AT5G51140

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009164
Physical Location & Seq
Reverse (-)
20783931 .. 20786843
2913 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51140.1

Sequence Viewer

Length: 1188 bp
ATGAAACGAAAGCAGCAAGAAGACGACAACGACGACGGCGTAGAGAAAGCCGTCTCTCCCGTCCTCGCCACTACCTCAAACACCATGAGTACTGATCTACTTTTACAGTCATCTTCTAAACTATCACAGAAACAGGACTACATCTTCCATGGAGGAAGACGCCATGTGAGACCTTACTACTTCGAGTTCATTTCTCATGTGAACAAACGATGGACTGGGAAAACAATTGTGGATTTGTTTGCTGATGAATTTAAAGGACGGCCTCGTGATTATTATGTTGGTGCAGTCAAATCTGGAAGAATCAAAGTTGATGGAGAAATTGTACCGGTTTCATACATTGTGAAATCATCCCAAAAGATTACTCACTTCCTCCACAGGCATGAACCACCGGTGATGATTGATGATGTTGTGATCCTTCATCAAGAACCTGACGTTGTTACTGTCTGCAAACCAGCTTCTGTTCCTGTGCATCCATGTGGTCAATACCGTAAGAACACGATTGTTGGCATCCTTGATGCTGAGCATGACCTAGGAACTCTATTTCCTATTCACAGATTAGACCGTTTGGTTTCTGGATTGCTTATCATAGCAAGAACTGCGGCAAAAGCTGATTTTTTCAGGCAACAGATTGAGGGTGGAATGGTGAAGAAACGTTATATTGCAAAGGTTATTGGCGTGTTCCCAGAAGATGAGATGATAGTCGACGCCAACATAAATTATAATGGCAGCGAAGGAAGAAGTACAGCAGAGGACGCCAATTCAAGTGGTGACGATAAGAAGGTGAAGGGGAAGCCTGCATGCACGAAGTTCACTAGAATCGATACAAATGGGACTCATAGCCTCGTCTCATGTGAACCAGTGACAGGGCGAACCCATCAAATACGAGTGCATCTACAATATACAGGGCATCCCATAGCGAATGACCCTCTTTATCTCAATCAACACATAGATAATCTTGAGACCTACATTGCAAAAAGAATCGATGCGGGTGAGAGAAAGATTGTCTCCCCAGATGACTACGTGTATTCAAGCGAAGATTTCAGCATCGATCCAATGTGTACGAACTGCCCAAAGTTGATCCCACAAGGATATGAGGAGCATGACGAGGCTCTCTGGTTACATTGCGTTCAATACTGTGGAACTGGATGGGAATACGAATGTCCTTATCCATCTTGGGCATCCCTTTAG

Protein Analysis

395

Amino Acids

44.62

Weight (kDa)

