RLG00000004667

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007591
Physical Location & Seq
Forward (+)
61285408 .. 61294012
8605 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004667

Sequence Viewer

Length: 2850 bp
ATGAGTGCTGTTCTCAAAAACCCCAGAAGAGAAAGCGTGGCGATTGACGACGACGTCGACAACACCATGATGCCGCCGGCGGTAGAGAACGGCGACCCAGATAGCAGACAAGACCAAGAAGACGCCCTCATCGCTCTGATTGAGCACCGCGCTCAGGAAGTGGAACACAATCGCCGGAGACGGGAGTATTACCAATCCAAGCTTGTTGAAGCAGAGAAGAGGTTGAATGAATCTCAAACTAAATTGGCTCGACTTCGATTCCAAAACAAGGCAGTGTCGACTAAGGCGTCTCCGAGTAATGGAATTAAAAGTGTAAAGGAGGAGCGCAGATCAATAAGTCCGATCCCTTTAAGAAGCCAGCCTCAATCTAAAACAGAACTTTTAATTCCTGCTGTCACTCCAAAAGTTTCCCAATCCCCGAAAGTGGCAGGGTCTAGTGGGAAGGCACAAGTTAGTTCTAGTTCTCAAGCCAGCCCTGCAGTTTCTAGTCAGTCTATTACTGCTAGCAAAGAAAAAGGAGATAAGTCTTATAGAAGTTCGTCAAAGCAGGATGTTGAAGTTCAAGATAAAGGGACAAAACGAAAGTTCGAGGAGAAAGAACATAAAGAATTAATTCCGATGGTTCGTACAAGCTCCAAACCCTCTATAATCCAGTGCCATTCAAACAATCACATATCCAGTCATCACAAGAGAAAGCTGAGATGTCTTTATTTGTGTCCAGTAAATGATCAGCTTTTTGCAACCAGCGCTTTGGATGGACTGGTCAACTTGTGGGAAGTTCAAGGCAGGGGGTCAGGTGCTTCTCTACTTAGTACTACAGACTGCCAATCCCCAAAGCAAAGGAGATGGCCAGAAGATATTGCCTGGCATCCCCATGGAAACAGCCTATTTTCTGTGTATACTGCTGATGGTGGGGAATCACAAGTATCAGTTCTAAATCTAAATCGAACACAAGGGAAACCTCGTGTTACTTTCTTGGAAGCCAAGCCTCACGTGAAAGGGATTATTAACAGCATAGTTTTCCCACCTTGGGAGAATACCTGCTTCGTCACTGGTGGTAGCGATCATGCTGTTGTACTCTGGAAAGAGACAGATGCAGAAAATGTCTGGAAACCAAAGCAATTGCATAGGAGTATACATTCATCTGCTGTTATGGGCGTTGCTGGGATGTGGCAAAAGCAAATTATACTATCTGTGGGAGCAGACAAGAGGATTGTTGGCTTCGATGCTAATATGGAAAGAACGGAGTTTAAGCATCAGATAGATAGTAAATGCATGAGTGTTTTGCCAAATCCTTGCGACTTTAATCTATTCATGGTTCAAGCAGGTACTCATGAGAAGCAGCTCCGACTTTATGATATCAGACTGAGGCAAACAGAGATACATGCTTTTGGGTGGAAGCAAGAGAGCAGTGATTCTCAATCGGCTCTGATAAATCAAGCATGGTCTCCTGATGGTTTATACATCTCATCCGGTACTGCTGATCCTATGATTCACATATTTGATATCAGGTATAACGCCCAGAAGCCTTCCCAATCCATTAAGGCTCATCAGAAACGGGTTTTCAAAGCTGTATGGCTCCGCACTAGCCCCCTTCTCATTTCTATATCCTCTGATCTGATGTTTTTGGCACTGAAACTTCGTCTCCAAGTCTTCAACATGAACTGTGGAGGAAATGAGGACTTGGAATATCGAGCATCGGCTGTAGGTGACTTGAATGCAAATTATGTTACCCCTTTGCCCCCTATGAGTCGTTGGTATGGTCATATTTCTGCTCCTGCCGATATCATAAGTAGAGATGGCGCAAAGGAACAAATGGTGTGCAACAACCAGATGAATTCGGAGGATTTTTCTGGTTATATTTTTATGTGTAATGGACAAACAAAACCAGAATGCTATATGTACCGTGTTTTTGGACTTCCTGCTGGAAGAAGGGAAGTTATTCAGAAGATTAAGCCAGGGATGAAGCTATTTCTATTTGACTATGATGTGAAGTATCTTTATGGGGTATACGAAGCAACCACTGCTGGAAAATTGAACTGGGAAACAGCTGCTTTTGGTGGGAACTTCCCTGCACAGGTGAGATTCCATATCTACAAGGAATGTCTCCCTCTGCCAGAGAGTTGTTTCAAACATGCTATTCGAGATAACTACCAGACAGGCTCCAAAAAATTTAATCCAATTCTCAGTAGCCAACAAGTAAGTCATCTATTGTCAATGTTCAATCCTCTTACAAAGCCGTCATCTGCTAAATATGACCATTACCCTGTAAAGAATCAATTTCAATCCCATTCAAGGCTTCCTCCTCCAAGCTATCCATACTTTAACTTGATAAACACCAGTCATGCTCCACAAGCGTCACTGCCTCCAAGCTATTCTTTCATGTATGGCAGACTTACTTCATCAGCCTTGGAGCCTCAGTATACTCAAATGAGTGTCCTGCAGCCAGAACATGTTGGTTATGGATATGCACCACAGACGGGCTACATTAATCCTGCAGTGCAACCGCAAGCCAATTCAGCTCCATATCAGACATCTTATACTGCAGAAGCTGGGCAGCCGTACTTGCATGAAGTACCCAAATCATATGAAAGGTATGAGGCTGCTGCACAAGCTATGGTTCCTACTCATCAATACAGTGACTGGAGATATGAGTACAATCAGTTACCGTCACAGACCCATATGCAGATGCAGGAGAATGCTGCTCAACACCAGAGTCTCTATCAAGTGCCTGCAGCCCATCATGTTACACAGCCTATACAACAACACGCATCGAGATTCAAAGAGCCTGGGCCTGCTGTGAGGATTCTTCCCATTTCATCATCAAATACCTGGACACTGCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

950

Amino Acids

106.93

Weight (kDa)

9.06

Isoelectric Point (pI)

56.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 224 - 411 2.4e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 329 - 520 1.8e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 385 - 546 8.1e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 391 - 539 2.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 443 - 539 1.1e-06 THOC3 beta-propeller domain
Dev_Cell_Death PF10539 619 - 741 1.3e-47 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 53, 286
AatII GACGTC 1 cut(s) 57
Acc36I ACCTGC 2 cut(s) 1049, 1316
AccI GTMKAC 6 cut(s) 57, 278, 899, 1135, 2010, 2423
AccII CGCG 1 cut(s) 150
AciI CCGC 5 cut(s) 74, 80, 148, 1582, 2507
AclWI GGATC 2 cut(s) 337, 1478
AcoI YGGCCR 1 cut(s) 848
AcsI RAATTY 3 cut(s) 1837, 2171, 2844
AcvI CACGTG 1 cut(s) 994
AcyI GRCGYC 3 cut(s) 54, 123, 287
AfaI GTAC 9 cut(s) 628, 814, 1077, 1330, 1477, 1904, 2564, 2577, 2657
AfeI AGCGCT 1 cut(s) 748
AfiI CCNNNNNNNGG 9 cut(s) 154, 181, 268, 299, 424, 1030, 2077, 2295, 2480
AflIII ACRYGT 1 cut(s) 2452
AjnI CCWGG 4 cut(s) 863, 1957, 2788, 2831
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 7 cut(s) 172, 294, 1082, 1452, 1649, 2111, 2723
AlwI GGATC 2 cut(s) 337, 1478
AlwNI CAGNNNCTG 2 cut(s) 2552, 2643
Aor51HI AGCGCT 1 cut(s) 748
AoxI GGCC 2 cut(s) 848, 2792
ApeKI GCWGC 8 cut(s) 1342, 2051, 2443, 2557, 2603, 2606, 2702, 2735
ApoI RAATTY 3 cut(s) 1837, 2171, 2844
AseI ATTAAT 2 cut(s) 611, 2490
Asp700I GAANNNNTTC 2 cut(s) 612, 1941
AspLEI GCGC 4 cut(s) 152, 327, 749, 1805
AspS9I GGNCC 1 cut(s) 2792
AsuHPI GGTGA 2 cut(s) 1721, 2092
AsuNHI GCTAGC 1 cut(s) 503
