AT5G54290

Cytochrome c-type biogenesis ccda-like chloroplastic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012312
Physical Location & Seq
Forward (+)
22051034 .. 22054028
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54290.1

Sequence Viewer

Length: 1065 bp
ATGAATCTGAGTGTGAACCGATGCATCACCGGTGGTTTCGTCGGTGGATTCTCTAGCTGCAGACTCAATCATGAGAAAAGGTGGGTTCGTGCTGGCAAGCACTGTGAGCTTGAGAGGGAGAGAAGTTTAGTGTCTGATGCTGTTTCTTTAGAGAGGTTAGAGAGCAAAAGCATTAAACTTGCGATGCTTGCTAGTGGTTTAGGAGTGGCGAACTTGGTAACTCTTAGCTCAGCAAAAGCCGCAGACTTGAAGATGATTGTCTTGGATCAAGCCACTTCCATTTACATCTTGGCTGAAGGAAGCCTTGGAGATTCGGTCGGGAACTTCTTGTATTCAGCTAACCAACAGGCTAACGAAGCTGTTCAAGATCAACTATCAGCACTCAGTGTAACAAGTTTGGCAGTAATATTTGGGGCAGGCCTTGTGACAAGTCTGTCTCCATGTACACTTAGTGTTTTGCCGTTGACACTTGGTTATATTGGTGCATTTGGGTCTGGCAAAAGCAGAGGTCAGGTTATTGGAGATACAGTTGCTTTCGCACTGGGATTAGCAACTACCTTAGCCCTTCTTGGGATAGTAGCTTCTTTTGCCGGAAAAGCCTATGGACAAATAGGACAAGGATTACCAGTAGCTGCATCCGGTCTGGCTATTGTAATGGGTCTCAATCTCCTTGAGATAATCGAACTTCAGCTTCCTTCTTTCTTTAACAACTTTGATCCTCGTGCTGCAGCAGCAAACTTCCCATCTAGTGTACAAGCATATCTAGCCGGTCTCACATTCGCACTTGCTGCCTCACCTTGTAGTACTCCAGTTCTAGCCACTCTTCTTGGTTACGTGGCTACTTCCAGGGATCCAGTAATTGGTGGAAGCTTACTCTTAACCTATACAACAGGTTACGTCGCTCCACTTATCGTCGCTGCTTCTTTTGCCGGAGCTTTACAGAGCTTACTTTCGTTGCGAAAGGTCTCTGCATGGATCAATCCCATAAGTGGTGCTCTTCTACTTGGAGGAGGTTTATATACTTTCTTGGATAGGCTTTTTCCTGCTGCTACTATGGTCATGTAA

Protein Analysis

354

Amino Acids

36.53

Weight (kDa)

