MD03G1229200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007332
Physical Location & Seq
Forward (+)
31472558 .. 31479124
6567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1229200.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGTCAGGCAAAGGCCGTATGCTGAAGAACGTCAAGAAGTTCGCTGACTTTCAATACAAAATCTTCACAGAAAAATATGGCCAGCAGCTCATTGACATTTTCGAGTTCCCTGTCAAGCTTGTCCTCTCTCCTTTCACTCTTGCTTTCGACATTGCCGGCTCTGCTCAACGTGGCTTTGGCGTCCCCGAGTTGGTTTCCAAGCTATCTTACATGTCAATCTTTGCAGTTGCTACTTTAGGGACTTATGACATAGCATTAGAGATGGGAAAGAAGGTGATATGCCAAAGGAACTGTGGAACCTGCAACGGTTGGCAGGCATTGCGGTGTACCATGTGCAGAGGATCAGGGAGGGTGCAATACCAAGTGAAGAATTACACCCTAAGAAGTGGAGAGAAAGCAACAGCTGAATGTGTTGCAGATGCCATTGCTGATAATCGGGCTGAGTTGGTTCACCTTCCATCCTCCATAAATCTTGATGCGCCATTGCCATCTAAAGAGTGCCCAACCTGTGATGTCACGGGTGTGATGGGCTGTACGGAGTGCAAGGACAAATTACAAGTCAGAATCTCTGCAGATGATATCATGGAGCCTCCATGGAAAGCCTACAATGTTTTGAGAAGATGGACTACCCTTATGAGGCTACATATAGTTCACAGCATGAAAGATCCCAGCATTGCTGCATTTTGGCTGTTTACCTTGCCTCAGGTTGTGGGGGGATTCGACTTCGATGATGATGTCAAGAAAAAAATTTGGTGGCAATACAAGGAATCTATGCGGTATGATCAACTTCGTGATGTGGTGGCCAAGCGAAAACCTGGCTGGGAGCAGTTGCAGGAAGCCTTGATCTCTATAGATCCTGCTCGAGCCAAGGAAGATCCTGTTATTGTGAAAAATATCCCTTACTATAAAGCAAAGAAAGCCCTTGAAACGGATGTCATGAAACTTGATCCTCCACCAAGGCCACAAAATTGGGGTGAACTGGACCTTCCGTTGAATGCATCTTCTTGGAGCAAAAACCCTGAAAAATTCTATGAAATGACTGTTCTTCTAAATGCTCAAAGAGAATTAGCTGACAAAATTTTGGATGCACAGTGGGAGACTATATGGCGTCAAGAAAGGTTGAATGAGATGTTGGAGGAGAAGGTACGGCCGTACATGAAGAATATTGACAGTGGGACCCTCTCCCAGCCTATTATATTACAATCACAAGATCAGAATCAGAAGAGGACCCGACAACGGAGATGGTGGTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

48.22

Weight (kDa)

8.9

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AtTam37 PF25112 89 - 199 1.2e-60 AtTam37 zinc finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AciI CCGC 2 cut(s) 322, 775
AclWI GGATC 5 cut(s) 349, 659, 848, 869, 941
AcoI YGGCCR 3 cut(s) 79, 801, 1148
AcsI RAATTY 3 cut(s) 747, 1025, 1077
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 180, 1108
AfaI GTAC 4 cut(s) 328, 535, 1146, 1154
AfiI CCNNNNNNNGG 4 cut(s) 190, 636, 928, 1236
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 4 cut(s) 53, 926, 994, 1123
AjnI CCWGG 1 cut(s) 814
AjuI GAANNNNNNNTTGG 2 cut(s) 1115, 1147
AleI CACNNNNGTG 1 cut(s) 521
AluBI AGCT 5 cut(s) 88, 118, 202, 404, 1070
AluI AGCT 5 cut(s) 88, 118, 202, 404, 1070
Alw26I GTCTC 1 cut(s) 1091
AlwI GGATC 5 cut(s) 349, 659, 848, 869, 941
Ama87I CYCGRG 2 cut(s) 185, 861
AoxI GGCC 5 cut(s) 13, 79, 801, 959, 1148
ApeKI GCWGC 2 cut(s) 85, 677
ApoI RAATTY 3 cut(s) 747, 1025, 1077
ArsI GACNNNNNNTTYG 2 cut(s) 543, 575
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 3 cut(s) 982, 1176, 1227
AsuHPI GGTGA 3 cut(s) 286, 443, 986
AvaI CYCGRG 2 cut(s) 185, 861
AvaII GGWCC 3 cut(s) 982, 1176, 1227
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 503
BaeI ACNNNNGTAYC 2 cut(s) 1136, 1169
BalI TGGCCA 2 cut(s) 81, 803
BarI GAAGNNNNNNTAC 2 cut(s) 610, 642
BbvI GCAGC 2 cut(s) 97, 664
BccI CCATC 6 cut(s) 256, 466, 496, 520, 615, 1236
BceAI ACGGC 2 cut(s) 1135, 1163
BciT130I CCWGG 1 cut(s) 816
