RchiOBHm_Chr7g0189351

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009164
Physical Location & Seq
Reverse (-)
8675523 .. 8679026
3504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16909

Sequence Viewer

Length: 1230 bp
ATGCCAACGACGTCGTTTGCGTCTAGGGCTCTGCAAATTTGCAAAAATCTGATCCCAAATCGGAATAAAAGAGGAAAAATGAAGAGGAAGATAGAAGAGACGGACATGGAGATCGTCTGGCAATCGCCGGCGAACCCTCCGGAACGGAAAGATTACATCTTTCGCAACGGGAGGCGCCATGTGAGACCCTACTACTTTGAGTTCATTGCTCATGTTAAGAAGCGGTGGGCGGGGAAGACCATCGTTGACTTGTTCACTGACGAGTTCAAAGGCCGGAATCGTGACTACTATATTAGTGCGGTTAAATGTGGGAGGATACAAGTTGATGGGCAACTTGTGCCGGTTTCCTTCATAGTTAAACCGTCGCAAAAGATAAGCCACTTCCTGCACAGGCATGAACCGCCCGTGATGACTTGGGATGTGAAAATTCTTGAAACAGAACCCGATGTGGTCACCATTTGTAAACCTGCATCTGTTCCAGTGCATCCATGTGGTCAGTATCGTAAGAACACTGTTGTTGGCATCCTACAGGCAGAGCATGGCTTGGCACCTCTATTTCCGATTCATCGATTAGATCGTCTGGTCTCAGGACTCCTTATCTTGGCTAGAAGCGCAGCACAAGCTGACATTTTCAGGCAACAGATTGAGGGAGGGATGGTTCATAAACAGTACATTGCAAGAGTCATTGGAGAGTTTCCTGAGAAGGAGCAAGTTGTAGATGCTAACATAAATCATAATGCTCGAGAAGGAAGGAGCTCTACCGAGGTCGGTGATTCTTGTGGTGATGCTCCCACAAAGGGGAAGACTGCTTGTACAAAGTTTACTAGAATCAGTACGAATGGAATTCAGAGCATTGTCTTGTGTGAACCAGTCACTGGCCGCACTCATCAGATACGCGTCCATTTGCAACATGCAGGCCATCCAATAGCGAATGACATGCTTTACCTGTCCAAACATGTAAGTGATCGTTCAGCTGAAGGAATGGGTGCTGATAGAGCTGCTGCTAATCCAGACCACTCTCCAGCTTCTGGTGTTGGCGAAAATAAATCCGCTTCGCATGAAGATTCTGGTGAGGACTTCAGCATTGATCCAATGTGTACAAATTGTCCAAGTTTAGCTCCAAAAGGATACGATTATCATGAAGAGGCCTTGTGGCTACACTGTTTTAGATATTCTGGACCTGGATGGGTTTATGAGTGCCCACATCCGGGTTGGGCATCACTTAGCTAG

Protein Analysis

409

Amino Acids

45.86

Weight (kDa)

