Rh7CG106500

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0009164
Physical Location & Seq
Reverse (-)
8158806 .. 8162000
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG106500.1

Sequence Viewer

Length: 1152 bp
ATGAAGAGGAAGTTAGAAGAGACGGACATGGAGATCGTCTGGCAATCGCCGGCGAACCCTCCGGAACGGAAAGATTACATCTTTCGCAACGGGAGGCGCCATGTGAGACCCTACTACTTTGAGTTCATTGCTCATGTTAAGAACCGATGGGCGGGGAAGACCATCGTTGACTTGTTCACTGACGAGTTCAAAGGCCGGAATCGTGACTACTATATTAGTGCAGTTAAATGTGGGAGGATACAAGTTGATGGGCAACTTGTGCCGGTTTCCTTCATAGTTAAACCGTCGCAAAAGATAAGCCACTTCCTGCACAGGCATGAACCGCCCGTGATGACTTGGGATGTGAAAATTCTTGAAACAGAACCCGATGTGGTCACCATTTGTAAACCTGCATCTGTTCCAGTGCATCCATGTGGTCAGTATCGTAAGAACACTGTTGTTGGCATCCTACAGGCAGAGCATGGCTTGGCACCTCTATTTCCGATTCATCGACTAGACCGTCTGGTCTCAGGACTCCTTATCTTGGCTAGAAGCGCAGCACAAGCTGACATTTTCAGGCAACAGATTGAGGGAGGGATGGTTCATAAACAGTACATTGCAAGAGTCATTGGAGAGTTTCCTGAGGAGGAGCAAGTTGTAGATGCTAACATAAATCATAATGCTCGAGAAGGAAGGAGCTCTGCTGAGGTCGGTGATTCTTGTGGTGATGCTCCCACAAAGGGGAAGACTGCTTGTACAAAGTTTACTAGAATCAGTACAAATGGAATTCAGAGCATTGTCTTGTGTGAACCAGTCACTGGCCGCACTCATCAGATACGCGTCCATTTGCAACATGCAGGCCATCCAATAGCGAATGACATGCTTTACCTGTCCAAACGTGTAAGTGATCGTTCAGCTGAAGGAATGGGTGCTGATAGAGCTGCTGCTAATCCAGACCACTCTCCAGCTTCTGGTGTTGGCGAAAATAAATCCGCTTTGCATGAAGATTCTGGTGAGGACTTCAGCATTGATCCAATGTGTACAAATTGTCCAAGTTTAGCTCCAAAAGGATACGATTATCATGAAGAGGCCTTGTGGCTACACTGTTTTAGATATTCTGGACCTGGATGGGTTTATGAGTGCCCACATCCGGATTGGGCATCACTTAGCTAG

Protein Analysis

383

Amino Acids

43.0

Weight (kDa)

