Rorug05G0190100

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012535
Physical Location & Seq
Forward (+)
18514005 .. 18516521
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0190100.1

Sequence Viewer

Length: 1548 bp
ATGGCAATTCTCATAAACTCGGTGTCACCTATAACAAACCCATCAGCAGAAACTGCTAGAAAGGGTTGTGGGTTCTTTTCCCACATCAGGGTCCAGACATTTTTACTCAACAAGGGGTTTTCAAGAGTTTTGGCATCAACCCAGATTACCATTTCTCCAAAGGACACTGTTTTCACTTTGCCCAATTGGAGGAATCCCAAGAATGATAGAAGAAGTAGAGAAGTTAGAATAATTGATGCCTTTCAGCATTTAGAGTCTATGATAGGGAAGGGCCAAAAGCCTGATGTGGGCCAAGCAACTCAGCTCTTGTATGATCTGTGCAAGGCGAGTAAGATGAGGAAAGCGGTGAGAGTGATCGAAATGATGGTTGCTTCAGGCATTATACCTGATGCAGCTTCATATACCTTCTTGGTGAACTATTTGTGTAAAAGAGGGAATATTGGTTATGCAATGCAGTTGGTTGAGAAGATGGAGGAGTATGGATATCCGACGAATACTGTTACCTATAATTCACTTGTTAGAGGGCTTTGTGTGCGTGGAAATTTGAATCAAAGCTTGCAGCTTTTGGATAAGCTGATACGGAAGGGGCTGGTTCCGAATGTTTATACTTACTCTTTCTTGCTTGAAGCGGCTTATAAGGAAAGAGGGGTCAACGAAGCGATGAAGCTCCTGGAGGAGATCATTGCTAAAGGTGGAAAGCCTAATTTGGTTAGTTACAATGTTTTGTTGACTGGTTTGTGCAAGGAAGGTAGGAGTGATGAGGCAATTAAGTTTTTTAGGAGTTTGCCTTCGATGGGATTCAGACCGAATGTTGTGAGCTATAACATTGTGCTGAGGAGCTTGTGTTATGAGGGTAGGTGGGAAGAGGCGAATGTGCTTTTAGCTGAGATGGATGGTGAGGATCGGACACCCTCCATAGTTACATATAACATCTTAATCAGTTCTCTTGCCCTTCATGGTAGAACAGAACATGCTCTTGAAGTTTTGGATGAAATGGTTAAGGGTCGGTTCAAGCCTACTGCTGCAAGCTACAATCCGATAATTACTCGTCTCTGCAAAGAGGGGAAGGTGGATCATGTGGTGAAGTGTCTGGACCAAATGATGTTCCGGCGATGTAATCCAAATGAGGGAACATTCAATGCCATTGCTGTGCTATGTGAGCAAGGTATGGTGCAAGAAGCATACTCCATCATTCAAAGCCTCGGAAATAAACAGAAATGCTCCACGAATGAGTTCCACAGAAATGTAATCACAAGCTTGTGCAGGAAAGGGAATACATATCCAGCATTTCAGATGTTATATGAAATGACCAAGTATGGTTTTACTCCAGATTCTTATACCTATTCATCTTTGATCAGAGGGTTGTGTCTGGAGGGTATGCTAAATGAAGCAATGGAGATTTTCAAGGTCATGGAGGAAAACAACCAAAGGCCTGATACTGAGAATTTCAATGCCCTTATACTTGGATTTTGCAAATGTCGAAGAACAGATCTATCCTTGCAGGTTTTCGAGATGATGATTGAGAAAAGGCTAATGCCTAATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

515

Amino Acids

58.31

Weight (kDa)

