Rroxscaffold_1G00039530

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012535
Physical Location & Seq
Reverse (-)
57185548 .. 57189600
4053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039530.1

Sequence Viewer

Length: 1395 bp
ATGGCCGCCGCCGACGGTGGAGAAGTCAGTGCAATTCTCCGCGACCTCGAAAGCTTGAAGGCGAGTAAGAGCGAAATCGAGCTCCGAATCTCGGCCCTCGAAGCTCGCCTGCAAGAGATTAACCTTCAGCAGCAGAACGGTGCCGTTTCGAATGGCTCTTGTGCTCCTCTGCCGTCGTCCGTTGATTCCGGCTACGGTCACGATTTGTCGCCTCAGATGATTTACCGCTACAGTCGCCAGCTCTTGCTTCCCTCTTTCGGAGTCCGAGCTCAGTCGAAGCTCCTAAAGTCATCGATTTTGGTGGTTGGAGCTGGAGGGTTGGGCTCGCCGGCTCTGCTATATCTTGCAGCTTGTGGTGTTGGCCACTTGGGGATTATTGATCATGATGTGGTTGAGCTCAACAATATGCACAGGCAGATTATCCACACTGAAAGATTTATTGGTCAGCCCAAAGTGAGATCTGCTGCGGCTGCTTGTCGATCGATTAACTCCACTATCCAGGTTGTGGAATACCAAGAACCATTACGCACATCCAATGCTTTGGAAATCATAAGCAAATATGATATAATAGTGGATGCCACAGACAATGCTCCTAGCCGTTACATGATAAGCGATTGCTGTGTGGTGCTAGGGAAGCCTCTTGTATCTGGTGCTGCACTTGGATTTGAAGGGCAGCTCACGGTGTACAATTACAATGGTGGTCCATGCTATCGATGCCTATTTCCAACTCCTCCACCTACAACAGCATGTCAAAGGTGTTCTGATAGTGGTGTACTAGGTGTCGGTATCATTGGTTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAGGTGAAGTTGGTGAACCACTCTCAGGACGAATGCTTTTACTTGATTCATTGTCGGGACGGATTCGAATTGTTAAGATCCGAGGAAGGTCCTTGCAATGCGTAGCTTGTGGTCAAAATACAACATTCTCGAAGCAGCAATTTCGGGAATTTGATTATGAAAACTTCACTCAGTCTCCACTAACTCCGTCACCTTTGAAGTTAAACCTACTTCAGGCAGACTCTAGAATAAATACTAAAGAGTACAAGGAGAAAATTGATGACGGGGAAGCACATGTTTTGATTGATGTTCGTCCAGAACACCACTTCAAGATCGTTTCTCTGCCCAATTCGTTAAACATTCCACTCCCAAGTTTGGAGGCACGGTTACCTGAAATCTCTCTAGCTTTAAAGGAAAATGAAGAGAATCGAGGTATTAATTCCAAACTGTATGTAGTATGTAGAAGGGGTAATGATTCTCAGAGGGCTGTTCAGAGTCTCCACAAGCAGGGTTTCACCTCAGCCAGAGACATCATTGGGGGACTGGAGGCCTGGGCTCGTGATGTGGATCCAAACTTTCCTACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

464

Amino Acids

50.5

Weight (kDa)

