Rh7BG105000

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009164
Physical Location & Seq
Reverse (-)
7857648 .. 7860835
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG105000.1

Sequence Viewer

Length: 1125 bp
ATGGAAATCGTCTGGCAATCGCCGGCGAACCCTCCGGAACGGAAAGATTACATCTTTCGCAACGGGAGGCGCCATGTGAGACCCTACTACTTTGAGTTCATTGCTCATGTTAAGAACCGGTGGGCGGGGAAGACCATCGTTGACTTGTTCACTGACGAGTTCAAAGGCCGGAATCGTGACTACTATATTAGTGCGGTTAAATGTGGGAGGATACAAGTTGATGGGCAACTTGTGCCGGTTTCCTTCATAGTTAAACCGTCGCAAAAGATAAGCCATTTCCTGCACAGGCATGAACCGCCCGTGATGACTTGGGATGTGAAAATTCTTGAAACAGAACCCGATGTGGTCACCATTTGTAAACCTGCATCTGTTCCAGTGCATCCATGTGGTCAGTATCGTAAGAACACTGTTGTTGGCATCCTACAGGCAGAGCATGGCTTGGCACCTCTATTTCCGATTCATCGACTAGATCGTCTGGTCTCAGGACTCCTTATCTTGGCTAGAAGCGCAGCACAAGCTGACATTTTCAGGCAACAGATTGAGGGAGGGATGGTTCATAAACAGTACATTGCAAGAGTCATTGGAGAGTTTCCTGAGGAGGAGCAAGTTGTAGATGCTAACATAAATCATAATGCTCGAGAAGGAAGGAGCTCTGCCGAGGTCGGTGATTCTTGTGGTGATGCTCCCACAAAGGGGAAGACTGCTTGTACAAAGTTTACTAGAATCAGTACGAATGGAATTCAGAGCATTGTCTTGTGTGAACCAATCACTGGCCGCACTCATCAAATACGCGTCCATTTGCAACATGCAGGCCATCCAATAGCGAATGACATGCTTTACCTGTCCAAACATGTAAGTGATCGTTCAGCTGAAGGAATGGGTGCTGATAGAGCTGCTGCTAATCCAGACCACTCTCCAGCTTCTGGTGTTGGCGAAAATAAATCCACTTCGCATGAAGATTCTGGTGAGGACTTCAGCATTGATCCAATGTGTACAAATTGTCCAAGTTTAGCTCCAAAAGGATACGATTATCATGAAGAGGCCTTGTGGCTACACTGTTTTAGATATTCTGGACCCGGATGGGTTTATGAATGCCCACATCCGGATTGGGCATCACTTAGCTAG

Protein Analysis

374

Amino Acids

41.86

Weight (kDa)

