FvH4_5g08961

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009164
Physical Location & Seq
Forward (+)
5175758 .. 5179998
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08961.t3

Sequence Viewer

Length: 1152 bp
ATGAAGAGGAAGATAGAAGAGACGGACATGGAGATCGTCTGGCAGTCGCCGGCGAACCCGCCGGAGCGGAAAGATTACATCTTTCGCAACGGGAGGCGCCATGTGAGACCCTACTACTTTGAGTTCATTGCTCATGTTAAGAATAGGTGGGCGGGGAAGACCATCGTTGACTTGTTCACTGACGAGTTCAAAGGCCGGAATCGTGACTACTATATTAGTGCAGTGAAATGTGGGAGGATACAAGTTGATGGGCAGCTTGTGCCGGTTTCATTCATAGTTAAACCGTCGCAAAAGATAAGCCACTTCCTGCACAGGCATGAACCGCCAGTGATGACTTGGGATGTGAAAATTCTTCAAACGGAACCTGATGTGGTCACCATTTGTAAACCTGCATCTGTTCCGGTGCATCCATGTGGTCAGTATCGTAAGAACACTGTTGTTGGCATCCTACAAGCAGAGCATGGCTTGGCACCTCTATTTCCGATTCATCGGCTAGATCGTTTGGTCTCAGGACTCCTTATCTTGGCTAGAAGCGCAGCACAAGCTGACATTTTCAGGCAACAGATTGAGGGAGGAATGGTTCATAAACAGTACATTGCAAGAGTCATTGGAGAGTTTCCTGAAGAGGAGCAAGTTGTAGATGCTAACATAGATCATAATGCTCGAGAAGGAAGGAGCTCTGCCGAGGTTGGTGATTCGTGTAGTGATGCTCCCACGAAGGGGAAGACTGCTTGTACAAAGTTCACTAGAATCAGTTCAAATGGAATTCAGAGCATTGTCTTGTGTGAACCAGTCACTGGCCGCACTCATCAAATACGCGTCCATTTGCAACATGCAGGCCATCCAATAGCAAATGACATGCTTTACCTGTCCAAACATGTTAGTGATCGTTCAGCTGAAGGAATGGGTGCTGATAGAGCTGCTGCTAATTCAGACCACTCTCCGGCTTCTGTTGTTGGCAAAATTAAATCCACTTCGCATGAAGATTCTAGTGAGGACTTCAGCATTGATCCAATGTGTACAAATTGTCCAAGCTTAGCTCCAAAAGGATACGATTATCATGAAGAGGCCTTGTGGCTACATTGTTTTAGATATTCTGGACCCGGATGGGTTTATGAATGCCCACATCCGGATTGGGCATCACTTAGCTAG

Protein Analysis

384

Amino Acids

43.06

Weight (kDa)

