FvH4_5g09480

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007591
Physical Location & Seq
Forward (+)
5498763 .. 5503304
4542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09480.t1

Sequence Viewer

Length: 1659 bp
ATGAGTGCTCCTCTCAGAAAGCCAACAACAGCAAGTATAGATATTAACGACGACGACGACGACACCATGATGCCGCCGCCGCGGGCGGAAGAGAACGGCAACCCGGATAACAGGCAAGACCAAGAAGAAGCCCTCGTCGCTCTGATTGAACACCGCACTCAGGAAGTCGAACACAATCGCCGCAGACTCCACTACTACCAATCCAAGCTTGAGGAAGCAGAGAAGAGGTTGAATGAGTCTCAAACTAAACTGGCTCGACTTCGATCACAGAAAAAGGCGGTGTCGACTAAGGCTTCTCCGAGTAAGGGATTTAAAACTGTAAAGGAGGAGCGCAGATCAGTGAGTCCTGTCCGGGCTGTCCCTTTAAGAAGCCAGCCTCAATCTAAAACAGAACTTTTGATTCCGGCTGTTGCTCCAAGAGTTTCCCTACCTCCGAATGTGGCAGGGACCAGTGGGAAGGCTCAAGTTAGTTCTGGTTCTCAAGCCAGCGCCTCGGTTTCTAGTCAGTCTAATAGTGCTGGCAAAGAAAAAGGAGATAAGTCTTATAGAAGTTCGTCAAAGCAGGATGTTGAAGTTCAAGATAGAGGGACGAAACGGAAGTTTGAAGAGAAAGAACATAAAGAATTAATTCCGATGGTTCGATCTAGCTCCAGACCCTCTACAATCCAGTGCCATTCAAACAATCATATATCCAGCCATCACAAGAGAAAGTTGAGATGTCTTTATTCGTGCCCAGTGAATGATCATCTTTTTGCAACCAGCGCTTTGGACGGACTGGTCAACTTGTGGGAAGTTCAAGGCAGGGGATCAGGTGCTTCTCTACTTAGTACTACAGATTGCCAATCCCCTAAAAGGAGATGGCCAGAAGATATTGCCTGGCATCCCCATGGAAACAGCCTATTTTCTGTGTATACTGCTGATAGTGGGGAATCACAAGTATCAGTTCTAAATCTAAATAGAACACAAGGGAAACCCCGTGTTACTTTCTTGGAAGCCAAGCCTCATGTGAAAGGAATTATTAACAGCATAGTTTTCCCACCTTGGGGAAATACCTGCTTTGTCACTGGTGGTAGTGATCATGCTGTTGTACTCTGGAAAGAGACAGATGCAGAAAATGTCTGGAAACCAAAGCAATTGCATAGGAGTATGCATTCATCTGCTGTCATGGGAGTTGCTGGGATGTGTCAAAAGCAAATTATACTATCTGTTGGAGCAGACAAGAGGATTGTTGGCTATGATGCTAACATGGAAAGAACAGAGTTCAAGCATCAGATAGATAGTAAATGCATGAGTGTTTTGCCAAATCCTTGCGACTTTAATCTATTCATGGTTCAAGCAGGTACCCACGAGAAGCAGCTCCGCCTTTATGACATCAGACTGAGGCAAACAGAGATACATGCTTTTGGGTGGAAGCAAGAGAGTAGTGATTCTCAATCAGCTCTGATAAATCAAGCTTGGTCTCCTGATGGCTTATACATCTCATCTGGTACTGCTGATCCTATGATTCACATATTTGATATCAGATATAACGCCCAGAAGCCTTCCCAATCCATTAGGGCACATCAGAAACGGGTTTTCAAAGCTGTATGGCTCAGCACTAGCCCCCTTCTCATTTCTATATCTTCTGATCTGACGGTTGGTTTGCACAAGATCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

553

Amino Acids

61.59

Weight (kDa)

9.38

Isoelectric Point (pI)

