AT5G52910

timeless

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007218
Physical Location & Seq
Reverse (-)
21457534 .. 21463327
5794 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52910.1

Sequence Viewer

Length: 3426 bp
ATGGATGTGGAGGAGAGAATCTCAACGAAGAAGAAGATAGATTTGGAAGGATTATCAGTAATCTGCAGCGATCTGGGTCTTCCCGAGGAAGACGAAGACAGAAGAAGAATTGGGTACTCGAAAAGCGAGTACTGTCTCGACAATTTGAAAGATTTACTGAGATTTCTGAGACGCGACGATCCTGAGAGCAGAGAAGTTTTCAAACAAGTCTGTGCGTGGAATATAGTCTCTAAAGATTTGATTCCGATCATTGAGCATTACCAAGATGAACATAACTTAGTTTTAAATGCAGTTAAAGTGTTGGTGTTTCTTACAATGCCTATAGAGCCTTCTTCAGATGATATTCCTCAGCAGTTAGAGTATCTATGGGGATTGAAATCTGCAATTACTTTTAGTAATATTGTTGCAGTGATTGTGTCTTTGCTTGAAGCTCCTCTTGAGAACTTAGAATTAGATGTATTCAATGAGGAAGATTGGAAATTGGTTCAGCTTGTGCTTACTTTATTCAGGAATTTACTTGCTATTCATGATGTATCTCCGATTCAGAAAGCTGGAGAGTCTACTTGTTACTTTTTGTCGTTGAGGGATCAGTTTCTCGAAGTTCTGTCTCGTGAGAATGTGATGGATATAGTTCTTGTGATAACTCAGACTATTGAGGGTTTCAATAGTTTGTTGAGGCATGACAACTTACTTCTGCTTGAGATTTATCATTATATTCTCTTGGGTCAAGACATGGAGCTCGTTGCAAAGGCTCCTGAGAAGTTGGATCAGGGGAAACAAGCTTCTGTAGATAGTTTGAAGACATTAATGAAGGAGGAGGAAGTTAAAAGAAAGCTTGCAAGACTCAATAATATGAATCAGCGTCATTCACAATTTGGTGGGACATTCACCCGGGTTACCATGGATGGCACTAAAGCTGTACTAAAGGGAATACCTTCTACTACGGAAAGCACAATGCTTAAGCCTCAGCAAGGTCGTGGAGCCACCGAAAAGATTGTCTGGGAACATGGACCAATGTCTGTGACAAATGACAAATCATTATGTGAAGATATTGAAAAGGAGCATCCCTCCATTCAAAATAGCGACATTGTTACTTTCTTCCAAGTTGCTCAGTCCATCACCTCTTTTCAGTTTCACAAGTCTTCGGCTTCTAGTCCAGCAATAGAAACAGAGGAAACCTCTGAACTCACTACCAACCAGAAGGCTGGAGTAAACTTTTCCAAGAGTGACATTTGTGCGCCGATTGCAGCAACAATTAACGACAGAATGTTTTTGCTTGTCATTTCAAAATGGCGCTGTGCATTTGATGGTCTGAAAGAAACTAAAGATTTTAAGTTTCTATCTGCAGCAAGCTCTCTTGTGAAAACCATGCTTTGCTTACTAGATCTGGTGATCAAATTGCTACCTGAAGACTCTAAGGAAGCTTTTACTGTCCGCATCTTGCTGTATAAGTTGTTTTATGACCAGACTGATCAAGGGATGTGTCAGTTCATCCTGAATCTGGTGAGAAGTTTTGACACGCACAAGCAACCTAAGAGTGAGCTTGGAGATCTGGTGGAAAGCATCCATATAATTGTAGGCCTTATGGAAAACCTTCAAGGGCGTGGAACTTTGAGGGTTTCAAAAAAATCAAGGAAAGCAAGAAAGAAAAAACCCAAGGGAAATAAGGAGGCCACAGTGCATAAACTGTCTGAAAATCATCCAAGTACTTCAAACGAAGCTAGCACAGCAAAAAGCATACCGATGGTTGATTCAACAGTTTCAACAGAGGATGGACCAATGGATGTGCCTCCCAATAAACCAGAGGCATCGAACCTAGAGACTGAAACTGATGAAACTCAACAAATGCACAGCCCGAAGTCAAATAATGTGGTTGATGATCTGTCTAGCGGCAGTGATGATTCTTCTGATGGCGAAGAGCAAACTGCAACAGATGAAGTTGATTTTAAGGTTTCCACATTTATTTCTGCTTTTGCCAGCAACAGCATTATTCAAAACCTATGCTGGTTGCTTAAGTTTTACAAAAGCAATCCAAAACAGACGAATCACCACGTAATAAGCATTTTACGAAGGATTACAGAAGACTTGGAGCTCGCTCCCATGTTGTATCAGCTGTCACTTCTAATAACATTCCATAAGATCCTTGATGAGCAGAAAGTTTGTCCCTGCAAAGACTATGAGAACATAGTTACCTTCCTGACAGATCTGGTTAGAAATATGTTGAAGAAAATGAAGTCACAGCCTCTTCTCTTTGTGGAAATTCTCTTCTCAAAGACTCGCAAAGAATGCCATTACATAAATGCTGAATATATGTTGCATGAGCTTGGCCATCTAAGAAAACAAATGGGTAATCAAGAAAAGTTCTCAGGAACTGAGGAATTTGGTACATCATCAGACAAAGGATGGGCTCACAGAAGCTTAGCAGATGCTCTAGGTGATGATGAAGCTGATGTCGTAATTTCTTATGATCAAGGATTTCAAAATGAGGATGATGACATGGTGGAAGATAAATCTGCAGGACCATCTAAAAGAAAGCGAAGACTTGTTCTTGATGGTGATATGGATATAAAAATTAAAGACCTATATGACAGGTACAAAGATGATAAAAATTGCAGCCGGCTTATTGCTGAAAATTTGGTTCCTGATGGTGGAATTTCTGCTGCACAAGTAACCAACAAGCTTAAACAGCTAGGTCTAGAAACAAGGAAAAGGCTTCGGCGTGGAGGTACTGATCACCTGGATGCAACATCGTTAGCACAACCTTCAAACACCAGAAAGAGAGTGAGCTCCTTTAGCAAAGAACAAGAAACGCTTATTAAGGAATTATACAAGAAGTTCAAGGACGAAAAGCGCTGTTGTTATTTGATTGCCAGTGAATTGGGCTCTGAAAATACATATACTACTGCCCAGGTTTCACGTAAATTAAAGCAACTTGGTCTCCGTCTTCCCCGTGGTAAGAAGTCTGAAGCTGGCATGATGCTGAAAGATGACCATGATGATTCTTCAGCAGATGAATCCGAGGATGAAACATTACTAGCCTTCAAGAACAGGAAGAGCAGGAAAAATCAGAAGAACAAGCAGCAAACAGGATCAATCAATGAGATCACTCCAGGAGGTTCTGAAGACAACACAGAAAGGAATGAAACTAGTCTGCATGAGCCTACCGCTGGGGAAGAAGAGGACAATGATCAAAGCTACATCACCAGGGAAAGTAGACAATCTGAAACAGACGTTCACATCAGCGACAATGGTCCTTCAACTTCATTGCCAGAAGATCCAAACCTTTCATCAGATAATGAACTAGAGGATGACGAGTTAGCAGATTGGGGTGATGACTCTGCAGCTGGTGGTGCCTCCCTCACTCAAAGCCCGCTTAGTAGAAGGAAACTGAAGATGGTACTTGATAAGGACGATGAGGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1141

Amino Acids

128.9

Weight (kDa)

5.17

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 39 - 297 1.9e-61 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 3350
AccI GTMKAC 2 cut(s) 560, 3214
AccII CGCG 1 cut(s) 174
AciI CCGC 4 cut(s) 1435, 1890, 3165, 3371
AclWI GGATC 6 cut(s) 173, 594, 774, 2134, 3097, 3269
AcoI YGGCCR 1 cut(s) 2326
AcsI RAATTY 5 cut(s) 511, 2259, 2378, 2632, 2652
AcuI CTGAAG 6 cut(s) 318, 1428, 2985, 2988, 3141, 3410
