RchiOBHm_Chr7g0188721

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007591
Physical Location & Seq
Reverse (-)
8114290 .. 8119025
4736 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16853

Sequence Viewer

Length: 1614 bp
ATGAGTGCTCTTCTCAAAAACCCCAGAAGAGAAAGCGTGGAGATTGACGACGACGACACCATGATGCCGCCGGCGGAAGAGAACGGCGACCCAGATAGCAGACAAGACCAAGAAGACGCCCTCATCGCTCTGATTGAGCACCGCGCTCAGGAAGTGGAACACAATCGCCGGAGACGGGAGTATTACCAATCCAAGCTTGTTGAAGCAGAGAAGAGGTTGAATGAGTCTCAAACTAAATTGGCTCGACTTCGATCCCAAAACAAGGCAGCGTCGACTAAGGTGTCTCCGAGTAATGGAATTAAAAGTGTAAAGGAGGAGCGCAGATCAATGAGTCCTATCCCTTTAAGAAGCCGGCCTCAATCTAAAACAGAACTTTTAATTCCTGCTGTCACTCCAAAAGTTTCCCAACCTCCGAAAGTGGCAGGGTCTAGTGGGAAGGCACAAGTTAGTTCTAGTTCTCAAGCCAGCCCGGCAGTTTCTAGTCAGTCTATTAGTGCTAGCAAAGAAAAAGGAGATAAGTCTTATAGAAGTTCGTCAAAGCAGGATGTTGAAGTTCAAGATAAAGGGACAAAACGGAAGTTCGAGGAGAAAGAACATAAAGAATTAATTCCGATGGTTCGTACTAGCTCCAAACCCTCTATAATCCAGTGCCATTCAAACAATCACATATCCAGTCATCACAAGAGAAAGTTGAGATGTCTTTATATGTGTCCAGTGAATGATCAGCTTTTTGCAACCAGCGCTTTGGATGGACTGGTCAACTTGTGGGAAGTTCAAGGCAGGGGGTCAGGTGCTTCTCTACTTAGTACTACAGATTGCCAATCCCCAAAGCAAAGGAGATGGCCAGAAGATATTGCCTGGCATCCCCATGGAAACAGCCTATTTTCTGTGTATACTGCTGATGGTGGGGAATCACAAGTATCAGTTCTAAATCTAAATCGAACACAAGGGAAACCTCGTGTTACTTTCTTGGAAGCCAAGCCTCATGTGAAAGGGATTATTAACAGCATAGTTTTCCCACCTTGGGAGAATACCTGCTTTGTCACTGGTGGTAGTGATCATGCTGTTGTACTCTGGAAAGAGACGGATGCAGAAAATGTCTGGAAACCAAAGCAATTGCATAGGAGTATACATTCATCTGCTGTTATGGGCGTTGCTGGGATGTGGCAAAAGCAAATTATACTATCTGTGGGAGCAGACAAGAGGATTGTTGGCTTCAATGCTAATATGGAAAGAACAGAGTTTAAGCATCAGATAGATAGTAAATGCATGAGTGTTTTGCCAAATCCTTGCGACTTTAATCTATTCATGGTTCAAGCAGGTACTCATGAGAAGCAGCTCCGACTATATGATATCAGACTGAGGCAAACAGAGATACATGCTTTTGGGTGGAAGCAAGAGAGCAGTGATTCTCAATCGGCTCTGATAAATCAAGCTTGGTCTCCTGATGGTTTATACATCTCATCCGGTACTGCTGATCCTATGATTCACATATTTGATATCAGGTATAACGCCCAGAAGCCTTCCCAATCCATTAAGGCTCATCAGAAACGGGTTTTCAAAGCTGTATGGCTCTGCACTAGCCCCTTCTCATTTCTATATCCTCTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

537

Amino Acids

60.37

Weight (kDa)

9.31

Isoelectric Point (pI)

