Rh1DG424900

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007218
Physical Location & Seq
Reverse (-)
63845592 .. 63883304
37713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG424900.1

Sequence Viewer

Length: 3270 bp
ATGGATTTGCAAGGCTTATCGGTGATCTGCAACGGCCTCGGAATCGCCCTGGACGACGACGACGGAAACCGGATCGGTTACAGGAAGCACAAGTACTGCTTAGATAATTTGAAGGATTTGCTGAGGTTCTTGAGGCGCGATGATCCGCAGAACCGGGATGTTTTCAAGGAAGTATGCAAGTGGAATATTGTATCCAAGGATCTGATACCTATTATTGAGCACTGCCAAGAGGATCGTAGCTTGGTTTTAAATGCAGTGAAGGTTCTGGTGTTCCTGACTATGCCAGTGGAGCCTACATCAAATGACATTTTACAACAAATTGAATTTCTATGGGAATTGAAGTCTTCAATTACGTCTAGTGACATTGTTGCTGTTATAGTTTCACTTCTGGAAAGTCCACTAGAAAATCTGGAAAGTGATGTGTTCACAGAGGACGACTGGAAATTGGTGCAGCTGGTACTTACTTTATTCCGAAACATTTTAGCCATTCAAGACATTTCATTGCAGCAGAAGTCTGGGGGTACTGCCAGTCAATTTCTATCACGCAGAGATAGCTTTCTGGAACTTTTGTTTCGTGAAAATGTGATGGACTTGGTTTTAGCCATAACACAGCATATTGGTGATCATCGTAGTTATCTCTCTCACGATAACTTGCTTCTATTGGAAATTTTCCATTATACATTTATAGGGCAAGAGCCAGAGTTGATTGCAAATGCATATTCAAAAGGTGCTAAGGTGGAAGAAGATACTATAGATTCTGTAAATAGTCTCAAGTCTATTATGGAGGAAGAGAAAGAGAAAAAGAGACTAAAACTTAGTCGTCATTCCAATTTTGGTGGGACATTTATTCGGCTTACCATGGATGGTTCTAAGGAAGTTGTCAAGGGAAGACCTACTTCTGCTTCTTGTAATACCCTACAGAAATCACAAAATCGTCGAGGTCCAATAAAAAAGATTGCTTGGGATCATGGGATATTACCTTCAACAGAGGATAAGATTTTGGAATTGCTTCATGATTTTGTTAACCAGTTTTTATCAGGGGGTTACAATGTTTTAATGCAGTCAATTCGTCAGGCTATTGAAAAGGAACACCACTCAATCCAGAAGAGTGATGTCATTATCTTCTTTCAGGTTGCTCAGTTTGTTACTTCTTGCCAATATCACAGGCATTCAATTTCTAAGCCAAGCCTGAGAGCTGAAGCAGACACAATTGAAGTTCCCACCGGTAAAGAGGCTGATACAACATTCTTTAGAGGTGATATATGTGGACCCATTGCAACCTCAATGAATGAGTCGATGTTCCAACTAGTCATTTCAAAGTGGCGTTATGCATTCGATGGGTTGAAGGAAACACATGACTACAAGTTTCTGTCTGCAGCTGGTTCTCTTATGAAAGAAATGATTCGCATGCTAGATTTGGTGCTTAAGTTATTGCCAGAAGACTCCAAGGAGCCTCAAACAGCTCGCATACTTCTGTACAAGTTATTTTATGATCAGACCGAGGAGGGGATGACCCATTTTCTCTTAGGGTTGCTCAAATCGTTTGACACTCACAAACAACCAAGAAGTGATCTTGCAGATCTGGTAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCATCTGGAACATTGAGGGTTTCTAAGAAGTCAAGGAAGGGGAGGAAGAGGAGAAAACTGAGTGAAAAGGAAACAGAAAATAAGCTTTCAGGAGAGCATGATACGGTTCAGAAAGAGACTGACATATCCAATGGTGAACAATTAACAGATATGAGTGTAACCGAGAAAAGAAGCCTAGACACAAGTTCTAATGAGAAACAAGACATCAGCATCCCTGGCCAACCTGATGAAGGCAAAAACTCTTTGTTGGAAACAGAAAACTTCTCGAGCAGTCAGGCACAGTTAGATCACAAAAATTCTGCTGATGCAAATGGTGATCTTTGTTATAGTACAGGTGACTCTTCTGGTGATGAGCAAGTAGCTGCAACTACTGAAGTCGATTTTAATGTGTCAAGTTTGGTTTCTGCTTTTTCAAACAACAGCATCATCCAGAAATTGTGTTGGTTGCTTAAATTCTATAAGAGTAACTTGACCAGAACAAACCACTACATAGTATGCATGTTGAGAAGGATCAGTGAAGACCTGGAGCTCTCTCCAATGCTGTATCAGTTATCACTCCTCACTATATTCTATGATATCCTAGCTGAGCAGAATTCATCTCCTTGCAACGAATATGAAAACATTGTTGGTTTCTTGAAAAGTTTGGTCAGGAAAATGCTAAAAAAAATGAAACATCAACCCTTACTTTTTGTGGAAATTCTCTTCTGGAAGACTCGCAAAGAGTGTCATTACATTAATGCTGAATATTTATTGCATGAGCTTGGCCAATTGAGGAATGAAAGTAGAAACTGGGGAGATACTTTAGAGGATGGAGATATGGGTCGATCAATGGACAAGGGATGGACAGCTAGAAGTCTAGCAGATGCACTTGGTGAAGATGAAGCTGATGTTGTGCTCGACTTTGGACATAACAACAATGAAGAAAATCTTGGAAAAGTCAAAGGCGATGCTGCATCTACTTCAGATAATGAGATTGATGAAAGCGGGGGCCAGTCCAAGAAAACTGAAACTGGAAGGGGTCCTCTAAAAAAGAAAAGACTAGTTATTGGAGAGGAACAGGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGAGAACCACAATTGTAGTCACCTAATTGCTGAAGCTCTTGATCCTGATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAACAGCTTGGACTCAAAGTTGCACGAAGGAGAAGATTGCGCCATGCACAGGAATCTGTTTCTGCTGAGCCTAGTCAAACTGATGGAGATGGAGAAGTAGAGGAAACAGTTTTTACTCATCATGAATCAAAAACTCGGCCTTTGCACACAAGAAAAAGAGTCTGTGGCTTAAGTGAAGCACAGGAAGCTCGAATTAGATCTCTCTATGATCAGTTTAAAGACCATAAAAGTTGCAACCACATGATTGCCAATGCAATGGATGGGGATGATAAATTGACAGCTCCTCAAGTTACTCGTAAACTTAAGCAGCTTGGTTTATATATTCCTTGGAAGAAGAGGTCTGGAGCTAACATGCTGATGAGGGATCAGGACCTAAATGATTCCAACAATGCGAATGCCTCTGATGATGAAACATGTTATTTCTGTTGCTGTCTGAATGCCAATCTAGTTTTCAACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1089

Amino Acids

123.91

Weight (kDa)

5.82

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 27 - 282 2.5e-60 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1599
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 146, 2613
AclWI GGATC 9 cut(s) 80, 137, 207, 240, 972, 2147, 2712, 2759, 3180
AcoI YGGCCR 2 cut(s) 1846, 2392
AcsI RAATTY 9 cut(s) 323, 666, 1924, 2081, 2221, 2325, 2717, 2778, 2793
AcuI CTGAAG 4 cut(s) 1218, 2022, 2574, 2775
AdeI CACNNNGTG 1 cut(s) 2501
AfaI GTAC 5 cut(s) 95, 459, 523, 1478, 1960
AfiI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
AflII CTTAAG 3 cut(s) 1424, 2977, 3110
AflIII ACRYGT 1 cut(s) 3221
AgeI ACCGGT 1 cut(s) 1223
AhlI ACTAGT 2 cut(s) 1306, 2668
AjnI CCWGG 3 cut(s) 48, 1843, 2151
AjuI GAANNNNNNNTTGG 5 cut(s) 2238, 2270, 2541, 2573, 3242
Alw21I GWGCWC 3 cut(s) 222, 2160, 2526
Alw26I GTCTC 3 cut(s) 773, 799, 1739
AlwI GGATC 9 cut(s) 80, 137, 207, 240, 972, 2147, 2712, 2759, 3180
Ama87I CYCGRG 1 cut(s) 1894
AoxI GGCC 5 cut(s) 34, 1846, 2392, 2617, 2945
ApeKI GCWGC 6 cut(s) 451, 505, 1376, 1991, 2579, 3115
ApoI RAATTY 9 cut(s) 323, 666, 1924, 2081, 2221, 2325, 2717, 2778, 2793