6.76

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 77 - 309 2.8e-79 ThiF family
Rhodanese PF00581 354 - 455 1.8e-14 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 818
Acc36I ACCTGC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 42
AciI CCGC 5 cut(s) 6, 9, 40, 226, 467
AclWI GGATC 3 cut(s) 898, 1367, 1380
AcoI YGGCCR 2 cut(s) 3, 361
AcsI RAATTY 1 cut(s) 974
AcuI CTGAAG 2 cut(s) 110, 1022
AfaI GTAC 3 cut(s) 686, 774, 1070
AfiI CCNNNNNNNGG 7 cut(s) 91, 257, 505, 851, 1039, 1180, 1312
AflIII ACRYGT 1 cut(s) 1099
AgsI TTSAA 4 cut(s) 58, 668, 1024, 1135
AjnI CCWGG 2 cut(s) 498, 1355
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
Alw21I GWGCWC 4 cut(s) 84, 166, 271, 399
Alw26I GTCTC 4 cut(s) 803, 1005, 1307, 1326
AlwI GGATC 3 cut(s) 898, 1367, 1380
AoxI GGCC 4 cut(s) 3, 93, 361, 1353
ApeKI GCWGC 7 cut(s) 130, 347, 464, 470, 653, 673, 961
ApoI RAATTY 1 cut(s) 974
AseI ATTAAT 1 cut(s) 1242
AspS9I GGNCC 3 cut(s) 94, 701, 915
AsuHPI GGTGA 4 cut(s) 842, 851, 1008, 1312
AsuII TTCGAA 2 cut(s) 149, 892
AvaII GGWCC 2 cut(s) 701, 915
BalI TGGCCA 1 cut(s) 363
BamHI GGATCC 1 cut(s) 1372
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 5 cut(s) 84, 271, 326, 399, 1363
BauI CACGAG 1 cut(s) 1362
Bbv12I GWGCWC 4 cut(s) 84, 166, 271, 399
BbvCI CCTCAGC 1 cut(s) 1324
BbvI GCAGC 7 cut(s) 142, 359, 451, 457, 640, 685, 973
BceAI ACGGC 3 cut(s) 128, 157, 582
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 2 cut(s) 500, 1357
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 4 cut(s) 803, 1005, 1307, 1326
BfaI CTAG 6 cut(s) 594, 629, 776, 809, 1050, 1208
BfmI CTRYAG 1 cut(s) 229
BfuAI ACCTGC 1 cut(s) 818
BglII AGATCT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 500, 1357
Bme18I GGWCC 2 cut(s) 701, 915
BmgT120I GGNCC 3 cut(s) 94, 701, 915
BmiI GGNNCC 2 cut(s) 142, 1374
BmrFI CCNGG 2 cut(s) 500, 1357
BmsI GCATC 2 cut(s) 565, 704
BpmI CTGGAG 2 cut(s) 333, 1370
Bpu10I CCTNAGC 1 cut(s) 1324
Bpu14I TTCGAA 2 cut(s) 149, 892
Bsa29I ATCGAT 3 cut(s) 293, 482, 712
BsaBI GATNNNNATC 1 cut(s) 1371
BsaJI CCNNGG 2 cut(s) 907, 1356
BsaXI ACNNNNNCTCC 4 cut(s) 985, 1015, 1175, 1205
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 257, 505, 851, 1039, 1180, 1312
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 1353
Bse3DI GCAATG 1 cut(s) 929
Bse8I GATNNNNATC 1 cut(s) 1371
BseBI CCWGG 2 cut(s) 500, 1357
BseCI ATCGAT 3 cut(s) 293, 482, 712
BseDI CCNNGG 2 cut(s) 907, 1356
BseGI GGATG 2 cut(s) 530, 580
BseJI GATNNNNATC 1 cut(s) 1371
BseLI CCNNNNNNNGG 7 cut(s) 91, 257, 505, 851, 1039, 1180, 1312
BseMI GCAATG 1 cut(s) 929
BseMII CTCAG 6 cut(s) 227, 284, 864, 1010, 1298, 1338
BseNI ACTGG 1 cut(s) 1353
BseRI GAGGAG 2 cut(s) 156, 720
BseXI GCAGC 7 cut(s) 142, 359, 451, 457, 640, 685, 973
BsgI GTGCAG 1 cut(s) 639
