Rw7G008850

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009164
Physical Location & Seq
Reverse (-)
8194881 .. 8206391
11511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008850.1

Sequence Viewer

Length: 1173 bp
ATGCCAACGACGTCGTTTGCTTCTAGGGCTCTGCAAATTTGCAAAAATTTGATCCCAAATCAGAATAAAAGAGGAAAAATGAAGAGGAAGATAGAAGAGACGGACATAGAGATCGTTTGGCAATCTCCCAAATTTGTTCAATTAGGGAGGCGCCATGTGAGACCCTACTACTTTGAGTTCATTGCTCATGTTAAGAACCGGTGGGCGGGGAAGACCATCGTTGACTTGTTCACTGACGAGTTCAAAGGCCGGAATCGTGACTACTATATTAGTGCGGTTAAATGTGGGAGGATACAAGTTGATGGGCAACTTGTGCCGGTTTCCTTCATAGTTAAACCGTCGCAAAAGATAAGCCACTTCCTGCACAGGCATGAACCGCCCGTGATGACTTGGGATGTGAAAATTCTTGAAACAGAACCCGATGTGGTCACCATTTGTAAACCTGCATCTGTTCCAGTGCATCCATGTGGTCAGTATCGTAAGAACACTGTTGTTGGCATCCTACAGGCAGAGCATGGCTTGGCACCTCTATTTCCGATTCATCGATTAGATCGTCTGGTCTCAGGACTCCTTATCTTGGCTAGAAGCGCAGCACAAGCTGACATTTTCAGGCAACAGATTGAGGGAGGGATGGTTCATAAACAGTACATTGCAAGAGTCATTGGAGAGTTTCCTGAGGAGGAGCAAGTTGTAGATGCTAACATAAATCATAATGCTCGAGAAGGAAGGAGCTCTACCGAGGGGAAGACTGCTTGTACAAAGTTTACTAGAATCAGTACGAATGGAATTCAGAGCATTGTCTTGTGTGAACCAGTCACTGGCCGCACTCATCAGATACGCGTCCATTTGCAACATGCAGGCCATCCAATAGCGAATGACATGCTTTACCTGTCCAAACATGTAAGTGATCGTTCAGCTGAAGGAATGGGTGCTGATAGAGCTGCTGCTAATCCAGACCACTCTCCAGCTTCTGGTGTTGGCGAAAATAAATCCGCTTCGCATGAAGATTCTGGTGAGGACTTCAGCATTGATCCAATGTGTACAAATTGTCCAAGTTTAGCTCCAAAAGGATACGATTATCATGAAGAGGCCTTGTGGCTACACTGTTTTAGATATTCTGGACCTGGATGGGTTTATGAGTGCCCACATCCGGATTGGGCATCACTTAGCTAG

Protein Analysis

390

Amino Acids

43.84

Weight (kDa)

