MD01G1212700.v1.1

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007218
Physical Location & Seq
Reverse (-)
30617125 .. 30625012
7888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1212700.v1.1.491

Sequence Viewer

Length: 3714 bp
ATGGACTTGGAAGGCTTATCGGTAATATGCTGCGATCTGGGGATTGCAGAAGAAGATGACAATGGCAACCGGATCGGCTACTCGAAGGGCAAGTACTGCTTAGATAATCTGAAAGATTTGCTAAGGTTTTTGAGGCGTGATGACCCGCAGAATCGGGATGTTTTTAAGCAAGTCTGTAAATGGAATATTGTAGATCAGCATTTGGTACCTATCATTGAACACTGCCAAGCTGATACCAACTTGGTTTTGAATGCAGTGAAGGTTCTGGTGTTCCTAACTATGCCAGTTGAGCCTTCGTCGAATGATGTTTTGCAGCAGATAGAGTTTCTGTGGAAGTTGAAGTCTTCGATTACATCTAGTGATACTGTTGCAGTTATAGTATCGCTTCTGGAAAGCCCACTGGAAAATTTGGAACGTGATGCATTCACGGAGGATGACTGGAAATTGGTGCAGTTGGTACTTACTTTATTCCGAAACATCTTAGCTGTTCAAGAAATTTCACAGCAGCAGAAGGCTGGAGGAATGGCCAGTCAATTTGTATCACTCAGAGATAGATTTCTAGAACTTTTGTTTCATGAGAACGTGATGGACATAGTTTTAGTCATAACCCAGCATATTGGTGATTCCCGTAGCTACCTCTGTCACGATAACTTGCTTTTATTGGAAATTTTCCATTACATATTCATGGGTCAAGAACCAGAGTTGATCGCAAACGCATATTCAAAAGGTCTTAAGGTGGATGGAAACACCGCTGGTTCCTTAAATAGTCTCAAGTCCATAATGGAGGAAGAAGAAGAAGAGAAAAAAAGACTTTCTAGACTACGTAACATGAGTCGCCATTCACAATTTAGTGGAACATTTACCCAGCTCACCATGGATGGTTCTGTGGCAGTTTTGAAGGGAAGGCCTACTTCTACCTCTTGTAAAACCATGCTTAAACCACATAATCCCCGGGGTCCAGTAAAAAAAATTGCTTGGGATCATGGGACATTGCCTTCAACAAAGGATAAAATTTTAGAATTGCTCCATGATTTTGTAAACCAGTTTTTATCAGGGGGTTACAATGTTCTGATGCAGTCAATTCGTGAGAATATTGAAAAGGAACACCCCTCAATTCAGAAGAGTGATGTTGTTGTCTTCTTTCAAGTTGCTCAGTTTGCTATTTCTTTCCAGTATCACAAGTCTTCAATTTCTAAGCCTAGCATGGGCACAGAAGCAGATCCAACTGAAGCTCCCACTGATAAAGATGCTGATAGCACATTCTTCAAAGGTGATGTTTGCGGACCCATTGCAGCATCAGTGAACGAATCTATGTTTCAACTAGTCATTTCAAAGTGGCGTTATGCATATGATAGCTTGAAGGAAACACATGACTACAAGTTTTTGTCTGCAGCTGGTTCTCTTATGAAAAATATGATTTGCATGCTGGATTTGGTGCTTAAGTTATTGCCAGCAGATTCCAAGGAGCCTCAAACAGCTCGCATACTTCTCTACAAATTATTTTATGATCAGACTGATGAGGGGATGACGCACTTCCTCATAAATTTACTCAAATCATTTGACACTCACAAACAACCCAGAAGTGATCTTGCAGATTTGATAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAACCTTCAAGCAGGCGGAACTTTGAGGGTTTCTAAAAAATCTAGGAAAGTGAGGAAGAAGAAAACACCTATTGAGAAGGAAACAGAAAATAAACTCTTAGAAGAGCATGGTACCATTCAAAAAGAGATTGGCATCTCCAATGAAGAGCAATCAACAGAAGTCAGTGTGACGGAGAACAGAAGCCTAAACACAATTTCCAATGGGAAAGAAGACACCATTATCCCTGATCAGCCTGATGAATGCAAAATATCTTTGTTGGAGACAGAAAAAATTGAAGACAGTCTGGCACAGATCGATCGCAGAGATTCTGACTATGCCAAAGGTGATCTTGGTTATAGCACAGGCGATTCTTCTGCTGATGAGCAGGTAGCTGCAACTGATGAAGTTGATTTTAAGGTGTCAACTTTGATTTCTGCTTTTGCAAGCCACAGCATCATCCAGAAGTTGTGTTGGTTGCTTAAGTTTTATAAGGGTAACTCGACCAGTACCAATCACTACATAGTCAGCATGCTGCGAAGGATAAGTGATGACCTGGGGCTCTCTCCGATGTTGTATCAGTTGTCACTCCTCACTACATTCTATGACATTCTAGCTGAGCAGAAGTCATGTCCATGCAAGGCGTATGAAAATATTGTCGATTTTTTGAAAAGTTTGGTCAGAAAAATGCTTAAAAAAATGAAAAATCAACCCCTACTTTTTGTGGAAGTTCTCTTCTGGAAAACTCGCAAAGAATGCCATTACATTAATGCTGAGTATTTATTGCAAGAGCTTGGCCATTTGAAGAAAGAAACTAGAAACTGGGCAAACAGTTTAGGGGACGATGAAATTGGTCCATCACTGGACAAAGGATGGACAAGTAGAAGCATAGCAGATGCACTTGGTGAAGATGAAGCTGATGTCGTGCTATCTCATGACCTTGGATATGAGAATGATGGAGAAAATTCTGGCAAAGCGGAGGGAGGTACTGCATATATTTCAGATAATGAGACTGATGGGCAAGCTAATTATGACAATGAGGGAAAGTCCATAGAGAATGAAACTGAGAGGGTTTCTAGAAAAAACAAGCGACTTGTTATTGGAGCTGAACTGGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGATGACCAGAATTGTAGTCATCTTATTGCGGAAGCTCTTGATCGTGATGGTAAAGTTTCATCAGCTCAAATTTCTAACAAGCTTAAACAGCTAGGGCTGAAAGTTGCACGAAGGAAAAGAATTCGGAAAGCTCAGGAATCTGTTTCCACTGGGCCTAGTCAAATTGATGGAGATGGAAGAGTAGAGGAAGCAGTTAAAACTCATTCTGAATCAAAAAGCCAGCCTTTGCGAGCAAGAAAAAGGGTCCGTGCCCTCAGTGAAGATCAGGAAGCTAATATCAGAGCTTTATACGAGCAATTTAAAGACCATAAGAAATGCAGCCATATGATTGCAAATGCGATGGATGGAGATGGGAAATTCACAGCTCCTCAAGTTTCTCGTAAGCTTAAGCAGCTTGGTTTGCATATTCCTCGGAAGAAGAGGCCTTCAGGTGGCATGCTGAAGGATGAGGATCATAGTGACTCTAATGCAAACAAAATGCACGACTCTGATGATGAAACACTATTTTCATTAATGAAAAGGGGCAAGAAGGATAATAGCGACGAGTTGCTAGAACAGACTATTGGACGAGAAGCGTTGGAAGATGATTGTGATGATGAAATCCTAAGCTCTGTTCTTAAGAAAACTAGGCGATCCCTTTCCAAGTCCATAGATCAAAACTCCGAAGCCATCTCGATTCAGGGAACAGAAAGCGGCACTGTTTTGGAAAATGAAGTTGACGAAGGTGCTGCTGCGAAACGTGCTAGTCTGAATGGCACAGAACAAGCTGAAGTCACAGGCACCAGCAGTGGTAATCCATTAGATGTTGGACCTGTGAAAACTTTGGAGGATTTGCTACATCAGGAAATGGACACTGATCTGGCAGATTCGGAGGATGAAGTAGGTACATTGCCAGTGAGTGGTGTAAGCAGAAGGAGGTCAAGGATGGTACTTGATGATGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1238

Amino Acids

139.15

Weight (kDa)

5.32

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 27 - 288 4.5e-61 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1614, 2103
Acc36I ACCTGC 1 cut(s) 1990
Acc65I GGTACC 2 cut(s) 205, 1745
AccB1I GGYRCC 3 cut(s) 205, 1745, 3539
AccB7I CCANNNNNTGG 1 cut(s) 3659
