Rh7AG097900

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007591
Physical Location & Seq
Reverse (-)
7444353 .. 7449307
4955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG097900.1

Sequence Viewer

Length: 1326 bp
ATGAGTGCTCTTCTCAAAAACCCCAGAAGAGAAAGCGTAGAGATTGACGACGACGACACCATGATGCCGCCGGCGGAAGAGAACGGCGACCCAGATAGCAGACAAGACCAAGAAGACGCCCTCATCGCTCTGATTGAGCACCGCGCTCAGGAAGTGGAACACAATCGCCGGAGACGGGAGTATTACCAATCCAAGCTTGTTGAAGCAGAGAAGAGGTTGAATGAATCTCAAACTAAATTGGCTCGACTTCGATCCCAAAACAAGGCAGCGTCGACTAAGGTGTCTCCGAGTAATGGAATTAAAAGTGTAAAGGAGGAGCGCAGATCAATGAGTCCTATCCCTTTAAGAAGCCGGCCTCAATCTAAAACAGAACTTTTAATTCCTGCTGTCACTCCAAAAGTTTCCCAACCTCCGAAAGTGGCAGGGTCTAGTGGGAAGGCACAAGTTAGTTCTAGTTCTCAAGCCAGCCCGGCAGTTTCTAGTCAGTCTATTAGTGCTAGCAAAGAAAAAGGAGATAAGTCTTATAGAAGTTCGTCAAAGCAGGATGTTGAAGTTCAAGATAAAGGGACAAAACGGAAGTTCGAGGAGAAAGAACATAAAGAATTAATTCCGATGGTTCGTACTAGCTCCAAACCCTCGATAATCGAGTGCCATTCAAACAATCACATATCCAGTCATCACAAGAGAAAGTTGAGATGTCTTTATATGTGTCCAGTGAATGATCAGCTTTTTGCAACCAGCGCTTTGGATGGACTGGTCAACTTGTGGGAAGTTCAAGGCAGGGGGTCAGGTGCTTCTCTACTTAGTACTACAGATTGCCAATCCCCAAAGCAAAGGAGATGGCCAGAAGATATTGCCTGGCATCCCCATGGAAACAGCCTATTTTCTGTGTATACTGCTGATGGTGGGGAATCACAAGTATCAGTTCTAAATCTAAATCGAACACAAGGGAAACCTCGTGTTACTTTCTTGGAAGCCAAGCCTCATGTGAAAGGGATTATTAACAGCATAGTTTTCCCACCTTGGGAGAATACCTGCTTTGTCACTGGTGGTAGTGATCATGCTGTTGTACTCTGGAAAGAGACGGATGCAGAAAATGTCTGGAAACCAAAGCAATTGCATAGGAGTATACATTCATCTGCTGTTATGGGCGTTGCTGGGATGTGGCAAAAGCAAATTATACTATCTGTGGGAGCAGACAAGAGGATTGTTGGCTTCAATGCTAATATGGAAAGAACAGAGTTTAAGCATCAGATAGATAGTAAATGCATGAGTGTTTTGCCAAATCCTTGCGACTTTAATCTATTCATGGTTCAAGCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

441

Amino Acids

49.24

Weight (kDa)

9.21

Isoelectric Point (pI)

