Prupe.4G221100_v2.0.a1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012535
Physical Location & Seq
Forward (+)
13890919 .. 13895083
4165 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G221100.1

Sequence Viewer

Length: 1410 bp
ATGGATCCCGACTGTGGAGAAGCTTCTAGGATTCTTCTCCAACTCGAAACCTTGAAGTCCACTAAGACCGAAATAGAGCGCCAAATCTCAGCCCTCGAAGCTCAGCTCCAAGAGATTAACCTGCAGCATCCAAACGCCACCGTTCCAAATGGTTCTTGTCCTCCTGTAATCCCGTCCGTTGATTCGGGTTACGGTCACGATTTGTCACCTCAGATGATTTACCGCTACAGTCGCCACCTCTTGCTCCCTGCCTTCGGAGTCCAAGGGCAATCAAAGTTGTTAAAGTCGTCGGTTTTGGTGGTCGGAGCCGGAGGGTTGGGCTCGCCTGCTCTGCTATATCTTGCAGCTTGTGGTGTTGGCCGCTTGGGGATTGTTGATCATGATGTGGTTGAGCTCAATAATATGCACAGGCAGATTATCCACACTGAAGGTTTTATTGGTCAGCCAAAAGTGAGATCTGCTGCTACTGCTTGTCGTTCGATTAACTCCACTATTCAGGTTGTGGAATACCAAGAACCTTTACGTACATCCAATGCTTTAGAAATCTTGAGCAAATATGATATAATAGTAGATGCAACAGACAATGCTCCTAGCCGTTACATGATTAGCGATTGTTGTGTGGTGTTAGGGAAGCCTCTTGTATCTGGTGCTGCCGTGGGATTGGAAGGGCAGCTCACAGTTTTCAATTACAATGACGGCCCATGCTATCGTTGCCTATTTCCAACTCCACCGCCTGCAACAGCATGTCAAAGATGTTCTGACAGTGGCGTCCTAGGCGTAGTTCCCGGTGTTATTGGCTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAAGTGAAATTGGTGAAGCACTCTCAGAAAGGATGCTTTTATTTGATGCATTGTCAGGACGGATACGAATTGTTAAGATTCGGGGAAGGTCATTGCAATGCGTAGCTTGTGGTGAAAATGCACCATTCTCTAAGCAGCAGTTTCAGGAATTTGATTATGAGAAGTTCACTCAGTCTCCACTGACTCCGTTACCTTTGAAGTTAAAGCTACTTCGGGCAGACTCCAGAATAAATACTAAAGAGTACAAGGAGAAACTTGTGAGCGGGGAAGCACATGTGTTGATCGATGTTCGTCCGGAACATCACTTCAAAATTGTTTCTCTGCCCAATTCCTTAAACATCCCGCTCCCGAGTTTGGAGTCTCGGTTGCCTGAAATCTCTTCAGCCCTAAAGGAAAAGGAAGAGCATCAAGGTACTGACTCTGGTTCAGGTGGGCGATTATATGTAATATGCAGAAGGGGTAATGATTCTCAGAGGGCTGTTCAGTTTCTTCACAAGATGGGTTTCACGTCGGCCAGAGACATCATTGGGGGACTGGAAGGTTGGGCCCATGATGTGGATCCAAACGTCCCTACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

470

Amino Acids

51.06

Weight (kDa)

6.33

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 1384
AccBSI CCGCTC 2 cut(s) 1092, 1174
AccIII TCCGGA 1 cut(s) 1123
AciI CCGC 5 cut(s) 223, 361, 731, 1092, 1172
AclWI GGATC 3 cut(s) 12, 1382, 1395
AcoI YGGCCR 2 cut(s) 358, 1341
AcsI RAATTY 1 cut(s) 977
AcuI CTGAAG 2 cut(s) 447, 1194
AcyI GRCGYC 1 cut(s) 768
AfaI GTAC 3 cut(s) 526, 1073, 1243
AfiI CCNNNNNNNGG 4 cut(s) 14, 254, 1183, 1384
AflIII ACRYGT 1 cut(s) 1102
AgsI TTSAA 4 cut(s) 55, 685, 1027, 1138
AjiI CACGTC 1 cut(s) 1338
AjuI GAANNNNNNNTTGG 2 cut(s) 420, 452
AluBI AGCT 9 cut(s) 23, 101, 106, 347, 394, 673, 818, 935, 1036
AluI AGCT 9 cut(s) 23, 101, 106, 347, 394, 673, 818, 935, 1036
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 4 cut(s) 806, 1008, 1194, 1341
AlwI GGATC 3 cut(s) 12, 1382, 1395
Ama87I CYCGRG 1 cut(s) 1177
Aor13HI TCCGGA 1 cut(s) 1123
AoxI GGCC 4 cut(s) 358, 697, 1341, 1374
ApaI GGGCCC 1 cut(s) 1378