62.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 228 - 415 8.7e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 233 - 459 1.5e-06 WDR5 beta-propeller domain
WD40_Prp19 PF24814 331 - 509 1.2e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 348 - 529 9e-09 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 384 - 545 4.8e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 389 - 546 2.7e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 443 - 548 7.8e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 485 - 550 2.6e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 485 - 550 1.3e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 776
Acc36I ACCTGC 2 cut(s) 1061, 1328
Acc65I GGTACC 1 cut(s) 1340
AccB1I GGYRCC 1 cut(s) 1340
AccI GTMKAC 2 cut(s) 284, 911
AccII CGCG 1 cut(s) 82
AciI CCGC 9 cut(s) 74, 77, 80, 82, 86, 154, 181, 278, 1360
AclWI GGATC 2 cut(s) 814, 1490
AcoI YGGCCR 1 cut(s) 860
AfaI GTAC 4 cut(s) 829, 1089, 1342, 1489
AfeI AGCGCT 1 cut(s) 763
AfiI CCNNNNNNNGG 5 cut(s) 160, 305, 852, 1042, 1043
AjnI CCWGG 1 cut(s) 875
AluBI AGCT 6 cut(s) 208, 648, 1357, 1439, 1454, 1583
AluI AGCT 6 cut(s) 208, 648, 1357, 1439, 1454, 1583
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 3 cut(s) 243, 1094, 1464
AlwI GGATC 2 cut(s) 814, 1490
Aor51HI AGCGCT 1 cut(s) 763
AoxI GGCC 1 cut(s) 860
ApeKI GCWGC 1 cut(s) 1354
ArsI GACNNNNNNTTYG 2 cut(s) 1624, 1656
AseI ATTAAT 1 cut(s) 626
Asp700I GAANNNNTTC 1 cut(s) 627
Asp718I GGTACC 1 cut(s) 1340
AspLEI GCGC 3 cut(s) 333, 491, 764
AspS9I GGNCC 1 cut(s) 447
AsuC2I CCSGG 2 cut(s) 104, 353
AvaII GGWCC 1 cut(s) 447
BaeGI GKGCMC 2 cut(s) 734, 1561
BalI TGGCCA 1 cut(s) 862
BanI GGYRCC 1 cut(s) 1340
BauI CACGAG 1 cut(s) 1346
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 1366
BccI CCATC 4 cut(s) 628, 705, 852, 1460
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 877
BclI TGATCA 2 cut(s) 742, 1075
BcnI CCSGG 2 cut(s) 104, 353
BcoDI GTCTC 3 cut(s) 243, 1094, 1464
BfaI CTAG 3 cut(s) 501, 645, 1599
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 2 cut(s) 492, 765
BfuAI ACCTGC 2 cut(s) 1061, 1328
BglII AGATCT 1 cut(s) 1650
BisI GCNGC 5 cut(s) 74, 77, 80, 181, 1355
BlpI GCTNAGC 1 cut(s) 1592
BlsI GCNGC 5 cut(s) 75, 78, 81, 182, 1356
BmcAI AGTACT 1 cut(s) 829
Bme1390I CCNGG 3 cut(s) 104, 353, 877
Bme18I GGWCC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 448, 1342
BmrFI CCNGG 3 cut(s) 104, 353, 877
BmrI ACTGGG 1 cut(s) 728
BmsI GCATC 5 cut(s) 60, 889, 1096, 1228, 1276
BmuI ACTGGG 1 cut(s) 728
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 634
Bpu1102I GCTNAGC 1 cut(s) 1592
BpuEI CTTGAG 3 cut(s) 230, 447, 465
BpuMI CCSGG 2 cut(s) 104, 353
BsaI GGTCTC 1 cut(s) 1464
BsaJI CCNNGG 4 cut(s) 80, 492, 886, 1040
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 305, 852, 1042, 1043
Bse1I ACTGG 6 cut(s) 255, 450, 667, 734, 780, 1069
BseBI CCWGG 1 cut(s) 877
BseDI CCNNGG 4 cut(s) 80, 492, 886, 1040
