Rroxscaffold_4G00279950

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007218
Physical Location & Seq
Forward (+)
2594405 .. 2610867
16463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279950.1

Sequence Viewer

Length: 3891 bp
ATGGCTAATGTTTACATTGGTGATGATGCTGAGACATTCGCATTCCACGTCGAGATCAACCAGCTTCTTAGATTGATCATCAATACTTTTTACAGCAACAAAGAAATTTTCCTTGGCCAACTTATCAACAATTCCAATGATCGGTATCGGCATGACCAAAGCAGATCATTAGCTGTTAGTTGTGTTCAGTTGGAATACTTGGAAGAGATGAGGATCAAAGACCTTGAGATTACCAAGGATGAGTATGCTTTATTCTACAAGAGCTTGACCAATGATTGGGAGGACTACCTTGCAGATGTTCAGAAGCGAGTCAGAAGAGCAAATAGGCAATTAAGTATTGTGATTCATTTTCTGTGCAGGAAACGAACCTTGGGCAAAGATTACATATTAGAGTTACAATTTTATAGCCACAAGTACTGCTTAGAGTTACATTTCTTGAAGGTTCTGGTGTTCCTGACCATGCCAGTTGAGCCTACATCAAATGACATTTTACAACAAATTGAATTTCTATGGGAGTTGAAGTCTTCAATTACGTCTAGTGACATTGTTGCTGTTATAGTTTCACTTCTGGAAAGTCCACTAGAAAATCTGGAAAGTGATGTGTTCACAGAGGACGACTGGAAATTGGTGCAGTTGGTACTTACTTTATTCCGAAACATTTTAGCCATTCAAGACATTTCATTGCAGCAGAAGTCTGGGGGTGCTGCCAGTCAATTTCTATCACGCAGAGATAGCTTTCTGGAACTTTTGTTTCGTGAAAATGTGATGGACTTGGTTTTAGCCATAACACAGCATATTGGTGATCATCGTAGTTATCTCTCTCATGATAACTTGCTTCTATTGGAAATTTTCCATTATACATTTATAGGGCAGGAGCCAGAGTTGATTTCAAATGCATATTCAAAAGGTGCTAAGGTGGAAGAAGATACTATAGATTCTGTAAATAGTCTCAAGTCTATTATGGAGGAGGAGAAAGAGAAAAAGAGACTAAAAGTTAGTCGTCATTCCAATTTTGGTGGGACATTTATTCGGCTTACCATGGATGGTTCTAAGGAAGTTGTCAAGGGAAGACCTACTTCTGCTTCTTGTAATACCCTACAGAAATCACAAAATCGTCGAGGTCCAATAAAAAAGATTGCTTGGGATCATGGGATATTACCTTCAACAGAGGATAAGATTTTGGAATTGCTTCATGATTTTGTTAACCAGTTTTTATCAGGGGGTTACAATGTTTTAATGCAGTCAATTCGTCAGGCTATTGAAAAGGAACACCACTCAATCCAGAAGAGCGATGTCATTATCTTCTTTCAGGTTGCTCAGTTTGTTACTTCTTTCCAATATCACAGGCATTCAATTTTTAAGCCAAGCCTGAGAGCTGAAGCAGACACAATTGAAGTTCCCACTGGTAAAGAGGCTGATAGAACATTCTTTAGAGGTGATATATGTGGACCCATTGCAACCTCAATGAATGAGTCGATGTTCCAACTAGTCATTTCAAAGTGGCGTTATGCATTTGATGGGTTGAAGGAAACACATGACTACAAGTTTCTGTCTGCAGCTGGTTCTCTAATGAAAGAAATGATTCGCATGCTAGATTTGGTGCTTAAGTTGTTGCCAGAAGACTCCAAGGAGCCTCAAACAGCTCGCATACTTCTGTACAAGTTATTTTATGATCAGACCGAAGAGGGGATGACCCATTTTCTCTTAGGGTTGCTGAAATCGTTTGACACTCACAAACAACCAAGAAGTGATCTTGCAGATCTGGTAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCAGCTGGAACATTGAGGGTTTCTAAGAAGTCAAGGAAGGGGAGGAACAGGAGAAAACTGAGTGAAAAGGAAACAGAAAATAAGCTCTCAGGAGAGCATGATACGGTTCAGAAAGACACTGACATCTCCAATGGTGAACAATCAACAGATATGAGTGTAACTGAGAAAAGAAGCCTAGACACAAGTTCTAATGAGAAACAAGACATCAGCATCCCTGGCCAACCTGATGAGGGCAAAAACTCTTTGTTGGAGACAGAAAACTTCTCAGGCAGTCAGGCACAGTTAGATCACAAAAATTCTGGTGATGCAAATGGTGATCTTTGTTATAGTACAGGTGACTCTTCTGGTGATGAGCAAGTAGCTGCAACTACTGAAGTCGATTTTAAGGTGTCAAGTTTGGTTTCTGCCTTTTCAAACAACAGCATCATCCAGAAATTGTGTTGGTTGCTTAAGTTCTATAAGAGTAACTTGACCAGAACAAACCACTACATAGTATGCATGTTGAGAAGGATCAGTGAAGACCTGGAGCTCTCTCCAATGCTGTATCAGTTATCACTCCTCACTATATTCTATGATATCCTAGCTGAGCAGAATTCATCTCCTTGCAACGAATATGAAAACATTGTTGGTTTCTTGAAAAGTTTGGTCAGGAAAATGCTAAAAAAAATGAAACATCAACCCTTACTTTTTGTGGAAATTCTCTTCTGGAAGACTCGCAAAGAGTGTCATTACATTAATGCTGAATATTTATTGCATGAGCTTGGCCAATTGAGGAATGAAAGTAGAAACTGGGGAGATACTTTAGAGGATGGAGATATGGGTCGATCAATGGACAAGGGATGGACAGGTAGAAGTCTAGCAGATGCACTTGGTGAAGACGAAGCTGATGTTGTGCTCGACTTCGGACATAACAACAATGAAGAAAATCTTGGAAAAGTGAAAGGCGATGCTGCATCTACTTCAGATAATGAGATTGATGAAAGACTAGTTATTGGAGAGGAACAGGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGAGAACCACAATTGTAGTCACCTAATTGCTGAAGCTCTTGATCCTGATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAACAGCTTGGACTCAAAGTTGCACGAAGGAGAAGATTGCGCCATGCACAGGAATCTGTTTCTGCTGAGCCTAGTCAAACTGATGGAGATGGAGAAGTAGAGGAAACAGTTATTACTCATCATGAATCAAAAACTCGGCCTTTGCACACAAGAAAAAGAGTCCGTGGCTTAAGCGAAGAACAGGAAGCTCAAATTAGATCTCTCTATGATCAGTTTAAAGACCATAAAAGTTGCAACCACATGATTGCCAATGCAATGGATGGGGATGATAAATTGACAGCTCCTCAAGTTTCTCGTAAACTTAAGCAGCTTGGGTTATGTATTCCTCGGAAGAAGAGGTCTGGAGCTAACATGCCGATGAGGGATCAGGACCTAAATGATTCTGACACAAACGATGCCAATGCCTCTGATGATGAAACATTGTTATCATTAATGAACAGGGGCAAAAAGGATAATATCAGAATTAGTGAGGTACCAGAACAGATTACCAGAAGAGAAGTGTCAGAAGAGGAGTCTGATGATGAAATGCTAAGCTCTGTTCTCAAGAAAGCTAAGGGATCCCTTTCTAAATTAAAGGATCAAAACCTCACAGCCATCTCGATTCAGGGAACAGCAAGAGGAAATGCTTTAGAAAATAAGGTTGAAGAAGCTGTTGTATCTTCTGGCTGGGATGTTAACGAGCAATCCTCTGGGTTGGGACATGCCAAAATTCACGATGTAGATACTTTCAATCATGCGAGTCCCAATGGCACTGCACAAGCTGAAGTCACAAGCACTGGCAGTGGTAATCTATTAGATGCTACACCTGTGAAAAATGTAGAGGATTTGGAACATCAGAAAATTGGCATTGACTTGGAAGATTCAGGGGATGAAGCAACTCATAGTGCATTAATACAGGGTGGTGCGAGTAGAAGGAAGATGAGGATGATGGTGCTCGAAGATGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1296

