AT5G52100

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012204
Physical Location & Seq
Forward (+)
21170048 .. 21172346
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52100.1

Sequence Viewer

Length: 897 bp
ATGGCAGCTGTGAACTGCCATTTCTTCCAGCTATCACGACATCTCAAACCTTCTAGGCCTTCTTTTTCCTGCTCTGCTTCTCAACCTTCACAAAACAACATTAAGGTAATCATAAATGGAGCTGCTAAGGAAATAGGTAGAGCGGCTGTGGTGGCTGTGACTAAGGCTAGGGGAATGGAGTTAGCTGGTGCTGTCGATAATCATTTTGTTGGCGAAGATATTGGCTTGCTGTGTGACATGGAGGAGCCTTTAGAGATACCTGTAGTGAGTGATCTAACAATGGTGTTAGGCTCAATATCTCAGGGAAAAGAAGTCGGAGTTGTGATCGACTTTACAGACCCATCAACAGTGTATGAGAATGTAAAACAGGCAACGGCATTTGGGATGAAGAGTGTGGTGTATGTACCTCGGATTAAGCCAGAGACAGTATCTGCGTTGTCTGCACTATGTGACAAGGCCACCATGGGATGTCTTGTAGCACCAACTCTATCCATAGGATCTATACTTCTCCAACAGGCTGTGATCATGGCCTCCTTCCACTACAACAATGTAGAGCTAGTGGAGTCAAGGCCTAATGCAGCGGATCTTCCTTCTCCTGAAGCTATCCAAATTGCAAACAACATATCAAATCTTGGTCAGATCTACAACAGAGAGGATAGTTCAACTGATGTTCAGGCAAGAGGTCAAGTAATCGGAGAAGACGGGGTTCGTGTTCACAGTATGGTTCTACCAGGACTACCATCTAGTACACAAGTCTACTTCTCAAGTCCAGGAGACGTTTACACTGTTAAACATGACATAATTGACGTAAGGTCCCTAATGCCCGGCCTACTCCTAGCAATCAGAAAGGTGGTACGCCTCAAGAATCTGGTTTATGGCCTCGAGAAATTTCTGTAG

Protein Analysis

298

Amino Acids

32.04

Weight (kDa)

