FvH4_7g30740

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007218
Physical Location & Seq
Reverse (-)
22320490 .. 22329404
8915 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30740.t2

Sequence Viewer

Length: 3774 bp
ATGGATTTGCAAGGCTTATCGGTCATCTGCAACGGCCTCGGAATCGCCCTAGACGACGACGACGGTAACCGGATCGGCTACCGGAAGCACAAATACTGCTTAGATAATTTGAAAGATTTGCTGAGGTTCTTGCGGCGCGACGATCCGCAGAATCGGGATGTTTTCAAGGAAGTTTGCAAGTGGAATATTTTATCTAAGGATTTGATACCTATTATTGAGCACTGCCAGGAGGATCGTAGCTTGGTTTTGAATGCAGTGAAGGTTCTGGTGTTTCTTACTATGCCGGTTGAGCCTACATCAAATGACGTTTTACAGCAAACAGAATATCTTTGGGAGTTGAAGTCTTCAATTACGTCTAGTGACATTGTCGCGGTTATAGTATCACTTTTGGAAAGTCCACTAGAAAATCTGGAAAGTGATGTGTTCACAGAGGACGACTGGAAATTGGTGCAGCTGGTACTGACTTTGTTCCGAAACATTTTAGCCATTCAAGATATTTCATTGCACCAGAAGTCTGGGGGAACTGCAAGTCAATTTCTGTCACGCAGAGATAGCTTTCTGGAACTTTTGTTTCGTGAAAATGTGATGGACTTGGTTTTGGTCATAACACAGCATATTGGTGATCATCGTAGTTATCTCTCTCATGACAACTTGCTTCTATTGGAAATTTTCCATTATACATTTATGGGGCAAGAGCCAGAGTTGATTGCAAATGCATATTCAAAAGGAAAAGGTGCTAAGGTGGAAGAAGATACCATAGATTCTGTAAATAGTCTCAAGTCTATTATGGAGGAAGAGAAAGACAAAAAAAGACTAAAAGTTAGTCGTCATCCCAATTTTGGTGGGACATTTATTCGGCTTACCATGGATGGTTCTAAGGAAGTTGTCAAGGGAAGACCTTCTGCGTCTTGTAATACAATGCAGAAACCACAAAATCATCGTGGTCCAATAAAAACAATTGCTTGGGATCATGGTATATTACCTTCAACTGAGGATAAGATTTTGGAATTGCTTCATGACTTTGTGAACCAGTTTTTATCAGGGGGTTACAATGTTTTGATGCAGTCAATTCGTCAGGCCATTGAAAAGGAACACCACTCGATCCAGAAAAGTGATGTCATTATCTTCTTTCAGGTTGCTCAGTTTGTTACTTCTTTCCAATATCACAGGCATTCAAGTTCTAAGCTGAGCCTGAGAGCTGAAGCAGATACAATTGAAGTTCTGTCCAGTAAAGAGGCTGATAGAACACTCTTCAGAGGTGATATATGTGGGCCCATTGCAACCTCAATGAACGAGTCCATGTTCCAACTAGTCATTTCAAAGTGGCGTTATGCATTTGATGGGTTAAAGGAAACACATGACTACAAGTTTCTGTCTGCAGCTGGTTCTCTTATGAAAGAAATGATCCGCATGCTAGATCTGGTGCTTAAGTTATTGCCTGAGGACTCCAAGGAGCCTCAAACAGCTCGCATTCTTCTGTATAAGTTATTTTATGACCAGACCGAAGAAGGGATGACCCATTTTCTCTTAAGTTTGCTGAAATCGTTCGACACTCACAAACAACCAAGAAGTGATCTTGCAGATCTGGTAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCATCAGGAACATTGAGGGTTTCTAAAAAGTCAAGGAAAGGTAGGAAGAGGAGAAAAGTGAGTGAGAAGGAAACAGAAAATAAGCTCTCAGGAGAGCATGATATGGTTCAGAAAGAGGCTGACATCTCCAAGGGTGAACAATCAACAGATATGCATGTAACTGAGAAAAGATGCCTAGATACAAGTTCTGATGGGAAACAAGACATCAGCATCCCTGGCCAACCTGATGAAGGCAAAAAAGCTTTACTGGAGACGGAAAACTTCCCGGGCAGTCAGGCACAGTTAGATCACAAAAATTCTGGTGATGTGAATGATGATCTTGGTTATAGTACAGGTGACTCTTCTGGTGATGAGCAAGTACCTGCAACTGCTGAAGTTGATTTTAAGGTGTCAACTTTGGTTTCTGCTTTTTCAAACCACAGCATCATCCAGAAATTGTGTTGGTTGCTTAAGTTCTATAAGAGTAACTTGACCAGAACAAATCACTACATAATATGCATGTTGCGAAGGATCAGCGAAGACCTGGAGCTCTCTCCAATGCTGTATCAGTTATCACTCCTGACTATATTCTATGATATTCTAGCTGAGCAAAAGTCATCTGCTTGCAACGAATATGAAAACATTGTTGGTTTCTTGAAAAGTTTGGTCAGGAAAATGCTAAAAAAAATGAAACAGCAACCCTTACTTTTTGTGGAAATTCTCTTCTGGAAGACTCGCAAAGAGTGTCATTACATTAATGCTGAATATCTATTGCATGAGCTTGGCCAAATGAGGAACGAAAGTAGAAACTGGGGAGATACTTTAGAGGATGGAGAAACGGGTCGATCAACAGACAAGGGGTGGACAGGTAGAAGCCTTGCAGATGCACTTGGTGAAGATGAAGCTGATGTTGTGCTTGATTTTGGACATGACAACAATGAAGAAAATCTTGGAAACGTGGAAGGAGATGCTGCGTCTACTTCAGATAATGAGATTGATGGGAAAAAAGATCATGACAATGGGGTCCAGCCTATGAAAACTGAAACTGGAAGGGGTCCCCTTAAAAAGAAAAGACTTGTTATTGGAGAGGAACTGGAGATGAAAATTAAGGATCTCTATGAGAAATTCAAGGATAACCACAATTGTAGTCAGCTCATTGCTGAAGTACTTGATCCTGATGGGAAAATTTTGTCAACTCAAATTTCCAACAAGCTTAAACATCTTGGACTCAAAGTTGCACGAAGGAAAAGATTACGCCATGCGCAGGAATCTGTTTCTGCCGAGCCTAGTCAACCTGATGGAGATAATGAATTAAAGGAAACAGCTATTAGTCATCATGAATCAAAAAGTCGGCCTTTGCACACAAGAAAAAGAGTCCGTGGCTTAAGCGAAGGACAGGAAGCTCAAATCAGATCTCTTTATGATCAGTTTAAAGACCATAAAACTTGCAGCCACATGATTGCTAATGCAATGGATGGTGATGATAAACTGACAGCTCCTCAAGTTTCTCATAAACTGAAGCAGCTTGGTTTATATATTCCTCAGAAGAAGAGGTCTGGAGCTAACAGGCGGATGAGGGATCAGGAACTAAATGATTCCAACACAAACGACGCTAATGCCTCTGATGATGAAACATTGTTATCGTTAATGAATAGGGGCAAAAAGGATAACATCAGATTCAATGAGGTACCAGAACAAACAGCTAGAAGAGAAGTGTCAGAAGATGAGTCTGATGATGAAATGTTAAGCTCTGTTCTCAAGAAAGCTAGGGGATCCCTTTCTAATTTAAAGGATCAGAACCGTCCAGCCACCTCGATTCAAGGAACAGCAAGAGGAAATGCTTTAGAAAATGAAGTTGAGGAAGCGGTTGTATCTTCTGGGAGGGATGTTAACAAGCAATCCTCCGGATTGGGACTTGCTAAAAGTGATGATGGTGATACTTTCAATCAAGTGAGTTCCCATGGCACAGCACAAGCTGAAGTCACAAGCACCGGCAGTGGTGCACTATCAAATGTGGAAAATTTAGAGGATTTGCAACATGAGAAAATCGGCATTGACTTGGACGATTCAGGGGATGAAGCGACTGATAGTGCATTAACACAAGGTGGTGCAAGTAGAAGGAAGATGAGGATGATGGTACTGGAAGATGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1258

Amino Acids

141.95

Weight (kDa)

5.56

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 27 - 284 4.3e-60 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1602
AasI GACNNNNNNGTC 1 cut(s) 2633
Acc16I TGCGCA 1 cut(s) 2874
Acc36I ACCTGC 1 cut(s) 2002
Acc65I GGTACC 1 cut(s) 3298
AccB1I GGYRCC 1 cut(s) 3298
AccI GTMKAC 1 cut(s) 2588
AccII CGCG 2 cut(s) 138, 371
AccIII TCCGGA 1 cut(s) 3515