AfaI GTAC 8 cut(s) 116, 131, 921, 1708, 2386, 2594, 2728, 3399
AfeI AGCGCT 1 cut(s) 2852
AfiI CCNNNNNNNGG 4 cut(s) 971, 1501, 2648, 3167
AflII CTTAAG 2 cut(s) 959, 2012
AhlI ACTAGT 1 cut(s) 3146
AjnI CCWGG 4 cut(s) 2736, 2907, 3109, 3203
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
Alw21I GWGCWC 3 cut(s) 741, 2094, 2789
Alw26I GTCTC 6 cut(s) 140, 163, 232, 612, 1814, 2942
AlwI GGATC 6 cut(s) 173, 594, 774, 2134, 3097, 3269
AlwNI CAGNNNCTG 1 cut(s) 2372
Ama87I CYCGRG 2 cut(s) 83, 891
Aor51HI AGCGCT 1 cut(s) 2852
AoxI GGCC 3 cut(s) 1579, 1671, 2326
ApeKI GCWGC 7 cut(s) 66, 1247, 1346, 2613, 2660, 3079, 3341
ApoI RAATTY 5 cut(s) 511, 2259, 2378, 2632, 2652
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
AseI ATTAAT 1 cut(s) 806
Asp700I GAANNNNTTC 2 cut(s) 934, 1593
AspLEI GCGC 3 cut(s) 1240, 1296, 2853
AspS9I GGNCC 4 cut(s) 1010, 1775, 2519, 3251
AsuC2I CCSGG 2 cut(s) 892, 893
AsuNHI GCTAGC 1 cut(s) 1721
AvaI CYCGRG 2 cut(s) 83, 891
AvaII GGWCC 4 cut(s) 1010, 1775, 2519, 3251
BalI TGGCCA 1 cut(s) 2328
BanI GGYRCC 1 cut(s) 3350
BanII GRGCYC 5 cut(s) 741, 2094, 2410, 2789, 2885
BauI CACGAG 1 cut(s) 609
Bbv12I GWGCWC 3 cut(s) 741, 2094, 2789
BbvCI CCTCAGC 2 cut(s) 348, 966
BbvI GCAGC 7 cut(s) 78, 1259, 1358, 2625, 2647, 3091, 3353
BciT130I CCWGG 4 cut(s) 2738, 2909, 3111, 3205
BclI TGATCA 5 cut(s) 1392, 1471, 2467, 2731, 3187
BcnI CCSGG 2 cut(s) 892, 893
BcoDI GTCTC 6 cut(s) 140, 163, 232, 612, 1814, 2942
BcuI ACTAGT 1 cut(s) 3146
BfmI CTRYAG 6 cut(s) 64, 321, 786, 1344, 2514, 3339
BfoI RGCGCY 2 cut(s) 1297, 2854
BfrI CTTAAG 2 cut(s) 959, 2012
BglII AGATCT 3 cut(s) 1384, 1549, 2203
BisI GCNGC 8 cut(s) 67, 1248, 1347, 1891, 2614, 2661, 3080, 3342
BlpI GCTNAGC 1 cut(s) 2419
BlsI GCNGC 8 cut(s) 68, 1249, 1348, 1892, 2615, 2662, 3081, 3343
BmcAI AGTACT 2 cut(s) 131, 1708
Bme1390I CCNGG 6 cut(s) 892, 893, 2738, 2909, 3111, 3205
Bme18I GGWCC 4 cut(s) 1010, 1775, 2519, 3251
BmeT110I CYCGRG 2 cut(s) 83, 891
BmgT120I GGNCC 4 cut(s) 1010, 1775, 2519, 3251
BmiI GGNNCC 4 cut(s) 753, 982, 2640, 3352
BmrFI CCNGG 6 cut(s) 892, 893, 2738, 2909, 3111, 3205
BmsI GCATC 7 cut(s) 1072, 1446, 1572, 1817, 2416, 2731, 2967
BmtI GCTAGC 1 cut(s) 1725
BoxI GACNNNNGTC 2 cut(s) 1015, 3249
BplI GAGNNNNNCTC 5 cut(s) 37, 1052, 1084, 1163, 1195
BpmI CTGGAG 3 cut(s) 573, 1227, 3093
Bpu10I CCTNAGC 2 cut(s) 348, 966
Bpu1102I GCTNAGC 1 cut(s) 2419
BpuEI CTTGAG 2 cut(s) 458, 719
BpuMI CCSGG 2 cut(s) 892, 893
BsaAI YACGTR 2 cut(s) 2053, 2918
BsaBI GATNNNNATC 2 cut(s) 245, 376
BsaI GGTCTC 1 cut(s) 2942
BsaJI CCNNGG 8 cut(s) 84, 891, 900, 1656, 2907, 2950, 3018, 3204
BsaXI ACNNNNNCTCC 4 cut(s) 1539, 1569, 2922, 2952
Bsc4I CCNNNNNNNGG 4 cut(s) 971, 1501, 2648, 3167
Bse118I RCCGGY 1 cut(s) 2616
Bse1I ACTGG 1 cut(s) 2871
Bse3DI GCAATG 1 cut(s) 3263
Bse8I GATNNNNATC 2 cut(s) 245, 376
BseBI CCWGG 4 cut(s) 2738, 2909, 3111, 3205
BseDI CCNNGG 8 cut(s) 84, 891, 900, 1656, 2907, 2950, 3018, 3204
BseJI GATNNNNATC 2 cut(s) 245, 376
BseLI CCNNNNNNNGG 4 cut(s) 971, 1501, 2648, 3167
BseMI GCAATG 1 cut(s) 3263
BseNI ACTGG 1 cut(s) 2871
BseRI GAGGAG 3 cut(s) 26, 423, 830
BseXI GCAGC 7 cut(s) 78, 1259, 1358, 2625, 2647, 3091, 3353
BseYI CCCAGC 1 cut(s) 3167
BsgI GTGCAG 1 cut(s) 2646
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 3 cut(s) 1581, 1673, 2328
BshNI GGYRCC 1 cut(s) 3350
BsiHKAI GWGCWC 3 cut(s) 741, 2094, 2789
BsiHKCI CYCGRG 2 cut(s) 83, 891
BsiSI CCGG 2 cut(s) 892, 2617
BslFI GGGAC 2 cut(s) 895, 2148
BslI CCNNNNNNNGG 4 cut(s) 971, 1501, 2648, 3167
BsmAI GTCTC 6 cut(s) 140, 163, 232, 612, 1814, 2942
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 895, 2148
BsmI GAATGC 1 cut(s) 2291
BsnI GGCC 3 cut(s) 1581, 1673, 2328
Bso31I GGTCTC 1 cut(s) 2942
BsoBI CYCGRG 2 cut(s) 83, 891
Bsp1286I GDGCHC 5 cut(s) 741, 2094, 2410, 2789, 2885
Bsp1720I GCTNAGC 1 cut(s) 2419
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 4 cut(s) 1435, 1890, 3165, 3371
BspANI GGCC 3 cut(s) 1581, 1673, 2328
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 4 cut(s) 753, 982, 2640, 3352
BspMAI CTGCAG 4 cut(s) 68, 1348, 2518, 3343
BspOI GCTAGC 1 cut(s) 1725
BspPI GGATC 6 cut(s) 173, 594, 774, 2134, 3097, 3269
BspQI GCTCTTC 2 cut(s) 1911, 3047
BspT107I GGYRCC 1 cut(s) 3350
