RLG00000026367

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007218
Physical Location & Seq
Forward (+)
2432567 .. 2441110
8544 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026367

Sequence Viewer

Length: 3765 bp
ATGGATTTGCAAGGCTTATCGGTGATCTGCAACGGCCTCGGAATCGCCCTGGACGACGACGACGGAAACTTGATCGGTTACAGGAAGCACAAGTACTGCTTAGATAATTTGAAGGATTTGCTGAGATTCTTGAGGCGCGATGATCCACAGAACCGGGATGTTTTCAAGGAAGTTTGCAAGTGGAATATTGTATCTAAGGATTTGATACCTATTATTGAGCACTGCCAAGAGGATCGTAGCTTGGTTTTAAATGCAGTGAAGGTTCTGGTGTTCCTGACTATGCCAGTTGAGCCTACATCAAATGACATTTTACAACAAATTGAATTTCTATGGGAGTTGAAGTCTTCAATTACGTCTAGTGACATTGTTGCTGTTATAGTTTCACTTCTGGAAAGTCCACTAGAAAATCTGGAAAGTGATGTGTTCACAGAGGACGACTGGAAATTGGTGCAGCTGGTACTTTCTTTATTCCGAAACATTTTAGCCATTCAAGACATTTCACTGCAGCAGAAGTCTGGGGGTACTGCCAGTCAATTTCTATCACGCAGAGATAGCTTTCTGGAACTTTTGTTTCGTGAAAATGTGATGGACTTGGTTTTAGCCATAACACAGCATATTGGTGATCATCGTAGTTATCTCTCTCATGATAAGTTGCTTCTATTGGAAATTTTCCATTATACATTTATAGGGCAAGAGCCAGAGTTGATTGCAAATGCATATTCAAAAGGTGCTAAGGTGGAAGAAGATACTATAGATTCTGTAAATAGTCTCAAGTCTATTATGGAGGAAGAGAAGGAGAAAAAGAGACTAAAAGTTAGTCGTCATTCCAATTTTGGCGGGACATTTATTCGGCTTACCATGGATGGTTCTAAGGAAGTTGTCAAGGGAAGACCTACTTCTGCTTCTTGTAATACCCTACAGAAATCACAAAATCGTCGAGGTCCAATAAAAAAGATTGCTTGGGATCATGGGATATTACCTTCAACAGAGGATAAGATTTTGGAATTGCTTCATGATTTTGTTAACCAGTTTTTATCAGGGGGTTACAATGTTTTAATGCAGTCAATTCGTCAGGCTATTGAAAAGGAACACCACTCAATCCAGAAGAGTGATGTCATTATCTTCTTTCAGGTTGCTCAGTTTGTTACTTCTTTCCAATATCACAGGCATTCAATTTCTAAGCCAAGCCTGAGAGCTGAAGCAGACACAATTGAAGTTCCCGCCGGTAAAGAGGCTGACAGAACACTCTTTAGAGGTGATATATGTGGACCCATTGCAACCTCAATGAATGAGTCGATGTTCCAACTAGTCATTTCAAAGTGGCGTTATGCATTCGATGGGTTGAAGGAAACACATGACTACAAGTTTCTGTCTGCAGCTGGTTCTCTTATGAAAGAAATGATTCGCATGCTAGATTTGGTGCTTAAGTTATTGCCAGAAGACTCCAAGGAGCCTCAAACAGCTCGCATACTTCTGTACAAGTTATTTTATGATCAGACCGAGGAGGGGATGACCCATTTTCTCTTAGGGTTGCTGAAATCGTTTGACACTCACAAACAACCAAGAAGTGATCTTGCAGATCTGGTAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCATCTGGAACATTGAGGGTTTCTAAGAAGTCAAGGAAGGGGAGGAAGAGGAGAAAACTGAGTGAAAAGGGAACAGAAAATAAGCTCTCAGGAAAGCATGATATGGTTCAGAAAGAGACTGACATCTCCAATGGTGAACAATTAACAGATAAGAGTGTAACCGAGAAAAGAAGCCTAGACACAAGTTCCAATGAGAAACAAGACATCAGCATCCCTGGCCAACCTGATGAAGGCAAAAACTCTTTGTTGGAGACAGAAAACTTCTCGGGCAGTCAGGCACAGTTAGATCACAAAAATTCTGGTGATGCAAATGGTGATCTTTGTTATAGTACAGGTGACTCTTCTGGTGATGAGCAAGTAGCTGCAACTACTGAAGTCGATTTTAAGGTTTCAAGTTTCGTTTCTGCTTTTTCAAACAACAGCATCATCCATAAATTGTGTTGGTTGCTTAAGTTCTATAAGAATAACTTGACCAGAACAAACCACTACATAGTATGCATGTTGAGAAGGATCAGTGAAGACCTGGAGCTCTCTCCAATGCTGTATCAGTTATCACTCCTCACTATATTCTATGATATCCTAGCTGAGCAGAATTCATCTCCTTGCAATGAATATGACAACATTGTTGGTTTCTTGAAAAGTTTGGTCAGGAAAATGCTAAAAAAAATGAAACATCAACCCTTACTTTTTGTGGAAATTCTCTTCTGGAAGACTCGCAGAGAGTGTCATTACATTAATGCTGAATATTTATTGCATGAGCTTGGCCAACTGAGGAATGAAAGTAGAAACTGGGGAGATACTTTAGAGGATGGAGATATGGGTCGATCAATGGACAAGGGATGGACAGCTAGAAGTCTAGCAGATGCACTTGGTGAAGATGAAGCTGATGTTGTGCTCGACTTTGGACATAACATCAATGAAGAAAATCTTGGAAAAGTGAAAGGTGATGCTGCATCTACTTCAGATAATGAGATTGATGAAAGCGGGGGCCAGTCCATGAAAACTGAAACTCGAAGGGGTCCTCTAAAAAAGAAAAGACTAGTTATTGGAGAGGAACAGGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGAGAACCACAATTGTAGTCACCTAATTGCTGAAGCTCTTGATCCTGATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAACAGCTTGGACTCAAAGTTGCACGAAGGAGAAGATTGCGCCATGCACAGGAATCTGTTTCTGCTGAGCCTAGTCAAACTGATGGAGATGGAGAAGTAGAGGAAACAGTTATTACTCATCATGAATCAAAAACTCGGCCTTTGCACACAAGAAAAAGAGTCCGTGGCTTAAGCGAAGAACAGGAAGCTCAAATTAGATCTCTCTATGATCAGTTTAAAGACCATAAAAGTTGCAACCACATGATTGCCAATGCAATGGATGGGGATGATAAATTGACAGCTCCTCAAGTTTCTCGTAAACTTAAGCAGCTTGGGTTATGTATTCCTCGGAAGAAGAGGTCTGGAGCTAACATGCCGATGAGGGATCAGGACCTGAATGATTCTGACACAAACAATGCCAATGCCTCTGATGATGAAACATTGTTATCATTAATGAACAGGGGCAAAAAGGATAATATCAGAATTAGTGAGGTGCCAGAACAGATTACCAGAAGAGAAGTGTCAGAAGAGGAGTCTGATGATGAAATGCTAAGCTCTGTTCTCAAGAAAGCTAAGGGATCCCTTTCTAAATTAAAGGATCAAAACCTCACAGCCATCTCGATTCAGGGAACAGCAAGAGGAAATGCTTTAGAAATTAAGGTTGAAGAAGCTGTTGTATCTTCTGGCAGGGATGTTAACGAGCAATCCTCTGGGTTGGGACATGCCGAAATTCACGATGTAGATACTTTCAATCATGCGAGTCCCAATGGCACTGCACAAGCTGAAGTCACAAGCACCGGCAGTGGTAATCTATTAGATGCTACACCTGTGAAAATTGTAGAGGATTTGGAACATCAGAAAATCGGCATTGACTTGGAAGATTCAGGGGATGAAGCAACTCATGGTGCATTAATACAGGGTGGTGCAAGTAGAAGGAAGATGAGGATGATGGTACTCGAAGATGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1255

Amino Acids

141.57

Weight (kDa)

5.67

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 27 - 282 1.4e-59 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1599
AccB1I GGYRCC 1 cut(s) 3280
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 837, 1221, 2613
AcoI YGGCCR 2 cut(s) 1846, 2392
AcsI RAATTY 9 cut(s) 323, 666, 1924, 2221, 2325, 2717, 2778, 2793, 3516
AcuI CTGAAG 5 cut(s) 1218, 2022, 2574, 2775, 3591
AdeI CACNNNGTG 1 cut(s) 2501
AfaI GTAC 6 cut(s) 95, 459, 523, 1478, 1960, 3741
AfiI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
AflII CTTAAG 4 cut(s) 1424, 2078, 2977, 3110
AhlI ACTAGT 2 cut(s) 1306, 2668
AjnI CCWGG 3 cut(s) 48, 1843, 2151
AjuI GAANNNNNNNTTGG 2 cut(s) 2541, 2573
