pycom03g17700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007332
Physical Location & Seq
Forward (+)
18819562 .. 18821913
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g17700.1

Sequence Viewer

Length: 525 bp
ATGTCAGGCAAAGGCCGTACGCTGAAGAACGTCAAGAAGTTCGCTGACTTTCAATACAAAATATTCACAGAAAAATATGGCCAGCAGCTCATTGACATTTTCGAGTTCCCTGTCAAGCTTGTCCTCGCTCCTTTCACTCTTGCTTTCGACATTGCCGGCTCTGCTCAACGTGGCTTTGGCGTTCCCGAGTTGGTTTCCAAGCTATCTTACATGTCAATCTTTGCAGTTGCTACTTTAGGGACTTATGACATAGCATTAGAGATGGGAAAGAAGGTGATATGCCAAAGGAACTGTGGAACCTGCAATGGTTGGCAGGCATTGCGGTGTACCATGTGCAGAGGATCAGGGAGGGTGCAATACCAAGTGAAGAATTACACCCTGAGAAGTGAAGAGAAAGCAACAGCTGAATGTATTGCAGATGCCATTGCTGATAATCGGGCTGAGTTGGTTCACCTTCCATCCGCCATAAATCTTGATGCGCCATTGCCATCTAAAGAGTGCCCAACCGTGATGGCACGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.23

Weight (kDa)