ApeKI GCWGC 6 cut(s) 124, 344, 461, 650, 670, 964
ApoI RAATTY 1 cut(s) 977
AspA2I CCTAGG 1 cut(s) 772
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 3 cut(s) 698, 1374, 1375
AsuC2I CCSGG 1 cut(s) 786
AsuHPI GGTGA 3 cut(s) 198, 854, 953
AvaI CYCGRG 1 cut(s) 1177
AvrII CCTAGG 1 cut(s) 772
BaeGI GKGCMC 1 cut(s) 1378
BamHI GGATCC 2 cut(s) 4, 1387
BanII GRGCYC 3 cut(s) 323, 396, 1378
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 6 cut(s) 136, 356, 448, 637, 682, 976
BccI CCATC 1 cut(s) 1321
BceAI ACGGC 3 cut(s) 579, 638, 712
BciVI GTATCC 1 cut(s) 885
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 1 cut(s) 786
BcoDI GTCTC 4 cut(s) 806, 1008, 1194, 1341
BfaI CTAG 4 cut(s) 27, 591, 773, 812
BfmI CTRYAG 2 cut(s) 122, 226
BfoI RGCGCY 1 cut(s) 82
BfuAI ACCTGC 1 cut(s) 129
BfuI GTATCC 1 cut(s) 885
BglII AGATCT 1 cut(s) 455
BisI GCNGC 7 cut(s) 125, 345, 361, 462, 651, 671, 965
BlnI CCTAGG 1 cut(s) 772
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 7 cut(s) 126, 346, 362, 463, 652, 672, 966
Bme1390I CCNGG 1 cut(s) 786
BmeT110I CYCGRG 1 cut(s) 1177
BmgBI CACGTC 1 cut(s) 1338
BmgT120I GGNCC 3 cut(s) 698, 1374, 1375
BmiI GGNNCC 4 cut(s) 6, 307, 1376, 1389
BmrFI CCNGG 1 cut(s) 786
BmsI GCATC 5 cut(s) 136, 562, 852, 865, 1243
BpmI CTGGAG 1 cut(s) 1036
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 568
BpuMI CCSGG 1 cut(s) 786
Bsa29I ATCGAT 1 cut(s) 1113
BsaAI YACGTR 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 1386
BsaHI GRCGYC 1 cut(s) 768
BsaJI CCNNGG 3 cut(s) 262, 654, 772
BsaWI WCCGGW 1 cut(s) 1123
BsaXI ACNNNNNCTCC 4 cut(s) 988, 1018, 1178, 1208
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 254, 1183, 1384
Bse1I ACTGG 1 cut(s) 1368
Bse3DI GCAATG 2 cut(s) 920, 932
Bse8I GATNNNNATC 1 cut(s) 1386
BseAI TCCGGA 1 cut(s) 1123
BseCI ATCGAT 1 cut(s) 1113
BseDI CCNNGG 3 cut(s) 262, 654, 772
BseGI GGATG 4 cut(s) 127, 527, 867, 1167
BseJI GATNNNNATC 1 cut(s) 1386
BseLI CCNNNNNNNGG 4 cut(s) 14, 254, 1183, 1384
BseMI GCAATG 2 cut(s) 920, 932
BseMII CTCAG 6 cut(s) 102, 116, 224, 867, 1013, 1313
BseNI ACTGG 1 cut(s) 1368
BseSI GKGCMC 1 cut(s) 1378
BseXI GCAGC 6 cut(s) 136, 356, 448, 637, 682, 976
BshFI GGCC 4 cut(s) 360, 699, 1343, 1376
BshVI ATCGAT 1 cut(s) 1113
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 1177
BsiSI CCGG 3 cut(s) 309, 786, 1124
BslFI GGGAC 2 cut(s) 1374, 1382
BslI CCNNNNNNNGG 4 cut(s) 14, 254, 1183, 1384
BsmAI GTCTC 4 cut(s) 806, 1008, 1194, 1341
BsmFI GGGAC 2 cut(s) 1374, 1382
BsnI GGCC 4 cut(s) 360, 699, 1343, 1376
BsoBI CYCGRG 1 cut(s) 1177
Bsp120I GGGCCC 1 cut(s) 1374
Bsp1286I GDGCHC 3 cut(s) 323, 396, 1378
Bsp13I TCCGGA 1 cut(s) 1123
Bsp143I GATC 5 cut(s) 4, 376, 455, 1110, 1387
Bsp1720I GCTNAGC 1 cut(s) 102
