MD14G1147500.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007591
Physical Location & Seq
Reverse (-)
24025342 .. 24030284
4943 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1147500.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGGCGGAAGAGGAGAACGGCTATCCAGATAGCAGGCAAGAGCAAGAAGAAGCCCTCGTCGCTCTGATCGAGCATCGCACCCGGGAAGTCCACCATCTCCGCCAGCGCGTCAACTATTACAAAACTCAGCTTGGAGAAACGGAGAAGAGGTTAACTGATGCCGAAATTAAGTTGGCTCGACTTCGACGCCAAGACAAAGTAGGGTCAACCAGGAGTTCTCCGGGTGGTGGAACGAGAAGTCCAAAGGTGGAGCGCAGATCAACAAGTCCTATACATTTAAATGAAGGTTCTAGACACCAGCATCAATCGAAAACAGAACTTTTCATTCCTGCAGTCAGCTCAAAAGTTTCCCATCCTACAATGGTGGCAGGTTCTAGTGGGAAAAATCCAGTTAATTCTAGTGCTCAAGCAAGTCCATCAGTTTCTTGTCAGTCTATTAGTGCTAACAGAACGAAAGGCGACAAATCGTATAGAAATTCTTCAAAGCAGGACGTTGAAGTTCAAGATAATGGGAAAAAACGGAAATTTGAACAGAAAGAACACAAAGAATTAGTCCCGTTGGTTCGCACTAGCTCTCAACCATGTAAAATCAACTGCTATGCGAGCAGTCACATCTCAAGTCAGCACAAGAGAAAGTTGAGAAGTCTTTCTTTGTGTCCAGTGAATGATCAGCTTTTTGTGACCAGTGCTTTGGATGGAGTAGTCAACTTCTGGCAAGTTCAGTCCAAAGGGTCTAGTGCTTCTCGACTTAGTTCTACTGATTGTGCATCTCCAAACAGGAGATGGCCAGAAGATATCGCCTGGCACCCTGATGGAAACAGACTATTTTCTGTGTATAGTGCTGATGGTGGCGACTCGCAAGTATCAGTCCTAGATTTAAATAGAACACAAGGGAAAGGTCGTGTTACTTTCTTCAAAGATAAGCCTCATGTGAGAGGGATCATTAACAACATAGTTTTCCCACCCTGGGAGGATACCTGTTTTGTCACTGGTGGTAGTGATCATGCTGTTATAATCTGGAAAGAGGCTGATGCGGAGAATGTATGGAAACCAAAGACACTGCATAGGAATATGCACTCATCTGCTGTTATGGGAGTTGCCGGGATGTTCCAAAAGCAGATAATACTATCAGCCGGAGCAGACAAGAGAATTGTTGGGTTTGACGCTAACACAGGAAGAGCAGAGTTTAAGCATCAGATAGAAAGTAAATGCATGAGTGTGTTGCCAAATCCTTGCGACTTCAATATCTTCATGGTTCAATCAGGGACCCATGAGAAGCAGCTCCGACTATTTGATATCAGACTGAGGCAAACAGAGATACATGCTTTTGGGTGGAAGCAAGAGAGCAGTGAATCTCAGTCAGCTCTGATCAATCAGGCTTGGTCTCCTGATGGCTTATACATCTCATCTGGTACGGCAGATCCATTGATCCACATATTCGACATCAGATACAACGCCAACAAGCCTTCTCAATCCATAAGCGCTCATCAGAAACGGGTTTTCAAAGCTGTATGGCTCCACTCATGCCCCCTTCTCATTTCCATATCTTCTGATTTGAACATTGGTTTACACAAAACCACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

528

Amino Acids

58.96

Weight (kDa)

9.43

Isoelectric Point (pI)

63.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 204 - 392 1.1e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 207 - 434 3.1e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 283 - 521 2e-13 Prp19 WD40 domain
EIF3I PF24805 358 - 521 7.8e-06 EIF3I
WD40_MABP1-WDR62_2nd PF24782 359 - 519 6.4e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 364 - 522 1.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 419 - 525 1.5e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 804
AccII CGCG 1 cut(s) 108
AciI CCGC 3 cut(s) 5, 100, 1034
AclWI GGATC 3 cut(s) 947, 1415, 1423
AcoI YGGCCR 1 cut(s) 785
AcsI RAATTY 2 cut(s) 475, 524
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 1 cut(s) 1414
