MD06G1130900.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007591
Physical Location & Seq
Reverse (-)
27451201 .. 27455714
4514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1130900.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGAGTGCTCTGAAGAAGCCCAAGACGGAGGACGTAGAAATCATAGACGGCGGCGGCGAACAAGCAGCAAACAAGGTGAAGATGGCGGAGGAGGAGAACGGCTACCCAGATAGCAGACAGGAGCAAGAAGAAGCCCTTGTTGCTCTGATCGAGCATCGCACCCGCGAAGTCCACCACCTTCGCAACCGCGTCTCCTATTACAAAACTCAGCTTGGGGAAGCAGAGAGGAGGTTAACTGATACTGAAATTAAGTTGGCTCGACTTCGACGCCAAGACAGAGTAGGGTCAACTAGGAGTTCACCCGGTACTGGAACGAATAGTGTAAAGGTGGAGTGCAGATCAACAAGTCCTATCCATTTATATGAAGGTTCTTCTAGACACCAGCATCAATCCAAAACAGAACTTTTCATTCCTGCTGTCGGCCCAAAAGTTTCCCATCCTACAACGGTGGCAGGGTCTAGTGGGAAAGCTCCAGTTAGTTCTAGTGCTCAAGCAAGTCCATCGGTTTCTAGTCAGTCTGTTAGTGCTAACAGAATGAAAGGGGACAAATCTTATAGAAATTCTTCAAAGCAGGAAGTTGAAGTTCAAGATAAAGGGACAAAACGGAAATTTGAAAAGAAAGAACACAAAGAATTAGTGCCATTGGTTCACACTAGCTCTCAACCATGTAAAATCAACTGCTATGCAAGCAGTCACATCTCAAGTCAGCACAAGAGAAAGTTGAGAAGTCTTTCTTTGTGCCCAGTGAATGATCAGCTTTTTGTGACCAGTGCTTTGGATGGAGTAGTCAACTTCTGGCAAGTTCAGTCCAAGGGGTCTAGTGCTTCTCGGCTTAGTTCTACTGATTGTGTATCCCCAAACAGGAGATGGCCAGAAGATATTGCCTGGCATCCTGATGGAAACAGACTATTTTCTGTGTATAGTGCTGATGGTGGTGACTCGCAAGTATCGGTTCTAGATCTAAATATAACACAAGGGAAAGGTCGTGTTACTTTCTTTGAAGATAAGCCTCATGTGAGAGGGATCATTAACAACATAGTTTTTCCCCCTTGGGAGGATACCTGTTTTGTCACTGGTGGTAGTGATCATGCTGTTATAATCTGGAAAGAGACGGATGCGGAGAATGTATGGAAACCAAAGACATTGCATAGGAATATGCATTCATCTGCTGTTATGGGAGTAGCCGGGATGTTCCAAAAGCAGATGATACTATCAGCTGGAGCAGACAAGAGAATTGTTGGGTTTGACGCTAACACGGGAAGAGCAGAGTTTAAGCATCAGATAGAAAGTAAATGCATGAGTGTCTTGCCAAATCCTTGCGACTTTAATCTCTTCATGGTTCAATCAGGGACGCATGAGAAGCAGCTCCGACTATTTGATATCAGACTGAGGCAAACAGAAATCCATGCTTTTGGGTGGAAGCAAGAGAGCAGTGAATCTCAGTCAGCTCTGATCAATCAGGCCTGGTCTCCCGACGGCTTATACATCTCATCTGGTACCGCAGATCCATTGATCCACATATTCGATATCAGATACAATGCCAACAAGCCTTCTCAGTCTATAAGTGCTCATCAGAAACGGGTTTTCAAAGCTGTATGGCTCCACTCATGCCCCCTTCTCATTTCCATATCTTCTGATTTGAACATTGGTCTACACAAAACCACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

556

Amino Acids

61.79

Weight (kDa)

9.14

Isoelectric Point (pI)

62.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 232 - 420 4.4e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 235 - 462 4.1e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 311 - 549 5.2e-13 Prp19 WD40 domain
EIF3I PF24805 385 - 549 4.6e-06 EIF3I
WD40_MABP1-WDR62_2nd PF24782 387 - 547 4.6e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 392 - 550 1.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 441 - 528 7e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 447 - 553 1.8e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1097
Acc65I GGTACC 1 cut(s) 1496
AccB1I GGYRCC 1 cut(s) 1496
AccI GTMKAC 1 cut(s) 1651
AccII CGCG 2 cut(s) 165, 189
AciI CCGC 7 cut(s) 51, 54, 86, 163, 187, 1118, 1500
