AT5G53860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007332
Physical Location & Seq
Forward (+)
21865069 .. 21868948
3880 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53860.2

Sequence Viewer

Length: 1209 bp
ATGTCGAGAGGACCAGGTCGTTTAATACAGAACGTGACACAGTTCGCTGATGCTCAATTCAAACAATTCTCTACTCGTTATGGACAACAAGTTATAGACATTCTCGATTTTCCAATCAAGCTCGTCTTGTCTCCTTTCACTCTCGCCTTTGATATCGCTGGCTCTGCACCTCGTGGTTTTGGCATCCCTGAGTTCATCTCCAAAATTTCGTATCTCTCTGTTTTTGCGGTTGCTACGCTTGGAACTTATGACATTGCATTGGATTTGGGAAAGAAAGTCATATGCCAAAGGGATTGCAAAACTTGTAATGGGTGGCAGGCATTACGGTGCACTATGTGCAAAGGAACAGGGAGTGTTCATTACCAAATCAAAGATTACAACTTGAGAAGTGGAGAGAAACCAACAGCAGATTGTGTCGCAGATGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTCCTCCTTCAACCATAGTGCTCCATTGCCATCAAAAGACTGCCCAACTTGTGATGGAACGATCATGGAACCTCCATGGAAAGCCTATAATGTGCTGAAGAAGATGGATTATCCGTACGAGCACATTGTTCACAGCATGAAAGATCCCAGCATTGCAAATTTCTGGTTGATCACTTTGCCTCAGATTGTGGGTGGGTTTGATTATGATGAAGATGTCAAGAAGAAAATATGGTGGCAATATGAGGAATCTATGCGATATGATCAACTTCGGGACTTGGTGGCTAAACGCAATCCTGGCTGGGAATATTTGCAGGATGCCTTGGTCTCCATTGATCCTGTCCGTGCTCGGGAAGACCCCGTCATAGTGAAGAATGTCCCTTATTACAAGGCTAAGAAATCTCTGGAGGCTGAAGTCACGAAACTCAATCCACCTCCCCGTCCTCAGAATTGGGGTGAACTGAATCTCCCATTGAATATTTCCTCTTGGAGTGAAGAGGATCTCAAAAACCCGGCAAAACTGTACGAGAAGACAGTTCTTCTCAATGCTCAAAGAGAAATCGCAGACAAAATCTTGGATGCACAATGGGAAGCTAAATGGCGTCAAGAGAAGGTGGAGGAAATGTTGGAGCAGAAAGTGAGACCTTACATCCAAGACTCAAGCATGGCTGTTCTTCCACAACCCATCTTGTTAAAGTCACAGAAGAAAGCTCAAAAGGGAAGCCGGCAAAGGAAGTGGTGGTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

46.15

Weight (kDa)

8.58

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AtTam37 PF25112 90 - 200 1.4e-61 AtTam37 zinc finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 227
AclWI GGATC 3 cut(s) 599, 788, 966
AcsI RAATTY 2 cut(s) 204, 619
AcuI CTGAAG 2 cut(s) 578, 891
AcyI GRCGYC 1 cut(s) 1060
AdeI CACNNNGTG 2 cut(s) 173, 336
AfaI GTAC 2 cut(s) 578, 983
AfiI CCNNNNNNNGG 1 cut(s) 808
AgsI TTSAA 3 cut(s) 61, 472, 934
AjnI CCWGG 2 cut(s) 13, 754
AloI GAACNNNNNNTCC 2 cut(s) 909, 941
AluBI AGCT 4 cut(s) 121, 443, 1052, 1169
AluI AGCT 4 cut(s) 121, 443, 1052, 1169
Alw21I GWGCWC 4 cut(s) 332, 484, 585, 808
Alw26I GTCTC 3 cut(s) 135, 790, 1093
Alw44I GTGCAC 1 cut(s) 328
AlwI GGATC 3 cut(s) 599, 788, 966
Ama87I CYCGRG 1 cut(s) 807
ApaLI GTGCAC 1 cut(s) 328
ApoI RAATTY 2 cut(s) 204, 619
AspS9I GGNCC 1 cut(s) 11
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 1 cut(s) 926
AvaI CYCGRG 1 cut(s) 807
AvaII GGWCC 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 332
