RchiOBHm_Chr1g0379691

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007218
Physical Location & Seq
Reverse (-)
65676687 .. 65685900
9214 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60300

Sequence Viewer

Length: 3765 bp
ATGGATTTGCAAGGCTTATCGGTGATCTGCAACGGCCTCGGAATCGCCCTGGACGACGACGACGGAAACCGGATCGGTTACAGGAAGCACAAGTACTGCTTAGATAATTTGAAGGATTTGCTGAGGTTCTTGAGGCGCGATGATCCGCAGAACCGGGATGTTTTCAAGGAAGTATGCAAGTGGAATATTGTATCCAAGGAGCTGATACCTATTATTGAGCACTGCCAAGATGATCGCAGCTTGGTTTTAAATGCAGTGAAGGTTCTGGTGTTCCTGACTATGCCAGTGGAGGCTACATCAAATGACATTTTACAACAAATTGAATTTCTATGGGAGCTGAAGTCTTCAATTACGTCTAGTGACATTGTTGCGGTTATAGTTTCACTTCTGGAAAGTCCACTAGAAAATCTGGAAAGTGATGTGTTCACAGAGGACGATTGGAAATTGGTACAGCTGGTACTTACTCTATTCCGAAACATTTTAGCCATTCAAGACATTTCATTGCAGCAGAAGTCTGGGGGTACTGCCAGTCAATTTCTATCACGCAGAGATAGCTTTCTGGAACTTTTGTTTCGTGAAAATGTGATGGACTTGGTTTTAGCCATAACACAGCATATTGGTGATCATCGTAGTTATCTCTCTCATGATAACTTGCTTCTATTGGAAATTTTCCATTATACATTTATAGGGCAAGAGCCAGAGTTGATTGCAAATGCATATTCAAAAGGTGCTAAGGTGGAAGAAGATACTATAGATTCTGTAAATAGTCTCAAGTCTATTATGGAGGAAGAGAAAGAGAAAAAGAGACTAAAAGTTAGTCGTCATTCCAATTTTGGTGGGACATTTATTCGGCTTACCATGGATGGTTCAAAGGAAGTTGTCAAGGGAAGACCTACTTCTGCTTCTTGTAATACCCTACAGAAATCACAAAATCGTCGAGGTCCAATAAAAAAGATTGCTTGGGATCATGGGATATTACCTTCAACAGAGGATAAGATTTTGGAATTGCTTCATGATTTTGTTAACCAGTTTTTATCAGGGGGTTACAATGTTTTAATGCAGTCAATTCGCCAGGCTATTGAAAAGGAACACCACTCAATCCAGAAGAGTGATGTCATTATCTTCTTTCAGGTTGCTCAGTTTGTTACTTCTTGCCAATATCACAGGCATTCAATTTCTAAGCCAAGCCTGAGCGCTGAAGCAGACACAATTGAAGTTCCCACTGGTAAAGAGGCTGATAGAACATTCTTTAGAGGTGATATATGTGGACCCATTGCAACCTCAATGAATGAGTCGATGTTCCAACTAGTCATTTCAAAGTGGCGTTATGCATTCGATGGGTTGAAGGAAACACATGACTACAAGTTTCTGTCTGCAGCTGGTTCTCTTATGAAAGAAATGATTCGCATGCTAGATTTGGTGCTTAAGTTATTGCCAGAAGACTCCAAGGAGCCTCAAACAGCTCGCATACTTCTGTACAAGTTATTTTATGATCAGACCGAGGAGGGGATGACCCATTTTCTCTTAGGGTTGCTGAAATCGTTTGACACTCACAAACAACCAAGAAGTGATCTTGCAGATCTGGTAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCATCTGGAACATTGAGGGTTTCTAAGAAGTCAAGGAAGGGGAGGAAGAGGAGAAAACTGAGTGAAAAGGAAACAGAAAATAAGCTTTCCGGAGAGCATGATACGGTTCAGAAAGAGACTGACATATCCAATGGTGAACAATTAACAGATATGAGTGTAACCGAGAAAAGAAGCCTAGACACAAGTTCTAATGAGAAACAAGACATCAGCATCCCTGGCCAACCTGATGAAGGCAAAAACTCTTTGTTGAAGACAGAAAACTTCTCGAGCAGTCAGGCACAGTTAGATCACAAAAATTCTGGTGATGCAAAAGGTGATCTTTGTTATAGTACAGGTGACTCTTCTGGTGATGAGGAAGTAGCTGCAACTACTGAAGTCGATTTTAATGTGTCAAGTTTGATTTCTGCTTTTTCAAACAACAGCATCATCCAGAAATTGTGTTGGTTGCTTAAATTCTATAAGAGTAACTTGACCAGAACAAACCACTACATCGTATGCATGTTGAGAAGGATCAGTGAAGACCTGGAGCTCTCTCCAATGCTGTATCAGTTATCACTCCTCACTATATTCTATGATATCCTAGCTGAGCAGAATTCATCTCCTTGCAATGAATATGAAAACATTGTTGGTTTCTTGAAAAGTTTGGTCAGGAAAATGCTAAAAAAAATGAAACATCAACCCTTACTTTTTGTGGAAATTCTCTTCTGGAAGACTCGCAAAGAGTGTCATTACATTAATGCTGAATATTTATTGCATGAGCTTGGCCAATTGAGGAATGAAAGTAGAAACTGGGGAGATACTTTAGAGGATGGAGATATGGGTCGATCAATGGACAAGGGATGGACAGCTAGAAGTCTAGCAGATGCACTTGGTGAAGATGAAGCTGATGTTGTGCTCGACTTTGGACATAAGAACAATAAAGAAAATCTTGGAAAAGTGAAAGGCGATGCTGCATCTACTTCAGATAATGAGATTGATGAAAGCGGGGGCCAGTCCATGAAAACTGAAACTGGAAGGGGTCCTCTAAAAAAGAAAAGACTAGTTATTGGAGAGGAACAGGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGAGAACCACAATTGTAGTCTACTAATTGCTGAAGCTCTTGATCCTGATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAACAGCTTGGACTCAAAGTTGCACGAAGGAGAAGATTGCGCCATGCACAGGAATCTGTTTCTGCTGAGCCTAGTCAAACTGATGGAGATGGAGAAGTAGAGGAAACAGTTATTACTCATCATGAATCAAAAACTCGGCCTTTGCACACAAGAAAAAGAGTCCGTGGCTTAAGCGAAGCACAGGAAGCTCGAATTAGATCTCTCTATGATCAGTTTAAAGACCATAAAAGTTGCAACCACATGATTGCCAATGCAATGGATGAGGATGATAAATTGACAGCTCCTCAAGTTTCTCGTAAACTTAAGCAGCTTGGGTTATGTATTCCTCAGAGGAAGAGGTCTGGAGCTAACATGCCGATGAGGGATCAGGACCTAAATGATTCTGACACAAACAATGCCAATGCCTCTGATGATGAAACATTGTTATCATTAATGAACAGGGGCAAAAAGGATAATATCGGAATTAGTGAGGTACCAGAACAGATTACCAGAAGAGAAGTGTCAGAAGAGGAGTCTGATGATGAAATGCTAAGCTCTGTTCTTAAGAAAGCTAAGGGATCCCTTTCTAAATTAAAGGATCAAAACCTCACAGCCATATCGATTCAGGGAACAGCAAGAGGAAATGCTTTAGAAAATAAAGTTGAAGAAGCTGTTGTATCTTCTGGCAGGGATGTTAACGAGCAATCCTCTGGGTTGGGACATGCCGAAATTCACGATGTAGATACTTTCAATCATGCGAGTCCCAATGGCACTGCACAAGCTGAAGTCACAAGCACCGGCAGTGGTAATCTATTAGATGCTACACCTGTGAAAAATGTAGAGGATTTGGAACATCAGAAAATTGGCATTGACTTGGAAGATTCAGGGGATGAAGCAACTCATAGTGCATTAATACAGGGTGGTGCGAGTAGAAGGAAGATGAGGATGATGGTACTCGAAGATGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1254

Amino Acids

141.41

Weight (kDa)

5.55

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 27 - 282 2.4e-59 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1599
Acc65I GGTACC 1 cut(s) 3280
AccB1I GGYRCC 1 cut(s) 3280
AccI GTMKAC 1 cut(s) 2743
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 1718
AciI CCGC 3 cut(s) 146, 371, 2613
AcoI YGGCCR 2 cut(s) 1846, 2392
AcuI CTGAAG 6 cut(s) 359, 1218, 2022, 2574, 2775, 3591
AdeI CACNNNGTG 1 cut(s) 2501
AfaI GTAC 8 cut(s) 95, 450, 459, 523, 1478, 1960, 3282, 3741
AfeI AGCGCT 1 cut(s) 1195
AfiI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
AflII CTTAAG 4 cut(s) 1424, 2977, 3110, 3350
AhlI ACTAGT 2 cut(s) 1306, 2668
AjnI CCWGG 4 cut(s) 48, 1071, 1843, 2151
AjuI GAANNNNNNNTTGG 2 cut(s) 2238, 2270
Alw21I GWGCWC 3 cut(s) 222, 2160, 2526
Alw26I GTCTC 3 cut(s) 773, 799, 1739
Ama87I CYCGRG 1 cut(s) 1894
Aor13HI TCCGGA 1 cut(s) 1718
Aor51HI AGCGCT 1 cut(s) 1195
AoxI GGCC 5 cut(s) 34, 1846, 2392, 2617, 2945
