MD14G1151500.v1.1

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009164
Physical Location & Seq
Reverse (-)
24486788 .. 24490270
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1151500.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGAAGAGGACGAGGGAAGACAAGGCCATGGAGATCGTGTGGCAGAGTCCGGCGATTCGGCCGCAACCGCAAGATTACATCTTTCGCGACGGGCGGCGCCATGTCAGACCCTACTACTTCGAGTTCATCTGCCATGTGAAGAATCGGTGGGCGGGGAAGACCATCGTTGATTTGTTCACTCATGAGTTCAAAGGCCGGCCTTATGATTACTATGTTAGTGCAGTGAAATCTGGAAGGATACAGGTTGATGGAGAGATGGTGCCGGTTTCCCACATTGTTAAAACGTCGCAAAAGATAAGCCACTTCGTCCACAGGCATGAACCGCCAGTCATGACTTGGGATATATCGATTCTTCAAGAGAAACCGGATGTGGTTGTTGTTTGTAAACCTGCATCTGTTCCGGTGCATCCGTGTGGTCAATATCGTAAGAACACCGTTGTTGGCATCCTTGAGGCGGAGCATGGCTTGGCACCTCTATTTCCAATTCATCGACTAGATCGCCTTGTATCAGGACTCCTTATCTTGGCCAGAAGTGCTTCAAAAGCCGACTATTTTAGGCAACAGATTGAAGGAGGGATGGTCCAGAAACAGTACATTGCAAGAGTTGCTGGGGAATTTCCTGAGCAAGAGCAAGTTGTAGATGCTAATGTTAATCATGATGCTCGAGAATGTAGGAGCACAACAGAGATTAGTCAGTCAGGTGATGATACTCCCATCAAGGGGAAGACTGCCTGTACAAAGTTTACTAGACTCAGTACCAATGGAATCCATAGTCTTGTCTTGTGCGAGCCAGTCACTGGCCGAACTCATCAAATACGTGTCCATTTGCAACATTTAGGCTATCCGATTGCCAATGACGTGCTTTACCTGTCCAAACATGTTAATGGTCGTTCAAGTGAAGGAATGGGTGCTGATAGAGCTGCTGCAAATTCCAGCCCCTCTCCGCCATCTGGCTTTCGCGAAAATTTATCCACTGAACCTGTAGATTCCAACGAAGATTTCAGCATTGATCCCATGTGTACAAATTGTCCGAATTTGGTTCCAAAAGGGTACGATGTTTATGAAGAGGCTTTGTGGCTACACTGTGTTCGGTATTCCGCACCGGGATGGACTTATGAATGCCCATATCCTGATTGGGCATCACTTACCTAA

Protein Analysis

384

Amino Acids

43.33

Weight (kDa)