BauI CACGAG 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 819, 995
Bbv12I GWGCWC 4 cut(s) 332, 484, 585, 808
BccI CCATC 4 cut(s) 499, 509, 559, 1151
BciT130I CCWGG 2 cut(s) 15, 756
BclI TGATCA 2 cut(s) 630, 721
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 3 cut(s) 135, 790, 1093
BfmI CTRYAG 1 cut(s) 426
Bme1390I CCNGG 3 cut(s) 15, 756, 971
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 807
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 3 cut(s) 15, 756, 971
BmsI GCATC 5 cut(s) 40, 192, 412, 766, 1027
BpiI GAAGAC 2 cut(s) 819, 995
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 1 cut(s) 884
BpuEI CTTGAG 2 cut(s) 403, 1102
BpuMI CCSGG 1 cut(s) 971
BsaHI GRCGYC 1 cut(s) 1060
BsaI GGTCTC 2 cut(s) 790, 1093
BsaJI CCNNGG 2 cut(s) 536, 780
BsaXI ACNNNNNCTCC 4 cut(s) 909, 939, 1067, 1097
Bsc4I CCNNNNNNNGG 1 cut(s) 808
Bse118I RCCGGY 1 cut(s) 1182
Bse3DI GCAATG 3 cut(s) 252, 485, 612
BseBI CCWGG 2 cut(s) 15, 756
BseDI CCNNGG 2 cut(s) 536, 780
BseGI GGATG 4 cut(s) 183, 781, 1042, 1107
BseLI CCNNNNNNNGG 1 cut(s) 808
BseMI GCAATG 3 cut(s) 252, 485, 612
BseMII CTCAG 4 cut(s) 180, 435, 656, 917
BseRI GAGGAG 1 cut(s) 454
BseSI GKGCMC 1 cut(s) 332
BseYI CCCAGC 2 cut(s) 608, 759
BsgI GTGCAG 1 cut(s) 150
BsiHKAI GWGCWC 4 cut(s) 332, 484, 585, 808
BsiHKCI CYCGRG 1 cut(s) 807
BsiSI CCGG 2 cut(s) 971, 1183
BsiWI CGTACG 1 cut(s) 576
BslFI GGGAC 2 cut(s) 746, 821
BslI CCNNNNNNNGG 1 cut(s) 808
BsmAI GTCTC 3 cut(s) 135, 790, 1093
BsmFI GGGAC 2 cut(s) 746, 821
Bso31I GGTCTC 2 cut(s) 790, 1093
BsoBI CYCGRG 1 cut(s) 807
Bsp1286I GDGCHC 4 cut(s) 332, 484, 585, 808
Bsp143I GATC 6 cut(s) 522, 604, 630, 721, 793, 958
Bsp19I CCATGG 1 cut(s) 536
BspACI CCGC 1 cut(s) 227
BspCNI CTCAG 4 cut(s) 181, 436, 655, 916
BspLI GGNNCC 1 cut(s) 531
BspPI GGATC 3 cut(s) 599, 788, 966
BspTNI GGTCTC 2 cut(s) 790, 1093
BsrDI GCAATG 3 cut(s) 252, 485, 612
BsrFI RCCGGY 1 cut(s) 1182
BssAI RCCGGY 1 cut(s) 1182
BssECI CCNNGG 2 cut(s) 536, 780
BssMI GATC 6 cut(s) 522, 604, 630, 721, 793, 958
BssNI GRCGYC 1 cut(s) 1060
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 2 cut(s) 536, 780
Bst2BI CACGAG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 15, 756
Bst4CI ACNGT 4 cut(s) 42, 327, 981, 994
Bst6I CTCTTC 1 cut(s) 948
BstACI GRCGYC 1 cut(s) 1060
BstAPI GCANNNNNTGC 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 160, 318, 1184
BstDEI CTNAG 5 cut(s) 189, 444, 642, 852, 903
BstDSI CCRYGG 1 cut(s) 536
BstF5I GGATG 4 cut(s) 183, 781, 1042, 1107
BstKTI GATC 6 cut(s) 525, 607, 633, 724, 796, 961