AfeI AGCGCT 1 cut(s) 1483
AfiI CCNNNNNNNGG 2 cut(s) 227, 967
AgsI TTSAA 9 cut(s) 483, 497, 503, 530, 916, 1243, 1259, 1504, 1558
AjnI CCWGG 3 cut(s) 209, 800, 965
AloI GAACNNNNNNTCC 2 cut(s) 223, 255
AluBI AGCT 7 cut(s) 130, 339, 573, 673, 1282, 1364, 1508
AluI AGCT 7 cut(s) 130, 339, 573, 673, 1282, 1364, 1508
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 1389
AlwI GGATC 3 cut(s) 947, 1415, 1423
Ama87I CYCGRG 1 cut(s) 81
Aor51HI AGCGCT 1 cut(s) 1483
AoxI GGCC 1 cut(s) 785
ApeKI GCWGC 1 cut(s) 1279
ApoI RAATTY 2 cut(s) 475, 524
ArsI GACNNNNNNTTYG 4 cut(s) 537, 569, 673, 705
Asp700I GAANNNNTTC 2 cut(s) 478, 646
AspLEI GCGC 3 cut(s) 108, 255, 1484
AspS9I GGNCC 1 cut(s) 1266
AsuC2I CCSGG 4 cut(s) 82, 83, 222, 1102
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 1 cut(s) 1266
BalI TGGCCA 1 cut(s) 787
BanI GGYRCC 1 cut(s) 804
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 1291
BccI CCATC 8 cut(s) 102, 360, 424, 689, 777, 806, 839, 1385
BceAI ACGGC 2 cut(s) 34, 1431
BciT130I CCWGG 3 cut(s) 211, 802, 967
BciVI GTATCC 1 cut(s) 967
BclI TGATCA 3 cut(s) 667, 1000, 1368
BcnI CCSGG 4 cut(s) 82, 83, 222, 1102
BcoDI GTCTC 1 cut(s) 1389
BfaI CTAG 6 cut(s) 291, 375, 399, 570, 735, 872
BfmI CTRYAG 1 cut(s) 330
BfoI RGCGCY 1 cut(s) 1485
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 967
BisI GCNGC 1 cut(s) 1280
BlsI GCNGC 1 cut(s) 1281
Bme1390I CCNGG 7 cut(s) 82, 83, 211, 222, 802, 967, 1102
Bme18I GGWCC 1 cut(s) 1266
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 1266
BmiI GGNNCC 4 cut(s) 806, 1267, 1268, 1517
BmrFI CCNGG 7 cut(s) 82, 83, 211, 222, 802, 967, 1102
BmsI GCATC 6 cut(s) 82, 148, 310, 776, 1021, 1201
BpuEI CTTGAG 2 cut(s) 390, 601
BpuMI CCSGG 4 cut(s) 82, 83, 222, 1102
BsaAI YACGTR 1 cut(s) 1581
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 1389
BsaJI CCNNGG 3 cut(s) 81, 965, 966
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 967
Bse1I ACTGG 4 cut(s) 389, 659, 684, 994
BseBI CCWGG 3 cut(s) 211, 802, 967
BseDI CCNNGG 3 cut(s) 81, 965, 966
BseGI GGATG 3 cut(s) 352, 700, 1110
BseLI CCNNNNNNNGG 2 cut(s) 227, 967
BseMII CTCAG 3 cut(s) 140, 1295, 1370
BseNI ACTGG 4 cut(s) 389, 659, 684, 994
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 1291
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 1 cut(s) 787
BshNI GGYRCC 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 4 cut(s) 82, 221, 1101, 1134
BslFI GGGAC 2 cut(s) 539, 1279
BslI CCNNNNNNNGG 2 cut(s) 227, 967
BsmAI GTCTC 1 cut(s) 1389
BsmFI GGGAC 2 cut(s) 539, 1279
BsnI GGCC 1 cut(s) 787
Bso31I GGTCTC 1 cut(s) 1389
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
BspACI CCGC 3 cut(s) 5, 100, 1034
