RchiOBHm_Chr7g0189401

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009164
Physical Location & Seq
Reverse (-)
8709916 .. 8712625
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16914

Sequence Viewer

Length: 921 bp
ATGATAAGTTTGAGTGCGGTTAAATGTGGGAGGATACAAGTTGATGGGCAACTTGTGCCGGTTTCCTTCATAGTTAAACCGTCGCAAAAGATAAGCCACTTCCTGCACAGGCATGAACCGCCCGTGATGACTTGGGATGTGAAAATTCTTGAAACAGAACCCGATGTGGTCACCATTTGTAAACCTGCATCTGTTCCAGTGCATCCATGTGGTCAGTATCGTAAGAACACTGTTGTTGGCATCCTACAGGCAGAGCATGGCTTGGCACCTCTATTTCGTATCCAAAATTTAAGACATAAAACTTCTGGAAGTTTTTTTTTTTTTTTTGAGAGTTCAGTTAATGTTATTGAGGGAGGGATGGTTCATAAACAGTACATTGCAAGAGTGATTGGAGAGTTTCCTGAGGAGGAGCAAGTTGTAGATGCTAACATAAATCATAATGCTCGAGAAGGAAGGAGCTCTGCCGAGGTCGGTGATTCTTGTGGTGATGCTCCCACAAAGGGGAAGACTGCTTGTACAAAGTTTACTAGAATCAGTACGAATGGAACTCAGAGCATTGTAATGTGTGAATCGGTCACTGGCTGCACTCATAAAATATGCGTCCATTTGCAACATGCAGGCCATCCAATAGCGAATGACATGCTTTACCTGTCCAAACATGTAAGTGATCGTTCAGCTGAAGGAATAGGTGCTGATAGAGCTGCTGCTAATCCAGACCACTCTCCGGCTTCTGGTGTTGGCGAAAATAAATCCACTTCGCATGAAGATTCTAGTGAGGACTTCAGCATTGATCCAATGTGTTCAAATTGTCCAAATTTAGCTCCAAAAGGATATTCTGTACCCGGATGGGTTTATGAATGCCCACATCCGGATTGGCATCACTTAGCTAGTTATTCAAACAAATCTGTATTTCTTTATTAA

Protein Analysis

306

Amino Acids

33.47

Weight (kDa)