66.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 222 - 403 1.2e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 225 - 520 7.9e-10 WDR5 beta-propeller domain
WD40_Prp19 PF24814 334 - 519 9.6e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 356 - 525 4.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 378 - 524 1e-06 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 383 - 524 4.6e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
Acc36I ACCTGC 2 cut(s) 1043, 1310
AccI GTMKAC 3 cut(s) 272, 893, 1129
AccII CGCG 1 cut(s) 144
AciI CCGC 3 cut(s) 68, 74, 142
AclWI GGATC 2 cut(s) 246, 1472
AcoI YGGCCR 1 cut(s) 842
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 5 cut(s) 622, 808, 1071, 1324, 1471
AfeI AGCGCT 1 cut(s) 742
AfiI CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
AgsI TTSAA 9 cut(s) 203, 220, 551, 557, 657, 776, 1219, 1316, 1561
AjnI CCWGG 1 cut(s) 857
AluBI AGCT 6 cut(s) 196, 627, 727, 1339, 1436, 1565
AluI AGCT 6 cut(s) 196, 627, 727, 1339, 1436, 1565
Alw21I GWGCWC 2 cut(s) 10, 141
Alw26I GTCTC 5 cut(s) 166, 231, 288, 1076, 1446
AlwI GGATC 2 cut(s) 246, 1472
Aor51HI AGCGCT 1 cut(s) 742
AoxI GGCC 2 cut(s) 353, 842
ApeKI GCWGC 2 cut(s) 266, 1336
AseI ATTAAT 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 3 cut(s) 146, 321, 743
AsuC2I CCSGG 1 cut(s) 470
AsuNHI GCTAGC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 844
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 10, 141
BbvI GCAGC 2 cut(s) 278, 1348
BccI CCATC 5 cut(s) 607, 743, 834, 896, 1442
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 859
BclI TGATCA 2 cut(s) 721, 1057
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 5 cut(s) 166, 231, 288, 1076, 1446
BfaI CTAG 6 cut(s) 429, 453, 480, 498, 624, 1581
BfmI CTRYAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 744
BfuAI ACCTGC 2 cut(s) 1043, 1310
BglI GCCNNNNNGGC 1 cut(s) 470
BisI GCNGC 3 cut(s) 68, 267, 1337
BlsI GCNGC 3 cut(s) 69, 268, 1338
BmcAI AGTACT 1 cut(s) 808
Bme1390I CCNGG 2 cut(s) 470, 859
BmrFI CCNGG 2 cut(s) 470, 859
BmsI GCATC 4 cut(s) 54, 871, 1078, 1258
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 1446
BsaJI CCNNGG 2 cut(s) 868, 1022
BsaWI WCCGGW 1 cut(s) 1466
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
Bse118I RCCGGY 2 cut(s) 70, 351
Bse1I ACTGG 5 cut(s) 646, 672, 713, 759, 1051
BseBI CCWGG 1 cut(s) 859
BseDI CCNNGG 2 cut(s) 868, 1022
BseGI GGATG 6 cut(s) 550, 754, 862, 1093, 1167, 1463
BseLI CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
BseMII CTCAG 2 cut(s) 161, 1352
BseNI ACTGG 5 cut(s) 646, 672, 713, 759, 1051
BseRI GAGGAG 2 cut(s) 329, 599
BseXI GCAGC 2 cut(s) 278, 1348
BseYI CCCAGC 1 cut(s) 1157
BsgI GTGCAG 1 cut(s) 1561
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 355, 844
