AT5G55130

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012535
Physical Location & Seq
Reverse (-)
22373168 .. 22376138
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55130.1

Sequence Viewer

Length: 1395 bp
ATGATGTCCAACGGTGGTGATTCGTCGGAAATTGTCCGGGAACTGGAAGAGTTAAAGCTCAAGAAGGCAGAAATAGAGCACCGTATTTCGACCCTCGAAGCTAAGCTCCAGGATACGGCGGCAGTTGAACTGTACGACGCCGTATCCAACGGTGACTCTTATCTGACGGCACCGGAGCTTGAACATGGGTTATCTCCAGATCAGATTTACCGTTATAGTCGTCAACTGTTACTTCCTTCGTTTGCTGTTGAAGGGCAGTCAAATCTTTTGAAGTCTTCGGTATTAGTTATTGGAGCAGGAGGACTAGGTTCACCTGCCTTATTGTATCTTGCAGCATGTGGTGTTGGTCAGTTGGGTATTATCGATCATGATGTTGTAGAGCTCAACAATATGCATAGGCAGATTATACACACAGAAGCATTTATTGGACATCCCAAAGTGAAATCTGCTGCTGCTGCTTGTCGCTCGATAAATTCAACGATCAAAGTTGATGAATATGTGGAAGCTCTTCGCACTTCCAATGCTTTGGAAATCCTCAGCCAATATGATATCATAGTCGATGCGACTGATAATCCTCCAAGCCGTTACATGATCAGTGATTGTTGTGTCTTGTTAGGAAAGCCTTTGGTGTCGGGTGCTGCGCTTGGTATGGAAGGGCAGCTTACGGTTTATAATCATAACGGAGGCCCGTGTTACCGGTGTCTTTTTCCCACTCCTCCACCTACCTCAGCGTGCCAAAGATGTTCTGATAGTGGAGTTCTTGGAGTAGTGCCAGGTGTTATTGGTTGTCTACAAGCGCTAGAGACAATTAAACTCGCAAGCCTGGTGGGAGAACCACTCTCTGAACGGATGCTTCTTTTTGATGCTTTATCAGCAAGGATGCGCATTGTCAAGATCAGAGGCAGGTCATCACAGTGCACCGTGTGTGGAGACAATTCATCTTTCAATAAGCAAACTTTTAAAGATTTTGACTATGAGGACTTCACTCAATTTCCTTTATTTGCGGGCCCATTGAACCTGCTTCCTGCAGAATCAAGAATTAGCAGCAAGGAGTTCAAAGAAATTCTTCAAAAGAAGGAACAACATGTTCTGCTCGATGTTCGACCTTCACATCACTATAAGATTGTCTCTCTCCCGGATTCACTCAACATCCCTCTTGCAAACTTAGAGACTCGGTTAAACGAGCTTACTTCAGCTCTTAAAGAAAAGGGAAATGGCCATGCGAACACTGAATCTTGCACAAATCCTAGTGTCTTCGTTGTATGCAGACGCGGGAATGATTCACAAAGAGCTGTTCAATATCTTCGTGAGTCGGGTTTTGATTCGGCTAAGGATATAATTGGAGGACTGGAAGCTTGGGCAGCCAATGTCAACCCCAACTTCCCCACCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

464

Amino Acids

50.57

Weight (kDa)