BseGI GGATG 3 cut(s) 571, 880, 1185
BseLI CCNNNNNNNGG 5 cut(s) 160, 305, 852, 1042, 1043
BseMII CTCAG 4 cut(s) 28, 173, 1370, 1606
BseNI ACTGG 6 cut(s) 255, 450, 667, 734, 780, 1069
BseRI GAGGAG 1 cut(s) 341
BseSI GKGCMC 2 cut(s) 734, 1561
BseXI GCAGC 1 cut(s) 1366
BseYI CCCAGC 1 cut(s) 1175
Bsh1236I CGCG 1 cut(s) 82
BshFI GGCC 1 cut(s) 862
BshNI GGYRCC 1 cut(s) 1340
BsiHKAI GWGCWC 1 cut(s) 10
BsiSI CCGG 3 cut(s) 104, 352, 404
BslFI GGGAC 3 cut(s) 344, 460, 601
BslI CCNNNNNNNGG 5 cut(s) 160, 305, 852, 1042, 1043
BsmAI GTCTC 3 cut(s) 243, 1094, 1464
BsmFI GGGAC 3 cut(s) 344, 460, 601
BsmI GAATGC 1 cut(s) 1150
BsnI GGCC 1 cut(s) 862
Bso31I GGTCTC 1 cut(s) 1464
Bsp1286I GDGCHC 3 cut(s) 10, 734, 1561
Bsp143I GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
Bsp1720I GCTNAGC 1 cut(s) 1592
Bsp19I CCATGG 1 cut(s) 886
BspACI CCGC 9 cut(s) 74, 77, 80, 82, 86, 154, 181, 278, 1360
BspANI GGCC 1 cut(s) 862
BspCNI CTCAG 4 cut(s) 27, 172, 1371, 1605
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 448, 1342
BspMI ACCTGC 2 cut(s) 1061, 1328
BspPI GGATC 2 cut(s) 814, 1490
BspT107I GGYRCC 1 cut(s) 1340
BspTNI GGTCTC 1 cut(s) 1464
BsrI ACTGG 6 cut(s) 255, 450, 667, 734, 780, 1069
BssECI CCNNGG 4 cut(s) 80, 492, 886, 1040
BssMI GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
BssNAI GTATAC 1 cut(s) 912
BssSI CACGAG 1 cut(s) 1346
BssT1I CCWWGG 2 cut(s) 886, 1040
Bst1107I GTATAC 1 cut(s) 912
Bst2BI CACGAG 1 cut(s) 1346
Bst2UI CCWGG 1 cut(s) 877
Bst4CI ACNGT 2 cut(s) 319, 1636
Bst6I CTCTTC 3 cut(s) 84, 218, 600
BstC8I GCNNGC 4 cut(s) 84, 374, 487, 520
BstDEI CTNAG 6 cut(s) 14, 159, 288, 824, 1379, 1592
BstDSI CCRYGG 2 cut(s) 80, 886
BstF5I GGATG 3 cut(s) 571, 880, 1185
BstFNI CGCG 1 cut(s) 82
BstH2I RGCGCY 2 cut(s) 492, 765
BstHHI GCGC 3 cut(s) 333, 491, 764
BstKTI GATC 9 cut(s) 266, 338, 644, 745, 809, 1078, 1498, 1630, 1653
BstMAI GTCTC 3 cut(s) 243, 1094, 1464
BstMBI GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
BstMWI GCNNNNNNNGC 3 cut(s) 79, 137, 761
BstNI CCWGG 1 cut(s) 877
BstNSI RCATGY 1 cut(s) 1400
BstSCI CCNGG 3 cut(s) 102, 351, 875
BstSFI CTRYAG 1 cut(s) 831
BstSLI GKGCMC 2 cut(s) 734, 1561
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 1366
BstX2I RGATCY 1 cut(s) 1650
BstXI CCANNNNNNTGG 1 cut(s) 766
BstYI RGATCY 1 cut(s) 1650
BstZ17I GTATAC 1 cut(s) 912
BsuRI GGCC 1 cut(s) 862
BtgI CCRYGG 2 cut(s) 80, 886
BtsCI GGATG 3 cut(s) 571, 880, 1185
BtsIMutI CAGTG 5 cut(s) 345, 457, 674, 741, 1062
BveI ACCTGC 2 cut(s) 1061, 1328
Cac8I GCNNGC 4 cut(s) 84, 374, 487, 520
CfoI GCGC 3 cut(s) 333, 491, 764
Cfr13I GGNCC 1 cut(s) 447
Cfr42I CCGCGG 1 cut(s) 83
Csp6I GTAC 4 cut(s) 828, 1088, 1341, 1488
CviAII CATG 9 cut(s) 67, 887, 1004, 1079, 1165, 1246, 1288, 1327, 1397
CviQI GTAC 4 cut(s) 828, 1088, 1341, 1488
DdeI CTNAG 6 cut(s) 14, 159, 288, 824, 1379, 1592
DpnI GATC 9 cut(s) 265, 337, 643, 744, 808, 1077, 1497, 1629, 1652
DpnII GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