AciI CCGC 7 cut(s) 146, 750, 1281, 1650, 2588, 2789, 3453
AclWI GGATC 6 cut(s) 80, 987, 1214, 2748, 3219, 3387
AcoI YGGCCR 2 cut(s) 525, 2407
AcuI CTGAAG 4 cut(s) 1248, 3171, 3221, 3549
AdeI CACNNNGTG 1 cut(s) 2516
AfaI GTAC 8 cut(s) 95, 207, 459, 1747, 2122, 2599, 3646, 3690
AfiI CCNNNNNNNGG 3 cut(s) 1205, 3191, 3659
AflII CTTAAG 5 cut(s) 731, 1439, 2093, 3146, 3377
AhlI ACTAGT 1 cut(s) 1321
AjnI CCWGG 1 cut(s) 2166
AjuI GAANNNNNNNTTGG 2 cut(s) 3306, 3338
Alw26I GTCTC 3 cut(s) 773, 1889, 2615
AlwI GGATC 6 cut(s) 80, 987, 1214, 2748, 3219, 3387
Ama87I CYCGRG 1 cut(s) 951
AoxI GGCC 5 cut(s) 525, 905, 2407, 2912, 3182
AseI ATTAAT 2 cut(s) 2379, 3272
Asp700I GAANNNNTTC 1 cut(s) 1638
Asp718I GGTACC 2 cut(s) 205, 1745
AspS9I GGNCC 6 cut(s) 956, 1283, 2465, 2912, 3004, 3569
AsuC2I CCSGG 2 cut(s) 952, 953
AsuHPI GGTGA 5 cut(s) 632, 862, 1283, 1970, 2528
AvaI CYCGRG 1 cut(s) 951
AvaII GGWCC 5 cut(s) 956, 1283, 2465, 3004, 3569
BaeGI GKGCMC 2 cut(s) 1211, 3013
BalI TGGCCA 2 cut(s) 527, 2409
BanI GGYRCC 3 cut(s) 205, 1745, 3539
BanII GRGCYC 1 cut(s) 2175
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 5 cut(s) 336, 1129, 1176, 1851, 1917
BceAI ACGGC 1 cut(s) 1640
BciT130I CCWGG 1 cut(s) 2168
BclI TGATCA 2 cut(s) 1507, 1861
BcnI CCSGG 2 cut(s) 952, 953
BcoDI GTCTC 3 cut(s) 773, 1889, 2615
BcuI ACTAGT 1 cut(s) 1321
BfmI CTRYAG 1 cut(s) 1389
BfrI CTTAAG 5 cut(s) 731, 1439, 2093, 3146, 3377
BfuAI ACCTGC 1 cut(s) 1990
BlpI GCTNAGC 1 cut(s) 2229
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 3 cut(s) 952, 953, 2168
Bme18I GGWCC 5 cut(s) 956, 1283, 2465, 3004, 3569
BmeT110I CYCGRG 1 cut(s) 951
BmgT120I GGNCC 6 cut(s) 956, 1283, 2465, 2912, 3004, 3569
BmiI GGNNCC 8 cut(s) 207, 757, 957, 1285, 1467, 1747, 3005, 3541
BmrFI CCNGG 3 cut(s) 952, 953, 2168
BmrI ACTGGG 2 cut(s) 2443, 2919
BmsI GCATC 7 cut(s) 409, 1062, 1237, 1304, 1776, 2076, 2497
BmuI ACTGGG 2 cut(s) 2443, 2919
BpiI GAAGAC 5 cut(s) 336, 1129, 1176, 1851, 1917
BpmI CTGGAG 2 cut(s) 537, 2744
Bpu10I CCTNAGC 3 cut(s) 122, 2892, 3365
Bpu1102I GCTNAGC 1 cut(s) 2229
BpuEI CTTGAG 2 cut(s) 755, 3114
BpuMI CCSGG 2 cut(s) 952, 953
Bsa29I ATCGAT 1 cut(s) 1929
BsaAI YACGTR 1 cut(s) 824