6.83

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 123 - 278 1.5e-28 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 498, 503
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 96, 469
AccB7I CCANNNNNTGG 2 cut(s) 797, 950
AccII CGCG 1 cut(s) 819
AccIII TCCGGA 2 cut(s) 61, 1129
AciI CCGC 4 cut(s) 152, 323, 802, 972
AclWI GGATC 1 cut(s) 1004
AcoI YGGCCR 1 cut(s) 799
AcsI RAATTY 2 cut(s) 348, 765
AcuI CTGAAG 2 cut(s) 918, 985
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 4 cut(s) 593, 736, 757, 1021
AfiI CCNNNNNNNGG 7 cut(s) 151, 523, 719, 720, 797, 950, 1129
AflIII ACRYGT 2 cut(s) 817, 877
AgsI TTSAA 2 cut(s) 190, 356
AjnI CCWGG 1 cut(s) 1102
AloI GAACNNNNNNTCC 2 cut(s) 564, 596
AluBI AGCT 7 cut(s) 545, 678, 896, 920, 947, 1040, 1149
AluI AGCT 7 cut(s) 545, 678, 896, 920, 947, 1040, 1149
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 3 cut(s) 14, 100, 511
AlwI GGATC 1 cut(s) 1004
AlwNI CAGNNNCTG 2 cut(s) 797, 950
Ama87I CYCGRG 1 cut(s) 663
Aor13HI TCCGGA 2 cut(s) 61, 1129
AoxI GGCC 4 cut(s) 193, 799, 838, 1068
ApeKI GCWGC 3 cut(s) 536, 920, 923
ApoI RAATTY 2 cut(s) 348, 765
AspLEI GCGC 2 cut(s) 99, 536
AspS9I GGNCC 1 cut(s) 1100
AsuHPI GGTGA 4 cut(s) 367, 704, 716, 1004
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 1100
AxyI CCTNAGG 1 cut(s) 621
BaeGI GKGCMC 1 cut(s) 1124
BanI GGYRCC 2 cut(s) 96, 469
BanII GRGCYC 1 cut(s) 680
BbsI GAAGAC 2 cut(s) 164, 731
Bbv12I GWGCWC 1 cut(s) 680
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 548, 907, 910
BccI CCATC 6 cut(s) 141, 170, 242, 571, 849, 1101
BcgI CGANNNNNNTGC 2 cut(s) 841, 875
BciT130I CCWGG 1 cut(s) 1104
BciVI GTATCC 2 cut(s) 231, 1043
BcoDI GTCTC 3 cut(s) 14, 100, 511
BfaI CTAG 4 cut(s) 494, 528, 747, 1150
BfmI CTRYAG 1 cut(s) 449
BfoI RGCGCY 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 397
BfuI GTATCC 2 cut(s) 231, 1043
BisI GCNGC 4 cut(s) 537, 802, 921, 924
BlsI GCNGC 4 cut(s) 538, 803, 922, 925
Bme1390I CCNGG 1 cut(s) 1104
Bme18I GGWCC 1 cut(s) 1100
BmeT110I CYCGRG 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 1100
BmiI GGNNCC 2 cut(s) 98, 471
BmrFI CCNGG 1 cut(s) 1104
BmsI GCATC 6 cut(s) 401, 415, 453, 631, 697, 1148
BpiI GAAGAC 2 cut(s) 164, 731
BpmI CTGGAG 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 97
BsaI GGTCTC 2 cut(s) 100, 511
BsaWI WCCGGW 2 cut(s) 61, 1129
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 7 cut(s) 151, 523, 719, 720, 797, 950, 1129
Bse118I RCCGGY 2 cut(s) 49, 262
Bse1I ACTGG 3 cut(s) 401, 791, 802
Bse21I CCTNAGG 1 cut(s) 621
Bse3DI GCAATG 2 cut(s) 126, 594
BseAI TCCGGA 2 cut(s) 61, 1129
BseBI CCWGG 1 cut(s) 1104
BseGI GGATG 7 cut(s) 346, 406, 444, 582, 841, 1112, 1126
BseLI CCNNNNNNNGG 7 cut(s) 151, 523, 719, 720, 797, 950, 1129
BseMI GCAATG 2 cut(s) 126, 594
BseMII CTCAG 3 cut(s) 522, 612, 675