7.26

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 123 - 278 5e-30 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 3 cut(s) 96, 259, 469
AccB7I CCANNNNNTGG 1 cut(s) 797
AccII CGCG 2 cut(s) 87, 960
AciI CCGC 8 cut(s) 62, 68, 94, 152, 323, 455, 944, 1098
AclWI GGATC 1 cut(s) 1004
AcoI YGGCCR 3 cut(s) 59, 525, 799
AcsI RAATTY 4 cut(s) 614, 928, 964, 1033
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 5 cut(s) 593, 736, 757, 1021, 1052
AfiI CCNNNNNNNGG 6 cut(s) 454, 523, 719, 720, 797, 950
AflIII ACRYGT 2 cut(s) 817, 877
AgsI TTSAA 5 cut(s) 190, 356, 540, 569, 894
AjiI CACGTC 1 cut(s) 859
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
AluBI AGCT 1 cut(s) 920
AluI AGCT 1 cut(s) 920
Alw21I GWGCWC 1 cut(s) 680
AlwI GGATC 1 cut(s) 1004
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 663
AoxI GGCC 6 cut(s) 24, 59, 193, 197, 525, 799
ApeKI GCWGC 2 cut(s) 920, 923
ApoI RAATTY 4 cut(s) 614, 928, 964, 1033
Asp700I GAANNNNTTC 1 cut(s) 535
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 580
AsuC2I CCSGG 1 cut(s) 1104
AsuHPI GGTGA 1 cut(s) 713
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 580
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 3 cut(s) 96, 259, 469
BbsI GAAGAC 3 cut(s) 24, 164, 731
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 2 cut(s) 907, 910
BccI CCATC 7 cut(s) 170, 242, 250, 571, 722, 955, 1101
BciVI GTATCC 1 cut(s) 231
BcnI CCSGG 1 cut(s) 1104
BfaI CTAG 2 cut(s) 494, 747
BfmI CTRYAG 1 cut(s) 981
BfoI RGCGCY 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 397
BfuI GTATCC 1 cut(s) 231
BisI GCNGC 4 cut(s) 62, 95, 921, 924
BlsI GCNGC 4 cut(s) 63, 96, 922, 925
Bme1390I CCNGG 1 cut(s) 1104
Bme18I GGWCC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 663
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 4 cut(s) 98, 261, 471, 1041
BmrFI CCNGG 1 cut(s) 1104
BmsI GCATC 6 cut(s) 401, 415, 453, 631, 649, 1148
BpiI GAAGAC 3 cut(s) 24, 164, 731
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 1104
Bsa29I ATCGAT 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 818
BsaHI GRCGYC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 2 cut(s) 364, 400
BsaXI ACNNNNNCTCC 6 cut(s) 23, 53, 243, 273, 564, 594
Bsc4I CCNNNNNNNGG 6 cut(s) 454, 523, 719, 720, 797, 950
Bse118I RCCGGY 2 cut(s) 195, 262
Bse1I ACTGG 3 cut(s) 326, 791, 802
Bse3DI GCAATG 1 cut(s) 594
BseCI ATCGAT 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 5 cut(s) 373, 406, 444, 582, 1112
BseLI CCNNNNNNNGG 6 cut(s) 454, 523, 719, 720, 797, 950
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 2 cut(s) 612, 766
BseNI ACTGG 3 cut(s) 326, 791, 802
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 907, 910
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 2 cut(s) 87, 960
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 6 cut(s) 26, 61, 195, 199, 527, 801
BshNI GGYRCC 3 cut(s) 96, 259, 469
BshVI ATCGAT 1 cut(s) 347
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 6 cut(s) 50, 196, 263, 365, 401, 1103
BslI CCNNNNNNNGG 6 cut(s) 454, 523, 719, 720, 797, 950
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 6 cut(s) 26, 61, 195, 199, 527, 801
BsoBI CYCGRG 1 cut(s) 663
Bsp1286I GDGCHC 1 cut(s) 680
Bsp1407I TGTACA 2 cut(s) 734, 1019
Bsp143I GATC 3 cut(s) 33, 496, 1009
Bsp19I CCATGG 1 cut(s) 27
Bsp68I TCGCGA 2 cut(s) 87, 960
BspACI CCGC 8 cut(s) 62, 68, 94, 152, 323, 455, 944, 1098
BspANI GGCC 6 cut(s) 26, 61, 195, 199, 527, 801
BspCNI CTCAG 2 cut(s) 613, 765
BspDI ATCGAT 1 cut(s) 347
BspFNI CGCG 2 cut(s) 87, 960
BspHI TCATGA 3 cut(s) 181, 330, 655
BspLI GGNNCC 4 cut(s) 98, 261, 471, 1041
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 1004