BalI TGGCCA 1 cut(s) 850
BauI CACGAG 1 cut(s) 963
BbrPI CACGTG 1 cut(s) 994
BbsI GAAGAC 2 cut(s) 126, 1645
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 8 cut(s) 1354, 2038, 2455, 2569, 2590, 2593, 2689, 2747
BccI CCATC 7 cut(s) 613, 749, 840, 902, 1448, 1793, 2748
BceAI ACGGC 3 cut(s) 106, 2224, 2545
BciT130I CCWGG 4 cut(s) 865, 1959, 2790, 2833
BclI TGATCA 1 cut(s) 727
BcoDI GTCTC 7 cut(s) 172, 294, 1082, 1452, 1649, 2111, 2723
BfaI CTAG 5 cut(s) 435, 459, 486, 504, 1587
BfmI CTRYAG 7 cut(s) 477, 816, 1704, 2441, 2496, 2544, 2733
BfoI RGCGCY 1 cut(s) 750
BfuAI ACCTGC 2 cut(s) 1049, 1316
BisI GCNGC 9 cut(s) 74, 1343, 2052, 2444, 2558, 2604, 2607, 2703, 2736
BlsI GCNGC 9 cut(s) 75, 1344, 2053, 2445, 2559, 2605, 2608, 2704, 2737
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 4 cut(s) 865, 1959, 2790, 2833
BmgT120I GGNCC 1 cut(s) 2792
BmiI GGNNCC 4 cut(s) 1580, 2164, 2415, 2622
BmrFI CCNGG 4 cut(s) 865, 1959, 2790, 2833
BmrI ACTGGG 1 cut(s) 2050
BmsI GCATC 8 cut(s) 60, 877, 1084, 1216, 1264, 1706, 2679, 2780
BmtI GCTAGC 1 cut(s) 507
BmuI ACTGGG 1 cut(s) 2050
BpiI GAAGAC 2 cut(s) 126, 1645
BpmI CTGGAG 1 cut(s) 2665
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 994
BsaHI GRCGYC 3 cut(s) 54, 123, 287
BsaI GGTCTC 1 cut(s) 1452
BsaJI CCNNGG 5 cut(s) 874, 1028, 1958, 2409, 2789
BsaWI WCCGGW 1 cut(s) 1472
BsaXI ACNNNNNCTCC 4 cut(s) 1629, 1659, 2405, 2435
Bsc4I CCNNNNNNNGG 9 cut(s) 154, 181, 268, 299, 424, 1030, 2077, 2295, 2480
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 8 cut(s) 652, 678, 719, 765, 1057, 2045, 2340, 2648
BseBI CCWGG 4 cut(s) 865, 1959, 2790, 2833
BseDI CCNNGG 5 cut(s) 874, 1028, 1958, 2409, 2789
BseGI GGATG 6 cut(s) 556, 760, 868, 1173, 1469, 1968
BseLI CCNNNNNNNGG 9 cut(s) 154, 181, 268, 299, 424, 1030, 2077, 2295, 2480
BseMII CTCAG 5 cut(s) 167, 689, 1358, 2200, 2432
BseNI ACTGG 8 cut(s) 652, 678, 719, 765, 1057, 2045, 2340, 2648
BseRI GAGGAG 3 cut(s) 335, 605, 2295
BseXI GCAGC 8 cut(s) 1354, 2038, 2455, 2569, 2590, 2593, 2689, 2747
BseYI CCCAGC 2 cut(s) 1163, 2552
BsgI GTGCAG 2 cut(s) 2058, 2592
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 850, 2794
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 3 cut(s) 77, 175, 1473
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 9 cut(s) 154, 181, 268, 299, 424, 1030, 2077, 2295, 2480
BsmAI GTCTC 7 cut(s) 172, 294, 1082, 1452, 1649, 2111, 2723