BseNI ACTGG 3 cut(s) 401, 791, 802
BseRI GAGGAG 2 cut(s) 638, 641
BseSI GKGCMC 1 cut(s) 1124
BseXI GCAGC 3 cut(s) 548, 907, 910
BsgI GTGCAG 2 cut(s) 240, 293
Bsh1236I CGCG 1 cut(s) 819
BshFI GGCC 4 cut(s) 195, 801, 840, 1070
BshNI GGYRCC 2 cut(s) 96, 469
BsiHKAI GWGCWC 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 5 cut(s) 50, 62, 196, 263, 1130
BslI CCNNNNNNNGG 7 cut(s) 151, 523, 719, 720, 797, 950, 1129
BsmAI GTCTC 3 cut(s) 14, 100, 511
BsmBI CGTCTC 1 cut(s) 14
BsnI GGCC 4 cut(s) 195, 801, 840, 1070
Bso31I GGTCTC 2 cut(s) 100, 511
BsoBI CYCGRG 1 cut(s) 663
Bsp1286I GDGCHC 2 cut(s) 680, 1124
Bsp13I TCCGGA 2 cut(s) 61, 1129
Bsp1407I TGTACA 2 cut(s) 734, 1019
Bsp143I GATC 3 cut(s) 33, 886, 1009
BspACI CCGC 4 cut(s) 152, 323, 802, 972
BspANI GGCC 4 cut(s) 195, 801, 840, 1070
BspCNI CTCAG 3 cut(s) 521, 613, 676
BspEI TCCGGA 2 cut(s) 61, 1129
BspFNI CGCG 1 cut(s) 819
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 2 cut(s) 98, 471
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 1004
BspT107I GGYRCC 2 cut(s) 96, 469
BspTNI GGTCTC 2 cut(s) 100, 511
BsrDI GCAATG 2 cut(s) 126, 594
BsrFI RCCGGY 2 cut(s) 49, 262
BsrGI TGTACA 2 cut(s) 734, 1019
BsrI ACTGG 3 cut(s) 401, 791, 802
BssAI RCCGGY 2 cut(s) 49, 262
BssMI GATC 3 cut(s) 33, 886, 1009
BssNI GRCGYC 1 cut(s) 97
Bst2UI CCWGG 1 cut(s) 1104
Bst4CI ACNGT 5 cut(s) 285, 436, 500, 591, 1085
Bst6I CTCTTC 2 cut(s) 12, 1059
BstACI GRCGYC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 259
BstAUI TGTACA 2 cut(s) 734, 1019
BstC8I GCNNGC 2 cut(s) 51, 838
BstDEI CTNAG 4 cut(s) 508, 621, 684, 1145
BstEII GGTNACC 1 cut(s) 373
BstF5I GGATG 7 cut(s) 346, 406, 444, 582, 841, 1112, 1126
BstFNI CGCG 1 cut(s) 819
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 2 cut(s) 99, 536
BstKTI GATC 3 cut(s) 36, 889, 1012
BstMAI GTCTC 3 cut(s) 14, 100, 511
BstMBI GATC 3 cut(s) 33, 886, 1009
BstMWI GCNNNNNNNGC 5 cut(s) 259, 322, 533, 542, 917
BstNI CCWGG 1 cut(s) 1104
BstNSI RCATGY 2 cut(s) 836, 862
BstPI GGTNACC 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 1102
BstSFI CTRYAG 1 cut(s) 449
BstSLI GKGCMC 1 cut(s) 1124
BstUI CGCG 1 cut(s) 819
BstV1I GCAGC 3 cut(s) 548, 907, 910
BstV2I GAAGAC 2 cut(s) 164, 731
Bsu36I CCTNAGG 1 cut(s) 621
BsuI GTATCC 2 cut(s) 231, 1043
BsuRI GGCC 4 cut(s) 195, 801, 840, 1070
BtsCI GGATG 7 cut(s) 346, 406, 444, 582, 841, 1112, 1126
BtsIMutI CAGTG 5 cut(s) 177, 408, 432, 795, 1081
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 51, 838
CaiI CAGNNNCTG 2 cut(s) 797, 950
CciI TCATGA 1 cut(s) 1060
CfoI GCGC 2 cut(s) 99, 536
Cfr10I RCCGGY 2 cut(s) 49, 262
Cfr13I GGNCC 1 cut(s) 1100
CseI GACGC 1 cut(s) 808
Csp6I GTAC 4 cut(s) 592, 735, 756, 1020
CviQI GTAC 4 cut(s) 592, 735, 756, 1020
DdeI CTNAG 4 cut(s) 508, 621, 684, 1145
DinI GGCGCC 1 cut(s) 98
DpnI GATC 3 cut(s) 35, 888, 1011