66.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 222 - 403 9.4e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
Acc36I ACCTGC 1 cut(s) 1043
AccI GTMKAC 3 cut(s) 272, 893, 1129
AccII CGCG 1 cut(s) 144
AciI CCGC 3 cut(s) 68, 74, 142
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 1 cut(s) 842
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 3 cut(s) 622, 808, 1071
AfeI AGCGCT 1 cut(s) 742
AfiI CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
AgsI TTSAA 8 cut(s) 203, 220, 551, 557, 657, 776, 1219, 1316
AjnI CCWGG 1 cut(s) 857
AluBI AGCT 3 cut(s) 196, 627, 727
AluI AGCT 3 cut(s) 196, 627, 727
Alw21I GWGCWC 2 cut(s) 10, 141
Alw26I GTCTC 3 cut(s) 166, 288, 1076
AlwI GGATC 1 cut(s) 246
Aor51HI AGCGCT 1 cut(s) 742
AoxI GGCC 2 cut(s) 353, 842
ApeKI GCWGC 1 cut(s) 266
AseI ATTAAT 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 3 cut(s) 146, 321, 743
AsuC2I CCSGG 1 cut(s) 470
AsuNHI GCTAGC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 844
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 10, 141
BbvI GCAGC 1 cut(s) 278
BccI CCATC 4 cut(s) 607, 743, 834, 896
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 859
BclI TGATCA 2 cut(s) 721, 1057
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 3 cut(s) 166, 288, 1076
BfaI CTAG 5 cut(s) 429, 453, 480, 498, 624
BfmI CTRYAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 744
BfuAI ACCTGC 1 cut(s) 1043
BglI GCCNNNNNGGC 1 cut(s) 470
BisI GCNGC 2 cut(s) 68, 267
BlsI GCNGC 2 cut(s) 69, 268
BmcAI AGTACT 1 cut(s) 808
Bme1390I CCNGG 2 cut(s) 470, 859
BmrFI CCNGG 2 cut(s) 470, 859
BmsI GCATC 4 cut(s) 54, 871, 1078, 1258
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 868, 1022
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
Bse118I RCCGGY 2 cut(s) 70, 351
Bse1I ACTGG 4 cut(s) 672, 713, 759, 1051
BseBI CCWGG 1 cut(s) 859
BseDI CCNNGG 2 cut(s) 868, 1022
BseGI GGATG 5 cut(s) 550, 754, 862, 1093, 1167
BseLI CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 4 cut(s) 672, 713, 759, 1051
BseRI GAGGAG 2 cut(s) 329, 599
BseXI GCAGC 1 cut(s) 278
BseYI CCCAGC 1 cut(s) 1157
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 355, 844
BsiHKAI GWGCWC 2 cut(s) 10, 141
BsiSI CCGG 4 cut(s) 71, 169, 352, 470
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
BsmAI GTCTC 3 cut(s) 166, 288, 1076
BsmBI CGTCTC 2 cut(s) 166, 1076
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 2 cut(s) 355, 844
Bsp1286I GDGCHC 2 cut(s) 10, 141
Bsp143I GATC 4 cut(s) 251, 323, 721, 1057
Bsp19I CCATGG 1 cut(s) 868
BspACI CCGC 3 cut(s) 68, 74, 142
BspANI GGCC 2 cut(s) 355, 844
BspCNI CTCAG 1 cut(s) 160
BspFNI CGCG 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 1043
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 1 cut(s) 246
BspQI GCTCTTC 1 cut(s) 15
BsrFI RCCGGY 2 cut(s) 70, 351
BsrI ACTGG 4 cut(s) 672, 713, 759, 1051
BssAI RCCGGY 2 cut(s) 70, 351
BssECI CCNNGG 2 cut(s) 868, 1022
BssMI GATC 4 cut(s) 251, 323, 721, 1057
BssNAI GTATAC 2 cut(s) 894, 1130
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 2 cut(s) 868, 1022
Bst1107I GTATAC 2 cut(s) 894, 1130
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 859
Bst6I CTCTTC 4 cut(s) 15, 22, 72, 206