8.27

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 141 - 285 4.1e-29 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 451
AccB1I GGYRCC 2 cut(s) 150, 523
AccB7I CCANNNNNTGG 2 cut(s) 818, 971
AccII CGCG 1 cut(s) 840
AccIII TCCGGA 1 cut(s) 1150
AciI CCGC 5 cut(s) 206, 275, 377, 823, 993
AclWI GGATC 2 cut(s) 46, 1025
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 5 cut(s) 36, 46, 131, 402, 786
AcuI CTGAAG 2 cut(s) 939, 1006
AcyI GRCGYC 2 cut(s) 11, 151
AfaI GTAC 4 cut(s) 647, 757, 778, 1042
AfiI CCNNNNNNNGG 5 cut(s) 205, 577, 818, 971, 1150
AflIII ACRYGT 2 cut(s) 838, 898
AgeI ACCGGT 1 cut(s) 198
AgsI TTSAA 3 cut(s) 140, 244, 410
AjnI CCWGG 1 cut(s) 1123
AloI GAACNNNNNNTCC 2 cut(s) 618, 650
AluBI AGCT 7 cut(s) 599, 732, 917, 941, 968, 1061, 1170
AluI AGCT 7 cut(s) 599, 732, 917, 941, 968, 1061, 1170
Alw21I GWGCWC 1 cut(s) 734
Alw26I GTCTC 3 cut(s) 92, 154, 565
AlwI GGATC 2 cut(s) 46, 1025
AlwNI CAGNNNCTG 2 cut(s) 818, 971
Ama87I CYCGRG 1 cut(s) 717
Aor13HI TCCGGA 1 cut(s) 1150
AoxI GGCC 4 cut(s) 247, 820, 859, 1089
ApeKI GCWGC 3 cut(s) 590, 941, 944
ApoI RAATTY 5 cut(s) 36, 46, 131, 402, 786
AsiGI ACCGGT 1 cut(s) 198
AspLEI GCGC 2 cut(s) 153, 590
AspS9I GGNCC 1 cut(s) 1121
AsuHPI GGTGA 2 cut(s) 421, 1025
AvaI CYCGRG 1 cut(s) 717
AvaII GGWCC 1 cut(s) 1121
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 1 cut(s) 1145
BanI GGYRCC 2 cut(s) 150, 523
BanII GRGCYC 2 cut(s) 31, 734
BarI GAAGNNNNNNTAC 2 cut(s) 718, 750
BbsI GAAGAC 2 cut(s) 218, 752
Bbv12I GWGCWC 1 cut(s) 734
BbvI GCAGC 3 cut(s) 602, 928, 931
BccI CCATC 5 cut(s) 224, 296, 625, 870, 1122
BcgI CGANNNNNNTGC 2 cut(s) 862, 896
BciT130I CCWGG 1 cut(s) 1125
BciVI GTATCC 2 cut(s) 285, 1064
BcoDI GTCTC 3 cut(s) 92, 154, 565
BfaI CTAG 4 cut(s) 24, 582, 768, 1171
BfmI CTRYAG 1 cut(s) 503
BfoI RGCGCY 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 2 cut(s) 285, 1064
BisI GCNGC 4 cut(s) 591, 823, 942, 945
BlsI GCNGC 4 cut(s) 592, 824, 943, 946
Bme1390I CCNGG 1 cut(s) 1125
Bme18I GGWCC 1 cut(s) 1121
BmeT110I CYCGRG 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 1121
BmiI GGNNCC 2 cut(s) 152, 525
BmrFI CCNGG 1 cut(s) 1125
BmsI GCATC 5 cut(s) 455, 469, 507, 685, 1169
BpiI GAAGAC 2 cut(s) 218, 752
BpmI CTGGAG 1 cut(s) 948
Bsa29I ATCGAT 1 cut(s) 544
BsaHI GRCGYC 2 cut(s) 11, 151
BsaI GGTCTC 2 cut(s) 154, 565
BsaJI CCNNGG 1 cut(s) 738
BsaWI WCCGGW 2 cut(s) 198, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 577, 818, 971, 1150
Bse118I RCCGGY 2 cut(s) 198, 316
Bse1I ACTGG 3 cut(s) 455, 812, 823
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 2 cut(s) 180, 648
BseAI TCCGGA 1 cut(s) 1150
BseBI CCWGG 1 cut(s) 1125
BseCI ATCGAT 1 cut(s) 544
BseDI CCNNGG 1 cut(s) 738
BseGI GGATG 7 cut(s) 400, 460, 498, 636, 862, 1133, 1147
BseLI CCNNNNNNNGG 5 cut(s) 205, 577, 818, 971, 1150
BseMI GCAATG 2 cut(s) 180, 648
BseMII CTCAG 2 cut(s) 576, 666
BseNI ACTGG 3 cut(s) 455, 812, 823