DpnII GATC 3 cut(s) 33, 886, 1009
DrdI GACNNNNNNGTC 2 cut(s) 498, 503
DseDI GACNNNNNNGTC 2 cut(s) 498, 503
EaeI YGGCCR 1 cut(s) 799
Eam1104I CTCTTC 2 cut(s) 12, 1059
EarI CTCTTC 2 cut(s) 12, 1059
Ecl136II GAGCTC 1 cut(s) 678
Eco147I AGGCCT 1 cut(s) 1070
Eco24I GRGCYC 1 cut(s) 680
Eco31I GGTCTC 2 cut(s) 100, 511
Eco47I GGWCC 1 cut(s) 1100
Eco53kI GAGCTC 1 cut(s) 678
Eco57I CTGAAG 2 cut(s) 918, 985
Eco81I CCTNAGG 1 cut(s) 621
Eco88I CYCGRG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 678
EcoO65I GGTNACC 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 1102
EcoT38I GRGCYC 1 cut(s) 680
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
Esp3I CGTCTC 1 cut(s) 14
FauI CCCGC 1 cut(s) 145
Fnu4HI GCNGC 4 cut(s) 537, 802, 921, 924
FokI GGATG 7 cut(s) 353, 393, 431, 589, 828, 1113, 1119
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 4 cut(s) 537, 802, 921, 924
FspBI CTAG 4 cut(s) 494, 528, 747, 1150
GlaI GCGC 2 cut(s) 98, 535
GluI GCNGC 4 cut(s) 537, 802, 921, 924
GsuI CTGGAG 1 cut(s) 927
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 4 cut(s) 195, 801, 840, 1070
HapII CCGG 5 cut(s) 50, 62, 196, 263, 1130
HgaI GACGC 1 cut(s) 808
HhaI GCGC 2 cut(s) 99, 536
Hin1I GRCGYC 1 cut(s) 97
Hin6I GCGC 2 cut(s) 97, 534
HinP1I GCGC 2 cut(s) 97, 534
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 7 cut(s) 199, 484, 513, 603, 695, 750, 986
HpaII CCGG 5 cut(s) 50, 62, 196, 263, 1130
HphI GGTGA 4 cut(s) 367, 704, 716, 1004
Hpy166II GTNNAC 6 cut(s) 169, 177, 386, 744, 788, 1020
Hpy188I TCNGA 3 cut(s) 483, 771, 813
Hpy8I GTNNAC 6 cut(s) 169, 177, 386, 744, 788, 1020
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 4 cut(s) 280, 662, 666, 893
HpyCH4III ACNGT 5 cut(s) 285, 436, 500, 591, 1085
HpyCH4IV ACGT 1 cut(s) 877
HpyCH4V TGCA 8 cut(s) 221, 310, 392, 406, 599, 829, 836, 979
HpyF10VI GCNNNNNNNGC 5 cut(s) 259, 322, 533, 542, 917
HpyF3I CTNAG 4 cut(s) 508, 621, 684, 1145
HpySE526I ACGT 1 cut(s) 877
Hsp92I GRCGYC 1 cut(s) 97
HspAI GCGC 2 cut(s) 97, 534
KasI GGCGCC 1 cut(s) 96
Kpn2I TCCGGA 2 cut(s) 61, 1129
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Kzo9I GATC 3 cut(s) 33, 886, 1009
LmnI GCTCC 4 cut(s) 628, 675, 715, 1045
Lsp1109I GCAGC 3 cut(s) 548, 907, 910
LweI GCATC 6 cut(s) 401, 415, 453, 631, 697, 1148
MaeI CTAG 4 cut(s) 494, 528, 747, 1150
MaeII ACGT 1 cut(s) 877
MaeIII GTNAC 3 cut(s) 203, 373, 793
MalI GATC 3 cut(s) 35, 888, 1011
MboI GATC 3 cut(s) 33, 886, 1009
MboII GAAGA 6 cut(s) 16, 29, 169, 736, 995, 1076
MhlI GDGCHC 2 cut(s) 680, 1124
MluCI AATT 3 cut(s) 348, 765, 1024
MluI ACGCGT 1 cut(s) 817
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 2 cut(s) 507, 612
MreI CGCCGGCG 1 cut(s) 49
MroI TCCGGA 2 cut(s) 61, 1129
MroNI GCCGGC 1 cut(s) 49
MseI TTAA 3 cut(s) 138, 225, 279
MslI CAYNNNNRTG 2 cut(s) 315, 411
