Rh5BG230200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007332
Physical Location & Seq
Reverse (-)
27047485 .. 27054792
7308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG230200.1

Sequence Viewer

Length: 1365 bp
ATGACTATCCGGTCTTCCTTTCCAGTTCAATACATTATCCAACTCTCTCTCACTCACCTCTGCAAATTAGAAAGCCAGCCTCTCTCGCTCGCTCTTCCAACAATGTCAAGAGACCGCCTCGTAAAGAGCGTCAAGAAGTTTGCTGATGTCCAATACAAGCTCTTCACAGCTCGCTATGGTCAGCAGCTCATCGACATTTTCGAGTTCCCCGTAAAGCTTGTGCTCTCGCCTTTCACTATCGCCATTGACGTTGCCGGCTCCGCCCAACGTGGCTTTGGCGTGCCCGAGTTGATTTCCAAGCTCTCTTACGCCTCAATATTTGTAGTAGCTACTCTAGGGACTTATGACATAGCGCTAGAGATGGGAAAGAAGGTGATATGCCAGAGGAACTGTGAAACCTGCAACGGTTGGCAGGCATTGCGGTGTACCTTGTGCAAAGGGTCAGGGAGGGTGCACTACCAAGTGAAAAATTACACATTGAAAAGTGGGGAGAGGGAAACGGCTGAATGTGTTGCAAATGCTATTGCTGACAGTCGTGCTGAGTTGGTTCATCTTCCATCTGCCATAAATCTTCATACACCATTGCCGTCTAAAGACTGCCTGACCTGTGATGGCACGGGTGTAATGAGCTGTCCTGAGTGCAAAAACAAATTACAAGTCAGAATCTCTGCAGATGATATAATGGAGCCTCCCTGGAAAGCCTACAATGTTATGAAGAAGATGGACTACCCTTATGAGCATATAGTTGACAGCATGAAAGATCCCAGCATTGCTGCATTTTGGCTATTTACCTTGCCTCGGGTTGTGGGTGGTTACGACTATGATGATGATGTCAAACAAAAACTTTGGTGGCAATACAAGGAATCTATGCGGTATGATCAACTTCGTGATGTGGTGGCCAAGCGAAAACCGGGCTGGGAGCACTTGCAGGAAGCCTTGATCTCCATAGATCCTGCTCGTGCAAGGGATGATCCTGTTATTGTAAAAAATATCCCTTTCTATAAAGCGAAGAAGGCACTTGAAGCAGAAGTCATGAAACTAAATCCTCCAGCAAGGCCACAAAATTGGGGAGAACTGGACCTTCCATTGGATGCATCTTCCTGGAGCAAGGAGGATCTCAAAGATCCTGGAAAGTTTTATGAAATGACCGTTCTTCTTAATGCTCAGCGAGAAATCGCTGACAAAATTTTGGATGCACAATGGGAGACTAAATGGCGTCAAGAAAAGTTGAATGAGATGTTGGCGGAGAAGGTACGGCCATACATGCAGAAGATCGACAGTGGGACCCTCTCTCAGCCTATTATACTACCACCACAAGAACAGAATCAGAAGAGAAGCCGACAACGGAGATGGTGGTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

454

Amino Acids

52.14

Weight (kDa)

