4e-08 CDC20/Fizzy WD40 domain
EIF3I PF24805 344 - 507 1.9e-06 EIF3I
Beta-prop_EML_2 PF23414 349 - 509 1.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 440 - 509 2e-07 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 445 - 509 5.8e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 765
AccII CGCG 1 cut(s) 852
AciI CCGC 1 cut(s) 1463
AclWI GGATC 4 cut(s) 479, 823, 1370, 1378
AcsI RAATTY 2 cut(s) 202, 482
AfaI GTAC 5 cut(s) 16, 712, 797, 966, 1406
AgsI TTSAA 6 cut(s) 182, 207, 283, 1124, 1198, 1214
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 2 cut(s) 682, 864
AloI GAACNNNNNNTCC 4 cut(s) 225, 257, 1104, 1136
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 4 cut(s) 76, 146, 400, 1344
AlwI GGATC 4 cut(s) 479, 823, 1370, 1378
AlwNI CAGNNNCTG 1 cut(s) 1040
Ama87I CYCGRG 1 cut(s) 1226
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 679, 1037
ApoI RAATTY 2 cut(s) 202, 482
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
Asp700I GAANNNNTTC 2 cut(s) 96, 532
AspLEI GCGC 1 cut(s) 1441
AspS9I GGNCC 1 cut(s) 348
AsuC2I CCSGG 1 cut(s) 1219
AsuHPI GGTGA 4 cut(s) 321, 528, 1351, 1393
AsuNHI GCTAGC 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 1226
BanI GGYRCC 1 cut(s) 765
BauI CACGAG 1 cut(s) 441
BbsI GAAGAC 2 cut(s) 290, 318
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 691, 1024
BccI CCATC 8 cut(s) 253, 334, 647, 738, 778, 803, 1049, 1340
BciT130I CCWGG 2 cut(s) 684, 866
BclI TGATCA 2 cut(s) 364, 952
BcnI CCSGG 1 cut(s) 1219
BcoDI GTCTC 4 cut(s) 76, 146, 400, 1344
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 5 cut(s) 218, 333, 372, 561, 576
BfmI CTRYAG 2 cut(s) 677, 801
BglII AGATCT 1 cut(s) 1530
BisI GCNGC 2 cut(s) 680, 1038
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 2 cut(s) 681, 1039
BmcAI AGTACT 1 cut(s) 712
Bme1390I CCNGG 3 cut(s) 684, 866, 1219
BmeT110I CYCGRG 1 cut(s) 1226
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 2 cut(s) 767, 1224
BmrFI CCNGG 3 cut(s) 684, 866, 1219
BmsI GCATC 4 cut(s) 329, 707, 1156, 1450
BmtI GCTAGC 1 cut(s) 221
BpiI GAAGAC 2 cut(s) 290, 318
Bpu1102I GCTNAGC 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 1219
Bsa29I ATCGAT 1 cut(s) 827
BsaAI YACGTR 1 cut(s) 883
BsaBI GATNNNNATC 1 cut(s) 827
BsaI GGTCTC 1 cut(s) 1344
BsaJI CCNNGG 3 cut(s) 399, 917, 1218
BsaWI WCCGGW 1 cut(s) 1378
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bse1I ACTGG 1 cut(s) 1330
Bse3DI GCAATG 1 cut(s) 701
Bse8I GATNNNNATC 1 cut(s) 827
BseBI CCWGG 2 cut(s) 684, 866
BseCI ATCGAT 1 cut(s) 827
BseDI CCNNGG 3 cut(s) 399, 917, 1218
BseGI GGATG 4 cut(s) 367, 658, 1060, 1065
BseJI GATNNNNATC 1 cut(s) 827