ApeKI GCWGC 6 cut(s) 237, 505, 1376, 1991, 2579, 3115
AseI ATTAAT 3 cut(s) 2364, 3239, 3698
Asp700I GAANNNNTTC 3 cut(s) 1008, 1401, 1623
Asp718I GGTACC 1 cut(s) 3280
AspLEI GCGC 3 cut(s) 138, 1196, 2850
AspS9I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 9 cut(s) 34, 632, 1268, 1775, 1943, 1955, 1976, 1988, 2513
AvaI CYCGRG 1 cut(s) 1894
AvaII GGWCC 4 cut(s) 941, 1268, 2648, 3178
BalI TGGCCA 2 cut(s) 1848, 2394
BamHI GGATCC 1 cut(s) 3365
BanI GGYRCC 1 cut(s) 3280
BanII GRGCYC 1 cut(s) 2160
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 6 cut(s) 336, 895, 1446, 1886, 2154, 2345
Bbv12I GWGCWC 3 cut(s) 222, 2160, 2526
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 6 cut(s) 249, 517, 1388, 1978, 2566, 3127
BceAI ACGGC 2 cut(s) 49, 1625
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 1049, 1083
BciT130I CCWGG 4 cut(s) 50, 1073, 1845, 2153
BciVI GTATCC 1 cut(s) 202
BclI TGATCA 3 cut(s) 622, 1492, 3016
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 3 cut(s) 773, 799, 1739
BcuI ACTAGT 2 cut(s) 1306, 2668
BfmI CTRYAG 3 cut(s) 750, 917, 1374
BfoI RGCGCY 1 cut(s) 1197
BfrI CTTAAG 4 cut(s) 1424, 2977, 3110, 3350
BfuI GTATCC 1 cut(s) 202
BglII AGATCT 2 cut(s) 1579, 3005
BisI GCNGC 6 cut(s) 238, 506, 1377, 1992, 2580, 3116
BlpI GCTNAGC 3 cut(s) 2214, 2874, 3338
BlsI GCNGC 6 cut(s) 239, 507, 1378, 1993, 2581, 3117
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 5 cut(s) 50, 155, 1073, 1845, 2153
Bme18I GGWCC 4 cut(s) 941, 1268, 2648, 3178
BmeT110I CYCGRG 1 cut(s) 1894
BmgT120I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
BmiI GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
BmrFI CCNGG 5 cut(s) 50, 155, 1073, 1845, 2153
BmrI ACTGGG 1 cut(s) 2428
BmsI GCATC 8 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591, 3595
BmuI ACTGGG 1 cut(s) 2428
BpiI GAAGAC 6 cut(s) 336, 895, 1446, 1886, 2154, 2345
BplI GAGNNNNNCTC 2 cut(s) 3479, 3511
BpmI CTGGAG 2 cut(s) 2174, 3171
Bpu10I CCTNAGC 4 cut(s) 122, 732, 1190, 3360
Bpu1102I GCTNAGC 3 cut(s) 2214, 2874, 3338
BpuEI CTTGAG 3 cut(s) 151, 755, 3078
BpuMI CCSGG 1 cut(s) 155
Bsa29I ATCGAT 1 cut(s) 3407
BsaJI CCNNGG 8 cut(s) 37, 48, 195, 858, 1446, 1500, 1843, 2971
BsaWI WCCGGW 2 cut(s) 69, 1718
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
Bse118I RCCGGY 1 cut(s) 3584
Bse1I ACTGG 7 cut(s) 284, 528, 1027, 1228, 2423, 2620, 2644
Bse3DI GCAATG 4 cut(s) 500, 1272, 2242, 3069
BseAI TCCGGA 1 cut(s) 1718
BseBI CCWGG 4 cut(s) 50, 1073, 1845, 2153
BseCI ATCGAT 1 cut(s) 3407