Alw21I GWGCWC 3 cut(s) 222, 2160, 2526
Alw26I GTCTC 4 cut(s) 773, 799, 1739, 1874
AlwNI CAGNNNCTG 1 cut(s) 3181
Ama87I CYCGRG 1 cut(s) 1894
AoxI GGCC 5 cut(s) 34, 1846, 2392, 2617, 2945
ApeKI GCWGC 6 cut(s) 451, 505, 1376, 1991, 2579, 3115
ApoI RAATTY 9 cut(s) 323, 666, 1924, 2221, 2325, 2717, 2778, 2793, 3516
AseI ATTAAT 3 cut(s) 2364, 3239, 3698
Asp700I GAANNNNTTC 3 cut(s) 1008, 1401, 1623
AspLEI GCGC 2 cut(s) 138, 2850
AspS9I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
AsuC2I CCSGG 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 1894
AvaII GGWCC 4 cut(s) 941, 1268, 2648, 3178
BalI TGGCCA 2 cut(s) 1848, 2394
BamHI GGATCC 1 cut(s) 3365
BanI GGYRCC 1 cut(s) 3280
BanII GRGCYC 1 cut(s) 2160
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 5 cut(s) 336, 895, 1446, 2154, 2345
Bbv12I GWGCWC 3 cut(s) 222, 2160, 2526
BbvI GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
BceAI ACGGC 2 cut(s) 49, 1625
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 1049, 1083
BciT130I CCWGG 3 cut(s) 50, 1845, 2153
BclI TGATCA 3 cut(s) 622, 1492, 3016
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 4 cut(s) 773, 799, 1739, 1874
BcuI ACTAGT 2 cut(s) 1306, 2668
BfmI CTRYAG 4 cut(s) 503, 750, 917, 1374
BfrI CTTAAG 4 cut(s) 1424, 2078, 2977, 3110
BglII AGATCT 2 cut(s) 1579, 3005
BisI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
BlpI GCTNAGC 3 cut(s) 2214, 2874, 3338
BlsI GCNGC 6 cut(s) 453, 507, 1378, 1993, 2581, 3117
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 4 cut(s) 50, 155, 1845, 2153
Bme18I GGWCC 4 cut(s) 941, 1268, 2648, 3178
BmeT110I CYCGRG 1 cut(s) 1894
BmgT120I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
BmiI GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
BmrFI CCNGG 4 cut(s) 50, 155, 1845, 2153
BmrI ACTGGG 1 cut(s) 2428
BmsI GCATC 8 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591, 3595
BmuI ACTGGG 1 cut(s) 2428
BpiI GAAGAC 5 cut(s) 336, 895, 1446, 2154, 2345
BplI GAGNNNNNCTC 2 cut(s) 3479, 3511
BpmI CTGGAG 2 cut(s) 2174, 3171
Bpu10I CCTNAGC 2 cut(s) 732, 3360
Bpu1102I GCTNAGC 3 cut(s) 2214, 2874, 3338
BpuEI CTTGAG 4 cut(s) 151, 755, 3078, 3335
BpuMI CCSGG 1 cut(s) 155
BsaJI CCNNGG 8 cut(s) 37, 48, 858, 1446, 1500, 1843, 2971, 3134
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
Bse118I RCCGGY 2 cut(s) 1223, 3584
Bse1I ACTGG 6 cut(s) 284, 443, 528, 1027, 2423, 2620
Bse3DI GCAATG 3 cut(s) 1272, 2242, 3069