BspANI GGCC 1 cut(s) 787
BspCNI CTCAG 3 cut(s) 139, 1296, 1369
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 4 cut(s) 806, 1267, 1268, 1517
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 3 cut(s) 947, 1415, 1423
BspQI GCTCTTC 1 cut(s) 1171
BspT107I GGYRCC 1 cut(s) 804
BspTNI GGTCTC 1 cut(s) 1389
BsrI ACTGG 4 cut(s) 389, 659, 684, 994
BssECI CCNNGG 3 cut(s) 81, 965, 966
BssMI GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
BssNI GRCGYC 1 cut(s) 187
Bst2UI CCWGG 3 cut(s) 211, 802, 967
Bst6I CTCTTC 3 cut(s) 3, 140, 1171
BstACI GRCGYC 1 cut(s) 187
BstBAI YACGTR 1 cut(s) 1581
BstC8I GCNNGC 3 cut(s) 35, 104, 604
BstDEI CTNAG 4 cut(s) 126, 749, 1304, 1356
BstF5I GGATG 3 cut(s) 352, 700, 1110
BstFNI CGCG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 1485
BstHHI GCGC 3 cut(s) 108, 255, 1484
BstKTI GATC 8 cut(s) 69, 260, 670, 942, 1003, 1371, 1423, 1431
BstMAI GTCTC 1 cut(s) 1389
BstMBI GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
BstMWI GCNNNNNNNGC 2 cut(s) 59, 603
BstNI CCWGG 3 cut(s) 211, 802, 967
BstNSI RCATGY 1 cut(s) 1325
BstSCI CCNGG 7 cut(s) 80, 81, 209, 220, 800, 965, 1100
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 1291
BstX2I RGATCY 1 cut(s) 1420
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 1 cut(s) 1420
BsuI GTATCC 1 cut(s) 967
BsuRI GGCC 1 cut(s) 787
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 3 cut(s) 352, 700, 1110
BtsI GCAGTG 2 cut(s) 1058, 1354
BtsIMutI CAGTG 5 cut(s) 666, 691, 987, 1058, 1354
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 3 cut(s) 35, 104, 604
CfoI GCGC 3 cut(s) 108, 255, 1484
Cfr13I GGNCC 1 cut(s) 1266
Cfr9I CCCGGG 1 cut(s) 81
CseI GACGC 3 cut(s) 97, 195, 1172
Csp6I GTAC 1 cut(s) 1413
CviAII CATG 8 cut(s) 582, 929, 1004, 1213, 1252, 1271, 1322, 1524
CviQI GTAC 1 cut(s) 1413
DdeI CTNAG 4 cut(s) 126, 749, 1304, 1356
DpnI GATC 8 cut(s) 68, 259, 669, 941, 1002, 1370, 1422, 1430
DpnII GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
DraI TTTAAA 2 cut(s) 279, 879
EaeI YGGCCR 1 cut(s) 785
Eam1104I CTCTTC 3 cut(s) 3, 140, 1171
EarI CTCTTC 3 cut(s) 3, 140, 1171
EciI GGCGGA 2 cut(s) 20, 89
Eco31I GGTCTC 1 cut(s) 1389
Eco32I GATATC 2 cut(s) 796, 1297
Eco47I GGWCC 1 cut(s) 1266
Eco47III AGCGCT 1 cut(s) 1483
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 1266
EcoRII CCWGG 3 cut(s) 209, 800, 965
EcoRV GATATC 2 cut(s) 796, 1297
EcoT22I ATGCAT 1 cut(s) 1214
FaeI CATG 8 cut(s) 585, 932, 1007, 1216, 1255, 1274, 1325, 1527
FalI AAGNNNNNCTT 2 cut(s) 634, 666
FaqI GGGAC 2 cut(s) 539, 1279
FatI CATG 8 cut(s) 581, 928, 1003, 1212, 1251, 1270, 1321, 1523
FbaI TGATCA 3 cut(s) 667, 1000, 1368
Fnu4HI GCNGC 1 cut(s) 1280