7.04

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 146 - 295 8.2e-25 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 96, 469
AccB7I CCANNNNNTGG 1 cut(s) 797
AccBSI CCGCTC 1 cut(s) 67
AccII CGCG 1 cut(s) 819
AccIII TCCGGA 1 cut(s) 1129
AciI CCGC 5 cut(s) 59, 67, 152, 323, 802
AclWI GGATC 1 cut(s) 1004
AcoI YGGCCR 1 cut(s) 799
AcsI RAATTY 2 cut(s) 348, 765
AcuI CTGAAG 3 cut(s) 642, 918, 985
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 3 cut(s) 593, 736, 1021
AfiI CCNNNNNNNGG 6 cut(s) 523, 719, 720, 797, 943, 1129
AflIII ACRYGT 2 cut(s) 817, 877
AgsI TTSAA 3 cut(s) 190, 356, 759
AloI GAACNNNNNNTCC 2 cut(s) 564, 596
AluBI AGCT 8 cut(s) 256, 545, 678, 896, 920, 1035, 1040, 1149
AluI AGCT 8 cut(s) 256, 545, 678, 896, 920, 1035, 1040, 1149
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 3 cut(s) 14, 100, 511
AlwI GGATC 1 cut(s) 1004
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 663
Aor13HI TCCGGA 1 cut(s) 1129
AoxI GGCC 4 cut(s) 193, 799, 838, 1068
ApeKI GCWGC 4 cut(s) 253, 536, 920, 923
ApoI RAATTY 2 cut(s) 348, 765
Asp700I GAANNNNTTC 1 cut(s) 754
AspLEI GCGC 2 cut(s) 99, 536
AspS9I GGNCC 1 cut(s) 1100
AsuC2I CCSGG 1 cut(s) 1104
AsuHPI GGTGA 2 cut(s) 367, 704
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 1100
BanI GGYRCC 2 cut(s) 96, 469
BanII GRGCYC 1 cut(s) 680
BbsI GAAGAC 2 cut(s) 164, 731
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 4 cut(s) 265, 548, 907, 910
BccI CCATC 4 cut(s) 170, 242, 849, 1101
BciVI GTATCC 2 cut(s) 231, 1043
BcnI CCSGG 1 cut(s) 1104
BcoDI GTCTC 3 cut(s) 14, 100, 511
BfaI CTAG 5 cut(s) 494, 528, 747, 990, 1150
BfoI RGCGCY 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 397
BfuI GTATCC 2 cut(s) 231, 1043
BisI GCNGC 5 cut(s) 254, 537, 802, 921, 924
BlpI GCTNAGC 1 cut(s) 1036
BlsI GCNGC 5 cut(s) 255, 538, 803, 922, 925
Bme1390I CCNGG 1 cut(s) 1104
Bme18I GGWCC 1 cut(s) 1100
BmeT110I CYCGRG 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 1100
BmiI GGNNCC 4 cut(s) 98, 363, 471, 1102
BmrFI CCNGG 1 cut(s) 1104
BmsI GCATC 6 cut(s) 401, 415, 453, 631, 697, 1148
BpiI GAAGAC 2 cut(s) 164, 731
Bpu1102I GCTNAGC 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 1104
BsaHI GRCGYC 1 cut(s) 97
BsaI GGTCTC 2 cut(s) 100, 511
BsaJI CCNNGG 1 cut(s) 684
BsaWI WCCGGW 2 cut(s) 400, 1129
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 6 cut(s) 523, 719, 720, 797, 943, 1129
Bse118I RCCGGY 2 cut(s) 49, 262
Bse1I ACTGG 3 cut(s) 326, 791, 802
Bse3DI GCAATG 2 cut(s) 126, 594
BseAI TCCGGA 1 cut(s) 1129
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 6 cut(s) 346, 406, 444, 841, 1112, 1126
BseLI CCNNNNNNNGG 6 cut(s) 523, 719, 720, 797, 943, 1129
BseMI GCAATG 2 cut(s) 126, 594
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 3 cut(s) 326, 791, 802
BseRI GAGGAG 1 cut(s) 641
BseXI GCAGC 4 cut(s) 265, 548, 907, 910
BsgI GTGCAG 2 cut(s) 240, 293