8.77

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 149 - 304 1.8e-28 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 475
AccB1I GGYRCC 2 cut(s) 174, 547
AccB7I CCANNNNNTGG 2 cut(s) 875, 1028
AccII CGCG 1 cut(s) 897
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 6 cut(s) 223, 230, 299, 401, 880, 1050
AclWI GGATC 2 cut(s) 46, 1082
AcoI YGGCCR 1 cut(s) 877
AcsI RAATTY 3 cut(s) 36, 426, 843
AcuI CTGAAG 2 cut(s) 996, 1063
AcyI GRCGYC 2 cut(s) 11, 175
AfaI GTAC 4 cut(s) 671, 814, 835, 1099
AfiI CCNNNNNNNGG 7 cut(s) 601, 797, 798, 875, 1028, 1207, 1208
AflIII ACRYGT 2 cut(s) 895, 955
AgsI TTSAA 2 cut(s) 268, 434
AjnI CCWGG 1 cut(s) 1180
AloI GAACNNNNNNTCC 2 cut(s) 642, 674
AluBI AGCT 7 cut(s) 623, 756, 974, 998, 1025, 1118, 1227
AluI AGCT 7 cut(s) 623, 756, 974, 998, 1025, 1118, 1227
Alw21I GWGCWC 1 cut(s) 758
Alw26I GTCTC 3 cut(s) 92, 178, 589
AlwI GGATC 2 cut(s) 46, 1082
AlwNI CAGNNNCTG 2 cut(s) 875, 1028
Ama87I CYCGRG 1 cut(s) 741
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 4 cut(s) 271, 877, 916, 1146
ApeKI GCWGC 3 cut(s) 614, 998, 1001
ApoI RAATTY 3 cut(s) 36, 426, 843
AspLEI GCGC 2 cut(s) 177, 614
AspS9I GGNCC 1 cut(s) 1178
AsuC2I CCSGG 1 cut(s) 1209
AsuHPI GGTGA 4 cut(s) 445, 782, 794, 1082
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 1 cut(s) 1178
BaeGI GKGCMC 1 cut(s) 1202
BanI GGYRCC 2 cut(s) 174, 547
BanII GRGCYC 2 cut(s) 31, 758
BarI GAAGNNNNNNTAC 2 cut(s) 742, 774
BbsI GAAGAC 2 cut(s) 242, 809
Bbv12I GWGCWC 1 cut(s) 758
BbvI GCAGC 3 cut(s) 626, 985, 988
BccI CCATC 5 cut(s) 248, 320, 649, 927, 1179
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BciT130I CCWGG 1 cut(s) 1182
BciVI GTATCC 2 cut(s) 309, 1121
BcnI CCSGG 1 cut(s) 1209
BcoDI GTCTC 3 cut(s) 92, 178, 589
BfaI CTAG 4 cut(s) 24, 606, 825, 1228
BfmI CTRYAG 1 cut(s) 527
BfoI RGCGCY 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 475
BfuI GTATCC 2 cut(s) 309, 1121
BisI GCNGC 4 cut(s) 615, 880, 999, 1002
BlsI GCNGC 4 cut(s) 616, 881, 1000, 1003
Bme1390I CCNGG 2 cut(s) 1182, 1209
Bme18I GGWCC 1 cut(s) 1178
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 1 cut(s) 1178
BmiI GGNNCC 2 cut(s) 176, 549
BmrFI CCNGG 2 cut(s) 1182, 1209
BmsI GCATC 6 cut(s) 479, 493, 531, 709, 775, 1226
BpiI GAAGAC 2 cut(s) 242, 809
BpmI CTGGAG 1 cut(s) 1005
BpuMI CCSGG 1 cut(s) 1209
Bsa29I ATCGAT 1 cut(s) 568
BsaHI GRCGYC 2 cut(s) 11, 175
BsaI GGTCTC 2 cut(s) 178, 589
BsaJI CCNNGG 1 cut(s) 762
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 7 cut(s) 601, 797, 798, 875, 1028, 1207, 1208
Bse118I RCCGGY 2 cut(s) 127, 340
Bse1I ACTGG 3 cut(s) 479, 869, 880
Bse3DI GCAATG 2 cut(s) 204, 672
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 1182
BseCI ATCGAT 1 cut(s) 568
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 7 cut(s) 424, 484, 522, 660, 919, 1190, 1204
BseLI CCNNNNNNNGG 7 cut(s) 601, 797, 798, 875, 1028, 1207, 1208
BseMI GCAATG 2 cut(s) 204, 672
BseMII CTCAG 2 cut(s) 600, 690
BseNI ACTGG 3 cut(s) 479, 869, 880
