Rmu_sc0009425.1_g000001

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009425.1
OG0012535
Physical Location & Seq
Reverse (-)
2 .. 3902
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009425.1_g000001.1.cds

Sequence Viewer

Length: 1129 bp
atggccgccgccgacggtggagaagtcagtgcaattctccgcgacctcgaaagcttgaaggcgagtaagagcgaaatcgagctccgaatctcggccctcgaagctcgcctgcaagagattaaccttcagcagccgaacggtgccgtttcgaatggctcttgtgctcctctgccgtcgtccgttgattccggctacggtcacgatttgtcgcctcagatgatttaccgctacagtcgccagctcttgcttccctctttcggagtccaagctcagtcgaagctcctaaagtcatcgattttggtggttggtgctggagggttgggctcgccggctctgctatatcttgcagcttgtggtgttggccacttggggattattgatcatgatgtggttgagctcaacaatatgcacaggcagattatccacactgaaagatttattggtcagcccaaagtgagatctgctgcagctgcttgtcgatcgattaactccactatccaggttgtggaataccaagaaccattacgcacatccaatgctttggaaatcatgagcaaatatgatataatagtggatgccacggacaatgctcctagccgttacatgataagcgattgctgtgtggtgctagggaagcctcttgtatctggtgccgcactcggatttgaagggcagctcacggtgtacaattacaatggtggtccatgctatcgatgcctatttccaactcctccacctacaacagcatgtcaaaggtgttctgatagtggtgtactaggtgtagttccaggtatcattggttgtctccaagccctagaagctattaagattgcaggtgaagttggtgaaccactctcaggacgaatgcttttacttgattcattgtcaggacggattcgaattgttaagatccgaggaaggtccttgcaatgcgtagcttgtggtgaaaatacaacattctctaagcagcaatttcgggaatttgattatgaaaacttcactcagtctccactaactccgtcacctttgaagttaaacctacttcaggcagactccagaataaatactaaagagtacaaggagaaaattgatgacggggaagcacatgttttgattgatgttcgtccag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

376

Amino Acids

40.53

Weight (kDa)

