MD11G1250000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007332
Physical Location & Seq
Forward (+)
36219160 .. 36226588
7429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1250000.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGTCAGGCAGAGACCGTATGCTGAAGAGCCTCAAGAAGTTCGCTGACTTTCATTACAAAATCTTCACGGATAAATATGGCCATCAGCTCACTGACATTTTCGAGTTCCCTATCAAGCTTGTCCTTTCTCCTTTCACTCTTGCTTTCGACATTGCCGGCTCTGCTCAACGTGGGTTTGGCGTTCCCGAGTTGGTGTCCAAGCTATCTTACATGTCAATCTTTGCAGTTGCTACTTTAGGAACTTATGACATAGCATTAGAGATGGGAAAGAAGGTGATATGTCAAAGGAACTGTGGAACCTGCAATGGTTGGCAGGCATTGCGGTGTACCATGTGCAGAGGATCAGGGAGGGTGCAATACCAAGTGAAGAATTACACCTTGAGAAGTGGAGAGAAGGCAACAGCTGAATGTGTTGCAGATGCCATTGCTGATAATCGGGCTGTGTTGGTTCACCTTCCATCCGCCATAAATCTCGATGTGCCCTTGCCATCTAAAGAGTGCCCAACCTGTGATGGCACAGGTGTGATGGGCTGTCCTGAGTGCAAGGACAAATTACAAGTCAGAATCTCTGCAGATGATATCATGGAGCCTCCATGGAAAGCCTACAATGTTTTGAGGAAGATGGACTACCCCTATGAGCATATAGTTCACGGCATGAAAGATCCCAGCATCGCTGCATTTTGGCTGTTTACCTTGCCTCAGGTTGCCGGGGGTTTCGACTTCGATGATGATATCAAGAAAAAAATTTGGTGGCAATACAAGGAATCTATGCGGTATGATCAACTTCGTGATGTGGTGGCCAAGCGAAAACCTGGCTGGGAGGAGTTGCAGGAAGCCTTGATCTCCATAGATCCTGCTCGAGCCAAGGAAGATCCTGTTATTGTGAAAAATATCCCTTACTATAAAGCGAAAAAGGCCCTTGAAACAGAAGTCATGAAACTTGATCCTCCACCAAGGCCGCAAAATTGGGGTGAACTGGACCTTCCACTGAATGCATCTTCTTGGAGCAAGGACGACCTCAAAAATCCTGAAAAGTTCTATGAAATGACTGTTCTTCTAAATGCTCAAAGAGAATTAGCTGACAAAATTTTGGATGCGCAGTGGGAGACTAGATGGCGACAAGAAAGGTTGAATGAGATGTTGGAGGAGAAGGTACGGCCATACATGCAGAGTATTGACAGTGGGACCCTTTCCCAGCCTATTATATTACAATCACAAGATCAGAATCAGAAGAGGACCCGACAACGGAGATGGTGGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

48.56

Weight (kDa)

8.37

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AtTam37 PF25112 89 - 199 1.5e-61 AtTam37 zinc finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1098
Acc36I ACCTGC 1 cut(s) 308
AciI CCGC 4 cut(s) 322, 462, 772, 959
AclWI GGATC 5 cut(s) 349, 656, 845, 866, 938
AcoI YGGCCR 3 cut(s) 79, 798, 1157
AcsI RAATTY 2 cut(s) 744, 1086
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 2 cut(s) 328, 1155
AfiI CCNNNNNNNGG 1 cut(s) 1245
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 2 cut(s) 923, 1132
AjnI CCWGG 1 cut(s) 811
AjuI GAANNNNNNNTTGG 2 cut(s) 1124, 1156
AleI CACNNNNGTG 1 cut(s) 521
AluBI AGCT 5 cut(s) 88, 118, 202, 404, 1079
AluI AGCT 5 cut(s) 88, 118, 202, 404, 1079
Alw26I GTCTC 2 cut(s) 6, 1100
AlwI GGATC 5 cut(s) 349, 656, 845, 866, 938
Ama87I CYCGRG 2 cut(s) 185, 858