BseBI CCWGG 3 cut(s) 50, 1845, 2153
BseDI CCNNGG 8 cut(s) 37, 48, 858, 1446, 1500, 1843, 2971, 3134
BseLI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
BseMI GCAATG 3 cut(s) 1272, 2242, 3069
BseMII CTCAG 8 cut(s) 113, 1151, 1181, 1679, 1731, 2205, 2390, 2865
BseNI ACTGG 6 cut(s) 284, 443, 528, 1027, 2423, 2620
BseRI GAGGAG 5 cut(s) 1517, 1693, 2177, 3081, 3332
BseXI GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
BsgI GTGCAG 2 cut(s) 470, 3546
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BshNI GGYRCC 1 cut(s) 3280
BsiHKAI GWGCWC 3 cut(s) 222, 2160, 2526
BsiHKCI CYCGRG 1 cut(s) 1894
BsiSI CCGG 3 cut(s) 154, 1224, 3585
BslFI GGGAC 3 cut(s) 853, 3519, 3534
BslI CCNNNNNNNGG 3 cut(s) 1506, 1859, 2857
BsmAI GTCTC 4 cut(s) 773, 799, 1739, 1874
BsmFI GGGAC 3 cut(s) 853, 3519, 3534
BsmI GAATGC 2 cut(s) 1168, 1331
BsnI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BsoBI CYCGRG 1 cut(s) 1894
Bsp1286I GDGCHC 3 cut(s) 222, 2160, 2526
Bsp1407I TGTACA 1 cut(s) 1476
Bsp1720I GCTNAGC 3 cut(s) 2214, 2874, 3338
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 3 cut(s) 837, 1221, 2613
BspANI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BspCNI CTCAG 8 cut(s) 114, 1150, 1182, 1680, 1730, 2206, 2391, 2866
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 3 cut(s) 643, 1012, 2929
BspLI GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
BspMAI CTGCAG 2 cut(s) 507, 1378
BspT107I GGYRCC 1 cut(s) 3280
BspTI CTTAAG 4 cut(s) 1424, 2078, 2977, 3110
BsrDI GCAATG 3 cut(s) 1272, 2242, 3069
BsrFI RCCGGY 2 cut(s) 1223, 3584
BsrGI TGTACA 1 cut(s) 1476
BsrI ACTGG 6 cut(s) 284, 443, 528, 1027, 2423, 2620
BssAI RCCGGY 2 cut(s) 1223, 3584
BssECI CCNNGG 8 cut(s) 37, 48, 858, 1446, 1500, 1843, 2971, 3134
BssT1I CCWWGG 2 cut(s) 858, 1446
Bst2UI CCWGG 3 cut(s) 50, 1845, 2153
Bst4CI ACNGT 2 cut(s) 1911, 2917
Bst6I CTCTTC 8 cut(s) 783, 1100, 1670, 1975, 2336, 3137, 3295, 3309
BstAFI CTTAAG 4 cut(s) 1424, 2078, 2977, 3110
BstAUI TGTACA 1 cut(s) 1476
BstC8I GCNNGC 2 cut(s) 1409, 1465
BstDSI CCRYGG 2 cut(s) 858, 2971
BstENI CCTNNNNNAGG 1 cut(s) 1857
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 2 cut(s) 138, 2850
BstMAI GTCTC 4 cut(s) 773, 799, 1739, 1874
BstMWI GCNNNNNNNGC 4 cut(s) 289, 552, 1845, 2812
BstNI CCWGG 3 cut(s) 50, 1845, 2153
BstNSI RCATGY 4 cut(s) 1411, 2131, 3163, 3512
BstSCI CCNGG 4 cut(s) 48, 153, 1843, 2151