8.93

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 106 - 143 6e-07 PPR repeat family
PPR_long PF17177 110 - 212 1.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 126 - 158 6.2e-07 PPR repeat
PPR_2 PF13041 129 - 177 4.8e-12 PPR repeat family
TPR_24 PF23276 145 - 261 7.9e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 153 - 210 9.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 162 - 191 2.7e-06 PPR repeat
PPR_2 PF13041 165 - 210 9.5e-12 PPR repeat family
PPR_2 PF13041 207 - 248 1e-07 PPR repeat family
PPR_3 PF13812 222 - 279 8.8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 230 - 260 4.7e-10 PPR repeat
PPR_2 PF13041 234 - 282 3.7e-15 PPR repeat family
PPR PF01535 237 - 267 1.3e-06 PPR repeat
PPR_1 PF12854 265 - 297 1.8e-07 PPR repeat
PPR_2 PF13041 269 - 317 5.7e-13 PPR repeat family
PPR_3 PF13812 293 - 349 4.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 304 - 333 1.8e-08 PPR repeat
PPR_2 PF13041 304 - 348 3.6e-10 PPR repeat family
PPR PF01535 307 - 334 4.5e-07 PPR repeat
PPR_2 PF13041 339 - 381 8.2e-09 PPR repeat family
TPR_24 PF23276 387 - 504 8.1e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 416 - 456 3.3e-11 PPR repeat family
PPR_3 PF13812 433 - 487 6.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 440 - 472 1.8e-11 PPR repeat
PPR PF01535 446 - 475 2.1e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
Acc36I ACCTGC 1 cut(s) 1492
AciI CCGC 2 cut(s) 344, 629
AclWI GGATC 2 cut(s) 909, 1080
AcsI RAATTY 2 cut(s) 541, 1444
AcuI CTGAAG 1 cut(s) 357
AfiI CCNNNNNNNGG 6 cut(s) 87, 88, 189, 287, 794, 1127
AgsI TTSAA 9 cut(s) 123, 547, 626, 980, 1012, 1138, 1196, 1405, 1450
AjnI CCWGG 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 1055
AlwI GGATC 2 cut(s) 909, 1080
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 3 cut(s) 271, 289, 1430
ApeKI GCWGC 3 cut(s) 392, 559, 1022
ApoI RAATTY 2 cut(s) 541, 1444
Asp700I GAANNNNTTC 1 cut(s) 1232
AspS9I GGNCC 4 cut(s) 91, 271, 289, 1093
AsuHPI GGTGA 5 cut(s) 18, 358, 424, 908, 1093
AvaII GGWCC 2 cut(s) 91, 1093
BbvCI CCTCAGC 1 cut(s) 833
BbvI GCAGC 3 cut(s) 404, 571, 1009
BccI CCATC 7 cut(s) 49, 358, 463, 787, 883, 887, 1196
BciT130I CCWGG 1 cut(s) 671
BclI TGATCA 1 cut(s) 1353
BcoDI GTCTC 1 cut(s) 1055
BfaI CTAG 1 cut(s) 57
BfuAI ACCTGC 1 cut(s) 1492
BglII AGATCT 1 cut(s) 1489
BisI GCNGC 4 cut(s) 393, 560, 630, 1023
BlsI GCNGC 4 cut(s) 394, 561, 631, 1024
Bme1390I CCNGG 1 cut(s) 671
Bme18I GGWCC 2 cut(s) 91, 1093
BmgT120I GGNCC 4 cut(s) 91, 271, 289, 1093
BmiI GGNNCC 2 cut(s) 92, 594
BmrFI CCNGG 1 cut(s) 671
BmsI GCATC 3 cut(s) 143, 226, 379
BpmI CTGGAG 3 cut(s) 692, 1311, 1391
Bpu10I CCTNAGC 1 cut(s) 833
BsaJI CCNNGG 1 cut(s) 1201
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 829, 859
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 88, 189, 287, 794, 1127
Bse1I ACTGG 1 cut(s) 736
Bse3DI GCAATG 4 cut(s) 456, 681, 1143, 1398
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 1 cut(s) 1201
BseGI GGATG 2 cut(s) 898, 994