6.11

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 824
Acc36I ACCTGC 1 cut(s) 824
AccB1I GGYRCC 2 cut(s) 140, 650
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 42
AciI CCGC 5 cut(s) 6, 9, 40, 226, 654
AclWI GGATC 1 cut(s) 904
AcoI YGGCCR 2 cut(s) 3, 361
AcsI RAATTY 1 cut(s) 980
AcuI CTGAAG 2 cut(s) 110, 1028
AfaI GTAC 3 cut(s) 686, 774, 1076
AfiI CCNNNNNNNGG 5 cut(s) 91, 257, 505, 857, 1045
AflIII ACRYGT 1 cut(s) 1105
AgsI TTSAA 3 cut(s) 58, 668, 1030
AjnI CCWGG 2 cut(s) 498, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
Alw21I GWGCWC 3 cut(s) 84, 166, 399
Alw26I GTCTC 2 cut(s) 809, 1011
AlwI GGATC 1 cut(s) 904
AoxI GGCC 3 cut(s) 3, 93, 361
ApeKI GCWGC 7 cut(s) 130, 347, 464, 467, 470, 673, 967
ApoI RAATTY 1 cut(s) 980
AspS9I GGNCC 3 cut(s) 94, 701, 921
AsuHPI GGTGA 4 cut(s) 848, 857, 956, 1014
AsuII TTCGAA 2 cut(s) 149, 898
AvaII GGWCC 2 cut(s) 701, 921
BalI TGGCCA 1 cut(s) 363
BanI GGYRCC 2 cut(s) 140, 650
BanII GRGCYC 3 cut(s) 84, 326, 399
Bbv12I GWGCWC 3 cut(s) 84, 166, 399
BbvI GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
BceAI ACGGC 3 cut(s) 128, 157, 582
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 2 cut(s) 500, 789
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 809, 1011
BfaI CTAG 4 cut(s) 594, 629, 776, 815
BfmI CTRYAG 2 cut(s) 229, 465
BfuAI ACCTGC 1 cut(s) 824
BglII AGATCT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 500, 789
Bme18I GGWCC 2 cut(s) 701, 921
BmgT120I GGNCC 3 cut(s) 94, 701, 921
BmiI GGNNCC 2 cut(s) 142, 652
BmrFI CCNGG 2 cut(s) 500, 789
BmsI GCATC 2 cut(s) 565, 704
BpmI CTGGAG 2 cut(s) 333, 1039
Bpu14I TTCGAA 2 cut(s) 149, 898
Bsa29I ATCGAT 3 cut(s) 293, 482, 712
BsaJI CCNNGG 2 cut(s) 579, 913
BsaXI ACNNNNNCTCC 2 cut(s) 991, 1021
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 257, 505, 857, 1045
Bse118I RCCGGY 1 cut(s) 328
Bse3DI GCAATG 1 cut(s) 935
BseBI CCWGG 2 cut(s) 500, 789
BseCI ATCGAT 3 cut(s) 293, 482, 712
BseDI CCNNGG 2 cut(s) 579, 913
BseGI GGATG 2 cut(s) 530, 580
BseLI CCNNNNNNNGG 5 cut(s) 91, 257, 505, 857, 1045
BseMI GCAATG 1 cut(s) 935
BseMII CTCAG 4 cut(s) 227, 284, 870, 1016
BseRI GAGGAG 2 cut(s) 156, 720
BseXI GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
Bsh1236I CGCG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 3 cut(s) 5, 95, 363
BshNI GGYRCC 2 cut(s) 140, 650
BshVI ATCGAT 3 cut(s) 293, 482, 712
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 3 cut(s) 84, 166, 399
BsiSI CCGG 2 cut(s) 189, 329
BslI CCNNNNNNNGG 5 cut(s) 91, 257, 505, 857, 1045
BsmAI GTCTC 2 cut(s) 809, 1011
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 3 cut(s) 5, 95, 363
Bsp119I TTCGAA 2 cut(s) 149, 898
Bsp1286I GDGCHC 4 cut(s) 84, 166, 326, 399
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 4 cut(s) 379, 458, 479, 909
BspACI CCGC 5 cut(s) 6, 9, 40, 226, 654
BspANI GGCC 3 cut(s) 5, 95, 363
BspCNI CTCAG 4 cut(s) 226, 283, 869, 1015
BspDI ATCGAT 3 cut(s) 293, 482, 712
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 2 cut(s) 382, 549
BspLI GGNNCC 2 cut(s) 142, 652
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 1 cut(s) 904
BspT104I TTCGAA 2 cut(s) 149, 898
BspT107I GGYRCC 2 cut(s) 140, 650
BsrDI GCAATG 1 cut(s) 935