AclWI GGATC 3 cut(s) 1031, 1499, 1507
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 2 cut(s) 559, 608
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 268
AfaI GTAC 2 cut(s) 307, 1498
AfiI CCNNNNNNNGG 4 cut(s) 308, 419, 861, 1054
AgsI TTSAA 8 cut(s) 567, 581, 587, 614, 1001, 1343, 1588, 1642
AjnI CCWGG 2 cut(s) 884, 1463
AluBI AGCT 8 cut(s) 211, 470, 657, 757, 1217, 1366, 1448, 1592
AluI AGCT 8 cut(s) 211, 470, 657, 757, 1217, 1366, 1448, 1592
Alw21I GWGCWC 3 cut(s) 10, 490, 1570
Alw26I GTCTC 3 cut(s) 196, 1103, 1473
AlwI GGATC 3 cut(s) 1031, 1499, 1507
AoxI GGCC 3 cut(s) 421, 869, 1461
ApeKI GCWGC 2 cut(s) 65, 1363
ApoI RAATTY 2 cut(s) 559, 608
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
Asp700I GAANNNNTTC 2 cut(s) 562, 730
Asp718I GGTACC 1 cut(s) 1496
AspS9I GGNCC 1 cut(s) 422
AsuC2I CCSGG 2 cut(s) 303, 1186
AsuHPI GGTGA 3 cut(s) 88, 291, 947
BaeGI GKGCMC 1 cut(s) 743
BalI TGGCCA 1 cut(s) 871
BanI GGYRCC 1 cut(s) 1496
Bbv12I GWGCWC 3 cut(s) 10, 490, 1570
BbvI GCAGC 2 cut(s) 77, 1375
BccI CCATC 7 cut(s) 76, 444, 508, 773, 861, 890, 923
BceAI ACGGC 3 cut(s) 64, 115, 1492
BcgI CGANNNNNNTGC 2 cut(s) 483, 517
BciT130I CCWGG 2 cut(s) 886, 1465
BciVI GTATCC 2 cut(s) 862, 1051
BclI TGATCA 3 cut(s) 751, 1084, 1452
BcnI CCSGG 2 cut(s) 303, 1186
BcoDI GTCTC 3 cut(s) 196, 1103, 1473
BfaI CTAG 8 cut(s) 291, 375, 459, 483, 510, 654, 819, 956
BfuI GTATCC 2 cut(s) 862, 1051
BglII AGATCT 1 cut(s) 958
BisI GCNGC 4 cut(s) 52, 55, 66, 1364
BlsI GCNGC 4 cut(s) 53, 56, 67, 1365
Bme1390I CCNGG 4 cut(s) 303, 886, 1186, 1465
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 1498, 1601
BmrFI CCNGG 4 cut(s) 303, 886, 1186, 1465
BmrI ACTGGG 1 cut(s) 737
BmsI GCATC 5 cut(s) 163, 394, 898, 1105, 1285
BmuI ACTGGG 1 cut(s) 737
BpmI CTGGAG 2 cut(s) 456, 1239
BpuEI CTTGAG 2 cut(s) 474, 685
BpuMI CCSGG 2 cut(s) 303, 1186
BsaAI YACGTR 1 cut(s) 1665
BsaHI GRCGYC 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 1473
BsaJI CCNNGG 2 cut(s) 810, 1049
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 419, 861, 1054
Bse1I ACTGG 5 cut(s) 313, 473, 743, 768, 1078
Bse3DI GCAATG 1 cut(s) 1142
BseBI CCWGG 2 cut(s) 886, 1465
BseDI CCNNGG 2 cut(s) 810, 1049
BseGI GGATG 5 cut(s) 436, 784, 889, 1120, 1194
BseLI CCNNNNNNNGG 4 cut(s) 308, 419, 861, 1054
BseMI GCAATG 1 cut(s) 1142
BseMII CTCAG 4 cut(s) 221, 1379, 1454, 1568
BseNI ACTGG 5 cut(s) 313, 473, 743, 768, 1078
BseRI GAGGAG 3 cut(s) 104, 107, 241
BseSI GKGCMC 1 cut(s) 743
BseXI GCAGC 2 cut(s) 77, 1375
BsgI GTGCAG 1 cut(s) 355
Bsh1236I CGCG 2 cut(s) 165, 189
BshFI GGCC 3 cut(s) 423, 871, 1463
BshNI GGYRCC 1 cut(s) 1496
BsiHKAI GWGCWC 3 cut(s) 10, 490, 1570
BsiSI CCGG 2 cut(s) 303, 1185
BslFI GGGAC 3 cut(s) 557, 610, 1363
BslI CCNNNNNNNGG 4 cut(s) 308, 419, 861, 1054