BspTI CTTAAG 2 cut(s) 959, 2012
BspTNI GGTCTC 1 cut(s) 2942
BsrDI GCAATG 1 cut(s) 3263
BsrFI RCCGGY 1 cut(s) 2616
BsrI ACTGG 1 cut(s) 2871
BssAI RCCGGY 1 cut(s) 2616
BssECI CCNNGG 8 cut(s) 84, 891, 900, 1656, 2907, 2950, 3018, 3204
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 2 cut(s) 900, 1656
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 4 cut(s) 2738, 2909, 3111, 3205
Bst4CI ACNGT 5 cut(s) 134, 1432, 1678, 1689, 1759
Bst6I CTCTTC 5 cut(s) 1911, 2250, 2270, 3047, 3171
BstAFI CTTAAG 2 cut(s) 959, 2012
BstAPI GCANNNNNTGC 1 cut(s) 2286
BstBAI YACGTR 2 cut(s) 2053, 2918
BstC8I GCNNGC 8 cut(s) 837, 1351, 1723, 1978, 2094, 2618, 2971, 3371
BstDSI CCRYGG 2 cut(s) 900, 2950
BstEII GGTNACC 1 cut(s) 895
BstENI CCTNNNNNAGG 1 cut(s) 969
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 2 cut(s) 1297, 2854
BstHHI GCGC 3 cut(s) 1240, 1296, 2853
BstMAI GTCTC 6 cut(s) 140, 163, 232, 612, 1814, 2942
BstMWI GCNNNNNNNGC 7 cut(s) 325, 1244, 1727, 2286, 2686, 2793, 3350
BstNI CCWGG 4 cut(s) 2738, 2909, 3111, 3205
BstPAI GACNNNNGTC 2 cut(s) 1015, 3249
BstPI GGTNACC 1 cut(s) 895
BstSCI CCNGG 6 cut(s) 890, 891, 2736, 2907, 3109, 3203
BstSFI CTRYAG 6 cut(s) 64, 321, 786, 1344, 2514, 3339
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 7 cut(s) 78, 1259, 1358, 2625, 2647, 3091, 3353
BstX2I RGATCY 5 cut(s) 1384, 1549, 2139, 2203, 3274
BstXI CCANNNNNNTGG 2 cut(s) 1205, 2878
BstYI RGATCY 5 cut(s) 1384, 1549, 2139, 2203, 3274
BsuRI GGCC 3 cut(s) 1581, 1673, 2328
BtgI CCRYGG 2 cut(s) 900, 2950
BtsI GCAGTG 2 cut(s) 414, 1900
BtsIMutI CAGTG 4 cut(s) 414, 1683, 1900, 2878
Cac8I GCNNGC 8 cut(s) 837, 1351, 1723, 1978, 2094, 2618, 2971, 3371
CaiI CAGNNNCTG 1 cut(s) 2372
CciI TCATGA 1 cut(s) 526
CfoI GCGC 3 cut(s) 1240, 1296, 2853
Cfr10I RCCGGY 1 cut(s) 2616
Cfr13I GGNCC 4 cut(s) 1010, 1775, 2519, 3251
Cfr9I CCCGGG 1 cut(s) 891
CseI GACGC 2 cut(s) 180, 851
Csp6I GTAC 8 cut(s) 115, 130, 920, 1707, 2385, 2593, 2727, 3398
CspCI CAANNNNNGTGG 2 cut(s) 1851, 1886
CviQI GTAC 8 cut(s) 115, 130, 920, 1707, 2385, 2593, 2727, 3398
DraI TTTAAA 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 2326