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 4 cut(s) 72, 353, 466, 499
BstDEI CTNAG 3 cut(s) 147, 276, 803
BstDSI CCRYGG 1 cut(s) 868
BstF5I GGATG 5 cut(s) 550, 754, 862, 1093, 1167
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 744
BstHHI GCGC 3 cut(s) 146, 321, 743
BstKTI GATC 4 cut(s) 254, 326, 724, 1060
BstMAI GTCTC 3 cut(s) 166, 288, 1076
BstMBI GATC 4 cut(s) 251, 323, 721, 1057
BstMWI GCNNNNNNNGC 3 cut(s) 125, 470, 740
BstNI CCWGG 1 cut(s) 859
BstSCI CCNGG 2 cut(s) 468, 857
BstSFI CTRYAG 1 cut(s) 810
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 745
BstZ17I GTATAC 2 cut(s) 894, 1130
BsuRI GGCC 2 cut(s) 355, 844
BtgI CCRYGG 1 cut(s) 868
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 5 cut(s) 550, 754, 862, 1093, 1167
BtsIMutI CAGTG 2 cut(s) 720, 1044
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 4 cut(s) 72, 353, 466, 499
CfoI GCGC 3 cut(s) 146, 321, 743
Cfr10I RCCGGY 2 cut(s) 70, 351
CseI GACGC 2 cut(s) 125, 258
Csp6I GTAC 3 cut(s) 621, 807, 1070
CviAII CATG 6 cut(s) 61, 869, 986, 1061, 1270, 1309
CviQI GTAC 3 cut(s) 621, 807, 1070
DdeI CTNAG 3 cut(s) 147, 276, 803
DpnI GATC 4 cut(s) 253, 325, 723, 1059
DpnII GATC 4 cut(s) 251, 323, 721, 1057
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 842
Eam1104I CTCTTC 4 cut(s) 15, 22, 72, 206
EarI CTCTTC 4 cut(s) 15, 22, 72, 206
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 868, 1022
Eco47III AGCGCT 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 857
EcoT14I CCWWGG 2 cut(s) 868, 1022
EcoT22I ATGCAT 1 cut(s) 1271
ErhI CCWWGG 2 cut(s) 868, 1022
Esp3I CGTCTC 2 cut(s) 166, 1076
FaeI CATG 6 cut(s) 64, 872, 989, 1064, 1273, 1312
FaqI GGGAC 1 cut(s) 580
FatI CATG 6 cut(s) 60, 868, 985, 1060, 1269, 1308
FbaI TGATCA 2 cut(s) 721, 1057
FblI GTMKAC 3 cut(s) 272, 893, 1129
Fnu4HI GCNGC 2 cut(s) 68, 267
FokI GGATG 5 cut(s) 557, 761, 849, 1100, 1174
Fsp4HI GCNGC 2 cut(s) 68, 267
FspBI CTAG 5 cut(s) 429, 453, 480, 498, 624
GlaI GCGC 3 cut(s) 145, 320, 742
GluI GCNGC 2 cut(s) 68, 267
GsaI CCCAGC 1 cut(s) 1161
HaeII RGCGCY 1 cut(s) 744
HaeIII GGCC 2 cut(s) 355, 844
HapII CCGG 4 cut(s) 71, 169, 352, 470
HgaI GACGC 2 cut(s) 125, 258
HhaI GCGC 3 cut(s) 146, 321, 743
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 6 cut(s) 64, 872, 989, 1064, 1273, 1312
Hin6I GCGC 3 cut(s) 144, 319, 741
HinP1I GCGC 3 cut(s) 144, 319, 741
HincII GTYRAC 2 cut(s) 273, 760
HindII GTYRAC 2 cut(s) 273, 760
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 3 cut(s) 224, 331, 911
HpaII CCGG 4 cut(s) 71, 169, 352, 470
Hpy166II GTNNAC 4 cut(s) 273, 760, 894, 1130
Hpy188I TCNGA 5 cut(s) 132, 288, 414, 612, 1254
Hpy188III TCNNGA 4 cut(s) 149, 557, 1075, 1102
Hpy8I GTNNAC 4 cut(s) 273, 760, 894, 1130
Hpy99I CGWCG 3 cut(s) 53, 56, 274
HpyAV CCTTC 1 cut(s) 430
HpyCH4V TGCA 4 cut(s) 734, 1091, 1120, 1269
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 470, 740
HpyF3I CTNAG 3 cut(s) 147, 276, 803
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 6 cut(s) 64, 872, 989, 1064, 1273, 1312
HspAI GCGC 3 cut(s) 144, 319, 741
KroI GCCGGC 2 cut(s) 70, 351
KroNI GCCGGC 2 cut(s) 72, 353
Ksp22I TGATCA 2 cut(s) 721, 1057
Kzo9I GATC 4 cut(s) 251, 323, 721, 1057