AciI CCGC 6 cut(s) 133, 146, 371, 1408, 3181, 3476
AcoI YGGCCR 2 cut(s) 1849, 2395
AcsI RAATTY 7 cut(s) 666, 1927, 2328, 2735, 2796, 2811, 3631
AcuI CTGAAG 7 cut(s) 1221, 1237, 2025, 2577, 2793, 3149, 3609
AdeI CACNNNGTG 2 cut(s) 2504, 3716
AfaI GTAC 6 cut(s) 459, 1963, 1992, 2778, 3300, 3750
AfiI CCNNNNNNNGG 4 cut(s) 839, 1509, 1862, 2875
AflII CTTAAG 4 cut(s) 1427, 1528, 2081, 2995
AhlI ACTAGT 1 cut(s) 1309
AjnI CCWGG 3 cut(s) 225, 1846, 2154
AjuI GAANNNNNNNTTGG 4 cut(s) 2241, 2273, 2544, 2576
Alw21I GWGCWC 3 cut(s) 222, 2163, 3616
Alw26I GTCTC 2 cut(s) 779, 1877
Alw44I GTGCAC 1 cut(s) 3612
Ama87I CYCGRG 1 cut(s) 1897
Aor13HI TCCGGA 1 cut(s) 3515
AoxI GGCC 6 cut(s) 34, 1077, 1271, 1849, 2395, 2963
ApaI GGGCCC 1 cut(s) 1275
ApaLI GTGCAC 1 cut(s) 3612
ApeKI GCWGC 5 cut(s) 451, 1379, 2582, 3060, 3133
ApoI RAATTY 7 cut(s) 666, 1927, 2328, 2735, 2796, 2811, 3631
AseI ATTAAT 1 cut(s) 2367
Asp700I GAANNNNTTC 4 cut(s) 898, 1011, 1544, 1626
Asp718I GGTACC 1 cut(s) 3298
AspLEI GCGC 2 cut(s) 138, 2875
AspS9I GGNCC 5 cut(s) 944, 1271, 1272, 2635, 2666
AsuC2I CCSGG 2 cut(s) 1898, 1899
AsuHPI GGTGA 9 cut(s) 632, 1271, 1778, 1946, 1979, 1991, 2516, 3101, 3557
AvaI CYCGRG 1 cut(s) 1897
AvaII GGWCC 3 cut(s) 944, 2635, 2666
AxyI CCTNAGG 1 cut(s) 1440
BaeGI GKGCMC 2 cut(s) 1275, 3616
BalI TGGCCA 2 cut(s) 1851, 2397
BamHI GGATCC 1 cut(s) 3383
BanI GGYRCC 1 cut(s) 3298
BanII GRGCYC 2 cut(s) 1275, 2163
BarI GAAGNNNNNNTAC 4 cut(s) 77, 109, 967, 999
BbsI GAAGAC 4 cut(s) 336, 901, 2157, 2348
Bbv12I GWGCWC 3 cut(s) 222, 2163, 3616
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 5 cut(s) 463, 1391, 2569, 3072, 3145
BceAI ACGGC 2 cut(s) 49, 1628
BcgI CGANNNNNNTGC 6 cut(s) 19, 53, 1052, 1086, 3209, 3243
BciT130I CCWGG 3 cut(s) 227, 1848, 2156
BclI TGATCA 2 cut(s) 622, 3034
BcnI CCSGG 2 cut(s) 1898, 1899
BcoDI GTCTC 2 cut(s) 779, 1877
BcuI ACTAGT 1 cut(s) 1309
BfmI CTRYAG 1 cut(s) 1377
BfrI CTTAAG 4 cut(s) 1427, 1528, 2081, 2995
BfuAI ACCTGC 1 cut(s) 2002
BglII AGATCT 3 cut(s) 1417, 1582, 3023
BisI GCNGC 6 cut(s) 134, 452, 1380, 2583, 3061, 3134
BlpI GCTNAGC 2 cut(s) 1187, 2217
BlsI GCNGC 6 cut(s) 135, 453, 1381, 2584, 3062, 3135
BmcAI AGTACT 1 cut(s) 2778
Bme1390I CCNGG 5 cut(s) 227, 1848, 1898, 1899, 2156
Bme18I GGWCC 3 cut(s) 944, 2635, 2666