BseLI CCNNNNNNNGG 6 cut(s) 87, 88, 189, 287, 794, 1127
BseMI GCAATG 4 cut(s) 456, 681, 1143, 1398
BseMII CTCAG 4 cut(s) 314, 824, 876, 1431
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 3 cut(s) 488, 689, 850
BseXI GCAGC 3 cut(s) 404, 571, 1009
BsgI GTGCAG 1 cut(s) 1282
BshFI GGCC 3 cut(s) 273, 291, 1432
BsiSI CCGG 1 cut(s) 1108
BslI CCNNNNNNNGG 6 cut(s) 87, 88, 189, 287, 794, 1127
BsmAI GTCTC 1 cut(s) 1055
BsmBI CGTCTC 1 cut(s) 1055
BsnI GGCC 3 cut(s) 273, 291, 1432
Bsp143I GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
BspACI CCGC 2 cut(s) 344, 629
BspANI GGCC 3 cut(s) 273, 291, 1432
BspCNI CTCAG 4 cut(s) 313, 825, 877, 1432
BspLI GGNNCC 2 cut(s) 92, 594
BspMI ACCTGC 1 cut(s) 1492
BspPI GGATC 2 cut(s) 909, 1080
BsrDI GCAATG 4 cut(s) 456, 681, 1143, 1398
BsrI ACTGG 1 cut(s) 736
BssECI CCNNGG 1 cut(s) 1201
BssMI GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 2 cut(s) 169, 499
Bst6I CTCTTC 1 cut(s) 858
BstAPI GCANNNNNTGC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 557, 1027
BstDEI CTNAG 4 cut(s) 300, 833, 885, 1440
BstF5I GGATG 2 cut(s) 898, 994
BstKTI GATC 7 cut(s) 316, 357, 681, 904, 1075, 1356, 1492
BstMAI GTCTC 1 cut(s) 1055
BstMBI GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
BstMWI GCNNNNNNNGC 3 cut(s) 53, 532, 1159
BstNI CCWGG 1 cut(s) 671
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 1 cut(s) 669
BstV1I GCAGC 3 cut(s) 404, 571, 1009
BstX2I RGATCY 1 cut(s) 1489
BstYI RGATCY 1 cut(s) 1489
BsuRI GGCC 3 cut(s) 273, 291, 1432
BtgZI GCGATG 2 cut(s) 674, 1126
BtsCI GGATG 2 cut(s) 898, 994
BtsIMutI CAGTG 1 cut(s) 165
BveI ACCTGC 1 cut(s) 1492
Cac8I GCNNGC 2 cut(s) 557, 1027
CaiI CAGNNNCTG 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 91, 271, 289, 1093
CviAII CATG 4 cut(s) 956, 971, 1076, 1411
DdeI CTNAG 4 cut(s) 300, 833, 885, 1440
DpnI GATC 7 cut(s) 315, 356, 680, 903, 1074, 1355, 1491
DpnII GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
Eco147I AGGCCT 1 cut(s) 1432
Eco32I GATATC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 91, 1093
Eco57I CTGAAG 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 669
EcoRV GATATC 1 cut(s) 485
Esp3I CGTCTC 1 cut(s) 1055
FaeI CATG 4 cut(s) 959, 974, 1079, 1414
FatI CATG 4 cut(s) 955, 970, 1075, 1410
FbaI TGATCA 1 cut(s) 1353
Fnu4HI GCNGC 4 cut(s) 393, 560, 630, 1023
FokI GGATG 2 cut(s) 905, 1001
Fsp4HI GCNGC 4 cut(s) 393, 560, 630, 1023
FspBI CTAG 1 cut(s) 57
GluI GCNGC 4 cut(s) 393, 560, 630, 1023
GsuI CTGGAG 3 cut(s) 692, 1311, 1391
HaeIII GGCC 3 cut(s) 273, 291, 1432
HapII CCGG 1 cut(s) 1108
Hin1II CATG 4 cut(s) 959, 974, 1079, 1414
HincII GTYRAC 2 cut(s) 652, 729
HindII GTYRAC 2 cut(s) 652, 729
HindIII AAGCTT 2 cut(s) 553, 1255
HinfI GANTC 5 cut(s) 193, 254, 547, 798, 1331
HpaII CCGG 1 cut(s) 1108
HphI GGTGA 5 cut(s) 18, 358, 424, 908, 1093
Hpy166II GTNNAC 3 cut(s) 415, 652, 729
Hpy188I TCNGA 8 cut(s) 489, 597, 803, 906, 1038, 1205, 1293, 1358
Hpy188III TCNNGA 7 cut(s) 94, 123, 977, 1091, 1328, 1370, 1510
Hpy8I GTNNAC 3 cut(s) 415, 652, 729