Amino Acids

147.3

Weight (kDa)

5.47

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 91 - 342 1.2e-39 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1779
Acc65I GGTACC 1 cut(s) 3406
AccB1I GGYRCC 1 cut(s) 3406
AccB7I CCANNNNNTGG 1 cut(s) 276
AclWI GGATC 9 cut(s) 221, 1152, 2327, 2838, 2885, 3306, 3486, 3499, 3519
AcoI YGGCCR 3 cut(s) 115, 2026, 2572
AcuI CTGAAG 5 cut(s) 1398, 2202, 2754, 2901, 3717
AdeI CACNNNGTG 1 cut(s) 2681
AfaI GTAC 5 cut(s) 416, 639, 1658, 2140, 3408
AfiI CCNNNNNNNGG 5 cut(s) 141, 276, 1686, 2039, 2983
AflII CTTAAG 4 cut(s) 1604, 2258, 3103, 3236
AhlI ACTAGT 2 cut(s) 1486, 2794
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 2 cut(s) 2023, 2331
AjuI GAANNNNNNNTTGG 8 cut(s) 96, 128, 353, 385, 2418, 2450, 2721, 2753
Alw21I GWGCWC 3 cut(s) 2340, 2706, 3870
Alw26I GTCTC 4 cut(s) 26, 953, 979, 2054
AlwI GGATC 9 cut(s) 221, 1152, 2327, 2838, 2885, 3306, 3486, 3499, 3519
AoxI GGCC 4 cut(s) 115, 2026, 2572, 3071
ApeKI GCWGC 7 cut(s) 685, 704, 1556, 1811, 2171, 2759, 3241
AseI ATTAAT 3 cut(s) 2544, 3365, 3824
Asp700I GAANNNNTTC 3 cut(s) 1188, 1581, 1803
Asp718I GGTACC 1 cut(s) 3406
AspLEI GCGC 1 cut(s) 2976
AspS9I GGNCC 3 cut(s) 1121, 1448, 3304
AvaII GGWCC 3 cut(s) 1121, 1448, 3304
BalI TGGCCA 3 cut(s) 117, 2028, 2574
BamHI GGATCC 1 cut(s) 3491
BanI GGYRCC 1 cut(s) 3406
BanII GRGCYC 1 cut(s) 2340
BbsI GAAGAC 6 cut(s) 516, 1075, 1626, 2334, 2525, 2691
Bbv12I GWGCWC 3 cut(s) 2340, 2706, 3870
BbvI GCAGC 7 cut(s) 691, 697, 1568, 1823, 2158, 2746, 3253
BceAI ACGGC 1 cut(s) 1805
BcgI CGANNNNNNTGC 4 cut(s) 1229, 1263, 3317, 3351
BciT130I CCWGG 2 cut(s) 2025, 2333
BclI TGATCA 4 cut(s) 75, 802, 1672, 3142
BcoDI GTCTC 4 cut(s) 26, 953, 979, 2054
BcuI ACTAGT 2 cut(s) 1486, 2794
BfaI CTAG 9 cut(s) 537, 581, 1487, 1592, 1985, 2390, 2666, 2795, 3006
BfmI CTRYAG 3 cut(s) 930, 1097, 1554
BfrI CTTAAG 4 cut(s) 1604, 2258, 3103, 3236
BglII AGATCT 2 cut(s) 1759, 3131
BisI GCNGC 7 cut(s) 686, 705, 1557, 1812, 2172, 2760, 3242
BlpI GCTNAGC 3 cut(s) 2394, 3000, 3464