BmeT110I CYCGRG 1 cut(s) 1897
BmgT120I GGNCC 5 cut(s) 944, 1271, 1272, 2635, 2666
BmiI GGNNCC 7 cut(s) 1273, 1455, 2636, 2667, 2668, 3300, 3385
BmrFI CCNGG 5 cut(s) 227, 1848, 1898, 1899, 2156
BmrI ACTGGG 1 cut(s) 2431
BmsI GCATC 7 cut(s) 1050, 1643, 1793, 1851, 2064, 2485, 2569
BmuI ACTGGG 1 cut(s) 2431
BpiI GAAGAC 4 cut(s) 336, 901, 2157, 2348
BpmI CTGGAG 4 cut(s) 1901, 2177, 2726, 3189
Bpu10I CCTNAGC 2 cut(s) 122, 738
Bpu1102I GCTNAGC 2 cut(s) 1187, 2217
BpuEI CTTGAG 3 cut(s) 761, 3096, 3353
BpuMI CCSGG 2 cut(s) 1898, 1899
BsaJI CCNNGG 8 cut(s) 37, 864, 1449, 1761, 1846, 1897, 2989, 3571
BsaWI WCCGGW 3 cut(s) 69, 81, 3515
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 4 cut(s) 839, 1509, 1862, 2875
Bse118I RCCGGY 2 cut(s) 283, 3602
Bse1I ACTGG 8 cut(s) 443, 1030, 1227, 1884, 2426, 2662, 2709, 3756
Bse21I CCTNAGG 1 cut(s) 1440
Bse3DI GCAATG 4 cut(s) 500, 1275, 2766, 3087
BseAI TCCGGA 1 cut(s) 3515
BseBI CCWGG 3 cut(s) 227, 1848, 2156
BseDI CCNNGG 8 cut(s) 37, 864, 1449, 1761, 1846, 1897, 2989, 3571
BseLI CCNNNNNNNGG 4 cut(s) 839, 1509, 1862, 2875
BseMI GCAATG 4 cut(s) 500, 1275, 2766, 3087
BseNI ACTGG 8 cut(s) 443, 1030, 1227, 1884, 2426, 2662, 2709, 3756
BseRI GAGGAG 2 cut(s) 1696, 3099
BseSI GKGCMC 2 cut(s) 1275, 3616
BseXI GCAGC 5 cut(s) 463, 1391, 2569, 3072, 3145
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 2 cut(s) 138, 371
BshFI GGCC 6 cut(s) 36, 1079, 1273, 1851, 2397, 2965
BshNI GGYRCC 1 cut(s) 3298
BsiHKAI GWGCWC 3 cut(s) 222, 2163, 3616
BsiHKCI CYCGRG 1 cut(s) 1897
BsiSI CCGG 6 cut(s) 70, 82, 284, 1898, 3516, 3603
BslFI GGGAC 3 cut(s) 859, 2652, 3537
BslI CCNNNNNNNGG 4 cut(s) 839, 1509, 1862, 2875
BsmAI GTCTC 2 cut(s) 779, 1877
BsmBI CGTCTC 1 cut(s) 1877
BsmFI GGGAC 3 cut(s) 859, 2652, 3537
BsmI GAATGC 3 cut(s) 256, 1171, 1470
BsnI GGCC 6 cut(s) 36, 1079, 1273, 1851, 2397, 2965
BsoBI CYCGRG 1 cut(s) 1897
Bsp120I GGGCCC 1 cut(s) 1271
Bsp1286I GDGCHC 4 cut(s) 222, 1275, 2163, 3616
Bsp13I TCCGGA 1 cut(s) 3515
Bsp1720I GCTNAGC 2 cut(s) 1187, 2217
Bsp19I CCATGG 2 cut(s) 864, 3571
BspACI CCGC 6 cut(s) 133, 146, 371, 1408, 3181, 3476
BspANI GGCC 6 cut(s) 36, 1079, 1273, 1851, 2397, 2965
BspEI TCCGGA 1 cut(s) 3515
BspFNI CGCG 2 cut(s) 138, 371
BspHI TCATGA 4 cut(s) 643, 1015, 2623, 2947