AseI ATTAAT 1 cut(s) 2364
AsiGI ACCGGT 1 cut(s) 1223
Asp700I GAANNNNTTC 3 cut(s) 1008, 1401, 1623
AspLEI GCGC 2 cut(s) 138, 2850
AspS9I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 9 cut(s) 34, 632, 1268, 1775, 1955, 1976, 1988, 2513, 2735
AvaI CYCGRG 1 cut(s) 1894
AvaII GGWCC 4 cut(s) 941, 1268, 2648, 3178
BalI TGGCCA 2 cut(s) 1848, 2394
BanII GRGCYC 1 cut(s) 2160
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 5 cut(s) 336, 895, 1446, 2154, 2345
Bbv12I GWGCWC 3 cut(s) 222, 2160, 2526
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
BceAI ACGGC 2 cut(s) 49, 1625
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 1049, 1083
BciT130I CCWGG 3 cut(s) 50, 1845, 2153
BciVI GTATCC 1 cut(s) 202
BclI TGATCA 3 cut(s) 622, 1492, 3016
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 3 cut(s) 773, 799, 1739
BcuI ACTAGT 2 cut(s) 1306, 2668
BfmI CTRYAG 3 cut(s) 750, 917, 1374
BfrI CTTAAG 3 cut(s) 1424, 2977, 3110
BfuI GTATCC 1 cut(s) 202
BglII AGATCT 2 cut(s) 1579, 3005
BisI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
BlpI GCTNAGC 2 cut(s) 2214, 2874
BlsI GCNGC 6 cut(s) 453, 507, 1378, 1993, 2581, 3117
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 4 cut(s) 50, 155, 1845, 2153
Bme18I GGWCC 4 cut(s) 941, 1268, 2648, 3178
BmeT110I CYCGRG 1 cut(s) 1894
BmgT120I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
BmiI GGNNCC 5 cut(s) 291, 1270, 1452, 2618, 2649
BmrFI CCNGG 4 cut(s) 50, 155, 1845, 2153
BmrI ACTGGG 1 cut(s) 2428
BmsI GCATC 7 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591
BmuI ACTGGG 1 cut(s) 2428
BpiI GAAGAC 5 cut(s) 336, 895, 1446, 2154, 2345
BpmI CTGGAG 2 cut(s) 2174, 3171
Bpu10I CCTNAGC 2 cut(s) 122, 732
Bpu1102I GCTNAGC 2 cut(s) 2214, 2874
BpuEI CTTGAG 3 cut(s) 151, 755, 3078
BpuMI CCSGG 1 cut(s) 155
BsaJI CCNNGG 8 cut(s) 37, 48, 195, 858, 1446, 1500, 1843, 3134
BsaWI WCCGGW 2 cut(s) 69, 1223
Bsc4I CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
Bse118I RCCGGY 1 cut(s) 1223
Bse1I ACTGG 7 cut(s) 284, 443, 528, 1027, 2423, 2620, 2644
Bse3DI GCAATG 3 cut(s) 500, 1272, 3069
BseBI CCWGG 3 cut(s) 50, 1845, 2153
BseDI CCNNGG 8 cut(s) 37, 48, 195, 858, 1446, 1500, 1843, 3134
BseGI GGATG 9 cut(s) 163, 868, 1515, 1839, 2055, 2443, 2474, 3073, 3079
BseLI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
BseMI GCAATG 3 cut(s) 500, 1272, 3069