8.25

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DsbD_TM PF02683 131 - 329 6.8e-23 Cytochrome C biogenesis protein transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54290 AT5G54290
fragaria_vesca FvH4_3g20940 FvH4_3g20940 FvH4_3g20940
malus_domestica MD11G1237300.v1.1
prunus_persica Prupe.4G189900_v2.0.a1 Prupe.4G189900_v2.0.a1
pyrus_communis pycom11g20860
rosa_chinensis RchiOBHm_Chr5g0035361
rosa_laevigata RLG00000033620
rosa_multiflora Rmu_co8507677.1_g000001
rosa_roxburghii Rroxscaffold_1G00045040
rosa_rugosa Rorug05G0151400
rosa_samantha Rh5AG244600 Rh5BG244100 Rh5CG275900 Rh5DG253200
rosa_wichuraiana Rw5G022340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AccB7I CCANNNNNTGG 1 cut(s) 860
AciI CCGC 1 cut(s) 240
AclWI GGATC 5 cut(s) 273, 710, 845, 858, 983
AcuI CTGAAG 2 cut(s) 315, 671
AdeI CACNNNGTG 2 cut(s) 386, 452
AfaI GTAC 3 cut(s) 445, 753, 805
AfiI CCNNNNNNNGG 3 cut(s) 570, 860, 989
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 2 cut(s) 250, 365
AjnI CCWGG 1 cut(s) 845
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 4 cut(s) 441, 665, 776, 970
AlwI GGATC 5 cut(s) 273, 710, 845, 858, 983
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 8 cut(s) 57, 632, 725, 728, 731, 788, 917, 1046
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 360
AsuHPI GGTGA 2 cut(s) 19, 786
BamHI GGATCC 1 cut(s) 850
BauI CACGAG 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 997
BbvI GCAGC 8 cut(s) 44, 619, 712, 740, 743, 775, 904, 1033
BccI CCATC 1 cut(s) 751
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 847
BcoDI GTCTC 4 cut(s) 441, 665, 776, 970
BfaI CTAG 5 cut(s) 54, 192, 747, 764, 815
BfmI CTRYAG 2 cut(s) 58, 726
BisI GCNGC 9 cut(s) 58, 240, 633, 726, 729, 732, 789, 918, 1047
BlpI GCTNAGC 1 cut(s) 229
BlsI GCNGC 9 cut(s) 59, 241, 634, 727, 730, 733, 790, 919, 1048
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 1 cut(s) 847
BmiI GGNNCC 1 cut(s) 852
BmrFI CCNGG 1 cut(s) 847
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 5 cut(s) 11, 33, 127, 174, 644
BmuI ACTGGG 1 cut(s) 551
BpmI CTGGAG 1 cut(s) 792
Bpu10I CCTNAGC 1 cut(s) 559
Bpu1102I GCTNAGC 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 131, 692
BsaAI YACGTR 1 cut(s) 835
BsaI GGTCTC 3 cut(s) 665, 776, 970
BsaJI CCNNGG 2 cut(s) 304, 846
BsaWI WCCGGW 2 cut(s) 29, 638
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 513, 543
Bsc4I CCNNNNNNNGG 3 cut(s) 570, 860, 989
Bse118I RCCGGY 2 cut(s) 29, 767
Bse1I ACTGG 4 cut(s) 546, 626, 809, 854
BseBI CCWGG 1 cut(s) 847
BseDI CCNNGG 2 cut(s) 304, 846
BseGI GGATG 1 cut(s) 635
BseLI CCNNNNNNNGG 3 cut(s) 570, 860, 989
BseMII CTCAG 2 cut(s) 243, 397
BseNI ACTGG 4 cut(s) 546, 626, 809, 854
BseRI GAGGAG 1 cut(s) 1023
BseXI GCAGC 8 cut(s) 44, 619, 712, 740, 743, 775, 904, 1033
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 1 cut(s) 420
BshTI ACCGGT 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 997
BsiSI CCGG 5 cut(s) 30, 591, 639, 768, 930
BslI CCNNNNNNNGG 3 cut(s) 570, 860, 989
BsmAI GTCTC 4 cut(s) 441, 665, 776, 970
BsnI GGCC 1 cut(s) 420
Bso31I GGTCTC 3 cut(s) 665, 776, 970
Bsp1286I GDGCHC 1 cut(s) 997
Bsp1407I TGTACA 2 cut(s) 443, 751