BspLI GGNNCC 7 cut(s) 1273, 1455, 2636, 2667, 2668, 3300, 3385
BspMAI CTGCAG 1 cut(s) 1381
BspMI ACCTGC 1 cut(s) 2002
BspT107I GGYRCC 1 cut(s) 3298
BspTI CTTAAG 4 cut(s) 1427, 1528, 2081, 2995
BsrDI GCAATG 4 cut(s) 500, 1275, 2766, 3087
BsrFI RCCGGY 2 cut(s) 283, 3602
BsrI ACTGG 8 cut(s) 443, 1030, 1227, 1884, 2426, 2662, 2709, 3756
BssAI RCCGGY 2 cut(s) 283, 3602
BssECI CCNNGG 8 cut(s) 37, 864, 1449, 1761, 1846, 1897, 2989, 3571
BssT1I CCWWGG 4 cut(s) 864, 1449, 1761, 3571
Bst2UI CCWGG 3 cut(s) 227, 1848, 2156
Bst4CI ACNGT 3 cut(s) 65, 1914, 3413
Bst6I CTCTTC 7 cut(s) 789, 1256, 1673, 1978, 2339, 3155, 3313
BstAFI CTTAAG 4 cut(s) 1427, 1528, 2081, 2995
BstC8I GCNNGC 3 cut(s) 1412, 1468, 2236
BstDSI CCRYGG 3 cut(s) 864, 2989, 3571
BstEII GGTNACC 1 cut(s) 65
BstENI CCTNNNNNAGG 1 cut(s) 1860
BstFNI CGCG 2 cut(s) 138, 371
BstHHI GCGC 2 cut(s) 138, 2875
BstMAI GTCTC 2 cut(s) 779, 1877
BstMWI GCNNNNNNNGC 3 cut(s) 289, 552, 1848
BstNI CCWGG 3 cut(s) 227, 1848, 2156
BstNSI RCATGY 3 cut(s) 1414, 1790, 2134
BstPI GGTNACC 1 cut(s) 65
BstSCI CCNGG 5 cut(s) 225, 1846, 1896, 1897, 2154
BstSFI CTRYAG 1 cut(s) 1377
BstSLI GKGCMC 2 cut(s) 1275, 3616
BstUI CGCG 2 cut(s) 138, 371
BstV1I GCAGC 5 cut(s) 463, 1391, 2569, 3072, 3145
BstV2I GAAGAC 4 cut(s) 336, 901, 2157, 2348
BstX2I RGATCY 5 cut(s) 1417, 1582, 2722, 3023, 3383
BstXI CCANNNNNNTGG 1 cut(s) 515
BstYI RGATCY 5 cut(s) 1417, 1582, 2722, 3023, 3383
Bsu36I CCTNAGG 1 cut(s) 1440
BsuRI GGCC 6 cut(s) 36, 1079, 1273, 1851, 2397, 2965
BtgI CCRYGG 3 cut(s) 864, 2989, 3571
BtsI GCAGTG 3 cut(s) 220, 261, 3613
BtsIMutI CAGTG 3 cut(s) 220, 261, 3613
BveI ACCTGC 1 cut(s) 2002
Cac8I GCNNGC 3 cut(s) 1412, 1468, 2236
CciI TCATGA 4 cut(s) 643, 1015, 2623, 2947
CfoI GCGC 2 cut(s) 138, 2875
Cfr10I RCCGGY 2 cut(s) 283, 3602
Cfr13I GGNCC 5 cut(s) 944, 1271, 1272, 2635, 2666
Cfr9I CCCGGG 1 cut(s) 1897
CseI GACGC 3 cut(s) 894, 2574, 3230
Csp6I GTAC 6 cut(s) 458, 1962, 1991, 2777, 3299, 3749
CspCI CAANNNNNGTGG 2 cut(s) 823, 858
CviQI GTAC 6 cut(s) 458, 1962, 1991, 2777, 3299, 3749
DraI TTTAAA 2 cut(s) 3043, 3399
DraIII CACNNNGTG 2 cut(s) 2504, 3716
DrdI GACNNNNNNGTC 1 cut(s) 2633
DseDI GACNNNNNNGTC 1 cut(s) 2633
EaeI YGGCCR 2 cut(s) 1849, 2395