BsmBI CGTCTC 3 cut(s) 172, 294, 1649
BsmFI GGGAC 1 cut(s) 586
BsmI GAATGC 3 cut(s) 1723, 1898, 2704
BsnI GGCC 2 cut(s) 850, 2794
Bso31I GGTCTC 1 cut(s) 1452
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
Bsp19I CCATGG 1 cut(s) 874
BspACI CCGC 5 cut(s) 74, 80, 148, 1582, 2507
BspANI GGCC 2 cut(s) 850, 2794
BspCNI CTCAG 5 cut(s) 166, 690, 1359, 2199, 2431
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 1333
BspLI GGNNCC 4 cut(s) 1580, 2164, 2415, 2622
BspMAI CTGCAG 5 cut(s) 481, 2445, 2500, 2548, 2737
BspMI ACCTGC 2 cut(s) 1049, 1316
BspOI GCTAGC 1 cut(s) 507
BspPI GGATC 2 cut(s) 337, 1478
BspTNI GGTCTC 1 cut(s) 1452
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 8 cut(s) 652, 678, 719, 765, 1057, 2045, 2340, 2648
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 5 cut(s) 874, 1028, 1958, 2409, 2789
BssMI GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
BssNAI GTATAC 4 cut(s) 900, 1136, 2011, 2424
BssNI GRCGYC 3 cut(s) 54, 123, 287
BssSI CACGAG 1 cut(s) 963
BssT1I CCWWGG 3 cut(s) 874, 1028, 2409
Bst1107I GTATAC 4 cut(s) 900, 1136, 2011, 2424
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 4 cut(s) 865, 1959, 2790, 2833
Bst4CI ACNGT 4 cut(s) 1667, 1907, 2639, 2670
Bst6I CTCTTC 2 cut(s) 22, 212
BstACI GRCGYC 3 cut(s) 54, 123, 287
BstAPI GCANNNNNTGC 1 cut(s) 2024
BstBAI YACGTR 1 cut(s) 994
BstC8I GCNNGC 7 cut(s) 78, 359, 472, 505, 2511, 2733, 2796
BstDEI CTNAG 7 cut(s) 153, 282, 698, 809, 1367, 2186, 2418
BstDSI CCRYGG 1 cut(s) 874
BstF5I GGATG 6 cut(s) 556, 760, 868, 1173, 1469, 1968
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 750
BstHHI GCGC 4 cut(s) 152, 327, 749, 1805
BstKTI GATC 6 cut(s) 332, 345, 730, 1066, 1486, 1618
BstMAI GTCTC 7 cut(s) 172, 294, 1082, 1452, 1649, 2111, 2723
BstMBI GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
BstMWI GCNNNNNNNGC 8 cut(s) 131, 476, 746, 2024, 2354, 2519, 2566, 2612
BstNI CCWGG 4 cut(s) 865, 1959, 2790, 2833
BstNSI RCATGY 3 cut(s) 1388, 2138, 2456
BstSCI CCNGG 4 cut(s) 863, 1957, 2788, 2831
BstSFI CTRYAG 7 cut(s) 477, 816, 1704, 2441, 2496, 2544, 2733
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 8 cut(s) 1354, 2038, 2455, 2569, 2590, 2593, 2689, 2747
BstV2I GAAGAC 2 cut(s) 126, 1645
BstXI CCANNNNNNTGG 1 cut(s) 751
BstZ17I GTATAC 4 cut(s) 900, 1136, 2011, 2424
BsuRI GGCC 2 cut(s) 850, 2794
BtgI CCRYGG 1 cut(s) 874