BseMI GCAATG 1 cut(s) 701
BseMII CTCAG 2 cut(s) 833, 1383
BseNI ACTGG 1 cut(s) 1330
BseXI GCAGC 2 cut(s) 691, 1024
BsgI GTGCAG 1 cut(s) 1508
Bsh1236I CGCG 1 cut(s) 852
BshFI GGCC 1 cut(s) 349
BshNI GGYRCC 1 cut(s) 765
BshVI ATCGAT 1 cut(s) 827
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 1226
BsiSI CCGG 2 cut(s) 1218, 1379
BsmAI GTCTC 4 cut(s) 76, 146, 400, 1344
BsmBI CGTCTC 1 cut(s) 146
BsnI GGCC 1 cut(s) 349
Bso31I GGTCTC 1 cut(s) 1344
BsoBI CYCGRG 1 cut(s) 1226
Bsp1286I GDGCHC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
Bsp1720I GCTNAGC 1 cut(s) 213
BspACI CCGC 1 cut(s) 1463
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 2 cut(s) 832, 1382
BspDI ATCGAT 1 cut(s) 827
BspFNI CGCG 1 cut(s) 852
BspHI TCATGA 1 cut(s) 1535
BspLI GGNNCC 2 cut(s) 767, 1224
BspMAI CTGCAG 2 cut(s) 681, 805
BspOI GCTAGC 1 cut(s) 221
BspPI GGATC 4 cut(s) 479, 823, 1370, 1378
BspQI GCTCTTC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 765
BspTNI GGTCTC 1 cut(s) 1344
BsrDI GCAATG 1 cut(s) 701
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 1 cut(s) 1330
BssECI CCNNGG 3 cut(s) 399, 917, 1218
BssMI GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 2 cut(s) 399, 917
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 684, 866
Bst4CI ACNGT 4 cut(s) 122, 412, 722, 1245
Bst6I CTCTTC 2 cut(s) 78, 538
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 1 cut(s) 883
BstC8I GCNNGC 2 cut(s) 8, 219
BstDEI CTNAG 7 cut(s) 24, 213, 344, 405, 707, 819, 1369
BstF5I GGATG 4 cut(s) 367, 658, 1060, 1065
BstFNI CGCG 1 cut(s) 852
BstHHI GCGC 1 cut(s) 1441
BstKTI GATC 9 cut(s) 271, 367, 474, 831, 955, 1378, 1386, 1510, 1533
BstMAI GTCTC 4 cut(s) 76, 146, 400, 1344
BstMBI GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
BstMWI GCNNNNNNNGC 3 cut(s) 218, 496, 679
BstNI CCWGG 2 cut(s) 684, 866
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 3 cut(s) 682, 864, 1217
BstSFI CTRYAG 2 cut(s) 677, 801
BstUI CGCG 1 cut(s) 852
BstV1I GCAGC 2 cut(s) 691, 1024
BstV2I GAAGAC 2 cut(s) 290, 318
BstX2I RGATCY 1 cut(s) 1530
BstXI CCANNNNNNTGG 1 cut(s) 1283
BstYI RGATCY 1 cut(s) 1530
Bsu15I ATCGAT 1 cut(s) 827
BsuRI GGCC 1 cut(s) 349
BsuTUI ATCGAT 1 cut(s) 827
BtsCI GGATG 4 cut(s) 367, 658, 1060, 1065
BtsI GCAGTG 2 cut(s) 798, 1309
BtsIMutI CAGTG 3 cut(s) 259, 798, 1309
Cac8I GCNNGC 2 cut(s) 8, 219
CaiI CAGNNNCTG 1 cut(s) 1040
CciI TCATGA 1 cut(s) 1535
CfoI GCGC 1 cut(s) 1441
Cfr13I GGNCC 1 cut(s) 348
ClaI ATCGAT 1 cut(s) 827
CseI GACGC 2 cut(s) 128, 1162
Csp6I GTAC 5 cut(s) 15, 711, 796, 965, 1405
CviAII CATG 7 cut(s) 658, 913, 930, 1168, 1207, 1388, 1536