BspT107I GGYRCC 3 cut(s) 96, 259, 469
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 2 cut(s) 195, 262
BsrGI TGTACA 2 cut(s) 734, 1019
BsrI ACTGG 3 cut(s) 326, 791, 802
BssAI RCCGGY 2 cut(s) 195, 262
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 33, 496, 1009
BssNI GRCGYC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 436, 591, 1085
Bst6I CTCTTC 1 cut(s) 1059
BstACI GRCGYC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 605
BstAUI TGTACA 2 cut(s) 734, 1019
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 197, 788
BstDEI CTNAG 2 cut(s) 621, 752
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 5 cut(s) 373, 406, 444, 582, 1112
BstFNI CGCG 2 cut(s) 87, 960
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 3 cut(s) 36, 499, 1012
BstMBI GATC 3 cut(s) 33, 496, 1009
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 6 cut(s) 67, 322, 533, 542, 605, 917
BstNSI RCATGY 1 cut(s) 881
BstSCI CCNGG 1 cut(s) 1102
BstSFI CTRYAG 1 cut(s) 981
BstUI CGCG 2 cut(s) 87, 960
BstV1I GCAGC 2 cut(s) 907, 910
BstV2I GAAGAC 3 cut(s) 24, 164, 731
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 347
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 6 cut(s) 26, 61, 195, 199, 527, 801
BsuTUI ATCGAT 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 5 cut(s) 373, 406, 444, 582, 1112
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 4 cut(s) 228, 795, 972, 1081
BtuMI TCGCGA 2 cut(s) 87, 960
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 197, 788
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 3 cut(s) 181, 330, 655
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 2 cut(s) 195, 262
Cfr13I GGNCC 1 cut(s) 580
ClaI ATCGAT 1 cut(s) 347
Csp6I GTAC 5 cut(s) 592, 735, 756, 1020, 1051
CviQI GTAC 5 cut(s) 592, 735, 756, 1020, 1051
DdeI CTNAG 2 cut(s) 621, 752
DinI GGCGCC 1 cut(s) 98
DpnI GATC 3 cut(s) 35, 498, 1011
DpnII GATC 3 cut(s) 33, 496, 1009
EaeI YGGCCR 3 cut(s) 59, 525, 799
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 1059
EarI CTCTTC 1 cut(s) 1059
EciI GGCGGA 2 cut(s) 470, 933
EclXI CGGCCG 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 580
Eco52I CGGCCG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 27
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 27
FauI CCCGC 1 cut(s) 145
Fnu4HI GCNGC 4 cut(s) 62, 95, 921, 924
FokI GGATG 5 cut(s) 380, 393, 431, 589, 1119
FseI GGCCGGCC 1 cut(s) 199
Fsp4HI GCNGC 4 cut(s) 62, 95, 921, 924
FspBI CTAG 2 cut(s) 494, 747
GlaI GCGC 1 cut(s) 98
GluI GCNGC 4 cut(s) 62, 95, 921, 924
GsaI CCCAGC 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 6 cut(s) 26, 61, 195, 199, 527, 801
HapII CCGG 6 cut(s) 50, 196, 263, 365, 401, 1103
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 97
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 8 cut(s) 46, 55, 142, 349, 513, 750, 765, 986
HpaII CCGG 6 cut(s) 50, 196, 263, 365, 401, 1103
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 5 cut(s) 177, 310, 386, 744, 1020
Hpy188I TCNGA 3 cut(s) 107, 846, 1032
Hpy8I GTNNAC 5 cut(s) 177, 310, 386, 744, 1020
Hpy99I CGWCG 2 cut(s) 92, 289
HpyAV CCTTC 3 cut(s) 228, 563, 893
HpyCH4III ACNGT 3 cut(s) 436, 591, 1085
HpyCH4IV ACGT 3 cut(s) 284, 817, 858
HpyCH4V TGCA 6 cut(s) 221, 392, 406, 599, 829, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 322, 533, 542, 605, 917
HpyF3I CTNAG 2 cut(s) 621, 752
HpySE526I ACGT 3 cut(s) 284, 817, 858
Hsp92I GRCGYC 1 cut(s) 97
HspAI GCGC 1 cut(s) 97
KasI GGCGCC 1 cut(s) 96
KroI GCCGGC 1 cut(s) 195
KroNI GCCGGC 1 cut(s) 197
Kzo9I GATC 3 cut(s) 33, 496, 1009
LmnI GCTCC 2 cut(s) 457, 675
Lsp1109I GCAGC 2 cut(s) 907, 910