BseMII CTCAG 6 cut(s) 113, 1151, 1181, 1679, 2205, 2865
BseNI ACTGG 7 cut(s) 284, 443, 528, 1027, 2423, 2620, 2644
BseRI GAGGAG 4 cut(s) 1517, 1693, 2177, 3081
BseXI GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BshTI ACCGGT 1 cut(s) 1223
BsiHKAI GWGCWC 3 cut(s) 222, 2160, 2526
BsiHKCI CYCGRG 1 cut(s) 1894
BsiSI CCGG 3 cut(s) 70, 154, 1224
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
BsmAI GTCTC 3 cut(s) 773, 799, 1739
BsmFI GGGAC 1 cut(s) 853
BsmI GAATGC 4 cut(s) 1168, 1331, 3208, 3250
BsnI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BsoBI CYCGRG 1 cut(s) 1894
Bsp1286I GDGCHC 3 cut(s) 222, 2160, 2526
Bsp1407I TGTACA 1 cut(s) 1476
Bsp1720I GCTNAGC 2 cut(s) 2214, 2874
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 2 cut(s) 146, 2613
BspANI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BspCNI CTCAG 6 cut(s) 114, 1150, 1182, 1680, 2206, 2866
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 2 cut(s) 1012, 2929
BspLI GGNNCC 5 cut(s) 291, 1270, 1452, 2618, 2649
BspMAI CTGCAG 1 cut(s) 1378
BspPI GGATC 9 cut(s) 80, 137, 207, 240, 972, 2147, 2712, 2759, 3180
BspTI CTTAAG 3 cut(s) 1424, 2977, 3110
BsrDI GCAATG 3 cut(s) 500, 1272, 3069
BsrFI RCCGGY 1 cut(s) 1223
BsrGI TGTACA 1 cut(s) 1476
BsrI ACTGG 7 cut(s) 284, 443, 528, 1027, 2423, 2620, 2644
BssAI RCCGGY 1 cut(s) 1223
BssECI CCNNGG 8 cut(s) 37, 48, 195, 858, 1446, 1500, 1843, 3134
BssT1I CCWWGG 4 cut(s) 195, 858, 1446, 3134
Bst2UI CCWGG 3 cut(s) 50, 1845, 2153
Bst4CI ACNGT 3 cut(s) 1735, 1911, 2917
Bst6I CTCTTC 6 cut(s) 783, 1100, 1670, 1975, 2336, 3137
BstAFI CTTAAG 3 cut(s) 1424, 2977, 3110
BstAUI TGTACA 1 cut(s) 1476
BstC8I GCNNGC 2 cut(s) 1409, 1465
BstDSI CCRYGG 1 cut(s) 858
BstENI CCTNNNNNAGG 1 cut(s) 1857
BstF5I GGATG 9 cut(s) 163, 868, 1515, 1839, 2055, 2443, 2474, 3073, 3079
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 2 cut(s) 138, 2850
BstMAI GTCTC 3 cut(s) 773, 799, 1739
BstMWI GCNNNNNNNGC 5 cut(s) 289, 552, 1845, 2812, 2993
BstNI CCWGG 3 cut(s) 50, 1845, 2153
BstNSI RCATGY 4 cut(s) 1411, 2131, 3163, 3225
BstSCI CCNGG 4 cut(s) 48, 153, 1843, 2151
BstSFI CTRYAG 3 cut(s) 750, 917, 1374
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
BstV2I GAAGAC 5 cut(s) 336, 895, 1446, 2154, 2345
BstX2I RGATCY 4 cut(s) 199, 1579, 2704, 3005
BstXI CCANNNNNNTGG 1 cut(s) 3064
BstYI RGATCY 4 cut(s) 199, 1579, 2704, 3005
BsuI GTATCC 1 cut(s) 202
