Rw1G038170

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007218
Physical Location & Seq
Reverse (-)
65774956 .. 65783491
8536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G038170.1

Sequence Viewer

Length: 3585 bp
ATGCAAGTGGAATATTGTATCAAGGATTTGATACCTATTATTGAGCACTGCCAAGATGATCGTAGCTTGGTTTTAAATGCAGTGAAGGTTCTGGTGTTCCTGACTATGCCAGTGGAGCCTACATCAAATGACATTTTACAACAAATTGAATTTCTATGGGAGTTGAAGTCTTCAATTACGTCTAGTGACATTGTTGCTGTGATAGTTTCACTTCTGGAAAGTCCACTAGAAAATCTGGAAAGTGATGTGTTCACAGAGGATGACTGGAAATTGGTGCAGCTGGTACTTACTTTATTCCGAAACATTTTAGCCATTCAAGACATTTCATTGCAGCAGAAGTCTGGGGGTACTGCCAGTCAATTTCTATCACGCAGAGATAGCTTTCTGGAACTTTTGTTTCGTGAAAATGTGATGGACTTGGTTTTAGCCATAACACAGCATATTGGTGATCATCGTAGTTATCTCTCTCATGATAACTTGCTTCTATTGGAAATTTTCCATTATACATTTATAGGGCAAGAGCCAGAGTTGATTGCAAATGCATATTCAAAAGGTGCTAAGGTGGAAGAAGATACTATAGATTCTGTAAATAGTCTCAAGTCTATTATGGAGGAAGAGAAAGAGAAAAAGAGACTAAAAGTTAGTCGTCATTCCAATTTTGGCGGGACATTTATTCGGCTTACCATGGATGGTTCTAAGGAAGTTGTCAAGGGAAGACCTACTTCTGGTTCTTGTAATACCCTACAGAAATCACAAAATCGTCGAGGTCCAATAAAAAAGATTGCTTGGGATCATGGGATATTACCTTCAACAGAGGATAAGATTTTGGAATTGCTTCATGATTTTATTAACCAGTTTTTATCAGGGGGTTACAATGTTTTAATGCAGTCAATTCGTCAGGCTATTGAAAAGGAACACCACTCAATCCAGAAGAGTGATGTCATTATCTTCTTTCAGGTTGCTCAGTTTGTTACTTCTTTCCAATATCACAGGCATTCAATTTCTAAGCCAAGCCTGAGAGCTGAAGCAGACACAATTGAAGTTCCCGCAGGTAAAGAGGCTGATAGAACATTCTTTAGAGGTGATATATGTGGACCCATTGCAACCTCAATGAATGAGTCGATGTTCCAACTAGTCATTTCAAAGTGGCGTTTTGCATTCGATGGGTTGAAGGAAACACATGACTACAAGTTTCTGTCTGCAGCTGGTTCTCTTATGAAAGAAATGATTCGCATGCTAGATTTGGTGCTTAAGTTATTGCCAGAAGACTCCAAGGAGCCTCAAACTGCTCGCATACTTCTGTACAAGTTATTTTATGATCAGACCGAGGAGGGTATGACCCATTTTCTCTTAGGGTTGCTGAAATCATTTGACACTCACAAACAACCAAGAAGTGATCTTGCAGATCTGGTAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCATCTGGAACATTGAGGGTTTCTAAGAAGTCAAGGAAGGGGAGGAAGAGGAGAAAACTGAGTGAAAAGGAAACAGAAAATAAGCTCTCAGGAGAGCATGATATGGTTCAGAAAGAGACTGACATCTCCAATGGTGAACAATTAACAGATAAGAGTGTAACCGAGAAAAGAAGCCTAGACACAAGTTCTAATGAGAAACAAGACATCAGCATCCCTGGCCAACCTGATGAAGGCAAAAACTCTTTGTTGGAGACAGAAAACTGCTCGGGCAGTCAGGCACAGTTAGATCACAAAAATTCTGGTGATGCAAATGGTGATCTTTGTTATAGTACAGGTGACTCTTCTGGTGATGAGCAAGTAGCTGCAACTACTGAAGTCGATTTTAAGGTGTCAAGTTTGGTTTCTGCTTTTTCAAACAACAGCATCATCCAGAAATTGTGTTGGTTGCTTAAGTTCTATAAGAGTAACTTGACCAGAACAAATCACTACATAGTATGCATGTTGAGAAGGATCAGTGAAGACCTGGAGCTCTCTCCAATGCTGTATCAGTTATCACTCCTCACTATATTCTATGATATCCTAGCTGAGCAGAATTCATCTCCTTGCAACGAATATGAAAACATTGTTGGTTTCTTGAAAAGTTTGGTCAGGAAAATGCTAAAAAAAATGAAACATCAACCCTTACTTTTTGTGGAAATTCTCTTCTGGAAGACTCGCAAAGAGTGTCATTACATTAATGCTGAATATTTATTGCATGAGCTTGGCCAATTGAGGAATGAAAGTAGAAACTGGGGAGATACTTTAGAGGATGGAGATATGGGTCGATCGATGGACAAGGGATGGACAGCTAGAAGTCTAGCAGATGCACTTGGTGAAGATGAAGCTGATGTTGTGCTCGACTTTGGACATAACAACAATGAAGAAAATCTTGGAAAAGTGAAAGGTGATGCTGCATCTACTTCAGATAATGAGATTGATGAAAGCGGGGGCCAGTCCATGAAAACTGAAACTCGAAGGGGTCCTCTAAAAAAGAAAAGACTAGTTATTGGAGAGGAACAAGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGAGAACCACAATTGTAGTCACCTAATTGCCGAAGCTCTTGATCCTGATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAACAGCTTGGACTCAAAGTTGCACGAAGGAGAAGATTGCGCCATGGACAGGAATCTGTTTCTGCTGAGCCTAGTCAAACTGATGGAGCTGGAGAAGTAGAGGAAACAGTTATTACTCATCATGAATCAAAAACTCGGCCTTTGCACACAAGAAAAAGAGTCCGTGGCTTAAACGAAGAACAGGAAGCTCGAATTAGATCTCTCTATGATCAGTTTAAAGACCATAAAAGTTGCAACCACATGATTGCCAATGCAATGGATGGGGATGATAAATTGACTGCTCCTCAAGTTTCTCGTAAACTTAAGCAGCTTGGGTTATGTATTCCTAGGAAGAAGAGGTCTGGAGCTAACATGCCGATGAGGGATCAGGACCTAAATGATTCTGACACAAACGATGCCAATGCCTCTGATGATGAAACATTGTTATCGTTAATGAACAGGGGCAAAAAGGATAATATCAGAATTAGTGAGGTACCAGAACAGATTACCAGAAGAGAAGTGTCAGAAGAGGAGTCTGATGATGAAATGCTAAGCTCTGTTCTCAGGAAAGCTAAGGGATCCCTTTCTAAATTAAAGGATCAAAACCTCACAGCCATCTCGATTCAGGGAACAGCAAGAGGAAATGCTTTAGAAAATAAAGTTGAAGAAGCTGTTGTATCTTCTGGCAGGGATGTTAACGAGCAATCCTCTGGGTTGGGACATGCCGAAATTCACGATGTAGATACTTTCAATCATGCGAGTCCCAATGGCACTGCACAAGCTGAAGTCACAAGCACCGGCAGTGGTAATCTATTAGATGCTACACCTGTGAAAAATGTAGAGGATTTGGAACATCAGAAAATTGGCATTGACTTGGAAGATTCAGGGGATGAAGCAACTCATAGTGCATTAATACAGGGTGGTGCGAGTAGAAGGAAGATGAGGATGATGGTACTCGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1194

Amino Acids

134.68

Weight (kDa)

5.6

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 7 - 224 1.6e-44 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1425
Acc36I ACCTGC 1 cut(s) 1040
Acc65I GGTACC 1 cut(s) 3106
AccB1I GGYRCC 1 cut(s) 3106
AciI CCGC 3 cut(s) 663, 1047, 2439
AclWI GGATC 8 cut(s) 798, 1973, 2538, 2585, 3006, 3186, 3199, 3219
AcoI YGGCCR 2 cut(s) 1672, 2218
AcsI RAATTY 9 cut(s) 149, 492, 1750, 2047, 2151, 2543, 2604, 2619, 3342
AcuI CTGAAG 4 cut(s) 1044, 1848, 2400, 3417
AdeI CACNNNGTG 1 cut(s) 2327
AfaI GTAC 6 cut(s) 285, 349, 1304, 1786, 3108, 3567
AfiI CCNNNNNNNGG 3 cut(s) 725, 1685, 2683
AflII CTTAAG 3 cut(s) 1250, 1904, 2936
AhlI ACTAGT 2 cut(s) 1132, 2494
AjnI CCWGG 2 cut(s) 1669, 1977