BspACI CCGC 5 cut(s) 223, 361, 731, 1092, 1172
BspANI GGCC 4 cut(s) 360, 699, 1343, 1376
BspCNI CTCAG 6 cut(s) 101, 115, 223, 866, 1012, 1312
BspDI ATCGAT 1 cut(s) 1113
BspEI TCCGGA 1 cut(s) 1123
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 4 cut(s) 6, 307, 1376, 1389
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 129
BspPI GGATC 3 cut(s) 12, 1382, 1395
BspQI GCTCTTC 1 cut(s) 1224
BsrBI CCGCTC 2 cut(s) 1092, 1174
BsrDI GCAATG 2 cut(s) 920, 932
BsrI ACTGG 1 cut(s) 1368
BssECI CCNNGG 3 cut(s) 262, 654, 772
BssMI GATC 5 cut(s) 4, 376, 455, 1110, 1387
BssNI GRCGYC 1 cut(s) 768
BssT1I CCWWGG 2 cut(s) 262, 772
Bst4CI ACNGT 6 cut(s) 14, 142, 194, 230, 679, 764
Bst6I CTCTTC 2 cut(s) 1213, 1224
BstACI GRCGYC 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 524
BstC8I GCNNGC 3 cut(s) 323, 327, 735
BstDEI CTNAG 8 cut(s) 63, 88, 102, 210, 853, 960, 999, 1299
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 4 cut(s) 127, 527, 867, 1167
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 5 cut(s) 7, 379, 458, 1113, 1390
BstMAI GTCTC 4 cut(s) 806, 1008, 1194, 1341
BstMBI GATC 5 cut(s) 4, 376, 455, 1110, 1387
BstMWI GCNNNNNNNGC 7 cut(s) 98, 231, 331, 467, 711, 774, 815
BstNSI RCATGY 2 cut(s) 747, 1106
BstSCI CCNGG 1 cut(s) 784
BstSFI CTRYAG 2 cut(s) 122, 226
BstSLI GKGCMC 1 cut(s) 1378
BstSNI TACGTA 1 cut(s) 524
BstV1I GCAGC 6 cut(s) 136, 356, 448, 637, 682, 976
BstX2I RGATCY 3 cut(s) 4, 455, 1387
BstYI RGATCY 3 cut(s) 4, 455, 1387
Bsu15I ATCGAT 1 cut(s) 1113
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 4 cut(s) 360, 699, 1343, 1376
BsuTUI ATCGAT 1 cut(s) 1113
BtgI CCRYGG 1 cut(s) 654
BtrI CACGTC 1 cut(s) 1338
BtsCI GGATG 4 cut(s) 127, 527, 867, 1167
BtsIMutI CAGTG 3 cut(s) 423, 769, 1007
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 3 cut(s) 323, 327, 735
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 698, 1374, 1375
ClaI ATCGAT 1 cut(s) 1113
CseI GACGC 1 cut(s) 757
Csp6I GTAC 3 cut(s) 525, 1072, 1242
CviAII CATG 6 cut(s) 380, 601, 702, 744, 1103, 1379
CviQI GTAC 3 cut(s) 525, 1072, 1242
DdeI CTNAG 8 cut(s) 63, 88, 102, 210, 853, 960, 999, 1299
DpnI GATC 5 cut(s) 6, 378, 457, 1112, 1389
DpnII GATC 5 cut(s) 4, 376, 455, 1110, 1387
DrdI GACNNNNNNGTC 1 cut(s) 767
DseDI GACNNNNNNGTC 1 cut(s) 767
EaeI YGGCCR 2 cut(s) 358, 1341
Eam1104I CTCTTC 2 cut(s) 1213, 1224
EarI CTCTTC 2 cut(s) 1213, 1224
Ecl136II GAGCTC 1 cut(s) 394
Eco105I TACGTA 1 cut(s) 524
Eco130I CCWWGG 2 cut(s) 262, 772
Eco24I GRGCYC 3 cut(s) 323, 396, 1378
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 2 cut(s) 447, 1194
Eco88I CYCGRG 1 cut(s) 1177
EcoICRI GAGCTC 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 262, 772
