pycom11g18670

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012535
Physical Location & Seq
Reverse (-)
20843373 .. 20847935
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g18670.9

Sequence Viewer

Length: 1560 bp
ATGAGTGAAATGACAAGATTGCCCCTCGCAAGTGAAACATCGACAAAAGCCGCAAAGAGGCGCGCGCTGCGACCGTCTCTTCCATACCTCAAAAGCTCCCTGCTCGGCTCAGCTTTTACTCTCTGGGACTGTTACTGCGACTGCGACTCCATGGATCACGGCGGCGGCGAGGCCTCTGCGATTCTTCGCCAGCTCGAAGACTTGAAGGCCAGTAAGAGCGAAATAGAGCGTCAAATCTCAGCCCTCGAAGCTCGGTTCCAAGAGATTGCCCTTCAGCAGCCCAACGGCAGCGTTTCCAATGGCTCTTGTCCTCCCACTATCCCGTCCGTTGGTTCGGGCTACGGCCACGATTTGTCGCCTCAGATGATTTACCGCTACAGTCGCCACCTCTTGCTCCCTTCTTTCGGAGTCCAAGGGCAGTCAAAGCTCTTAAAGTCATCGATTTTGGTGGTCGGAGCCGGAGGATTAGGCTCCCCTGTTCTGCTGTATCTTGCAGCCTCTGGTGTTGGCCGATTGGGGGTCGTTGATCATGATGTGGTTGAGCTCAATAATATGCACAGACAGATTATCCACACTGAAAGTTTTATTGGTCAGCCAAAAGTGAAATCTGCTGCAGCTGCTTGTCATTCGATTAACTCCACTATTCAGGTTGTGGAGTACCAAGAACCACTACGTACATCCAATGCTTTGGAAATTATGAGCAAATATGATATAATAGTAGATGCAACAGATAATGCTCCTAGTCGTTACATGATTAACGATTGTTGTGTGGTATTAGGGAAGCCTCTTGTATCTGGTGCTGCGGTGGGATTAGAAGGGCAGCTCACGGTTTTCAATTATAATGGTGGTCCATGCTATCGATGCCTATTTCCAACTCCACCACCTACAACTGCATGTCAAAGATGTTCTGACGCTGGTGTCCTAGGAGTTGTTCCAGGTGTCATTGGCTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAAGTGCAGTTGGAGAACCACTCTCGGAACGCATGCTTTTATTTGATGCATTGTCAGGACGGATACGTAATGTTAAGATTCGAGGACAGTCACCGCAGTGCGTAGTGTGCGGTGTGAAAGCACCATTCTCTAAGCAGCAATTTCAAGAATTTGATTATGAAAACTTCACTCATTCTCCACTGACTCCGTTACCTTTGAAGTTGAACTTACTTCAGCCAGACTCCAGAATAAACAGTAAAGAGTACAAGGAGAAACTAGTTAGTGGCGAGGCACATGTTTTGGTCGACGTTCGTCCAGAACATCACTTCAAGATTGTTTCTCTGCCGAATTCCTTAAACATCCCGCTCTCACGATTGGAGGCTCGGTTGCCGGAAATAACTTCAGCCTTAAAGGAAAAGGAAGACCAGAGGGGTACTGATTCTGGTTCAGGTGGGCAAGTATATGTAATATGTAGAAGGGGTAACGATTCTCAAAGGGCTGTTCAGTATCTCCAGAAGATGGGTTTCACATCGGCCAAAGACATCATCGGTGGACTGGAGGGCTGGGCGCATGAGGTCGATCCAAACGTCCCTACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

520

Amino Acids

56.26

Weight (kDa)