6.7

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 114 - 269 1.8e-28 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 471
Acc36I ACCTGC 1 cut(s) 370
AccB1I GGYRCC 2 cut(s) 69, 442
AccB7I CCANNNNNTGG 2 cut(s) 770, 923
AccII CGCG 1 cut(s) 792
AccIII TCCGGA 2 cut(s) 34, 1102
AciI CCGC 4 cut(s) 125, 194, 296, 775
AclWI GGATC 1 cut(s) 977
AcoI YGGCCR 1 cut(s) 772
AcsI RAATTY 2 cut(s) 321, 738
AcuI CTGAAG 2 cut(s) 891, 958
AcyI GRCGYC 1 cut(s) 70
AfaI GTAC 4 cut(s) 566, 709, 730, 994
AfiI CCNNNNNNNGG 7 cut(s) 124, 496, 692, 693, 770, 923, 1102
AflIII ACRYGT 2 cut(s) 790, 850
AgeI ACCGGT 1 cut(s) 117
AgsI TTSAA 2 cut(s) 163, 329
AloI GAACNNNNNNTCC 2 cut(s) 537, 569
AluBI AGCT 7 cut(s) 518, 651, 869, 893, 920, 1013, 1122
AluI AGCT 7 cut(s) 518, 651, 869, 893, 920, 1013, 1122
Alw21I GWGCWC 1 cut(s) 653
Alw26I GTCTC 2 cut(s) 73, 484
AlwI GGATC 1 cut(s) 977
AlwNI CAGNNNCTG 1 cut(s) 923
Ama87I CYCGRG 1 cut(s) 636
Aor13HI TCCGGA 2 cut(s) 34, 1102
AoxI GGCC 4 cut(s) 166, 772, 811, 1041
ApeKI GCWGC 3 cut(s) 509, 893, 896
ApoI RAATTY 2 cut(s) 321, 738
AsiGI ACCGGT 1 cut(s) 117
AspLEI GCGC 2 cut(s) 72, 509
AspS9I GGNCC 1 cut(s) 1073
AsuC2I CCSGG 1 cut(s) 1077
AsuHPI GGTGA 4 cut(s) 340, 677, 689, 977
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 1 cut(s) 1073
AxyI CCTNAGG 1 cut(s) 594
BanI GGYRCC 2 cut(s) 69, 442
BanII GRGCYC 1 cut(s) 653
BbsI GAAGAC 2 cut(s) 137, 704
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 3 cut(s) 521, 880, 883
BccI CCATC 5 cut(s) 143, 215, 544, 822, 1074
BcgI CGANNNNNNTGC 2 cut(s) 814, 848
BciVI GTATCC 2 cut(s) 204, 1016
BcnI CCSGG 1 cut(s) 1077
BcoDI GTCTC 2 cut(s) 73, 484
BfaI CTAG 4 cut(s) 467, 501, 720, 1123
BfmI CTRYAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 370
BfuI GTATCC 2 cut(s) 204, 1016
BisI GCNGC 4 cut(s) 510, 775, 894, 897
BlsI GCNGC 4 cut(s) 511, 776, 895, 898
Bme1390I CCNGG 1 cut(s) 1077
Bme18I GGWCC 1 cut(s) 1073
BmeT110I CYCGRG 1 cut(s) 636
BmgT120I GGNCC 1 cut(s) 1073
BmiI GGNNCC 3 cut(s) 71, 444, 1075
BmrFI CCNGG 1 cut(s) 1077
BmsI GCATC 6 cut(s) 374, 388, 426, 604, 670, 1121
BpiI GAAGAC 2 cut(s) 137, 704
BpmI CTGGAG 1 cut(s) 900
BpuMI CCSGG 1 cut(s) 1077
BsaHI GRCGYC 1 cut(s) 70
BsaI GGTCTC 2 cut(s) 73, 484
BsaJI CCNNGG 1 cut(s) 657
BsaWI WCCGGW 3 cut(s) 34, 117, 1102
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 7 cut(s) 124, 496, 692, 693, 770, 923, 1102
Bse118I RCCGGY 3 cut(s) 22, 117, 235
Bse1I ACTGG 2 cut(s) 374, 775
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 2 cut(s) 99, 567
BseAI TCCGGA 2 cut(s) 34, 1102
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 7 cut(s) 319, 379, 417, 555, 814, 1085, 1099
BseLI CCNNNNNNNGG 7 cut(s) 124, 496, 692, 693, 770, 923, 1102
BseMI GCAATG 2 cut(s) 99, 567
BseMII CTCAG 2 cut(s) 495, 585
BseNI ACTGG 2 cut(s) 374, 775
