Rw0G004560

Dihydrodipicolinate reductase, C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00171
OG0012204
Physical Location & Seq
Forward (+)
68 .. 2060
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004560.1

Sequence Viewer

Length: 864 bp
ATGGTAGCTTTGAGCTGCCAGTTTCAACCTGTGGTCTGCAAGAGTTATCGGAATGGTAGAGCTAGACCTTCTGCTCTTTGCTCAATGCAACCGTCTCAAAACAATATCAAGGTAATAATAAATGGAGCTGCAAAGGAAATAGGGAGGGCAGCAGTGATGGCCGTGACCAGAGCTCGAGGGATGGAGGTGGCCGGTGCAGTGGATTCGTATCTTCCATTGGAGATACCGTTAACGAATGATCTCACCATGGTCTTAGGTTCCATATCGCAGTCTAAAGCAACAGGAGTAGTTGTGGATTTCACTGACCCTTCCAAAGTTTATGACAATGTGAAGCAGGCAACAGCATTTGGAATGAGGAGTGTGGTTTATGTGCCAAAAATTAAGTTAGATACAGTATCAGCATTATCTGCACTCTGTGAGAAAGCCAGCATGGGTTGTCTTGTCGCACCGACTCTTTCTATTGGATCTATACTCCTCCAACAGGCTGCAATTTCAGCTTCCTTTCACTATAACAATGTAGAAATTGTAGAATCAAGAGCTAATGCAACGGATCTTCCATCATCAGATGCAACCCAAATTGCCAACAACCTCTCTAACCTTGGCCAGATCTATAACAGAGAAGACATTTCAACAGACGTTAAGGCAAGGGGTCAAGTTTTGGGTGAAGATGGAGTTCGTGTGCATAGCTTAGTCCTTCCAGGGCTTCCCTCCAGTACAACAGTCTACTTCTCTCGCCCAGGAGAGGTTTACACTCTGAAACATGATATCACAGATGTGCAAAGTCTCATGCCAGGCCTAATCTTAGCTATCAGAAAGATTGTTCGCATCAAGAGCCTGGTGTATGGCCTGGAGAAACTTTTATGA

Protein Analysis

287

Amino Acids

30.74

Weight (kDa)