BlsI GCNGC 7 cut(s) 687, 706, 1558, 1813, 2173, 2761, 3243
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 2 cut(s) 2025, 2333
Bme18I GGWCC 3 cut(s) 1121, 1448, 3304
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 3 cut(s) 1121, 1448, 3304
BmiI GGNNCC 5 cut(s) 876, 1450, 1632, 3408, 3493
BmrFI CCNGG 2 cut(s) 2025, 2333
BmrI ACTGGG 1 cut(s) 2608
BmsI GCATC 9 cut(s) 16, 2028, 2104, 2241, 2662, 2746, 2771, 3319, 3721
BmuI ACTGGG 1 cut(s) 2608
BpiI GAAGAC 6 cut(s) 516, 1075, 1626, 2334, 2525, 2691
BplI GAGNNNNNCTC 2 cut(s) 3605, 3637
BpmI CTGGAG 2 cut(s) 2354, 3297
Bpu10I CCTNAGC 2 cut(s) 912, 3486
Bpu1102I GCTNAGC 3 cut(s) 2394, 3000, 3464
BpuEI CTTGAG 4 cut(s) 245, 935, 3204, 3461
BsaBI GATNNNNATC 2 cut(s) 144, 212
BsaJI CCNNGG 8 cut(s) 112, 234, 369, 1038, 1626, 2023, 3097, 3260
BsaXI ACNNNNNCTCC 4 cut(s) 506, 536, 866, 896
Bsc4I CCNNNNNNNGG 5 cut(s) 141, 276, 1686, 2039, 2983
Bse1I ACTGG 7 cut(s) 464, 623, 708, 1207, 1408, 2603, 3715
Bse3DI GCAATG 3 cut(s) 680, 1452, 3195
Bse8I GATNNNNATC 2 cut(s) 144, 212
BseBI CCWGG 2 cut(s) 2025, 2333
BseDI CCNNGG 8 cut(s) 112, 234, 369, 1038, 1626, 2023, 3097, 3260
BseJI GATNNNNATC 2 cut(s) 144, 212
BseLI CCNNNNNNNGG 5 cut(s) 141, 276, 1686, 2039, 2983
BseMI GCAATG 3 cut(s) 680, 1452, 3195
BseMII CTCAG 9 cut(s) 21, 1331, 1361, 1859, 1911, 1962, 2088, 2385, 2991
BseNI ACTGG 7 cut(s) 464, 623, 708, 1207, 1408, 2603, 3715
BseRI GAGGAG 5 cut(s) 980, 983, 2357, 3207, 3458
BseXI GCAGC 7 cut(s) 691, 697, 1568, 1823, 2158, 2746, 3253
BseYI CCCAGC 1 cut(s) 3600
BsgI GTGCAG 3 cut(s) 376, 650, 3672
BshFI GGCC 4 cut(s) 117, 2028, 2574, 3073
BshNI GGYRCC 1 cut(s) 3406
BsiHKAI GWGCWC 3 cut(s) 2340, 2706, 3870
BslFI GGGAC 3 cut(s) 1033, 3645, 3660
BslI CCNNNNNNNGG 5 cut(s) 141, 276, 1686, 2039, 2983
BsmAI GTCTC 4 cut(s) 26, 953, 979, 2054
BsmFI GGGAC 3 cut(s) 1033, 3645, 3660
BsmI GAATGC 2 cut(s) 41, 1348
BsnI GGCC 4 cut(s) 117, 2028, 2574, 3073
Bsp1286I GDGCHC 3 cut(s) 2340, 2706, 3870
Bsp1407I TGTACA 1 cut(s) 1656
Bsp1720I GCTNAGC 3 cut(s) 2394, 3000, 3464
Bsp19I CCATGG 1 cut(s) 1038
BspANI GGCC 4 cut(s) 117, 2028, 2574, 3073