6.4

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 55 - 205 7.2e-08 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 265
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 2 cut(s) 17, 119
AclWI GGATC 1 cut(s) 785
AcsI RAATTY 3 cut(s) 144, 286, 814
AcuI CTGAAG 2 cut(s) 699, 766
AfaI GTAC 4 cut(s) 374, 517, 538, 840
AfiI CCNNNNNNNGG 5 cut(s) 500, 501, 724, 731, 868
AflIII ACRYGT 1 cut(s) 658
AgsI TTSAA 3 cut(s) 152, 804, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 5 cut(s) 459, 677, 701, 821, 887
AluI AGCT 5 cut(s) 459, 677, 701, 821, 887
Alw21I GWGCWC 1 cut(s) 461
AlwI GGATC 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 444
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 1 cut(s) 619
ApeKI GCWGC 3 cut(s) 582, 701, 704
ApoI RAATTY 3 cut(s) 144, 286, 814
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 3 cut(s) 163, 485, 497
AvaI CYCGRG 1 cut(s) 444
AxyI CCTNAGG 1 cut(s) 402
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 3 cut(s) 569, 688, 691
BccI CCATC 4 cut(s) 38, 352, 630, 840
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciVI GTATCC 2 cut(s) 27, 290
BcnI CCSGG 1 cut(s) 843
BfaI CTAG 3 cut(s) 528, 771, 888
BfmI CTRYAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 193
BfuI GTATCC 2 cut(s) 27, 290
BisI GCNGC 3 cut(s) 583, 702, 705
BlsI GCNGC 3 cut(s) 584, 703, 706
Bme1390I CCNGG 1 cut(s) 843
BmeT110I CYCGRG 1 cut(s) 444
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 843
BmsI GCATC 6 cut(s) 197, 211, 249, 412, 478, 886
BpiI GAAGAC 1 cut(s) 512
BpuMI CCSGG 1 cut(s) 843
BsaBI GATNNNNATC 1 cut(s) 876
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 5 cut(s) 500, 501, 724, 731, 868
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 197, 583
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 876
BseAI TCCGGA 1 cut(s) 868
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 7 cut(s) 142, 202, 240, 363, 622, 851, 865
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 5 cut(s) 500, 501, 724, 731, 868
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 393, 563
BseNI ACTGG 2 cut(s) 197, 583
BseRI GAGGAG 2 cut(s) 419, 422
BseXI GCAGC 3 cut(s) 569, 688, 691
BsgI GTGCAG 2 cut(s) 89, 568
BshFI GGCC 1 cut(s) 621
BshNI GGYRCC 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 4 cut(s) 59, 725, 843, 869
BslI CCNNNNNNNGG 5 cut(s) 500, 501, 724, 731, 868
BsmI GAATGC 1 cut(s) 863
BsnI GGCC 1 cut(s) 621
BsoBI CYCGRG 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 461
Bsp13I TCCGGA 1 cut(s) 868
Bsp1407I TGTACA 1 cut(s) 515
Bsp143I GATC 2 cut(s) 667, 790
BspACI CCGC 2 cut(s) 17, 119
BspANI GGCC 1 cut(s) 621
BspCNI CTCAG 2 cut(s) 394, 562
BspEI TCCGGA 1 cut(s) 868
BspLI GGNNCC 1 cut(s) 267
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 265
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 515
BsrI ACTGG 2 cut(s) 197, 583
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 2 cut(s) 667, 790
Bst4CI ACNGT 3 cut(s) 81, 232, 372
BstAPI GCANNNNNTGC 1 cut(s) 55
BstAUI TGTACA 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 3 cut(s) 402, 549, 883
BstEII GGTNACC 1 cut(s) 169
BstF5I GGATG 7 cut(s) 142, 202, 240, 363, 622, 851, 865
BstKTI GATC 2 cut(s) 670, 793
BstMBI GATC 2 cut(s) 667, 790
BstMWI GCNNNNNNNGC 3 cut(s) 55, 118, 698
BstNSI RCATGY 3 cut(s) 617, 643, 662
BstPI GGTNACC 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 841
BstSFI CTRYAG 1 cut(s) 245
BstV1I GCAGC 3 cut(s) 569, 688, 691
BstV2I GAAGAC 1 cut(s) 512
Bsu36I CCTNAGG 1 cut(s) 402
BsuI GTATCC 2 cut(s) 27, 290
BsuRI GGCC 1 cut(s) 621
BtsCI GGATG 7 cut(s) 142, 202, 240, 363, 622, 851, 865
BtsIMutI CAGTG 3 cut(s) 204, 228, 576
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 619
Cfr10I RCCGGY 1 cut(s) 58
CseI GACGC 1 cut(s) 589
Csp6I GTAC 4 cut(s) 373, 516, 537, 839
CviAII CATG 7 cut(s) 113, 207, 257, 614, 640, 659, 761
CviQI GTAC 4 cut(s) 373, 516, 537, 839
DdeI CTNAG 3 cut(s) 402, 549, 883
DpnI GATC 2 cut(s) 669, 792
DpnII GATC 2 cut(s) 667, 790
Ecl136II GAGCTC 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 2 cut(s) 699, 766