BsuRI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BtgI CCRYGG 1 cut(s) 858
BtgZI GCGATG 2 cut(s) 153, 2589
BtsCI GGATG 9 cut(s) 163, 868, 1515, 1839, 2055, 2443, 2474, 3073, 3079
BtsI GCAGTG 2 cut(s) 220, 261
BtsIMutI CAGTG 4 cut(s) 220, 261, 291, 2149
Cac8I GCNNGC 2 cut(s) 1409, 1465
CciI TCATGA 2 cut(s) 1012, 2929
CfoI GCGC 2 cut(s) 138, 2850
Cfr10I RCCGGY 1 cut(s) 1223
Cfr13I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
Csp6I GTAC 5 cut(s) 94, 458, 522, 1477, 1959
CspAI ACCGGT 1 cut(s) 1223
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviQI GTAC 5 cut(s) 94, 458, 522, 1477, 1959
DraI TTTAAA 2 cut(s) 249, 3025
DraIII CACNNNGTG 1 cut(s) 2501
EaeI YGGCCR 2 cut(s) 1846, 2392
Eam1104I CTCTTC 6 cut(s) 783, 1100, 1670, 1975, 2336, 3137
EarI CTCTTC 6 cut(s) 783, 1100, 1670, 1975, 2336, 3137
Ecl136II GAGCTC 1 cut(s) 2158
Eco130I CCWWGG 4 cut(s) 195, 858, 1446, 3134
Eco24I GRGCYC 1 cut(s) 2160
Eco32I GATATC 1 cut(s) 2206
Eco47I GGWCC 4 cut(s) 941, 1268, 2648, 3178
Eco53kI GAGCTC 1 cut(s) 2158
Eco57I CTGAAG 4 cut(s) 1218, 2022, 2574, 2775
Eco88I CYCGRG 1 cut(s) 1894
EcoICRI GAGCTC 1 cut(s) 2158
EcoNI CCTNNNNNAGG 1 cut(s) 1857
EcoO109I RGGNCCY 2 cut(s) 2648, 3178
EcoRI GAATTC 1 cut(s) 2221
EcoRII CCWGG 3 cut(s) 48, 1843, 2151
EcoRV GATATC 1 cut(s) 2206
EcoT14I CCWWGG 4 cut(s) 195, 858, 1446, 3134
EcoT22I ATGCAT 3 cut(s) 718, 1333, 2129
EcoT38I GRGCYC 1 cut(s) 2160
ErhI CCWWGG 4 cut(s) 195, 858, 1446, 3134
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 880, 912, 2081, 2113, 2541, 2573
FaqI GGGAC 1 cut(s) 853
FauI CCCGC 1 cut(s) 2606
FbaI TGATCA 3 cut(s) 622, 1492, 3016
Fnu4HI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
FokI GGATG 9 cut(s) 170, 875, 1522, 1826, 2042, 2450, 2481, 3080, 3086
FriOI GRGCYC 1 cut(s) 2160
Fsp4HI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
GlaI GCGC 2 cut(s) 137, 2849
GluI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
GsuI CTGGAG 2 cut(s) 2174, 3171
HaeIII GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
HapII CCGG 3 cut(s) 70, 154, 1224
HhaI GCGC 2 cut(s) 138, 2850
Hin6I GCGC 2 cut(s) 136, 2848
HinP1I GCGC 2 cut(s) 136, 2848
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 2 cut(s) 1712, 2804
HpaI GTTAAC 1 cut(s) 1024
HpaII CCGG 3 cut(s) 70, 154, 1224
HphI GGTGA 9 cut(s) 34, 632, 1268, 1775, 1955, 1976, 1988, 2513, 2735
Hpy166II GTNNAC 6 cut(s) 398, 426, 1024, 1268, 1766, 3107