8.92

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DapB_N PF01113 36 - 150 5.7e-09 Dihydrodipicolinate reductase, N-terminus
DapB_C PF05173 157 - 287 3.7e-19 Dihydrodipicolinate reductase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52100
fragaria_vesca FvH4_7g29020 FvH4_7g29020
malus_domestica MD01G1197000.v1.1 MD01G1197100.v1.1 MD07G1264500.v1.1
prunus_persica Prupe.2G290400_v2.0.a1
pyrus_communis pycom01g20750 pycom07g24300
rosa_chinensis RchiOBHm_Chr1g0376731
rosa_laevigata RLG00000026575
rosa_multiflora Rmu_sc0003609.1_g000003
rosa_roxburghii Rroxscaffold_4G00281840
rosa_rugosa Rorug01G0397900
rosa_samantha Rh1AG415200 Rh1BG374800 Rh1CG388600
rosa_wichuraiana Rw0G004560 Rw1G036400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 723
AclWI GGATC 2 cut(s) 472, 558
AcoI YGGCCR 3 cut(s) 159, 189, 601
AdeI CACNNNGTG 1 cut(s) 416
AfaI GTAC 1 cut(s) 715
AfiI CCNNNNNNNGG 2 cut(s) 481, 742
AgsI TTSAA 2 cut(s) 26, 630
AjnI CCWGG 5 cut(s) 697, 736, 790, 834, 846
AleI CACNNNNGTG 1 cut(s) 773
AluBI AGCT 9 cut(s) 8, 15, 62, 128, 173, 497, 539, 687, 806
AluI AGCT 9 cut(s) 8, 15, 62, 128, 173, 497, 539, 687, 806
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 2 cut(s) 99, 788
AlwI GGATC 2 cut(s) 472, 558
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 5 cut(s) 159, 189, 601, 793, 844
ApeKI GCWGC 4 cut(s) 15, 128, 149, 485
AsuHPI GGTGA 2 cut(s) 235, 674
AvaI CYCGRG 1 cut(s) 174
BalI TGGCCA 1 cut(s) 603
BanII GRGCYC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 4 cut(s) 2, 115, 161, 472
BccI CCATC 4 cut(s) 151, 175, 565, 662
BceAI ACGGC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 186, 220
BciT130I CCWGG 5 cut(s) 699, 738, 792, 836, 848
BcoDI GTCTC 2 cut(s) 99, 788
BfaI CTAG 1 cut(s) 63
BglII AGATCT 1 cut(s) 606
BisI GCNGC 4 cut(s) 16, 129, 150, 486
BlsI GCNGC 4 cut(s) 17, 130, 151, 487
Bme1390I CCNGG 5 cut(s) 699, 738, 792, 836, 848
BmeT110I CYCGRG 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 5 cut(s) 699, 738, 792, 836, 848
BmsI GCATC 2 cut(s) 556, 834
BpiI GAAGAC 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 694
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 4 cut(s) 246, 598, 698, 736
BsaXI ACNNNNNCTCC 6 cut(s) 212, 242, 276, 306, 663, 693
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 742
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 19, 711
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 5 cut(s) 699, 738, 792, 836, 848
BseDI CCNNGG 4 cut(s) 246, 598, 698, 736
BseGI GGATG 1 cut(s) 186
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 2 cut(s) 481, 742
BseNI ACTGG 2 cut(s) 19, 711
BseRI GAGGAG 2 cut(s) 370, 464
BseXI GCAGC 4 cut(s) 2, 115, 161, 472
BsgI GTGCAG 2 cut(s) 216, 393
BshFI GGCC 5 cut(s) 161, 191, 603, 795, 846
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 2 cut(s) 481, 742
BsmAI GTCTC 2 cut(s) 99, 788
BsmBI CGTCTC 1 cut(s) 99
BsnI GGCC 5 cut(s) 161, 191, 603, 795, 846
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 4 cut(s) 238, 464, 550, 606
Bsp19I CCATGG 1 cut(s) 246
BspANI GGCC 5 cut(s) 161, 191, 603, 795, 846
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 2 cut(s) 472, 558
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 2 cut(s) 19, 711
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 246, 598, 698, 736
BssMI GATC 4 cut(s) 238, 464, 550, 606
BssT1I CCWWGG 2 cut(s) 246, 598
Bst2UI CCWGG 5 cut(s) 699, 738, 792, 836, 848
Bst4CI ACNGT 4 cut(s) 93, 228, 394, 721
BstAPI GCANNNNNTGC 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 336, 427
BstDEI CTNAG 3 cut(s) 253, 688, 802
BstDSI CCRYGG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 479
BstF5I GGATG 1 cut(s) 186
BstKTI GATC 4 cut(s) 241, 467, 553, 609
BstMAI GTCTC 2 cut(s) 99, 788
BstMBI GATC 4 cut(s) 238, 464, 550, 606
BstMWI GCNNNNNNNGC 4 cut(s) 158, 407, 494, 831
BstNI CCWGG 5 cut(s) 699, 738, 792, 836, 848
BstSCI CCNGG 5 cut(s) 697, 736, 790, 834, 846
BstV1I GCAGC 4 cut(s) 2, 115, 161, 472
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 3 cut(s) 464, 550, 606
BstYI RGATCY 3 cut(s) 464, 550, 606
BsuRI GGCC 5 cut(s) 161, 191, 603, 795, 846
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 2 cut(s) 159, 204
BtsIMutI CAGTG 3 cut(s) 159, 204, 300
Cac8I GCNNGC 2 cut(s) 336, 427
Cfr10I RCCGGY 1 cut(s) 191
Csp6I GTAC 1 cut(s) 714
CviAII CATG 4 cut(s) 247, 430, 761, 787