BseSI GKGCMC 1 cut(s) 1202
BseXI GCAGC 3 cut(s) 626, 985, 988
BsgI GTGCAG 1 cut(s) 371
Bsh1236I CGCG 1 cut(s) 897
BshFI GGCC 4 cut(s) 273, 879, 918, 1148
BshNI GGYRCC 2 cut(s) 174, 547
BshVI ATCGAT 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 758
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 5 cut(s) 128, 140, 274, 341, 1208
BslI CCNNNNNNNGG 7 cut(s) 601, 797, 798, 875, 1028, 1207, 1208
BsmAI GTCTC 3 cut(s) 92, 178, 589
BsmBI CGTCTC 1 cut(s) 92
BsnI GGCC 4 cut(s) 273, 879, 918, 1148
Bso31I GGTCTC 2 cut(s) 178, 589
BsoBI CYCGRG 1 cut(s) 741
Bsp1286I GDGCHC 3 cut(s) 31, 758, 1202
Bsp13I TCCGGA 1 cut(s) 139
Bsp1407I TGTACA 2 cut(s) 812, 1097
Bsp143I GATC 5 cut(s) 51, 111, 574, 964, 1087
BspACI CCGC 6 cut(s) 223, 230, 299, 401, 880, 1050
BspANI GGCC 4 cut(s) 273, 879, 918, 1148
BspCNI CTCAG 2 cut(s) 599, 691
BspDI ATCGAT 1 cut(s) 568
BspEI TCCGGA 1 cut(s) 139
BspFNI CGCG 1 cut(s) 897
BspHI TCATGA 1 cut(s) 1138
BspLI GGNNCC 2 cut(s) 176, 549
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 2 cut(s) 46, 1082
BspT107I GGYRCC 2 cut(s) 174, 547
BspTNI GGTCTC 2 cut(s) 178, 589
BsrDI GCAATG 2 cut(s) 204, 672
BsrFI RCCGGY 2 cut(s) 127, 340
BsrGI TGTACA 2 cut(s) 812, 1097
BsrI ACTGG 3 cut(s) 479, 869, 880
BssAI RCCGGY 2 cut(s) 127, 340
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 5 cut(s) 51, 111, 574, 964, 1087
BssNI GRCGYC 2 cut(s) 11, 175
Bst2UI CCWGG 1 cut(s) 1182
Bst4CI ACNGT 4 cut(s) 363, 514, 669, 1163
Bst6I CTCTTC 3 cut(s) 77, 90, 1137
BstACI GRCGYC 2 cut(s) 11, 175
BstAPI GCANNNNNTGC 1 cut(s) 337
BstAUI TGTACA 2 cut(s) 812, 1097
BstC8I GCNNGC 2 cut(s) 129, 916
BstDEI CTNAG 3 cut(s) 586, 699, 1223
BstEII GGTNACC 1 cut(s) 451
BstF5I GGATG 7 cut(s) 424, 484, 522, 660, 919, 1190, 1204
BstFNI CGCG 1 cut(s) 897
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 177, 614
BstKTI GATC 5 cut(s) 54, 114, 577, 967, 1090
BstMAI GTCTC 3 cut(s) 92, 178, 589
BstMBI GATC 5 cut(s) 51, 111, 574, 964, 1087
BstMWI GCNNNNNNNGC 6 cut(s) 26, 337, 400, 611, 620, 995
BstNI CCWGG 1 cut(s) 1182
BstNSI RCATGY 3 cut(s) 914, 940, 959
BstPI GGTNACC 1 cut(s) 451
BstSCI CCNGG 2 cut(s) 1180, 1207
BstSFI CTRYAG 1 cut(s) 527
BstSLI GKGCMC 1 cut(s) 1202
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 3 cut(s) 626, 985, 988
BstV2I GAAGAC 2 cut(s) 242, 809
Bsu15I ATCGAT 1 cut(s) 568
BsuI GTATCC 2 cut(s) 309, 1121
BsuRI GGCC 4 cut(s) 273, 879, 918, 1148
BsuTUI ATCGAT 1 cut(s) 568
BtsCI GGATG 7 cut(s) 424, 484, 522, 660, 919, 1190, 1204
BtsIMutI CAGTG 5 cut(s) 255, 486, 510, 873, 1159
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 129, 916
CaiI CAGNNNCTG 2 cut(s) 875, 1028
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 2 cut(s) 177, 614
Cfr10I RCCGGY 2 cut(s) 127, 340
Cfr13I GGNCC 1 cut(s) 1178
ClaI ATCGAT 1 cut(s) 568
CseI GACGC 2 cut(s) 9, 886
Csp6I GTAC 4 cut(s) 670, 813, 834, 1098
CviQI GTAC 4 cut(s) 670, 813, 834, 1098
DdeI CTNAG 3 cut(s) 586, 699, 1223
DinI GGCGCC 1 cut(s) 176
DpnI GATC 5 cut(s) 53, 113, 576, 966, 1089