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 7 cut(s) 262, 415, 577, 740, 798, 962, 1060
HpyCH4III ACNGT 2 cut(s) 169, 499
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 532, 1159
HpyF3I CTNAG 4 cut(s) 300, 833, 885, 1440
Hsp92II CATG 4 cut(s) 959, 974, 1079, 1414
Ksp22I TGATCA 1 cut(s) 1353
Kzo9I GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
LmnI GCTCC 3 cut(s) 672, 837, 1226
Lsp1109I GCAGC 3 cut(s) 404, 571, 1009
LweI GCATC 3 cut(s) 143, 226, 379
MaeI CTAG 1 cut(s) 57
MaeIII GTNAC 4 cut(s) 24, 499, 713, 919
MalI GATC 7 cut(s) 315, 356, 680, 903, 1074, 1355, 1491
MboI GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
MboII GAAGA 4 cut(s) 222, 478, 875, 1494
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 1489
MluCI AATT 9 cut(s) 6, 184, 231, 508, 541, 703, 765, 1041, 1444
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 512
MroXI GAANNNNTTC 1 cut(s) 1232
MseI TTAA 3 cut(s) 768, 935, 999
MslI CAYNNNNRTG 2 cut(s) 1148, 1242
MspI CCGG 1 cut(s) 1108
MspR9I CCNGG 1 cut(s) 671
MunI CAATTG 1 cut(s) 184
MvaI CCWGG 1 cut(s) 671
MwoI GCNNNNNNNGC 3 cut(s) 53, 532, 1159
NdeII GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
NlaIII CATG 4 cut(s) 959, 974, 1079, 1414
NlaIV GGNNCC 2 cut(s) 92, 594
NmuCI GTSAC 1 cut(s) 24
NspI RCATGY 1 cut(s) 974
PceI AGGCCT 1 cut(s) 1432
PdmI GAANNNNTTC 1 cut(s) 1232
PfeI GAWTC 4 cut(s) 193, 547, 798, 1331
PfoI TCCNGGA 1 cut(s) 669
PkrI GCNGC 4 cut(s) 394, 561, 631, 1024
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PsiI TTATAA 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 669
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 92, 594
PspPI GGNCC 4 cut(s) 91, 271, 289, 1093
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 1489
RseI CAYNNNNRTG 2 cut(s) 1148, 1242
SaqAI TTAA 3 cut(s) 768, 935, 999
SatI GCNGC 4 cut(s) 393, 560, 630, 1023
Sau3AI GATC 7 cut(s) 313, 354, 678, 901, 1072, 1353, 1489
Sau96I GGNCC 4 cut(s) 91, 271, 289, 1093
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 671
SfaNI GCATC 3 cut(s) 143, 226, 379
SinI GGWCC 2 cut(s) 91, 1093
SmiMI CAYNNNNRTG 2 cut(s) 1148, 1242
Sse9I AATT 9 cut(s) 6, 184, 231, 508, 541, 703, 765, 1041, 1444
SseBI AGGCCT 1 cut(s) 1432
SsiI CCGC 2 cut(s) 344, 629
SspI AATATT 1 cut(s) 439
SspMI CTAG 1 cut(s) 57
StuI AGGCCT 1 cut(s) 1432
StyD4I CCNGG 1 cut(s) 669
TaaI ACNGT 2 cut(s) 169, 499
TaqI TCGA 4 cut(s) 357, 791, 1480, 1509
TaqII GACCGA 1 cut(s) 820
TasI AATT 9 cut(s) 6, 184, 231, 508, 541, 703, 765, 1041, 1444
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 4 cut(s) 193, 547, 798, 1331
Tru1I TTAA 3 cut(s) 768, 935, 999
Tru9I TTAA 3 cut(s) 768, 935, 999
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 24
TseI GCWGC 3 cut(s) 392, 559, 1022
Tsp45I GTSAC 1 cut(s) 24
TspDTI ATGAA 7 cut(s) 387, 677, 944, 1005, 1317, 1335, 1401
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 2 cut(s) 91, 1093
XapI RAATTY 2 cut(s) 541, 1444
XceI RCATGY 1 cut(s) 974
XmnI GAANNNNTTC 1 cut(s) 1232
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.