LweI GCATC 6 cut(s) 401, 415, 453, 631, 649, 1148
MaeI CTAG 2 cut(s) 494, 747
MaeII ACGT 3 cut(s) 284, 817, 858
MaeIII GTNAC 1 cut(s) 793
MalI GATC 3 cut(s) 35, 498, 1011
MboI GATC 3 cut(s) 33, 496, 1009
MboII GAAGA 8 cut(s) 16, 29, 151, 169, 344, 736, 1007, 1076
MhlI GDGCHC 1 cut(s) 680
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 6 cut(s) 483, 614, 928, 964, 1024, 1033
MluNI TGGCCA 1 cut(s) 527
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 3 cut(s) 55, 507, 744
MmeI TCCRAC 1 cut(s) 1014
MnlI CCTC 6 cut(s) 6, 445, 483, 566, 949, 1060
Mox20I TGGCCA 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 535
MscI TGGCCA 1 cut(s) 527
MseI TTAA 3 cut(s) 279, 651, 882
MslI CAYNNNNRTG 4 cut(s) 315, 411, 882, 1105
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 6 cut(s) 50, 196, 263, 365, 401, 1103
MspR9I CCNGG 1 cut(s) 1104
Mva1269I GAATGC 1 cut(s) 1124
MvnI CGCG 2 cut(s) 87, 960
MwoI GCNNNNNNNGC 6 cut(s) 67, 322, 533, 542, 605, 917
NaeI GCCGGC 1 cut(s) 197
NarI GGCGCC 1 cut(s) 97
NciI CCSGG 1 cut(s) 1104
NcoI CCATGG 1 cut(s) 27
NdeII GATC 3 cut(s) 33, 496, 1009
NgoMIV GCCGGC 1 cut(s) 195
NlaIV GGNNCC 4 cut(s) 98, 261, 471, 1041
NmuCI GTSAC 1 cut(s) 793
NruI TCGCGA 2 cut(s) 87, 960
NspI RCATGY 1 cut(s) 881
PaeR7I CTCGAG 1 cut(s) 663
PagI TCATGA 3 cut(s) 181, 330, 655
PciI ACATGT 1 cut(s) 877
PctI GAATGC 1 cut(s) 1124
PdiI GCCGGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 5 cut(s) 55, 142, 349, 765, 986
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 4 cut(s) 63, 96, 922, 925
PleI GAGTC 3 cut(s) 54, 507, 744
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 3 cut(s) 54, 507, 744
Ppu21I YACGTR 1 cut(s) 818
PscI ACATGT 1 cut(s) 877
PspFI CCCAGC 1 cut(s) 608
PspN4I GGNNCC 4 cut(s) 98, 261, 471, 1041
PspPI GGNCC 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 797
RigI GGCCGGCC 1 cut(s) 199
RruI TCGCGA 2 cut(s) 87, 960
RsaI GTAC 5 cut(s) 593, 736, 757, 1021, 1052
RsaNI GTAC 5 cut(s) 592, 735, 756, 1020, 1051
RseI CAYNNNNRTG 4 cut(s) 315, 411, 882, 1105
SaqAI TTAA 3 cut(s) 279, 651, 882
SatI GCNGC 4 cut(s) 62, 95, 921, 924
Sau3AI GATC 3 cut(s) 33, 496, 1009
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 3 cut(s) 55, 507, 744
ScrFI CCNGG 1 cut(s) 1104
SduI GDGCHC 1 cut(s) 680
SfaNI GCATC 6 cut(s) 401, 415, 453, 631, 649, 1148
SfcI CTRYAG 1 cut(s) 981
SfoI GGCGCC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 580
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 4 cut(s) 315, 411, 882, 1105
SmlI CTYRAG 2 cut(s) 449, 663
SmoI CTYRAG 2 cut(s) 449, 663
Sse9I AATT 6 cut(s) 483, 614, 928, 964, 1024, 1033
SsiI CCGC 8 cut(s) 62, 68, 94, 152, 323, 455, 944, 1098
SspDI GGCGCC 1 cut(s) 96
SspMI CTAG 2 cut(s) 494, 747
StyD4I CCNGG 1 cut(s) 1102
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 436, 591, 1085
TaiI ACGT 3 cut(s) 287, 820, 861
TaqI TCGA 4 cut(s) 120, 347, 490, 664
TasI AATT 6 cut(s) 483, 614, 928, 964, 1024, 1033
TatI WGTACW 3 cut(s) 591, 734, 1019
TauI GCSGC 2 cut(s) 64, 97
TfiI GAWTC 5 cut(s) 55, 142, 349, 765, 986
Tru1I TTAA 3 cut(s) 279, 651, 882
Tru9I TTAA 3 cut(s) 279, 651, 882
TscAI CASTG 4 cut(s) 228, 802, 979, 1088
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 2 cut(s) 920, 923
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 6 cut(s) 17, 115, 333, 476, 1077, 1131
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 4 cut(s) 228, 802, 979, 1088
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 4 cut(s) 614, 928, 964, 1033
XceI RCATGY 1 cut(s) 881
XcmI CCANNNNNNNNNTGG 2 cut(s) 333, 1131
XhoI CTCGAG 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 2 cut(s) 494, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.