BsaBI GATNNNNATC 2 cut(s) 1766, 3210
BsaJI CCNNGG 8 cut(s) 873, 950, 951, 952, 1461, 2167, 2551, 3170
BsaWI WCCGGW 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 1216, 1246
Bsc4I CCNNNNNNNGG 3 cut(s) 1205, 3191, 3659
Bse3DI GCAATG 3 cut(s) 989, 1287, 3647
Bse8I GATNNNNATC 2 cut(s) 1766, 3210
BseBI CCWGG 1 cut(s) 2168
BseCI ATCGAT 1 cut(s) 1929
BseDI CCNNGG 8 cut(s) 873, 950, 951, 952, 1461, 2167, 2551, 3170
BseJI GATNNNNATC 2 cut(s) 1766, 3210
BseLI CCNNNNNNNGG 3 cut(s) 1205, 3191, 3659
BseMI GCAATG 3 cut(s) 989, 1287, 3647
BseMII CTCAG 7 cut(s) 559, 1166, 2220, 2376, 2667, 2906, 3028
BseRI GAGGAG 2 cut(s) 2192, 3117
BseSI GKGCMC 2 cut(s) 1211, 3013
BseYI CCCAGC 2 cut(s) 609, 864
BsgI GTGCAG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 1933
BshFI GGCC 5 cut(s) 527, 907, 2409, 2914, 3184
BshNI GGYRCC 3 cut(s) 205, 1745, 3539
BshVI ATCGAT 1 cut(s) 1929
BsiEI CGRYCG 1 cut(s) 1933
BsiHKCI CYCGRG 1 cut(s) 951
BsiSI CCGG 2 cut(s) 70, 952
BslFI GGGAC 2 cut(s) 1000, 2465
BslI CCNNNNNNNGG 3 cut(s) 1205, 3191, 3659
BsmAI GTCTC 3 cut(s) 773, 1889, 2615
BsmFI GGGAC 2 cut(s) 1000, 2465
BsmI GAATGC 4 cut(s) 256, 422, 1880, 2372
BsnI GGCC 5 cut(s) 527, 907, 2409, 2914, 3184
BsoBI CYCGRG 1 cut(s) 951
Bsp1286I GDGCHC 3 cut(s) 1211, 2175, 3013
Bsp1720I GCTNAGC 1 cut(s) 2229
Bsp19I CCATGG 1 cut(s) 873
BspACI CCGC 7 cut(s) 146, 750, 1281, 1650, 2588, 2789, 3453
BspANI GGCC 5 cut(s) 527, 907, 2409, 2914, 3184
BspCNI CTCAG 7 cut(s) 558, 1165, 2221, 2377, 2668, 2905, 3027
BspDI ATCGAT 1 cut(s) 1929
BspHI TCATGA 2 cut(s) 574, 2545
BspLI GGNNCC 8 cut(s) 207, 757, 957, 1285, 1467, 1747, 3005, 3541
BspMAI CTGCAG 1 cut(s) 1393
BspMI ACCTGC 1 cut(s) 1990
BspPI GGATC 6 cut(s) 80, 987, 1214, 2748, 3219, 3387
BspQI GCTCTTC 2 cut(s) 1731, 1773
BspT107I GGYRCC 3 cut(s) 205, 1745, 3539
BspTI CTTAAG 5 cut(s) 731, 1439, 2093, 3146, 3377
BsrDI GCAATG 3 cut(s) 989, 1287, 3647
BssECI CCNNGG 8 cut(s) 873, 950, 951, 952, 1461, 2167, 2551, 3170
BssT1I CCWWGG 3 cut(s) 873, 1461, 2551
Bst2UI CCWGG 1 cut(s) 2168
Bst4CI ACNGT 4 cut(s) 367, 1916, 2444, 3460
Bst6I CTCTTC 7 cut(s) 792, 1115, 1731, 1773, 2351, 2932, 3173
BstAFI CTTAAG 5 cut(s) 731, 1439, 2093, 3146, 3377
BstAPI GCANNNNNTGC 2 cut(s) 96, 2367