BseRI GAGGAG 2 cut(s) 611, 614
BseXI GCAGC 3 cut(s) 521, 880, 883
BsgI GTGCAG 1 cut(s) 266
Bsh1236I CGCG 1 cut(s) 792
BshFI GGCC 4 cut(s) 168, 774, 813, 1043
BshNI GGYRCC 2 cut(s) 69, 442
BshTI ACCGGT 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 653
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 7 cut(s) 23, 35, 118, 169, 236, 1077, 1103
BslI CCNNNNNNNGG 7 cut(s) 124, 496, 692, 693, 770, 923, 1102
BsmAI GTCTC 2 cut(s) 73, 484
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 4 cut(s) 168, 774, 813, 1043
Bso31I GGTCTC 2 cut(s) 73, 484
BsoBI CYCGRG 1 cut(s) 636
Bsp1286I GDGCHC 1 cut(s) 653
Bsp13I TCCGGA 2 cut(s) 34, 1102
Bsp1407I TGTACA 2 cut(s) 707, 992
Bsp143I GATC 3 cut(s) 469, 859, 982
BspACI CCGC 4 cut(s) 125, 194, 296, 775
BspANI GGCC 4 cut(s) 168, 774, 813, 1043
BspCNI CTCAG 2 cut(s) 494, 586
BspEI TCCGGA 2 cut(s) 34, 1102
BspFNI CGCG 1 cut(s) 792
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 3 cut(s) 71, 444, 1075
BspMI ACCTGC 1 cut(s) 370
BspPI GGATC 1 cut(s) 977
BspT107I GGYRCC 2 cut(s) 69, 442
BspTNI GGTCTC 2 cut(s) 73, 484
BsrDI GCAATG 2 cut(s) 99, 567
BsrFI RCCGGY 3 cut(s) 22, 117, 235
BsrGI TGTACA 2 cut(s) 707, 992
BsrI ACTGG 2 cut(s) 374, 775
BssAI RCCGGY 3 cut(s) 22, 117, 235
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 3 cut(s) 469, 859, 982
BssNI GRCGYC 1 cut(s) 70
Bst4CI ACNGT 4 cut(s) 258, 409, 564, 1058
Bst6I CTCTTC 1 cut(s) 1032
BstACI GRCGYC 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 232
BstAUI TGTACA 2 cut(s) 707, 992
BstC8I GCNNGC 2 cut(s) 24, 811
BstDEI CTNAG 3 cut(s) 481, 594, 1118
BstEII GGTNACC 1 cut(s) 346
BstF5I GGATG 7 cut(s) 319, 379, 417, 555, 814, 1085, 1099
BstFNI CGCG 1 cut(s) 792
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 2 cut(s) 72, 509
BstKTI GATC 3 cut(s) 472, 862, 985
BstMAI GTCTC 2 cut(s) 73, 484
BstMBI GATC 3 cut(s) 469, 859, 982
BstMWI GCNNNNNNNGC 5 cut(s) 232, 295, 506, 515, 890
BstNSI RCATGY 3 cut(s) 809, 835, 854
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 1075
BstSFI CTRYAG 1 cut(s) 422
BstUI CGCG 1 cut(s) 792
BstV1I GCAGC 3 cut(s) 521, 880, 883
BstV2I GAAGAC 2 cut(s) 137, 704
Bsu36I CCTNAGG 1 cut(s) 594
BsuI GTATCC 2 cut(s) 204, 1016
BsuRI GGCC 4 cut(s) 168, 774, 813, 1043
BtsCI GGATG 7 cut(s) 319, 379, 417, 555, 814, 1085, 1099
BtsIMutI CAGTG 5 cut(s) 150, 381, 405, 768, 1054
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 2 cut(s) 24, 811
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 2 cut(s) 72, 509
Cfr10I RCCGGY 3 cut(s) 22, 117, 235
Cfr13I GGNCC 1 cut(s) 1073
CseI GACGC 1 cut(s) 781
Csp6I GTAC 4 cut(s) 565, 708, 729, 993
CspAI ACCGGT 1 cut(s) 117
CviQI GTAC 4 cut(s) 565, 708, 729, 993
DdeI CTNAG 3 cut(s) 481, 594, 1118
DinI GGCGCC 1 cut(s) 71
DpnI GATC 3 cut(s) 471, 861, 984
DpnII GATC 3 cut(s) 469, 859, 982