6.28

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 144 - 299 8.4e-29 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AccI GTMKAC 1 cut(s) 702
AciI CCGC 2 cut(s) 599, 986
AclI AACGTT 1 cut(s) 652
AclWI GGATC 3 cut(s) 406, 1043, 1072
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 3 cut(s) 160, 705, 753
AfaI GTAC 4 cut(s) 91, 324, 742, 1060
AfiI CCNNNNNNNGG 1 cut(s) 863
AflIII ACRYGT 1 cut(s) 1020
AgeI ACCGGT 2 cut(s) 325, 388
AgsI TTSAA 3 cut(s) 762, 1029, 1130
AloI GAACNNNNNNTCC 2 cut(s) 526, 558
AluBI AGCT 2 cut(s) 455, 608
AluI AGCT 2 cut(s) 455, 608
Alw26I GTCTC 5 cut(s) 58, 163, 850, 953, 1009
AlwI GGATC 3 cut(s) 406, 1043, 1072
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 13, 726
ApoI RAATTY 1 cut(s) 248
AsiGI ACCGGT 2 cut(s) 325, 388
AspA2I CCTAGG 1 cut(s) 529
AsuHPI GGTGA 5 cut(s) 403, 655, 779, 793, 1001
AvrII CCTAGG 1 cut(s) 529
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 2 cut(s) 27, 163
BbvI GCAGC 2 cut(s) 25, 738
BccI CCATC 5 cut(s) 204, 305, 882, 1140, 1177
BceAI ACGGC 3 cut(s) 35, 52, 275
BcoDI GTCTC 5 cut(s) 58, 163, 850, 953, 1009
BfaI CTAG 2 cut(s) 530, 813
BisI GCNGC 3 cut(s) 14, 600, 727
BlnI CCTAGG 1 cut(s) 529
BlpI GCTNAGC 1 cut(s) 519
BlsI GCNGC 3 cut(s) 15, 601, 728
BmcAI AGTACT 1 cut(s) 91
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 7 cut(s) 478, 505, 516, 898, 916, 973, 1053
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 2 cut(s) 27, 163
Bpu1102I GCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 977
Bsa29I ATCGAT 3 cut(s) 819, 981, 1047
BsaAI YACGTR 1 cut(s) 1021
BsaHI GRCGYC 3 cut(s) 160, 705, 753
BsaI GGTCTC 2 cut(s) 163, 953
BsaJI CCNNGG 2 cut(s) 148, 529
BsaWI WCCGGW 2 cut(s) 325, 388
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 863
Bse118I RCCGGY 2 cut(s) 325, 388
Bse1I ACTGG 3 cut(s) 220, 857, 1147
Bse3DI GCAATG 2 cut(s) 966, 1120
BseCI ATCGAT 3 cut(s) 819, 981, 1047
BseDI CCNNGG 2 cut(s) 148, 529
BseGI GGATG 6 cut(s) 347, 469, 507, 907, 1151, 1178
BseLI CCNNNNNNNGG 1 cut(s) 863
BseMI GCAATG 2 cut(s) 966, 1120
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 3 cut(s) 220, 857, 1147
BseRI GAGGAG 1 cut(s) 1109
BseXI GCAGC 2 cut(s) 25, 738
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 1 cut(s) 262
BshTI ACCGGT 2 cut(s) 325, 388
BshVI ATCGAT 3 cut(s) 819, 981, 1047
BsiSI CCGG 2 cut(s) 326, 389
BslFI GGGAC 1 cut(s) 844
BslI CCNNNNNNNGG 1 cut(s) 863
BsmAI GTCTC 5 cut(s) 58, 163, 850, 953, 1009
BsmBI CGTCTC 2 cut(s) 58, 850
BsmFI GGGAC 1 cut(s) 844
BsnI GGCC 1 cut(s) 262
Bso31I GGTCTC 2 cut(s) 163, 953
Bsp143I GATC 4 cut(s) 94, 411, 1048, 1077
Bsp1720I GCTNAGC 1 cut(s) 519
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 2 cut(s) 599, 986
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 511