BspCNI CTCAG 9 cut(s) 22, 1330, 1362, 1860, 1910, 1963, 2087, 2386, 2992
BspHI TCATGA 3 cut(s) 823, 1192, 3055
BspLI GGNNCC 5 cut(s) 876, 1450, 1632, 3408, 3493
BspMAI CTGCAG 1 cut(s) 1558
BspPI GGATC 9 cut(s) 221, 1152, 2327, 2838, 2885, 3306, 3486, 3499, 3519
BspQI GCTCTTC 2 cut(s) 310, 1280
BspT107I GGYRCC 1 cut(s) 3406
BspTI CTTAAG 4 cut(s) 1604, 2258, 3103, 3236
BsrDI GCAATG 3 cut(s) 680, 1452, 3195
BsrGI TGTACA 1 cut(s) 1656
BsrI ACTGG 7 cut(s) 464, 623, 708, 1207, 1408, 2603, 3715
BssECI CCNNGG 8 cut(s) 112, 234, 369, 1038, 1626, 2023, 3097, 3260
BssT1I CCWWGG 5 cut(s) 112, 234, 369, 1038, 1626
Bst2UI CCWGG 2 cut(s) 2025, 2333
Bst4CI ACNGT 3 cut(s) 1915, 2091, 3043
Bst6I CTCTTC 9 cut(s) 198, 310, 1280, 1677, 2155, 2516, 3263, 3421, 3435
BstAFI CTTAAG 4 cut(s) 1604, 2258, 3103, 3236
BstAUI TGTACA 1 cut(s) 1656
BstC8I GCNNGC 2 cut(s) 1589, 1645
BstDSI CCRYGG 2 cut(s) 1038, 3097
BstHHI GCGC 1 cut(s) 2976
BstMAI GTCTC 4 cut(s) 26, 953, 979, 2054
BstMWI GCNNNNNNNGC 4 cut(s) 469, 732, 2025, 2938
BstNI CCWGG 2 cut(s) 2025, 2333
BstNSI RCATGY 4 cut(s) 1591, 2311, 3289, 3638
BstSCI CCNGG 2 cut(s) 2023, 2331
BstSFI CTRYAG 3 cut(s) 930, 1097, 1554
BstV1I GCAGC 7 cut(s) 691, 697, 1568, 1823, 2158, 2746, 3253
BstV2I GAAGAC 6 cut(s) 516, 1075, 1626, 2334, 2525, 2691
BstX2I RGATCY 4 cut(s) 1759, 2830, 3131, 3491
BstXI CCANNNNNNTGG 1 cut(s) 3190
BstYI RGATCY 4 cut(s) 1759, 2830, 3131, 3491
BsuRI GGCC 4 cut(s) 117, 2028, 2574, 3073
BtgI CCRYGG 2 cut(s) 1038, 3097
BtgZI GCGATG 2 cut(s) 1305, 2769
BtrI CACGTC 1 cut(s) 49
BtsI GCAGTG 2 cut(s) 3684, 3721
BtsIMutI CAGTG 6 cut(s) 1401, 1926, 2329, 3684, 3708, 3721
Cac8I GCNNGC 2 cut(s) 1589, 1645
CciI TCATGA 3 cut(s) 823, 1192, 3055
CfoI GCGC 1 cut(s) 2976
Cfr13I GGNCC 3 cut(s) 1121, 1448, 3304
Csp6I GTAC 5 cut(s) 415, 638, 1657, 2139, 3407
CspCI CAANNNNNGTGG 2 cut(s) 997, 1032
CviQI GTAC 5 cut(s) 415, 638, 1657, 2139, 3407
DraI TTTAAA 1 cut(s) 3151
DraIII CACNNNGTG 1 cut(s) 2681
EaeI YGGCCR 3 cut(s) 115, 2026, 2572
Eam1104I CTCTTC 9 cut(s) 198, 310, 1280, 1677, 2155, 2516, 3263, 3421, 3435