BsiHKAI GWGCWC 2 cut(s) 10, 141
BsiSI CCGG 5 cut(s) 71, 169, 352, 470, 1467
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 5 cut(s) 148, 175, 262, 293, 1024
BsmAI GTCTC 5 cut(s) 166, 231, 288, 1076, 1446
BsmBI CGTCTC 2 cut(s) 166, 1076
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 2 cut(s) 355, 844
Bso31I GGTCTC 1 cut(s) 1446
Bsp1286I GDGCHC 2 cut(s) 10, 141
Bsp143I GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
Bsp19I CCATGG 1 cut(s) 868
BspACI CCGC 3 cut(s) 68, 74, 142
BspANI GGCC 2 cut(s) 355, 844
BspCNI CTCAG 2 cut(s) 160, 1353
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 1327
BspMI ACCTGC 2 cut(s) 1043, 1310
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 2 cut(s) 246, 1472
BspQI GCTCTTC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 1446
BsrFI RCCGGY 2 cut(s) 70, 351
BsrI ACTGG 5 cut(s) 646, 672, 713, 759, 1051
BssAI RCCGGY 2 cut(s) 70, 351
BssECI CCNNGG 2 cut(s) 868, 1022
BssMI GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
BssNAI GTATAC 2 cut(s) 894, 1130
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 2 cut(s) 868, 1022
Bst1107I GTATAC 2 cut(s) 894, 1130
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 859
Bst6I CTCTTC 4 cut(s) 15, 22, 72, 206
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 4 cut(s) 72, 353, 466, 499
BstDEI CTNAG 4 cut(s) 147, 276, 803, 1361
BstDSI CCRYGG 1 cut(s) 868
BstF5I GGATG 6 cut(s) 550, 754, 862, 1093, 1167, 1463
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 744
BstHHI GCGC 3 cut(s) 146, 321, 743
BstKTI GATC 6 cut(s) 254, 326, 724, 1060, 1480, 1611
BstMAI GTCTC 5 cut(s) 166, 231, 288, 1076, 1446
BstMBI GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
BstMWI GCNNNNNNNGC 3 cut(s) 125, 470, 740
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 1 cut(s) 1382
BstSCI CCNGG 2 cut(s) 468, 857
BstSFI CTRYAG 1 cut(s) 810
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 278, 1348
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 745
BstZ17I GTATAC 2 cut(s) 894, 1130
BsuRI GGCC 2 cut(s) 355, 844
BtgI CCRYGG 1 cut(s) 868
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 6 cut(s) 550, 754, 862, 1093, 1167, 1463
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 4 cut(s) 653, 720, 1044, 1411
BveI ACCTGC 2 cut(s) 1043, 1310
Cac8I GCNNGC 4 cut(s) 72, 353, 466, 499
CciI TCATGA 1 cut(s) 1327
CfoI GCGC 3 cut(s) 146, 321, 743
Cfr10I RCCGGY 2 cut(s) 70, 351
CseI GACGC 2 cut(s) 125, 258
Csp6I GTAC 5 cut(s) 621, 807, 1070, 1323, 1470
CviAII CATG 8 cut(s) 61, 869, 986, 1061, 1270, 1309, 1328, 1379
CviQI GTAC 5 cut(s) 621, 807, 1070, 1323, 1470
DdeI CTNAG 4 cut(s) 147, 276, 803, 1361
DpnI GATC 6 cut(s) 253, 325, 723, 1059, 1479, 1610
DpnII GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 842
