Rmu_ssc0000299.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000299.1
OG0007332
Physical Location & Seq
Reverse (-)
2 .. 6150
6149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000299.1_g000001.1.cds

Sequence Viewer

Length: 1192 bp
atgtcaagagaccgcctcgtaaagagcgtcaagaagtttgctgatgtccagtacaagctcttcacagctcgctatggtcagcagctcatcgacattttcgagttccccgtaaagcttgtgctctctcctttcactatcgccattgacgttgccggctccgcccaacgtggctttggcgtgcccgagttgatttccaagctctcttacgcctcaatatttgtagtagctactctagggacttatgacatagcgctagagatgggaaagaaggtgatatgccagaggaactgtgaaacctgcaacggttggcaggcattgcgatgtaccttgtgcaaaggatcagggagggtgcactaccaagtgaaaaattacacattgaaaagtggggagagggaaacggctgaatgtgttgcagatgctattgctgacagtcgtgctgagttggttcatcttccatctgccataaatcttcatacaccattgccgtctaaagactgcctgacctgtgatggcacgggtgtaatgagctgtcctgagtgcaaaaacaaattacaagtcagaatctctgcagatgatataatggagcctccctggaaagcctacaatgttatgaagaagatggactacccttatgagcatatagttgacagcatgaaagatcccagcattgctgcattttggctatttaccttgcctcgggttgtgggtggttacgactatgatgatgatgtcaaacaaaaactttggtggcaatacaaggaatctatgcggtatgatcaacttcgtgatgtggtggccaagcgaaaacctggctgggagcacttgcaggaagccttgatctccatagatcctgctcgtgcaagggatgatcctgttattgtaaaaaatatccctttctataaagcgaagaaggcacttgaagcagaagtcatgaaactaaatcctccagcaaggccacaaaattggggtgaactggaccttccattggatgcatcttcctggagcaaggaggatctcaaagatcctggaaagttttatgaaatgaccgttcttcttaatgctcagcgagaaatcgctgacaaaattttggatgcacaatgggagactaaatggcgtcaggaaaagttgaatgagatgttggcggagaaggtacggccatacatgcagaagatcgacagtgggaccctctctc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

397

Amino Acids

45.31

Weight (kDa)