BsmAI GTCTC 3 cut(s) 196, 1103, 1473
BsmBI CGTCTC 2 cut(s) 196, 1103
BsmFI GGGAC 3 cut(s) 557, 610, 1363
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 3 cut(s) 423, 871, 1463
Bso31I GGTCTC 1 cut(s) 1473
Bsp1286I GDGCHC 4 cut(s) 10, 490, 743, 1570
Bsp143I GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
BspACI CCGC 7 cut(s) 51, 54, 86, 163, 187, 1118, 1500
BspANI GGCC 3 cut(s) 423, 871, 1463
BspCNI CTCAG 4 cut(s) 220, 1380, 1453, 1567
BspFNI CGCG 2 cut(s) 165, 189
BspLI GGNNCC 2 cut(s) 1498, 1601
BspPI GGATC 3 cut(s) 1031, 1499, 1507
BspQI GCTCTTC 1 cut(s) 1255
BspT107I GGYRCC 1 cut(s) 1496
BspTNI GGTCTC 1 cut(s) 1473
BsrDI GCAATG 1 cut(s) 1142
BsrI ACTGG 5 cut(s) 313, 473, 743, 768, 1078
BssECI CCNNGG 2 cut(s) 810, 1049
BssMI GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 2 cut(s) 810, 1049
Bst2UI CCWGG 2 cut(s) 886, 1465
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 2 cut(s) 1255, 1337
BstACI GRCGYC 1 cut(s) 268
BstBAI YACGTR 1 cut(s) 1665
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 5 cut(s) 207, 833, 1388, 1440, 1554
BstF5I GGATG 5 cut(s) 436, 784, 889, 1120, 1194
BstFNI CGCG 2 cut(s) 165, 189
BstKTI GATC 9 cut(s) 150, 341, 754, 961, 1026, 1087, 1455, 1507, 1515
BstMAI GTCTC 3 cut(s) 196, 1103, 1473
BstMBI GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
BstMWI GCNNNNNNNGC 3 cut(s) 140, 687, 1360
BstNI CCWGG 2 cut(s) 886, 1465
BstSCI CCNGG 4 cut(s) 301, 884, 1184, 1463
BstSLI GKGCMC 1 cut(s) 743
BstUI CGCG 2 cut(s) 165, 189
BstV1I GCAGC 2 cut(s) 77, 1375
BstX2I RGATCY 2 cut(s) 958, 1504
BstXI CCANNNNNNTGG 2 cut(s) 775, 1412
BstYI RGATCY 2 cut(s) 958, 1504
BsuI GTATCC 2 cut(s) 862, 1051
BsuRI GGCC 3 cut(s) 423, 871, 1463
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 5 cut(s) 436, 784, 889, 1120, 1194
BtsI GCAGTG 1 cut(s) 1438
BtsIMutI CAGTG 4 cut(s) 750, 775, 1071, 1438
Cac8I GCNNGC 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 4 cut(s) 178, 276, 1256, 1360
Csp6I GTAC 2 cut(s) 306, 1497
CviAII CATG 8 cut(s) 666, 1013, 1088, 1297, 1336, 1355, 1406, 1608
CviQI GTAC 2 cut(s) 306, 1497
DdeI CTNAG 5 cut(s) 207, 833, 1388, 1440, 1554
DpnI GATC 9 cut(s) 149, 340, 753, 960, 1025, 1086, 1454, 1506, 1514
DpnII GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 2 cut(s) 1255, 1337
EarI CTCTTC 2 cut(s) 1255, 1337
EciI GGCGGA 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 810, 1049
Eco147I AGGCCT 1 cut(s) 1463
Eco31I GGTCTC 1 cut(s) 1473
Eco32I GATATC 2 cut(s) 1381, 1528
Eco57I CTGAAG 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 884, 1463
EcoRV GATATC 2 cut(s) 1381, 1528
EcoT14I CCWWGG 2 cut(s) 810, 1049
EcoT22I ATGCAT 2 cut(s) 1161, 1298
ErhI CCWWGG 2 cut(s) 810, 1049
Esp3I CGTCTC 2 cut(s) 196, 1103