EarI CTCTTC 9 cut(s) 198, 310, 1280, 1677, 2155, 2516, 3263, 3421, 3435
Ecl136II GAGCTC 1 cut(s) 2338
Eco130I CCWWGG 5 cut(s) 112, 234, 369, 1038, 1626
Eco24I GRGCYC 1 cut(s) 2340
Eco32I GATATC 1 cut(s) 2386
Eco47I GGWCC 3 cut(s) 1121, 1448, 3304
Eco53kI GAGCTC 1 cut(s) 2338
Eco57I CTGAAG 5 cut(s) 1398, 2202, 2754, 2901, 3717
EcoICRI GAGCTC 1 cut(s) 2338
EcoO109I RGGNCCY 1 cut(s) 3304
EcoRI GAATTC 1 cut(s) 2401
EcoRII CCWGG 2 cut(s) 2023, 2331
EcoRV GATATC 1 cut(s) 2386
EcoT14I CCWWGG 5 cut(s) 112, 234, 369, 1038, 1626
EcoT22I ATGCAT 3 cut(s) 898, 1513, 2309
EcoT38I GRGCYC 1 cut(s) 2340
ErhI CCWWGG 5 cut(s) 112, 234, 369, 1038, 1626
FalI AAGNNNNNCTT 8 cut(s) 404, 436, 1060, 1092, 2261, 2293, 2721, 2753
FaqI GGGAC 3 cut(s) 1033, 3645, 3660
FbaI TGATCA 4 cut(s) 75, 802, 1672, 3142
Fnu4HI GCNGC 7 cut(s) 686, 705, 1557, 1812, 2172, 2760, 3242
FriOI GRGCYC 1 cut(s) 2340
Fsp4HI GCNGC 7 cut(s) 686, 705, 1557, 1812, 2172, 2760, 3242
FspBI CTAG 9 cut(s) 537, 581, 1487, 1592, 1985, 2390, 2666, 2795, 3006
GlaI GCGC 1 cut(s) 2975
GluI GCNGC 7 cut(s) 686, 705, 1557, 1812, 2172, 2760, 3242
GsaI CCCAGC 1 cut(s) 3604
GsuI CTGGAG 2 cut(s) 2354, 3297
HaeIII GGCC 4 cut(s) 117, 2028, 2574, 3073
HhaI GCGC 1 cut(s) 2976
Hin6I GCGC 1 cut(s) 2974
HinP1I GCGC 1 cut(s) 2974
HincII GTYRAC 2 cut(s) 1204, 3610
HindII GTYRAC 2 cut(s) 1204, 3610
HindIII AAGCTT 1 cut(s) 2930
HpaI GTTAAC 2 cut(s) 1204, 3610
Hpy166II GTNNAC 8 cut(s) 13, 578, 606, 1204, 1448, 1946, 3233, 3610
Hpy8I GTNNAC 8 cut(s) 13, 578, 606, 1204, 1448, 1946, 3233, 3610
Hpy99I CGWCG 2 cut(s) 53, 1119
HpyAV CCTTC 8 cut(s) 433, 1170, 1519, 1814, 1840, 2310, 2955, 3840
HpyCH4III ACNGT 3 cut(s) 1915, 2091, 3043
HpyCH4IV ACGT 2 cut(s) 48, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 469, 732, 2025, 2938
HpySE526I ACGT 2 cut(s) 48, 533
HspAI GCGC 1 cut(s) 2974
KpnI GGTACC 1 cut(s) 3410
Ksp22I TGATCA 4 cut(s) 75, 802, 1672, 3142
KspAI GTTAAC 2 cut(s) 1204, 3610
LguI GCTCTTC 2 cut(s) 310, 1280
LmnI GCTCC 5 cut(s) 874, 1630, 2335, 3220, 3278
Lsp1109I GCAGC 7 cut(s) 691, 697, 1568, 1823, 2158, 2746, 3253
LweI GCATC 9 cut(s) 16, 2028, 2104, 2241, 2662, 2746, 2771, 3319, 3721