5.55

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 72 - 308 1.9e-82 ThiF family
Rhodanese PF00581 350 - 455 4.6e-12 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 672
AarI CACCTGC 1 cut(s) 322
Acc16I TGCGCA 1 cut(s) 884
Acc36I ACCTGC 3 cut(s) 322, 894, 1026
AccB1I GGYRCC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 790
AccII CGCG 1 cut(s) 1272
AciI CCGC 3 cut(s) 119, 1004, 1272
AcoI YGGCCR 1 cut(s) 1216
AcsI RAATTY 2 cut(s) 472, 1062
AcuI CTGAAG 1 cut(s) 1176
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 924
AfaI GTAC 1 cut(s) 134
AfeI AGCGCT 1 cut(s) 798
AfiI CCNNNNNNNGG 2 cut(s) 43, 115
AflIII ACRYGT 1 cut(s) 1084
AgeI ACCGGT 1 cut(s) 696
AjnI CCWGG 3 cut(s) 108, 772, 822
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
Alw21I GWGCWC 3 cut(s) 81, 384, 920
Alw26I GTCTC 4 cut(s) 797, 924, 1132, 1163
Alw44I GTGCAC 1 cut(s) 916
Aor51HI AGCGCT 1 cut(s) 798
AoxI GGCC 3 cut(s) 685, 1006, 1216
ApaI GGGCCC 1 cut(s) 1010
ApaLI GTGCAC 1 cut(s) 916
ApeKI GCWGC 8 cut(s) 332, 449, 452, 455, 638, 658, 1044, 1361
ApoI RAATTY 2 cut(s) 472, 1062
AsiGI ACCGGT 1 cut(s) 696
Asp700I GAANNNNTTC 2 cut(s) 507, 1065
AspLEI GCGC 3 cut(s) 643, 799, 885
AspS9I GGNCC 3 cut(s) 686, 1006, 1007
AsuC2I CCSGG 2 cut(s) 38, 1136
AsuHPI GGTGA 3 cut(s) 29, 164, 303
BaeGI GKGCMC 2 cut(s) 920, 1010
BalI TGGCCA 1 cut(s) 1218
BanI GGYRCC 1 cut(s) 169
BanII GRGCYC 2 cut(s) 384, 1010
BbsI GAAGAC 2 cut(s) 267, 1246
Bbv12I GWGCWC 3 cut(s) 81, 384, 920
BbvCI CCTCAGC 2 cut(s) 536, 727
BbvI GCAGC 8 cut(s) 344, 436, 439, 442, 625, 670, 1056, 1373
BceAI ACGGC 4 cut(s) 125, 132, 183, 567
BciT130I CCWGG 3 cut(s) 110, 774, 824
BciVI GTATCC 2 cut(s) 106, 154
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 2 cut(s) 38, 1136
BcoDI GTCTC 4 cut(s) 797, 924, 1132, 1163
BfaI CTAG 4 cut(s) 305, 800, 1248, 1393
BfmI CTRYAG 1 cut(s) 1026
BfoI RGCGCY 1 cut(s) 800
BfuAI ACCTGC 3 cut(s) 322, 894, 1026
BfuI GTATCC 2 cut(s) 106, 154
BisI GCNGC 9 cut(s) 120, 333, 450, 453, 456, 639, 659, 1045, 1362
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 9 cut(s) 121, 334, 451, 454, 457, 640, 660, 1046, 1363
Bme1390I CCNGG 5 cut(s) 38, 110, 774, 824, 1136
BmgT120I GGNCC 3 cut(s) 686, 1006, 1007
BmiI GGNNCC 2 cut(s) 171, 1008
BmrFI CCNGG 5 cut(s) 38, 110, 774, 824, 1136
BmsI GCATC 4 cut(s) 550, 840, 853, 870
BpiI GAAGAC 2 cut(s) 267, 1246
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 2 cut(s) 92, 180
Bpu10I CCTNAGC 3 cut(s) 536, 727, 1329
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 38, 1136
Bsa29I ATCGAT 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 138
BsaWI WCCGGW 2 cut(s) 172, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 115
Bse118I RCCGGY 1 cut(s) 696
Bse1I ACTGG 2 cut(s) 48, 1353
BseBI CCWGG 3 cut(s) 110, 774, 824
BseCI ATCGAT 1 cut(s) 363
BseGI GGATG 4 cut(s) 430, 855, 885, 1149
BseLI CCNNNNNNNGG 2 cut(s) 43, 115
BseMII CTCAG 2 cut(s) 550, 741
BseNI ACTGG 2 cut(s) 48, 1353
BseRI GAGGAG 1 cut(s) 705