BspDI ATCGAT 3 cut(s) 819, 981, 1047
BspPI GGATC 3 cut(s) 406, 1043, 1072
BspTNI GGTCTC 2 cut(s) 163, 953
BsrDI GCAATG 2 cut(s) 966, 1120
BsrFI RCCGGY 2 cut(s) 325, 388
BsrI ACTGG 3 cut(s) 220, 857, 1147
BssAI RCCGGY 2 cut(s) 325, 388
BssECI CCNNGG 2 cut(s) 148, 529
BssMI GATC 4 cut(s) 94, 411, 1048, 1077
BssNI GRCGYC 3 cut(s) 160, 705, 753
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 2 cut(s) 148, 529
Bst2BI CACGAG 1 cut(s) 264
Bst4CI ACNGT 5 cut(s) 108, 442, 488, 563, 1136
BstACI GRCGYC 3 cut(s) 160, 705, 753
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 1021
BstC8I GCNNGC 2 cut(s) 795, 799
BstDEI CTNAG 1 cut(s) 519
BstDSI CCRYGG 1 cut(s) 148
BstF5I GGATG 6 cut(s) 347, 469, 507, 907, 1151, 1178
BstKTI GATC 4 cut(s) 97, 414, 1051, 1080
BstMAI GTCTC 5 cut(s) 58, 163, 850, 953, 1009
BstMBI GATC 4 cut(s) 94, 411, 1048, 1077
BstMWI GCNNNNNNNGC 3 cut(s) 596, 605, 752
BstNSI RCATGY 1 cut(s) 801
BstV1I GCAGC 2 cut(s) 25, 738
BstV2I GAAGAC 2 cut(s) 27, 163
Bsu15I ATCGAT 3 cut(s) 819, 981, 1047
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 3 cut(s) 819, 981, 1047
BtgI CCRYGG 1 cut(s) 148
BtsCI GGATG 6 cut(s) 347, 469, 507, 907, 1151, 1178
BtsIMutI CAGTG 1 cut(s) 864
Cac8I GCNNGC 2 cut(s) 795, 799
CaiI CAGNNNCTG 1 cut(s) 458
Cfr10I RCCGGY 2 cut(s) 325, 388
ClaI ATCGAT 3 cut(s) 819, 981, 1047
CseI GACGC 3 cut(s) 168, 713, 761
Csp6I GTAC 4 cut(s) 90, 323, 741, 1059
CspAI ACCGGT 2 cut(s) 325, 388
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
CviJI RGCY 7 cut(s) 50, 262, 455, 608, 793, 840, 1109
CviKI_1 RGCY 7 cut(s) 50, 262, 455, 608, 793, 840, 1109
CviQI GTAC 4 cut(s) 90, 323, 741, 1059
DdeI CTNAG 1 cut(s) 519
DpnI GATC 4 cut(s) 96, 413, 1050, 1079
DpnII GATC 4 cut(s) 94, 411, 1048, 1077
DraI TTTAAA 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 148, 529
Eco31I GGTCTC 2 cut(s) 163, 953
EcoT14I CCWWGG 2 cut(s) 148, 529
ErhI CCWWGG 2 cut(s) 148, 529
Esp3I CGTCTC 2 cut(s) 58, 850
FaqI GGGAC 1 cut(s) 844
FauI CCCGC 1 cut(s) 979
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 3 cut(s) 14, 600, 727
FokI GGATG 6 cut(s) 334, 456, 494, 894, 1158, 1165
Fsp4HI GCNGC 3 cut(s) 14, 600, 727
FspBI CTAG 2 cut(s) 530, 813
GluI GCNGC 3 cut(s) 14, 600, 727
HaeIII GGCC 1 cut(s) 262
HapII CCGG 2 cut(s) 326, 389
HgaI GACGC 3 cut(s) 168, 713, 761
Hin1I GRCGYC 3 cut(s) 160, 705, 753
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 4 cut(s) 300, 816, 832, 978
HpaII CCGG 2 cut(s) 326, 389
HphI GGTGA 5 cut(s) 403, 655, 779, 793, 1001
Hpy166II GTNNAC 5 cut(s) 202, 703, 810, 854, 1059
Hpy188III TCNNGA 5 cut(s) 266, 294, 422, 573, 956
Hpy8I GTNNAC 5 cut(s) 202, 703, 810, 854, 1059
Hpy99I CGWCG 3 cut(s) 35, 38, 707
HpyAV CCTTC 4 cut(s) 425, 725, 772, 778
HpyCH4III ACNGT 5 cut(s) 108, 442, 488, 563, 1136