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 3 cut(s) 323, 396, 1378
ErhI CCWWGG 2 cut(s) 262, 772
FaeI CATG 6 cut(s) 383, 604, 705, 747, 1106, 1382
FaqI GGGAC 2 cut(s) 1374, 1382
FatI CATG 6 cut(s) 379, 600, 701, 743, 1102, 1378
FauI CCCGC 2 cut(s) 1085, 1179
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 7 cut(s) 125, 345, 361, 462, 651, 671, 965
FokI GGATG 4 cut(s) 114, 514, 874, 1154
FriOI GRGCYC 3 cut(s) 323, 396, 1378
Fsp4HI GCNGC 7 cut(s) 125, 345, 361, 462, 651, 671, 965
FspBI CTAG 4 cut(s) 27, 591, 773, 812
GlaI GCGC 1 cut(s) 80
GluI GCNGC 7 cut(s) 125, 345, 361, 462, 651, 671, 965
GsuI CTGGAG 1 cut(s) 1036
HaeII RGCGCY 1 cut(s) 82
HaeIII GGCC 4 cut(s) 360, 699, 1343, 1376
HapII CCGG 3 cut(s) 309, 786, 1124
HgaI GACGC 1 cut(s) 757
HhaI GCGC 1 cut(s) 81
Hin1I GRCGYC 1 cut(s) 768
Hin1II CATG 6 cut(s) 383, 604, 705, 747, 1106, 1382
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 9 cut(s) 31, 182, 258, 907, 1012, 1049, 1187, 1247, 1295
HpaII CCGG 3 cut(s) 309, 786, 1124
HphI GGTGA 3 cut(s) 198, 854, 953
Hpy166II GTNNAC 2 cut(s) 60, 996
Hpy188I TCNGA 6 cut(s) 213, 257, 305, 760, 856, 1302
Hpy188III TCNNGA 9 cut(s) 8, 197, 380, 547, 885, 974, 1053, 1124, 1177
Hpy8I GTNNAC 2 cut(s) 60, 996
Hpy99I CGWCG 2 cut(s) 292, 1342
HpyAV CCTTC 6 cut(s) 262, 422, 659, 909, 1278, 1361
HpyCH4III ACNGT 6 cut(s) 14, 142, 194, 230, 679, 764
HpyCH4IV ACGT 3 cut(s) 523, 1337, 1395
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 231, 331, 467, 711, 774, 815
HpyF3I CTNAG 8 cut(s) 63, 88, 102, 210, 853, 960, 999, 1299
HpySE526I ACGT 3 cut(s) 523, 1337, 1395
Hsp92I GRCGYC 1 cut(s) 768
Hsp92II CATG 6 cut(s) 383, 604, 705, 747, 1106, 1382
HspAI GCGC 1 cut(s) 79
Kpn2I TCCGGA 1 cut(s) 1123
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 5 cut(s) 4, 376, 455, 1110, 1387
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 5 cut(s) 111, 249, 305, 592, 1179
Lsp1109I GCAGC 6 cut(s) 136, 356, 448, 637, 682, 976
LweI GCATC 5 cut(s) 136, 562, 852, 865, 1243
MaeI CTAG 4 cut(s) 27, 591, 773, 812
MaeII ACGT 3 cut(s) 523, 1337, 1395
MaeIII GTNAC 5 cut(s) 188, 194, 204, 596, 1017
MalI GATC 5 cut(s) 6, 378, 457, 1112, 1389
MbiI CCGCTC 2 cut(s) 1092, 1174
MboI GATC 5 cut(s) 4, 376, 455, 1110, 1387
MboII GAAGA 4 cut(s) 26, 1200, 1241, 1310
MflI RGATCY 3 cut(s) 4, 455, 1387
MhlI GDGCHC 3 cut(s) 323, 396, 1378
MluCI AATT 6 cut(s) 685, 837, 897, 977, 1140, 1156
MlyI GAGTC 5 cut(s) 267, 1006, 1043, 1196, 1241
MmeI TCCRAC 3 cut(s) 64, 283, 746
MnlI CCTC 7 cut(s) 104, 171, 219, 248, 305, 645, 1296
Mph1103I ATGCAT 1 cut(s) 880
MroI TCCGGA 1 cut(s) 1123
MseI TTAA 7 cut(s) 117, 281, 483, 822, 903, 1031, 1163
MslI CAYNNNNRTG 1 cut(s) 925