BstBAI YACGTR 1 cut(s) 824
BstDSI CCRYGG 1 cut(s) 873
BstMAI GTCTC 3 cut(s) 773, 1889, 2615
BstMCI CGRYCG 1 cut(s) 1933
BstMWI GCNNNNNNNGC 8 cut(s) 96, 289, 1157, 2367, 2848, 3151, 3160, 3500
BstNI CCWGG 1 cut(s) 2168
BstNSI RCATGY 3 cut(s) 1426, 2146, 3199
BstSCI CCNGG 3 cut(s) 950, 951, 2166
BstSFI CTRYAG 1 cut(s) 1389
BstSLI GKGCMC 2 cut(s) 1211, 3013
BstSNI TACGTA 1 cut(s) 824
BstV2I GAAGAC 5 cut(s) 336, 1129, 1176, 1851, 1917
BstX2I RGATCY 2 cut(s) 1219, 2740
BstXI CCANNNNNNTGG 1 cut(s) 617
BstYI RGATCY 2 cut(s) 1219, 2740
Bsu15I ATCGAT 1 cut(s) 1929
BsuRI GGCC 5 cut(s) 527, 907, 2409, 2914, 3184
BsuTUI ATCGAT 1 cut(s) 1929
BtgI CCRYGG 1 cut(s) 873
BtgZI GCGATG 1 cut(s) 3113
BtsI GCAGTG 3 cut(s) 220, 261, 3553
BveI ACCTGC 1 cut(s) 1990
CciI TCATGA 2 cut(s) 574, 2545
Cfr13I GGNCC 6 cut(s) 956, 1283, 2465, 2912, 3004, 3569
Cfr9I CCCGGG 1 cut(s) 951
ClaI ATCGAT 1 cut(s) 1929
CseI GACGC 1 cut(s) 1537
Csp6I GTAC 8 cut(s) 94, 206, 458, 1746, 2121, 2598, 3645, 3689
CviQI GTAC 8 cut(s) 94, 206, 458, 1746, 2121, 2598, 3645, 3689
DraI TTTAAA 1 cut(s) 3061
DraIII CACNNNGTG 1 cut(s) 2516
EaeI YGGCCR 2 cut(s) 525, 2407
Eam1104I CTCTTC 7 cut(s) 792, 1115, 1731, 1773, 2351, 2932, 3173
EarI CTCTTC 7 cut(s) 792, 1115, 1731, 1773, 2351, 2932, 3173
EciI GGCGGA 1 cut(s) 1665
Eco105I TACGTA 1 cut(s) 824
Eco130I CCWWGG 3 cut(s) 873, 1461, 2551
Eco147I AGGCCT 2 cut(s) 907, 3184
Eco24I GRGCYC 1 cut(s) 2175
Eco47I GGWCC 5 cut(s) 956, 1283, 2465, 3004, 3569
Eco57I CTGAAG 4 cut(s) 1248, 3171, 3221, 3549
Eco88I CYCGRG 1 cut(s) 951
EcoRI GAATTC 1 cut(s) 2880
EcoRII CCWGG 1 cut(s) 2166
EcoT14I CCWWGG 3 cut(s) 873, 1461, 2551
EcoT22I ATGCAT 2 cut(s) 424, 1348
EcoT38I GRGCYC 1 cut(s) 2175
ErhI CCWWGG 3 cut(s) 873, 1461, 2551
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 895, 927, 1947, 1979, 2968, 3000
FaqI GGGAC 2 cut(s) 1000, 2465
FauI CCCGC 1 cut(s) 153
FauNDI CATATG 2 cut(s) 1348, 3084
FbaI TGATCA 2 cut(s) 1507, 1861
FriOI GRGCYC 1 cut(s) 2175
GsaI CCCAGC 2 cut(s) 613, 868
GsuI CTGGAG 2 cut(s) 537, 2744
HaeIII GGCC 5 cut(s) 527, 907, 2409, 2914, 3184
HapII CCGG 2 cut(s) 70, 952
HgaI GACGC 1 cut(s) 1537
HincII GTYRAC 2 cut(s) 2037, 3478