DrdI GACNNNNNNGTC 1 cut(s) 471
DseDI GACNNNNNNGTC 1 cut(s) 471
EaeI YGGCCR 1 cut(s) 772
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
Ecl136II GAGCTC 1 cut(s) 651
Eco147I AGGCCT 1 cut(s) 1043
Eco24I GRGCYC 1 cut(s) 653
Eco31I GGTCTC 2 cut(s) 73, 484
Eco47I GGWCC 1 cut(s) 1073
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 2 cut(s) 891, 958
Eco81I CCTNAGG 1 cut(s) 594
Eco88I CYCGRG 1 cut(s) 636
Eco91I GGTNACC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 651
EcoO65I GGTNACC 1 cut(s) 346
EcoRI GAATTC 1 cut(s) 738
EcoT38I GRGCYC 1 cut(s) 653
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
FauI CCCGC 1 cut(s) 118
Fnu4HI GCNGC 4 cut(s) 510, 775, 894, 897
FokI GGATG 7 cut(s) 326, 366, 404, 562, 801, 1086, 1092
FriOI GRGCYC 1 cut(s) 653
Fsp4HI GCNGC 4 cut(s) 510, 775, 894, 897
FspBI CTAG 4 cut(s) 467, 501, 720, 1123
GlaI GCGC 2 cut(s) 71, 508
GluI GCNGC 4 cut(s) 510, 775, 894, 897
GsuI CTGGAG 1 cut(s) 900
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 4 cut(s) 168, 774, 813, 1043
HapII CCGG 7 cut(s) 23, 35, 118, 169, 236, 1077, 1103
HgaI GACGC 1 cut(s) 781
HhaI GCGC 2 cut(s) 72, 509
Hin1I GRCGYC 1 cut(s) 70
Hin6I GCGC 2 cut(s) 70, 507
HinP1I GCGC 2 cut(s) 70, 507
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 7 cut(s) 172, 457, 486, 576, 668, 723, 959
HpaII CCGG 7 cut(s) 23, 35, 118, 169, 236, 1077, 1103
HphI GGTGA 4 cut(s) 340, 677, 689, 977
Hpy166II GTNNAC 6 cut(s) 142, 150, 359, 717, 761, 993
Hpy188I TCNGA 2 cut(s) 456, 744
Hpy8I GTNNAC 6 cut(s) 142, 150, 359, 717, 761, 993
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 4 cut(s) 253, 635, 639, 866
HpyCH4III ACNGT 4 cut(s) 258, 409, 564, 1058
HpyCH4V TGCA 6 cut(s) 283, 365, 379, 572, 802, 809
HpyF10VI GCNNNNNNNGC 5 cut(s) 232, 295, 506, 515, 890
HpyF3I CTNAG 3 cut(s) 481, 594, 1118
Hsp92I GRCGYC 1 cut(s) 70
HspAI GCGC 2 cut(s) 70, 507
KasI GGCGCC 1 cut(s) 69
Kpn2I TCCGGA 2 cut(s) 34, 1102
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Kzo9I GATC 3 cut(s) 469, 859, 982
LmnI GCTCC 4 cut(s) 601, 648, 688, 1018
Lsp1109I GCAGC 3 cut(s) 521, 880, 883
LweI GCATC 6 cut(s) 374, 388, 426, 604, 670, 1121
MaeI CTAG 4 cut(s) 467, 501, 720, 1123
MaeIII GTNAC 2 cut(s) 176, 346
MalI GATC 3 cut(s) 471, 861, 984
MboI GATC 3 cut(s) 469, 859, 982
MboII GAAGA 4 cut(s) 142, 709, 968, 1049
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 3 cut(s) 321, 738, 997
MluI ACGCGT 1 cut(s) 790
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 2 cut(s) 480, 585
MreI CGCCGGCG 1 cut(s) 22
MroI TCCGGA 2 cut(s) 34, 1102
MroNI GCCGGC 1 cut(s) 22
MseI TTAA 3 cut(s) 111, 198, 252
MslI CAYNNNNRTG 3 cut(s) 288, 384, 855
MspA1I CMGCKG 1 cut(s) 869
MspI CCGG 7 cut(s) 23, 35, 118, 169, 236, 1077, 1103
MspR9I CCNGG 1 cut(s) 1077
Mva1269I GAATGC 1 cut(s) 1097