AoxI GGCC 5 cut(s) 79, 798, 915, 956, 1157
ApeKI GCWGC 1 cut(s) 674
ApoI RAATTY 2 cut(s) 744, 1086
ArsI GACNNNNNNTTYG 2 cut(s) 543, 575
AspLEI GCGC 1 cut(s) 1099
AspS9I GGNCC 4 cut(s) 916, 979, 1185, 1236
AsuC2I CCSGG 1 cut(s) 709
AsuHPI GGTGA 3 cut(s) 286, 443, 983
AvaI CYCGRG 2 cut(s) 185, 858
AvaII GGWCC 3 cut(s) 979, 1185, 1236
AxyI CCTNAGG 1 cut(s) 699
BaeGI GKGCMC 2 cut(s) 483, 503
BalI TGGCCA 2 cut(s) 81, 800
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BbvI GCAGC 1 cut(s) 661
BccI CCATC 9 cut(s) 90, 256, 466, 496, 506, 520, 616, 1107, 1245
BceAI ACGGC 2 cut(s) 667, 1172
BciT130I CCWGG 1 cut(s) 813
BclI TGATCA 1 cut(s) 778
BcnI CCSGG 1 cut(s) 709
BcoDI GTCTC 2 cut(s) 6, 1100
BfaI CTAG 1 cut(s) 1110
BfmI CTRYAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 675, 959
BlsI GCNGC 2 cut(s) 676, 960
Bme1390I CCNGG 2 cut(s) 709, 813
Bme18I GGWCC 3 cut(s) 979, 1185, 1236
BmeT110I CYCGRG 2 cut(s) 185, 858
BmgT120I GGNCC 4 cut(s) 916, 979, 1185, 1236
BmiI GGNNCC 5 cut(s) 298, 588, 1186, 1187, 1238
BmrFI CCNGG 2 cut(s) 709, 813
BmsI GCATC 4 cut(s) 409, 678, 1004, 1084
BpuEI CTTGAG 2 cut(s) 17, 400
BpuMI CCSGG 1 cut(s) 709
BsaBI GATNNNNATC 1 cut(s) 1224
BsaI GGTCTC 1 cut(s) 6
BsaJI CCNNGG 4 cut(s) 593, 708, 864, 953
Bsc4I CCNNNNNNNGG 1 cut(s) 1245
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 981
Bse21I CCTNAGG 1 cut(s) 699
Bse3DI GCAATG 4 cut(s) 150, 310, 317, 423
Bse8I GATNNNNATC 1 cut(s) 1224
BseBI CCWGG 1 cut(s) 813
BseDI CCNNGG 4 cut(s) 593, 708, 864, 953
BseGI GGATG 2 cut(s) 458, 1099
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 1 cut(s) 1245
BseMI GCAATG 4 cut(s) 150, 310, 317, 423
BseMII CTCAG 2 cut(s) 528, 713
BseNI ACTGG 1 cut(s) 981
BseRI GAGGAG 2 cut(s) 836, 1160
BseSI GKGCMC 2 cut(s) 483, 503
BseXI GCAGC 1 cut(s) 661
BseYI CCCAGC 3 cut(s) 665, 816, 1194
BsgI GTGCAG 1 cut(s) 355
BshFI GGCC 5 cut(s) 81, 800, 917, 958, 1159
BsiHKCI CYCGRG 2 cut(s) 185, 858
BsiSI CCGG 2 cut(s) 156, 708
BslFI GGGAC 1 cut(s) 1198
BslI CCNNNNNNNGG 1 cut(s) 1245
BsmAI GTCTC 2 cut(s) 6, 1100
BsmFI GGGAC 1 cut(s) 1198
BsmI GAATGC 1 cut(s) 997
BsnI GGCC 5 cut(s) 81, 800, 917, 958, 1159
Bso31I GGTCTC 1 cut(s) 6
BsoBI CYCGRG 2 cut(s) 185, 858
Bsp1286I GDGCHC 2 cut(s) 483, 503
Bsp143I GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 4 cut(s) 322, 462, 772, 959
BspANI GGCC 5 cut(s) 81, 800, 917, 958, 1159
BspCNI CTCAG 2 cut(s) 529, 712
BspHI TCATGA 1 cut(s) 933