8.76

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AtTam37 PF25112 122 - 232 4.4e-61 AtTam37 zinc finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 407
AciI CCGC 5 cut(s) 115, 261, 421, 871, 1244
AclWI GGATC 5 cut(s) 755, 944, 965, 1118, 1122
AcoI YGGCCR 2 cut(s) 897, 1256
AcsI RAATTY 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 1216
AfaI GTAC 2 cut(s) 427, 1254
AfeI AGCGCT 1 cut(s) 354
AfiI CCNNNNNNNGG 2 cut(s) 798, 1087
AgsI TTSAA 4 cut(s) 29, 481, 1022, 1231
AjnI CCWGG 3 cut(s) 692, 1100, 1126
AjuI GAANNNNNNNTTGG 2 cut(s) 1223, 1255
AluBI AGCT 7 cut(s) 160, 170, 187, 217, 301, 329, 630
AluI AGCT 7 cut(s) 160, 170, 187, 217, 301, 329, 630
Alw21I GWGCWC 3 cut(s) 225, 456, 924
Alw26I GTCTC 2 cut(s) 105, 1199
Alw44I GTGCAC 1 cut(s) 452
AlwI GGATC 5 cut(s) 755, 944, 965, 1118, 1122
Ama87I CYCGRG 2 cut(s) 284, 798
Aor51HI AGCGCT 1 cut(s) 354
AoxI GGCC 3 cut(s) 897, 1055, 1256
ApaLI GTGCAC 1 cut(s) 452
ApeKI GCWGC 2 cut(s) 184, 773
ApoI RAATTY 1 cut(s) 1185
ArsI GACNNNNNNTTYG 2 cut(s) 642, 674
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 2 cut(s) 1078, 1284
AsuC2I CCSGG 1 cut(s) 912
AsuHPI GGTGA 2 cut(s) 47, 385
AvaI CYCGRG 2 cut(s) 284, 798
AvaII GGWCC 2 cut(s) 1078, 1284
BaeGI GKGCMC 2 cut(s) 285, 456
BalI TGGCCA 1 cut(s) 899
BarI GAAGNNNNNNTAC 2 cut(s) 710, 742
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 6
Bbv12I GWGCWC 3 cut(s) 225, 456, 924
BbvI GCAGC 2 cut(s) 196, 760
BccI CCATC 5 cut(s) 355, 565, 605, 715, 1344
BceAI ACGGC 3 cut(s) 516, 571, 1271
BciT130I CCWGG 3 cut(s) 694, 1102, 1128
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 912
BcoDI GTCTC 2 cut(s) 105, 1199
BfaI CTAG 2 cut(s) 335, 356
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 407
BisI GCNGC 2 cut(s) 185, 774
BlpI GCTNAGC 1 cut(s) 1164
BlsI GCNGC 2 cut(s) 186, 775
Bme1390I CCNGG 4 cut(s) 694, 912, 1102, 1128
Bme18I GGWCC 2 cut(s) 1078, 1284
BmeT110I CYCGRG 2 cut(s) 284, 798
BmgT120I GGNCC 2 cut(s) 1078, 1284
BmiI GGNNCC 4 cut(s) 259, 687, 1285, 1286
BmrFI CCNGG 4 cut(s) 694, 912, 1102, 1128
BmsI GCATC 3 cut(s) 1081, 1103, 1183
BpiI GAAGAC 1 cut(s) 6
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 2 cut(s) 1032, 1123
Bpu1102I GCTNAGC 1 cut(s) 1164
BpuMI CCSGG 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 1216
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 692, 797
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 798, 1087
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 2 cut(s) 23, 1080
Bse3DI GCAATG 3 cut(s) 416, 581, 768
BseBI CCWGG 3 cut(s) 694, 1102, 1128
BseDI CCNNGG 2 cut(s) 692, 797
BseGI GGATG 3 cut(s) 973, 1096, 1198
BseLI CCNNNNNNNGG 2 cut(s) 798, 1087
BseMI GCAATG 3 cut(s) 416, 581, 768
BseMII CTCAG 4 cut(s) 531, 627, 1178, 1307
BseNI ACTGG 2 cut(s) 23, 1080
BseSI GKGCMC 2 cut(s) 285, 456
BseXI GCAGC 2 cut(s) 196, 760
BseYI CCCAGC 2 cut(s) 764, 915
BshFI GGCC 3 cut(s) 899, 1057, 1258
BsiHKAI GWGCWC 3 cut(s) 225, 456, 924
BsiHKCI CYCGRG 2 cut(s) 284, 798
BsiSI CCGG 3 cut(s) 10, 255, 911
BslFI GGGAC 2 cut(s) 352, 1297
BslI CCNNNNNNNGG 2 cut(s) 798, 1087
BsmAI GTCTC 2 cut(s) 105, 1199
BsmFI GGGAC 2 cut(s) 352, 1297
BsnI GGCC 3 cut(s) 899, 1057, 1258
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 2 cut(s) 284, 798
Bsp1286I GDGCHC 4 cut(s) 225, 285, 456, 924
Bsp143I GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
Bsp1720I GCTNAGC 1 cut(s) 1164
BspACI CCGC 5 cut(s) 115, 261, 421, 871, 1244
BspANI GGCC 3 cut(s) 899, 1057, 1258