AjuI GAANNNNNNNTTGG 4 cut(s) 2064, 2096, 2367, 2399
Alw21I GWGCWC 3 cut(s) 48, 1986, 2352
Alw26I GTCTC 4 cut(s) 599, 625, 1565, 1700
AlwI GGATC 8 cut(s) 798, 1973, 2538, 2585, 3006, 3186, 3199, 3219
Ama87I CYCGRG 1 cut(s) 1720
AoxI GGCC 4 cut(s) 1672, 2218, 2443, 2771
ApeKI GCWGC 6 cut(s) 277, 331, 1202, 1817, 2405, 2941
ApoI RAATTY 9 cut(s) 149, 492, 1750, 2047, 2151, 2543, 2604, 2619, 3342
AseI ATTAAT 2 cut(s) 2190, 3524
Asp700I GAANNNNTTC 3 cut(s) 834, 1227, 1449
Asp718I GGTACC 1 cut(s) 3106
AspA2I CCTAGG 1 cut(s) 2960
AspLEI GCGC 1 cut(s) 2676
AspS9I GGNCC 5 cut(s) 767, 1094, 2443, 2474, 3004
AvaI CYCGRG 1 cut(s) 1720
AvaII GGWCC 4 cut(s) 767, 1094, 2474, 3004
AvrII CCTAGG 1 cut(s) 2960
BalI TGGCCA 2 cut(s) 1674, 2220
BamHI GGATCC 1 cut(s) 3191
BanI GGYRCC 1 cut(s) 3106
BanII GRGCYC 1 cut(s) 1986
BbsI GAAGAC 5 cut(s) 162, 721, 1272, 1980, 2171
Bbv12I GWGCWC 3 cut(s) 48, 1986, 2352
BbvI GCAGC 6 cut(s) 289, 343, 1214, 1804, 2392, 2953
BceAI ACGGC 1 cut(s) 1451
BcgI CGANNNNNNTGC 4 cut(s) 875, 909, 3017, 3051
BciT130I CCWGG 2 cut(s) 1671, 1979
BclI TGATCA 3 cut(s) 448, 1318, 2842
BcoDI GTCTC 4 cut(s) 599, 625, 1565, 1700
BcuI ACTAGT 2 cut(s) 1132, 2494
BfmI CTRYAG 3 cut(s) 576, 743, 1200
BfrI CTTAAG 3 cut(s) 1250, 1904, 2936
BfuAI ACCTGC 1 cut(s) 1040
BglII AGATCT 2 cut(s) 1405, 2831
BisI GCNGC 6 cut(s) 278, 332, 1203, 1818, 2406, 2942
BlnI CCTAGG 1 cut(s) 2960
BlpI GCTNAGC 3 cut(s) 2040, 2700, 3164
BlsI GCNGC 6 cut(s) 279, 333, 1204, 1819, 2407, 2943
Bme1390I CCNGG 2 cut(s) 1671, 1979
Bme18I GGWCC 4 cut(s) 767, 1094, 2474, 3004
BmeT110I CYCGRG 1 cut(s) 1720
BmgT120I GGNCC 5 cut(s) 767, 1094, 2443, 2474, 3004
BmiI GGNNCC 7 cut(s) 117, 1096, 1278, 2444, 2475, 3108, 3193
BmrFI CCNGG 2 cut(s) 1671, 1979
BmrI ACTGGG 1 cut(s) 2254
BmsI GCATC 9 cut(s) 1466, 1674, 1750, 1887, 2308, 2392, 2417, 3019, 3421
BmuI ACTGGG 1 cut(s) 2254
BpiI GAAGAC 5 cut(s) 162, 721, 1272, 1980, 2171
BplI GAGNNNNNCTC 2 cut(s) 3305, 3337
BpmI CTGGAG 3 cut(s) 2000, 2745, 2997
Bpu10I CCTNAGC 2 cut(s) 558, 3186
Bpu1102I GCTNAGC 3 cut(s) 2040, 2700, 3164
BpuEI CTTGAG 2 cut(s) 581, 2904
Bsa29I ATCGAT 1 cut(s) 2282
BsaJI CCNNGG 7 cut(s) 684, 1272, 1326, 1669, 2677, 2797, 2960
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 3 cut(s) 725, 1685, 2683
Bse118I RCCGGY 1 cut(s) 3410
Bse1I ACTGG 6 cut(s) 110, 269, 354, 853, 2249, 2446