BseDI CCNNGG 8 cut(s) 37, 48, 195, 858, 1446, 1500, 1843, 2971
BseLI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
BseMI GCAATG 4 cut(s) 500, 1272, 2242, 3069
BseMII CTCAG 7 cut(s) 113, 1151, 1181, 1679, 2205, 2865, 3149
BseNI ACTGG 7 cut(s) 284, 528, 1027, 1228, 2423, 2620, 2644
BseRI GAGGAG 5 cut(s) 1517, 1693, 2177, 3081, 3332
BseXI GCAGC 6 cut(s) 249, 517, 1388, 1978, 2566, 3127
BsgI GTGCAG 1 cut(s) 3546
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BshNI GGYRCC 1 cut(s) 3280
BshVI ATCGAT 1 cut(s) 3407
BsiHKAI GWGCWC 3 cut(s) 222, 2160, 2526
BsiHKCI CYCGRG 1 cut(s) 1894
BsiSI CCGG 4 cut(s) 70, 154, 1719, 3585
BslFI GGGAC 3 cut(s) 853, 3519, 3534
BslI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
BsmAI GTCTC 3 cut(s) 773, 799, 1739
BsmFI GGGAC 3 cut(s) 853, 3519, 3534
BsmI GAATGC 2 cut(s) 1168, 1331
BsnI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BsoBI CYCGRG 1 cut(s) 1894
Bsp1286I GDGCHC 3 cut(s) 222, 2160, 2526
Bsp13I TCCGGA 1 cut(s) 1718
Bsp1407I TGTACA 1 cut(s) 1476
Bsp1720I GCTNAGC 3 cut(s) 2214, 2874, 3338
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 3 cut(s) 146, 371, 2613
BspANI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BspCNI CTCAG 7 cut(s) 114, 1150, 1182, 1680, 2206, 2866, 3148
BspDI ATCGAT 1 cut(s) 3407
BspEI TCCGGA 1 cut(s) 1718
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 3 cut(s) 643, 1012, 2929
BspLI GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
BspMAI CTGCAG 1 cut(s) 1378
BspT107I GGYRCC 1 cut(s) 3280
BspTI CTTAAG 4 cut(s) 1424, 2977, 3110, 3350
BsrDI GCAATG 4 cut(s) 500, 1272, 2242, 3069
BsrFI RCCGGY 1 cut(s) 3584
BsrGI TGTACA 1 cut(s) 1476
BsrI ACTGG 7 cut(s) 284, 528, 1027, 1228, 2423, 2620, 2644
BssAI RCCGGY 1 cut(s) 3584
BssECI CCNNGG 8 cut(s) 37, 48, 195, 858, 1446, 1500, 1843, 2971
BssT1I CCWWGG 3 cut(s) 195, 858, 1446
Bst2UI CCWGG 4 cut(s) 50, 1073, 1845, 2153
Bst4CI ACNGT 3 cut(s) 1735, 1911, 2917
Bst6I CTCTTC 8 cut(s) 783, 1100, 1670, 1975, 2336, 3137, 3295, 3309
BstAFI CTTAAG 4 cut(s) 1424, 2977, 3110, 3350
BstAUI TGTACA 1 cut(s) 1476
BstC8I GCNNGC 2 cut(s) 1409, 1465
BstDSI CCRYGG 2 cut(s) 858, 2971
BstENI CCTNNNNNAGG 1 cut(s) 1857
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 1197
BstHHI GCGC 3 cut(s) 138, 1196, 2850
BstMAI GTCTC 3 cut(s) 773, 799, 1739
BstMWI GCNNNNNNNGC 4 cut(s) 552, 1845, 2812, 2993
BstNI CCWGG 4 cut(s) 50, 1073, 1845, 2153
BstNSI RCATGY 4 cut(s) 1411, 2131, 3163, 3512
BstSCI CCNGG 5 cut(s) 48, 153, 1071, 1843, 2151
BstSFI CTRYAG 3 cut(s) 750, 917, 1374