FokI GGATG 3 cut(s) 339, 707, 1117
Fsp4HI GCNGC 1 cut(s) 1280
FspBI CTAG 6 cut(s) 291, 375, 399, 570, 735, 872
GlaI GCGC 3 cut(s) 107, 254, 1483
GluI GCNGC 1 cut(s) 1280
HaeII RGCGCY 1 cut(s) 1485
HaeIII GGCC 1 cut(s) 787
HapII CCGG 4 cut(s) 82, 221, 1101, 1134
HgaI GACGC 3 cut(s) 97, 195, 1172
HhaI GCGC 3 cut(s) 108, 255, 1484
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 8 cut(s) 585, 932, 1007, 1216, 1255, 1274, 1325, 1527
Hin6I GCGC 3 cut(s) 106, 253, 1482
HinP1I GCGC 3 cut(s) 106, 253, 1482
HincII GTYRAC 4 cut(s) 112, 153, 207, 706
HindII GTYRAC 4 cut(s) 112, 153, 207, 706
HinfI GANTC 2 cut(s) 854, 1352
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 4 cut(s) 82, 221, 1101, 1134
Hpy166II GTNNAC 6 cut(s) 91, 112, 153, 207, 706, 1568
Hpy188I TCNGA 8 cut(s) 66, 1197, 1286, 1301, 1368, 1448, 1491, 1552
Hpy188III TCNNGA 6 cut(s) 26, 291, 503, 744, 1018, 1388
Hpy8I GTNNAC 6 cut(s) 91, 112, 153, 207, 706, 1568
Hpy99I CGWCG 2 cut(s) 62, 189
HpyAV CCTTC 3 cut(s) 278, 1476, 1541
HpyCH4IV ACGT 2 cut(s) 492, 1580
HpyCH4V TGCA 5 cut(s) 332, 767, 1063, 1075, 1212
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 603
HpyF3I CTNAG 4 cut(s) 126, 749, 1304, 1356
HpySE526I ACGT 2 cut(s) 492, 1580
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 8 cut(s) 585, 932, 1007, 1216, 1255, 1274, 1325, 1527
HspAI GCGC 3 cut(s) 106, 253, 1482
KflI GGGWCCC 1 cut(s) 1266
Ksp22I TGATCA 3 cut(s) 667, 1000, 1368
KspAI GTTAAC 1 cut(s) 153
Kzo9I GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
LguI GCTCTTC 1 cut(s) 1171
LmnI GCTCC 4 cut(s) 250, 1136, 1287, 1521
Lsp1109I GCAGC 1 cut(s) 1291
LweI GCATC 6 cut(s) 82, 148, 310, 776, 1021, 1201
MaeI CTAG 6 cut(s) 291, 375, 399, 570, 735, 872
MaeII ACGT 2 cut(s) 492, 1580
MaeIII GTNAC 4 cut(s) 608, 679, 904, 985
MalI GATC 8 cut(s) 68, 259, 669, 941, 1002, 1370, 1422, 1430
MboI GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
MboII GAAGA 9 cut(s) 20, 59, 157, 471, 803, 904, 1188, 1240, 1539
MflI RGATCY 1 cut(s) 1420
MhlI GDGCHC 1 cut(s) 406
MlsI TGGCCA 1 cut(s) 787
MluCI AATT 6 cut(s) 165, 394, 475, 524, 548, 1149
MluNI TGGCCA 1 cut(s) 787
MlyI GAGTC 1 cut(s) 848
MmeI TCCRAC 1 cut(s) 1309
MnlI CCTC 8 cut(s) 4, 65, 141, 929, 936, 964, 1018, 1299
Mox20I TGGCCA 1 cut(s) 787
Mph1103I ATGCAT 1 cut(s) 1214
MroXI GAANNNNTTC 2 cut(s) 478, 646
MscI TGGCCA 1 cut(s) 787
MseI TTAA 7 cut(s) 152, 168, 278, 393, 878, 945, 1188
MslI CAYNNNNRTG 3 cut(s) 279, 810, 1217
Msp20I TGGCCA 1 cut(s) 787
MspI CCGG 4 cut(s) 82, 221, 1101, 1134
MspR9I CCNGG 7 cut(s) 82, 83, 211, 222, 802, 967, 1102
MvaI CCWGG 3 cut(s) 211, 802, 967
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 59, 603