Bsp143I GATC 5 cut(s) 265, 367, 715, 850, 975
Bsp1720I GCTNAGC 1 cut(s) 229
BspACI CCGC 1 cut(s) 240
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 242, 396
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 852
BspMAI CTGCAG 2 cut(s) 62, 730
BspPI GGATC 5 cut(s) 273, 710, 845, 858, 983
BspQI GCTCTTC 1 cut(s) 1002
BspTNI GGTCTC 3 cut(s) 665, 776, 970
BsrFI RCCGGY 2 cut(s) 29, 767
BsrGI TGTACA 2 cut(s) 443, 751
BsrI ACTGG 4 cut(s) 546, 626, 809, 854
BssAI RCCGGY 2 cut(s) 29, 767
BssECI CCNNGG 2 cut(s) 304, 846
BssMI GATC 5 cut(s) 265, 367, 715, 850, 975
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 304
Bst2BI CACGAG 1 cut(s) 720
Bst2UI CCWGG 1 cut(s) 847
Bst4CI ACNGT 2 cut(s) 104, 529
Bst6I CTCTTC 2 cut(s) 828, 1002
BstAPI GCANNNNNTGC 1 cut(s) 788
BstAUI TGTACA 2 cut(s) 443, 751
BstBAI YACGTR 1 cut(s) 835
BstC8I GCNNGC 4 cut(s) 94, 98, 189, 418
BstDEI CTNAG 6 cut(s) 8, 224, 229, 383, 449, 559
BstF5I GGATG 1 cut(s) 635
BstKTI GATC 5 cut(s) 268, 370, 718, 853, 978
BstMAI GTCTC 4 cut(s) 441, 665, 776, 970
BstMBI GATC 5 cut(s) 265, 367, 715, 850, 975
BstMCI CGRYCG 1 cut(s) 318
BstNI CCWGG 1 cut(s) 847
BstSCI CCNGG 1 cut(s) 845
BstSFI CTRYAG 2 cut(s) 58, 726
BstV1I GCAGC 8 cut(s) 44, 619, 712, 740, 743, 775, 904, 1033
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
BsuRI GGCC 1 cut(s) 420
BtgZI GCGATG 1 cut(s) 197
BtsCI GGATG 1 cut(s) 635
BtsIMutI CAGTG 3 cut(s) 100, 391, 539
Cac8I GCNNGC 4 cut(s) 94, 98, 189, 418
CaiI CAGNNNCTG 1 cut(s) 632
CciI TCATGA 1 cut(s) 70
Cfr10I RCCGGY 2 cut(s) 29, 767
Csp6I GTAC 3 cut(s) 444, 752, 804
CspAI ACCGGT 1 cut(s) 29
CspCI CAANNNNNGTGG 2 cut(s) 808, 843
CviAII CATG 4 cut(s) 71, 441, 972, 1060
CviQI GTAC 3 cut(s) 444, 752, 804
DdeI CTNAG 6 cut(s) 8, 224, 229, 383, 449, 559
DpnI GATC 5 cut(s) 267, 369, 717, 852, 977
DpnII GATC 5 cut(s) 265, 367, 715, 850, 975
DraIII CACNNNGTG 2 cut(s) 386, 452
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 828, 1002
EarI CTCTTC 2 cut(s) 828, 1002
Eco130I CCWWGG 1 cut(s) 304
Eco147I AGGCCT 1 cut(s) 420
Eco31I GGTCTC 3 cut(s) 665, 776, 970
Eco57I CTGAAG 2 cut(s) 315, 671
EcoRII CCWGG 1 cut(s) 845
EcoT14I CCWWGG 1 cut(s) 304
EcoT22I ATGCAT 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 4 cut(s) 74, 444, 975, 1063
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FatI CATG 4 cut(s) 70, 440, 971, 1059
Fnu4HI GCNGC 9 cut(s) 58, 240, 633, 726, 729, 732, 789, 918, 1047
FokI GGATG 1 cut(s) 622
Fsp4HI GCNGC 9 cut(s) 58, 240, 633, 726, 729, 732, 789, 918, 1047
FspBI CTAG 5 cut(s) 54, 192, 747, 764, 815
GluI GCNGC 9 cut(s) 58, 240, 633, 726, 729, 732, 789, 918, 1047
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 1 cut(s) 420
HapII CCGG 5 cut(s) 30, 591, 639, 768, 930
Hin1II CATG 4 cut(s) 74, 444, 975, 1063
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 1 cut(s) 868
HinfI GANTC 4 cut(s) 4, 48, 63, 311
HpaII CCGG 5 cut(s) 30, 591, 639, 768, 930
HphI GGTGA 2 cut(s) 19, 786
Hpy166II GTNNAC 4 cut(s) 16, 446, 465, 752
Hpy188I TCNGA 2 cut(s) 9, 136
Hpy188III TCNNGA 3 cut(s) 71, 319, 365
Hpy8I GTNNAC 4 cut(s) 16, 446, 465, 752
Hpy99I CGWCG 3 cut(s) 44, 902, 917
HpyAV CCTTC 3 cut(s) 290, 575, 705