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 5 cut(s) 50, 62, 196, 263, 1130
MspR9I CCNGG 1 cut(s) 1104
MvaI CCWGG 1 cut(s) 1104
MvnI CGCG 1 cut(s) 819
MwoI GCNNNNNNNGC 5 cut(s) 259, 322, 533, 542, 917
NaeI GCCGGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 97
NdeII GATC 3 cut(s) 33, 886, 1009
NgoMIV GCCGGC 1 cut(s) 49
NlaIV GGNNCC 2 cut(s) 98, 471
NmuCI GTSAC 3 cut(s) 203, 373, 793
NspI RCATGY 2 cut(s) 836, 862
PaeR7I CTCGAG 1 cut(s) 663
PagI TCATGA 1 cut(s) 1060
PceI AGGCCT 1 cut(s) 1070
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PdiI GCCGGC 1 cut(s) 51
PfeI GAWTC 5 cut(s) 199, 484, 695, 750, 986
PflMI CCANNNNNTGG 2 cut(s) 797, 950
PkrI GCNGC 4 cut(s) 538, 803, 922, 925
PleI GAGTC 2 cut(s) 507, 611
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 2 cut(s) 507, 611
Psp124BI GAGCTC 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 1102
PspEI GGTNACC 1 cut(s) 373
PspGI CCWGG 1 cut(s) 1102
PspN4I GGNNCC 2 cut(s) 98, 471
PspPI GGNCC 1 cut(s) 1100
PstNI CAGNNNCTG 2 cut(s) 797, 950
PvuII CAGCTG 1 cut(s) 896
RsaI GTAC 4 cut(s) 593, 736, 757, 1021
RsaNI GTAC 4 cut(s) 592, 735, 756, 1020
RseI CAYNNNNRTG 2 cut(s) 315, 411
SacI GAGCTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 138, 225, 279
SatI GCNGC 4 cut(s) 537, 802, 921, 924
Sau3AI GATC 3 cut(s) 33, 886, 1009
Sau96I GGNCC 1 cut(s) 1100
SchI GAGTC 2 cut(s) 507, 612
ScrFI CCNGG 1 cut(s) 1104
SduI GDGCHC 2 cut(s) 680, 1124
SfaNI GCATC 6 cut(s) 401, 415, 453, 631, 697, 1148
SfcI CTRYAG 1 cut(s) 449
SfoI GGCGCC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 49
SinI GGWCC 1 cut(s) 1100
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 2 cut(s) 315, 411
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 3 cut(s) 348, 765, 1024
SseBI AGGCCT 1 cut(s) 1070
SsiI CCGC 4 cut(s) 152, 323, 802, 972
SspDI GGCGCC 1 cut(s) 96
SspMI CTAG 4 cut(s) 494, 528, 747, 1150
SstI GAGCTC 1 cut(s) 680
StuI AGGCCT 1 cut(s) 1070
StyD4I CCNGG 1 cut(s) 1102
TaaI ACNGT 5 cut(s) 285, 436, 500, 591, 1085
TaiI ACGT 1 cut(s) 880
TaqI TCGA 2 cut(s) 490, 664
TasI AATT 3 cut(s) 348, 765, 1024
TatI WGTACW 4 cut(s) 591, 734, 755, 1019
TauI GCSGC 1 cut(s) 804
TfiI GAWTC 5 cut(s) 199, 484, 695, 750, 986
Tru1I TTAA 3 cut(s) 138, 225, 279
Tru9I TTAA 3 cut(s) 138, 225, 279
TscAI CASTG 5 cut(s) 184, 408, 439, 802, 1088
TseFI GTSAC 3 cut(s) 203, 373, 793
TseI GCWGC 3 cut(s) 536, 920, 923
Tsp45I GTSAC 3 cut(s) 203, 373, 793
TspDTI ATGAA 8 cut(s) 17, 115, 262, 333, 476, 572, 996, 1077
TspGWI ACGGA 2 cut(s) 38, 82
TspRI CASTG 5 cut(s) 184, 408, 439, 802, 1088
Van91I CCANNNNNTGG 2 cut(s) 797, 950
VpaK11BI GGWCC 1 cut(s) 1100
XapI RAATTY 2 cut(s) 348, 765
XceI RCATGY 2 cut(s) 836, 862
XcmI CCANNNNNNNNNTGG 1 cut(s) 1131
XhoI CTCGAG 1 cut(s) 663
XspI CTAG 4 cut(s) 494, 528, 747, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.