FaeI CATG 8 cut(s) 669, 1016, 1091, 1300, 1339, 1358, 1409, 1611
FalI AAGNNNNNCTT 4 cut(s) 120, 152, 718, 750
FaqI GGGAC 3 cut(s) 557, 610, 1363
FatI CATG 8 cut(s) 665, 1012, 1087, 1296, 1335, 1354, 1405, 1607
FauI CCCGC 1 cut(s) 170
FbaI TGATCA 3 cut(s) 751, 1084, 1452
FblI GTMKAC 1 cut(s) 1651
Fnu4HI GCNGC 4 cut(s) 52, 55, 66, 1364
FokI GGATG 5 cut(s) 423, 791, 876, 1127, 1201
Fsp4HI GCNGC 4 cut(s) 52, 55, 66, 1364
FspBI CTAG 8 cut(s) 291, 375, 459, 483, 510, 654, 819, 956
GluI GCNGC 4 cut(s) 52, 55, 66, 1364
GsuI CTGGAG 2 cut(s) 456, 1239
HaeIII GGCC 3 cut(s) 423, 871, 1463
HapII CCGG 2 cut(s) 303, 1185
HgaI GACGC 4 cut(s) 178, 276, 1256, 1360
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 8 cut(s) 669, 1016, 1091, 1300, 1339, 1358, 1409, 1611
HincII GTYRAC 3 cut(s) 234, 288, 790
HindII GTYRAC 3 cut(s) 234, 288, 790
HinfI GANTC 2 cut(s) 938, 1436
HpaI GTTAAC 1 cut(s) 234
HpaII CCGG 2 cut(s) 303, 1185
HphI GGTGA 3 cut(s) 88, 291, 947
Hpy166II GTNNAC 7 cut(s) 172, 234, 288, 299, 649, 790, 1652
Hpy188I TCNGA 9 cut(s) 12, 147, 1281, 1370, 1385, 1452, 1532, 1575, 1636
Hpy188III TCNNGA 6 cut(s) 375, 587, 893, 956, 1102, 1472
Hpy8I GTNNAC 7 cut(s) 172, 234, 288, 299, 649, 790, 1652
Hpy99I CGWCG 2 cut(s) 270, 1478
HpyAV CCTTC 4 cut(s) 188, 359, 1560, 1625
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 2 cut(s) 33, 1664
HpyCH4V TGCA 5 cut(s) 336, 686, 1147, 1159, 1296
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 687, 1360
HpyF3I CTNAG 5 cut(s) 207, 833, 1388, 1440, 1554
HpySE526I ACGT 2 cut(s) 33, 1664
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 8 cut(s) 669, 1016, 1091, 1300, 1339, 1358, 1409, 1611
KpnI GGTACC 1 cut(s) 1500
Ksp22I TGATCA 3 cut(s) 751, 1084, 1452
KspAI GTTAAC 1 cut(s) 234
Kzo9I GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
LguI GCTCTTC 1 cut(s) 1255
LmnI GCTCC 5 cut(s) 121, 475, 1220, 1371, 1605
Lsp1109I GCAGC 2 cut(s) 77, 1375
LweI GCATC 5 cut(s) 163, 394, 898, 1105, 1285
MaeI CTAG 8 cut(s) 291, 375, 459, 483, 510, 654, 819, 956
MaeII ACGT 2 cut(s) 33, 1664
MaeIII GTNAC 5 cut(s) 692, 763, 935, 988, 1069
MalI GATC 9 cut(s) 149, 340, 753, 960, 1025, 1086, 1454, 1506, 1514
MboI GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
MflI RGATCY 2 cut(s) 958, 1504
MhlI GDGCHC 4 cut(s) 10, 490, 743, 1570
MlsI TGGCCA 1 cut(s) 871
MluCI AATT 5 cut(s) 246, 559, 608, 632, 1233
MluNI TGGCCA 1 cut(s) 871
MlyI GAGTC 1 cut(s) 932
MmeI TCCRAC 1 cut(s) 1393
MnlI CCTC 9 cut(s) 22, 82, 85, 219, 222, 1013, 1020, 1048, 1383
Mox20I TGGCCA 1 cut(s) 871
Mph1103I ATGCAT 2 cut(s) 1161, 1298
MroXI GAANNNNTTC 2 cut(s) 562, 730
MscI TGGCCA 1 cut(s) 871
MseI TTAA 5 cut(s) 233, 249, 1029, 1272, 1326
MslI CAYNNNNRTG 2 cut(s) 360, 894
Msp20I TGGCCA 1 cut(s) 871
MspA1I CMGCKG 1 cut(s) 1217