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 249, 517, 1388, 1978, 2566, 3127
BstV2I GAAGAC 6 cut(s) 336, 895, 1446, 1886, 2154, 2345
BstX2I RGATCY 4 cut(s) 1579, 2704, 3005, 3365
BstXI CCANNNNNNTGG 1 cut(s) 3064
BstYI RGATCY 4 cut(s) 1579, 2704, 3005, 3365
Bsu15I ATCGAT 1 cut(s) 3407
BsuI GTATCC 1 cut(s) 202
BsuRI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BsuTUI ATCGAT 1 cut(s) 3407
BtgI CCRYGG 2 cut(s) 858, 2971
BtgZI GCGATG 2 cut(s) 153, 2589
BtsI GCAGTG 4 cut(s) 220, 261, 3558, 3595
BtsIMutI CAGTG 7 cut(s) 220, 261, 291, 1221, 2149, 3558, 3595
Cac8I GCNNGC 2 cut(s) 1409, 1465
CciI TCATGA 3 cut(s) 643, 1012, 2929
CfoI GCGC 3 cut(s) 138, 1196, 2850
Cfr10I RCCGGY 1 cut(s) 3584
Cfr13I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
ClaI ATCGAT 1 cut(s) 3407
Csp6I GTAC 8 cut(s) 94, 449, 458, 522, 1477, 1959, 3281, 3740
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviQI GTAC 8 cut(s) 94, 449, 458, 522, 1477, 1959, 3281, 3740
DraI TTTAAA 2 cut(s) 249, 3025
DraIII CACNNNGTG 1 cut(s) 2501
EaeI YGGCCR 2 cut(s) 1846, 2392
Eam1104I CTCTTC 8 cut(s) 783, 1100, 1670, 1975, 2336, 3137, 3295, 3309
EarI CTCTTC 8 cut(s) 783, 1100, 1670, 1975, 2336, 3137, 3295, 3309
Ecl136II GAGCTC 1 cut(s) 2158
Eco130I CCWWGG 3 cut(s) 195, 858, 1446
Eco24I GRGCYC 1 cut(s) 2160
Eco32I GATATC 1 cut(s) 2206
Eco47I GGWCC 4 cut(s) 941, 1268, 2648, 3178
Eco47III AGCGCT 1 cut(s) 1195
Eco53kI GAGCTC 1 cut(s) 2158
Eco57I CTGAAG 6 cut(s) 359, 1218, 2022, 2574, 2775, 3591
Eco88I CYCGRG 1 cut(s) 1894
EcoICRI GAGCTC 1 cut(s) 2158
EcoNI CCTNNNNNAGG 1 cut(s) 1857
EcoO109I RGGNCCY 2 cut(s) 2648, 3178
EcoRI GAATTC 1 cut(s) 2221
EcoRII CCWGG 4 cut(s) 48, 1071, 1843, 2151
EcoRV GATATC 1 cut(s) 2206
EcoT14I CCWWGG 3 cut(s) 195, 858, 1446
EcoT22I ATGCAT 3 cut(s) 718, 1333, 2129
EcoT38I GRGCYC 1 cut(s) 2160
ErhI CCWWGG 3 cut(s) 195, 858, 1446
FaqI GGGAC 3 cut(s) 853, 3519, 3534
FauI CCCGC 1 cut(s) 2606
FbaI TGATCA 3 cut(s) 622, 1492, 3016
FblI GTMKAC 1 cut(s) 2743
Fnu4HI GCNGC 6 cut(s) 238, 506, 1377, 1992, 2580, 3116
FriOI GRGCYC 1 cut(s) 2160
Fsp4HI GCNGC 6 cut(s) 238, 506, 1377, 1992, 2580, 3116
GlaI GCGC 3 cut(s) 137, 1195, 2849
GluI GCNGC 6 cut(s) 238, 506, 1377, 1992, 2580, 3116
GsuI CTGGAG 2 cut(s) 2174, 3171
HaeII RGCGCY 1 cut(s) 1197
HaeIII GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
HapII CCGG 4 cut(s) 70, 154, 1719, 3585
HhaI GCGC 3 cut(s) 138, 1196, 2850
Hin6I GCGC 3 cut(s) 136, 1194, 2848
HinP1I GCGC 3 cut(s) 136, 1194, 2848
HincII GTYRAC 2 cut(s) 1024, 3484
HindII GTYRAC 2 cut(s) 1024, 3484