DraI TTTAAA 1 cut(s) 313
DrdI GACNNNNNNGTC 1 cut(s) 776
DseDI GACNNNNNNGTC 1 cut(s) 776
EaeI YGGCCR 1 cut(s) 860
Eam1104I CTCTTC 3 cut(s) 84, 218, 600
EarI CTCTTC 3 cut(s) 84, 218, 600
EciI GGCGGA 2 cut(s) 101, 1349
Eco130I CCWWGG 2 cut(s) 886, 1040
Eco31I GGTCTC 1 cut(s) 1464
Eco32I GATATC 1 cut(s) 1519
Eco47I GGWCC 1 cut(s) 447
Eco47III AGCGCT 1 cut(s) 763
EcoRII CCWGG 1 cut(s) 875
EcoRV GATATC 1 cut(s) 1519
EcoT14I CCWWGG 2 cut(s) 886, 1040
EcoT22I ATGCAT 2 cut(s) 1152, 1289
ErhI CCWWGG 2 cut(s) 886, 1040
FaeI CATG 9 cut(s) 70, 890, 1007, 1082, 1168, 1249, 1291, 1330, 1400
FaqI GGGAC 3 cut(s) 344, 460, 601
FatI CATG 9 cut(s) 66, 886, 1003, 1078, 1164, 1245, 1287, 1326, 1396
FauI CCCGC 1 cut(s) 75
FbaI TGATCA 2 cut(s) 742, 1075
FblI GTMKAC 2 cut(s) 284, 911
Fnu4HI GCNGC 5 cut(s) 74, 77, 80, 181, 1355
FokI GGATG 3 cut(s) 578, 867, 1192
Fsp4HI GCNGC 5 cut(s) 74, 77, 80, 181, 1355
FspBI CTAG 3 cut(s) 501, 645, 1599
GlaI GCGC 3 cut(s) 332, 490, 763
GluI GCNGC 5 cut(s) 74, 77, 80, 181, 1355
GsaI CCCAGC 1 cut(s) 1179
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 2 cut(s) 492, 765
HaeIII GGCC 1 cut(s) 862
HapII CCGG 3 cut(s) 104, 352, 404
HhaI GCGC 3 cut(s) 333, 491, 764
Hin1II CATG 9 cut(s) 70, 890, 1007, 1082, 1168, 1249, 1291, 1330, 1400
Hin6I GCGC 3 cut(s) 331, 489, 762
HinP1I GCGC 3 cut(s) 331, 489, 762
HincII GTYRAC 2 cut(s) 285, 781
HindII GTYRAC 2 cut(s) 285, 781
HindIII AAGCTT 2 cut(s) 206, 1452
HinfI GANTC 7 cut(s) 186, 236, 343, 400, 929, 1427, 1504
HpaII CCGG 3 cut(s) 104, 352, 404
Hpy166II GTNNAC 3 cut(s) 285, 781, 912
Hpy188III TCNNGA 6 cut(s) 161, 578, 651, 1093, 1120, 1463
Hpy8I GTNNAC 3 cut(s) 285, 781, 912
Hpy99I CGWCG 5 cut(s) 53, 56, 59, 62, 140
HpyAV CCTTC 3 cut(s) 451, 1551, 1616
HpyCH4III ACNGT 2 cut(s) 319, 1636
HpyCH4V TGCA 6 cut(s) 755, 1109, 1138, 1150, 1287, 1645
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 137, 761
HpyF3I CTNAG 6 cut(s) 14, 159, 288, 824, 1379, 1592
Hsp92II CATG 9 cut(s) 70, 890, 1007, 1082, 1168, 1249, 1291, 1330, 1400
HspAI GCGC 3 cut(s) 331, 489, 762
KpnI GGTACC 1 cut(s) 1344
Ksp22I TGATCA 2 cut(s) 742, 1075
KspI CCGCGG 1 cut(s) 83
Kzo9I GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
LmnI GCTCC 6 cut(s) 13, 328, 418, 653, 1211, 1362
Lsp1109I GCAGC 1 cut(s) 1366
LweI GCATC 5 cut(s) 60, 889, 1096, 1228, 1276
MaeI CTAG 3 cut(s) 501, 645, 1599
MaeIII GTNAC 2 cut(s) 979, 1060
MalI GATC 9 cut(s) 265, 337, 643, 744, 808, 1077, 1497, 1629, 1652
MboI GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
MboII GAAGA 6 cut(s) 101, 137, 235, 617, 878, 1614
MfeI CAATTG 1 cut(s) 1133
MflI RGATCY 1 cut(s) 1650
MhlI GDGCHC 3 cut(s) 10, 734, 1561
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 5 cut(s) 623, 627, 1014, 1133, 1194
MluNI TGGCCA 1 cut(s) 862
MlyI GAGTC 3 cut(s) 180, 245, 352
MmeI TCCRAC 1 cut(s) 1189
Mox20I TGGCCA 1 cut(s) 862
Mph1103I ATGCAT 2 cut(s) 1152, 1289