CviQI GTAC 5 cut(s) 15, 711, 796, 965, 1405
DdeI CTNAG 7 cut(s) 24, 213, 344, 405, 707, 819, 1369
DpnI GATC 9 cut(s) 270, 366, 473, 830, 954, 1377, 1385, 1509, 1532
DpnII GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
DraI TTTAAA 1 cut(s) 1459
Eam1104I CTCTTC 2 cut(s) 78, 538
EarI CTCTTC 2 cut(s) 78, 538
Eco130I CCWWGG 2 cut(s) 399, 917
Eco31I GGTCTC 1 cut(s) 1344
Eco32I GATATC 2 cut(s) 825, 1252
Eco88I CYCGRG 1 cut(s) 1226
EcoO109I RGGNCCY 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 682, 864
EcoRV GATATC 2 cut(s) 825, 1252
EcoT14I CCWWGG 2 cut(s) 399, 917
EcoT22I ATGCAT 2 cut(s) 322, 1169
ErhI CCWWGG 2 cut(s) 399, 917
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 7 cut(s) 661, 916, 933, 1171, 1210, 1391, 1539
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 7 cut(s) 657, 912, 929, 1167, 1206, 1387, 1535
FbaI TGATCA 2 cut(s) 364, 952
Fnu4HI GCNGC 2 cut(s) 680, 1038
FokI GGATG 4 cut(s) 354, 665, 1067, 1072
Fsp4HI GCNGC 2 cut(s) 680, 1038
FspBI CTAG 5 cut(s) 218, 333, 372, 561, 576
GlaI GCGC 1 cut(s) 1440
GluI GCNGC 2 cut(s) 680, 1038
HaeIII GGCC 1 cut(s) 349
HapII CCGG 2 cut(s) 1218, 1379
HgaI GACGC 2 cut(s) 128, 1162
HhaI GCGC 1 cut(s) 1441
Hin1II CATG 7 cut(s) 661, 916, 933, 1171, 1210, 1391, 1539
Hin6I GCGC 1 cut(s) 1439
HinP1I GCGC 1 cut(s) 1439
HindIII AAGCTT 2 cut(s) 173, 601
HinfI GANTC 5 cut(s) 19, 340, 511, 815, 1307
HpaII CCGG 2 cut(s) 1218, 1379
HphI GGTGA 4 cut(s) 321, 528, 1351, 1393
Hpy166II GTNNAC 2 cut(s) 796, 798
Hpy188I TCNGA 7 cut(s) 355, 364, 433, 822, 840, 1446, 1507
Hpy188III TCNNGA 5 cut(s) 443, 475, 729, 1343, 1536
Hpy8I GTNNAC 2 cut(s) 796, 798
HpyCH4III ACNGT 4 cut(s) 122, 412, 722, 1245
HpyCH4IV ACGT 1 cut(s) 882
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 496, 679
HpyF3I CTNAG 7 cut(s) 24, 213, 344, 405, 707, 819, 1369
HpySE526I ACGT 1 cut(s) 882
Hsp92II CATG 7 cut(s) 661, 916, 933, 1171, 1210, 1391, 1539
HspAI GCGC 1 cut(s) 1439
Ksp22I TGATCA 2 cut(s) 364, 952
Kzo9I GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
LguI GCTCTTC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 1091
Lsp1109I GCAGC 2 cut(s) 691, 1024
LweI GCATC 4 cut(s) 329, 707, 1156, 1450
MaeI CTAG 5 cut(s) 218, 333, 372, 561, 576
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 4 cut(s) 563, 856, 937, 1190
MalI GATC 9 cut(s) 270, 366, 473, 830, 954, 1377, 1385, 1509, 1532
MboI GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
MflI RGATCY 1 cut(s) 1530
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 5 cut(s) 132, 160, 202, 482, 593
MlyI GAGTC 1 cut(s) 334
MnlI CCTC 8 cut(s) 361, 461, 471, 582, 847, 888, 1187, 1372