BstSFI CTRYAG 4 cut(s) 503, 750, 917, 1374
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
BstV2I GAAGAC 5 cut(s) 336, 895, 1446, 2154, 2345
BstX2I RGATCY 4 cut(s) 1579, 2704, 3005, 3365
BstXI CCANNNNNNTGG 1 cut(s) 3064
BstYI RGATCY 4 cut(s) 1579, 2704, 3005, 3365
BsuRI GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
BtgI CCRYGG 2 cut(s) 858, 2971
BtgZI GCGATG 1 cut(s) 153
BtsI GCAGTG 5 cut(s) 220, 261, 500, 3558, 3595
BtsIMutI CAGTG 6 cut(s) 220, 261, 500, 2149, 3558, 3595
Cac8I GCNNGC 2 cut(s) 1409, 1465
CaiI CAGNNNCTG 1 cut(s) 3181
CciI TCATGA 3 cut(s) 643, 1012, 2929
CfoI GCGC 2 cut(s) 138, 2850
Cfr10I RCCGGY 2 cut(s) 1223, 3584
Cfr13I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
Csp6I GTAC 6 cut(s) 94, 458, 522, 1477, 1959, 3740
CviQI GTAC 6 cut(s) 94, 458, 522, 1477, 1959, 3740
DraI TTTAAA 2 cut(s) 249, 3025
DraIII CACNNNGTG 1 cut(s) 2501
EaeI YGGCCR 2 cut(s) 1846, 2392
Eam1104I CTCTTC 8 cut(s) 783, 1100, 1670, 1975, 2336, 3137, 3295, 3309
EarI CTCTTC 8 cut(s) 783, 1100, 1670, 1975, 2336, 3137, 3295, 3309
Ecl136II GAGCTC 1 cut(s) 2158
Eco130I CCWWGG 2 cut(s) 858, 1446
Eco24I GRGCYC 1 cut(s) 2160
Eco32I GATATC 1 cut(s) 2206
Eco47I GGWCC 4 cut(s) 941, 1268, 2648, 3178
Eco53kI GAGCTC 1 cut(s) 2158
Eco57I CTGAAG 5 cut(s) 1218, 2022, 2574, 2775, 3591
Eco88I CYCGRG 1 cut(s) 1894
EcoICRI GAGCTC 1 cut(s) 2158
EcoNI CCTNNNNNAGG 1 cut(s) 1857
EcoO109I RGGNCCY 2 cut(s) 2648, 3178
EcoRI GAATTC 1 cut(s) 2221
EcoRII CCWGG 3 cut(s) 48, 1843, 2151
EcoRV GATATC 1 cut(s) 2206
EcoT14I CCWWGG 2 cut(s) 858, 1446
EcoT22I ATGCAT 3 cut(s) 718, 1333, 2129
EcoT38I GRGCYC 1 cut(s) 2160
ErhI CCWWGG 2 cut(s) 858, 1446
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 880, 912, 2081, 2113, 2541, 2573
FaqI GGGAC 3 cut(s) 853, 3519, 3534
FauI CCCGC 3 cut(s) 830, 1228, 2606
FbaI TGATCA 3 cut(s) 622, 1492, 3016
Fnu4HI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
FriOI GRGCYC 1 cut(s) 2160
Fsp4HI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
GlaI GCGC 2 cut(s) 137, 2849
GluI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
GsuI CTGGAG 2 cut(s) 2174, 3171
HaeIII GGCC 5 cut(s) 36, 1848, 2394, 2619, 2947
HapII CCGG 3 cut(s) 154, 1224, 3585
HhaI GCGC 2 cut(s) 138, 2850
Hin6I GCGC 2 cut(s) 136, 2848
HinP1I GCGC 2 cut(s) 136, 2848
HincII GTYRAC 2 cut(s) 1024, 3484
HindII GTYRAC 2 cut(s) 1024, 3484
HindIII AAGCTT 1 cut(s) 2804
HpaI GTTAAC 2 cut(s) 1024, 3484