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 6 cut(s) 556, 760, 868, 1173, 1469, 1968
BtsI GCAGTG 6 cut(s) 279, 1417, 2022, 2360, 2505, 2837
BveI ACCTGC 2 cut(s) 1049, 1316
Cac8I GCNNGC 7 cut(s) 78, 359, 472, 505, 2511, 2733, 2796
CaiI CAGNNNCTG 2 cut(s) 2552, 2643
CciI TCATGA 1 cut(s) 1333
CfoI GCGC 4 cut(s) 152, 327, 749, 1805
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 2792
CseI GACGC 3 cut(s) 131, 276, 2346
Csp6I GTAC 9 cut(s) 627, 813, 1076, 1329, 1476, 1903, 2563, 2576, 2656
CviQI GTAC 9 cut(s) 627, 813, 1076, 1329, 1476, 1903, 2563, 2576, 2656
DdeI CTNAG 7 cut(s) 153, 282, 698, 809, 1367, 2186, 2418
DpnI GATC 6 cut(s) 331, 344, 729, 1065, 1485, 1617
DpnII GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
DrdI GACNNNNNNGTC 2 cut(s) 53, 286
DseDI GACNNNNNNGTC 2 cut(s) 53, 286
EaeI YGGCCR 1 cut(s) 848
Eam1104I CTCTTC 2 cut(s) 22, 212
EarI CTCTTC 2 cut(s) 22, 212
Eco130I CCWWGG 3 cut(s) 874, 1028, 2409
Eco31I GGTCTC 1 cut(s) 1452
Eco32I GATATC 3 cut(s) 1360, 1507, 1786
Eco47III AGCGCT 1 cut(s) 748
Eco72I CACGTG 1 cut(s) 994
EcoRI GAATTC 1 cut(s) 1837
EcoRII CCWGG 4 cut(s) 863, 1957, 2788, 2831
EcoRV GATATC 3 cut(s) 1360, 1507, 1786
EcoT14I CCWWGG 3 cut(s) 874, 1028, 2409
EcoT22I ATGCAT 1 cut(s) 1277
ErhI CCWWGG 3 cut(s) 874, 1028, 2409
Esp3I CGTCTC 3 cut(s) 172, 294, 1649
FalI AAGNNNNNCTT 2 cut(s) 2362, 2394
FaqI GGGAC 1 cut(s) 586
FauNDI CATATG 2 cut(s) 2587, 2682
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 6 cut(s) 57, 278, 899, 1135, 2010, 2423
Fnu4HI GCNGC 9 cut(s) 74, 1343, 2052, 2444, 2558, 2604, 2607, 2703, 2736
FokI GGATG 6 cut(s) 563, 767, 855, 1180, 1456, 1975
Fsp4HI GCNGC 9 cut(s) 74, 1343, 2052, 2444, 2558, 2604, 2607, 2703, 2736
FspBI CTAG 5 cut(s) 435, 459, 486, 504, 1587
GlaI GCGC 4 cut(s) 151, 326, 748, 1804
GluI GCNGC 9 cut(s) 74, 1343, 2052, 2444, 2558, 2604, 2607, 2703, 2736
GsaI CCCAGC 2 cut(s) 1167, 2556
GsuI CTGGAG 1 cut(s) 2665
HaeII RGCGCY 1 cut(s) 750
HaeIII GGCC 2 cut(s) 850, 2794
HapII CCGG 3 cut(s) 77, 175, 1473
HgaI GACGC 3 cut(s) 131, 276, 2346
HhaI GCGC 4 cut(s) 152, 327, 749, 1805
Hin1I GRCGYC 3 cut(s) 54, 123, 287
Hin6I GCGC 4 cut(s) 150, 325, 747, 1803
HinP1I GCGC 4 cut(s) 150, 325, 747, 1803
HincII GTYRAC 3 cut(s) 58, 279, 766
HindII GTYRAC 3 cut(s) 58, 279, 766
HindIII AAGCTT 1 cut(s) 200
HpaII CCGG 3 cut(s) 77, 175, 1473
HphI GGTGA 2 cut(s) 1721, 2092
Hpy166II GTNNAC 7 cut(s) 58, 279, 766, 900, 1136, 2011, 2424