Eco81I CCTNAGG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 444
Eco91I GGTNACC 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 459
EcoO65I GGTNACC 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 461
FaeI CATG 7 cut(s) 116, 210, 260, 617, 643, 662, 764
FatI CATG 7 cut(s) 112, 206, 256, 613, 639, 658, 760
Fnu4HI GCNGC 3 cut(s) 583, 702, 705
FokI GGATG 7 cut(s) 149, 189, 227, 370, 609, 852, 858
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 583, 702, 705
FspBI CTAG 3 cut(s) 528, 771, 888
GluI GCNGC 3 cut(s) 583, 702, 705
HaeIII GGCC 1 cut(s) 621
HapII CCGG 4 cut(s) 59, 725, 843, 869
HgaI GACGC 1 cut(s) 589
Hin1II CATG 7 cut(s) 116, 210, 260, 617, 643, 662, 764
HinfI GANTC 4 cut(s) 476, 531, 569, 767
HpaII CCGG 4 cut(s) 59, 725, 843, 869
HphI GGTGA 3 cut(s) 163, 485, 497
Hpy166II GTNNAC 2 cut(s) 182, 525
Hpy188I TCNGA 1 cut(s) 552
Hpy188III TCNNGA 6 cut(s) 149, 306, 401, 446, 713, 869
Hpy8I GTNNAC 2 cut(s) 182, 525
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 76, 443, 447, 674
HpyCH4III ACNGT 3 cut(s) 81, 232, 372
HpyCH4V TGCA 7 cut(s) 106, 188, 202, 380, 585, 610, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 118, 698
HpyF3I CTNAG 3 cut(s) 402, 549, 883
Hsp92II CATG 7 cut(s) 116, 210, 260, 617, 643, 662, 764
Kpn2I TCCGGA 1 cut(s) 868
Kzo9I GATC 2 cut(s) 667, 790
LmnI GCTCC 4 cut(s) 409, 456, 496, 826
Lsp1109I GCAGC 3 cut(s) 569, 688, 691
LweI GCATC 6 cut(s) 197, 211, 249, 412, 478, 886
MaeI CTAG 3 cut(s) 528, 771, 888
MaeIII GTNAC 2 cut(s) 169, 574
MalI GATC 2 cut(s) 669, 792
MboI GATC 2 cut(s) 667, 790
MboII GAAGA 2 cut(s) 517, 776
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 4 cut(s) 144, 286, 805, 814
MnlI CCTC 8 cut(s) 24, 279, 343, 347, 397, 400, 460, 769
MroI TCCGGA 1 cut(s) 868
MseI TTAA 5 cut(s) 21, 75, 290, 339, 919
MslI CAYNNNNRTG 4 cut(s) 111, 207, 560, 663
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 4 cut(s) 59, 725, 843, 869
MspR9I CCNGG 1 cut(s) 843
Mva1269I GAATGC 1 cut(s) 863
MwoI GCNNNNNNNGC 3 cut(s) 55, 118, 698
NciI CCSGG 1 cut(s) 843
NdeII GATC 2 cut(s) 667, 790
NlaIII CATG 7 cut(s) 116, 210, 260, 617, 643, 662, 764
NlaIV GGNNCC 1 cut(s) 267
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 2 cut(s) 169, 574
NspI RCATGY 3 cut(s) 617, 643, 662
PaeR7I CTCGAG 1 cut(s) 444
PciI ACATGT 1 cut(s) 658
PctI GAATGC 1 cut(s) 863
PfeI GAWTC 4 cut(s) 476, 531, 569, 767
PkrI GCNGC 3 cut(s) 584, 703, 706
PscI ACATGT 1 cut(s) 658
Psp124BI GAGCTC 1 cut(s) 461
PspEI GGTNACC 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 267
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 4 cut(s) 374, 517, 538, 840
RsaNI GTAC 4 cut(s) 373, 516, 537, 839
RseI CAYNNNNRTG 4 cut(s) 111, 207, 560, 663
SacI GAGCTC 1 cut(s) 461
SaqAI TTAA 5 cut(s) 21, 75, 290, 339, 919
SatI GCNGC 3 cut(s) 583, 702, 705
Sau3AI GATC 2 cut(s) 667, 790
ScrFI CCNGG 1 cut(s) 843
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 6 cut(s) 197, 211, 249, 412, 478, 886
SfcI CTRYAG 1 cut(s) 245
Sfr274I CTCGAG 1 cut(s) 444
SlaI CTCGAG 1 cut(s) 444
SmiMI CAYNNNNRTG 4 cut(s) 111, 207, 560, 663
SmlI CTYRAG 1 cut(s) 444
SmoI CTYRAG 1 cut(s) 444
Sse9I AATT 4 cut(s) 144, 286, 805, 814
SsiI CCGC 2 cut(s) 17, 119
SspMI CTAG 3 cut(s) 528, 771, 888
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 1 cut(s) 841
TaaI ACNGT 3 cut(s) 81, 232, 372
TaqI TCGA 1 cut(s) 445
TaqII GACCGA 1 cut(s) 562
TasI AATT 4 cut(s) 144, 286, 805, 814
TatI WGTACW 2 cut(s) 372, 515
TfiI GAWTC 4 cut(s) 476, 531, 569, 767
Tru1I TTAA 5 cut(s) 21, 75, 290, 339, 919
Tru9I TTAA 5 cut(s) 21, 75, 290, 339, 919
TscAI CASTG 3 cut(s) 204, 235, 583
TseFI GTSAC 2 cut(s) 169, 574
TseI GCWGC 3 cut(s) 582, 701, 704
Tsp45I GTSAC 2 cut(s) 169, 574
TspDTI ATGAA 5 cut(s) 58, 129, 353, 777, 870
TspRI CASTG 3 cut(s) 204, 235, 583
XapI RAATTY 3 cut(s) 144, 286, 814
XceI RCATGY 3 cut(s) 617, 643, 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XhoI CTCGAG 1 cut(s) 444
XspI CTAG 3 cut(s) 528, 771, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.