DpnII GATC 5 cut(s) 51, 111, 574, 964, 1087
EaeI YGGCCR 1 cut(s) 877
Eam1104I CTCTTC 3 cut(s) 77, 90, 1137
EarI CTCTTC 3 cut(s) 77, 90, 1137
Ecl136II GAGCTC 1 cut(s) 756
Eco147I AGGCCT 1 cut(s) 1148
Eco24I GRGCYC 2 cut(s) 31, 758
Eco31I GGTCTC 2 cut(s) 178, 589
Eco47I GGWCC 1 cut(s) 1178
Eco53kI GAGCTC 1 cut(s) 756
Eco57I CTGAAG 2 cut(s) 996, 1063
Eco88I CYCGRG 1 cut(s) 741
Eco91I GGTNACC 1 cut(s) 451
EcoICRI GAGCTC 1 cut(s) 756
EcoO65I GGTNACC 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 843
EcoRII CCWGG 1 cut(s) 1180
EcoT38I GRGCYC 2 cut(s) 31, 758
EgeI GGCGCC 1 cut(s) 176
EheI GGCGCC 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 92
FauI CCCGC 1 cut(s) 223
Fnu4HI GCNGC 4 cut(s) 615, 880, 999, 1002
FokI GGATG 7 cut(s) 431, 471, 509, 667, 906, 1191, 1197
FriOI GRGCYC 2 cut(s) 31, 758
Fsp4HI GCNGC 4 cut(s) 615, 880, 999, 1002
FspBI CTAG 4 cut(s) 24, 606, 825, 1228
GlaI GCGC 2 cut(s) 176, 613
GluI GCNGC 4 cut(s) 615, 880, 999, 1002
GsuI CTGGAG 1 cut(s) 1005
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 4 cut(s) 273, 879, 918, 1148
HapII CCGG 5 cut(s) 128, 140, 274, 341, 1208
HgaI GACGC 2 cut(s) 9, 886
HhaI GCGC 2 cut(s) 177, 614
Hin1I GRCGYC 2 cut(s) 11, 175
Hin6I GCGC 2 cut(s) 175, 612
HinP1I GCGC 2 cut(s) 175, 612
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 7 cut(s) 277, 562, 591, 681, 773, 828, 1064
HpaII CCGG 5 cut(s) 128, 140, 274, 341, 1208
HphI GGTGA 4 cut(s) 445, 782, 794, 1082
Hpy166II GTNNAC 6 cut(s) 247, 255, 464, 822, 866, 1098
Hpy188I TCNGA 5 cut(s) 51, 63, 561, 849, 891
Hpy188III TCNNGA 9 cut(s) 140, 281, 431, 588, 698, 743, 1010, 1139, 1176
Hpy8I GTNNAC 6 cut(s) 247, 255, 464, 822, 866, 1098
Hpy99I CGWCG 3 cut(s) 13, 16, 367
HpyAV CCTTC 5 cut(s) 358, 697, 740, 744, 971
HpyCH4III ACNGT 4 cut(s) 363, 514, 669, 1163
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 8 cut(s) 34, 42, 388, 470, 484, 677, 907, 914
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 337, 400, 611, 620, 995
HpyF3I CTNAG 3 cut(s) 586, 699, 1223
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 2 cut(s) 11, 175
HspAI GCGC 2 cut(s) 175, 612
KasI GGCGCC 1 cut(s) 174
Kpn2I TCCGGA 1 cut(s) 139
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Kzo9I GATC 5 cut(s) 51, 111, 574, 964, 1087
LmnI GCTCC 4 cut(s) 706, 753, 793, 1123
Lsp1109I GCAGC 3 cut(s) 626, 985, 988
LweI GCATC 6 cut(s) 479, 493, 531, 709, 775, 1226
MaeI CTAG 4 cut(s) 24, 606, 825, 1228
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 3 cut(s) 281, 451, 871
MalI GATC 5 cut(s) 53, 113, 576, 966, 1089
MboI GATC 5 cut(s) 51, 111, 574, 964, 1087
MboII GAAGA 7 cut(s) 94, 100, 107, 247, 814, 1073, 1154
MhlI GDGCHC 3 cut(s) 31, 758, 1202
MluCI AATT 4 cut(s) 36, 426, 843, 1102
MluI ACGCGT 1 cut(s) 895
Mly113I GGCGCC 1 cut(s) 175
MlyI GAGTC 2 cut(s) 585, 690
MreI CGCCGGCG 1 cut(s) 127
MroI TCCGGA 1 cut(s) 139
MroNI GCCGGC 1 cut(s) 127
MseI TTAA 3 cut(s) 216, 303, 357
MslI CAYNNNNRTG 3 cut(s) 393, 489, 960
MspA1I CMGCKG 1 cut(s) 974