Bsh1236I CGCG 1 cut(s) 819
BshFI GGCC 4 cut(s) 195, 801, 840, 1070
BshNI GGYRCC 2 cut(s) 96, 469
BsiHKAI GWGCWC 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 8 cut(s) 50, 62, 196, 263, 401, 944, 1104, 1130
BslI CCNNNNNNNGG 6 cut(s) 523, 719, 720, 797, 943, 1129
BsmAI GTCTC 3 cut(s) 14, 100, 511
BsmBI CGTCTC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 4 cut(s) 195, 801, 840, 1070
Bso31I GGTCTC 2 cut(s) 100, 511
BsoBI CYCGRG 1 cut(s) 663
Bsp1286I GDGCHC 1 cut(s) 680
Bsp13I TCCGGA 1 cut(s) 1129
Bsp1407I TGTACA 2 cut(s) 734, 1019
Bsp143I GATC 5 cut(s) 33, 496, 652, 886, 1009
Bsp1720I GCTNAGC 1 cut(s) 1036
BspACI CCGC 5 cut(s) 59, 67, 152, 323, 802
BspANI GGCC 4 cut(s) 195, 801, 840, 1070
BspCNI CTCAG 1 cut(s) 521
BspEI TCCGGA 1 cut(s) 1129
BspFNI CGCG 1 cut(s) 819
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 4 cut(s) 98, 363, 471, 1102
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 1004
BspT107I GGYRCC 2 cut(s) 96, 469
BspTNI GGTCTC 2 cut(s) 100, 511
BsrBI CCGCTC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 126, 594
BsrFI RCCGGY 2 cut(s) 49, 262
BsrGI TGTACA 2 cut(s) 734, 1019
BsrI ACTGG 3 cut(s) 326, 791, 802
BssAI RCCGGY 2 cut(s) 49, 262
BssECI CCNNGG 1 cut(s) 684
BssMI GATC 5 cut(s) 33, 496, 652, 886, 1009
BssNI GRCGYC 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 285, 436, 591
Bst6I CTCTTC 3 cut(s) 12, 618, 1059
BstACI GRCGYC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 259
BstAUI TGTACA 2 cut(s) 734, 1019
BstC8I GCNNGC 2 cut(s) 51, 838
BstDEI CTNAG 3 cut(s) 508, 1036, 1145
BstEII GGTNACC 1 cut(s) 373
BstF5I GGATG 6 cut(s) 346, 406, 444, 841, 1112, 1126
BstFNI CGCG 1 cut(s) 819
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 2 cut(s) 99, 536
BstKTI GATC 5 cut(s) 36, 499, 655, 889, 1012
BstMAI GTCTC 3 cut(s) 14, 100, 511
BstMBI GATC 5 cut(s) 33, 496, 652, 886, 1009
BstMWI GCNNNNNNNGC 5 cut(s) 259, 322, 533, 542, 917
BstNSI RCATGY 3 cut(s) 836, 862, 881
BstPI GGTNACC 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 1102
BstUI CGCG 1 cut(s) 819
BstV1I GCAGC 4 cut(s) 265, 548, 907, 910
BstV2I GAAGAC 2 cut(s) 164, 731
BsuI GTATCC 2 cut(s) 231, 1043
BsuRI GGCC 4 cut(s) 195, 801, 840, 1070
BtsCI GGATG 6 cut(s) 346, 406, 444, 841, 1112, 1126
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 5 cut(s) 177, 228, 333, 432, 795
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 51, 838
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 1 cut(s) 1060
CfoI GCGC 2 cut(s) 99, 536
Cfr10I RCCGGY 2 cut(s) 49, 262
Cfr13I GGNCC 1 cut(s) 1100
CseI GACGC 1 cut(s) 808
Csp6I GTAC 3 cut(s) 592, 735, 1020
CviQI GTAC 3 cut(s) 592, 735, 1020
DdeI CTNAG 3 cut(s) 508, 1036, 1145
DinI GGCGCC 1 cut(s) 98
DpnI GATC 5 cut(s) 35, 498, 654, 888, 1011
DpnII GATC 5 cut(s) 33, 496, 652, 886, 1009
EaeI YGGCCR 1 cut(s) 799
Eam1104I CTCTTC 3 cut(s) 12, 618, 1059