Bse3DI GCAATG 3 cut(s) 326, 1098, 2895
BseBI CCWGG 2 cut(s) 1671, 1979
BseCI ATCGAT 1 cut(s) 2282
BseDI CCNNGG 7 cut(s) 684, 1272, 1326, 1669, 2677, 2797, 2960
BseLI CCNNNNNNNGG 3 cut(s) 725, 1685, 2683
BseMI GCAATG 3 cut(s) 326, 1098, 2895
BseMII CTCAG 7 cut(s) 977, 1007, 1505, 1557, 2031, 2691, 3190
BseNI ACTGG 6 cut(s) 110, 269, 354, 853, 2249, 2446
BseRI GAGGAG 5 cut(s) 1343, 1519, 2003, 2907, 3158
BseXI GCAGC 6 cut(s) 289, 343, 1214, 1804, 2392, 2953
BsgI GTGCAG 2 cut(s) 296, 3372
Bsh1285I CGRYCG 1 cut(s) 2282
BshFI GGCC 4 cut(s) 1674, 2220, 2445, 2773
BshNI GGYRCC 1 cut(s) 3106
BshVI ATCGAT 1 cut(s) 2282
BsiEI CGRYCG 1 cut(s) 2282
BsiHKAI GWGCWC 3 cut(s) 48, 1986, 2352
BsiHKCI CYCGRG 1 cut(s) 1720
BsiSI CCGG 1 cut(s) 3411
BslFI GGGAC 3 cut(s) 679, 3345, 3360
BslI CCNNNNNNNGG 3 cut(s) 725, 1685, 2683
BsmAI GTCTC 4 cut(s) 599, 625, 1565, 1700
BsmFI GGGAC 3 cut(s) 679, 3345, 3360
BsmI GAATGC 2 cut(s) 994, 1157
BsnI GGCC 4 cut(s) 1674, 2220, 2445, 2773
BsoBI CYCGRG 1 cut(s) 1720
Bsp1286I GDGCHC 3 cut(s) 48, 1986, 2352
Bsp1407I TGTACA 1 cut(s) 1302
Bsp1720I GCTNAGC 3 cut(s) 2040, 2700, 3164
Bsp19I CCATGG 2 cut(s) 684, 2677
BspACI CCGC 3 cut(s) 663, 1047, 2439
BspANI GGCC 4 cut(s) 1674, 2220, 2445, 2773
BspCNI CTCAG 7 cut(s) 976, 1008, 1506, 1556, 2032, 2692, 3189
BspDI ATCGAT 1 cut(s) 2282
BspHI TCATGA 3 cut(s) 469, 838, 2755
BspLI GGNNCC 7 cut(s) 117, 1096, 1278, 2444, 2475, 3108, 3193
BspMAI CTGCAG 1 cut(s) 1204
BspMI ACCTGC 1 cut(s) 1040
BspPI GGATC 8 cut(s) 798, 1973, 2538, 2585, 3006, 3186, 3199, 3219
BspT107I GGYRCC 1 cut(s) 3106
BspTI CTTAAG 3 cut(s) 1250, 1904, 2936
BsrDI GCAATG 3 cut(s) 326, 1098, 2895
BsrFI RCCGGY 1 cut(s) 3410
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 6 cut(s) 110, 269, 354, 853, 2249, 2446
BssAI RCCGGY 1 cut(s) 3410
BssECI CCNNGG 7 cut(s) 684, 1272, 1326, 1669, 2677, 2797, 2960
BssT1I CCWWGG 4 cut(s) 684, 1272, 2677, 2960
Bst2UI CCWGG 2 cut(s) 1671, 1979
Bst4CI ACNGT 2 cut(s) 1737, 2743
Bst6I CTCTTC 8 cut(s) 609, 926, 1496, 1801, 2162, 2963, 3121, 3135
BstAFI CTTAAG 3 cut(s) 1250, 1904, 2936
BstAUI TGTACA 1 cut(s) 1302
BstC8I GCNNGC 2 cut(s) 1235, 1291
BstDSI CCRYGG 3 cut(s) 684, 2677, 2797
BstENI CCTNNNNNAGG 1 cut(s) 1683
BstHHI GCGC 1 cut(s) 2676
BstMAI GTCTC 4 cut(s) 599, 625, 1565, 1700
BstMCI CGRYCG 1 cut(s) 2282