NciI CCSGG 4 cut(s) 82, 83, 222, 1102
NdeII GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
NlaIII CATG 8 cut(s) 585, 932, 1007, 1216, 1255, 1274, 1325, 1527
NlaIV GGNNCC 4 cut(s) 806, 1267, 1268, 1517
NmuCI GTSAC 3 cut(s) 608, 679, 985
NsiI ATGCAT 1 cut(s) 1214
NspI RCATGY 1 cut(s) 1325
PasI CCCWGGG 1 cut(s) 966
PciSI GCTCTTC 1 cut(s) 1171
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 2 cut(s) 478, 646
PfeI GAWTC 1 cut(s) 1352
PkrI GCNGC 1 cut(s) 1281
PleI GAGTC 1 cut(s) 848
PpsI GAGTC 1 cut(s) 848
Ppu21I YACGTR 1 cut(s) 1581
PpuMI RGGWCCY 1 cut(s) 1266
PsiI TTATAA 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 1266
Psp6I CCWGG 3 cut(s) 209, 800, 965
PspGI CCWGG 3 cut(s) 209, 800, 965
PspN4I GGNNCC 4 cut(s) 806, 1267, 1268, 1517
PspPI GGNCC 1 cut(s) 1266
PspPPI RGGWCCY 1 cut(s) 1266
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 1420
RsaI GTAC 1 cut(s) 1414
RsaNI GTAC 1 cut(s) 1413
RseI CAYNNNNRTG 3 cut(s) 279, 810, 1217
SapI GCTCTTC 1 cut(s) 1171
SaqAI TTAA 7 cut(s) 152, 168, 278, 393, 878, 945, 1188
SatI GCNGC 1 cut(s) 1280
Sau3AI GATC 8 cut(s) 66, 257, 667, 939, 1000, 1368, 1420, 1428
Sau96I GGNCC 1 cut(s) 1266
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 7 cut(s) 82, 83, 211, 222, 802, 967, 1102
SduI GDGCHC 1 cut(s) 406
SfaNI GCATC 6 cut(s) 82, 148, 310, 776, 1021, 1201
SfcI CTRYAG 1 cut(s) 330
SinI GGWCC 1 cut(s) 1266
SmaI CCCGGG 1 cut(s) 83
SmiI ATTTAAAT 2 cut(s) 279, 879
SmiMI CAYNNNNRTG 3 cut(s) 279, 810, 1217
SmlI CTYRAG 2 cut(s) 405, 616
SmoI CTYRAG 2 cut(s) 405, 616
Sse9I AATT 6 cut(s) 165, 394, 475, 524, 548, 1149
SsiI CCGC 3 cut(s) 5, 100, 1034
SspMI CTAG 6 cut(s) 291, 375, 399, 570, 735, 872
StyD4I CCNGG 7 cut(s) 80, 81, 209, 220, 800, 965, 1100
SwaI ATTTAAAT 2 cut(s) 279, 879
TaiI ACGT 2 cut(s) 495, 1583
TaqI TCGA 6 cut(s) 69, 178, 184, 308, 745, 1440
TasI AATT 6 cut(s) 165, 394, 475, 524, 548, 1149
TfiI GAWTC 1 cut(s) 1352
Tru1I TTAA 7 cut(s) 152, 168, 278, 393, 878, 945, 1188
Tru9I TTAA 7 cut(s) 152, 168, 278, 393, 878, 945, 1188
TscAI CASTG 5 cut(s) 666, 691, 994, 1065, 1354
TseFI GTSAC 3 cut(s) 608, 679, 985
TseI GCWGC 1 cut(s) 1279
Tsp45I GTSAC 3 cut(s) 608, 679, 985
TspDTI ATGAA 3 cut(s) 297, 313, 1240
TspGWI ACGGA 2 cut(s) 155, 535
TspMI CCCGGG 1 cut(s) 81
TspRI CASTG 5 cut(s) 666, 691, 994, 1065, 1354
VpaK11BI GGWCC 1 cut(s) 1266
XapI RAATTY 2 cut(s) 475, 524
XbaI TCTAGA 1 cut(s) 290
XceI RCATGY 1 cut(s) 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 780
XmaI CCCGGG 1 cut(s) 81
XmnI GAANNNNTTC 2 cut(s) 478, 646
XspI CTAG 6 cut(s) 291, 375, 399, 570, 735, 872
Zsp2I ATGCAT 1 cut(s) 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.