FvH4_3g23360

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012535
Physical Location & Seq
Forward (+)
16399042 .. 16403024
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23360.t1

Sequence Viewer

Length: 996 bp
ATGCACAGGCAGATTATCCACACAGAAAGGTTTATTGGTCAGCCAAAAGTGAGATCTGCTGCGGCTGCTTGTCGATCGATCAACTCCACTATCCAGGTTGAGGAATACCAAGAGCCCTTACGCACATCCAATGCTTTGGAAATCATGAGCAAATATGATATAGTAGTAGATGCCACTGACAATGCTCCTAGCCGTTACATGATTAGCGATTGCTGTGTGGTGCTAGGGAAGCCTCTTGTATCTGGTGCTGCACTAGGATTTGAAGGGCAGCTCACAGTGTACAATTATAATGGTGGTCCATGCTATCGATGCCTATTTCCAACTCCTCCGCCTACAACAGCATGTCAAAGGTGTTCTGATAGTGGTGTACTAGGTGTAGTTCCAGGTATCATTGGCTGTCTACAAGCCCTAGAAGCTATTAAGATTGCAGGTGAAGTTGGTGAACCACTCTCAGGACGGATGCTCTTACTTGATTCATTGTCAGGACGGATTCGAATTGTTAAGATCCGAGGAAGGTCCTTGCAATGTGTAGCTTGTGGTGAAAATACAACATTCTTGAAGCAGCAATTTCGGGAATTTGATTATGAAAACTTCACTCAGTCTCCACTAACTCCGGCACCTTTGAAGTTGAACCTACTTCAGGCAGACTCCAGAATAAATACTAAAAAGTACAAGGAGAAAATTGCTAACGGGGAAGCACATGTGTTGATTGATGTTCGTCCAGAACACCACTTCAAGATTGTTTCTCTGCCCAATTCGTTAAACATTCCACTCCCAAGTTTGGAGGCTCGGTTACCTGAAATCTCTCTAGCTTTGAAGGAAAAGGAAGAGAACAAAGGTATTAATTCTGAACTATATGTAGTATGTAGAAGGGGTAATGATTCCCAGAGGGCTGTTCAGAGTCTCCACAAGCTGGGTTTCACCTCTGCCAGAGACATCATTGGGGGACTGGAGGCCTGGGCTCGTGATGTGGATCCAGACTTTCCTACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

332

Amino Acids

36.54

Weight (kDa)