5.88

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DapB_N PF01113 34 - 161 1.4e-15 Dihydrodipicolinate reductase, N-terminus
DapB_C PF05173 168 - 298 4.1e-18 Dihydrodipicolinate reductase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52100
fragaria_vesca FvH4_7g29020 FvH4_7g29020
malus_domestica MD01G1197000.v1.1 MD01G1197100.v1.1 MD07G1264500.v1.1
prunus_persica Prupe.2G290400_v2.0.a1
pyrus_communis pycom01g20750 pycom07g24300
rosa_chinensis RchiOBHm_Chr1g0376731
rosa_laevigata RLG00000026575
rosa_multiflora Rmu_sc0003609.1_g000003
rosa_roxburghii Rroxscaffold_4G00281840
rosa_rugosa Rorug01G0397900
rosa_samantha Rh1AG415200 Rh1BG374800 Rh1CG388600
rosa_wichuraiana Rw0G004560 Rw1G036400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 756
AciI CCGC 2 cut(s) 143, 581
AclWI GGATC 2 cut(s) 505, 591
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 1 cut(s) 618
AdeI CACNNNGTG 1 cut(s) 449
AfaI GTAC 3 cut(s) 405, 748, 855
AgsI TTSAA 1 cut(s) 663
AhdI GACNNNNNGTC 1 cut(s) 811
AjnI CCWGG 2 cut(s) 730, 769
AluBI AGCT 6 cut(s) 8, 31, 122, 185, 556, 602
AluI AGCT 6 cut(s) 8, 31, 122, 185, 556, 602
Alw26I GTCTC 2 cut(s) 416, 768
AlwI GGATC 2 cut(s) 505, 591
AlwNI CAGNNNCTG 1 cut(s) 431
Ama87I CYCGRG 1 cut(s) 881
AoxI GGCC 6 cut(s) 56, 456, 528, 569, 826, 877
ApeKI GCWGC 3 cut(s) 5, 122, 578
ApoI RAATTY 1 cut(s) 887
AspS9I GGNCC 1 cut(s) 813
AsuC2I CCSGG 1 cut(s) 825
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 1 cut(s) 813
BbsI GAAGAC 1 cut(s) 705
BbvI GCAGC 3 cut(s) 17, 109, 590
BccI CCATC 2 cut(s) 349, 748
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 2 cut(s) 732, 771
BclI TGATCA 1 cut(s) 522
BcnI CCSGG 1 cut(s) 825
BcoDI GTCTC 2 cut(s) 416, 768
BfaI CTAG 5 cut(s) 54, 168, 557, 744, 836
BfmI CTRYAG 2 cut(s) 261, 893
BglII AGATCT 1 cut(s) 639
BisI GCNGC 4 cut(s) 6, 123, 144, 579
BlsI GCNGC 4 cut(s) 7, 124, 145, 580
Bme1390I CCNGG 3 cut(s) 732, 771, 825
Bme18I GGWCC 1 cut(s) 813
BmeRI GACNNNNNGTC 1 cut(s) 811
BmeT110I CYCGRG 1 cut(s) 881
BmgT120I GGNCC 1 cut(s) 813
BmiI GGNNCC 2 cut(s) 246, 815
BmrFI CCNGG 3 cut(s) 732, 771, 825
BpiI GAAGAC 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 748, 845
BpuMI CCSGG 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 407, 462
BseBI CCWGG 2 cut(s) 732, 771
BseDI CCNNGG 2 cut(s) 407, 462
BseGI GGATG 2 cut(s) 390, 473
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 3 cut(s) 17, 109, 590
BsgI GTGCAG 1 cut(s) 426
BshFI GGCC 6 cut(s) 58, 458, 530, 571, 828, 879
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 1 cut(s) 825
BslFI GGGAC 1 cut(s) 799
BsmAI GTCTC 2 cut(s) 416, 768
BsmBI CGTCTC 1 cut(s) 768
BsmFI GGGAC 1 cut(s) 799
BsnI GGCC 6 cut(s) 58, 458, 530, 571, 828, 879
BsoBI CYCGRG 1 cut(s) 881
Bsp143I GATC 6 cut(s) 271, 324, 497, 522, 583, 639
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 2 cut(s) 143, 581
BspANI GGCC 6 cut(s) 58, 458, 530, 571, 828, 879
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 246, 815
BspPI GGATC 2 cut(s) 505, 591
BsrBI CCGCTC 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 407, 462
BssMI GATC 6 cut(s) 271, 324, 497, 522, 583, 639
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 2 cut(s) 732, 771
Bst4CI ACNGT 4 cut(s) 349, 427, 719, 787
Bst6I CTCTTC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 126, 162, 300
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 2 cut(s) 390, 473
BstKTI GATC 6 cut(s) 274, 327, 500, 525, 586, 642
BstMAI GTCTC 2 cut(s) 416, 768
BstMBI GATC 6 cut(s) 271, 324, 497, 522, 583, 639
BstMWI GCNNNNNNNGC 2 cut(s) 152, 440
BstNI CCWGG 2 cut(s) 732, 771
BstSCI CCNGG 3 cut(s) 730, 769, 823
BstSFI CTRYAG 2 cut(s) 261, 893
BstV1I GCAGC 3 cut(s) 17, 109, 590
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 3 cut(s) 497, 583, 639
BstYI RGATCY 3 cut(s) 497, 583, 639
BsuRI GGCC 6 cut(s) 58, 458, 530, 571, 828, 879
BtgI CCRYGG 1 cut(s) 462
BtsCI GGATG 2 cut(s) 390, 473
BtsIMutI CAGTG 2 cut(s) 354, 783
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 813
Csp6I GTAC 3 cut(s) 404, 747, 854
CviAII CATG 4 cut(s) 238, 463, 526, 794
CviQI GTAC 3 cut(s) 404, 747, 854
DdeI CTNAG 3 cut(s) 126, 162, 300
DpnI GATC 6 cut(s) 273, 326, 499, 524, 585, 641
DpnII GATC 6 cut(s) 271, 324, 497, 522, 583, 639
DraIII CACNNNGTG 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 383