BseRI GAGGAG 2 cut(s) 692, 695
BseSI GKGCMC 1 cut(s) 1145
BseXI GCAGC 3 cut(s) 602, 928, 931
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 4 cut(s) 249, 822, 861, 1091
BshNI GGYRCC 2 cut(s) 150, 523
BshTI ACCGGT 1 cut(s) 198
BshVI ATCGAT 1 cut(s) 544
BsiHKAI GWGCWC 1 cut(s) 734
BsiHKCI CYCGRG 1 cut(s) 717
BsiSI CCGG 4 cut(s) 199, 250, 317, 1151
BslI CCNNNNNNNGG 5 cut(s) 205, 577, 818, 971, 1150
BsmAI GTCTC 3 cut(s) 92, 154, 565
BsmBI CGTCTC 1 cut(s) 92
BsnI GGCC 4 cut(s) 249, 822, 861, 1091
Bso31I GGTCTC 2 cut(s) 154, 565
BsoBI CYCGRG 1 cut(s) 717
Bsp1286I GDGCHC 3 cut(s) 31, 734, 1145
Bsp13I TCCGGA 1 cut(s) 1150
Bsp1407I TGTACA 2 cut(s) 755, 1040
Bsp143I GATC 5 cut(s) 51, 111, 550, 907, 1030
BspACI CCGC 5 cut(s) 206, 275, 377, 823, 993
BspANI GGCC 4 cut(s) 249, 822, 861, 1091
BspCNI CTCAG 2 cut(s) 575, 667
BspDI ATCGAT 1 cut(s) 544
BspEI TCCGGA 1 cut(s) 1150
BspFNI CGCG 1 cut(s) 840
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 2 cut(s) 152, 525
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 2 cut(s) 46, 1025
BspT107I GGYRCC 2 cut(s) 150, 523
BspTNI GGTCTC 2 cut(s) 154, 565
BsrDI GCAATG 2 cut(s) 180, 648
BsrFI RCCGGY 2 cut(s) 198, 316
BsrGI TGTACA 2 cut(s) 755, 1040
BsrI ACTGG 3 cut(s) 455, 812, 823
BssAI RCCGGY 2 cut(s) 198, 316
BssECI CCNNGG 1 cut(s) 738
BssMI GATC 5 cut(s) 51, 111, 550, 907, 1030
BssNI GRCGYC 2 cut(s) 11, 151
Bst2UI CCWGG 1 cut(s) 1125
Bst4CI ACNGT 4 cut(s) 339, 490, 645, 1106
Bst6I CTCTTC 3 cut(s) 77, 90, 1080
BstACI GRCGYC 2 cut(s) 11, 151
BstAPI GCANNNNNTGC 1 cut(s) 313
BstAUI TGTACA 2 cut(s) 755, 1040
BstC8I GCNNGC 1 cut(s) 859
BstDEI CTNAG 3 cut(s) 562, 675, 1166
BstEII GGTNACC 1 cut(s) 427
BstF5I GGATG 7 cut(s) 400, 460, 498, 636, 862, 1133, 1147
BstFNI CGCG 1 cut(s) 840
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 2 cut(s) 153, 590
BstKTI GATC 5 cut(s) 54, 114, 553, 910, 1033
BstMAI GTCTC 3 cut(s) 92, 154, 565
BstMBI GATC 5 cut(s) 51, 111, 550, 907, 1030
BstMWI GCNNNNNNNGC 6 cut(s) 26, 313, 376, 587, 596, 938
BstNI CCWGG 1 cut(s) 1125
BstNSI RCATGY 3 cut(s) 857, 883, 902
BstPI GGTNACC 1 cut(s) 427
BstSCI CCNGG 1 cut(s) 1123
BstSFI CTRYAG 1 cut(s) 503
BstSLI GKGCMC 1 cut(s) 1145
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 3 cut(s) 602, 928, 931
BstV2I GAAGAC 2 cut(s) 218, 752
Bsu15I ATCGAT 1 cut(s) 544
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 2 cut(s) 285, 1064
BsuRI GGCC 4 cut(s) 249, 822, 861, 1091
BsuTUI ATCGAT 1 cut(s) 544
BtsCI GGATG 7 cut(s) 400, 460, 498, 636, 862, 1133, 1147
BtsIMutI CAGTG 5 cut(s) 231, 462, 486, 816, 1102
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 1 cut(s) 859
CaiI CAGNNNCTG 2 cut(s) 818, 971
CciI TCATGA 1 cut(s) 1081
CfoI GCGC 2 cut(s) 153, 590
Cfr10I RCCGGY 2 cut(s) 198, 316
Cfr13I GGNCC 1 cut(s) 1121
ClaI ATCGAT 1 cut(s) 544
CseI GACGC 1 cut(s) 829
Csp6I GTAC 4 cut(s) 646, 756, 777, 1041
CspAI ACCGGT 1 cut(s) 198
CviQI GTAC 4 cut(s) 646, 756, 777, 1041