MvnI CGCG 1 cut(s) 792
MwoI GCNNNNNNNGC 5 cut(s) 232, 295, 506, 515, 890
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 70
NciI CCSGG 1 cut(s) 1077
NdeII GATC 3 cut(s) 469, 859, 982
NgoMIV GCCGGC 1 cut(s) 22
NlaIV GGNNCC 3 cut(s) 71, 444, 1075
NmeAIII GCCGAG 1 cut(s) 682
NmuCI GTSAC 2 cut(s) 176, 346
NspI RCATGY 3 cut(s) 809, 835, 854
PaeR7I CTCGAG 1 cut(s) 636
PagI TCATGA 1 cut(s) 1033
PceI AGGCCT 1 cut(s) 1043
PciI ACATGT 1 cut(s) 850
PcsI WCGNNNNNNNCGW 1 cut(s) 469
PctI GAATGC 1 cut(s) 1097
PdiI GCCGGC 1 cut(s) 24
PfeI GAWTC 5 cut(s) 172, 457, 668, 723, 959
PflMI CCANNNNNTGG 2 cut(s) 770, 923
PinAI ACCGGT 1 cut(s) 117
PkrI GCNGC 4 cut(s) 511, 776, 895, 898
PleI GAGTC 2 cut(s) 480, 584
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 2 cut(s) 480, 584
PscI ACATGT 1 cut(s) 850
Psp124BI GAGCTC 1 cut(s) 653
PspEI GGTNACC 1 cut(s) 346
PspN4I GGNNCC 3 cut(s) 71, 444, 1075
PspPI GGNCC 1 cut(s) 1073
PstNI CAGNNNCTG 1 cut(s) 923
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 4 cut(s) 566, 709, 730, 994
RsaNI GTAC 4 cut(s) 565, 708, 729, 993
RseI CAYNNNNRTG 3 cut(s) 288, 384, 855
SacI GAGCTC 1 cut(s) 653
SaqAI TTAA 3 cut(s) 111, 198, 252
SatI GCNGC 4 cut(s) 510, 775, 894, 897
Sau3AI GATC 3 cut(s) 469, 859, 982
Sau96I GGNCC 1 cut(s) 1073
SchI GAGTC 2 cut(s) 480, 585
ScrFI CCNGG 1 cut(s) 1077
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 6 cut(s) 374, 388, 426, 604, 670, 1121
SfcI CTRYAG 1 cut(s) 422
SfoI GGCGCC 1 cut(s) 71
Sfr274I CTCGAG 1 cut(s) 636
SgrAI CRCCGGYG 1 cut(s) 22
SinI GGWCC 1 cut(s) 1073
SlaI CTCGAG 1 cut(s) 636
SmiMI CAYNNNNRTG 3 cut(s) 288, 384, 855
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
Sse9I AATT 3 cut(s) 321, 738, 997
SseBI AGGCCT 1 cut(s) 1043
SsiI CCGC 4 cut(s) 125, 194, 296, 775
SspDI GGCGCC 1 cut(s) 69
SspMI CTAG 4 cut(s) 467, 501, 720, 1123
SstI GAGCTC 1 cut(s) 653
StuI AGGCCT 1 cut(s) 1043
StyD4I CCNGG 1 cut(s) 1075
TaaI ACNGT 4 cut(s) 258, 409, 564, 1058
TaqI TCGA 2 cut(s) 463, 637
TasI AATT 3 cut(s) 321, 738, 997
TatI WGTACW 3 cut(s) 564, 707, 992
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 5 cut(s) 172, 457, 668, 723, 959
Tru1I TTAA 3 cut(s) 111, 198, 252
Tru9I TTAA 3 cut(s) 111, 198, 252
TscAI CASTG 5 cut(s) 157, 381, 412, 775, 1061
TseFI GTSAC 2 cut(s) 176, 346
TseI GCWGC 3 cut(s) 509, 893, 896
Tsp45I GTSAC 2 cut(s) 176, 346
TspDTI ATGAA 8 cut(s) 88, 235, 306, 449, 545, 969, 1050, 1104
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 5 cut(s) 157, 381, 412, 775, 1061
Van91I CCANNNNNTGG 2 cut(s) 770, 923
VpaK11BI GGWCC 1 cut(s) 1073
XapI RAATTY 2 cut(s) 321, 738
XceI RCATGY 3 cut(s) 809, 835, 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 1104
XhoI CTCGAG 1 cut(s) 636
XspI CTAG 4 cut(s) 467, 501, 720, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.