HpyCH4III ACNGT 2 cut(s) 104, 529
HpyCH4IV ACGT 2 cut(s) 834, 897
HpyCH4V TGCA 6 cut(s) 24, 60, 485, 635, 728, 971
HpyF3I CTNAG 6 cut(s) 8, 224, 229, 383, 449, 559
HpySE526I ACGT 2 cut(s) 834, 897
Hsp92II CATG 4 cut(s) 74, 444, 975, 1063
Kzo9I GATC 5 cut(s) 265, 367, 715, 850, 975
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 2 cut(s) 907, 932
Lsp1109I GCAGC 8 cut(s) 44, 619, 712, 740, 743, 775, 904, 1033
LweI GCATC 5 cut(s) 11, 33, 127, 174, 644
MaeI CTAG 5 cut(s) 54, 192, 747, 764, 815
MaeII ACGT 2 cut(s) 834, 897
MaeIII GTNAC 5 cut(s) 217, 388, 424, 830, 893
MalI GATC 5 cut(s) 267, 369, 717, 852, 977
MboI GATC 5 cut(s) 265, 367, 715, 850, 975
MboII GAAGA 3 cut(s) 262, 815, 989
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 1 cut(s) 997
MluCI AATT 1 cut(s) 858
MlyI GAGTC 1 cut(s) 57
MnlI CCTC 7 cut(s) 108, 147, 500, 729, 802, 1001, 1004
Mph1103I ATGCAT 1 cut(s) 26
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 174, 705, 878
MspI CCGG 5 cut(s) 30, 591, 639, 768, 930
MspR9I CCNGG 1 cut(s) 847
MvaI CCWGG 1 cut(s) 847
NdeII GATC 5 cut(s) 265, 367, 715, 850, 975
NlaIII CATG 4 cut(s) 74, 444, 975, 1063
NlaIV GGNNCC 1 cut(s) 852
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 26
PagI TCATGA 1 cut(s) 70
PceI AGGCCT 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 1002
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 4, 48, 311
PflMI CCANNNNNTGG 1 cut(s) 860
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 9 cut(s) 59, 241, 634, 727, 730, 733, 790, 919, 1048
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 835
Psp6I CCWGG 1 cut(s) 845
PspGI CCWGG 1 cut(s) 845
PspN4I GGNNCC 1 cut(s) 852
PstI CTGCAG 2 cut(s) 62, 730
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 3 cut(s) 445, 753, 805
RsaNI GTAC 3 cut(s) 444, 752, 804
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 3 cut(s) 174, 705, 878
SatI GCNGC 9 cut(s) 58, 240, 633, 726, 729, 732, 789, 918, 1047
Sau3AI GATC 5 cut(s) 265, 367, 715, 850, 975
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 847
SduI GDGCHC 1 cut(s) 997
SfaNI GCATC 5 cut(s) 11, 33, 127, 174, 644
SfcI CTRYAG 2 cut(s) 58, 726
SgrAI CRCCGGYG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 110, 671
SmoI CTYRAG 2 cut(s) 110, 671
Sse9I AATT 1 cut(s) 858
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 1 cut(s) 240
SspI AATATT 1 cut(s) 408
SspMI CTAG 5 cut(s) 54, 192, 747, 764, 815
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 845
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 104, 529
TaiI ACGT 2 cut(s) 837, 900
TaqI TCGA 1 cut(s) 681
TaqII GACCGA 1 cut(s) 304
TasI AATT 1 cut(s) 858
TatI WGTACW 3 cut(s) 443, 751, 803
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 3 cut(s) 4, 48, 311
Tru1I TTAA 3 cut(s) 174, 705, 878
Tru9I TTAA 3 cut(s) 174, 705, 878
TscAI CASTG 3 cut(s) 107, 391, 546
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 8 cut(s) 57, 632, 725, 728, 731, 788, 917, 1046
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 107, 391, 546
Van91I CCANNNNNTGG 1 cut(s) 860
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 5 cut(s) 54, 192, 747, 764, 815
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.