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 1 cut(s) 862
MseI TTAA 6 cut(s) 45, 312, 365, 626, 1020, 1317
MslI CAYNNNNRTG 2 cut(s) 68, 885
Msp20I TGGCCA 1 cut(s) 862
MspA1I CMGCKG 1 cut(s) 82
MspI CCGG 3 cut(s) 104, 352, 404
MspR9I CCNGG 3 cut(s) 104, 353, 877
MunI CAATTG 1 cut(s) 1133
Mva1269I GAATGC 1 cut(s) 1150
MvaI CCWGG 1 cut(s) 877
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 79, 137, 761
NciI CCSGG 2 cut(s) 104, 353
NcoI CCATGG 1 cut(s) 886
NdeII GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
NlaIII CATG 9 cut(s) 70, 890, 1007, 1082, 1168, 1249, 1291, 1330, 1400
NlaIV GGNNCC 2 cut(s) 448, 1342
NmuCI GTSAC 1 cut(s) 1060
NsiI ATGCAT 2 cut(s) 1152, 1289
NspI RCATGY 1 cut(s) 1400
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 57
PctI GAATGC 1 cut(s) 1150
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 4 cut(s) 400, 929, 1427, 1504
PkrI GCNGC 5 cut(s) 75, 78, 81, 182, 1356
PleI GAGTC 3 cut(s) 180, 244, 351
PpsI GAGTC 3 cut(s) 180, 244, 351
PshBI ATTAAT 1 cut(s) 626
Psp6I CCWGG 1 cut(s) 875
PspFI CCCAGC 1 cut(s) 1175
PspGI CCWGG 1 cut(s) 875
PspN4I GGNNCC 2 cut(s) 448, 1342
PspPI GGNCC 1 cut(s) 447
PsuI RGATCY 1 cut(s) 1650
RsaI GTAC 4 cut(s) 829, 1089, 1342, 1489
RsaNI GTAC 4 cut(s) 828, 1088, 1341, 1488
RseI CAYNNNNRTG 2 cut(s) 68, 885
SacII CCGCGG 1 cut(s) 83
SalI GTCGAC 1 cut(s) 283
SaqAI TTAA 6 cut(s) 45, 312, 365, 626, 1020, 1317
SatI GCNGC 5 cut(s) 74, 77, 80, 181, 1355
Sau3AI GATC 9 cut(s) 263, 335, 641, 742, 806, 1075, 1495, 1627, 1650
Sau96I GGNCC 1 cut(s) 447
ScaI AGTACT 1 cut(s) 829
SchI GAGTC 3 cut(s) 180, 245, 352
ScrFI CCNGG 3 cut(s) 104, 353, 877
SduI GDGCHC 3 cut(s) 10, 734, 1561
SfaNI GCATC 5 cut(s) 60, 889, 1096, 1228, 1276
SfcI CTRYAG 1 cut(s) 831
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 68, 885
SmlI CTYRAG 3 cut(s) 209, 462, 480
SmoI CTYRAG 3 cut(s) 209, 462, 480
Sse9I AATT 5 cut(s) 623, 627, 1014, 1133, 1194
SsiI CCGC 9 cut(s) 74, 77, 80, 82, 86, 154, 181, 278, 1360
SspMI CTAG 3 cut(s) 501, 645, 1599
StyD4I CCNGG 3 cut(s) 102, 351, 875
StyI CCWWGG 2 cut(s) 886, 1040
TaaI ACNGT 2 cut(s) 319, 1636
TaqI TCGA 5 cut(s) 168, 256, 262, 284, 640
TasI AATT 5 cut(s) 623, 627, 1014, 1133, 1194
TatI WGTACW 2 cut(s) 827, 1087
TauI GCSGC 4 cut(s) 76, 79, 82, 183
TfiI GAWTC 4 cut(s) 400, 929, 1427, 1504
Tru1I TTAA 6 cut(s) 45, 312, 365, 626, 1020, 1317
Tru9I TTAA 6 cut(s) 45, 312, 365, 626, 1020, 1317
TscAI CASTG 5 cut(s) 345, 457, 674, 741, 1069
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 1 cut(s) 1354
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 2 cut(s) 1143, 1315
TspGWI ACGGA 2 cut(s) 610, 786
TspRI CASTG 5 cut(s) 345, 457, 674, 741, 1069
VpaK11BI GGWCC 1 cut(s) 447
VspI ATTAAT 1 cut(s) 626
XceI RCATGY 1 cut(s) 1400
XmiI GTMKAC 2 cut(s) 284, 911
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 3 cut(s) 501, 645, 1599
ZrmI AGTACT 1 cut(s) 829
Zsp2I ATGCAT 2 cut(s) 1152, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.