BstMWI GCNNNNNNNGC 4 cut(s) 115, 378, 1671, 2638
BstNI CCWGG 2 cut(s) 1671, 1979
BstNSI RCATGY 4 cut(s) 1237, 1957, 2989, 3338
BstSCI CCNGG 2 cut(s) 1669, 1977
BstSFI CTRYAG 3 cut(s) 576, 743, 1200
BstV1I GCAGC 6 cut(s) 289, 343, 1214, 1804, 2392, 2953
BstV2I GAAGAC 5 cut(s) 162, 721, 1272, 1980, 2171
BstX2I RGATCY 4 cut(s) 1405, 2530, 2831, 3191
BstXI CCANNNNNNTGG 1 cut(s) 2890
BstYI RGATCY 4 cut(s) 1405, 2530, 2831, 3191
Bsu15I ATCGAT 1 cut(s) 2282
BsuRI GGCC 4 cut(s) 1674, 2220, 2445, 2773
BsuTUI ATCGAT 1 cut(s) 2282
BtgI CCRYGG 3 cut(s) 684, 2677, 2797
BtsI GCAGTG 4 cut(s) 46, 87, 3384, 3421
BtsIMutI CAGTG 6 cut(s) 46, 87, 117, 1975, 3384, 3421
BveI ACCTGC 1 cut(s) 1040
Cac8I GCNNGC 2 cut(s) 1235, 1291
CciI TCATGA 3 cut(s) 469, 838, 2755
CfoI GCGC 1 cut(s) 2676
Cfr10I RCCGGY 1 cut(s) 3410
Cfr13I GGNCC 5 cut(s) 767, 1094, 2443, 2474, 3004
ClaI ATCGAT 1 cut(s) 2282
Csp6I GTAC 6 cut(s) 284, 348, 1303, 1785, 3107, 3566
CviQI GTAC 6 cut(s) 284, 348, 1303, 1785, 3107, 3566
DraI TTTAAA 2 cut(s) 75, 2851
DraIII CACNNNGTG 1 cut(s) 2327
EaeI YGGCCR 2 cut(s) 1672, 2218
Eam1104I CTCTTC 8 cut(s) 609, 926, 1496, 1801, 2162, 2963, 3121, 3135
EarI CTCTTC 8 cut(s) 609, 926, 1496, 1801, 2162, 2963, 3121, 3135
Ecl136II GAGCTC 1 cut(s) 1984
Eco130I CCWWGG 4 cut(s) 684, 1272, 2677, 2960
Eco24I GRGCYC 1 cut(s) 1986
Eco32I GATATC 1 cut(s) 2032
Eco47I GGWCC 4 cut(s) 767, 1094, 2474, 3004
Eco53kI GAGCTC 1 cut(s) 1984
Eco57I CTGAAG 4 cut(s) 1044, 1848, 2400, 3417
Eco88I CYCGRG 1 cut(s) 1720
EcoICRI GAGCTC 1 cut(s) 1984
EcoNI CCTNNNNNAGG 1 cut(s) 1683
EcoO109I RGGNCCY 2 cut(s) 2474, 3004
EcoRI GAATTC 1 cut(s) 2047
EcoRII CCWGG 2 cut(s) 1669, 1977
EcoRV GATATC 1 cut(s) 2032
EcoT14I CCWWGG 4 cut(s) 684, 1272, 2677, 2960
EcoT22I ATGCAT 2 cut(s) 544, 1955
EcoT38I GRGCYC 1 cut(s) 1986
ErhI CCWWGG 4 cut(s) 684, 1272, 2677, 2960
FalI AAGNNNNNCTT 6 cut(s) 706, 738, 1907, 1939, 2367, 2399
FaqI GGGAC 3 cut(s) 679, 3345, 3360
FauI CCCGC 3 cut(s) 656, 1054, 2432
FbaI TGATCA 3 cut(s) 448, 1318, 2842
Fnu4HI GCNGC 6 cut(s) 278, 332, 1203, 1818, 2406, 2942
FriOI GRGCYC 1 cut(s) 1986
Fsp4HI GCNGC 6 cut(s) 278, 332, 1203, 1818, 2406, 2942
GlaI GCGC 1 cut(s) 2675
GluI GCNGC 6 cut(s) 278, 332, 1203, 1818, 2406, 2942
GsuI CTGGAG 3 cut(s) 2000, 2745, 2997
HaeIII GGCC 4 cut(s) 1674, 2220, 2445, 2773
HapII CCGG 1 cut(s) 3411