HindIII AAGCTT 2 cut(s) 1712, 2804
HpaI GTTAAC 2 cut(s) 1024, 3484
HpaII CCGG 4 cut(s) 70, 154, 1719, 3585
HphI GGTGA 9 cut(s) 34, 632, 1268, 1775, 1943, 1955, 1976, 1988, 2513
Hpy166II GTNNAC 8 cut(s) 398, 426, 1024, 1268, 1766, 2744, 3107, 3484
Hpy8I GTNNAC 8 cut(s) 398, 426, 1024, 1268, 1766, 2744, 3107, 3484
Hpy99I CGWCG 4 cut(s) 59, 62, 65, 939
HpyCH4III ACNGT 3 cut(s) 1735, 1911, 2917
HpyCH4IV ACGT 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 552, 1845, 2812, 2993
HpySE526I ACGT 1 cut(s) 353
HspAI GCGC 3 cut(s) 136, 1194, 2848
Kpn2I TCCGGA 1 cut(s) 1718
KpnI GGTACC 1 cut(s) 3284
Ksp22I TGATCA 3 cut(s) 622, 1492, 3016
KspAI GTTAAC 2 cut(s) 1024, 3484
LmnI GCTCC 6 cut(s) 199, 334, 1450, 2155, 3094, 3152
Lsp1109I GCAGC 6 cut(s) 249, 517, 1388, 1978, 2566, 3127
LweI GCATC 8 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591, 3595
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 8 cut(s) 77, 359, 1043, 1144, 1786, 1964, 2093, 3574
MfeI CAATTG 3 cut(s) 1209, 2396, 2734
MflI RGATCY 4 cut(s) 1579, 2704, 3005, 3365
MhlI GDGCHC 3 cut(s) 222, 2160, 2526
MlsI TGGCCA 2 cut(s) 1848, 2394
MluNI TGGCCA 2 cut(s) 1848, 2394
MlyI GAGTC 8 cut(s) 1301, 1436, 1961, 2335, 2814, 2976, 3329, 3556
MmeI TCCRAC 2 cut(s) 1327, 2823
Mox20I TGGCCA 2 cut(s) 1848, 2394
Mph1103I ATGCAT 3 cut(s) 718, 1333, 2129
MroI TCCGGA 1 cut(s) 1718
MroXI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
MscI TGGCCA 2 cut(s) 1848, 2394
MslI CAYNNNNRTG 2 cut(s) 618, 3164
Msp20I TGGCCA 2 cut(s) 1848, 2394
MspA1I CMGCKG 2 cut(s) 454, 1379
MspCI CTTAAG 4 cut(s) 1424, 2977, 3110, 3350
MspI CCGG 4 cut(s) 70, 154, 1719, 3585
MspR9I CCNGG 5 cut(s) 50, 155, 1073, 1845, 2153
MunI CAATTG 3 cut(s) 1209, 2396, 2734
Mva1269I GAATGC 2 cut(s) 1168, 1331
MvaI CCWGG 4 cut(s) 50, 1073, 1845, 2153
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 552, 1845, 2812, 2993
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 858
NlaIV GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
NmeAIII GCCGAG 1 cut(s) 2923
NmuCI GTSAC 3 cut(s) 359, 1964, 3574
NsiI ATGCAT 3 cut(s) 718, 1333, 2129
NspI RCATGY 4 cut(s) 1411, 2131, 3163, 3512
PaeI GCATGC 1 cut(s) 1411
PaeR7I CTCGAG 1 cut(s) 1894
PagI TCATGA 3 cut(s) 643, 1012, 2929
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 1168, 1331
PdmI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
PfeI GAWTC 8 cut(s) 42, 755, 1402, 2861, 2933, 3188, 3409, 3668
PkrI GCNGC 6 cut(s) 239, 507, 1378, 1993, 2581, 3117
PleI GAGTC 8 cut(s) 1300, 1436, 1961, 2335, 2814, 2975, 3328, 3555