MspI CCGG 2 cut(s) 303, 1185
MspR9I CCNGG 4 cut(s) 303, 886, 1186, 1465
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 2 cut(s) 886, 1465
MvnI CGCG 2 cut(s) 165, 189
MwoI GCNNNNNNNGC 3 cut(s) 140, 687, 1360
NciI CCSGG 2 cut(s) 303, 1186
NdeII GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
NlaIII CATG 8 cut(s) 669, 1016, 1091, 1300, 1339, 1358, 1409, 1611
NlaIV GGNNCC 2 cut(s) 1498, 1601
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 4 cut(s) 692, 763, 935, 1069
NsiI ATGCAT 2 cut(s) 1161, 1298
PceI AGGCCT 1 cut(s) 1463
PciSI GCTCTTC 1 cut(s) 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 1159
PdmI GAANNNNTTC 2 cut(s) 562, 730
PfeI GAWTC 1 cut(s) 1436
PkrI GCNGC 4 cut(s) 53, 56, 67, 1365
PleI GAGTC 1 cut(s) 932
PpsI GAGTC 1 cut(s) 932
Ppu21I YACGTR 1 cut(s) 1665
PsiI TTATAA 1 cut(s) 1097
Psp6I CCWGG 2 cut(s) 884, 1463
PspGI CCWGG 2 cut(s) 884, 1463
PspN4I GGNNCC 2 cut(s) 1498, 1601
PspPI GGNCC 1 cut(s) 422
PsuI RGATCY 2 cut(s) 958, 1504
PvuII CAGCTG 1 cut(s) 1217
RsaI GTAC 2 cut(s) 307, 1498
RsaNI GTAC 2 cut(s) 306, 1497
RseI CAYNNNNRTG 2 cut(s) 360, 894
SapI GCTCTTC 1 cut(s) 1255
SaqAI TTAA 5 cut(s) 233, 249, 1029, 1272, 1326
SatI GCNGC 4 cut(s) 52, 55, 66, 1364
Sau3AI GATC 9 cut(s) 147, 338, 751, 958, 1023, 1084, 1452, 1504, 1512
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 1 cut(s) 932
ScrFI CCNGG 4 cut(s) 303, 886, 1186, 1465
SduI GDGCHC 4 cut(s) 10, 490, 743, 1570
SfaNI GCATC 5 cut(s) 163, 394, 898, 1105, 1285
SmiMI CAYNNNNRTG 2 cut(s) 360, 894
SmlI CTYRAG 2 cut(s) 489, 700
SmoI CTYRAG 2 cut(s) 489, 700
Sse9I AATT 5 cut(s) 246, 559, 608, 632, 1233
SseBI AGGCCT 1 cut(s) 1463
SsiI CCGC 7 cut(s) 51, 54, 86, 163, 187, 1118, 1500
SspMI CTAG 8 cut(s) 291, 375, 459, 483, 510, 654, 819, 956
StuI AGGCCT 1 cut(s) 1463
StyD4I CCNGG 4 cut(s) 301, 884, 1184, 1463
StyI CCWWGG 2 cut(s) 810, 1049
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 2 cut(s) 36, 1667
TaqI TCGA 4 cut(s) 150, 259, 265, 1524
TasI AATT 5 cut(s) 246, 559, 608, 632, 1233
TauI GCSGC 2 cut(s) 54, 57
TfiI GAWTC 1 cut(s) 1436
Tru1I TTAA 5 cut(s) 233, 249, 1029, 1272, 1326
Tru9I TTAA 5 cut(s) 233, 249, 1029, 1272, 1326
TscAI CASTG 4 cut(s) 750, 775, 1078, 1438
TseFI GTSAC 4 cut(s) 692, 763, 935, 1069
TseI GCWGC 2 cut(s) 65, 1363
Tsp45I GTSAC 4 cut(s) 692, 763, 935, 1069
TspDTI ATGAA 5 cut(s) 378, 397, 551, 1152, 1324
TspGWI ACGGA 3 cut(s) 41, 619, 1127
TspRI CASTG 4 cut(s) 750, 775, 1078, 1438
XapI RAATTY 2 cut(s) 559, 608
XbaI TCTAGA 2 cut(s) 374, 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 864
XmiI GTMKAC 1 cut(s) 1651
XmnI GAANNNNTTC 2 cut(s) 562, 730
XspI CTAG 8 cut(s) 291, 375, 459, 483, 510, 654, 819, 956
Zsp2I ATGCAT 2 cut(s) 1161, 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.