BseSI GKGCMC 2 cut(s) 920, 1010
BseXI GCAGC 8 cut(s) 344, 436, 439, 442, 625, 670, 1056, 1373
Bsh1236I CGCG 1 cut(s) 1272
BshFI GGCC 3 cut(s) 687, 1008, 1218
BshNI GGYRCC 1 cut(s) 169
BshTI ACCGGT 1 cut(s) 696
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 3 cut(s) 81, 384, 920
BsiSI CCGG 4 cut(s) 37, 173, 697, 1136
BslI CCNNNNNNNGG 2 cut(s) 43, 115
BsmAI GTCTC 4 cut(s) 797, 924, 1132, 1163
BsnI GGCC 3 cut(s) 687, 1008, 1218
Bsp120I GGGCCC 1 cut(s) 1006
Bsp1286I GDGCHC 4 cut(s) 81, 384, 920, 1010
Bsp143I GATC 5 cut(s) 199, 364, 480, 591, 894
Bsp1720I GCTNAGC 1 cut(s) 102
BspACI CCGC 3 cut(s) 119, 1004, 1272
BspANI GGCC 3 cut(s) 687, 1008, 1218
BspCNI CTCAG 2 cut(s) 549, 740
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 1 cut(s) 1272
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 171, 1008
BspMAI CTGCAG 1 cut(s) 1030
BspMI ACCTGC 3 cut(s) 322, 894, 1026
BspQI GCTCTTC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 696
BsrI ACTGG 2 cut(s) 48, 1353
BssAI RCCGGY 1 cut(s) 696
BssMI GATC 5 cut(s) 199, 364, 480, 591, 894
BssNI GRCGYC 1 cut(s) 138
Bst2UI CCWGG 3 cut(s) 110, 774, 824
Bst4CI ACNGT 9 cut(s) 14, 83, 132, 152, 212, 228, 667, 915, 922
Bst6I CTCTTC 2 cut(s) 42, 513
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 3 cut(s) 733, 820, 1006
BstDEI CTNAG 5 cut(s) 102, 536, 727, 1165, 1329
BstF5I GGATG 4 cut(s) 430, 855, 885, 1149
BstFNI CGCG 1 cut(s) 1272
BstH2I RGCGCY 1 cut(s) 800
BstHHI GCGC 3 cut(s) 643, 799, 885
BstKTI GATC 5 cut(s) 202, 367, 483, 594, 897
BstMAI GTCTC 4 cut(s) 797, 924, 1132, 1163
BstMBI GATC 5 cut(s) 199, 364, 480, 591, 894
BstMWI GCNNNNNNNGC 3 cut(s) 455, 872, 1361
BstNI CCWGG 3 cut(s) 110, 774, 824
BstNSI RCATGY 2 cut(s) 339, 1088
BstSCI CCNGG 5 cut(s) 36, 108, 772, 822, 1134
BstSFI CTRYAG 1 cut(s) 1026
BstSLI GKGCMC 2 cut(s) 920, 1010
BstUI CGCG 1 cut(s) 1272
BstV1I GCAGC 8 cut(s) 344, 436, 439, 442, 625, 670, 1056, 1373
BstV2I GAAGAC 2 cut(s) 267, 1246
BstXI CCANNNNNNTGG 1 cut(s) 526
Bsu15I ATCGAT 1 cut(s) 363
BsuI GTATCC 2 cut(s) 106, 154
BsuRI GGCC 3 cut(s) 687, 1008, 1218
BsuTUI ATCGAT 1 cut(s) 363
BtsCI GGATG 4 cut(s) 430, 855, 885, 1149
BtsIMutI CAGTG 3 cut(s) 601, 920, 1227
BveI ACCTGC 3 cut(s) 322, 894, 1026
Cac8I GCNNGC 3 cut(s) 733, 820, 1006
CciI TCATGA 1 cut(s) 367
CfoI GCGC 3 cut(s) 643, 799, 885
Cfr10I RCCGGY 1 cut(s) 696
Cfr13I GGNCC 3 cut(s) 686, 1006, 1007
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 2 cut(s) 146, 1278
Csp6I GTAC 1 cut(s) 133
CspAI ACCGGT 1 cut(s) 696
CspCI CAANNNNNGTGG 2 cut(s) 807, 842
CviAII CATG 6 cut(s) 185, 336, 368, 589, 1085, 1220
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 5 cut(s) 102, 536, 727, 1165, 1329
DpnI GATC 5 cut(s) 201, 366, 482, 593, 896
DpnII GATC 5 cut(s) 199, 364, 480, 591, 894
DraI TTTAAA 1 cut(s) 961
DraIII CACNNNGTG 1 cut(s) 924
EaeI YGGCCR 1 cut(s) 1216
Eam1104I CTCTTC 2 cut(s) 42, 513
EarI CTCTTC 2 cut(s) 42, 513
Ecl136II GAGCTC 1 cut(s) 382
Eco24I GRGCYC 2 cut(s) 384, 1010