Eam1104I CTCTTC 7 cut(s) 789, 1256, 1673, 1978, 2339, 3155, 3313
EarI CTCTTC 7 cut(s) 789, 1256, 1673, 1978, 2339, 3155, 3313
EciI GGCGGA 1 cut(s) 3196
Ecl136II GAGCTC 1 cut(s) 2161
Eco130I CCWWGG 4 cut(s) 864, 1449, 1761, 3571
Eco24I GRGCYC 2 cut(s) 1275, 2163
Eco47I GGWCC 3 cut(s) 944, 2635, 2666
Eco53kI GAGCTC 1 cut(s) 2161
Eco57I CTGAAG 7 cut(s) 1221, 1237, 2025, 2577, 2793, 3149, 3609
Eco81I CCTNAGG 1 cut(s) 1440
Eco88I CYCGRG 1 cut(s) 1897
Eco91I GGTNACC 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 2161
EcoNI CCTNNNNNAGG 1 cut(s) 1860
EcoO109I RGGNCCY 1 cut(s) 2666
EcoO65I GGTNACC 1 cut(s) 65
EcoRII CCWGG 3 cut(s) 225, 1846, 2154
EcoT14I CCWWGG 4 cut(s) 864, 1449, 1761, 3571
EcoT22I ATGCAT 4 cut(s) 718, 1336, 1788, 2132
EcoT38I GRGCYC 2 cut(s) 1275, 2163
ErhI CCWWGG 4 cut(s) 864, 1449, 1761, 3571
Esp3I CGTCTC 1 cut(s) 1877
FalI AAGNNNNNCTT 6 cut(s) 2084, 2116, 2544, 2576, 2950, 2982
FaqI GGGAC 3 cut(s) 859, 2652, 3537
FbaI TGATCA 2 cut(s) 622, 3034
FblI GTMKAC 1 cut(s) 2588
Fnu4HI GCNGC 6 cut(s) 134, 452, 1380, 2583, 3061, 3134
FriOI GRGCYC 2 cut(s) 1275, 2163
Fsp4HI GCNGC 6 cut(s) 134, 452, 1380, 2583, 3061, 3134
FspI TGCGCA 1 cut(s) 2874
GlaI GCGC 2 cut(s) 137, 2874
GluI GCNGC 6 cut(s) 134, 452, 1380, 2583, 3061, 3134
GsuI CTGGAG 4 cut(s) 1901, 2177, 2726, 3189
HaeIII GGCC 6 cut(s) 36, 1079, 1273, 1851, 2397, 2965
HapII CCGG 6 cut(s) 70, 82, 284, 1898, 3516, 3603
HgaI GACGC 3 cut(s) 894, 2574, 3230
HhaI GCGC 2 cut(s) 138, 2875
Hin6I GCGC 2 cut(s) 136, 2873
HinP1I GCGC 2 cut(s) 136, 2873
HincII GTYRAC 4 cut(s) 2025, 2805, 2903, 3502
HindII GTYRAC 4 cut(s) 2025, 2805, 2903, 3502
HindIII AAGCTT 2 cut(s) 1872, 2822
HpaI GTTAAC 1 cut(s) 3502
HpaII CCGG 6 cut(s) 70, 82, 284, 1898, 3516, 3603
HphI GGTGA 9 cut(s) 632, 1271, 1778, 1946, 1979, 1991, 2516, 3101, 3557
Hpy99I CGWCG 5 cut(s) 59, 62, 65, 143, 3224
HpyCH4III ACNGT 3 cut(s) 65, 1914, 3413
HpyCH4IV ACGT 3 cut(s) 306, 353, 2568
HpyF10VI GCNNNNNNNGC 3 cut(s) 289, 552, 1848
HpySE526I ACGT 3 cut(s) 306, 353, 2568
HspAI GCGC 2 cut(s) 136, 2873
KflI GGGWCCC 1 cut(s) 2666
Kpn2I TCCGGA 1 cut(s) 3515
KpnI GGTACC 1 cut(s) 3302
Ksp22I TGATCA 2 cut(s) 622, 3034
KspAI GTTAAC 1 cut(s) 3502
LmnI GCTCC 4 cut(s) 1453, 2158, 3112, 3170
Lsp1109I GCAGC 5 cut(s) 463, 1391, 2569, 3072, 3145