MspI CCGG 5 cut(s) 128, 140, 274, 341, 1208
MspR9I CCNGG 2 cut(s) 1182, 1209
MvaI CCWGG 1 cut(s) 1182
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 6 cut(s) 26, 337, 400, 611, 620, 995
NaeI GCCGGC 1 cut(s) 129
NarI GGCGCC 1 cut(s) 175
NciI CCSGG 1 cut(s) 1209
NdeII GATC 5 cut(s) 51, 111, 574, 964, 1087
NgoMIV GCCGGC 1 cut(s) 127
NlaIV GGNNCC 2 cut(s) 176, 549
NmuCI GTSAC 3 cut(s) 281, 451, 871
NspI RCATGY 3 cut(s) 914, 940, 959
PaeR7I CTCGAG 1 cut(s) 741
PagI TCATGA 1 cut(s) 1138
PceI AGGCCT 1 cut(s) 1148
PciI ACATGT 1 cut(s) 955
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 574
PdiI GCCGGC 1 cut(s) 129
PfeI GAWTC 5 cut(s) 277, 562, 773, 828, 1064
PflMI CCANNNNNTGG 2 cut(s) 875, 1028
PkrI GCNGC 4 cut(s) 616, 881, 1000, 1003
PleI GAGTC 2 cut(s) 585, 689
PluTI GGCGCC 1 cut(s) 178
PpsI GAGTC 2 cut(s) 585, 689
PscI ACATGT 1 cut(s) 955
Psp124BI GAGCTC 1 cut(s) 758
Psp6I CCWGG 1 cut(s) 1180
PspEI GGTNACC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 1180
PspN4I GGNNCC 2 cut(s) 176, 549
PspPI GGNCC 1 cut(s) 1178
PstNI CAGNNNCTG 2 cut(s) 875, 1028
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 4 cut(s) 671, 814, 835, 1099
RsaNI GTAC 4 cut(s) 670, 813, 834, 1098
RseI CAYNNNNRTG 3 cut(s) 393, 489, 960
SacI GAGCTC 1 cut(s) 758
SaqAI TTAA 3 cut(s) 216, 303, 357
SatI GCNGC 4 cut(s) 615, 880, 999, 1002
Sau3AI GATC 5 cut(s) 51, 111, 574, 964, 1087
Sau96I GGNCC 1 cut(s) 1178
SchI GAGTC 2 cut(s) 585, 690
ScrFI CCNGG 2 cut(s) 1182, 1209
SduI GDGCHC 3 cut(s) 31, 758, 1202
SfaNI GCATC 6 cut(s) 479, 493, 531, 709, 775, 1226
SfcI CTRYAG 1 cut(s) 527
SfoI GGCGCC 1 cut(s) 176
Sfr274I CTCGAG 1 cut(s) 741
SgrAI CRCCGGYG 1 cut(s) 127
SinI GGWCC 1 cut(s) 1178
SlaI CTCGAG 1 cut(s) 741
SmiMI CAYNNNNRTG 3 cut(s) 393, 489, 960
SmlI CTYRAG 1 cut(s) 741
SmoI CTYRAG 1 cut(s) 741
Sse9I AATT 4 cut(s) 36, 426, 843, 1102
SseBI AGGCCT 1 cut(s) 1148
SsiI CCGC 6 cut(s) 223, 230, 299, 401, 880, 1050
SspDI GGCGCC 1 cut(s) 174
SspMI CTAG 4 cut(s) 24, 606, 825, 1228
SstI GAGCTC 1 cut(s) 758
StuI AGGCCT 1 cut(s) 1148
StyD4I CCNGG 2 cut(s) 1180, 1207
TaaI ACNGT 4 cut(s) 363, 514, 669, 1163
TaiI ACGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 568, 742
TasI AATT 4 cut(s) 36, 426, 843, 1102
TatI WGTACW 3 cut(s) 669, 812, 1097
TauI GCSGC 1 cut(s) 882
TfiI GAWTC 5 cut(s) 277, 562, 773, 828, 1064
Tru1I TTAA 3 cut(s) 216, 303, 357
Tru9I TTAA 3 cut(s) 216, 303, 357
TscAI CASTG 5 cut(s) 262, 486, 517, 880, 1166
TseFI GTSAC 3 cut(s) 281, 451, 871
TseI GCWGC 3 cut(s) 614, 998, 1001
Tsp45I GTSAC 3 cut(s) 281, 451, 871
TspDTI ATGAA 8 cut(s) 95, 193, 340, 411, 554, 650, 1074, 1155
TspGWI ACGGA 2 cut(s) 116, 160
TspRI CASTG 5 cut(s) 262, 486, 517, 880, 1166
Van91I CCANNNNNTGG 2 cut(s) 875, 1028
VpaK11BI GGWCC 1 cut(s) 1178
XapI RAATTY 3 cut(s) 36, 426, 843
XceI RCATGY 3 cut(s) 914, 940, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 1209
XhoI CTCGAG 1 cut(s) 741
XspI CTAG 4 cut(s) 24, 606, 825, 1228
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.