7.14

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AasI GACNNNNNNGTC 1 cut(s) 917
AccB7I CCANNNNNTGG 1 cut(s) 1477
AccBSI CCGCTC 1 cut(s) 1324
AccI GTMKAC 1 cut(s) 1263
AccII CGCG 2 cut(s) 63, 65
AciI CCGC 8 cut(s) 51, 162, 165, 373, 803, 1073, 1089, 1322
AclWI GGATC 2 cut(s) 162, 1532
AcoI YGGCCR 3 cut(s) 343, 508, 1491
AcsI RAATTY 2 cut(s) 1127, 1306
AcuI CTGAAG 3 cut(s) 257, 1175, 1344
AfaI GTAC 4 cut(s) 659, 676, 1223, 1393
AfiI CCNNNNNNNGG 5 cut(s) 57, 329, 404, 517, 1477
AflIII ACRYGT 1 cut(s) 1252
AgsI TTSAA 6 cut(s) 205, 835, 1124, 1177, 1183, 1288
AhlI ACTAGT 1 cut(s) 1234
AjnI CCWGG 1 cut(s) 934
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AleI CACNNNNGTG 1 cut(s) 1075
AluBI AGCT 9 cut(s) 96, 113, 193, 251, 427, 544, 617, 823, 968
AluI AGCT 9 cut(s) 96, 113, 193, 251, 427, 544, 617, 823, 968
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 2 cut(s) 81, 956
AlwI GGATC 2 cut(s) 162, 1532
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 5 cut(s) 171, 207, 343, 508, 1491
ApoI RAATTY 2 cut(s) 1127, 1306
AspA2I CCTAGG 1 cut(s) 922
AspLEI GCGC 4 cut(s) 63, 65, 67, 1528
AspS9I GGNCC 1 cut(s) 848
AsuHPI GGTGA 1 cut(s) 1062
AvaII GGWCC 1 cut(s) 848
AvrII CCTAGG 1 cut(s) 922
BanII GRGCYC 1 cut(s) 546
BbsI GAAGAC 2 cut(s) 204, 1386
Bbv12I GWGCWC 1 cut(s) 546
BccI CCATC 1 cut(s) 1471
BceAI ACGGC 3 cut(s) 175, 301, 358
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 936
BciVI GTATCC 1 cut(s) 1035
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 2 cut(s) 81, 956
BcuI ACTAGT 1 cut(s) 1234
BfaI CTAG 5 cut(s) 741, 923, 962, 1235, 1558
BfmI CTRYAG 2 cut(s) 376, 612
BfuI GTATCC 1 cut(s) 1035
BlnI CCTAGG 1 cut(s) 922
BlpI GCTNAGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 1 cut(s) 848
BmgT120I GGNCC 1 cut(s) 848
BmiI GGNNCC 3 cut(s) 257, 457, 472
BmrFI CCNGG 1 cut(s) 936
BmsI GCATC 3 cut(s) 712, 851, 1015
BoxI GACNNNNGTC 1 cut(s) 1269
BpiI GAAGAC 2 cut(s) 204, 1386
BplI GAGNNNNNCTC 2 cut(s) 984, 1016
BpmI CTGGAG 3 cut(s) 1186, 1454, 1535
Bpu1102I GCTNAGC 1 cut(s) 109
Bsa29I ATCGAT 2 cut(s) 440, 859
BsaAI YACGTR 2 cut(s) 674, 1046
BsaJI CCNNGG 3 cut(s) 150, 412, 922
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 1138, 1168, 1328, 1358
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 329, 404, 517, 1477
Bse1I ACTGG 2 cut(s) 210, 1518
BseBI CCWGG 1 cut(s) 936
BseCI ATCGAT 2 cut(s) 440, 859
BseDI CCNNGG 3 cut(s) 150, 412, 922
BseGI GGATG 2 cut(s) 677, 1317
BseLI CCNNNNNNNGG 5 cut(s) 57, 329, 404, 517, 1477
BseMII CTCAG 3 cut(s) 123, 252, 374
BseNI ACTGG 2 cut(s) 210, 1518
BsePI GCGCGC 2 cut(s) 61, 63
BseYI CCCAGC 1 cut(s) 1521
BsgI GTGCAG 1 cut(s) 1005
Bsh1236I CGCG 2 cut(s) 63, 65
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 5 cut(s) 173, 209, 345, 510, 1493
BshVI ATCGAT 2 cut(s) 440, 859
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 2 cut(s) 459, 1349