MaeI CTAG 9 cut(s) 537, 581, 1487, 1592, 1985, 2390, 2666, 2795, 3006
MaeII ACGT 2 cut(s) 48, 533
MfeI CAATTG 3 cut(s) 1389, 2576, 2860
MflI RGATCY 4 cut(s) 1759, 2830, 3131, 3491
MhlI GDGCHC 3 cut(s) 2340, 2706, 3870
MlsI TGGCCA 3 cut(s) 117, 2028, 2574
MluNI TGGCCA 3 cut(s) 117, 2028, 2574
MlyI GAGTC 9 cut(s) 318, 1481, 1616, 2141, 2515, 2940, 3102, 3455, 3682
MmeI TCCRAC 4 cut(s) 171, 1507, 2037, 2949
Mox20I TGGCCA 3 cut(s) 117, 2028, 2574
Mph1103I ATGCAT 3 cut(s) 898, 1513, 2309
MroXI GAANNNNTTC 3 cut(s) 1188, 1581, 1803
MscI TGGCCA 3 cut(s) 117, 2028, 2574
MslI CAYNNNNRTG 2 cut(s) 798, 3290
Msp20I TGGCCA 3 cut(s) 117, 2028, 2574
MspA1I CMGCKG 2 cut(s) 1559, 1814
MspCI CTTAAG 4 cut(s) 1604, 2258, 3103, 3236
MspR9I CCNGG 2 cut(s) 2025, 2333
MunI CAATTG 3 cut(s) 1389, 2576, 2860
Mva1269I GAATGC 2 cut(s) 41, 1348
MvaI CCWGG 2 cut(s) 2025, 2333
MwoI GCNNNNNNNGC 4 cut(s) 469, 732, 2025, 2938
NcoI CCATGG 1 cut(s) 1038
NlaIV GGNNCC 5 cut(s) 876, 1450, 1632, 3408, 3493
NmeAIII GCCGAG 1 cut(s) 3049
NmuCI GTSAC 4 cut(s) 539, 2144, 2867, 3700
NsiI ATGCAT 3 cut(s) 898, 1513, 2309
NspI RCATGY 4 cut(s) 1591, 2311, 3289, 3638
PaeI GCATGC 1 cut(s) 1591
PagI TCATGA 3 cut(s) 823, 1192, 3055
PciSI GCTCTTC 2 cut(s) 310, 1280
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 2 cut(s) 41, 1348
PdmI GAANNNNTTC 3 cut(s) 1188, 1581, 1803
PfeI GAWTC 8 cut(s) 343, 935, 1582, 2987, 3059, 3314, 3535, 3794
PflMI CCANNNNNTGG 1 cut(s) 276
PkrI GCNGC 7 cut(s) 687, 706, 1558, 1813, 2173, 2761, 3243
PleI GAGTC 9 cut(s) 317, 1480, 1616, 2141, 2515, 2940, 3101, 3454, 3681
PpsI GAGTC 9 cut(s) 317, 1480, 1616, 2141, 2515, 2940, 3101, 3454, 3681
PpuMI RGGWCCY 1 cut(s) 3304
PshBI ATTAAT 3 cut(s) 2544, 3365, 3824
PsiI TTATAA 1 cut(s) 1779
Psp124BI GAGCTC 1 cut(s) 2340
Psp5II RGGWCCY 1 cut(s) 3304
Psp6I CCWGG 2 cut(s) 2023, 2331
PspFI CCCAGC 1 cut(s) 3600
PspGI CCWGG 2 cut(s) 2023, 2331
PspN4I GGNNCC 5 cut(s) 876, 1450, 1632, 3408, 3493
PspPI GGNCC 3 cut(s) 1121, 1448, 3304
PspPPI RGGWCCY 1 cut(s) 3304
PstI CTGCAG 1 cut(s) 1558