7.96

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 1 - 176 6.1e-53 ThiF family
Rhodanese PF00581 222 - 322 1.8e-14 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AarI CACCTGC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 419
AccB1I GGYRCC 1 cut(s) 616
AccB7I CCANNNNNTGG 1 cut(s) 913
AccI GTMKAC 1 cut(s) 400
AciI CCGC 2 cut(s) 62, 329
AclWI GGATC 3 cut(s) 499, 968, 981
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 623
AfaI GTAC 3 cut(s) 281, 369, 671
AfiI CCNNNNNNNGG 5 cut(s) 100, 452, 640, 781, 913
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 6 cut(s) 263, 559, 625, 631, 736, 817
AjnI CCWGG 3 cut(s) 93, 382, 956
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
AluBI AGCT 5 cut(s) 271, 416, 533, 812, 913
AluI AGCT 5 cut(s) 271, 416, 533, 812, 913
Alw26I GTCTC 3 cut(s) 606, 908, 927
AlwI GGATC 3 cut(s) 499, 968, 981
AoxI GGCC 1 cut(s) 954
ApeKI GCWGC 5 cut(s) 59, 65, 248, 268, 562
ApoI RAATTY 1 cut(s) 575
AseI ATTAAT 1 cut(s) 843
AspS9I GGNCC 2 cut(s) 296, 516
AsuHPI GGTGA 4 cut(s) 443, 452, 551, 913
AsuII TTCGAA 1 cut(s) 493
AvaII GGWCC 2 cut(s) 296, 516
BamHI GGATCC 1 cut(s) 973
BanI GGYRCC 1 cut(s) 616
BanII GRGCYC 2 cut(s) 117, 964
BauI CACGAG 1 cut(s) 963
BbvI GCAGC 5 cut(s) 46, 52, 235, 280, 574
BceAI ACGGC 1 cut(s) 177
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 3 cut(s) 95, 384, 958
BcoDI GTCTC 3 cut(s) 606, 908, 927
BfaI CTAG 6 cut(s) 189, 224, 254, 371, 410, 809
BfuAI ACCTGC 1 cut(s) 419
BglII AGATCT 1 cut(s) 53
BisI GCNGC 6 cut(s) 60, 63, 66, 249, 269, 563
BlsI GCNGC 6 cut(s) 61, 64, 67, 250, 270, 564
Bme1390I CCNGG 3 cut(s) 95, 384, 958
Bme18I GGWCC 2 cut(s) 296, 516
BmgT120I GGNCC 2 cut(s) 296, 516
BmiI GGNNCC 2 cut(s) 618, 975
BmrFI CCNGG 3 cut(s) 95, 384, 958
BmsI GCATC 3 cut(s) 160, 299, 450
BpmI CTGGAG 2 cut(s) 634, 971
Bpu14I TTCGAA 1 cut(s) 493
Bsa29I ATCGAT 2 cut(s) 77, 307
BsaBI GATNNNNATC 1 cut(s) 972
BsaJI CCNNGG 2 cut(s) 508, 957
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 776, 806
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 452, 640, 781, 913
Bse1I ACTGG 1 cut(s) 954
Bse3DI GCAATG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 3 cut(s) 95, 384, 958
BseCI ATCGAT 2 cut(s) 77, 307
BseDI CCNNGG 2 cut(s) 508, 957
BseGI GGATG 2 cut(s) 125, 465
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 5 cut(s) 100, 452, 640, 781, 913
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 2 cut(s) 465, 611
BseNI ACTGG 1 cut(s) 954
BseRI GAGGAG 1 cut(s) 315
BseXI GCAGC 5 cut(s) 46, 52, 235, 280, 574
BseYI CCCAGC 1 cut(s) 913
BsgI GTGCAG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 956
BshNI GGYRCC 1 cut(s) 616
BshVI ATCGAT 2 cut(s) 77, 307
BsiEI CGRYCG 1 cut(s) 77
BsiSI CCGG 1 cut(s) 614
BslFI GGGAC 1 cut(s) 960
BslI CCNNNNNNNGG 5 cut(s) 100, 452, 640, 781, 913
BsmAI GTCTC 3 cut(s) 606, 908, 927
BsmFI GGGAC 1 cut(s) 960
BsnI GGCC 1 cut(s) 956
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 117, 964
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 5 cut(s) 53, 74, 78, 504, 973
BspACI CCGC 2 cut(s) 62, 329
BspANI GGCC 1 cut(s) 956
BspCNI CTCAG 2 cut(s) 464, 610
BspDI ATCGAT 2 cut(s) 77, 307