HhaI GCGC 1 cut(s) 2676
Hin6I GCGC 1 cut(s) 2674
HinP1I GCGC 1 cut(s) 2674
HincII GTYRAC 1 cut(s) 3310
HindII GTYRAC 1 cut(s) 3310
HindIII AAGCTT 1 cut(s) 2630
HpaI GTTAAC 1 cut(s) 3310
HpaII CCGG 1 cut(s) 3411
Hpy166II GTNNAC 6 cut(s) 224, 252, 1094, 1592, 2933, 3310
Hpy8I GTNNAC 6 cut(s) 224, 252, 1094, 1592, 2933, 3310
Hpy99I CGWCG 1 cut(s) 765
HpyCH4III ACNGT 2 cut(s) 1737, 2743
HpyCH4IV ACGT 1 cut(s) 179
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 378, 1671, 2638
HpySE526I ACGT 1 cut(s) 179
HspAI GCGC 1 cut(s) 2674
KpnI GGTACC 1 cut(s) 3110
Ksp22I TGATCA 3 cut(s) 448, 1318, 2842
KspAI GTTAAC 1 cut(s) 3310
LmnI GCTCC 6 cut(s) 115, 1276, 1981, 2720, 2920, 2978
Lsp1109I GCAGC 6 cut(s) 289, 343, 1214, 1804, 2392, 2953
LweI GCATC 9 cut(s) 1466, 1674, 1750, 1887, 2308, 2392, 2417, 3019, 3421
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 8 cut(s) 185, 869, 970, 1612, 1790, 1919, 2567, 3400
MfeI CAATTG 3 cut(s) 1035, 2222, 2560
MflI RGATCY 4 cut(s) 1405, 2530, 2831, 3191
MhlI GDGCHC 3 cut(s) 48, 1986, 2352
MlsI TGGCCA 2 cut(s) 1674, 2220
MluNI TGGCCA 2 cut(s) 1674, 2220
MlyI GAGTC 8 cut(s) 1127, 1262, 1787, 2161, 2640, 2802, 3155, 3382
MmeI TCCRAC 3 cut(s) 1153, 1683, 2649
Mox20I TGGCCA 2 cut(s) 1674, 2220
Mph1103I ATGCAT 2 cut(s) 544, 1955
MroXI GAANNNNTTC 3 cut(s) 834, 1227, 1449
MscI TGGCCA 2 cut(s) 1674, 2220
MslI CAYNNNNRTG 2 cut(s) 444, 2990
Msp20I TGGCCA 2 cut(s) 1674, 2220
MspA1I CMGCKG 2 cut(s) 280, 1205
MspCI CTTAAG 3 cut(s) 1250, 1904, 2936
MspI CCGG 1 cut(s) 3411
MspR9I CCNGG 2 cut(s) 1671, 1979
MunI CAATTG 3 cut(s) 1035, 2222, 2560
Mva1269I GAATGC 2 cut(s) 994, 1157
MvaI CCWGG 2 cut(s) 1671, 1979
MwoI GCNNNNNNNGC 4 cut(s) 115, 378, 1671, 2638
NcoI CCATGG 2 cut(s) 684, 2677
NlaIV GGNNCC 7 cut(s) 117, 1096, 1278, 2444, 2475, 3108, 3193
NmeAIII GCCGAG 1 cut(s) 2749
NmuCI GTSAC 4 cut(s) 185, 1790, 2567, 3400
NsiI ATGCAT 2 cut(s) 544, 1955
NspI RCATGY 4 cut(s) 1237, 1957, 2989, 3338
PaeI GCATGC 1 cut(s) 1237
PagI TCATGA 3 cut(s) 469, 838, 2755
PctI GAATGC 2 cut(s) 994, 1157
PdmI GAANNNNTTC 3 cut(s) 834, 1227, 1449
PfeI GAWTC 7 cut(s) 581, 1228, 2687, 2759, 3014, 3235, 3494
PkrI GCNGC 6 cut(s) 279, 333, 1204, 1819, 2407, 2943
Ple19I CGATCG 1 cut(s) 2282
PleI GAGTC 8 cut(s) 1126, 1262, 1787, 2161, 2640, 2801, 3154, 3381