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 462
Eco147I AGGCCT 2 cut(s) 58, 571
Eco47I GGWCC 1 cut(s) 813
Eco57I CTGAAG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 881
EcoO109I RGGNCCY 1 cut(s) 813
EcoRII CCWGG 2 cut(s) 730, 769
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 768
FaeI CATG 4 cut(s) 241, 466, 529, 797
FaqI GGGAC 1 cut(s) 799
FatI CATG 4 cut(s) 237, 462, 525, 793
FbaI TGATCA 1 cut(s) 522
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 4 cut(s) 6, 123, 144, 579
FokI GGATG 2 cut(s) 397, 480
Fsp4HI GCNGC 4 cut(s) 6, 123, 144, 579
FspBI CTAG 5 cut(s) 54, 168, 557, 744, 836
GluI GCNGC 4 cut(s) 6, 123, 144, 579
HaeIII GGCC 6 cut(s) 58, 458, 530, 571, 828, 879
HapII CCGG 1 cut(s) 825
Hin1II CATG 4 cut(s) 241, 466, 529, 797
HinfI GANTC 2 cut(s) 563, 865
HpaII CCGG 1 cut(s) 825
Hpy166II GTNNAC 5 cut(s) 13, 715, 749, 757, 781
Hpy188I TCNGA 5 cut(s) 317, 411, 639, 695, 845
Hpy188III TCNNGA 4 cut(s) 36, 596, 862, 883
Hpy8I GTNNAC 5 cut(s) 13, 715, 749, 757, 781
HpyAV CCTTC 5 cut(s) 60, 69, 96, 544, 600
HpyCH4III ACNGT 4 cut(s) 349, 427, 719, 787
HpyCH4IV ACGT 2 cut(s) 777, 807
HpyCH4V TGCA 3 cut(s) 443, 578, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 440
HpyF3I CTNAG 3 cut(s) 126, 162, 300
HpySE526I ACGT 2 cut(s) 777, 807
Hsp92II CATG 4 cut(s) 241, 466, 529, 797
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 6 cut(s) 271, 324, 497, 522, 583, 639
LmnI GCTCC 2 cut(s) 119, 244
Lsp1109I GCAGC 3 cut(s) 17, 109, 590
MaeI CTAG 5 cut(s) 54, 168, 557, 744, 836
MaeII ACGT 2 cut(s) 777, 807
MaeIII GTNAC 3 cut(s) 157, 233, 449
MalI GATC 6 cut(s) 273, 326, 499, 524, 585, 641
MbiI CCGCTC 1 cut(s) 143
MboI GATC 6 cut(s) 271, 324, 497, 522, 583, 639
MboII GAAGA 5 cut(s) 16, 227, 400, 578, 710
MflI RGATCY 3 cut(s) 497, 583, 639
MluCI AATT 3 cut(s) 609, 801, 887
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 2 cut(s) 295, 535
MnlI CCTC 7 cut(s) 235, 417, 541, 646, 674, 869, 890
MseI TTAA 3 cut(s) 102, 414, 789
MspA1I CMGCKG 2 cut(s) 8, 581
MspI CCGG 1 cut(s) 825
MspR9I CCNGG 3 cut(s) 732, 771, 825
MvaI CCWGG 2 cut(s) 732, 771
MwoI GCNNNNNNNGC 2 cut(s) 152, 440
NciI CCSGG 1 cut(s) 825
NcoI CCATGG 1 cut(s) 462
NdeII GATC 6 cut(s) 271, 324, 497, 522, 583, 639
NlaIII CATG 4 cut(s) 241, 466, 529, 797
NlaIV GGNNCC 2 cut(s) 246, 815
NmuCI GTSAC 3 cut(s) 157, 233, 449
PaeR7I CTCGAG 1 cut(s) 881
PceI AGGCCT 2 cut(s) 58, 571
PfeI GAWTC 1 cut(s) 865
PfoI TCCNGGA 1 cut(s) 769
PkrI GCNGC 4 cut(s) 7, 124, 145, 580
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
PpuMI RGGWCCY 1 cut(s) 813
Psp5II RGGWCCY 1 cut(s) 813
Psp6I CCWGG 2 cut(s) 730, 769
PspGI CCWGG 2 cut(s) 730, 769
PspN4I GGNNCC 2 cut(s) 246, 815
PspPI GGNCC 1 cut(s) 813
PspPPI RGGWCCY 1 cut(s) 813
PstNI CAGNNNCTG 1 cut(s) 431
PsuI RGATCY 3 cut(s) 497, 583, 639
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 3 cut(s) 405, 748, 855
RsaNI GTAC 3 cut(s) 404, 747, 854
SaqAI TTAA 3 cut(s) 102, 414, 789
SatI GCNGC 4 cut(s) 6, 123, 144, 579
Sau3AI GATC 6 cut(s) 271, 324, 497, 522, 583, 639
Sau96I GGNCC 1 cut(s) 813
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 3 cut(s) 732, 771, 825
SfcI CTRYAG 2 cut(s) 261, 893
Sfr274I CTCGAG 1 cut(s) 881
SinI GGWCC 1 cut(s) 813
SlaI CTCGAG 1 cut(s) 881
SmlI CTYRAG 3 cut(s) 763, 860, 881
SmoI CTYRAG 3 cut(s) 763, 860, 881
Sse9I AATT 3 cut(s) 609, 801, 887
SseBI AGGCCT 2 cut(s) 58, 571
SsiI CCGC 2 cut(s) 143, 581
SspMI CTAG 5 cut(s) 54, 168, 557, 744, 836
StuI AGGCCT 2 cut(s) 58, 571
StyD4I CCNGG 3 cut(s) 730, 769, 823
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 4 cut(s) 349, 427, 719, 787
TaiI ACGT 2 cut(s) 780, 810
TaqI TCGA 3 cut(s) 195, 327, 882
TasI AATT 3 cut(s) 609, 801, 887
TatI WGTACW 1 cut(s) 746
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 1 cut(s) 865
Tru1I TTAA 3 cut(s) 102, 414, 789
Tru9I TTAA 3 cut(s) 102, 414, 789
TscAI CASTG 2 cut(s) 354, 790
TseFI GTSAC 3 cut(s) 157, 233, 449
TseI GCWGC 3 cut(s) 5, 122, 578
Tsp45I GTSAC 3 cut(s) 157, 233, 449
TspDTI ATGAA 1 cut(s) 401
TspRI CASTG 2 cut(s) 354, 790
VpaK11BI GGWCC 1 cut(s) 813
XapI RAATTY 1 cut(s) 887
XhoI CTCGAG 1 cut(s) 881
XmiI GTMKAC 1 cut(s) 756
XspI CTAG 5 cut(s) 54, 168, 557, 744, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.