BspLI GGNNCC 5 cut(s) 298, 588, 1186, 1187, 1238
BspMAI CTGCAG 1 cut(s) 574
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 5 cut(s) 349, 656, 845, 866, 938
BspQI GCTCTTC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 150, 310, 317, 423
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 981
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 4 cut(s) 593, 708, 864, 953
BssMI GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
BssT1I CCWWGG 3 cut(s) 593, 864, 953
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 4 cut(s) 17, 293, 1051, 1181
Bst6I CTCTTC 2 cut(s) 20, 1226
BstAPI GCANNNNNTGC 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 157, 315
BstDEI CTNAG 2 cut(s) 537, 699
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 2 cut(s) 458, 1099
BstHHI GCGC 1 cut(s) 1099
BstKTI GATC 8 cut(s) 344, 664, 781, 843, 853, 874, 946, 1222
BstMAI GTCTC 2 cut(s) 6, 1100
BstMBI GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
BstMWI GCNNNNNNNGC 4 cut(s) 161, 319, 914, 1165
BstNI CCWGG 1 cut(s) 813
BstNSI RCATGY 2 cut(s) 214, 1168
BstSCI CCNGG 2 cut(s) 707, 811
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 2 cut(s) 483, 503
BstV1I GCAGC 1 cut(s) 661
BstX2I RGATCY 3 cut(s) 661, 850, 871
BstYI RGATCY 3 cut(s) 661, 850, 871
Bsu36I CCTNAGG 1 cut(s) 699
BsuRI GGCC 5 cut(s) 81, 800, 917, 958, 1159
BtgI CCRYGG 1 cut(s) 593
BtgZI GCGATG 1 cut(s) 655
BtsCI GGATG 2 cut(s) 458, 1099
BtsI GCAGTG 1 cut(s) 1106
BtsIMutI CAGTG 4 cut(s) 90, 986, 1106, 1186
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 2 cut(s) 157, 315
CciI TCATGA 1 cut(s) 933
CfoI GCGC 1 cut(s) 1099
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 4 cut(s) 916, 979, 1185, 1236
Csp6I GTAC 2 cut(s) 327, 1154
CviAII CATG 7 cut(s) 211, 331, 583, 594, 655, 934, 1165
CviQI GTAC 2 cut(s) 327, 1154
DdeI CTNAG 2 cut(s) 537, 699
DpnI GATC 8 cut(s) 343, 663, 780, 842, 852, 873, 945, 1221
DpnII GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
EaeI YGGCCR 3 cut(s) 79, 798, 1157
Eam1104I CTCTTC 2 cut(s) 20, 1226
EarI CTCTTC 2 cut(s) 20, 1226
EciI GGCGGA 1 cut(s) 451
Eco130I CCWWGG 3 cut(s) 593, 864, 953
Eco31I GGTCTC 1 cut(s) 6
Eco32I GATATC 2 cut(s) 580, 733
Eco47I GGWCC 3 cut(s) 979, 1185, 1236
Eco57I CTGAAG 1 cut(s) 44
Eco81I CCTNAGG 1 cut(s) 699
Eco88I CYCGRG 2 cut(s) 185, 858
EcoO109I RGGNCCY 3 cut(s) 916, 1185, 1236
EcoRII CCWGG 1 cut(s) 811