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 1 cut(s) 1091
BfmI CTRYAG 2 cut(s) 570, 849
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 86, 678
BlsI GCNGC 2 cut(s) 87, 679
Bme1390I CCNGG 1 cut(s) 816
Bme18I GGWCC 3 cut(s) 982, 1176, 1227
BmeT110I CYCGRG 2 cut(s) 185, 861
BmgT120I GGNCC 3 cut(s) 982, 1176, 1227
BmiI GGNNCC 5 cut(s) 298, 588, 1177, 1178, 1229
BmrFI CCNGG 1 cut(s) 816
BmsI GCATC 4 cut(s) 409, 466, 1007, 1075
BsaBI GATNNNNATC 1 cut(s) 1215
BsaHI GRCGYC 2 cut(s) 180, 1108
BsaJI CCNNGG 3 cut(s) 593, 867, 956
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 636, 928, 1236
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 984
Bse21I CCTNAGG 1 cut(s) 702
Bse3DI GCAATG 5 cut(s) 150, 317, 423, 482, 672
Bse8I GATNNNNATC 1 cut(s) 1215
BseBI CCWGG 1 cut(s) 816
BseDI CCNNGG 3 cut(s) 593, 867, 956
BseGI GGATG 3 cut(s) 458, 937, 1090
BseJI GATNNNNATC 1 cut(s) 1215
BseLI CCNNNNNNNGG 4 cut(s) 190, 636, 928, 1236
BseMI GCAATG 5 cut(s) 150, 317, 423, 482, 672
BseMII CTCAG 2 cut(s) 432, 716
BseNI ACTGG 1 cut(s) 984
BseRI GAGGAG 1 cut(s) 1151
BseSI GKGCMC 1 cut(s) 503
BseX3I CGGCCG 1 cut(s) 1148
BseXI GCAGC 2 cut(s) 97, 664
BseYI CCCAGC 3 cut(s) 668, 819, 1185
BsgI GTGCAG 1 cut(s) 355
Bsh1285I CGRYCG 1 cut(s) 1151
BshFI GGCC 5 cut(s) 15, 81, 803, 961, 1150
BsiEI CGRYCG 1 cut(s) 1151
BsiHKCI CYCGRG 2 cut(s) 185, 861
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 3 cut(s) 167, 253, 1189
BslI CCNNNNNNNGG 4 cut(s) 190, 636, 928, 1236
BsmAI GTCTC 1 cut(s) 1091
BsmFI GGGAC 3 cut(s) 167, 253, 1189
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 5 cut(s) 15, 81, 803, 961, 1150
BsoBI CYCGRG 2 cut(s) 185, 861
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 2 cut(s) 322, 775
BspANI GGCC 5 cut(s) 15, 81, 803, 961, 1150
BspCNI CTCAG 2 cut(s) 433, 715
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 5 cut(s) 298, 588, 1177, 1178, 1229
BspMAI CTGCAG 1 cut(s) 574
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 5 cut(s) 349, 659, 848, 869, 941
BsrDI GCAATG 5 cut(s) 150, 317, 423, 482, 672
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 984
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 3 cut(s) 593, 867, 956
BssMI GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
BssNI GRCGYC 2 cut(s) 180, 1108
BssT1I CCWWGG 3 cut(s) 593, 867, 956
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 5 cut(s) 293, 308, 1042, 1092, 1172
Bst6I CTCTTC 1 cut(s) 1217
BstACI GRCGYC 2 cut(s) 180, 1108
BstAPI GCANNNNNTGC 1 cut(s) 319
BstC8I GCNNGC 3 cut(s) 83, 157, 315
BstDEI CTNAG 3 cut(s) 380, 441, 702
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 3 cut(s) 458, 937, 1090
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 8 cut(s) 344, 667, 784, 846, 856, 877, 949, 1213
BstMAI GTCTC 1 cut(s) 1091
BstMBI GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
BstMCI CGRYCG 1 cut(s) 1151
BstMWI GCNNNNNNNGC 3 cut(s) 161, 319, 917
BstNI CCWGG 1 cut(s) 816
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 814
BstSFI CTRYAG 2 cut(s) 570, 849
BstSLI GKGCMC 1 cut(s) 503