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 2 cut(s) 579, 913
BssMI GATC 4 cut(s) 379, 458, 479, 909
Bst2UI CCWGG 2 cut(s) 500, 789
Bst4CI ACNGT 5 cut(s) 17, 140, 197, 233, 682
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 2 cut(s) 149, 898
BstC8I GCNNGC 5 cut(s) 106, 110, 239, 326, 330
BstDEI CTNAG 5 cut(s) 213, 270, 856, 963, 1002
BstDSI CCRYGG 1 cut(s) 579
BstENI CCTNNNNNAGG 1 cut(s) 1043
BstF5I GGATG 2 cut(s) 530, 580
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 4 cut(s) 382, 461, 482, 912
BstMAI GTCTC 2 cut(s) 809, 1011
BstMBI GATC 4 cut(s) 379, 458, 479, 909
BstMCI CGRYCG 1 cut(s) 482
BstMWI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 818
BstNI CCWGG 2 cut(s) 500, 789
BstNSI RCATGY 2 cut(s) 750, 1109
BstSCI CCNGG 2 cut(s) 498, 787
BstSFI CTRYAG 2 cut(s) 229, 465
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
BstX2I RGATCY 2 cut(s) 458, 909
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 2 cut(s) 458, 909
Bsu15I ATCGAT 3 cut(s) 293, 482, 712
BsuRI GGCC 3 cut(s) 5, 95, 363
BsuTUI ATCGAT 3 cut(s) 293, 482, 712
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 2 cut(s) 530, 580
BtsIMutI CAGTG 2 cut(s) 34, 426
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 5 cut(s) 106, 110, 239, 326, 330
CciI TCATGA 2 cut(s) 382, 549
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 94, 701, 921
ClaI ATCGAT 3 cut(s) 293, 482, 712
Csp6I GTAC 3 cut(s) 685, 773, 1075
CviAII CATG 6 cut(s) 383, 550, 604, 705, 747, 1106
CviQI GTAC 3 cut(s) 685, 773, 1075
DdeI CTNAG 5 cut(s) 213, 270, 856, 963, 1002
DpnI GATC 4 cut(s) 381, 460, 481, 911
DpnII GATC 4 cut(s) 379, 458, 479, 909
EaeI YGGCCR 2 cut(s) 3, 361
Ecl136II GAGCTC 2 cut(s) 82, 397
Eco24I GRGCYC 3 cut(s) 84, 326, 399
Eco47I GGWCC 2 cut(s) 701, 921
Eco53kI GAGCTC 2 cut(s) 82, 397
Eco57I CTGAAG 2 cut(s) 110, 1028
EcoICRI GAGCTC 2 cut(s) 82, 397
EcoNI CCTNNNNNAGG 1 cut(s) 1043
EcoO109I RGGNCCY 1 cut(s) 921
EcoRII CCWGG 2 cut(s) 498, 787
EcoT38I GRGCYC 3 cut(s) 84, 326, 399
FaeI CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
FatI CATG 6 cut(s) 382, 549, 603, 704, 746, 1105
FbaI TGATCA 1 cut(s) 379
FokI GGATG 2 cut(s) 517, 587
FriOI GRGCYC 3 cut(s) 84, 326, 399
FspBI CTAG 4 cut(s) 594, 629, 776, 815
GsuI CTGGAG 2 cut(s) 333, 1039
HaeIII GGCC 3 cut(s) 5, 95, 363
HapII CCGG 2 cut(s) 189, 329
Hin1II CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 6 cut(s) 87, 185, 261, 878, 895, 1052
HpaII CCGG 2 cut(s) 189, 329
HphI GGTGA 4 cut(s) 848, 857, 956, 1014
Hpy166II GTNNAC 3 cut(s) 685, 773, 848
Hpy188I TCNGA 6 cut(s) 86, 216, 260, 662, 763, 914
Hpy188III TCNNGA 7 cut(s) 200, 383, 550, 858, 888, 977, 1056
Hpy8I GTNNAC 3 cut(s) 685, 773, 848
Hpy99I CGWCG 2 cut(s) 17, 178
HpyAV CCTTC 4 cut(s) 52, 134, 662, 912
HpyCH4III ACNGT 5 cut(s) 17, 140, 197, 233, 682
HpyCH4V TGCA 7 cut(s) 32, 112, 347, 409, 467, 833, 928
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 818
HpyF3I CTNAG 5 cut(s) 213, 270, 856, 963, 1002
Hsp92II CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 4 cut(s) 379, 458, 479, 909
LmnI GCTCC 4 cut(s) 87, 169, 285, 595
Lsp1109I GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
LweI GCATC 2 cut(s) 565, 704