EcoRV GATATC 2 cut(s) 580, 733
EcoT14I CCWWGG 3 cut(s) 593, 864, 953
EcoT22I ATGCAT 1 cut(s) 997
ErhI CCWWGG 3 cut(s) 593, 864, 953
FaeI CATG 7 cut(s) 214, 334, 586, 597, 658, 937, 1168
FaqI GGGAC 1 cut(s) 1198
FatI CATG 7 cut(s) 210, 330, 582, 593, 654, 933, 1164
FbaI TGATCA 1 cut(s) 778
Fnu4HI GCNGC 2 cut(s) 675, 959
FokI GGATG 2 cut(s) 445, 1106
Fsp4HI GCNGC 2 cut(s) 675, 959
FspBI CTAG 1 cut(s) 1110
FspI TGCGCA 1 cut(s) 1098
GlaI GCGC 1 cut(s) 1098
GluI GCNGC 2 cut(s) 675, 959
GsaI CCCAGC 3 cut(s) 669, 820, 1198
HaeIII GGCC 5 cut(s) 81, 800, 917, 958, 1159
HapII CCGG 2 cut(s) 156, 708
HhaI GCGC 1 cut(s) 1099
Hin1II CATG 7 cut(s) 214, 334, 586, 597, 658, 937, 1168
Hin6I GCGC 1 cut(s) 1097
HinP1I GCGC 1 cut(s) 1097
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 3 cut(s) 564, 764, 1225
HpaII CCGG 2 cut(s) 156, 708
HphI GGTGA 3 cut(s) 286, 443, 983
Hpy166II GTNNAC 5 cut(s) 327, 451, 649, 690, 974
Hpy188I TCNGA 4 cut(s) 563, 1224, 1230, 1265
Hpy188III TCNNGA 8 cut(s) 34, 185, 473, 536, 736, 788, 934, 1028
Hpy8I GTNNAC 5 cut(s) 327, 451, 649, 690, 974
HpyAV CCTTC 5 cut(s) 265, 388, 464, 992, 1144
HpyCH4III ACNGT 4 cut(s) 17, 293, 1051, 1181
HpyCH4IV ACGT 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 319, 914, 1165
HpyF3I CTNAG 2 cut(s) 537, 699
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 7 cut(s) 214, 334, 586, 597, 658, 937, 1168
HspAI GCGC 1 cut(s) 1097
KflI GGGWCCC 1 cut(s) 1185
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 2 cut(s) 586, 1005
Lsp1109I GCAGC 1 cut(s) 661
LweI GCATC 4 cut(s) 409, 678, 1004, 1084
MaeI CTAG 1 cut(s) 1110
MaeII ACGT 1 cut(s) 169
MalI GATC 8 cut(s) 343, 663, 780, 842, 852, 873, 945, 1221
MboI GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
MboII GAAGA 9 cut(s) 37, 55, 379, 631, 881, 990, 1046, 1243, 1252
MflI RGATCY 3 cut(s) 661, 850, 871
MhlI GDGCHC 2 cut(s) 483, 503
MlsI TGGCCA 2 cut(s) 81, 800
MluCI AATT 6 cut(s) 370, 551, 744, 964, 1073, 1086
MluNI TGGCCA 2 cut(s) 81, 800
MmeI TCCRAC 1 cut(s) 1122
Mox20I TGGCCA 2 cut(s) 81, 800
Mph1103I ATGCAT 1 cut(s) 997
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 2 cut(s) 81, 800
MslI CAYNNNNRTG 2 cut(s) 322, 521
Msp20I TGGCCA 2 cut(s) 81, 800
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 156, 708
MspR9I CCNGG 2 cut(s) 709, 813
Mva1269I GAATGC 1 cut(s) 997
MvaI CCWGG 1 cut(s) 813
MwoI GCNNNNNNNGC 4 cut(s) 161, 319, 914, 1165