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 316, 632, 1193
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 4 cut(s) 54, 871, 1078, 1258
MaeI CTAG 5 cut(s) 429, 453, 480, 498, 624
MaeIII GTNAC 3 cut(s) 388, 961, 1042
MalI GATC 4 cut(s) 253, 325, 723, 1059
MboI GATC 4 cut(s) 251, 323, 721, 1057
MboII GAAGA 5 cut(s) 39, 89, 125, 223, 860
MfeI CAATTG 1 cut(s) 1115
MhlI GDGCHC 2 cut(s) 10, 141
MlsI TGGCCA 1 cut(s) 844
MluCI AATT 7 cut(s) 236, 297, 378, 602, 606, 1115, 1176
MluNI TGGCCA 1 cut(s) 844
MlyI GAGTC 1 cut(s) 340
Mox20I TGGCCA 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 1271
MreI CGCCGGCG 1 cut(s) 70
MroNI GCCGGC 2 cut(s) 70, 351
MroXI GAANNNNTTC 1 cut(s) 606
MscI TGGCCA 1 cut(s) 844
MseI TTAA 7 cut(s) 300, 344, 377, 605, 1002, 1245, 1299
MslI CAYNNNNRTG 2 cut(s) 62, 867
Msp20I TGGCCA 1 cut(s) 844
MspI CCGG 4 cut(s) 71, 169, 352, 470
MspR9I CCNGG 2 cut(s) 470, 859
MunI CAATTG 1 cut(s) 1115
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 125, 470, 740
NaeI GCCGGC 2 cut(s) 72, 353
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 868
NdeII GATC 4 cut(s) 251, 323, 721, 1057
NgoMIV GCCGGC 2 cut(s) 70, 351
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 6 cut(s) 64, 872, 989, 1064, 1273, 1312
NmuCI GTSAC 2 cut(s) 388, 1042
NsiI ATGCAT 1 cut(s) 1271
PciSI GCTCTTC 1 cut(s) 15
PdiI GCCGGC 2 cut(s) 72, 353
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 224, 911
PkrI GCNGC 2 cut(s) 69, 268
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PshBI ATTAAT 1 cut(s) 605
Psp6I CCWGG 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 1 cut(s) 857
RsaI GTAC 3 cut(s) 622, 808, 1071
RsaNI GTAC 3 cut(s) 621, 807, 1070
RseI CAYNNNNRTG 2 cut(s) 62, 867
SalI GTCGAC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 7 cut(s) 300, 344, 377, 605, 1002, 1245, 1299
SatI GCNGC 2 cut(s) 68, 267
Sau3AI GATC 4 cut(s) 251, 323, 721, 1057
ScaI AGTACT 1 cut(s) 808
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 470, 859
SduI GDGCHC 2 cut(s) 10, 141
SfaNI GCATC 4 cut(s) 54, 871, 1078, 1258
SfcI CTRYAG 1 cut(s) 810
SgrAI CRCCGGYG 1 cut(s) 70
SmiMI CAYNNNNRTG 2 cut(s) 62, 867
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 7 cut(s) 236, 297, 378, 602, 606, 1115, 1176
SsiI CCGC 3 cut(s) 68, 74, 142
SspMI CTAG 5 cut(s) 429, 453, 480, 498, 624
StyD4I CCNGG 2 cut(s) 468, 857
StyI CCWWGG 2 cut(s) 868, 1022
TaqI TCGA 7 cut(s) 244, 250, 272, 582, 638, 645, 940
TasI AATT 7 cut(s) 236, 297, 378, 602, 606, 1115, 1176
TatI WGTACW 2 cut(s) 806, 1069
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 2 cut(s) 224, 911
Tru1I TTAA 7 cut(s) 300, 344, 377, 605, 1002, 1245, 1299
Tru9I TTAA 7 cut(s) 300, 344, 377, 605, 1002, 1245, 1299
TscAI CASTG 2 cut(s) 720, 1051
TseFI GTSAC 2 cut(s) 388, 1042
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 2 cut(s) 388, 1042
TspDTI ATGAA 3 cut(s) 237, 1125, 1297
TspGWI ACGGA 2 cut(s) 589, 1100
TspRI CASTG 2 cut(s) 720, 1051
VspI ATTAAT 1 cut(s) 605
XmiI GTMKAC 3 cut(s) 272, 893, 1129
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 5 cut(s) 429, 453, 480, 498, 624
ZrmI AGTACT 1 cut(s) 808
Zsp2I ATGCAT 1 cut(s) 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.