BstV1I GCAGC 2 cut(s) 97, 664
BstX2I RGATCY 3 cut(s) 664, 853, 874
BstXI CCANNNNNNTGG 1 cut(s) 969
BstYI RGATCY 3 cut(s) 664, 853, 874
BstZI CGGCCG 1 cut(s) 1148
Bsu36I CCTNAGG 1 cut(s) 702
BsuRI GGCC 5 cut(s) 15, 81, 803, 961, 1150
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 3 cut(s) 458, 937, 1090
BtsIMutI CAGTG 2 cut(s) 1097, 1177
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 3 cut(s) 83, 157, 315
CciI TCATGA 1 cut(s) 936
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 3 cut(s) 982, 1176, 1227
CseI GACGC 2 cut(s) 169, 1097
Csp6I GTAC 4 cut(s) 327, 534, 1145, 1153
CviAII CATG 7 cut(s) 211, 331, 583, 594, 658, 937, 1156
CviQI GTAC 4 cut(s) 327, 534, 1145, 1153
DdeI CTNAG 3 cut(s) 380, 441, 702
DpnI GATC 8 cut(s) 343, 666, 783, 845, 855, 876, 948, 1212
DpnII GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
EaeI YGGCCR 3 cut(s) 79, 801, 1148
EagI CGGCCG 1 cut(s) 1148
Eam1104I CTCTTC 1 cut(s) 1217
EarI CTCTTC 1 cut(s) 1217
EclXI CGGCCG 1 cut(s) 1148
Eco130I CCWWGG 3 cut(s) 593, 867, 956
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 3 cut(s) 982, 1176, 1227
Eco52I CGGCCG 1 cut(s) 1148
Eco57I CTGAAG 1 cut(s) 44
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 2 cut(s) 185, 861
EcoO109I RGGNCCY 2 cut(s) 1176, 1227
EcoRII CCWGG 1 cut(s) 814
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 3 cut(s) 593, 867, 956
EcoT22I ATGCAT 1 cut(s) 1000
ErhI CCWWGG 3 cut(s) 593, 867, 956
FaeI CATG 7 cut(s) 214, 334, 586, 597, 661, 940, 1159
FaqI GGGAC 3 cut(s) 167, 253, 1189
FatI CATG 7 cut(s) 210, 330, 582, 593, 657, 936, 1155
FbaI TGATCA 1 cut(s) 781
Fnu4HI GCNGC 2 cut(s) 86, 678
FokI GGATG 3 cut(s) 445, 944, 1097
Fsp4HI GCNGC 2 cut(s) 86, 678
GlaI GCGC 1 cut(s) 480
GluI GCNGC 2 cut(s) 86, 678
GsaI CCCAGC 3 cut(s) 672, 823, 1189
HaeIII GGCC 5 cut(s) 15, 81, 803, 961, 1150
HapII CCGG 1 cut(s) 156
HgaI GACGC 2 cut(s) 169, 1097
HhaI GCGC 1 cut(s) 481
Hin1I GRCGYC 2 cut(s) 180, 1108
Hin1II CATG 7 cut(s) 214, 334, 586, 597, 661, 940, 1159
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 4 cut(s) 564, 717, 767, 1216
HpaII CCGG 1 cut(s) 156
HphI GGTGA 3 cut(s) 286, 443, 986
Hpy166II GTNNAC 5 cut(s) 327, 451, 652, 693, 977
Hpy188I TCNGA 3 cut(s) 563, 1215, 1221
Hpy188III TCNNGA 6 cut(s) 34, 473, 739, 791, 937, 1112
Hpy8I GTNNAC 5 cut(s) 327, 451, 652, 693, 977
HpyAV CCTTC 4 cut(s) 265, 464, 995, 1135
HpyCH4III ACNGT 5 cut(s) 293, 308, 1042, 1092, 1172
HpyCH4IV ACGT 2 cut(s) 30, 169
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 319, 917
HpyF3I CTNAG 3 cut(s) 380, 441, 702
HpySE526I ACGT 2 cut(s) 30, 169
Hsp92I GRCGYC 2 cut(s) 180, 1108
Hsp92II CATG 7 cut(s) 214, 334, 586, 597, 661, 940, 1159
HspAI GCGC 1 cut(s) 479
KflI GGGWCCC 1 cut(s) 1176
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
LmnI GCTCC 3 cut(s) 586, 823, 1008
Lsp1109I GCAGC 2 cut(s) 97, 664
LweI GCATC 4 cut(s) 409, 466, 1007, 1075
MaeII ACGT 2 cut(s) 30, 169
MaeIII GTNAC 1 cut(s) 514
MalI GATC 8 cut(s) 343, 666, 783, 845, 855, 876, 948, 1212