BspCNI CTCAG 4 cut(s) 532, 628, 1177, 1306
BspHI TCATGA 1 cut(s) 1032
BspLI GGNNCC 4 cut(s) 259, 687, 1285, 1286
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 5 cut(s) 755, 944, 965, 1118, 1122
BspQI GCTCTTC 2 cut(s) 99, 167
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 3 cut(s) 416, 581, 768
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 2 cut(s) 23, 1080
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 692, 797
BssMI GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
BssNI GRCGYC 1 cut(s) 1216
BssSI CACGAG 1 cut(s) 957
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 3 cut(s) 694, 1102, 1128
Bst4CI ACNGT 5 cut(s) 392, 407, 533, 1150, 1280
Bst6I CTCTTC 3 cut(s) 99, 167, 1325
BstACI GRCGYC 1 cut(s) 1216
BstAPI GCANNNNNTGC 1 cut(s) 418
BstC8I GCNNGC 6 cut(s) 77, 90, 172, 256, 281, 414
BstDEI CTNAG 4 cut(s) 540, 636, 1164, 1293
BstF5I GGATG 3 cut(s) 973, 1096, 1198
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 8 cut(s) 763, 880, 942, 952, 973, 1117, 1126, 1275
BstMAI GTCTC 2 cut(s) 105, 1199
BstMBI GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
BstMWI GCNNNNNNNGC 6 cut(s) 85, 260, 418, 1013, 1022, 1264
BstNI CCWGG 3 cut(s) 694, 1102, 1128
BstNSI RCATGY 1 cut(s) 1267
BstSCI CCNGG 4 cut(s) 692, 910, 1100, 1126
BstSFI CTRYAG 1 cut(s) 669
BstSLI GKGCMC 2 cut(s) 285, 456
BstV1I GCAGC 2 cut(s) 196, 760
BstV2I GAAGAC 1 cut(s) 6
BstX2I RGATCY 4 cut(s) 760, 949, 1114, 1123
BstXI CCANNNNNNTGG 1 cut(s) 1065
BstYI RGATCY 4 cut(s) 760, 949, 1114, 1123
BsuRI GGCC 3 cut(s) 899, 1057, 1258
BtsCI GGATG 3 cut(s) 973, 1096, 1198
BtsIMutI CAGTG 1 cut(s) 1285
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 6 cut(s) 77, 90, 172, 256, 281, 414
CciI TCATGA 1 cut(s) 1032
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 2 cut(s) 1078, 1284
CseI GACGC 2 cut(s) 118, 1205
Csp6I GTAC 2 cut(s) 426, 1253
CviAII CATG 3 cut(s) 754, 1033, 1264
CviQI GTAC 2 cut(s) 426, 1253
DdeI CTNAG 4 cut(s) 540, 636, 1164, 1293
DpnI GATC 8 cut(s) 762, 879, 941, 951, 972, 1116, 1125, 1274
DpnII GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 897, 1256
Eam1104I CTCTTC 3 cut(s) 99, 167, 1325
EarI CTCTTC 3 cut(s) 99, 167, 1325
EciI GGCGGA 2 cut(s) 250, 1259
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 1078, 1284
Eco47III AGCGCT 1 cut(s) 354
Eco88I CYCGRG 2 cut(s) 284, 798
EcoO109I RGGNCCY 1 cut(s) 1284
EcoRII CCWGG 3 cut(s) 692, 1100, 1126
EcoT22I ATGCAT 1 cut(s) 1096
FaeI CATG 3 cut(s) 757, 1036, 1267
FaqI GGGAC 2 cut(s) 352, 1297
FatI CATG 3 cut(s) 753, 1032, 1263
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 2 cut(s) 185, 774
FokI GGATG 3 cut(s) 980, 1103, 1205
Fsp4HI GCNGC 2 cut(s) 185, 774
FspBI CTAG 2 cut(s) 335, 356
GlaI GCGC 1 cut(s) 354
GluI GCNGC 2 cut(s) 185, 774
GsaI CCCAGC 2 cut(s) 768, 919
GsuI CTGGAG 2 cut(s) 1032, 1123
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 3 cut(s) 899, 1057, 1258
HapII CCGG 3 cut(s) 10, 255, 911
HgaI GACGC 2 cut(s) 118, 1205
HhaI GCGC 1 cut(s) 355
Hin1I GRCGYC 1 cut(s) 1216
Hin1II CATG 3 cut(s) 757, 1036, 1267
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 3 cut(s) 663, 863, 1324
HpaII CCGG 3 cut(s) 10, 255, 911
HphI GGTGA 2 cut(s) 47, 385
Hpy166II GTNNAC 3 cut(s) 426, 454, 748
Hpy188I TCNGA 2 cut(s) 662, 1329
Hpy188III TCNNGA 6 cut(s) 108, 133, 635, 887, 1033, 1220
Hpy8I GTNNAC 3 cut(s) 426, 454, 748
HpyAV CCTTC 4 cut(s) 364, 1006, 1091, 1243
HpyCH4III ACNGT 5 cut(s) 392, 407, 533, 1150, 1280