HpaII CCGG 3 cut(s) 154, 1224, 3585
Hpy166II GTNNAC 7 cut(s) 398, 426, 1024, 1268, 1766, 3107, 3484
Hpy8I GTNNAC 7 cut(s) 398, 426, 1024, 1268, 1766, 3107, 3484
Hpy99I CGWCG 4 cut(s) 59, 62, 65, 939
HpyCH4III ACNGT 2 cut(s) 1911, 2917
HpyCH4IV ACGT 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 289, 552, 1845, 2812
HpySE526I ACGT 1 cut(s) 353
HspAI GCGC 2 cut(s) 136, 2848
Ksp22I TGATCA 3 cut(s) 622, 1492, 3016
KspAI GTTAAC 2 cut(s) 1024, 3484
LmnI GCTCC 4 cut(s) 1450, 2155, 3094, 3152
Lsp1109I GCAGC 6 cut(s) 463, 517, 1388, 1978, 2566, 3127
LweI GCATC 8 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591, 3595
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 8 cut(s) 77, 359, 1043, 1144, 1786, 1964, 2741, 3574
MfeI CAATTG 2 cut(s) 1209, 2734
MflI RGATCY 4 cut(s) 1579, 2704, 3005, 3365
MhlI GDGCHC 3 cut(s) 222, 2160, 2526
MlsI TGGCCA 2 cut(s) 1848, 2394
MluNI TGGCCA 2 cut(s) 1848, 2394
MlyI GAGTC 8 cut(s) 1301, 1436, 1961, 2335, 2814, 2976, 3329, 3556
MmeI TCCRAC 3 cut(s) 1327, 1857, 2823
Mox20I TGGCCA 2 cut(s) 1848, 2394
Mph1103I ATGCAT 3 cut(s) 718, 1333, 2129
MroXI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
MscI TGGCCA 2 cut(s) 1848, 2394
MslI CAYNNNNRTG 2 cut(s) 618, 3164
Msp20I TGGCCA 2 cut(s) 1848, 2394
MspA1I CMGCKG 2 cut(s) 454, 1379
MspCI CTTAAG 4 cut(s) 1424, 2078, 2977, 3110
MspI CCGG 3 cut(s) 154, 1224, 3585
MspR9I CCNGG 4 cut(s) 50, 155, 1845, 2153
MunI CAATTG 2 cut(s) 1209, 2734
Mva1269I GAATGC 2 cut(s) 1168, 1331
MvaI CCWGG 3 cut(s) 50, 1845, 2153
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 289, 552, 1845, 2812
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 858
NlaIV GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
NmeAIII GCCGAG 1 cut(s) 2923
NmuCI GTSAC 4 cut(s) 359, 1964, 2741, 3574
NsiI ATGCAT 3 cut(s) 718, 1333, 2129
NspI RCATGY 4 cut(s) 1411, 2131, 3163, 3512
PaeI GCATGC 1 cut(s) 1411
PagI TCATGA 3 cut(s) 643, 1012, 2929
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 1168, 1331
PdmI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
PfeI GAWTC 9 cut(s) 42, 126, 755, 1402, 2861, 2933, 3188, 3409, 3668
PkrI GCNGC 6 cut(s) 453, 507, 1378, 1993, 2581, 3117
PleI GAGTC 8 cut(s) 1300, 1436, 1961, 2335, 2814, 2975, 3328, 3555
PpsI GAGTC 8 cut(s) 1300, 1436, 1961, 2335, 2814, 2975, 3328, 3555
PpuMI RGGWCCY 2 cut(s) 2648, 3178
PshBI ATTAAT 3 cut(s) 2364, 3239, 3698
PsiI TTATAA 1 cut(s) 1599