BslFI GGGAC 2 cut(s) 140, 1532
BslI CCNNNNNNNGG 5 cut(s) 57, 329, 404, 517, 1477
BsmAI GTCTC 2 cut(s) 81, 956
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 2 cut(s) 140, 1532
BsnI GGCC 5 cut(s) 173, 209, 345, 510, 1493
Bsp1286I GDGCHC 1 cut(s) 546
Bsp143I GATC 3 cut(s) 154, 526, 1537
Bsp1720I GCTNAGC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 8 cut(s) 51, 162, 165, 373, 803, 1073, 1089, 1322
BspANI GGCC 5 cut(s) 173, 209, 345, 510, 1493
BspCNI CTCAG 3 cut(s) 122, 251, 373
BspDI ATCGAT 2 cut(s) 440, 859
BspFNI CGCG 2 cut(s) 63, 65
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 257, 457, 472
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 2 cut(s) 162, 1532
BsrBI CCGCTC 1 cut(s) 1324
BsrI ACTGG 2 cut(s) 210, 1518
BssECI CCNNGG 3 cut(s) 150, 412, 922
BssHII GCGCGC 2 cut(s) 61, 63
BssMI GATC 3 cut(s) 154, 526, 1537
BssT1I CCWWGG 3 cut(s) 150, 412, 922
Bst2UI CCWGG 1 cut(s) 936
Bst4CI ACNGT 6 cut(s) 75, 131, 380, 829, 1068, 1214
Bst6I CTCTTC 1 cut(s) 84
BstBAI YACGTR 2 cut(s) 674, 1046
BstC8I GCNNGC 4 cut(s) 63, 65, 191, 1013
BstDEI CTNAG 4 cut(s) 109, 238, 360, 1110
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 677, 1317
BstFNI CGCG 2 cut(s) 63, 65
BstHHI GCGC 4 cut(s) 63, 65, 67, 1528
BstKTI GATC 3 cut(s) 157, 529, 1540
BstMAI GTCTC 2 cut(s) 81, 956
BstMBI GATC 3 cut(s) 154, 526, 1537
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 8 cut(s) 67, 248, 381, 424, 617, 861, 965, 1086
BstNI CCWGG 1 cut(s) 936
BstNSI RCATGY 3 cut(s) 897, 1015, 1256
BstPAI GACNNNNGTC 1 cut(s) 1269
BstSCI CCNGG 1 cut(s) 934
BstSFI CTRYAG 2 cut(s) 376, 612
BstSNI TACGTA 2 cut(s) 674, 1046
BstUI CGCG 2 cut(s) 63, 65
BstV2I GAAGAC 2 cut(s) 204, 1386
BstXI CCANNNNNNTGG 1 cut(s) 688
Bsu15I ATCGAT 2 cut(s) 440, 859
BsuI GTATCC 1 cut(s) 1035
BsuRI GGCC 5 cut(s) 173, 209, 345, 510, 1493
BsuTUI ATCGAT 2 cut(s) 440, 859
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 677, 1317
BtsI GCAGTG 1 cut(s) 1082
BtsIMutI CAGTG 3 cut(s) 573, 1082, 1157
Cac8I GCNNGC 4 cut(s) 63, 65, 191, 1013
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 529
CfoI GCGC 4 cut(s) 63, 65, 67, 1528
Cfr13I GGNCC 1 cut(s) 848
ClaI ATCGAT 2 cut(s) 440, 859
CseI GACGC 2 cut(s) 218, 920
Csp6I GTAC 4 cut(s) 658, 675, 1222, 1392
CviAII CATG 8 cut(s) 151, 530, 751, 852, 894, 1012, 1253, 1529
CviQI GTAC 4 cut(s) 658, 675, 1222, 1392
DdeI CTNAG 4 cut(s) 109, 238, 360, 1110
DpnI GATC 3 cut(s) 156, 528, 1539
DpnII GATC 3 cut(s) 154, 526, 1537
DrdI GACNNNNNNGTC 1 cut(s) 917
DseDI GACNNNNNNGTC 1 cut(s) 917
EaeI YGGCCR 3 cut(s) 343, 508, 1491
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 544
Eco105I TACGTA 2 cut(s) 674, 1046
Eco130I CCWWGG 3 cut(s) 150, 412, 922
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 848
Eco53kI GAGCTC 1 cut(s) 544
Eco57I CTGAAG 3 cut(s) 257, 1175, 1344