HpyCH4IV ACGT 2 cut(s) 249, 268
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 260, 418, 1013, 1022, 1264
HpyF3I CTNAG 4 cut(s) 540, 636, 1164, 1293
HpySE526I ACGT 2 cut(s) 249, 268
Hsp92I GRCGYC 1 cut(s) 1216
Hsp92II CATG 3 cut(s) 757, 1036, 1267
HspAI GCGC 1 cut(s) 353
KflI GGGWCCC 1 cut(s) 1284
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
LguI GCTCTTC 2 cut(s) 99, 167
LmnI GCTCC 4 cut(s) 263, 685, 919, 1104
Lsp1109I GCAGC 2 cut(s) 196, 760
LweI GCATC 3 cut(s) 1081, 1103, 1183
MaeI CTAG 2 cut(s) 335, 356
MaeII ACGT 2 cut(s) 249, 268
MaeIII GTNAC 1 cut(s) 812
MalI GATC 8 cut(s) 762, 879, 941, 951, 972, 1116, 1125, 1274
MboI GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
MflI RGATCY 4 cut(s) 760, 949, 1114, 1123
MhlI GDGCHC 4 cut(s) 225, 285, 456, 924
MlsI TGGCCA 1 cut(s) 899
MluCI AATT 5 cut(s) 65, 469, 650, 1063, 1185
MluNI TGGCCA 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 64, 122
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 1096
MroNI GCCGGC 1 cut(s) 254
MscI TGGCCA 1 cut(s) 899
MseI TTAA 2 cut(s) 1158, 1363
MslI CAYNNNNRTG 1 cut(s) 421
Msp20I TGGCCA 1 cut(s) 899
MspI CCGG 3 cut(s) 10, 255, 911
MspR9I CCNGG 4 cut(s) 694, 912, 1102, 1128
MvaI CCWGG 3 cut(s) 694, 1102, 1128
MwoI GCNNNNNNNGC 6 cut(s) 85, 260, 418, 1013, 1022, 1264
NaeI GCCGGC 1 cut(s) 256
NciI CCSGG 1 cut(s) 912
NdeII GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 3 cut(s) 757, 1036, 1267
NlaIV GGNNCC 4 cut(s) 259, 687, 1285, 1286
NsiI ATGCAT 1 cut(s) 1096
NspI RCATGY 1 cut(s) 1267
PagI TCATGA 1 cut(s) 1032
PciSI GCTCTTC 2 cut(s) 99, 167
PcsI WCGNNNNNNNCGW 4 cut(s) 126, 198, 207, 246
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 3 cut(s) 663, 863, 1324
PfoI TCCNGGA 2 cut(s) 1100, 1126
PkrI GCNGC 2 cut(s) 186, 775
PpuMI RGGWCCY 1 cut(s) 1284
Psp5II RGGWCCY 1 cut(s) 1284
Psp6I CCWGG 3 cut(s) 692, 1100, 1126
PspFI CCCAGC 2 cut(s) 764, 915
PspGI CCWGG 3 cut(s) 692, 1100, 1126
PspN4I GGNNCC 4 cut(s) 259, 687, 1285, 1286
PspPI GGNCC 2 cut(s) 1078, 1284
PspPPI RGGWCCY 1 cut(s) 1284
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 4 cut(s) 760, 949, 1114, 1123
RsaI GTAC 2 cut(s) 427, 1254
RsaNI GTAC 2 cut(s) 426, 1253
RseI CAYNNNNRTG 1 cut(s) 421
SapI GCTCTTC 2 cut(s) 99, 167
SaqAI TTAA 2 cut(s) 1158, 1363
SatI GCNGC 2 cut(s) 185, 774
Sau3AI GATC 8 cut(s) 760, 877, 939, 949, 970, 1114, 1123, 1272
Sau96I GGNCC 2 cut(s) 1078, 1284
ScrFI CCNGG 4 cut(s) 694, 912, 1102, 1128
SduI GDGCHC 4 cut(s) 225, 285, 456, 924
SfaNI GCATC 3 cut(s) 1081, 1103, 1183
SfcI CTRYAG 1 cut(s) 669
SinI GGWCC 2 cut(s) 1078, 1284
SmiMI CAYNNNNRTG 1 cut(s) 421
Sse9I AATT 5 cut(s) 65, 469, 650, 1063, 1185
SsiI CCGC 5 cut(s) 115, 261, 421, 871, 1244
SspI AATATT 1 cut(s) 318
SspMI CTAG 2 cut(s) 335, 356
StyD4I CCNGG 4 cut(s) 692, 910, 1100, 1126
TaaI ACNGT 5 cut(s) 392, 407, 533, 1150, 1280
TaiI ACGT 2 cut(s) 252, 271
TaqI TCGA 3 cut(s) 192, 201, 1275
TasI AATT 5 cut(s) 65, 469, 650, 1063, 1185
TfiI GAWTC 3 cut(s) 663, 863, 1324
Tru1I TTAA 2 cut(s) 1158, 1363
Tru9I TTAA 2 cut(s) 1158, 1363
TscAI CASTG 1 cut(s) 1285
TseI GCWGC 2 cut(s) 184, 773
TspDTI ATGAA 6 cut(s) 539, 563, 728, 770, 1049, 1155
TspGWI ACGGA 1 cut(s) 1360
TspRI CASTG 1 cut(s) 1285
VneI GTGCAC 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 1078, 1284
XapI RAATTY 1 cut(s) 1185
XceI RCATGY 1 cut(s) 1267
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XspI CTAG 2 cut(s) 335, 356
Zsp2I ATGCAT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.