Bsh1236I CGCG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 4 cut(s) 5, 95, 363, 1355
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 3 cut(s) 293, 482, 712
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 4 cut(s) 84, 166, 271, 399
BsiSI CCGG 2 cut(s) 189, 329
BslFI GGGAC 2 cut(s) 897, 1359
BslI CCNNNNNNNGG 7 cut(s) 91, 257, 505, 851, 1039, 1180, 1312
BsmAI GTCTC 4 cut(s) 803, 1005, 1307, 1326
BsmFI GGGAC 2 cut(s) 897, 1359
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 4 cut(s) 5, 95, 363, 1355
Bsp119I TTCGAA 2 cut(s) 149, 892
Bsp1286I GDGCHC 6 cut(s) 84, 166, 271, 326, 399, 1363
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
BspACI CCGC 5 cut(s) 6, 9, 40, 226, 467
BspANI GGCC 4 cut(s) 5, 95, 363, 1355
BspCNI CTCAG 6 cut(s) 226, 283, 863, 1009, 1297, 1337
BspDI ATCGAT 3 cut(s) 293, 482, 712
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 142, 1374
BspMI ACCTGC 1 cut(s) 818
BspPI GGATC 3 cut(s) 898, 1367, 1380
BspT104I TTCGAA 2 cut(s) 149, 892
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 929
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1353
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 2 cut(s) 907, 1356
BssMI GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
BssSI CACGAG 1 cut(s) 1362
Bst2BI CACGAG 1 cut(s) 1362
Bst2UI CCWGG 2 cut(s) 500, 1357
Bst4CI ACNGT 7 cut(s) 17, 140, 197, 233, 682, 1191, 1254
Bst6I CTCTTC 1 cut(s) 1221
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 2 cut(s) 149, 892
BstC8I GCNNGC 5 cut(s) 106, 110, 239, 326, 330
BstDEI CTNAG 6 cut(s) 213, 270, 850, 996, 1284, 1324
BstEII GGTNACC 1 cut(s) 1191
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstF5I GGATG 2 cut(s) 530, 580
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 6 cut(s) 382, 461, 482, 906, 1140, 1375
BstMAI GTCTC 4 cut(s) 803, 1005, 1307, 1326
BstMBI GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
BstMCI CGRYCG 1 cut(s) 482
BstMWI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 812
BstNI CCWGG 2 cut(s) 500, 1357
BstNSI RCATGY 2 cut(s) 750, 1103
BstPI GGTNACC 1 cut(s) 1191
BstSCI CCNGG 2 cut(s) 498, 1355
BstSFI CTRYAG 1 cut(s) 229
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 7 cut(s) 142, 359, 451, 457, 640, 685, 973
BstX2I RGATCY 3 cut(s) 458, 903, 1372
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 3 cut(s) 458, 903, 1372
Bsu15I ATCGAT 3 cut(s) 293, 482, 712
BsuRI GGCC 4 cut(s) 5, 95, 363, 1355
BsuTUI ATCGAT 3 cut(s) 293, 482, 712
BtsCI GGATG 2 cut(s) 530, 580
BtsIMutI CAGTG 2 cut(s) 34, 426
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 5 cut(s) 106, 110, 239, 326, 330
CciI TCATGA 1 cut(s) 382
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 94, 701, 915
ClaI ATCGAT 3 cut(s) 293, 482, 712
Csp6I GTAC 3 cut(s) 685, 773, 1069
CviAII CATG 5 cut(s) 383, 604, 705, 747, 1100