Eco32I GATATC 1 cut(s) 550
Eco47III AGCGCT 1 cut(s) 798
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 1176
EcoICRI GAGCTC 1 cut(s) 382
EcoRII CCWGG 3 cut(s) 108, 772, 822
EcoRV GATATC 1 cut(s) 550
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 2 cut(s) 384, 1010
FaeI CATG 6 cut(s) 188, 339, 371, 592, 1088, 1223
FalI AAGNNNNNCTT 4 cut(s) 645, 677, 1050, 1082
FatI CATG 6 cut(s) 184, 335, 367, 588, 1084, 1219
FauI CCCGC 2 cut(s) 997, 1265
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 1 cut(s) 790
Fnu4HI GCNGC 9 cut(s) 120, 333, 450, 453, 456, 639, 659, 1045, 1362
FokI GGATG 4 cut(s) 417, 862, 892, 1136
FriOI GRGCYC 2 cut(s) 384, 1010
Fsp4HI GCNGC 9 cut(s) 120, 333, 450, 453, 456, 639, 659, 1045, 1362
FspAI RTGCGCAY 1 cut(s) 884
FspBI CTAG 4 cut(s) 305, 800, 1248, 1393
FspI TGCGCA 1 cut(s) 884
GlaI GCGC 3 cut(s) 642, 798, 884
GluI GCNGC 9 cut(s) 120, 333, 450, 453, 456, 639, 659, 1045, 1362
GsuI CTGGAG 2 cut(s) 92, 180
HaeII RGCGCY 1 cut(s) 800
HaeIII GGCC 3 cut(s) 687, 1008, 1218
HapII CCGG 4 cut(s) 37, 173, 697, 1136
HgaI GACGC 2 cut(s) 146, 1278
HhaI GCGC 3 cut(s) 643, 799, 885
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 6 cut(s) 188, 339, 371, 592, 1088, 1223
Hin6I GCGC 3 cut(s) 641, 797, 883
HinP1I GCGC 3 cut(s) 641, 797, 883
HincII GTYRAC 2 cut(s) 224, 1372
HindII GTYRAC 2 cut(s) 224, 1372
HindIII AAGCTT 1 cut(s) 1353
HinfI GANTC 9 cut(s) 20, 155, 1031, 1139, 1171, 1232, 1280, 1310, 1322
HpaII CCGG 4 cut(s) 37, 173, 697, 1136
HphI GGTGA 3 cut(s) 29, 164, 303
Hpy166II GTNNAC 5 cut(s) 224, 311, 791, 918, 1372
Hpy188I TCNGA 6 cut(s) 28, 165, 204, 748, 844, 899
Hpy188III TCNNGA 6 cut(s) 61, 197, 368, 892, 1035, 1307
Hpy8I GTNNAC 5 cut(s) 224, 311, 791, 918, 1372
Hpy99I CGWCG 2 cut(s) 28, 140
HpyAV CCTTC 6 cut(s) 58, 245, 246, 647, 1069, 1116
HpyCH4III ACNGT 9 cut(s) 14, 83, 132, 152, 212, 228, 667, 915, 922
HpyCH4V TGCA 7 cut(s) 332, 394, 918, 1028, 1160, 1239, 1266
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 872, 1361
HpyF3I CTNAG 5 cut(s) 102, 536, 727, 1165, 1329
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 6 cut(s) 188, 339, 371, 592, 1088, 1223
HspAI GCGC 3 cut(s) 641, 797, 883
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 5 cut(s) 199, 364, 480, 591, 894
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 3 cut(s) 111, 175, 293
Lsp1109I GCAGC 8 cut(s) 344, 436, 439, 442, 625, 670, 1056, 1373
LweI GCATC 4 cut(s) 550, 840, 853, 870
MaeI CTAG 4 cut(s) 305, 800, 1248, 1393
MaeIII GTNAC 4 cut(s) 152, 228, 584, 692
MalI GATC 5 cut(s) 201, 366, 482, 593, 896
MboI GATC 5 cut(s) 199, 364, 480, 591, 894
MboII GAAGA 6 cut(s) 59, 267, 500, 1058, 1246, 1295
MhlI GDGCHC 4 cut(s) 81, 384, 920, 1010
MlsI TGGCCA 1 cut(s) 1218
MluCI AATT 8 cut(s) 30, 472, 807, 934, 989, 1038, 1062, 1338
MluNI TGGCCA 1 cut(s) 1218
MlyI GAGTC 3 cut(s) 149, 1165, 1319
MmeI TCCRAC 3 cut(s) 6, 33, 171
Mox20I TGGCCA 1 cut(s) 1218
Mph1103I ATGCAT 1 cut(s) 396
MroXI GAANNNNTTC 2 cut(s) 507, 1065