7.91

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 305
AciI CCGC 4 cut(s) 13, 159, 769, 1142
AclWI GGATC 6 cut(s) 346, 653, 842, 863, 1016, 1020
AcoI YGGCCR 2 cut(s) 795, 1154
AcsI RAATTY 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 1114
AfaI GTAC 3 cut(s) 53, 325, 1152
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 2 cut(s) 696, 985
AgsI TTSAA 3 cut(s) 379, 920, 1129
AjnI CCWGG 4 cut(s) 590, 808, 998, 1024
AjuI GAANNNNNNNTTGG 2 cut(s) 1121, 1153
AluBI AGCT 7 cut(s) 58, 68, 85, 115, 199, 227, 528
AluI AGCT 7 cut(s) 58, 68, 85, 115, 199, 227, 528
Alw21I GWGCWC 3 cut(s) 123, 354, 822
Alw26I GTCTC 2 cut(s) 3, 1097
Alw44I GTGCAC 1 cut(s) 350
AlwI GGATC 6 cut(s) 346, 653, 842, 863, 1016, 1020
Ama87I CYCGRG 2 cut(s) 182, 696
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 3 cut(s) 795, 953, 1154
ApaLI GTGCAC 1 cut(s) 350
ApeKI GCWGC 2 cut(s) 82, 671
ApoI RAATTY 1 cut(s) 1083
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 976, 1182
AsuHPI GGTGA 2 cut(s) 283, 980
AvaI CYCGRG 2 cut(s) 182, 696
AvaII GGWCC 2 cut(s) 976, 1182
BaeGI GKGCMC 2 cut(s) 183, 354
BalI TGGCCA 1 cut(s) 797
BarI GAAGNNNNNNTAC 4 cut(s) 44, 76, 608, 640
BauI CACGAG 1 cut(s) 855
Bbv12I GWGCWC 3 cut(s) 123, 354, 822
BbvI GCAGC 2 cut(s) 94, 658
BccI CCATC 4 cut(s) 253, 463, 503, 613
BceAI ACGGC 3 cut(s) 414, 469, 1169
BciT130I CCWGG 4 cut(s) 592, 810, 1000, 1026
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 2 cut(s) 3, 1097
BfaI CTAG 2 cut(s) 233, 254
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 2 cut(s) 83, 672
BlpI GCTNAGC 1 cut(s) 1062
BlsI GCNGC 2 cut(s) 84, 673
Bme1390I CCNGG 4 cut(s) 592, 810, 1000, 1026
Bme18I GGWCC 2 cut(s) 976, 1182
BmeT110I CYCGRG 2 cut(s) 182, 696
BmgT120I GGNCC 2 cut(s) 976, 1182
BmiI GGNNCC 4 cut(s) 157, 585, 1183, 1184
BmrFI CCNGG 4 cut(s) 592, 810, 1000, 1026
BmsI GCATC 4 cut(s) 406, 979, 1001, 1081
BplI GAGNNNNNCTC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 930, 1021
Bpu1102I GCTNAGC 1 cut(s) 1062
BsaHI GRCGYC 1 cut(s) 1114
BsaI GGTCTC 1 cut(s) 3
BsaJI CCNNGG 2 cut(s) 590, 695
Bsc4I CCNNNNNNNGG 2 cut(s) 696, 985
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 49, 978
Bse3DI GCAATG 3 cut(s) 314, 479, 666
BseBI CCWGG 4 cut(s) 592, 810, 1000, 1026
BseDI CCNNGG 2 cut(s) 590, 695
BseGI GGATG 3 cut(s) 871, 994, 1096
BseLI CCNNNNNNNGG 2 cut(s) 696, 985
BseMI GCAATG 3 cut(s) 314, 479, 666
BseMII CTCAG 3 cut(s) 429, 525, 1076
BseNI ACTGG 2 cut(s) 49, 978
BseSI GKGCMC 2 cut(s) 183, 354
BseXI GCAGC 2 cut(s) 94, 658
BseYI CCCAGC 2 cut(s) 662, 813
BshFI GGCC 3 cut(s) 797, 955, 1156
BsiHKAI GWGCWC 3 cut(s) 123, 354, 822
BsiHKCI CYCGRG 2 cut(s) 182, 696
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 696, 985
BsmAI GTCTC 2 cut(s) 3, 1097
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 3 cut(s) 797, 955, 1156
Bso31I GGTCTC 1 cut(s) 3
BsoBI CYCGRG 2 cut(s) 182, 696
Bsp1286I GDGCHC 4 cut(s) 123, 183, 354, 822
Bsp143I GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
Bsp1720I GCTNAGC 1 cut(s) 1062
BspACI CCGC 4 cut(s) 13, 159, 769, 1142
BspANI GGCC 3 cut(s) 797, 955, 1156
BspCNI CTCAG 3 cut(s) 430, 526, 1075
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 4 cut(s) 157, 585, 1183, 1184
BspMAI CTGCAG 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 6 cut(s) 346, 653, 842, 863, 1016, 1020