CviQI GTAC 3 cut(s) 685, 773, 1069
DdeI CTNAG 6 cut(s) 213, 270, 850, 996, 1284, 1324
DpnI GATC 6 cut(s) 381, 460, 481, 905, 1139, 1374
DpnII GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
DraI TTTAAA 1 cut(s) 1215
EaeI YGGCCR 2 cut(s) 3, 361
Eam1104I CTCTTC 1 cut(s) 1221
EarI CTCTTC 1 cut(s) 1221
Ecl136II GAGCTC 3 cut(s) 82, 269, 397
Eco147I AGGCCT 1 cut(s) 1355
Eco24I GRGCYC 5 cut(s) 84, 271, 326, 399, 1363
Eco47I GGWCC 2 cut(s) 701, 915
Eco53kI GAGCTC 3 cut(s) 82, 269, 397
Eco57I CTGAAG 2 cut(s) 110, 1022
Eco91I GGTNACC 1 cut(s) 1191
EcoICRI GAGCTC 3 cut(s) 82, 269, 397
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoO109I RGGNCCY 1 cut(s) 915
EcoO65I GGTNACC 1 cut(s) 1191
EcoRII CCWGG 2 cut(s) 498, 1355
EcoT38I GRGCYC 5 cut(s) 84, 271, 326, 399, 1363
FaeI CATG 5 cut(s) 386, 607, 708, 750, 1103
FaqI GGGAC 2 cut(s) 897, 1359
FatI CATG 5 cut(s) 382, 603, 704, 746, 1099
FbaI TGATCA 1 cut(s) 379
FokI GGATG 2 cut(s) 517, 587
FriOI GRGCYC 5 cut(s) 84, 271, 326, 399, 1363
FspBI CTAG 6 cut(s) 594, 629, 776, 809, 1050, 1208
GsuI CTGGAG 2 cut(s) 333, 1370
HaeIII GGCC 4 cut(s) 5, 95, 363, 1355
HapII CCGG 2 cut(s) 189, 329
Hin1II CATG 5 cut(s) 386, 607, 708, 750, 1103
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 9 cut(s) 87, 185, 261, 872, 889, 1046, 1231, 1280, 1300
HpaII CCGG 2 cut(s) 189, 329
HphI GGTGA 4 cut(s) 842, 851, 1008, 1312
Hpy166II GTNNAC 3 cut(s) 685, 773, 842
Hpy188I TCNGA 8 cut(s) 86, 216, 260, 266, 763, 908, 1287, 1299
Hpy8I GTNNAC 3 cut(s) 685, 773, 842
Hpy99I CGWCG 2 cut(s) 17, 178
HpyAV CCTTC 5 cut(s) 52, 134, 662, 906, 1263
HpyCH4III ACNGT 7 cut(s) 17, 140, 197, 233, 682, 1191, 1254
HpyCH4V TGCA 7 cut(s) 32, 112, 347, 409, 656, 827, 922
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 812
HpyF3I CTNAG 6 cut(s) 213, 270, 850, 996, 1284, 1324
Hsp92II CATG 5 cut(s) 386, 607, 708, 750, 1103
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
LmnI GCTCC 5 cut(s) 87, 169, 285, 308, 595
Lsp1109I GCAGC 7 cut(s) 142, 359, 451, 457, 640, 685, 973
LweI GCATC 2 cut(s) 565, 704
MaeI CTAG 6 cut(s) 594, 629, 776, 809, 1050, 1208
MaeIII GTNAC 4 cut(s) 197, 599, 1014, 1191
MalI GATC 6 cut(s) 381, 460, 481, 905, 1139, 1374
MboI GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
MboII GAAGA 1 cut(s) 1238
MflI RGATCY 3 cut(s) 458, 903, 1372
MhlI GDGCHC 6 cut(s) 84, 166, 271, 326, 399, 1363
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 8 cut(s) 33, 688, 894, 965, 974, 1080, 1153, 1243
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 3 cut(s) 270, 1040, 1309
MmeI TCCRAC 2 cut(s) 286, 749
Mox20I TGGCCA 1 cut(s) 363
MroNI GCCGGC 1 cut(s) 328
MscI TGGCCA 1 cut(s) 363
MseI TTAA 8 cut(s) 120, 486, 819, 900, 1028, 1160, 1214, 1242
Msp20I TGGCCA 1 cut(s) 363
MspI CCGG 2 cut(s) 189, 329
MspR9I CCNGG 2 cut(s) 500, 1357