Hpy188I TCNGA 8 cut(s) 41, 204, 473, 1497, 1740, 2593, 3211, 3243
Hpy8I GTNNAC 6 cut(s) 398, 426, 1024, 1268, 1766, 3107
Hpy99I CGWCG 4 cut(s) 59, 62, 65, 939
HpyCH4III ACNGT 3 cut(s) 1735, 1911, 2917
HpyCH4IV ACGT 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 5 cut(s) 289, 552, 1845, 2812, 2993
HpySE526I ACGT 1 cut(s) 353
HspAI GCGC 2 cut(s) 136, 2848
Ksp22I TGATCA 3 cut(s) 622, 1492, 3016
KspAI GTTAAC 1 cut(s) 1024
LmnI GCTCC 5 cut(s) 289, 1450, 2155, 3094, 3152
Lsp1109I GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
LweI GCATC 7 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 9 cut(s) 77, 359, 1043, 1144, 1786, 1964, 2093, 2741, 3097
MfeI CAATTG 3 cut(s) 1209, 2396, 2734
MflI RGATCY 4 cut(s) 199, 1579, 2704, 3005
MhlI GDGCHC 3 cut(s) 222, 2160, 2526
MlsI TGGCCA 2 cut(s) 1848, 2394
MluNI TGGCCA 2 cut(s) 1848, 2394
MlyI GAGTC 6 cut(s) 1301, 1436, 1961, 2335, 2814, 2976
MmeI TCCRAC 4 cut(s) 1327, 1857, 2823, 3216
Mox20I TGGCCA 2 cut(s) 1848, 2394
Mph1103I ATGCAT 3 cut(s) 718, 1333, 2129
MroXI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
MscI TGGCCA 2 cut(s) 1848, 2394
MslI CAYNNNNRTG 2 cut(s) 618, 3164
Msp20I TGGCCA 2 cut(s) 1848, 2394
MspA1I CMGCKG 2 cut(s) 454, 1379
MspCI CTTAAG 3 cut(s) 1424, 2977, 3110
MspI CCGG 3 cut(s) 70, 154, 1224
MspR9I CCNGG 4 cut(s) 50, 155, 1845, 2153
MunI CAATTG 3 cut(s) 1209, 2396, 2734
Mva1269I GAATGC 4 cut(s) 1168, 1331, 3208, 3250
MvaI CCWGG 3 cut(s) 50, 1845, 2153
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 5 cut(s) 289, 552, 1845, 2812, 2993
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 858
NlaIV GGNNCC 5 cut(s) 291, 1270, 1452, 2618, 2649
NmeAIII GCCGAG 1 cut(s) 2923
NmuCI GTSAC 3 cut(s) 359, 1964, 2741
NsiI ATGCAT 3 cut(s) 718, 1333, 2129
NspI RCATGY 4 cut(s) 1411, 2131, 3163, 3225
PaeI GCATGC 1 cut(s) 1411
PaeR7I CTCGAG 1 cut(s) 1894
PagI TCATGA 2 cut(s) 1012, 2929
PciI ACATGT 1 cut(s) 3221
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 4 cut(s) 1168, 1331, 3208, 3250
PdmI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
PfeI GAWTC 6 cut(s) 42, 755, 1402, 2861, 2933, 3188
PinAI ACCGGT 1 cut(s) 1223
PkrI GCNGC 6 cut(s) 453, 507, 1378, 1993, 2581, 3117
PleI GAGTC 6 cut(s) 1300, 1436, 1961, 2335, 2814, 2975
PpsI GAGTC 6 cut(s) 1300, 1436, 1961, 2335, 2814, 2975
PpuMI RGGWCCY 2 cut(s) 2648, 3178
PscI ACATGT 1 cut(s) 3221