MscI TGGCCA 1 cut(s) 1218
MseI TTAA 5 cut(s) 53, 810, 960, 1178, 1200
MslI CAYNNNNRTG 1 cut(s) 913
Msp20I TGGCCA 1 cut(s) 1218
MspI CCGG 4 cut(s) 37, 173, 697, 1136
MspR9I CCNGG 5 cut(s) 38, 110, 774, 824, 1136
MvaI CCWGG 3 cut(s) 110, 774, 824
MvnI CGCG 1 cut(s) 1272
MwoI GCNNNNNNNGC 3 cut(s) 455, 872, 1361
NciI CCSGG 2 cut(s) 38, 1136
NdeII GATC 5 cut(s) 199, 364, 480, 591, 894
NlaIII CATG 6 cut(s) 188, 339, 371, 592, 1088, 1223
NlaIV GGNNCC 2 cut(s) 171, 1008
NmuCI GTSAC 1 cut(s) 152
NsbI TGCGCA 1 cut(s) 884
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 2 cut(s) 339, 1088
PagI TCATGA 1 cut(s) 367
PaqCI CACCTGC 1 cut(s) 322
PciI ACATGT 1 cut(s) 1084
PciSI GCTCTTC 1 cut(s) 513
PdmI GAANNNNTTC 2 cut(s) 507, 1065
PfeI GAWTC 6 cut(s) 20, 1031, 1139, 1232, 1280, 1322
PfoI TCCNGGA 3 cut(s) 36, 108, 1134
PinAI ACCGGT 1 cut(s) 696
PkrI GCNGC 9 cut(s) 121, 334, 451, 454, 457, 640, 660, 1046, 1363
PleI GAGTC 3 cut(s) 149, 1165, 1318
PpsI GAGTC 3 cut(s) 149, 1165, 1318
PscI ACATGT 1 cut(s) 1084
PsiI TTATAA 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 108, 772, 822
PspGI CCWGG 3 cut(s) 108, 772, 822
PspN4I GGNNCC 2 cut(s) 171, 1008
PspOMI GGGCCC 1 cut(s) 1006
PspPI GGNCC 3 cut(s) 686, 1006, 1007
PstI CTGCAG 1 cut(s) 1030
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 913
SacI GAGCTC 1 cut(s) 384
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 5 cut(s) 53, 810, 960, 1178, 1200
SatI GCNGC 9 cut(s) 120, 333, 450, 453, 456, 639, 659, 1045, 1362
Sau3AI GATC 5 cut(s) 199, 364, 480, 591, 894
Sau96I GGNCC 3 cut(s) 686, 1006, 1007
SchI GAGTC 3 cut(s) 149, 1165, 1319
ScrFI CCNGG 5 cut(s) 38, 110, 774, 824, 1136
SduI GDGCHC 4 cut(s) 81, 384, 920, 1010
SfaNI GCATC 4 cut(s) 550, 840, 853, 870
SfcI CTRYAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 1 cut(s) 913
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 8 cut(s) 30, 472, 807, 934, 989, 1038, 1062, 1338
SsiI CCGC 3 cut(s) 119, 1004, 1272
SspMI CTAG 4 cut(s) 305, 800, 1248, 1393
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 5 cut(s) 36, 108, 772, 822, 1134
TaaI ACNGT 9 cut(s) 14, 83, 132, 152, 212, 228, 667, 915, 922
TaqI TCGA 7 cut(s) 89, 96, 363, 467, 558, 1095, 1102
TasI AATT 8 cut(s) 30, 472, 807, 934, 989, 1038, 1062, 1338
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 6 cut(s) 20, 1031, 1139, 1232, 1280, 1322
Tru1I TTAA 5 cut(s) 53, 810, 960, 1178, 1200
Tru9I TTAA 5 cut(s) 53, 810, 960, 1178, 1200
TscAI CASTG 3 cut(s) 601, 920, 1234
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 8 cut(s) 332, 449, 452, 455, 638, 658, 1044, 1361
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 507, 927
TspGWI ACGGA 2 cut(s) 696, 862
TspRI CASTG 3 cut(s) 601, 920, 1234
VneI GTGCAC 1 cut(s) 916
XapI RAATTY 2 cut(s) 472, 1062
XceI RCATGY 2 cut(s) 339, 1088
XmiI GTMKAC 1 cut(s) 790
XmnI GAANNNNTTC 2 cut(s) 507, 1065
XspI CTAG 4 cut(s) 305, 800, 1248, 1393
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.