Hpy188III TCNNGA 8 cut(s) 155, 563, 1081, 1108, 1334, 1451, 2144, 2775
Hpy8I GTNNAC 7 cut(s) 58, 279, 766, 900, 1136, 2011, 2424
Hpy99I CGWCG 3 cut(s) 53, 56, 59
HpyAV CCTTC 4 cut(s) 436, 1539, 1604, 1926
HpyCH4III ACNGT 4 cut(s) 1667, 1907, 2639, 2670
HpyCH4IV ACGT 2 cut(s) 54, 993
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 476, 746, 2024, 2354, 2519, 2566, 2612
HpyF3I CTNAG 7 cut(s) 153, 282, 698, 809, 1367, 2186, 2418
HpySE526I ACGT 2 cut(s) 54, 993
Hsp92I GRCGYC 3 cut(s) 54, 123, 287
HspAI GCGC 4 cut(s) 150, 325, 747, 1803
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
Lsp1109I GCAGC 8 cut(s) 1354, 2038, 2455, 2569, 2590, 2593, 2689, 2747
LweI GCATC 8 cut(s) 60, 877, 1084, 1216, 1264, 1706, 2679, 2780
MaeI CTAG 5 cut(s) 435, 459, 486, 504, 1587
MaeII ACGT 2 cut(s) 54, 993
MalI GATC 6 cut(s) 331, 344, 729, 1065, 1485, 1617
MboI GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
MboII GAAGA 8 cut(s) 39, 131, 229, 866, 1645, 1941, 1960, 2801
MfeI CAATTG 1 cut(s) 1121
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 850
MluNI TGGCCA 1 cut(s) 850
MlyI GAGTC 2 cut(s) 1759, 2725
MmeI TCCRAC 1 cut(s) 1372
Mox20I TGGCCA 1 cut(s) 850
Mph1103I ATGCAT 1 cut(s) 1277
MreI CGCCGGCG 1 cut(s) 76
MroNI GCCGGC 1 cut(s) 76
MroXI GAANNNNTTC 2 cut(s) 612, 1941
MscI TGGCCA 1 cut(s) 850
MslI CAYNNNNRTG 2 cut(s) 68, 873
Msp20I TGGCCA 1 cut(s) 850
MspA1I CMGCKG 1 cut(s) 2051
MspI CCGG 3 cut(s) 77, 175, 1473
MspR9I CCNGG 4 cut(s) 865, 1959, 2790, 2833
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 3 cut(s) 1723, 1898, 2704
MvaI CCWGG 4 cut(s) 865, 1959, 2790, 2833
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 8 cut(s) 131, 476, 746, 2024, 2354, 2519, 2566, 2612
NaeI GCCGGC 1 cut(s) 78
NcoI CCATGG 1 cut(s) 874
NdeI CATATG 2 cut(s) 2587, 2682
NdeII GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
NgoMIV GCCGGC 1 cut(s) 76
NheI GCTAGC 1 cut(s) 503
NlaIV GGNNCC 4 cut(s) 1580, 2164, 2415, 2622
NmuCI GTSAC 6 cut(s) 394, 1048, 1709, 2358, 2639, 2670
NsiI ATGCAT 1 cut(s) 1277
NspI RCATGY 3 cut(s) 1388, 2138, 2456
PagI TCATGA 1 cut(s) 1333
PciI ACATGT 1 cut(s) 2452
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 284
PctI GAATGC 3 cut(s) 1723, 1898, 2704
PdiI GCCGGC 1 cut(s) 78
PdmI GAANNNNTTC 2 cut(s) 612, 1941
PfeI GAWTC 9 cut(s) 230, 258, 917, 1415, 1492, 2085, 2275, 2778, 2806
PflFI GACNNNGTC 1 cut(s) 53