BstMAI GTCTC 3 cut(s) 135, 790, 1093
BstMBI GATC 6 cut(s) 522, 604, 630, 721, 793, 958
BstMWI GCNNNNNNNGC 3 cut(s) 164, 336, 756
BstNI CCWGG 2 cut(s) 15, 756
BstSCI CCNGG 3 cut(s) 13, 754, 969
BstSFI CTRYAG 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 332
BstV2I GAAGAC 2 cut(s) 819, 995
BstX2I RGATCY 2 cut(s) 604, 958
BstYI RGATCY 2 cut(s) 604, 958
BtgI CCRYGG 1 cut(s) 536
BtsCI GGATG 4 cut(s) 183, 781, 1042, 1107
Cac8I GCNNGC 3 cut(s) 160, 318, 1184
Cfr10I RCCGGY 1 cut(s) 1182
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 1 cut(s) 1049
CsiI ACCWGGT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 577, 982
CspCI CAANNNNNGTGG 1 cut(s) 1175
CviAII CATG 4 cut(s) 526, 537, 598, 1123
CviQI GTAC 2 cut(s) 577, 982
DdeI CTNAG 5 cut(s) 189, 444, 642, 852, 903
DpnI GATC 6 cut(s) 524, 606, 632, 723, 795, 960
DpnII GATC 6 cut(s) 522, 604, 630, 721, 793, 958
DraIII CACNNNGTG 2 cut(s) 173, 336
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
Eco130I CCWWGG 2 cut(s) 536, 780
Eco31I GGTCTC 2 cut(s) 790, 1093
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 2 cut(s) 578, 891
Eco88I CYCGRG 1 cut(s) 807
EcoRII CCWGG 2 cut(s) 13, 754
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 536, 780
ErhI CCWWGG 2 cut(s) 536, 780
FaeI CATG 4 cut(s) 529, 540, 601, 1126
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 2 cut(s) 746, 821
FatI CATG 4 cut(s) 525, 536, 597, 1122
FauNDI CATATG 1 cut(s) 281
FbaI TGATCA 2 cut(s) 630, 721
FokI GGATG 4 cut(s) 170, 788, 1049, 1094
GsaI CCCAGC 2 cut(s) 612, 763
GsuI CTGGAG 1 cut(s) 884
HapII CCGG 2 cut(s) 971, 1183
HgaI GACGC 1 cut(s) 1049
Hin1I GRCGYC 1 cut(s) 1060
Hin1II CATG 4 cut(s) 529, 540, 601, 1126
HinfI GANTC 4 cut(s) 436, 707, 922, 1115
HpaII CCGG 2 cut(s) 971, 1183
HphI GGTGA 1 cut(s) 926
Hpy166II GTNNAC 3 cut(s) 330, 592, 917
Hpy188I TCNGA 2 cut(s) 645, 906
Hpy188III TCNNGA 8 cut(s) 6, 104, 679, 731, 809, 863, 877, 1064
Hpy8I GTNNAC 3 cut(s) 330, 592, 917
HpyAV CCTTC 2 cut(s) 478, 1063
HpyCH4III ACNGT 4 cut(s) 42, 327, 981, 994
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 8 cut(s) 167, 257, 297, 330, 339, 617, 772, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 336, 756
HpyF3I CTNAG 5 cut(s) 189, 444, 642, 852, 903
HpySE526I ACGT 1 cut(s) 33
Hsp92I GRCGYC 1 cut(s) 1060
Hsp92II CATG 4 cut(s) 529, 540, 601, 1126
KroI GCCGGC 1 cut(s) 1182
KroNI GCCGGC 1 cut(s) 1184
Ksp22I TGATCA 2 cut(s) 630, 721
Kzo9I GATC 6 cut(s) 522, 604, 630, 721, 793, 958
LmnI GCTCC 2 cut(s) 487, 1087