HindII GTYRAC 2 cut(s) 2037, 3478
HindIII AAGCTT 2 cut(s) 2840, 3143
HpaII CCGG 2 cut(s) 70, 952
HphI GGTGA 5 cut(s) 632, 862, 1283, 1970, 2528
Hpy166II GTNNAC 4 cut(s) 1039, 1303, 2037, 3478
Hpy8I GTNNAC 4 cut(s) 1039, 1303, 2037, 3478
Hpy99I CGWCG 2 cut(s) 301, 3305
HpyCH4III ACNGT 4 cut(s) 367, 1916, 2444, 3460
HpyCH4IV ACGT 4 cut(s) 415, 582, 823, 3499
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 289, 1157, 2367, 2848, 3151, 3160, 3500
HpySE526I ACGT 4 cut(s) 415, 582, 823, 3499
KpnI GGTACC 2 cut(s) 209, 1749
Ksp22I TGATCA 2 cut(s) 1507, 1861
LguI GCTCTTC 2 cut(s) 1731, 1773
LmnI GCTCC 5 cut(s) 1029, 1237, 1465, 2714, 3130
LweI GCATC 7 cut(s) 409, 1062, 1237, 1304, 1776, 2076, 2497
MaeII ACGT 4 cut(s) 415, 582, 823, 3499
MaeIII GTNAC 8 cut(s) 641, 824, 1058, 1801, 2108, 2196, 3218, 3532
MflI RGATCY 2 cut(s) 1219, 2740
MhlI GDGCHC 3 cut(s) 1211, 2175, 3013
MlsI TGGCCA 2 cut(s) 527, 2409
MluNI TGGCCA 2 cut(s) 527, 2409
MlyI GAGTC 3 cut(s) 841, 3215, 3239
MmeI TCCRAC 4 cut(s) 1247, 1872, 3318, 3547
Mox20I TGGCCA 2 cut(s) 527, 2409
Mph1103I ATGCAT 2 cut(s) 424, 1348
MroXI GAANNNNTTC 1 cut(s) 1638
MscI TGGCCA 2 cut(s) 527, 2409
MslI CAYNNNNRTG 3 cut(s) 618, 683, 2245
Msp20I TGGCCA 2 cut(s) 527, 2409
MspA1I CMGCKG 2 cut(s) 752, 1394
MspCI CTTAAG 5 cut(s) 731, 1439, 2093, 3146, 3377
MspI CCGG 2 cut(s) 70, 952
MspR9I CCNGG 3 cut(s) 952, 953, 2168
Mva1269I GAATGC 4 cut(s) 256, 422, 1880, 2372
MvaI CCWGG 1 cut(s) 2168
MwoI GCNNNNNNNGC 8 cut(s) 96, 289, 1157, 2367, 2848, 3151, 3160, 3500
NciI CCSGG 2 cut(s) 952, 953
NcoI CCATGG 1 cut(s) 873
NdeI CATATG 2 cut(s) 1348, 3084
NlaIV GGNNCC 8 cut(s) 207, 757, 957, 1285, 1467, 1747, 3005, 3541
NmuCI GTSAC 5 cut(s) 641, 1801, 2196, 3218, 3532
NsiI ATGCAT 2 cut(s) 424, 1348
NspI RCATGY 3 cut(s) 1426, 2146, 3199
PaeI GCATGC 3 cut(s) 1426, 2146, 3199
PagI TCATGA 2 cut(s) 574, 2545
PceI AGGCCT 2 cut(s) 907, 3184
PciSI GCTCTTC 2 cut(s) 1731, 1773
PctI GAATGC 4 cut(s) 256, 422, 1880, 2372
PdmI GAANNNNTTC 1 cut(s) 1638
PflMI CCANNNNNTGG 1 cut(s) 3659
Ple19I CGATCG 1 cut(s) 1933
PleI GAGTC 3 cut(s) 840, 3215, 3239
PpsI GAGTC 3 cut(s) 840, 3215, 3239
Ppu21I YACGTR 1 cut(s) 824
PshBI ATTAAT 2 cut(s) 2379, 3272