PshBI ATTAAT 1 cut(s) 2364
PsiI TTATAA 1 cut(s) 1599
Psp124BI GAGCTC 1 cut(s) 2160
Psp5II RGGWCCY 2 cut(s) 2648, 3178
Psp6I CCWGG 3 cut(s) 48, 1843, 2151
PspGI CCWGG 3 cut(s) 48, 1843, 2151
PspN4I GGNNCC 5 cut(s) 291, 1270, 1452, 2618, 2649
PspPI GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
PspPPI RGGWCCY 2 cut(s) 2648, 3178
PstI CTGCAG 1 cut(s) 1378
PsuI RGATCY 4 cut(s) 199, 1579, 2704, 3005
PvuII CAGCTG 2 cut(s) 454, 1379
RsaI GTAC 5 cut(s) 95, 459, 523, 1478, 1960
RsaNI GTAC 5 cut(s) 94, 458, 522, 1477, 1959
RseI CAYNNNNRTG 2 cut(s) 618, 3164
SacI GAGCTC 1 cut(s) 2160
SatI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
Sau96I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 6 cut(s) 1301, 1436, 1961, 2335, 2814, 2976
ScrFI CCNGG 4 cut(s) 50, 155, 1845, 2153
SduI GDGCHC 3 cut(s) 222, 2160, 2526
SfaNI GCATC 7 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591
SfcI CTRYAG 3 cut(s) 750, 917, 1374
Sfr274I CTCGAG 1 cut(s) 1894
SinI GGWCC 4 cut(s) 941, 1268, 2648, 3178
SlaI CTCGAG 1 cut(s) 1894
SmiMI CAYNNNNRTG 2 cut(s) 618, 3164
SmlI CTYRAG 7 cut(s) 130, 770, 1424, 1894, 2977, 3093, 3110
SmoI CTYRAG 7 cut(s) 130, 770, 1424, 1894, 2977, 3093, 3110
SpeI ACTAGT 2 cut(s) 1306, 2668
SphI GCATGC 1 cut(s) 1411
SsiI CCGC 2 cut(s) 146, 2613
SspI AATATT 2 cut(s) 187, 2375
SstI GAGCTC 1 cut(s) 2160
StyD4I CCNGG 4 cut(s) 48, 153, 1843, 2151
StyI CCWWGG 4 cut(s) 195, 858, 1446, 3134
TaaI ACNGT 3 cut(s) 1735, 1911, 2917
TaiI ACGT 1 cut(s) 356
TaqI TCGA 8 cut(s) 937, 1295, 1335, 1895, 2007, 2452, 2526, 2998
TaqII GACCGA 1 cut(s) 1514
TatI WGTACW 3 cut(s) 93, 1476, 1958
TfiI GAWTC 6 cut(s) 42, 755, 1402, 2861, 2933, 3188
TscAI CASTG 4 cut(s) 227, 261, 291, 2149
TseFI GTSAC 3 cut(s) 359, 1964, 2741
TseI GCWGC 6 cut(s) 451, 505, 1376, 1991, 2579, 3115
Tsp45I GTSAC 3 cut(s) 359, 1964, 2741
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 4 cut(s) 227, 261, 291, 2149
Vha464I CTTAAG 3 cut(s) 1424, 2977, 3110
VpaK11BI GGWCC 4 cut(s) 941, 1268, 2648, 3178
VspI ATTAAT 1 cut(s) 2364
XagI CCTNNNNNAGG 1 cut(s) 1857
XapI RAATTY 9 cut(s) 323, 666, 1924, 2081, 2221, 2325, 2717, 2778, 2793
XceI RCATGY 4 cut(s) 1411, 2131, 3163, 3225
XhoI CTCGAG 1 cut(s) 1894
XmnI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
ZrmI AGTACT 1 cut(s) 95
Zsp2I ATGCAT 3 cut(s) 718, 1333, 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.