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 2 cut(s) 500, 1357
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 812
NaeI GCCGGC 1 cut(s) 330
NdeII GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 5 cut(s) 386, 607, 708, 750, 1103
NlaIV GGNNCC 2 cut(s) 142, 1374
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 2 cut(s) 197, 1014
NspI RCATGY 2 cut(s) 750, 1103
NspV TTCGAA 2 cut(s) 149, 892
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 818
PceI AGGCCT 1 cut(s) 1355
PciI ACATGT 1 cut(s) 1099
PctI GAATGC 1 cut(s) 864
PdiI GCCGGC 1 cut(s) 330
PfeI GAWTC 6 cut(s) 87, 185, 872, 889, 1231, 1280
PflMI CCANNNNNTGG 1 cut(s) 505
Ple19I CGATCG 1 cut(s) 482
PleI GAGTC 3 cut(s) 269, 1040, 1308
PpsI GAGTC 3 cut(s) 269, 1040, 1308
PpuMI RGGWCCY 1 cut(s) 915
PscI ACATGT 1 cut(s) 1099
PshBI ATTAAT 1 cut(s) 1242
Psp124BI GAGCTC 3 cut(s) 84, 271, 399
Psp5II RGGWCCY 1 cut(s) 915
Psp6I CCWGG 2 cut(s) 498, 1355
PspEI GGTNACC 1 cut(s) 1191
PspGI CCWGG 2 cut(s) 498, 1355
PspN4I GGNNCC 2 cut(s) 142, 1374
PspPI GGNCC 3 cut(s) 94, 701, 915
PspPPI RGGWCCY 1 cut(s) 915
PsuI RGATCY 3 cut(s) 458, 903, 1372
PvuI CGATCG 1 cut(s) 482
RsaI GTAC 3 cut(s) 686, 774, 1070
RsaNI GTAC 3 cut(s) 685, 773, 1069
SacI GAGCTC 3 cut(s) 84, 271, 399
SaqAI TTAA 8 cut(s) 120, 486, 819, 900, 1028, 1160, 1214, 1242
Sau3AI GATC 6 cut(s) 379, 458, 479, 903, 1137, 1372
Sau96I GGNCC 3 cut(s) 94, 701, 915
SchI GAGTC 3 cut(s) 270, 1040, 1309
ScrFI CCNGG 2 cut(s) 500, 1357
SduI GDGCHC 6 cut(s) 84, 166, 271, 326, 399, 1363
SfaNI GCATC 2 cut(s) 565, 704
SfcI CTRYAG 1 cut(s) 229
SfuI TTCGAA 2 cut(s) 149, 892
SinI GGWCC 2 cut(s) 701, 915
Sse9I AATT 8 cut(s) 33, 688, 894, 965, 974, 1080, 1153, 1243
SseBI AGGCCT 1 cut(s) 1355
SsiI CCGC 5 cut(s) 6, 9, 40, 226, 467
SspMI CTAG 6 cut(s) 594, 629, 776, 809, 1050, 1208
SstI GAGCTC 3 cut(s) 84, 271, 399
StuI AGGCCT 1 cut(s) 1355
StyD4I CCNGG 2 cut(s) 498, 1355
TaaI ACNGT 7 cut(s) 17, 140, 197, 233, 682, 1191, 1254
TasI AATT 8 cut(s) 33, 688, 894, 965, 974, 1080, 1153, 1243
TatI WGTACW 3 cut(s) 684, 772, 1068
TauI GCSGC 3 cut(s) 8, 11, 470
TfiI GAWTC 6 cut(s) 87, 185, 872, 889, 1231, 1280
Tru1I TTAA 8 cut(s) 120, 486, 819, 900, 1028, 1160, 1214, 1242
Tru9I TTAA 8 cut(s) 120, 486, 819, 900, 1028, 1160, 1214, 1242
TscAI CASTG 2 cut(s) 34, 433
TseFI GTSAC 2 cut(s) 197, 1014
TseI GCWGC 7 cut(s) 130, 347, 464, 470, 653, 673, 961
Tsp45I GTSAC 2 cut(s) 197, 1014
TspDTI ATGAA 3 cut(s) 864, 999, 1239
TspGWI ACGGA 3 cut(s) 169, 901, 1002
TspRI CASTG 2 cut(s) 34, 433
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 701, 915
VspI ATTAAT 1 cut(s) 1242
XagI CCTNNNNNAGG 1 cut(s) 1037
XapI RAATTY 1 cut(s) 974
XbaI TCTAGA 1 cut(s) 1049
XceI RCATGY 2 cut(s) 750, 1103
XspI CTAG 6 cut(s) 594, 629, 776, 809, 1050, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.