EarI CTCTTC 3 cut(s) 12, 618, 1059
Ecl136II GAGCTC 1 cut(s) 678
Eco147I AGGCCT 1 cut(s) 1070
Eco24I GRGCYC 1 cut(s) 680
Eco31I GGTCTC 2 cut(s) 100, 511
Eco47I GGWCC 1 cut(s) 1100
Eco53kI GAGCTC 1 cut(s) 678
Eco57I CTGAAG 3 cut(s) 642, 918, 985
Eco88I CYCGRG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 678
EcoO65I GGTNACC 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 765
EcoT38I GRGCYC 1 cut(s) 680
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
Esp3I CGTCTC 1 cut(s) 14
FauI CCCGC 2 cut(s) 66, 145
Fnu4HI GCNGC 5 cut(s) 254, 537, 802, 921, 924
FokI GGATG 6 cut(s) 353, 393, 431, 828, 1113, 1119
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 5 cut(s) 254, 537, 802, 921, 924
FspBI CTAG 5 cut(s) 494, 528, 747, 990, 1150
GlaI GCGC 2 cut(s) 98, 535
GluI GCNGC 5 cut(s) 254, 537, 802, 921, 924
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 4 cut(s) 195, 801, 840, 1070
HapII CCGG 8 cut(s) 50, 62, 196, 263, 401, 944, 1104, 1130
HgaI GACGC 1 cut(s) 808
HhaI GCGC 2 cut(s) 99, 536
Hin1I GRCGYC 1 cut(s) 97
Hin6I GCGC 2 cut(s) 97, 534
HinP1I GCGC 2 cut(s) 97, 534
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 7 cut(s) 199, 484, 513, 603, 695, 750, 986
HpaII CCGG 8 cut(s) 50, 62, 196, 263, 401, 944, 1104, 1130
HphI GGTGA 2 cut(s) 367, 704
Hpy166II GTNNAC 6 cut(s) 169, 177, 386, 744, 788, 1020
Hpy188I TCNGA 3 cut(s) 483, 771, 934
Hpy188III TCNNGA 7 cut(s) 203, 510, 620, 665, 1061, 1098, 1130
Hpy8I GTNNAC 6 cut(s) 169, 177, 386, 744, 788, 1020
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 4 cut(s) 662, 666, 712, 893
HpyCH4III ACNGT 3 cut(s) 285, 436, 591
HpyCH4V TGCA 7 cut(s) 221, 310, 392, 406, 599, 829, 836
HpyF10VI GCNNNNNNNGC 5 cut(s) 259, 322, 533, 542, 917
HpyF3I CTNAG 3 cut(s) 508, 1036, 1145
Hsp92I GRCGYC 1 cut(s) 97
HspAI GCGC 2 cut(s) 97, 534
KasI GGCGCC 1 cut(s) 96
Kpn2I TCCGGA 1 cut(s) 1129
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Kzo9I GATC 5 cut(s) 33, 496, 652, 886, 1009
LmnI GCTCC 5 cut(s) 64, 628, 675, 715, 1045
Lsp1109I GCAGC 4 cut(s) 265, 548, 907, 910
LweI GCATC 6 cut(s) 401, 415, 453, 631, 697, 1148
MaeI CTAG 5 cut(s) 494, 528, 747, 990, 1150
MaeIII GTNAC 3 cut(s) 203, 373, 793
MalI GATC 5 cut(s) 35, 498, 654, 888, 1011
MbiI CCGCTC 1 cut(s) 67
MboI GATC 5 cut(s) 33, 496, 652, 886, 1009
MboII GAAGA 9 cut(s) 16, 22, 29, 169, 344, 635, 736, 995, 1076
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 5 cut(s) 348, 765, 928, 963, 1024
MluI ACGCGT 1 cut(s) 817
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 2 cut(s) 507, 612
MnlI CCTC 9 cut(s) 87, 228, 483, 562, 566, 619, 679, 988, 1060
MreI CGCCGGCG 1 cut(s) 49
MroI TCCGGA 1 cut(s) 1129
MroNI GCCGGC 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 3 cut(s) 138, 279, 966
MslI CAYNNNNRTG 3 cut(s) 315, 411, 882
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 8 cut(s) 50, 62, 196, 263, 401, 944, 1104, 1130