EcoICRI GAGCTC 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 1306
EcoRII CCWGG 1 cut(s) 934
EcoT14I CCWWGG 3 cut(s) 150, 412, 922
EcoT22I ATGCAT 1 cut(s) 1030
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 3 cut(s) 150, 412, 922
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 8 cut(s) 154, 533, 754, 855, 897, 1015, 1256, 1532
FalI AAGNNNNNCTT 2 cut(s) 1169, 1201
FaqI GGGAC 2 cut(s) 140, 1532
FatI CATG 8 cut(s) 150, 529, 750, 851, 893, 1011, 1252, 1528
FauI CCCGC 1 cut(s) 1329
FbaI TGATCA 1 cut(s) 526
FblI GTMKAC 1 cut(s) 1263
FokI GGATG 2 cut(s) 664, 1304
FriOI GRGCYC 1 cut(s) 546
FspBI CTAG 5 cut(s) 741, 923, 962, 1235, 1558
GlaI GCGC 4 cut(s) 62, 64, 66, 1527
GsaI CCCAGC 1 cut(s) 1525
GsuI CTGGAG 3 cut(s) 1186, 1454, 1535
HaeIII GGCC 5 cut(s) 173, 209, 345, 510, 1493
HapII CCGG 2 cut(s) 459, 1349
HgaI GACGC 2 cut(s) 218, 920
HhaI GCGC 4 cut(s) 63, 65, 67, 1528
Hin1II CATG 8 cut(s) 154, 533, 754, 855, 897, 1015, 1256, 1532
Hin6I GCGC 4 cut(s) 61, 63, 65, 1526
HinP1I GCGC 4 cut(s) 61, 63, 65, 1526
HincII GTYRAC 1 cut(s) 1264
HindII GTYRAC 1 cut(s) 1264
HinfI GANTC 8 cut(s) 146, 181, 408, 1057, 1162, 1199, 1397, 1445
HpaII CCGG 2 cut(s) 459, 1349
HphI GGTGA 1 cut(s) 1062
Hpy166II GTNNAC 2 cut(s) 1264, 1511
Hpy188I TCNGA 5 cut(s) 363, 407, 455, 910, 1006
Hpy188III TCNNGA 8 cut(s) 530, 1035, 1124, 1203, 1274, 1288, 1329, 1471
Hpy8I GTNNAC 2 cut(s) 1264, 1511
Hpy99I CGWCG 1 cut(s) 1268
HpyAV CCTTC 5 cut(s) 199, 281, 408, 809, 1428
HpyCH4III ACNGT 6 cut(s) 75, 131, 380, 829, 1068, 1214
HpyCH4IV ACGT 4 cut(s) 673, 1045, 1266, 1545
HpyCH4V TGCA 8 cut(s) 494, 556, 614, 725, 893, 980, 986, 1028
HpyF10VI GCNNNNNNNGC 8 cut(s) 67, 248, 381, 424, 617, 861, 965, 1086
HpyF3I CTNAG 4 cut(s) 109, 238, 360, 1110
HpySE526I ACGT 4 cut(s) 673, 1045, 1266, 1545
Hsp92II CATG 8 cut(s) 154, 533, 754, 855, 897, 1015, 1256, 1532
HspAI GCGC 4 cut(s) 61, 63, 65, 1526
Ksp22I TGATCA 1 cut(s) 526
Kzo9I GATC 3 cut(s) 154, 526, 1537
LmnI GCTCC 5 cut(s) 101, 399, 455, 476, 742
LweI GCATC 3 cut(s) 712, 851, 1015
MaeI CTAG 5 cut(s) 741, 923, 962, 1235, 1558
MaeII ACGT 4 cut(s) 673, 1045, 1266, 1545
MaeIII GTNAC 5 cut(s) 131, 746, 1068, 1167, 1439
MalI GATC 3 cut(s) 156, 528, 1539
MbiI CCGCTC 1 cut(s) 1324
MboI GATC 3 cut(s) 154, 526, 1537
MboII GAAGA 5 cut(s) 71, 176, 209, 1391, 1486
MhlI GDGCHC 1 cut(s) 546
MluCI AATT 5 cut(s) 693, 835, 1118, 1127, 1306
MlyI GAGTC 4 cut(s) 140, 417, 1156, 1193
MmeI TCCRAC 3 cut(s) 433, 896, 970
Mph1103I ATGCAT 1 cut(s) 1030
MseI TTAA 7 cut(s) 431, 633, 756, 972, 1053, 1313, 1367
MslI CAYNNNNRTG 1 cut(s) 1075
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 2 cut(s) 459, 1349
MspR9I CCNGG 1 cut(s) 936
MvaI CCWGG 1 cut(s) 936
MvnI CGCG 2 cut(s) 63, 65
MwoI GCNNNNNNNGC 8 cut(s) 67, 248, 381, 424, 617, 861, 965, 1086
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 154, 526, 1537