Mph1103I ATGCAT 2 cut(s) 322, 1169
MroXI GAANNNNTTC 2 cut(s) 96, 532
MseI TTAA 4 cut(s) 459, 596, 1259, 1458
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 2 cut(s) 1218, 1379
MspR9I CCNGG 3 cut(s) 684, 866, 1219
MvaI CCWGG 2 cut(s) 684, 866
MvnI CGCG 1 cut(s) 852
MwoI GCNNNNNNNGC 3 cut(s) 218, 496, 679
NciI CCSGG 1 cut(s) 1219
NdeII GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
NheI GCTAGC 1 cut(s) 217
NlaIII CATG 7 cut(s) 661, 916, 933, 1171, 1210, 1391, 1539
NlaIV GGNNCC 2 cut(s) 767, 1224
NmuCI GTSAC 2 cut(s) 937, 1190
NsiI ATGCAT 2 cut(s) 322, 1169
NspI RCATGY 1 cut(s) 933
PagI TCATGA 1 cut(s) 1535
PciSI GCTCTTC 1 cut(s) 78
PdmI GAANNNNTTC 2 cut(s) 96, 532
PfeI GAWTC 4 cut(s) 19, 511, 815, 1307
PfoI TCCNGGA 1 cut(s) 864
PkrI GCNGC 2 cut(s) 681, 1039
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
Ppu21I YACGTR 1 cut(s) 883
Psp6I CCWGG 2 cut(s) 682, 864
PspGI CCWGG 2 cut(s) 682, 864
PspN4I GGNNCC 2 cut(s) 767, 1224
PspPI GGNCC 1 cut(s) 348
PstI CTGCAG 2 cut(s) 681, 805
PstNI CAGNNNCTG 1 cut(s) 1040
PsuI RGATCY 1 cut(s) 1530
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 5 cut(s) 16, 712, 797, 966, 1406
RsaNI GTAC 5 cut(s) 15, 711, 796, 965, 1405
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 4 cut(s) 459, 596, 1259, 1458
SatI GCNGC 2 cut(s) 680, 1038
Sau3AI GATC 9 cut(s) 268, 364, 471, 828, 952, 1375, 1383, 1507, 1530
Sau96I GGNCC 1 cut(s) 348
ScaI AGTACT 1 cut(s) 712
SchI GAGTC 1 cut(s) 334
ScrFI CCNGG 3 cut(s) 684, 866, 1219
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 4 cut(s) 329, 707, 1156, 1450
SfcI CTRYAG 2 cut(s) 677, 801
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 5 cut(s) 132, 160, 202, 482, 593
SsiI CCGC 1 cut(s) 1463
SspMI CTAG 5 cut(s) 218, 333, 372, 561, 576
StyD4I CCNGG 3 cut(s) 682, 864, 1217
StyI CCWWGG 2 cut(s) 399, 917
TaaI ACNGT 4 cut(s) 122, 412, 722, 1245
TaiI ACGT 1 cut(s) 885
TaqI TCGA 4 cut(s) 730, 827, 1007, 1240
TasI AATT 5 cut(s) 132, 160, 202, 482, 593
TatI WGTACW 3 cut(s) 710, 795, 964
TfiI GAWTC 4 cut(s) 19, 511, 815, 1307
Tru1I TTAA 4 cut(s) 459, 596, 1259, 1458
Tru9I TTAA 4 cut(s) 459, 596, 1259, 1458
TscAI CASTG 3 cut(s) 259, 805, 1309
TseFI GTSAC 2 cut(s) 937, 1190
TseI GCWGC 2 cut(s) 679, 1037
Tsp45I GTSAC 2 cut(s) 937, 1190
TspDTI ATGAA 6 cut(s) 66, 144, 901, 1002, 1076, 1195
TspGWI ACGGA 1 cut(s) 1125
TspRI CASTG 3 cut(s) 259, 805, 1309
XapI RAATTY 2 cut(s) 202, 482
XceI RCATGY 1 cut(s) 933
XmnI GAANNNNTTC 2 cut(s) 96, 532
XspI CTAG 5 cut(s) 218, 333, 372, 561, 576
ZrmI AGTACT 1 cut(s) 712
Zsp2I ATGCAT 2 cut(s) 322, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.