PsuI RGATCY 4 cut(s) 1759, 2830, 3131, 3491
PvuII CAGCTG 2 cut(s) 1559, 1814
RsaI GTAC 5 cut(s) 416, 639, 1658, 2140, 3408
RsaNI GTAC 5 cut(s) 415, 638, 1657, 2139, 3407
RseI CAYNNNNRTG 2 cut(s) 798, 3290
SacI GAGCTC 1 cut(s) 2340
SapI GCTCTTC 2 cut(s) 310, 1280
SatI GCNGC 7 cut(s) 686, 705, 1557, 1812, 2172, 2760, 3242
Sau96I GGNCC 3 cut(s) 1121, 1448, 3304
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 9 cut(s) 318, 1481, 1616, 2141, 2515, 2940, 3102, 3455, 3682
ScrFI CCNGG 2 cut(s) 2025, 2333
SduI GDGCHC 3 cut(s) 2340, 2706, 3870
SfaNI GCATC 9 cut(s) 16, 2028, 2104, 2241, 2662, 2746, 2771, 3319, 3721
SfcI CTRYAG 3 cut(s) 930, 1097, 1554
SinI GGWCC 3 cut(s) 1121, 1448, 3304
SmiMI CAYNNNNRTG 2 cut(s) 798, 3290
SmlI CTYRAG 8 cut(s) 224, 950, 1604, 2258, 3103, 3219, 3236, 3476
SmoI CTYRAG 8 cut(s) 224, 950, 1604, 2258, 3103, 3219, 3236, 3476
SpeI ACTAGT 2 cut(s) 1486, 2794
SphI GCATGC 1 cut(s) 1591
SspI AATATT 1 cut(s) 2555
SspMI CTAG 9 cut(s) 537, 581, 1487, 1592, 1985, 2390, 2666, 2795, 3006
SstI GAGCTC 1 cut(s) 2340
StyD4I CCNGG 2 cut(s) 2023, 2331
StyI CCWWGG 5 cut(s) 112, 234, 369, 1038, 1626
TaaI ACNGT 3 cut(s) 1915, 2091, 3043
TaiI ACGT 2 cut(s) 51, 536
TaqI TCGA 8 cut(s) 51, 1117, 1475, 2187, 2632, 2706, 3533, 3870
TaqII GACCGA 1 cut(s) 1694
TatI WGTACW 3 cut(s) 414, 1656, 2138
TfiI GAWTC 8 cut(s) 343, 935, 1582, 2987, 3059, 3314, 3535, 3794
TscAI CASTG 6 cut(s) 1408, 1933, 2329, 3691, 3715, 3721
TseFI GTSAC 4 cut(s) 539, 2144, 2867, 3700
TseI GCWGC 7 cut(s) 685, 704, 1556, 1811, 2171, 2759, 3241
Tsp45I GTSAC 4 cut(s) 539, 2144, 2867, 3700
TspGWI ACGGA 1 cut(s) 3086
TspRI CASTG 6 cut(s) 1408, 1933, 2329, 3691, 3715, 3721
Van91I CCANNNNNTGG 1 cut(s) 276
Vha464I CTTAAG 4 cut(s) 1604, 2258, 3103, 3236
VpaK11BI GGWCC 3 cut(s) 1121, 1448, 3304
VspI ATTAAT 3 cut(s) 2544, 3365, 3824
XceI RCATGY 4 cut(s) 1591, 2311, 3289, 3638
XmnI GAANNNNTTC 3 cut(s) 1188, 1581, 1803
XspI CTAG 9 cut(s) 537, 581, 1487, 1592, 1985, 2390, 2666, 2795, 3006
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 3 cut(s) 898, 1513, 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.