MboI GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
MboII GAAGA 9 cut(s) 37, 55, 379, 630, 884, 993, 1037, 1171, 1234
MflI RGATCY 3 cut(s) 664, 853, 874
MhlI GDGCHC 1 cut(s) 503
MlsI TGGCCA 2 cut(s) 81, 803
MluCI AATT 7 cut(s) 370, 551, 747, 967, 1025, 1064, 1077
MluNI TGGCCA 2 cut(s) 81, 803
MmeI TCCRAC 1 cut(s) 1113
Mox20I TGGCCA 2 cut(s) 81, 803
Mph1103I ATGCAT 1 cut(s) 1000
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 2 cut(s) 81, 803
MslI CAYNNNNRTG 2 cut(s) 322, 521
Msp20I TGGCCA 2 cut(s) 81, 803
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 816
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 1 cut(s) 816
MwoI GCNNNNNNNGC 3 cut(s) 161, 319, 917
NaeI GCCGGC 1 cut(s) 157
NcoI CCATGG 1 cut(s) 593
NdeII GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 7 cut(s) 214, 334, 586, 597, 661, 940, 1159
NlaIV GGNNCC 5 cut(s) 298, 588, 1177, 1178, 1229
NmuCI GTSAC 1 cut(s) 514
NsiI ATGCAT 1 cut(s) 1000
NspI RCATGY 1 cut(s) 214
OliI CACNNNNGTG 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 861
PagI TCATGA 1 cut(s) 936
PciI ACATGT 1 cut(s) 210
PctI GAATGC 1 cut(s) 1000
PdiI GCCGGC 1 cut(s) 157
PfeI GAWTC 4 cut(s) 564, 717, 767, 1216
PkrI GCNGC 2 cut(s) 87, 679
PpuMI RGGWCCY 2 cut(s) 1176, 1227
PscI ACATGT 1 cut(s) 210
Psp5II RGGWCCY 2 cut(s) 1176, 1227
Psp6I CCWGG 1 cut(s) 814
PspFI CCCAGC 3 cut(s) 668, 819, 1185
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 5 cut(s) 298, 588, 1177, 1178, 1229
PspPI GGNCC 3 cut(s) 982, 1176, 1227
PspPPI RGGWCCY 2 cut(s) 1176, 1227
PspXI VCTCGAGB 1 cut(s) 861
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 3 cut(s) 664, 853, 874
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 328, 535, 1146, 1154
RsaNI GTAC 4 cut(s) 327, 534, 1145, 1153
RseI CAYNNNNRTG 2 cut(s) 322, 521
SatI GCNGC 2 cut(s) 86, 678
Sau3AI GATC 8 cut(s) 341, 664, 781, 843, 853, 874, 946, 1210
Sau96I GGNCC 3 cut(s) 982, 1176, 1227
ScrFI CCNGG 1 cut(s) 816
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 4 cut(s) 409, 466, 1007, 1075
SfcI CTRYAG 2 cut(s) 570, 849
Sfr274I CTCGAG 1 cut(s) 861
SinI GGWCC 3 cut(s) 982, 1176, 1227
SlaI CTCGAG 1 cut(s) 861
SmiMI CAYNNNNRTG 2 cut(s) 322, 521
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 7 cut(s) 370, 551, 747, 967, 1025, 1064, 1077
SsiI CCGC 2 cut(s) 322, 775
SspI AATATT 1 cut(s) 1165
StyD4I CCNGG 1 cut(s) 814
StyI CCWWGG 3 cut(s) 593, 867, 956
TaaI ACNGT 5 cut(s) 293, 308, 1042, 1092, 1172
TaiI ACGT 2 cut(s) 33, 172
TaqI TCGA 5 cut(s) 102, 147, 720, 726, 862
TasI AATT 7 cut(s) 370, 551, 747, 967, 1025, 1064, 1077
TfiI GAWTC 4 cut(s) 564, 717, 767, 1216
TscAI CASTG 2 cut(s) 1097, 1177
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 2 cut(s) 85, 677
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 4 cut(s) 674, 953, 1047, 1172
TspGWI ACGGA 4 cut(s) 551, 944, 978, 1252
TspRI CASTG 2 cut(s) 1097, 1177
VpaK11BI GGWCC 3 cut(s) 982, 1176, 1227
XapI RAATTY 3 cut(s) 747, 1025, 1077
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
XhoI CTCGAG 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.