Eam1104I CTCTTC 4 cut(s) 15, 22, 72, 206
EarI CTCTTC 4 cut(s) 15, 22, 72, 206
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 868, 1022
Eco31I GGTCTC 1 cut(s) 1446
Eco32I GATATC 2 cut(s) 1354, 1501
Eco47III AGCGCT 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 857
EcoRV GATATC 2 cut(s) 1354, 1501
EcoT14I CCWWGG 2 cut(s) 868, 1022
EcoT22I ATGCAT 1 cut(s) 1271
ErhI CCWWGG 2 cut(s) 868, 1022
Esp3I CGTCTC 2 cut(s) 166, 1076
FaeI CATG 8 cut(s) 64, 872, 989, 1064, 1273, 1312, 1331, 1382
FaqI GGGAC 1 cut(s) 580
FatI CATG 8 cut(s) 60, 868, 985, 1060, 1269, 1308, 1327, 1378
FbaI TGATCA 2 cut(s) 721, 1057
FblI GTMKAC 3 cut(s) 272, 893, 1129
Fnu4HI GCNGC 3 cut(s) 68, 267, 1337
FokI GGATG 6 cut(s) 557, 761, 849, 1100, 1174, 1450
Fsp4HI GCNGC 3 cut(s) 68, 267, 1337
FspBI CTAG 6 cut(s) 429, 453, 480, 498, 624, 1581
GlaI GCGC 3 cut(s) 145, 320, 742
GluI GCNGC 3 cut(s) 68, 267, 1337
GsaI CCCAGC 1 cut(s) 1161
HaeII RGCGCY 1 cut(s) 744
HaeIII GGCC 2 cut(s) 355, 844
HapII CCGG 5 cut(s) 71, 169, 352, 470, 1467
HgaI GACGC 2 cut(s) 125, 258
HhaI GCGC 3 cut(s) 146, 321, 743
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 8 cut(s) 64, 872, 989, 1064, 1273, 1312, 1331, 1382
Hin6I GCGC 3 cut(s) 144, 319, 741
HinP1I GCGC 3 cut(s) 144, 319, 741
HincII GTYRAC 2 cut(s) 273, 760
HindII GTYRAC 2 cut(s) 273, 760
HindIII AAGCTT 2 cut(s) 194, 1434
HinfI GANTC 5 cut(s) 224, 331, 911, 1409, 1486
HpaII CCGG 5 cut(s) 71, 169, 352, 470, 1467
Hpy166II GTNNAC 4 cut(s) 273, 760, 894, 1130
Hpy188III TCNNGA 6 cut(s) 149, 557, 1075, 1102, 1328, 1445
Hpy8I GTNNAC 4 cut(s) 273, 760, 894, 1130
Hpy99I CGWCG 3 cut(s) 53, 56, 274
HpyAV CCTTC 3 cut(s) 430, 1533, 1597
HpyCH4V TGCA 5 cut(s) 734, 1091, 1120, 1269, 1578
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 470, 740
HpyF3I CTNAG 4 cut(s) 147, 276, 803, 1361
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 8 cut(s) 64, 872, 989, 1064, 1273, 1312, 1331, 1382
HspAI GCGC 3 cut(s) 144, 319, 741
KroI GCCGGC 2 cut(s) 70, 351
KroNI GCCGGC 2 cut(s) 72, 353
Ksp22I TGATCA 2 cut(s) 721, 1057
Kzo9I GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 4 cut(s) 316, 632, 1193, 1344
Lsp1109I GCAGC 2 cut(s) 278, 1348
LweI GCATC 4 cut(s) 54, 871, 1078, 1258
MaeI CTAG 6 cut(s) 429, 453, 480, 498, 624, 1581
MaeIII GTNAC 3 cut(s) 388, 961, 1042
MalI GATC 6 cut(s) 253, 325, 723, 1059, 1479, 1610
MboI GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
MboII GAAGA 5 cut(s) 39, 89, 125, 223, 860
MfeI CAATTG 1 cut(s) 1115
MhlI GDGCHC 2 cut(s) 10, 141
MlsI TGGCCA 1 cut(s) 844
MluCI AATT 7 cut(s) 236, 297, 378, 602, 606, 1115, 1176
MluNI TGGCCA 1 cut(s) 844
MlyI GAGTC 2 cut(s) 233, 340
MmeI TCCRAC 1 cut(s) 1366
Mox20I TGGCCA 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 1271
MreI CGCCGGCG 1 cut(s) 70