DdeI CTNAG 3 cut(s) 562, 675, 1166
DinI GGCGCC 1 cut(s) 152
DpnI GATC 5 cut(s) 53, 113, 552, 909, 1032
DpnII GATC 5 cut(s) 51, 111, 550, 907, 1030
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 3 cut(s) 77, 90, 1080
EarI CTCTTC 3 cut(s) 77, 90, 1080
Ecl136II GAGCTC 1 cut(s) 732
Eco147I AGGCCT 1 cut(s) 1091
Eco24I GRGCYC 2 cut(s) 31, 734
Eco31I GGTCTC 2 cut(s) 154, 565
Eco47I GGWCC 1 cut(s) 1121
Eco53kI GAGCTC 1 cut(s) 732
Eco57I CTGAAG 2 cut(s) 939, 1006
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 717
Eco91I GGTNACC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 732
EcoO65I GGTNACC 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 786
EcoRII CCWGG 1 cut(s) 1123
EcoT38I GRGCYC 2 cut(s) 31, 734
EgeI GGCGCC 1 cut(s) 152
EheI GGCGCC 1 cut(s) 152
Esp3I CGTCTC 1 cut(s) 92
FauI CCCGC 1 cut(s) 199
Fnu4HI GCNGC 4 cut(s) 591, 823, 942, 945
FokI GGATG 7 cut(s) 407, 447, 485, 643, 849, 1134, 1140
FriOI GRGCYC 2 cut(s) 31, 734
Fsp4HI GCNGC 4 cut(s) 591, 823, 942, 945
FspBI CTAG 4 cut(s) 24, 582, 768, 1171
GlaI GCGC 2 cut(s) 152, 589
GluI GCNGC 4 cut(s) 591, 823, 942, 945
GsuI CTGGAG 1 cut(s) 948
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 4 cut(s) 249, 822, 861, 1091
HapII CCGG 4 cut(s) 199, 250, 317, 1151
HgaI GACGC 1 cut(s) 829
HhaI GCGC 2 cut(s) 153, 590
Hin1I GRCGYC 2 cut(s) 11, 151
Hin6I GCGC 2 cut(s) 151, 588
HinP1I GCGC 2 cut(s) 151, 588
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 6 cut(s) 253, 538, 567, 657, 771, 1007
HpaII CCGG 4 cut(s) 199, 250, 317, 1151
HphI GGTGA 2 cut(s) 421, 1025
Hpy166II GTNNAC 6 cut(s) 223, 231, 440, 765, 809, 1041
Hpy188I TCNGA 4 cut(s) 63, 537, 792, 834
Hpy188III TCNNGA 9 cut(s) 257, 407, 564, 674, 719, 953, 1082, 1119, 1151
Hpy8I GTNNAC 6 cut(s) 223, 231, 440, 765, 809, 1041
Hpy99I CGWCG 3 cut(s) 13, 16, 343
HpyAV CCTTC 4 cut(s) 334, 716, 720, 914
HpyCH4III ACNGT 4 cut(s) 339, 490, 645, 1106
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 8 cut(s) 34, 42, 364, 446, 460, 653, 850, 857
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 313, 376, 587, 596, 938
HpyF3I CTNAG 3 cut(s) 562, 675, 1166
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 2 cut(s) 11, 151
HspAI GCGC 2 cut(s) 151, 588
KasI GGCGCC 1 cut(s) 150
Kpn2I TCCGGA 1 cut(s) 1150
Kzo9I GATC 5 cut(s) 51, 111, 550, 907, 1030
LmnI GCTCC 3 cut(s) 682, 729, 1066
Lsp1109I GCAGC 3 cut(s) 602, 928, 931
LweI GCATC 5 cut(s) 455, 469, 507, 685, 1169
MaeI CTAG 4 cut(s) 24, 582, 768, 1171
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 3 cut(s) 257, 427, 814
MalI GATC 5 cut(s) 53, 113, 552, 909, 1032
MboI GATC 5 cut(s) 51, 111, 550, 907, 1030
MboII GAAGA 7 cut(s) 94, 100, 107, 223, 757, 1016, 1097
MhlI GDGCHC 3 cut(s) 31, 734, 1145
MluCI AATT 7 cut(s) 36, 46, 131, 140, 402, 786, 1045
MluI ACGCGT 1 cut(s) 838
Mly113I GGCGCC 1 cut(s) 151
MlyI GAGTC 2 cut(s) 561, 666
MroI TCCGGA 1 cut(s) 1150
MseI TTAA 3 cut(s) 192, 279, 333