LweI GCATC 7 cut(s) 1050, 1643, 1793, 1851, 2064, 2485, 2569
MaeII ACGT 3 cut(s) 306, 353, 2568
MaeIII GTNAC 9 cut(s) 65, 359, 540, 1046, 1147, 1789, 1967, 2096, 3592
MfeI CAATTG 3 cut(s) 957, 1212, 2752
MflI RGATCY 5 cut(s) 1417, 1582, 2722, 3023, 3383
MhlI GDGCHC 4 cut(s) 222, 1275, 2163, 3616
MlsI TGGCCA 2 cut(s) 1851, 2397
MluNI TGGCCA 2 cut(s) 1851, 2397
MlyI GAGTC 7 cut(s) 1304, 1439, 1964, 2338, 2832, 2994, 3347
MmeI TCCRAC 3 cut(s) 1330, 2841, 3234
Mox20I TGGCCA 2 cut(s) 1851, 2397
Mph1103I ATGCAT 4 cut(s) 718, 1336, 1788, 2132
MroI TCCGGA 1 cut(s) 3515
MroXI GAANNNNTTC 4 cut(s) 898, 1011, 1544, 1626
MscI TGGCCA 2 cut(s) 1851, 2397
MslI CAYNNNNRTG 2 cut(s) 618, 2628
Msp20I TGGCCA 2 cut(s) 1851, 2397
MspA1I CMGCKG 2 cut(s) 454, 1382
MspCI CTTAAG 4 cut(s) 1427, 1528, 2081, 2995
MspI CCGG 6 cut(s) 70, 82, 284, 1898, 3516, 3603
MspR9I CCNGG 5 cut(s) 227, 1848, 1898, 1899, 2156
MunI CAATTG 3 cut(s) 957, 1212, 2752
Mva1269I GAATGC 3 cut(s) 256, 1171, 1470
MvaI CCWGG 3 cut(s) 227, 1848, 2156
MvnI CGCG 2 cut(s) 138, 371
MwoI GCNNNNNNNGC 3 cut(s) 289, 552, 1848
NciI CCSGG 2 cut(s) 1898, 1899
NcoI CCATGG 2 cut(s) 864, 3571
NlaIV GGNNCC 7 cut(s) 1273, 1455, 2636, 2667, 2668, 3300, 3385
NmeAIII GCCGAG 1 cut(s) 2917
NmuCI GTSAC 4 cut(s) 359, 540, 1967, 3592
NsbI TGCGCA 1 cut(s) 2874
NsiI ATGCAT 4 cut(s) 718, 1336, 1788, 2132
NspI RCATGY 3 cut(s) 1414, 1790, 2134
PaeI GCATGC 1 cut(s) 1414
PagI TCATGA 4 cut(s) 643, 1015, 2623, 2947
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 3 cut(s) 256, 1171, 1470
PdmI GAANNNNTTC 4 cut(s) 898, 1011, 1544, 1626
PfeI GAWTC 9 cut(s) 42, 151, 761, 2879, 2951, 3206, 3288, 3427, 3677
PflFI GACNNNGTC 1 cut(s) 365
PkrI GCNGC 6 cut(s) 135, 453, 1381, 2584, 3062, 3135
PleI GAGTC 7 cut(s) 1303, 1439, 1964, 2338, 2832, 2993, 3346
PpsI GAGTC 7 cut(s) 1303, 1439, 1964, 2338, 2832, 2993, 3346
PpuMI RGGWCCY 1 cut(s) 2666
PshBI ATTAAT 1 cut(s) 2367
PsiI TTATAA 1 cut(s) 1602
Psp124BI GAGCTC 1 cut(s) 2163
Psp5II RGGWCCY 1 cut(s) 2666
Psp6I CCWGG 3 cut(s) 225, 1846, 2154
PspEI GGTNACC 1 cut(s) 65
PspGI CCWGG 3 cut(s) 225, 1846, 2154
PspN4I GGNNCC 7 cut(s) 1273, 1455, 2636, 2667, 2668, 3300, 3385
PspOMI GGGCCC 1 cut(s) 1271
PspPI GGNCC 5 cut(s) 944, 1271, 1272, 2635, 2666