MspR9I CCNGG 1 cut(s) 1104
Mva1269I GAATGC 1 cut(s) 1124
MvnI CGCG 1 cut(s) 819
MwoI GCNNNNNNNGC 5 cut(s) 259, 322, 533, 542, 917
NaeI GCCGGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 97
NciI CCSGG 1 cut(s) 1104
NdeII GATC 5 cut(s) 33, 496, 652, 886, 1009
NgoMIV GCCGGC 1 cut(s) 49
NlaIV GGNNCC 4 cut(s) 98, 363, 471, 1102
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 3 cut(s) 203, 373, 793
NspI RCATGY 3 cut(s) 836, 862, 881
PaeR7I CTCGAG 1 cut(s) 663
PagI TCATGA 1 cut(s) 1060
PceI AGGCCT 1 cut(s) 1070
PciI ACATGT 1 cut(s) 877
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PctI GAATGC 1 cut(s) 1124
PdiI GCCGGC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 5 cut(s) 199, 484, 695, 750, 986
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 5 cut(s) 255, 538, 803, 922, 925
PleI GAGTC 2 cut(s) 507, 611
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 2 cut(s) 507, 611
PscI ACATGT 1 cut(s) 877
Psp124BI GAGCTC 1 cut(s) 680
PspEI GGTNACC 1 cut(s) 373
PspN4I GGNNCC 4 cut(s) 98, 363, 471, 1102
PspPI GGNCC 1 cut(s) 1100
PstNI CAGNNNCTG 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 896
RsaI GTAC 3 cut(s) 593, 736, 1021
RsaNI GTAC 3 cut(s) 592, 735, 1020
RseI CAYNNNNRTG 3 cut(s) 315, 411, 882
SacI GAGCTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 138, 279, 966
SatI GCNGC 5 cut(s) 254, 537, 802, 921, 924
Sau3AI GATC 5 cut(s) 33, 496, 652, 886, 1009
Sau96I GGNCC 1 cut(s) 1100
SchI GAGTC 2 cut(s) 507, 612
ScrFI CCNGG 1 cut(s) 1104
SduI GDGCHC 1 cut(s) 680
SfaNI GCATC 6 cut(s) 401, 415, 453, 631, 697, 1148
SfoI GGCGCC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 49
SinI GGWCC 1 cut(s) 1100
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 3 cut(s) 315, 411, 882
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 5 cut(s) 348, 765, 928, 963, 1024
SseBI AGGCCT 1 cut(s) 1070
SsiI CCGC 5 cut(s) 59, 67, 152, 323, 802
SspDI GGCGCC 1 cut(s) 96
SspMI CTAG 5 cut(s) 494, 528, 747, 990, 1150
SstI GAGCTC 1 cut(s) 680
StuI AGGCCT 1 cut(s) 1070
StyD4I CCNGG 1 cut(s) 1102
TaaI ACNGT 3 cut(s) 285, 436, 591
TaqI TCGA 1 cut(s) 664
TasI AATT 5 cut(s) 348, 765, 928, 963, 1024
TatI WGTACW 3 cut(s) 591, 734, 1019
TauI GCSGC 1 cut(s) 804
TfiI GAWTC 5 cut(s) 199, 484, 695, 750, 986
Tru1I TTAA 3 cut(s) 138, 279, 966
Tru9I TTAA 3 cut(s) 138, 279, 966
TscAI CASTG 5 cut(s) 184, 228, 333, 439, 802
TseFI GTSAC 3 cut(s) 203, 373, 793
TseI GCWGC 4 cut(s) 253, 536, 920, 923
Tsp45I GTSAC 3 cut(s) 203, 373, 793
TspGWI ACGGA 2 cut(s) 38, 374
TspRI CASTG 5 cut(s) 184, 228, 333, 439, 802
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 1100
XapI RAATTY 2 cut(s) 348, 765
XceI RCATGY 3 cut(s) 836, 862, 881
XcmI CCANNNNNNNNNTGG 2 cut(s) 333, 1131
XhoI CTCGAG 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 5 cut(s) 494, 528, 747, 990, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.