NlaIII CATG 8 cut(s) 154, 533, 754, 855, 897, 1015, 1256, 1532
NlaIV GGNNCC 3 cut(s) 257, 457, 472
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 1068
NsiI ATGCAT 1 cut(s) 1030
NspI RCATGY 3 cut(s) 897, 1015, 1256
OliI CACNNNNGTG 1 cut(s) 1075
PaeI GCATGC 1 cut(s) 1015
PagI TCATGA 1 cut(s) 529
PauI GCGCGC 2 cut(s) 61, 63
PceI AGGCCT 1 cut(s) 173
PciI ACATGT 1 cut(s) 1252
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 1542
PfeI GAWTC 4 cut(s) 181, 1057, 1397, 1445
PflMI CCANNNNNTGG 1 cut(s) 1477
PleI GAGTC 4 cut(s) 140, 416, 1156, 1193
PpsI GAGTC 4 cut(s) 140, 416, 1156, 1193
Ppu21I YACGTR 2 cut(s) 674, 1046
PscI ACATGT 1 cut(s) 1252
PshAI GACNNNNGTC 1 cut(s) 1269
PsiI TTATAA 1 cut(s) 840
Psp124BI GAGCTC 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 934
PspFI CCCAGC 1 cut(s) 1521
PspGI CCWGG 1 cut(s) 934
PspN4I GGNNCC 3 cut(s) 257, 457, 472
PspPI GGNCC 1 cut(s) 848
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 1 cut(s) 500
PteI GCGCGC 2 cut(s) 61, 63
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 4 cut(s) 659, 676, 1223, 1393
RsaNI GTAC 4 cut(s) 658, 675, 1222, 1392
RseI CAYNNNNRTG 1 cut(s) 1075
SacI GAGCTC 1 cut(s) 546
SalI GTCGAC 1 cut(s) 1262
SaqAI TTAA 7 cut(s) 431, 633, 756, 972, 1053, 1313, 1367
Sau3AI GATC 3 cut(s) 154, 526, 1537
Sau96I GGNCC 1 cut(s) 848
SchI GAGTC 4 cut(s) 140, 417, 1156, 1193
ScrFI CCNGG 1 cut(s) 936
SduI GDGCHC 1 cut(s) 546
SfaNI GCATC 3 cut(s) 712, 851, 1015
SfcI CTRYAG 2 cut(s) 376, 612
SinI GGWCC 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 1075
SnaBI TACGTA 2 cut(s) 674, 1046
SpeI ACTAGT 1 cut(s) 1234
SphI GCATGC 1 cut(s) 1015
Sse9I AATT 5 cut(s) 693, 835, 1118, 1127, 1306
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 8 cut(s) 51, 162, 165, 373, 803, 1073, 1089, 1322
SspMI CTAG 5 cut(s) 741, 923, 962, 1235, 1558
SstI GAGCTC 1 cut(s) 546
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 934
StyI CCWWGG 3 cut(s) 150, 412, 922
TaaI ACNGT 6 cut(s) 75, 131, 380, 829, 1068, 1214
TaiI ACGT 4 cut(s) 676, 1048, 1269, 1548
TaqI TCGA 9 cut(s) 41, 195, 246, 440, 629, 859, 1060, 1263, 1536
TasI AATT 5 cut(s) 693, 835, 1118, 1127, 1306
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 3 cut(s) 53, 165, 168
TfiI GAWTC 4 cut(s) 181, 1057, 1397, 1445
Tru1I TTAA 7 cut(s) 431, 633, 756, 972, 1053, 1313, 1367
Tru9I TTAA 7 cut(s) 431, 633, 756, 972, 1053, 1313, 1367
TscAI CASTG 3 cut(s) 580, 1082, 1164
TseFI GTSAC 1 cut(s) 1068
Tsp45I GTSAC 1 cut(s) 1068
TspDTI ATGAA 1 cut(s) 1152
TspGWI ACGGA 3 cut(s) 316, 1054, 1155
TspRI CASTG 3 cut(s) 580, 1082, 1164
Van91I CCANNNNNTGG 1 cut(s) 1477
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 2 cut(s) 1127, 1306
XceI RCATGY 3 cut(s) 897, 1015, 1256
XmaJI CCTAGG 1 cut(s) 922
XmiI GTMKAC 1 cut(s) 1263
XspI CTAG 5 cut(s) 741, 923, 962, 1235, 1558
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.