MaeI CTAG 4 cut(s) 594, 629, 776, 815
MaeIII GTNAC 3 cut(s) 197, 599, 1020
MalI GATC 4 cut(s) 381, 460, 481, 911
MboI GATC 4 cut(s) 379, 458, 479, 909
MflI RGATCY 2 cut(s) 458, 909
MhlI GDGCHC 4 cut(s) 84, 166, 326, 399
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 6 cut(s) 33, 688, 900, 971, 980, 1086
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 2 cut(s) 270, 1046
MmeI TCCRAC 1 cut(s) 749
MnlI CCTC 9 cut(s) 56, 107, 177, 222, 262, 308, 648, 741, 908
Mox20I TGGCCA 1 cut(s) 363
MroNI GCCGGC 1 cut(s) 328
MscI TGGCCA 1 cut(s) 363
MseI TTAA 5 cut(s) 120, 486, 825, 906, 1034
Msp20I TGGCCA 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 189, 329
MspR9I CCNGG 2 cut(s) 500, 789
Mva1269I GAATGC 1 cut(s) 870
MvaI CCWGG 2 cut(s) 500, 789
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 818
NaeI GCCGGC 1 cut(s) 330
NdeII GATC 4 cut(s) 379, 458, 479, 909
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
NlaIV GGNNCC 2 cut(s) 142, 652
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 2 cut(s) 197, 1020
NspI RCATGY 2 cut(s) 750, 1109
NspV TTCGAA 2 cut(s) 149, 898
PagI TCATGA 2 cut(s) 382, 549
PaqCI CACCTGC 1 cut(s) 824
PciI ACATGT 1 cut(s) 1105
PctI GAATGC 1 cut(s) 870
PdiI GCCGGC 1 cut(s) 330
PfeI GAWTC 4 cut(s) 87, 185, 878, 895
PflMI CCANNNNNTGG 1 cut(s) 505
Ple19I CGATCG 1 cut(s) 482
PleI GAGTC 2 cut(s) 269, 1046
PpsI GAGTC 2 cut(s) 269, 1046
PpuMI RGGWCCY 1 cut(s) 921
PscI ACATGT 1 cut(s) 1105
Psp124BI GAGCTC 2 cut(s) 84, 399
Psp5II RGGWCCY 1 cut(s) 921
Psp6I CCWGG 2 cut(s) 498, 787
PspGI CCWGG 2 cut(s) 498, 787
PspN4I GGNNCC 2 cut(s) 142, 652
PspPI GGNCC 3 cut(s) 94, 701, 921
PspPPI RGGWCCY 1 cut(s) 921
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 2 cut(s) 458, 909
PvuI CGATCG 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 3 cut(s) 686, 774, 1076
RsaNI GTAC 3 cut(s) 685, 773, 1075
SacI GAGCTC 2 cut(s) 84, 399
SaqAI TTAA 5 cut(s) 120, 486, 825, 906, 1034
Sau3AI GATC 4 cut(s) 379, 458, 479, 909
Sau96I GGNCC 3 cut(s) 94, 701, 921
SchI GAGTC 2 cut(s) 270, 1046
ScrFI CCNGG 2 cut(s) 500, 789
SduI GDGCHC 4 cut(s) 84, 166, 326, 399
SfaNI GCATC 2 cut(s) 565, 704
SfcI CTRYAG 2 cut(s) 229, 465
SfuI TTCGAA 2 cut(s) 149, 898
SinI GGWCC 2 cut(s) 701, 921
Sse9I AATT 6 cut(s) 33, 688, 900, 971, 980, 1086
SsiI CCGC 5 cut(s) 6, 9, 40, 226, 654
SspMI CTAG 4 cut(s) 594, 629, 776, 815
SstI GAGCTC 2 cut(s) 84, 399
StyD4I CCNGG 2 cut(s) 498, 787
TaaI ACNGT 5 cut(s) 17, 140, 197, 233, 682
TasI AATT 6 cut(s) 33, 688, 900, 971, 980, 1086
TatI WGTACW 3 cut(s) 684, 772, 1074
TauI GCSGC 3 cut(s) 8, 11, 656
TfiI GAWTC 4 cut(s) 87, 185, 878, 895
Tru1I TTAA 5 cut(s) 120, 486, 825, 906, 1034
Tru9I TTAA 5 cut(s) 120, 486, 825, 906, 1034
TscAI CASTG 2 cut(s) 34, 433
TseFI GTSAC 2 cut(s) 197, 1020
TseI GCWGC 7 cut(s) 130, 347, 464, 467, 470, 673, 967
Tsp45I GTSAC 2 cut(s) 197, 1020
TspDTI ATGAA 2 cut(s) 870, 1005
TspGWI ACGGA 4 cut(s) 169, 596, 907, 1008
TspRI CASTG 2 cut(s) 34, 433
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 701, 921
XagI CCTNNNNNAGG 1 cut(s) 1043
XapI RAATTY 1 cut(s) 980
XceI RCATGY 2 cut(s) 750, 1109
XspI CTAG 4 cut(s) 594, 629, 776, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.