Eam1104I CTCTTC 5 cut(s) 1911, 2250, 2270, 3047, 3171
EarI CTCTTC 5 cut(s) 1911, 2250, 2270, 3047, 3171
Ecl136II GAGCTC 3 cut(s) 739, 2092, 2787
Eco130I CCWWGG 2 cut(s) 900, 1656
Eco147I AGGCCT 1 cut(s) 1581
Eco24I GRGCYC 5 cut(s) 741, 2094, 2410, 2789, 2885
Eco31I GGTCTC 1 cut(s) 2942
Eco47I GGWCC 4 cut(s) 1010, 1775, 2519, 3251
Eco47III AGCGCT 1 cut(s) 2852
Eco53kI GAGCTC 3 cut(s) 739, 2092, 2787
Eco57I CTGAAG 6 cut(s) 318, 1428, 2985, 2988, 3141, 3410
Eco88I CYCGRG 2 cut(s) 83, 891
Eco91I GGTNACC 1 cut(s) 895
EcoICRI GAGCTC 3 cut(s) 739, 2092, 2787
EcoNI CCTNNNNNAGG 1 cut(s) 969
EcoO65I GGTNACC 1 cut(s) 895
EcoRII CCWGG 4 cut(s) 2736, 2907, 3109, 3203
EcoT14I CCWWGG 2 cut(s) 900, 1656
EcoT38I GRGCYC 5 cut(s) 741, 2094, 2410, 2789, 2885
ErhI CCWWGG 2 cut(s) 900, 1656
Esp3I CGTCTC 1 cut(s) 163
FalI AAGNNNNNCTT 4 cut(s) 420, 452, 2796, 2828
FaqI GGGAC 2 cut(s) 895, 2148
FauI CCCGC 1 cut(s) 3378
FbaI TGATCA 5 cut(s) 1392, 1471, 2467, 2731, 3187
FblI GTMKAC 2 cut(s) 560, 3214
Fnu4HI GCNGC 8 cut(s) 67, 1248, 1347, 1891, 2614, 2661, 3080, 3342
FriOI GRGCYC 5 cut(s) 741, 2094, 2410, 2789, 2885
Fsp4HI GCNGC 8 cut(s) 67, 1248, 1347, 1891, 2614, 2661, 3080, 3342
GlaI GCGC 3 cut(s) 1239, 1295, 2852
GluI GCNGC 8 cut(s) 67, 1248, 1347, 1891, 2614, 2661, 3080, 3342
GsaI CCCAGC 1 cut(s) 3171
GsuI CTGGAG 3 cut(s) 573, 1227, 3093
HaeII RGCGCY 2 cut(s) 1297, 2854
HaeIII GGCC 3 cut(s) 1581, 1673, 2328
HapII CCGG 2 cut(s) 892, 2617
HgaI GACGC 2 cut(s) 180, 851
HhaI GCGC 3 cut(s) 1240, 1296, 2853
Hin6I GCGC 3 cut(s) 1238, 1294, 2851
HinP1I GCGC 3 cut(s) 1238, 1294, 2851
HindIII AAGCTT 5 cut(s) 780, 833, 1422, 2416, 2678
HpaII CCGG 2 cut(s) 892, 2617
Hpy166II GTNNAC 4 cut(s) 561, 1213, 3215, 3235
Hpy8I GTNNAC 4 cut(s) 561, 1213, 3215, 3235
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 5 cut(s) 134, 1432, 1678, 1689, 1759
HpyCH4IV ACGT 3 cut(s) 2052, 2917, 3231
HpyF10VI GCNNNNNNNGC 7 cut(s) 325, 1244, 1727, 2286, 2686, 2793, 3350
HpySE526I ACGT 3 cut(s) 2052, 2917, 3231
HspAI GCGC 3 cut(s) 1238, 1294, 2851
KroI GCCGGC 1 cut(s) 2616
KroNI GCCGGC 1 cut(s) 2618