MspI CCGG 3 cut(s) 309, 786, 1124
MspR9I CCNGG 1 cut(s) 786
MwoI GCNNNNNNNGC 7 cut(s) 98, 231, 331, 467, 711, 774, 815
NciI CCSGG 1 cut(s) 786
NdeII GATC 5 cut(s) 4, 376, 455, 1110, 1387
NlaIII CATG 6 cut(s) 383, 604, 705, 747, 1106, 1382
NlaIV GGNNCC 4 cut(s) 6, 307, 1376, 1389
NmuCI GTSAC 2 cut(s) 194, 204
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 2 cut(s) 747, 1106
PagI TCATGA 1 cut(s) 379
PciI ACATGT 1 cut(s) 1102
PciSI GCTCTTC 1 cut(s) 1224
PfeI GAWTC 4 cut(s) 31, 182, 907, 1295
PflMI CCANNNNNTGG 1 cut(s) 1384
PkrI GCNGC 7 cut(s) 126, 346, 362, 463, 652, 672, 966
PleI GAGTC 5 cut(s) 266, 1006, 1043, 1195, 1241
PpsI GAGTC 5 cut(s) 266, 1006, 1043, 1195, 1241
Ppu21I YACGTR 1 cut(s) 524
PscI ACATGT 1 cut(s) 1102
Psp124BI GAGCTC 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 6, 307, 1376, 1389
PspOMI GGGCCC 1 cut(s) 1374
PspPI GGNCC 3 cut(s) 698, 1374, 1375
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 3 cut(s) 4, 455, 1387
RsaI GTAC 3 cut(s) 526, 1073, 1243
RsaNI GTAC 3 cut(s) 525, 1072, 1242
RseI CAYNNNNRTG 1 cut(s) 925
SacI GAGCTC 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 1224
SaqAI TTAA 7 cut(s) 117, 281, 483, 822, 903, 1031, 1163
SatI GCNGC 7 cut(s) 125, 345, 361, 462, 651, 671, 965
Sau3AI GATC 5 cut(s) 4, 376, 455, 1110, 1387
Sau96I GGNCC 3 cut(s) 698, 1374, 1375
SchI GAGTC 5 cut(s) 267, 1006, 1043, 1196, 1241
ScrFI CCNGG 1 cut(s) 786
SduI GDGCHC 3 cut(s) 323, 396, 1378
SfaNI GCATC 5 cut(s) 136, 562, 852, 865, 1243
SfcI CTRYAG 2 cut(s) 122, 226
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 1 cut(s) 547
SmoI CTYRAG 1 cut(s) 547
SnaBI TACGTA 1 cut(s) 524
Sse9I AATT 6 cut(s) 685, 837, 897, 977, 1140, 1156
SsiI CCGC 5 cut(s) 223, 361, 731, 1092, 1172
SspMI CTAG 4 cut(s) 27, 591, 773, 812
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 784
StyI CCWWGG 2 cut(s) 262, 772
TaaI ACNGT 6 cut(s) 14, 142, 194, 230, 679, 764
TaiI ACGT 3 cut(s) 526, 1340, 1398
TaqI TCGA 4 cut(s) 45, 96, 479, 1113
TaqII GACCGA 1 cut(s) 83
TasI AATT 6 cut(s) 685, 837, 897, 977, 1140, 1156
TatI WGTACW 1 cut(s) 1071
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 4 cut(s) 31, 182, 907, 1295
Tru1I TTAA 7 cut(s) 117, 281, 483, 822, 903, 1031, 1163
Tru9I TTAA 7 cut(s) 117, 281, 483, 822, 903, 1031, 1163
TscAI CASTG 3 cut(s) 430, 769, 1014
TseFI GTSAC 2 cut(s) 194, 204
TseI GCWGC 6 cut(s) 124, 344, 461, 650, 670, 964
Tsp45I GTSAC 2 cut(s) 194, 204
TspGWI ACGGA 3 cut(s) 166, 904, 1005
TspRI CASTG 3 cut(s) 430, 769, 1014
Van91I CCANNNNNTGG 1 cut(s) 1384
XapI RAATTY 1 cut(s) 977
XceI RCATGY 2 cut(s) 747, 1106
XmaJI CCTAGG 1 cut(s) 772
XspI CTAG 4 cut(s) 27, 591, 773, 812
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.