MroNI GCCGGC 2 cut(s) 70, 351
MroXI GAANNNNTTC 1 cut(s) 606
MscI TGGCCA 1 cut(s) 844
MseI TTAA 8 cut(s) 300, 344, 377, 605, 1002, 1245, 1299, 1536
MslI CAYNNNNRTG 2 cut(s) 62, 867
Msp20I TGGCCA 1 cut(s) 844
MspI CCGG 5 cut(s) 71, 169, 352, 470, 1467
MspR9I CCNGG 2 cut(s) 470, 859
MunI CAATTG 1 cut(s) 1115
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 125, 470, 740
NaeI GCCGGC 2 cut(s) 72, 353
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 868
NdeII GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
NgoMIV GCCGGC 2 cut(s) 70, 351
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 8 cut(s) 64, 872, 989, 1064, 1273, 1312, 1331, 1382
NmuCI GTSAC 2 cut(s) 388, 1042
NsiI ATGCAT 1 cut(s) 1271
NspI RCATGY 1 cut(s) 1382
PagI TCATGA 1 cut(s) 1327
PciSI GCTCTTC 1 cut(s) 15
PdiI GCCGGC 2 cut(s) 72, 353
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 3 cut(s) 911, 1409, 1486
PkrI GCNGC 3 cut(s) 69, 268, 1338
PleI GAGTC 2 cut(s) 232, 339
PpsI GAGTC 2 cut(s) 232, 339
PshBI ATTAAT 1 cut(s) 605
Psp6I CCWGG 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 1 cut(s) 857
RsaI GTAC 5 cut(s) 622, 808, 1071, 1324, 1471
RsaNI GTAC 5 cut(s) 621, 807, 1070, 1323, 1470
RseI CAYNNNNRTG 2 cut(s) 62, 867
SalI GTCGAC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 8 cut(s) 300, 344, 377, 605, 1002, 1245, 1299, 1536
SatI GCNGC 3 cut(s) 68, 267, 1337
Sau3AI GATC 6 cut(s) 251, 323, 721, 1057, 1477, 1608
ScaI AGTACT 1 cut(s) 808
SchI GAGTC 2 cut(s) 233, 340
ScrFI CCNGG 2 cut(s) 470, 859
SduI GDGCHC 2 cut(s) 10, 141
SfaNI GCATC 4 cut(s) 54, 871, 1078, 1258
SfcI CTRYAG 1 cut(s) 810
SgrAI CRCCGGYG 1 cut(s) 70
SmiMI CAYNNNNRTG 2 cut(s) 62, 867
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 7 cut(s) 236, 297, 378, 602, 606, 1115, 1176
SsiI CCGC 3 cut(s) 68, 74, 142
SspMI CTAG 6 cut(s) 429, 453, 480, 498, 624, 1581
StyD4I CCNGG 2 cut(s) 468, 857
StyI CCWWGG 2 cut(s) 868, 1022
TaqI TCGA 5 cut(s) 244, 250, 272, 582, 940
TasI AATT 7 cut(s) 236, 297, 378, 602, 606, 1115, 1176
TatI WGTACW 2 cut(s) 806, 1069
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 3 cut(s) 911, 1409, 1486
Tru1I TTAA 8 cut(s) 300, 344, 377, 605, 1002, 1245, 1299, 1536
Tru9I TTAA 8 cut(s) 300, 344, 377, 605, 1002, 1245, 1299, 1536
TscAI CASTG 4 cut(s) 653, 720, 1051, 1411
TseFI GTSAC 2 cut(s) 388, 1042
TseI GCWGC 2 cut(s) 266, 1336
Tsp45I GTSAC 2 cut(s) 388, 1042
TspDTI ATGAA 2 cut(s) 1125, 1297
TspGWI ACGGA 2 cut(s) 589, 1100
TspRI CASTG 4 cut(s) 653, 720, 1051, 1411
VspI ATTAAT 1 cut(s) 605
XceI RCATGY 1 cut(s) 1382
XmiI GTMKAC 3 cut(s) 272, 893, 1129
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 6 cut(s) 429, 453, 480, 498, 624, 1581
ZrmI AGTACT 1 cut(s) 808
Zsp2I ATGCAT 1 cut(s) 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.