NaeI GCCGGC 1 cut(s) 157
NciI CCSGG 1 cut(s) 709
NcoI CCATGG 1 cut(s) 593
NdeII GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 7 cut(s) 214, 334, 586, 597, 658, 937, 1168
NlaIV GGNNCC 5 cut(s) 298, 588, 1186, 1187, 1238
NsbI TGCGCA 1 cut(s) 1098
NsiI ATGCAT 1 cut(s) 997
NspI RCATGY 2 cut(s) 214, 1168
OliI CACNNNNGTG 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 858
PagI TCATGA 1 cut(s) 933
PciI ACATGT 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 997
PdiI GCCGGC 1 cut(s) 157
PfeI GAWTC 3 cut(s) 564, 764, 1225
PkrI GCNGC 2 cut(s) 676, 960
PpuMI RGGWCCY 2 cut(s) 1185, 1236
PscI ACATGT 1 cut(s) 210
Psp5II RGGWCCY 2 cut(s) 1185, 1236
Psp6I CCWGG 1 cut(s) 811
PspFI CCCAGC 3 cut(s) 665, 816, 1194
PspGI CCWGG 1 cut(s) 811
PspN4I GGNNCC 5 cut(s) 298, 588, 1186, 1187, 1238
PspPI GGNCC 4 cut(s) 916, 979, 1185, 1236
PspPPI RGGWCCY 2 cut(s) 1185, 1236
PspXI VCTCGAGB 1 cut(s) 858
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 3 cut(s) 661, 850, 871
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 2 cut(s) 328, 1155
RsaNI GTAC 2 cut(s) 327, 1154
RseI CAYNNNNRTG 2 cut(s) 322, 521
SapI GCTCTTC 1 cut(s) 20
SatI GCNGC 2 cut(s) 675, 959
Sau3AI GATC 8 cut(s) 341, 661, 778, 840, 850, 871, 943, 1219
Sau96I GGNCC 4 cut(s) 916, 979, 1185, 1236
ScrFI CCNGG 2 cut(s) 709, 813
SduI GDGCHC 2 cut(s) 483, 503
SfaNI GCATC 4 cut(s) 409, 678, 1004, 1084
SfcI CTRYAG 1 cut(s) 570
Sfr274I CTCGAG 1 cut(s) 858
SinI GGWCC 3 cut(s) 979, 1185, 1236
SlaI CTCGAG 1 cut(s) 858
SmiMI CAYNNNNRTG 2 cut(s) 322, 521
SmlI CTYRAG 3 cut(s) 32, 379, 858
SmoI CTYRAG 3 cut(s) 32, 379, 858
Sse9I AATT 6 cut(s) 370, 551, 744, 964, 1073, 1086
SsiI CCGC 4 cut(s) 322, 462, 772, 959
SspMI CTAG 1 cut(s) 1110
StyD4I CCNGG 2 cut(s) 707, 811
StyI CCWWGG 3 cut(s) 593, 864, 953
TaaI ACNGT 4 cut(s) 17, 293, 1051, 1181
TaiI ACGT 1 cut(s) 172
TaqI TCGA 6 cut(s) 102, 147, 474, 717, 723, 859
TasI AATT 6 cut(s) 370, 551, 744, 964, 1073, 1086
TauI GCSGC 1 cut(s) 961
TfiI GAWTC 3 cut(s) 564, 764, 1225
TscAI CASTG 4 cut(s) 97, 993, 1106, 1186
TseI GCWGC 1 cut(s) 674
TspDTI ATGAA 4 cut(s) 41, 671, 950, 1056
TspGWI ACGGA 2 cut(s) 83, 1261
TspRI CASTG 4 cut(s) 97, 993, 1106, 1186
VpaK11BI GGWCC 3 cut(s) 979, 1185, 1236
XapI RAATTY 2 cut(s) 744, 1086
XceI RCATGY 2 cut(s) 214, 1168
XhoI CTCGAG 1 cut(s) 858
XspI CTAG 1 cut(s) 1110
Zsp2I ATGCAT 1 cut(s) 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.