Ksp22I TGATCA 5 cut(s) 1392, 1471, 2467, 2731, 3187
LguI GCTCTTC 2 cut(s) 1911, 3047
LmnI GCTCC 8 cut(s) 436, 736, 757, 980, 1060, 2089, 2101, 2792
Lsp1109I GCAGC 7 cut(s) 78, 1259, 1358, 2625, 2647, 3091, 3353
LweI GCATC 7 cut(s) 1072, 1446, 1572, 1817, 2416, 2731, 2967
MaeII ACGT 3 cut(s) 2052, 2917, 3231
MaeIII GTNAC 9 cut(s) 566, 895, 1021, 1090, 1226, 2115, 2188, 2235, 2668
MflI RGATCY 5 cut(s) 1384, 1549, 2139, 2203, 3274
MhlI GDGCHC 5 cut(s) 741, 2094, 2410, 2789, 2885
MlsI TGGCCA 1 cut(s) 2328
MluNI TGGCCA 1 cut(s) 2328
MlyI GAGTC 5 cut(s) 566, 837, 1406, 2269, 3329
MmeI TCCRAC 1 cut(s) 744
Mox20I TGGCCA 1 cut(s) 2328
MroNI GCCGGC 1 cut(s) 2616
MroXI GAANNNNTTC 2 cut(s) 934, 1593
MscI TGGCCA 1 cut(s) 2328
MslI CAYNNNNRTG 2 cut(s) 1742, 2739
Msp20I TGGCCA 1 cut(s) 2328
MspA1I CMGCKG 3 cut(s) 2113, 3167, 3344
MspCI CTTAAG 2 cut(s) 959, 2012
MspI CCGG 2 cut(s) 892, 2617
MspR9I CCNGG 6 cut(s) 892, 893, 2738, 2909, 3111, 3205
Mva1269I GAATGC 1 cut(s) 2291
MvaI CCWGG 4 cut(s) 2738, 2909, 3111, 3205
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 7 cut(s) 325, 1244, 1727, 2286, 2686, 2793, 3350
NaeI GCCGGC 1 cut(s) 2618
NciI CCSGG 2 cut(s) 892, 893
NcoI CCATGG 1 cut(s) 900
NgoMIV GCCGGC 1 cut(s) 2616
NheI GCTAGC 1 cut(s) 1721
NlaIV GGNNCC 4 cut(s) 753, 982, 2640, 3352
NmuCI GTSAC 4 cut(s) 1021, 1226, 2115, 2235
PagI TCATGA 1 cut(s) 526
PceI AGGCCT 1 cut(s) 1581
PciSI GCTCTTC 2 cut(s) 1911, 3047
PctI GAATGC 1 cut(s) 2291
PdiI GCCGGC 1 cut(s) 2618
PdmI GAANNNNTTC 2 cut(s) 934, 1593
PfoI TCCNGGA 1 cut(s) 3109
PkrI GCNGC 8 cut(s) 68, 1249, 1348, 1892, 2615, 2662, 3081, 3343
PleI GAGTC 5 cut(s) 565, 837, 1406, 2269, 3329
PpsI GAGTC 5 cut(s) 565, 837, 1406, 2269, 3329
Ppu21I YACGTR 2 cut(s) 2053, 2918
PshAI GACNNNNGTC 2 cut(s) 1015, 3249
PshBI ATTAAT 1 cut(s) 806
Psp124BI GAGCTC 3 cut(s) 741, 2094, 2789
Psp6I CCWGG 4 cut(s) 2736, 2907, 3109, 3203
PspEI GGTNACC 1 cut(s) 895
PspFI CCCAGC 1 cut(s) 3167
PspGI CCWGG 4 cut(s) 2736, 2907, 3109, 3203
PspN4I GGNNCC 4 cut(s) 753, 982, 2640, 3352
PspPI GGNCC 4 cut(s) 1010, 1775, 2519, 3251