PkrI GCNGC 9 cut(s) 75, 1344, 2053, 2445, 2559, 2605, 2608, 2704, 2737
PleI GAGTC 2 cut(s) 1758, 2724
PmaCI CACGTG 1 cut(s) 994
PmlI CACGTG 1 cut(s) 994
PpsI GAGTC 2 cut(s) 1758, 2724
Ppu21I YACGTR 1 cut(s) 994
PscI ACATGT 1 cut(s) 2452
PshBI ATTAAT 2 cut(s) 611, 2490
Psp6I CCWGG 4 cut(s) 863, 1957, 2788, 2831
PspCI CACGTG 1 cut(s) 994
PspFI CCCAGC 2 cut(s) 1163, 2552
PspGI CCWGG 4 cut(s) 863, 1957, 2788, 2831
PspN4I GGNNCC 4 cut(s) 1580, 2164, 2415, 2622
PspPI GGNCC 1 cut(s) 2792
PstI CTGCAG 5 cut(s) 481, 2445, 2500, 2548, 2737
PstNI CAGNNNCTG 2 cut(s) 2552, 2643
PsyI GACNNNGTC 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 2051
RsaI GTAC 9 cut(s) 628, 814, 1077, 1330, 1477, 1904, 2564, 2577, 2657
RsaNI GTAC 9 cut(s) 627, 813, 1076, 1329, 1476, 1903, 2563, 2576, 2656
RseI CAYNNNNRTG 2 cut(s) 68, 873
SalI GTCGAC 2 cut(s) 56, 277
SatI GCNGC 9 cut(s) 74, 1343, 2052, 2444, 2558, 2604, 2607, 2703, 2736
Sau3AI GATC 6 cut(s) 329, 342, 727, 1063, 1483, 1615
Sau96I GGNCC 1 cut(s) 2792
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 2 cut(s) 1759, 2725
ScrFI CCNGG 4 cut(s) 865, 1959, 2790, 2833
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 8 cut(s) 60, 877, 1084, 1216, 1264, 1706, 2679, 2780
SfcI CTRYAG 7 cut(s) 477, 816, 1704, 2441, 2496, 2544, 2733
SgrAI CRCCGGYG 1 cut(s) 76
SmiMI CAYNNNNRTG 2 cut(s) 68, 873
SmlI CTYRAG 1 cut(s) 465
SmoI CTYRAG 1 cut(s) 465
SsiI CCGC 5 cut(s) 74, 80, 148, 1582, 2507
SspMI CTAG 5 cut(s) 435, 459, 486, 504, 1587
StyD4I CCNGG 4 cut(s) 863, 1957, 2788, 2831
StyI CCWWGG 3 cut(s) 874, 1028, 2409
TaaI ACNGT 4 cut(s) 1667, 1907, 2639, 2670
TaiI ACGT 2 cut(s) 57, 996
TatI WGTACW 3 cut(s) 812, 1075, 2655
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 9 cut(s) 230, 258, 917, 1415, 1492, 2085, 2275, 2778, 2806
TseFI GTSAC 6 cut(s) 394, 1048, 1709, 2358, 2639, 2670
TseI GCWGC 8 cut(s) 1342, 2051, 2443, 2557, 2603, 2606, 2702, 2735
Tsp45I GTSAC 6 cut(s) 394, 1048, 1709, 2358, 2639, 2670
TspGWI ACGGA 1 cut(s) 1259
Tth111I GACNNNGTC 1 cut(s) 53
VspI ATTAAT 2 cut(s) 611, 2490
XapI RAATTY 3 cut(s) 1837, 2171, 2844
XceI RCATGY 3 cut(s) 1388, 2138, 2456
XmiI GTMKAC 6 cut(s) 57, 278, 899, 1135, 2010, 2423
XmnI GAANNNNTTC 2 cut(s) 612, 1941
XspI CTAG 5 cut(s) 435, 459, 486, 504, 1587
ZraI GACGTC 1 cut(s) 55
ZrmI AGTACT 1 cut(s) 814
Zsp2I ATGCAT 1 cut(s) 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.