8.97

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AtTam37 PF25112 89 - 170 7.8e-43 AtTam37 zinc finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AciI CCGC 2 cut(s) 322, 462
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 2 cut(s) 19, 328
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 1 cut(s) 53
AleI CACNNNNGTG 1 cut(s) 520
AluBI AGCT 4 cut(s) 88, 118, 202, 404
AluI AGCT 4 cut(s) 88, 118, 202, 404
AlwI GGATC 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 2 cut(s) 13, 79
ApeKI GCWGC 1 cut(s) 85
AspLEI GCGC 1 cut(s) 481
AsuHPI GGTGA 2 cut(s) 286, 443
AvaI CYCGRG 1 cut(s) 185
BaeGI GKGCMC 1 cut(s) 503
BalI TGGCCA 1 cut(s) 81
BbvI GCAGC 1 cut(s) 97
BccI CCATC 4 cut(s) 256, 466, 496, 505
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 1 cut(s) 86
BlsI GCNGC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 298
BmsI GCATC 2 cut(s) 409, 466
Bse118I RCCGGY 1 cut(s) 155
Bse3DI GCAATG 5 cut(s) 150, 310, 317, 423, 482
BseGI GGATG 1 cut(s) 458
BseMI GCAATG 5 cut(s) 150, 310, 317, 423, 482
BseMII CTCAG 2 cut(s) 371, 432
BseSI GKGCMC 1 cut(s) 503
BseXI GCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 355
BshFI GGCC 2 cut(s) 15, 81
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 156
BsiWI CGTACG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 2 cut(s) 15, 81
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 1 cut(s) 341
BspACI CCGC 2 cut(s) 322, 462
BspANI GGCC 2 cut(s) 15, 81
BspCNI CTCAG 2 cut(s) 372, 433
BspLI GGNNCC 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 1 cut(s) 349
BsrDI GCAATG 5 cut(s) 150, 310, 317, 423, 482
BsrFI RCCGGY 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 155
BssMI GATC 1 cut(s) 341
Bst4CI ACNGT 2 cut(s) 293, 508
Bst6I CTCTTC 1 cut(s) 384
BstAPI GCANNNNNTGC 1 cut(s) 319
BstC8I GCNNGC 3 cut(s) 83, 157, 315
BstDEI CTNAG 2 cut(s) 380, 441
BstF5I GGATG 1 cut(s) 458
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 1 cut(s) 344
BstMBI GATC 1 cut(s) 341
BstMWI GCNNNNNNNGC 2 cut(s) 161, 319
BstNSI RCATGY 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 503
BstV1I GCAGC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 15, 81
BtsCI GGATG 1 cut(s) 458
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 3 cut(s) 83, 157, 315
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 1 cut(s) 155
Csp6I GTAC 2 cut(s) 18, 327
CviAII CATG 2 cut(s) 211, 331
CviJI RGCY 9 cut(s) 15, 81, 88, 118, 159, 174, 202, 404, 440
CviKI_1 RGCY 9 cut(s) 15, 81, 88, 118, 159, 174, 202, 404, 440
CviQI GTAC 2 cut(s) 18, 327
DdeI CTNAG 2 cut(s) 380, 441
DpnI GATC 1 cut(s) 343
DpnII GATC 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 185
FaeI CATG 2 cut(s) 214, 334
FaiI YATR 7 cut(s) 78, 212, 246, 251, 280, 332, 467
FaqI GGGAC 1 cut(s) 253
FatI CATG 2 cut(s) 210, 330
Fnu4HI GCNGC 1 cut(s) 86
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 1 cut(s) 86
GlaI GCGC 1 cut(s) 480
GluI GCNGC 1 cut(s) 86
HaeIII GGCC 2 cut(s) 15, 81
HapII CCGG 1 cut(s) 156
HhaI GCGC 1 cut(s) 481
Hin1II CATG 2 cut(s) 214, 334
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HindIII AAGCTT 1 cut(s) 116
HpaII CCGG 1 cut(s) 156
HphI GGTGA 2 cut(s) 286, 443
Hpy166II GTNNAC 2 cut(s) 327, 451
Hpy188III TCNNGA 3 cut(s) 34, 185, 473
Hpy8I GTNNAC 2 cut(s) 327, 451
HpyAV CCTTC 2 cut(s) 265, 464
HpyCH4III ACNGT 2 cut(s) 293, 508
HpyCH4IV ACGT 2 cut(s) 30, 169
HpyCH4V TGCA 5 cut(s) 224, 303, 336, 355, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 319
HpyF3I CTNAG 2 cut(s) 380, 441
HpySE526I ACGT 2 cut(s) 30, 169
Hsp92II CATG 2 cut(s) 214, 334
HspAI GCGC 1 cut(s) 479
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 341
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 7 cut(s) 95, 123, 169, 299, 313, 330, 392
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 2 cut(s) 409, 466
MaeII ACGT 2 cut(s) 30, 169
MalI GATC 1 cut(s) 343
MboI GATC 1 cut(s) 341
MboII GAAGA 3 cut(s) 37, 379, 401
MhlI GDGCHC 1 cut(s) 503
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 1 cut(s) 370
MluNI TGGCCA 1 cut(s) 81
MnlI CCTC 3 cut(s) 134, 332, 342
Mox20I TGGCCA 1 cut(s) 81
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 1 cut(s) 81
MslI CAYNNNNRTG 2 cut(s) 322, 520
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 161, 319
NaeI GCCGGC 1 cut(s) 157
NdeII GATC 1 cut(s) 341
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 2 cut(s) 214, 334
NlaIV GGNNCC 1 cut(s) 298
NspI RCATGY 1 cut(s) 214
OliI CACNNNNGTG 1 cut(s) 520
PciI ACATGT 1 cut(s) 210
PdiI GCCGGC 1 cut(s) 157
Pfl23II CGTACG 1 cut(s) 17
PkrI GCNGC 1 cut(s) 87
PscI ACATGT 1 cut(s) 210
PspLI CGTACG 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 298
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 2 cut(s) 19, 328
RsaNI GTAC 2 cut(s) 18, 327
RseI CAYNNNNRTG 2 cut(s) 322, 520
SatI GCNGC 1 cut(s) 86
Sau3AI GATC 1 cut(s) 341
SduI GDGCHC 1 cut(s) 503
SetI ASST 9 cut(s) 33, 90, 120, 172, 204, 276, 302, 406, 456
SfaNI GCATC 2 cut(s) 409, 466
SmiMI CAYNNNNRTG 2 cut(s) 322, 520
Sse9I AATT 1 cut(s) 370
SsiI CCGC 2 cut(s) 322, 462
SspI AATATT 1 cut(s) 63
TaaI ACNGT 2 cut(s) 293, 508
TaiI ACGT 2 cut(s) 33, 172
TaqI TCGA 2 cut(s) 102, 147
TasI AATT 1 cut(s) 370
TseI GCWGC 1 cut(s) 85
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.