BspQI GCTCTTC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 3
BsrDI GCAATG 3 cut(s) 314, 479, 666
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 49, 978
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 590, 695
BssMI GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
BssNI GRCGYC 1 cut(s) 1114
BssSI CACGAG 1 cut(s) 855
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 4 cut(s) 592, 810, 1000, 1026
Bst4CI ACNGT 5 cut(s) 290, 305, 431, 1048, 1178
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 1114
BstAPI GCANNNNNTGC 1 cut(s) 316
BstC8I GCNNGC 4 cut(s) 70, 154, 179, 312
BstDEI CTNAG 3 cut(s) 438, 534, 1062
BstF5I GGATG 3 cut(s) 871, 994, 1096
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 9 cut(s) 341, 661, 778, 840, 850, 871, 1015, 1024, 1173
BstMAI GTCTC 2 cut(s) 3, 1097
BstMBI GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
BstMWI GCNNNNNNNGC 5 cut(s) 158, 316, 911, 920, 1162
BstNI CCWGG 4 cut(s) 592, 810, 1000, 1026
BstNSI RCATGY 1 cut(s) 1165
BstSCI CCNGG 4 cut(s) 590, 808, 998, 1024
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 2 cut(s) 183, 354
BstV1I GCAGC 2 cut(s) 94, 658
BstX2I RGATCY 4 cut(s) 658, 847, 1012, 1021
BstXI CCANNNNNNTGG 1 cut(s) 963
BstYI RGATCY 4 cut(s) 658, 847, 1012, 1021
BsuRI GGCC 3 cut(s) 797, 955, 1156
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 3 cut(s) 871, 994, 1096
BtsIMutI CAGTG 1 cut(s) 1183
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 4 cut(s) 70, 154, 179, 312
CciI TCATGA 1 cut(s) 930
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 976, 1182
CseI GACGC 2 cut(s) 16, 1103
Csp6I GTAC 3 cut(s) 52, 324, 1151
CviAII CATG 3 cut(s) 652, 931, 1162
CviQI GTAC 3 cut(s) 52, 324, 1151
DdeI CTNAG 3 cut(s) 438, 534, 1062
DpnI GATC 9 cut(s) 340, 660, 777, 839, 849, 870, 1014, 1023, 1172
DpnII GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
EaeI YGGCCR 2 cut(s) 795, 1154
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 2 cut(s) 148, 1157
Eco31I GGTCTC 1 cut(s) 3
Eco47I GGWCC 2 cut(s) 976, 1182
Eco47III AGCGCT 1 cut(s) 252
Eco88I CYCGRG 2 cut(s) 182, 696
EcoO109I RGGNCCY 1 cut(s) 1182
EcoRII CCWGG 4 cut(s) 590, 808, 998, 1024
EcoT22I ATGCAT 1 cut(s) 994
FaeI CATG 3 cut(s) 655, 934, 1165
FaqI GGGAC 1 cut(s) 250
FatI CATG 3 cut(s) 651, 930, 1161
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 83, 672
FokI GGATG 3 cut(s) 878, 1001, 1103
Fsp4HI GCNGC 2 cut(s) 83, 672
FspBI CTAG 2 cut(s) 233, 254
GlaI GCGC 1 cut(s) 252
GluI GCNGC 2 cut(s) 83, 672
GsaI CCCAGC 2 cut(s) 666, 817
GsuI CTGGAG 2 cut(s) 930, 1021
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 3 cut(s) 797, 955, 1156
HapII CCGG 1 cut(s) 153
HgaI GACGC 2 cut(s) 16, 1103
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 1 cut(s) 1114
Hin1II CATG 3 cut(s) 655, 934, 1165
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 2 cut(s) 561, 761
HpaII CCGG 1 cut(s) 153
HphI GGTGA 2 cut(s) 283, 980
Hpy166II GTNNAC 3 cut(s) 352, 646, 971
Hpy188I TCNGA 1 cut(s) 560
Hpy188III TCNNGA 6 cut(s) 6, 31, 533, 785, 931, 1118
Hpy8I GTNNAC 3 cut(s) 352, 646, 971
HpyAV CCTTC 4 cut(s) 262, 904, 989, 1141
HpyCH4III ACNGT 5 cut(s) 290, 305, 431, 1048, 1178
HpyCH4IV ACGT 2 cut(s) 147, 166
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 316, 911, 920, 1162