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 2 cut(s) 618, 975
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 3 cut(s) 499, 968, 981
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 530
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 954
BssECI CCNNGG 2 cut(s) 508, 957
BssMI GATC 5 cut(s) 53, 74, 78, 504, 973
BssSI CACGAG 1 cut(s) 963
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 3 cut(s) 95, 384, 958
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 822
BstAUI TGTACA 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 493
BstDEI CTNAG 2 cut(s) 451, 597
BstEII GGTNACC 1 cut(s) 792
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 2 cut(s) 125, 465
BstKTI GATC 5 cut(s) 56, 77, 81, 507, 976
BstMAI GTCTC 3 cut(s) 606, 908, 927
BstMBI GATC 5 cut(s) 53, 74, 78, 504, 973
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 4 cut(s) 65, 229, 309, 413
BstNI CCWGG 3 cut(s) 95, 384, 958
BstNSI RCATGY 2 cut(s) 345, 704
BstPI GGTNACC 1 cut(s) 792
BstSCI CCNGG 3 cut(s) 93, 382, 956
BstV1I GCAGC 5 cut(s) 46, 52, 235, 280, 574
BstX2I RGATCY 3 cut(s) 53, 504, 973
BstXI CCANNNNNNTGG 1 cut(s) 136
BstYI RGATCY 3 cut(s) 53, 504, 973
Bsu15I ATCGAT 2 cut(s) 77, 307
BsuRI GGCC 1 cut(s) 956
BsuTUI ATCGAT 2 cut(s) 77, 307
BtsCI GGATG 2 cut(s) 125, 465
BtsIMutI CAGTG 2 cut(s) 174, 282
BveI ACCTGC 1 cut(s) 419
CciI TCATGA 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 296, 516
ClaI ATCGAT 2 cut(s) 77, 307
Csp6I GTAC 3 cut(s) 280, 368, 670
CviAII CATG 5 cut(s) 145, 199, 300, 342, 701
CviQI GTAC 3 cut(s) 280, 368, 670
DdeI CTNAG 2 cut(s) 451, 597
DpnI GATC 5 cut(s) 55, 76, 80, 506, 975
DpnII GATC 5 cut(s) 53, 74, 78, 504, 973
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
EciI GGCGGA 1 cut(s) 318
Eco147I AGGCCT 1 cut(s) 956
Eco24I GRGCYC 2 cut(s) 117, 964
Eco47I GGWCC 2 cut(s) 296, 516
Eco57I CTGAAG 1 cut(s) 623
Eco91I GGTNACC 1 cut(s) 792
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 516
EcoO65I GGTNACC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 93, 382, 956
EcoT38I GRGCYC 2 cut(s) 117, 964
FaeI CATG 5 cut(s) 148, 202, 303, 345, 704
FaqI GGGAC 1 cut(s) 960
FatI CATG 5 cut(s) 144, 198, 299, 341, 700
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 6 cut(s) 60, 63, 66, 249, 269, 563
FokI GGATG 2 cut(s) 112, 472
FriOI GRGCYC 2 cut(s) 117, 964
Fsp4HI GCNGC 6 cut(s) 60, 63, 66, 249, 269, 563
FspBI CTAG 6 cut(s) 189, 224, 254, 371, 410, 809
GluI GCNGC 6 cut(s) 60, 63, 66, 249, 269, 563
GsaI CCCAGC 1 cut(s) 917
GsuI CTGGAG 2 cut(s) 634, 971
HaeIII GGCC 1 cut(s) 956
HapII CCGG 1 cut(s) 614
Hin1II CATG 5 cut(s) 148, 202, 303, 345, 704
HinfI GANTC 5 cut(s) 473, 490, 647, 881, 901
HpaII CCGG 1 cut(s) 614
HphI GGTGA 4 cut(s) 443, 452, 551, 913
Hpy166II GTNNAC 4 cut(s) 280, 368, 401, 443
Hpy188I TCNGA 4 cut(s) 358, 509, 850, 900
Hpy8I GTNNAC 4 cut(s) 280, 368, 401, 443
HpyAV CCTTC 4 cut(s) 257, 507, 811, 864
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 4 cut(s) 4, 251, 428, 523
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 229, 309, 413
HpyF3I CTNAG 2 cut(s) 451, 597
Hsp92II CATG 5 cut(s) 148, 202, 303, 345, 704