PpsI GAGTC 8 cut(s) 1126, 1262, 1787, 2161, 2640, 2801, 3154, 3381
PpuMI RGGWCCY 2 cut(s) 2474, 3004
PshBI ATTAAT 2 cut(s) 2190, 3524
PsiI TTATAA 1 cut(s) 1425
Psp124BI GAGCTC 1 cut(s) 1986
Psp5II RGGWCCY 2 cut(s) 2474, 3004
Psp6I CCWGG 2 cut(s) 1669, 1977
PspGI CCWGG 2 cut(s) 1669, 1977
PspN4I GGNNCC 7 cut(s) 117, 1096, 1278, 2444, 2475, 3108, 3193
PspPI GGNCC 5 cut(s) 767, 1094, 2443, 2474, 3004
PspPPI RGGWCCY 2 cut(s) 2474, 3004
PstI CTGCAG 1 cut(s) 1204
PsuI RGATCY 4 cut(s) 1405, 2530, 2831, 3191
PvuI CGATCG 1 cut(s) 2282
PvuII CAGCTG 2 cut(s) 280, 1205
RsaI GTAC 6 cut(s) 285, 349, 1304, 1786, 3108, 3567
RsaNI GTAC 6 cut(s) 284, 348, 1303, 1785, 3107, 3566
RseI CAYNNNNRTG 2 cut(s) 444, 2990
SacI GAGCTC 1 cut(s) 1986
SatI GCNGC 6 cut(s) 278, 332, 1203, 1818, 2406, 2942
Sau96I GGNCC 5 cut(s) 767, 1094, 2443, 2474, 3004
SchI GAGTC 8 cut(s) 1127, 1262, 1787, 2161, 2640, 2802, 3155, 3382
ScrFI CCNGG 2 cut(s) 1671, 1979
SduI GDGCHC 3 cut(s) 48, 1986, 2352
SfaNI GCATC 9 cut(s) 1466, 1674, 1750, 1887, 2308, 2392, 2417, 3019, 3421
SfcI CTRYAG 3 cut(s) 576, 743, 1200
SinI GGWCC 4 cut(s) 767, 1094, 2474, 3004
SmiMI CAYNNNNRTG 2 cut(s) 444, 2990
SmlI CTYRAG 5 cut(s) 596, 1250, 1904, 2919, 2936
SmoI CTYRAG 5 cut(s) 596, 1250, 1904, 2919, 2936
SpeI ACTAGT 2 cut(s) 1132, 2494
SphI GCATGC 1 cut(s) 1237
SsiI CCGC 3 cut(s) 663, 1047, 2439
SspI AATATT 2 cut(s) 14, 2201
SstI GAGCTC 1 cut(s) 1986
StyD4I CCNGG 2 cut(s) 1669, 1977
StyI CCWWGG 4 cut(s) 684, 1272, 2677, 2960
TaaI ACNGT 2 cut(s) 1737, 2743
TaiI ACGT 1 cut(s) 182
TaqII GACCGA 1 cut(s) 1340
TatI WGTACW 2 cut(s) 1302, 1784
TfiI GAWTC 7 cut(s) 581, 1228, 2687, 2759, 3014, 3235, 3494
TscAI CASTG 6 cut(s) 53, 87, 117, 1975, 3391, 3421
TseFI GTSAC 4 cut(s) 185, 1790, 2567, 3400
TseI GCWGC 6 cut(s) 277, 331, 1202, 1817, 2405, 2941
Tsp45I GTSAC 4 cut(s) 185, 1790, 2567, 3400
TspGWI ACGGA 1 cut(s) 2786
TspRI CASTG 6 cut(s) 53, 87, 117, 1975, 3391, 3421
Vha464I CTTAAG 3 cut(s) 1250, 1904, 2936
VpaK11BI GGWCC 4 cut(s) 767, 1094, 2474, 3004
VspI ATTAAT 2 cut(s) 2190, 3524
XagI CCTNNNNNAGG 1 cut(s) 1683
XapI RAATTY 9 cut(s) 149, 492, 1750, 2047, 2151, 2543, 2604, 2619, 3342
XceI RCATGY 4 cut(s) 1237, 1957, 2989, 3338
XmaJI CCTAGG 1 cut(s) 2960
XmnI GAANNNNTTC 3 cut(s) 834, 1227, 1449
Zsp2I ATGCAT 2 cut(s) 544, 1955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.