PpsI GAGTC 8 cut(s) 1300, 1436, 1961, 2335, 2814, 2975, 3328, 3555
PpuMI RGGWCCY 2 cut(s) 2648, 3178
PshBI ATTAAT 3 cut(s) 2364, 3239, 3698
PsiI TTATAA 1 cut(s) 1599
Psp124BI GAGCTC 1 cut(s) 2160
Psp5II RGGWCCY 2 cut(s) 2648, 3178
Psp6I CCWGG 4 cut(s) 48, 1071, 1843, 2151
PspGI CCWGG 4 cut(s) 48, 1071, 1843, 2151
PspN4I GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
PspPI GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
PspPPI RGGWCCY 2 cut(s) 2648, 3178
PstI CTGCAG 1 cut(s) 1378
PsuI RGATCY 4 cut(s) 1579, 2704, 3005, 3365
PvuII CAGCTG 2 cut(s) 454, 1379
RsaI GTAC 8 cut(s) 95, 450, 459, 523, 1478, 1960, 3282, 3741
RsaNI GTAC 8 cut(s) 94, 449, 458, 522, 1477, 1959, 3281, 3740
RseI CAYNNNNRTG 2 cut(s) 618, 3164
SacI GAGCTC 1 cut(s) 2160
SatI GCNGC 6 cut(s) 238, 506, 1377, 1992, 2580, 3116
Sau96I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 8 cut(s) 1301, 1436, 1961, 2335, 2814, 2976, 3329, 3556
ScrFI CCNGG 5 cut(s) 50, 155, 1073, 1845, 2153
SduI GDGCHC 3 cut(s) 222, 2160, 2526
SfaNI GCATC 8 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591, 3595
SfcI CTRYAG 3 cut(s) 750, 917, 1374
Sfr274I CTCGAG 1 cut(s) 1894
SinI GGWCC 4 cut(s) 941, 1268, 2648, 3178
SlaI CTCGAG 1 cut(s) 1894
SmiMI CAYNNNNRTG 2 cut(s) 618, 3164
SmlI CTYRAG 8 cut(s) 130, 770, 1424, 1894, 2977, 3093, 3110, 3350
SmoI CTYRAG 8 cut(s) 130, 770, 1424, 1894, 2977, 3093, 3110, 3350
SpeI ACTAGT 2 cut(s) 1306, 2668
SphI GCATGC 1 cut(s) 1411
SsiI CCGC 3 cut(s) 146, 371, 2613
SspI AATATT 2 cut(s) 187, 2375
SstI GAGCTC 1 cut(s) 2160
StyD4I CCNGG 5 cut(s) 48, 153, 1071, 1843, 2151
StyI CCWWGG 3 cut(s) 195, 858, 1446
TaaI ACNGT 3 cut(s) 1735, 1911, 2917
TaiI ACGT 1 cut(s) 356
TaqII GACCGA 1 cut(s) 1514
TatI WGTACW 3 cut(s) 93, 1476, 1958
TfiI GAWTC 8 cut(s) 42, 755, 1402, 2861, 2933, 3188, 3409, 3668
TscAI CASTG 7 cut(s) 227, 261, 291, 1228, 2149, 3565, 3595
TseFI GTSAC 3 cut(s) 359, 1964, 3574
TseI GCWGC 6 cut(s) 237, 505, 1376, 1991, 2579, 3115
Tsp45I GTSAC 3 cut(s) 359, 1964, 3574
TspGWI ACGGA 2 cut(s) 78, 2960
TspRI CASTG 7 cut(s) 227, 261, 291, 1228, 2149, 3565, 3595
Vha464I CTTAAG 4 cut(s) 1424, 2977, 3110, 3350
VpaK11BI GGWCC 4 cut(s) 941, 1268, 2648, 3178
VspI ATTAAT 3 cut(s) 2364, 3239, 3698
XagI CCTNNNNNAGG 1 cut(s) 1857
XceI RCATGY 4 cut(s) 1411, 2131, 3163, 3512
XhoI CTCGAG 1 cut(s) 1894
XmiI GTMKAC 1 cut(s) 2743
XmnI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
ZrmI AGTACT 1 cut(s) 95
Zsp2I ATGCAT 3 cut(s) 718, 1333, 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.