Kzo9I GATC 5 cut(s) 53, 74, 78, 504, 973
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 5 cut(s) 46, 52, 235, 280, 574
LweI GCATC 3 cut(s) 160, 299, 450
MaeI CTAG 6 cut(s) 189, 224, 254, 371, 410, 809
MaeIII GTNAC 2 cut(s) 194, 792
MalI GATC 5 cut(s) 55, 76, 80, 506, 975
MboI GATC 5 cut(s) 53, 74, 78, 504, 973
MboII GAAGA 1 cut(s) 839
MflI RGATCY 3 cut(s) 53, 504, 973
MhlI GDGCHC 2 cut(s) 117, 964
MluCI AATT 7 cut(s) 283, 495, 566, 575, 681, 754, 844
MlyI GAGTC 2 cut(s) 641, 910
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 8 cut(s) 94, 243, 336, 503, 778, 882, 934, 946
MseI TTAA 4 cut(s) 420, 501, 761, 843
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 95, 384, 958
MvaI CCWGG 3 cut(s) 95, 384, 958
MwoI GCNNNNNNNGC 4 cut(s) 65, 229, 309, 413
NdeII GATC 5 cut(s) 53, 74, 78, 504, 973
NlaIII CATG 5 cut(s) 148, 202, 303, 345, 704
NlaIV GGNNCC 2 cut(s) 618, 975
NspI RCATGY 2 cut(s) 345, 704
NspV TTCGAA 1 cut(s) 493
PagI TCATGA 1 cut(s) 144
PaqCI CACCTGC 1 cut(s) 419
PceI AGGCCT 1 cut(s) 956
PciI ACATGT 1 cut(s) 700
PfeI GAWTC 3 cut(s) 473, 490, 881
PflMI CCANNNNNTGG 1 cut(s) 913
PkrI GCNGC 6 cut(s) 61, 64, 67, 250, 270, 564
Ple19I CGATCG 1 cut(s) 77
PleI GAGTC 2 cut(s) 641, 909
PpsI GAGTC 2 cut(s) 641, 909
PpuMI RGGWCCY 1 cut(s) 516
PscI ACATGT 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 843
PsiI TTATAA 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 516
Psp6I CCWGG 3 cut(s) 93, 382, 956
PspEI GGTNACC 1 cut(s) 792
PspFI CCCAGC 1 cut(s) 913
PspGI CCWGG 3 cut(s) 93, 382, 956
PspN4I GGNNCC 2 cut(s) 618, 975
PspPI GGNCC 2 cut(s) 296, 516
PspPPI RGGWCCY 1 cut(s) 516
PsuI RGATCY 3 cut(s) 53, 504, 973
PvuI CGATCG 1 cut(s) 77
RsaI GTAC 3 cut(s) 281, 369, 671
RsaNI GTAC 3 cut(s) 280, 368, 670
SaqAI TTAA 4 cut(s) 420, 501, 761, 843
SatI GCNGC 6 cut(s) 60, 63, 66, 249, 269, 563
Sau3AI GATC 5 cut(s) 53, 74, 78, 504, 973
Sau96I GGNCC 2 cut(s) 296, 516
SchI GAGTC 2 cut(s) 641, 910
ScrFI CCNGG 3 cut(s) 95, 384, 958
SduI GDGCHC 2 cut(s) 117, 964
SfaNI GCATC 3 cut(s) 160, 299, 450
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 2 cut(s) 296, 516
Sse9I AATT 7 cut(s) 283, 495, 566, 575, 681, 754, 844
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 2 cut(s) 62, 329
SspMI CTAG 6 cut(s) 189, 224, 254, 371, 410, 809
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 3 cut(s) 93, 382, 956
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 4 cut(s) 73, 77, 307, 493
TasI AATT 7 cut(s) 283, 495, 566, 575, 681, 754, 844
TatI WGTACW 3 cut(s) 279, 367, 669
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 3 cut(s) 473, 490, 881
Tru1I TTAA 4 cut(s) 420, 501, 761, 843
Tru9I TTAA 4 cut(s) 420, 501, 761, 843
TscAI CASTG 2 cut(s) 181, 282
TseI GCWGC 5 cut(s) 59, 65, 248, 268, 562
TspDTI ATGAA 2 cut(s) 465, 600
TspGWI ACGGA 2 cut(s) 472, 502
TspRI CASTG 2 cut(s) 181, 282
Van91I CCANNNNNTGG 1 cut(s) 913
VpaK11BI GGWCC 2 cut(s) 296, 516
VspI ATTAAT 1 cut(s) 843
XagI CCTNNNNNAGG 1 cut(s) 638
XapI RAATTY 1 cut(s) 575
XceI RCATGY 2 cut(s) 345, 704
XmiI GTMKAC 1 cut(s) 400
XspI CTAG 6 cut(s) 189, 224, 254, 371, 410, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.