LweI GCATC 5 cut(s) 40, 192, 412, 766, 1027
MabI ACCWGGT 1 cut(s) 13
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 3 cut(s) 34, 874, 1155
MalI GATC 6 cut(s) 524, 606, 632, 723, 795, 960
MboI GATC 6 cut(s) 522, 604, 630, 721, 793, 958
MflI RGATCY 2 cut(s) 604, 958
MhlI GDGCHC 4 cut(s) 332, 484, 585, 808
MluCI AATT 5 cut(s) 56, 65, 204, 619, 907
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 1 cut(s) 1065
MroNI GCCGGC 1 cut(s) 1182
MseI TTAA 2 cut(s) 23, 1151
MslI CAYNNNNRTG 1 cut(s) 325
MspI CCGG 2 cut(s) 971, 1183
MspR9I CCNGG 3 cut(s) 15, 756, 971
MvaI CCWGG 2 cut(s) 15, 756
MwoI GCNNNNNNNGC 3 cut(s) 164, 336, 756
NaeI GCCGGC 1 cut(s) 1184
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 536
NdeI CATATG 1 cut(s) 281
NdeII GATC 6 cut(s) 522, 604, 630, 721, 793, 958
NgoMIV GCCGGC 1 cut(s) 1182
NlaIII CATG 4 cut(s) 529, 540, 601, 1126
NlaIV GGNNCC 1 cut(s) 531
NmuCI GTSAC 3 cut(s) 34, 874, 1155
PdiI GCCGGC 1 cut(s) 1184
PfeI GAWTC 3 cut(s) 436, 707, 922
Pfl23II CGTACG 1 cut(s) 576
PflFI GACNNNGTC 2 cut(s) 15, 818
PleI GAGTC 1 cut(s) 1109
PpsI GAGTC 1 cut(s) 1109
Psp6I CCWGG 2 cut(s) 13, 754
PspFI CCCAGC 2 cut(s) 608, 759
PspGI CCWGG 2 cut(s) 13, 754
PspLI CGTACG 1 cut(s) 576
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 2 cut(s) 604, 958
PsyI GACNNNGTC 2 cut(s) 15, 818
RsaI GTAC 2 cut(s) 578, 983
RsaNI GTAC 2 cut(s) 577, 982
RseI CAYNNNNRTG 1 cut(s) 325
SaqAI TTAA 2 cut(s) 23, 1151
Sau3AI GATC 6 cut(s) 522, 604, 630, 721, 793, 958
Sau96I GGNCC 1 cut(s) 11
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 3 cut(s) 15, 756, 971
SduI GDGCHC 4 cut(s) 332, 484, 585, 808
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 5 cut(s) 40, 192, 412, 766, 1027
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 325
SmlI CTYRAG 2 cut(s) 382, 1117
SmoI CTYRAG 2 cut(s) 382, 1117
Sse9I AATT 5 cut(s) 56, 65, 204, 619, 907
SsiI CCGC 1 cut(s) 227
SspI AATATT 2 cut(s) 767, 937
StyD4I CCNGG 3 cut(s) 13, 754, 969
StyI CCWWGG 2 cut(s) 536, 780
TaaI ACNGT 4 cut(s) 42, 327, 981, 994
TaiI ACGT 1 cut(s) 36
TaqI TCGA 3 cut(s) 5, 105, 439
TasI AATT 5 cut(s) 56, 65, 204, 619, 907
TfiI GAWTC 3 cut(s) 436, 707, 922
Tru1I TTAA 2 cut(s) 23, 1151
Tru9I TTAA 2 cut(s) 23, 1151
TseFI GTSAC 3 cut(s) 34, 874, 1155
Tsp45I GTSAC 3 cut(s) 34, 874, 1155
TspDTI ATGAA 5 cut(s) 184, 347, 443, 614, 684
TspGWI ACGGA 2 cut(s) 564, 791
Tth111I GACNNNGTC 2 cut(s) 15, 818
VneI GTGCAC 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 2 cut(s) 204, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.