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 3 cut(s) 253, 688, 802
DpnI GATC 4 cut(s) 240, 466, 552, 608
DpnII GATC 4 cut(s) 238, 464, 550, 606
DraIII CACNNNGTG 1 cut(s) 416
EaeI YGGCCR 3 cut(s) 159, 189, 601
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 246, 598
Eco147I AGGCCT 1 cut(s) 795
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 766
Eco53kI GAGCTC 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 479
EcoRII CCWGG 5 cut(s) 697, 736, 790, 834, 846
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 2 cut(s) 246, 598
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 246, 598
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 4 cut(s) 250, 433, 764, 790
FatI CATG 4 cut(s) 246, 429, 760, 786
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 16, 129, 150, 486
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 4 cut(s) 16, 129, 150, 486
FspBI CTAG 1 cut(s) 63
GluI GCNGC 4 cut(s) 16, 129, 150, 486
GsuI CTGGAG 1 cut(s) 694
HaeIII GGCC 5 cut(s) 161, 191, 603, 795, 846
HapII CCGG 1 cut(s) 192
Hin1II CATG 4 cut(s) 250, 433, 764, 790
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 3 cut(s) 203, 451, 530
HpaI GTTAAC 1 cut(s) 231
HpaII CCGG 1 cut(s) 192
HphI GGTGA 2 cut(s) 235, 674
Hpy166II GTNNAC 3 cut(s) 231, 724, 748
Hpy188I TCNGA 4 cut(s) 51, 565, 756, 812
Hpy188III TCNNGA 2 cut(s) 534, 829
Hpy8I GTNNAC 3 cut(s) 231, 724, 748
HpyAV CCTTC 3 cut(s) 78, 318, 704
HpyCH4III ACNGT 4 cut(s) 93, 228, 394, 721
HpyCH4IV ACGT 1 cut(s) 636
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 407, 494, 831
HpyF3I CTNAG 3 cut(s) 253, 688, 802
HpySE526I ACGT 1 cut(s) 636
Hsp92II CATG 4 cut(s) 250, 433, 764, 790
KspAI GTTAAC 1 cut(s) 231
Kzo9I GATC 4 cut(s) 238, 464, 550, 606
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 4 cut(s) 2, 115, 161, 472
LweI GCATC 2 cut(s) 556, 834
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 1 cut(s) 163
MalI GATC 4 cut(s) 240, 466, 552, 608
MboI GATC 4 cut(s) 238, 464, 550, 606
MboII GAAGA 4 cut(s) 203, 545, 632, 677
MflI RGATCY 3 cut(s) 464, 550, 606
MhlI GDGCHC 1 cut(s) 175
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 4 cut(s) 378, 489, 522, 576
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 1 cut(s) 445
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 8 cut(s) 138, 170, 178, 348, 485, 599, 718, 736
Mox20I TGGCCA 1 cut(s) 603
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 230, 381, 639
MslI CAYNNNNRTG 1 cut(s) 773
Msp20I TGGCCA 1 cut(s) 603
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 5 cut(s) 699, 738, 792, 836, 848
MvaI CCWGG 5 cut(s) 699, 738, 792, 836, 848
MwoI GCNNNNNNNGC 4 cut(s) 158, 407, 494, 831
NcoI CCATGG 1 cut(s) 246
NdeII GATC 4 cut(s) 238, 464, 550, 606
NlaIII CATG 4 cut(s) 250, 433, 764, 790
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 174
PceI AGGCCT 1 cut(s) 795
PfeI GAWTC 2 cut(s) 203, 530
PkrI GCNGC 4 cut(s) 17, 130, 151, 487
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 5 cut(s) 697, 736, 790, 834, 846
PspGI CCWGG 5 cut(s) 697, 736, 790, 834, 846
PspN4I GGNNCC 1 cut(s) 259
PspXI VCTCGAGB 1 cut(s) 174
PsuI RGATCY 3 cut(s) 464, 550, 606
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 1 cut(s) 175
SaqAI TTAA 3 cut(s) 230, 381, 639
SatI GCNGC 4 cut(s) 16, 129, 150, 486
Sau3AI GATC 4 cut(s) 238, 464, 550, 606
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 5 cut(s) 699, 738, 792, 836, 848
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 2 cut(s) 556, 834
Sfr274I CTCGAG 1 cut(s) 174
SlaI CTCGAG 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 174
SmoI CTYRAG 1 cut(s) 174
Sse9I AATT 4 cut(s) 378, 489, 522, 576
SseBI AGGCCT 1 cut(s) 795
SspMI CTAG 1 cut(s) 63
SstI GAGCTC 1 cut(s) 175
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 5 cut(s) 697, 736, 790, 834, 846
StyI CCWWGG 2 cut(s) 246, 598
TaaI ACNGT 4 cut(s) 93, 228, 394, 721
TaiI ACGT 1 cut(s) 639
TaqI TCGA 1 cut(s) 175
TasI AATT 4 cut(s) 378, 489, 522, 576
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 2 cut(s) 203, 530
Tru1I TTAA 3 cut(s) 230, 381, 639
Tru9I TTAA 3 cut(s) 230, 381, 639
TscAI CASTG 3 cut(s) 159, 204, 307
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 4 cut(s) 15, 128, 149, 485
Tsp45I GTSAC 1 cut(s) 163
TspGWI ACGGA 1 cut(s) 563
TspRI CASTG 3 cut(s) 159, 204, 307
XagI CCTNNNNNAGG 1 cut(s) 479
XhoI CTCGAG 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.