PsiI TTATAA 2 cut(s) 1614, 2103
Psp6I CCWGG 1 cut(s) 2166
PspFI CCCAGC 2 cut(s) 609, 864
PspGI CCWGG 1 cut(s) 2166
PspN4I GGNNCC 8 cut(s) 207, 757, 957, 1285, 1467, 1747, 3005, 3541
PspPI GGNCC 6 cut(s) 956, 1283, 2465, 2912, 3004, 3569
PstI CTGCAG 1 cut(s) 1393
PsuI RGATCY 2 cut(s) 1219, 2740
PvuI CGATCG 1 cut(s) 1933
PvuII CAGCTG 1 cut(s) 1394
RsaI GTAC 8 cut(s) 95, 207, 459, 1747, 2122, 2599, 3646, 3690
RsaNI GTAC 8 cut(s) 94, 206, 458, 1746, 2121, 2598, 3645, 3689
RseI CAYNNNNRTG 3 cut(s) 618, 683, 2245
SapI GCTCTTC 2 cut(s) 1731, 1773
Sau96I GGNCC 6 cut(s) 956, 1283, 2465, 2912, 3004, 3569
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 3 cut(s) 841, 3215, 3239
ScrFI CCNGG 3 cut(s) 952, 953, 2168
SduI GDGCHC 3 cut(s) 1211, 2175, 3013
SfaNI GCATC 7 cut(s) 409, 1062, 1237, 1304, 1776, 2076, 2497
SfcI CTRYAG 1 cut(s) 1389
SinI GGWCC 5 cut(s) 956, 1283, 2465, 3004, 3569
SmaI CCCGGG 1 cut(s) 953
SmiMI CAYNNNNRTG 3 cut(s) 618, 683, 2245
SmlI CTYRAG 7 cut(s) 731, 770, 1439, 2093, 3129, 3146, 3377
SmoI CTYRAG 7 cut(s) 731, 770, 1439, 2093, 3129, 3146, 3377
SnaBI TACGTA 1 cut(s) 824
SpeI ACTAGT 1 cut(s) 1321
SphI GCATGC 3 cut(s) 1426, 2146, 3199
SseBI AGGCCT 2 cut(s) 907, 3184
SsiI CCGC 7 cut(s) 146, 750, 1281, 1650, 2588, 2789, 3453
SspI AATATT 3 cut(s) 187, 1093, 2266
StuI AGGCCT 2 cut(s) 907, 3184
StyD4I CCNGG 3 cut(s) 950, 951, 2166
StyI CCWWGG 3 cut(s) 873, 1461, 2551
TaaI ACNGT 4 cut(s) 367, 1916, 2444, 3460
TaiI ACGT 4 cut(s) 418, 585, 826, 3502
TaqI TCGA 7 cut(s) 83, 299, 347, 1929, 2114, 2271, 3434
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 3456
TseFI GTSAC 5 cut(s) 641, 1801, 2196, 3218, 3532
Tsp45I GTSAC 5 cut(s) 641, 1801, 2196, 3218, 3532
TspGWI ACGGA 3 cut(s) 443, 1820, 2996
TspMI CCCGGG 1 cut(s) 951
Van91I CCANNNNNTGG 1 cut(s) 3659
Vha464I CTTAAG 5 cut(s) 731, 1439, 2093, 3146, 3377
VpaK11BI GGWCC 5 cut(s) 956, 1283, 2465, 3004, 3569
VspI ATTAAT 2 cut(s) 2379, 3272
XbaI TCTAGA 3 cut(s) 559, 815, 2687
XceI RCATGY 3 cut(s) 1426, 2146, 3199
XmaI CCCGGG 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 1638
ZrmI AGTACT 1 cut(s) 95
Zsp2I ATGCAT 2 cut(s) 424, 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.