Psp124BI GAGCTC 1 cut(s) 2160
Psp5II RGGWCCY 2 cut(s) 2648, 3178
Psp6I CCWGG 3 cut(s) 48, 1843, 2151
PspGI CCWGG 3 cut(s) 48, 1843, 2151
PspN4I GGNNCC 6 cut(s) 1270, 1452, 2618, 2649, 3282, 3367
PspPI GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
PspPPI RGGWCCY 2 cut(s) 2648, 3178
PstI CTGCAG 2 cut(s) 507, 1378
PstNI CAGNNNCTG 1 cut(s) 3181
PsuI RGATCY 4 cut(s) 1579, 2704, 3005, 3365
PvuII CAGCTG 2 cut(s) 454, 1379
RsaI GTAC 6 cut(s) 95, 459, 523, 1478, 1960, 3741
RsaNI GTAC 6 cut(s) 94, 458, 522, 1477, 1959, 3740
RseI CAYNNNNRTG 2 cut(s) 618, 3164
SacI GAGCTC 1 cut(s) 2160
SatI GCNGC 6 cut(s) 452, 506, 1377, 1992, 2580, 3116
Sau96I GGNCC 5 cut(s) 941, 1268, 2617, 2648, 3178
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 8 cut(s) 1301, 1436, 1961, 2335, 2814, 2976, 3329, 3556
ScrFI CCNGG 4 cut(s) 50, 155, 1845, 2153
SduI GDGCHC 3 cut(s) 222, 2160, 2526
SfaNI GCATC 8 cut(s) 1640, 1848, 1924, 2061, 2482, 2566, 2591, 3595
SfcI CTRYAG 4 cut(s) 503, 750, 917, 1374
SinI GGWCC 4 cut(s) 941, 1268, 2648, 3178
SmiMI CAYNNNNRTG 2 cut(s) 618, 3164
SmlI CTYRAG 8 cut(s) 130, 770, 1424, 2078, 2977, 3093, 3110, 3350
SmoI CTYRAG 8 cut(s) 130, 770, 1424, 2078, 2977, 3093, 3110, 3350
SpeI ACTAGT 2 cut(s) 1306, 2668
SphI GCATGC 1 cut(s) 1411
SsiI CCGC 3 cut(s) 837, 1221, 2613
SspI AATATT 2 cut(s) 187, 2375
SstI GAGCTC 1 cut(s) 2160
StyD4I CCNGG 4 cut(s) 48, 153, 1843, 2151
StyI CCWWGG 2 cut(s) 858, 1446
TaaI ACNGT 2 cut(s) 1911, 2917
TaiI ACGT 1 cut(s) 356
TaqI TCGA 9 cut(s) 937, 1295, 1335, 2007, 2452, 2526, 2641, 3407, 3744
TaqII GACCGA 1 cut(s) 1514
TatI WGTACW 3 cut(s) 93, 1476, 1958
TfiI GAWTC 9 cut(s) 42, 126, 755, 1402, 2861, 2933, 3188, 3409, 3668
TscAI CASTG 6 cut(s) 227, 261, 507, 2149, 3565, 3595
TseFI GTSAC 4 cut(s) 359, 1964, 2741, 3574
TseI GCWGC 6 cut(s) 451, 505, 1376, 1991, 2579, 3115
Tsp45I GTSAC 4 cut(s) 359, 1964, 2741, 3574
TspGWI ACGGA 2 cut(s) 78, 2960
TspRI CASTG 6 cut(s) 227, 261, 507, 2149, 3565, 3595
Vha464I CTTAAG 4 cut(s) 1424, 2078, 2977, 3110
VpaK11BI GGWCC 4 cut(s) 941, 1268, 2648, 3178
VspI ATTAAT 3 cut(s) 2364, 3239, 3698
XagI CCTNNNNNAGG 1 cut(s) 1857
XapI RAATTY 9 cut(s) 323, 666, 1924, 2221, 2325, 2717, 2778, 2793, 3516
XceI RCATGY 4 cut(s) 1411, 2131, 3163, 3512
XmnI GAANNNNTTC 3 cut(s) 1008, 1401, 1623
ZrmI AGTACT 1 cut(s) 95
Zsp2I ATGCAT 3 cut(s) 718, 1333, 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.