MslI CAYNNNNRTG 3 cut(s) 369, 465, 903
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 4 cut(s) 199, 250, 317, 1151
MspR9I CCNGG 1 cut(s) 1125
MvaI CCWGG 1 cut(s) 1125
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 6 cut(s) 26, 313, 376, 587, 596, 938
NarI GGCGCC 1 cut(s) 151
NdeII GATC 5 cut(s) 51, 111, 550, 907, 1030
NlaIV GGNNCC 2 cut(s) 152, 525
NmuCI GTSAC 3 cut(s) 257, 427, 814
NspI RCATGY 3 cut(s) 857, 883, 902
PaeR7I CTCGAG 1 cut(s) 717
PagI TCATGA 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 1091
PciI ACATGT 1 cut(s) 898
PcsI WCGNNNNNNNCGW 1 cut(s) 550
PfeI GAWTC 4 cut(s) 253, 538, 771, 1007
PflMI CCANNNNNTGG 2 cut(s) 818, 971
PinAI ACCGGT 1 cut(s) 198
PkrI GCNGC 4 cut(s) 592, 824, 943, 946
PleI GAGTC 2 cut(s) 561, 665
PluTI GGCGCC 1 cut(s) 154
PpsI GAGTC 2 cut(s) 561, 665
PscI ACATGT 1 cut(s) 898
Psp124BI GAGCTC 1 cut(s) 734
Psp6I CCWGG 1 cut(s) 1123
PspEI GGTNACC 1 cut(s) 427
PspGI CCWGG 1 cut(s) 1123
PspN4I GGNNCC 2 cut(s) 152, 525
PspPI GGNCC 1 cut(s) 1121
PstNI CAGNNNCTG 2 cut(s) 818, 971
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 4 cut(s) 647, 757, 778, 1042
RsaNI GTAC 4 cut(s) 646, 756, 777, 1041
RseI CAYNNNNRTG 3 cut(s) 369, 465, 903
SacI GAGCTC 1 cut(s) 734
SaqAI TTAA 3 cut(s) 192, 279, 333
SatI GCNGC 4 cut(s) 591, 823, 942, 945
Sau3AI GATC 5 cut(s) 51, 111, 550, 907, 1030
Sau96I GGNCC 1 cut(s) 1121
SchI GAGTC 2 cut(s) 561, 666
ScrFI CCNGG 1 cut(s) 1125
SduI GDGCHC 3 cut(s) 31, 734, 1145
SfaNI GCATC 5 cut(s) 455, 469, 507, 685, 1169
SfcI CTRYAG 1 cut(s) 503
SfoI GGCGCC 1 cut(s) 152
Sfr274I CTCGAG 1 cut(s) 717
SinI GGWCC 1 cut(s) 1121
SlaI CTCGAG 1 cut(s) 717
SmiMI CAYNNNNRTG 3 cut(s) 369, 465, 903
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
Sse9I AATT 7 cut(s) 36, 46, 131, 140, 402, 786, 1045
SseBI AGGCCT 1 cut(s) 1091
SsiI CCGC 5 cut(s) 206, 275, 377, 823, 993
SspDI GGCGCC 1 cut(s) 150
SspMI CTAG 4 cut(s) 24, 582, 768, 1171
SstI GAGCTC 1 cut(s) 734
StuI AGGCCT 1 cut(s) 1091
StyD4I CCNGG 1 cut(s) 1123
TaaI ACNGT 4 cut(s) 339, 490, 645, 1106
TaiI ACGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 544, 718
TasI AATT 7 cut(s) 36, 46, 131, 140, 402, 786, 1045
TatI WGTACW 3 cut(s) 645, 755, 1040
TauI GCSGC 1 cut(s) 825
TfiI GAWTC 4 cut(s) 253, 538, 771, 1007
Tru1I TTAA 3 cut(s) 192, 279, 333
Tru9I TTAA 3 cut(s) 192, 279, 333
TscAI CASTG 5 cut(s) 238, 462, 493, 823, 1109
TseFI GTSAC 3 cut(s) 257, 427, 814
TseI GCWGC 3 cut(s) 590, 941, 944
Tsp45I GTSAC 3 cut(s) 257, 427, 814
TspDTI ATGAA 8 cut(s) 95, 169, 316, 387, 530, 626, 1017, 1098
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 5 cut(s) 238, 462, 493, 823, 1109
Van91I CCANNNNNTGG 2 cut(s) 818, 971
VpaK11BI GGWCC 1 cut(s) 1121
XapI RAATTY 5 cut(s) 36, 46, 131, 402, 786
XceI RCATGY 3 cut(s) 857, 883, 902
XcmI CCANNNNNNNNNTGG 1 cut(s) 1152
XhoI CTCGAG 1 cut(s) 717
XspI CTAG 4 cut(s) 24, 582, 768, 1171
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.