HpyF3I CTNAG 3 cut(s) 438, 534, 1062
HpySE526I ACGT 2 cut(s) 147, 166
Hsp92I GRCGYC 1 cut(s) 1114
Hsp92II CATG 3 cut(s) 655, 934, 1165
HspAI GCGC 1 cut(s) 251
KflI GGGWCCC 1 cut(s) 1182
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 4 cut(s) 161, 583, 817, 1002
Lsp1109I GCAGC 2 cut(s) 94, 658
LweI GCATC 4 cut(s) 406, 979, 1001, 1081
MaeI CTAG 2 cut(s) 233, 254
MaeII ACGT 2 cut(s) 147, 166
MaeIII GTNAC 1 cut(s) 710
MalI GATC 9 cut(s) 340, 660, 777, 839, 849, 870, 1014, 1023, 1172
MboI GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
MboII GAAGA 9 cut(s) 52, 443, 461, 625, 628, 919, 987, 1043, 1180
MflI RGATCY 4 cut(s) 658, 847, 1012, 1021
MhlI GDGCHC 4 cut(s) 123, 183, 354, 822
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 4 cut(s) 367, 548, 961, 1083
MluNI TGGCCA 1 cut(s) 797
MnlI CCTC 9 cut(s) 26, 220, 276, 339, 384, 597, 705, 954, 1003
Mox20I TGGCCA 1 cut(s) 797
Mph1103I ATGCAT 1 cut(s) 994
MroNI GCCGGC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 797
MseI TTAA 1 cut(s) 1056
MslI CAYNNNNRTG 1 cut(s) 319
Msp20I TGGCCA 1 cut(s) 797
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 4 cut(s) 592, 810, 1000, 1026
MvaI CCWGG 4 cut(s) 592, 810, 1000, 1026
MwoI GCNNNNNNNGC 5 cut(s) 158, 316, 911, 920, 1162
NaeI GCCGGC 1 cut(s) 154
NdeII GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 3 cut(s) 655, 934, 1165
NlaIV GGNNCC 4 cut(s) 157, 585, 1183, 1184
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 1 cut(s) 1165
PagI TCATGA 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 4 cut(s) 24, 96, 105, 144
PdiI GCCGGC 1 cut(s) 154
PfeI GAWTC 2 cut(s) 561, 761
PfoI TCCNGGA 2 cut(s) 998, 1024
PkrI GCNGC 2 cut(s) 84, 673
PpuMI RGGWCCY 1 cut(s) 1182
Psp5II RGGWCCY 1 cut(s) 1182
Psp6I CCWGG 4 cut(s) 590, 808, 998, 1024
PspFI CCCAGC 2 cut(s) 662, 813
PspGI CCWGG 4 cut(s) 590, 808, 998, 1024
PspN4I GGNNCC 4 cut(s) 157, 585, 1183, 1184
PspPI GGNCC 2 cut(s) 976, 1182
PspPPI RGGWCCY 1 cut(s) 1182
PstI CTGCAG 1 cut(s) 571
PsuI RGATCY 4 cut(s) 658, 847, 1012, 1021
RsaI GTAC 3 cut(s) 53, 325, 1152
RsaNI GTAC 3 cut(s) 52, 324, 1151
RseI CAYNNNNRTG 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 1056
SatI GCNGC 2 cut(s) 83, 672
Sau3AI GATC 9 cut(s) 338, 658, 775, 837, 847, 868, 1012, 1021, 1170
Sau96I GGNCC 2 cut(s) 976, 1182
ScrFI CCNGG 4 cut(s) 592, 810, 1000, 1026
SduI GDGCHC 4 cut(s) 123, 183, 354, 822
SfaNI GCATC 4 cut(s) 406, 979, 1001, 1081
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 2 cut(s) 976, 1182
SmiMI CAYNNNNRTG 1 cut(s) 319
Sse9I AATT 4 cut(s) 367, 548, 961, 1083
SsiI CCGC 4 cut(s) 13, 159, 769, 1142
SspI AATATT 1 cut(s) 216
SspMI CTAG 2 cut(s) 233, 254
StyD4I CCNGG 4 cut(s) 590, 808, 998, 1024
TaaI ACNGT 5 cut(s) 290, 305, 431, 1048, 1178
TaiI ACGT 2 cut(s) 150, 169
TaqI TCGA 3 cut(s) 90, 99, 1173
TasI AATT 4 cut(s) 367, 548, 961, 1083
TatI WGTACW 1 cut(s) 51
TfiI GAWTC 2 cut(s) 561, 761
Tru1I TTAA 1 cut(s) 1056
Tru9I TTAA 1 cut(s) 1056
TscAI CASTG 1 cut(s) 1183
TseI GCWGC 2 cut(s) 82, 671
TspDTI ATGAA 6 cut(s) 437, 461, 626, 668, 947, 1053
TspRI CASTG 1 cut(s) 1183
VneI GTGCAC 1 cut(s) 350
VpaK11BI GGWCC 2 cut(s) 976, 1182
XapI RAATTY 1 cut(s) 1083
XceI RCATGY 1 cut(s) 1165
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XspI CTAG 2 cut(s) 233, 254
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.