HpyCH4IV ACGT 3 cut(s) 432, 652, 1020
HpyCH4V TGCA 8 cut(s) 284, 447, 469, 662, 797, 801, 889, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 605, 752
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 3 cut(s) 432, 652, 1020
Hsp92I GRCGYC 3 cut(s) 160, 705, 753
Kzo9I GATC 4 cut(s) 94, 411, 1048, 1077
LmnI GCTCC 1 cut(s) 1096
Lsp1109I GCAGC 2 cut(s) 25, 738
LweI GCATC 7 cut(s) 478, 505, 516, 898, 916, 973, 1053
MaeI CTAG 2 cut(s) 530, 813
MaeII ACGT 3 cut(s) 432, 652, 1020
MaeIII GTNAC 4 cut(s) 436, 767, 859, 1116
MalI GATC 4 cut(s) 96, 413, 1050, 1079
MboI GATC 4 cut(s) 94, 411, 1048, 1077
MboII GAAGA 9 cut(s) 32, 105, 136, 168, 309, 658, 698, 747, 1046
MfeI CAATTG 1 cut(s) 225
MluCI AATT 5 cut(s) 225, 248, 318, 715, 757
MlyI GAGTC 1 cut(s) 826
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 2 cut(s) 378, 474
MspI CCGG 2 cut(s) 326, 389
MunI CAATTG 1 cut(s) 225
MwoI GCNNNNNNNGC 3 cut(s) 596, 605, 752
NcoI CCATGG 1 cut(s) 148
NdeII GATC 4 cut(s) 94, 411, 1048, 1077
NmuCI GTSAC 2 cut(s) 767, 859
NspI RCATGY 1 cut(s) 801
PaeI GCATGC 1 cut(s) 801
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 36
PfeI GAWTC 3 cut(s) 300, 816, 978
PinAI ACCGGT 2 cut(s) 325, 388
PkrI GCNGC 3 cut(s) 15, 601, 728
PleI GAGTC 1 cut(s) 826
PpsI GAGTC 1 cut(s) 826
Ppu21I YACGTR 1 cut(s) 1021
PsiI TTATAA 1 cut(s) 720
Psp1406I AACGTT 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 458
RsaI GTAC 4 cut(s) 91, 324, 742, 1060
RsaNI GTAC 4 cut(s) 90, 323, 741, 1059
RseI CAYNNNNRTG 2 cut(s) 378, 474
SalI GTCGAC 1 cut(s) 701
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 3 cut(s) 14, 600, 727
Sau3AI GATC 4 cut(s) 94, 411, 1048, 1077
ScaI AGTACT 1 cut(s) 91
SchI GAGTC 1 cut(s) 826
SfaNI GCATC 7 cut(s) 478, 505, 516, 898, 916, 973, 1053
SgrAI CRCCGGYG 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 378, 474
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SphI GCATGC 1 cut(s) 801
Sse9I AATT 5 cut(s) 225, 248, 318, 715, 757
SsiI CCGC 2 cut(s) 599, 986
SspMI CTAG 2 cut(s) 530, 813
StyI CCWWGG 2 cut(s) 148, 529
TaaI ACNGT 5 cut(s) 108, 442, 488, 563, 1136
TaiI ACGT 3 cut(s) 435, 655, 1023
TaqI TCGA 5 cut(s) 183, 702, 819, 981, 1047
TasI AATT 5 cut(s) 225, 248, 318, 715, 757
TatI WGTACW 2 cut(s) 89, 740
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 3 cut(s) 300, 816, 978
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 1 cut(s) 864
TseFI GTSAC 2 cut(s) 767, 859
TseI GCWGC 2 cut(s) 13, 726
Tsp45I GTSAC 2 cut(s) 767, 859
TspDTI ATGAA 6 cut(s) 17, 178, 261, 321, 396, 407
TspRI CASTG 1 cut(s) 864
XapI RAATTY 1 cut(s) 248
XceI RCATGY 1 cut(s) 801
XmaJI CCTAGG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 702
XspI CTAG 2 cut(s) 530, 813
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.