PstI CTGCAG 4 cut(s) 68, 1348, 2518, 3343
PstNI CAGNNNCTG 1 cut(s) 2372
PsuI RGATCY 5 cut(s) 1384, 1549, 2139, 2203, 3274
PvuII CAGCTG 2 cut(s) 2113, 3344
RsaI GTAC 8 cut(s) 116, 131, 921, 1708, 2386, 2594, 2728, 3399
RsaNI GTAC 8 cut(s) 115, 130, 920, 1707, 2385, 2593, 2727, 3398
RseI CAYNNNNRTG 2 cut(s) 1742, 2739
SacI GAGCTC 3 cut(s) 741, 2094, 2789
SapI GCTCTTC 2 cut(s) 1911, 3047
SatI GCNGC 8 cut(s) 67, 1248, 1347, 1891, 2614, 2661, 3080, 3342
Sau96I GGNCC 4 cut(s) 1010, 1775, 2519, 3251
ScaI AGTACT 2 cut(s) 131, 1708
SchI GAGTC 5 cut(s) 566, 837, 1406, 2269, 3329
ScrFI CCNGG 6 cut(s) 892, 893, 2738, 2909, 3111, 3205
SduI GDGCHC 5 cut(s) 741, 2094, 2410, 2789, 2885
SfaNI GCATC 7 cut(s) 1072, 1446, 1572, 1817, 2416, 2731, 2967
SfcI CTRYAG 6 cut(s) 64, 321, 786, 1344, 2514, 3339
SinI GGWCC 4 cut(s) 1010, 1775, 2519, 3251
SmaI CCCGGG 1 cut(s) 893
SmiMI CAYNNNNRTG 2 cut(s) 1742, 2739
SmlI CTYRAG 4 cut(s) 437, 698, 959, 2012
SmoI CTYRAG 4 cut(s) 437, 698, 959, 2012
SpeI ACTAGT 1 cut(s) 3146
SseBI AGGCCT 1 cut(s) 1581
SsiI CCGC 4 cut(s) 1435, 1890, 3165, 3371
SspI AATATT 1 cut(s) 400
SstI GAGCTC 3 cut(s) 741, 2094, 2789
StuI AGGCCT 1 cut(s) 1581
StyD4I CCNGG 6 cut(s) 890, 891, 2736, 2907, 3109, 3203
StyI CCWWGG 2 cut(s) 900, 1656
TaaI ACNGT 5 cut(s) 134, 1432, 1678, 1689, 1759
TaiI ACGT 3 cut(s) 2055, 2920, 3234
TaqI TCGA 4 cut(s) 119, 138, 597, 1811
TatI WGTACW 3 cut(s) 129, 919, 1706
TauI GCSGC 1 cut(s) 1893
TscAI CASTG 4 cut(s) 414, 1683, 1900, 2878
TseFI GTSAC 4 cut(s) 1021, 1226, 2115, 2235
TseI GCWGC 7 cut(s) 66, 1247, 1346, 2613, 2660, 3079, 3341
Tsp45I GTSAC 4 cut(s) 1021, 1226, 2115, 2235
TspGWI ACGGA 2 cut(s) 959, 2930
TspMI CCCGGG 1 cut(s) 891
TspRI CASTG 4 cut(s) 414, 1683, 1900, 2878
Vha464I CTTAAG 2 cut(s) 959, 2012
VpaK11BI GGWCC 4 cut(s) 1010, 1775, 2519, 3251
VspI ATTAAT 1 cut(s) 806
XagI CCTNNNNNAGG 1 cut(s) 969
XapI RAATTY 5 cut(s) 511, 2259, 2378, 2632, 2652
XbaI TCTAGA 1 cut(s) 2695
XmaI CCCGGG 1 cut(s) 891
XmiI GTMKAC 2 cut(s) 560, 3214
XmnI GAANNNNTTC 2 cut(s) 934, 1593
ZrmI AGTACT 2 cut(s) 131, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.