PspPPI RGGWCCY 1 cut(s) 2666
PstI CTGCAG 1 cut(s) 1381
PsuI RGATCY 5 cut(s) 1417, 1582, 2722, 3023, 3383
PsyI GACNNNGTC 1 cut(s) 365
PvuII CAGCTG 2 cut(s) 454, 1382
RsaI GTAC 6 cut(s) 459, 1963, 1992, 2778, 3300, 3750
RsaNI GTAC 6 cut(s) 458, 1962, 1991, 2777, 3299, 3749
RseI CAYNNNNRTG 2 cut(s) 618, 2628
SacI GAGCTC 1 cut(s) 2163
SatI GCNGC 6 cut(s) 134, 452, 1380, 2583, 3061, 3134
Sau96I GGNCC 5 cut(s) 944, 1271, 1272, 2635, 2666
ScaI AGTACT 1 cut(s) 2778
SchI GAGTC 7 cut(s) 1304, 1439, 1964, 2338, 2832, 2994, 3347
ScrFI CCNGG 5 cut(s) 227, 1848, 1898, 1899, 2156
SduI GDGCHC 4 cut(s) 222, 1275, 2163, 3616
SfaNI GCATC 7 cut(s) 1050, 1643, 1793, 1851, 2064, 2485, 2569
SfcI CTRYAG 1 cut(s) 1377
SinI GGWCC 3 cut(s) 944, 2635, 2666
SmaI CCCGGG 1 cut(s) 1899
SmiMI CAYNNNNRTG 2 cut(s) 618, 2628
SmlI CTYRAG 7 cut(s) 776, 1427, 1528, 2081, 2995, 3111, 3368
SmoI CTYRAG 7 cut(s) 776, 1427, 1528, 2081, 2995, 3111, 3368
SpeI ACTAGT 1 cut(s) 1309
SphI GCATGC 1 cut(s) 1414
SsiI CCGC 6 cut(s) 133, 146, 371, 1408, 3181, 3476
SspI AATATT 1 cut(s) 187
SstI GAGCTC 1 cut(s) 2163
StyD4I CCNGG 5 cut(s) 225, 1846, 1896, 1897, 2154
StyI CCWWGG 4 cut(s) 864, 1449, 1761, 3571
TaaI ACNGT 3 cut(s) 65, 1914, 3413
TaiI ACGT 3 cut(s) 309, 356, 2571
TaqI TCGA 4 cut(s) 1100, 1548, 2455, 3425
TaqII GACCGA 2 cut(s) 10, 1517
TatI WGTACW 2 cut(s) 1961, 2776
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 9 cut(s) 42, 151, 761, 2879, 2951, 3206, 3288, 3427, 3677
TscAI CASTG 3 cut(s) 227, 261, 3613
TseFI GTSAC 4 cut(s) 359, 540, 1967, 3592
TseI GCWGC 5 cut(s) 451, 1379, 2582, 3060, 3133
Tsp45I GTSAC 4 cut(s) 359, 540, 1967, 3592
TspGWI ACGGA 2 cut(s) 1901, 2978
TspMI CCCGGG 1 cut(s) 1897
TspRI CASTG 3 cut(s) 227, 261, 3613
Tth111I GACNNNGTC 1 cut(s) 365
Vha464I CTTAAG 4 cut(s) 1427, 1528, 2081, 2995
VneI GTGCAC 1 cut(s) 3612
VpaK11BI GGWCC 3 cut(s) 944, 2635, 2666
VspI ATTAAT 1 cut(s) 2367
XagI CCTNNNNNAGG 1 cut(s) 1860
XapI RAATTY 7 cut(s) 666, 1927, 2328, 2735, 2796, 2811, 3631
XceI RCATGY 3 cut(s) 1414, 1790, 2134
XmaI CCCGGG 1 cut(s) 1897
XmiI GTMKAC 1 cut(s) 2588
XmnI GAANNNNTTC 4 cut(s) 898, 1011, 1544, 1626
ZrmI AGTACT 1 cut(s) 2778
Zsp2I ATGCAT 4 cut(s) 718, 1336, 1788, 2132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.