pycom03g14720

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012535
Physical Location & Seq
Reverse (-)
16022758 .. 16026082
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g14720.6

Sequence Viewer

Length: 1008 bp
ATGCACAGACAGATTATCCATACTGAAGGTTTTATTGGTCAGCCAAAAGTGAAATCTGCTGCAGCGGCTTGTCATTCGATTAACTCCACTATTCAGGTTGTGGAGTACCAAGAACCATTACGTACATCCAATGCTTTGGAAATTATGAGCAAATATGATATAGTTGTAGATGCAACGGACAATGCTCCTAGTCGTTACATGATTAGCGATTGTTGTGTGGTGATAGGGAAGCCTCTTGTATCTGGTGCTGCAGTGGGATTAGAAGGGCAGCTCACAGTTTTCAATTATAATGGTGGTCCGTGCTATCGATGCCTATTTCCAACTCCGCCACCTACAGCCGCATGCCAAAGATGTTCTGACGCTGGTGTCCTAGGCATTGTTCCAGGTGTCATCGGCTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAAGTGGAGTTGGCGAACCACTCTCAGAACGCATGCTTTTATTTGATGCATTTTCAGGACGGATACGAAATGTTAAGATTCGAGGAAGGTCACTGCAGTGCGTAGTGTGTGGTGAGAATGCACCATTCTCTAAGCAGCAGTTTCAAGAATTTGATTATGAAAACTTCACTCAGTCTCCACTGACTCCGTTACCTTTGAAGTTAAACCTACTTCAGCCAGACTCCAGGATAAATAGTAAAGAGTACAAGGAGAAACTAGTTAGTGGCGAGGCACATGTTTTGGTCGACGTTCGTCCAGAACATCACTTCAAGATTGTTTCTCTGCCCAATTCCTTAAACATCCCGCTCCCACGTTTGGAAGCTCGGTTACCAGAAATAACTTCAGCCTTAAAGGAAAAGGAAGACCATAGGGGTACTGATTCTGGTTCAGGTGGGCAAGTATATGTAATATGTAGAAGGGGTAACGATTCTCAAAGGGCTGTTCAGTATCTCCACAAAATGGGTTTCACATCGGCCAAAGACATCATCGGAGGACTGGAGGGCTGGGCGCATGAGGTCGATCCAAACGTCCCTACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

336

Amino Acids

36.66

Weight (kDa)

6.75

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AasI GACNNNNNNGTC 1 cut(s) 365
AccB7I CCANNNNNTGG 1 cut(s) 925
AccBSI CCGCTC 1 cut(s) 772
AccI GTMKAC 1 cut(s) 711
AciI CCGC 4 cut(s) 65, 326, 339, 770
AclWI GGATC 1 cut(s) 980
AcoI YGGCCR 1 cut(s) 939
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 3 cut(s) 45, 623, 792
AfaI GTAC 4 cut(s) 107, 124, 671, 841
AfiI CCNNNNNNNGG 2 cut(s) 781, 925
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 4 cut(s) 283, 572, 625, 736
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 2 cut(s) 382, 650
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
AleI CACNNNNGTG 1 cut(s) 523
AluBI AGCT 3 cut(s) 271, 416, 788
AluI AGCT 3 cut(s) 271, 416, 788
Alw26I GTCTC 2 cut(s) 404, 606
AlwI GGATC 1 cut(s) 980
AoxI GGCC 1 cut(s) 939
ApeKI GCWGC 5 cut(s) 59, 62, 248, 268, 562
ApoI RAATTY 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 370
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 232, 551
AvaII GGWCC 1 cut(s) 296
AvrII CCTAGG 1 cut(s) 370
BarI GAAGNNNNNNTAC 2 cut(s) 777, 809
BbsI GAAGAC 1 cut(s) 834
BbvI GCAGC 5 cut(s) 46, 74, 235, 280, 574
BciT130I CCWGG 2 cut(s) 384, 652
BciVI GTATCC 1 cut(s) 483
BcoDI GTCTC 2 cut(s) 404, 606
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 4 cut(s) 189, 371, 410, 683
BfmI CTRYAG 4 cut(s) 60, 249, 333, 521
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 7 cut(s) 60, 63, 66, 249, 269, 339, 563
BlnI CCTAGG 1 cut(s) 370
BlsI GCNGC 7 cut(s) 61, 64, 67, 250, 270, 340, 564
Bme1390I CCNGG 2 cut(s) 384, 652
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmrFI CCNGG 2 cut(s) 384, 652
BmsI GCATC 3 cut(s) 160, 299, 463
BoxI GACNNNNGTC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 2 cut(s) 634, 983
Bsa29I ATCGAT 1 cut(s) 307
BsaAI YACGTR 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 586, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 781, 925
Bse1I ACTGG 1 cut(s) 966
BseBI CCWGG 2 cut(s) 384, 652
BseCI ATCGAT 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 2 cut(s) 125, 765
BseLI CCNNNNNNNGG 2 cut(s) 781, 925
BseMII CTCAG 2 cut(s) 465, 611
BseNI ACTGG 1 cut(s) 966
BseXI GCAGC 5 cut(s) 46, 74, 235, 280, 574
BseYI CCCAGC 1 cut(s) 969
BshFI GGCC 1 cut(s) 941
BshVI ATCGAT 1 cut(s) 307
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 2 cut(s) 781, 925
BsmAI GTCTC 2 cut(s) 404, 606
BsmFI GGGAC 1 cut(s) 980
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 1 cut(s) 941
Bsp143I GATC 1 cut(s) 985
BspACI CCGC 4 cut(s) 65, 326, 339, 770
BspANI GGCC 1 cut(s) 941
BspCNI CTCAG 2 cut(s) 464, 610
BspDI ATCGAT 1 cut(s) 307
BspMAI CTGCAG 3 cut(s) 64, 253, 525
BspPI GGATC 1 cut(s) 980
BsrBI CCGCTC 1 cut(s) 772
BsrI ACTGG 1 cut(s) 966
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 1 cut(s) 985
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 2 cut(s) 384, 652
Bst4CI ACNGT 1 cut(s) 277
BstBAI YACGTR 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 343, 461
BstDEI CTNAG 3 cut(s) 451, 558, 597
BstEII GGTNACC 1 cut(s) 792
BstF5I GGATG 2 cut(s) 125, 765
BstHHI GCGC 1 cut(s) 976
BstKTI GATC 1 cut(s) 988
BstMAI GTCTC 2 cut(s) 404, 606
BstMBI GATC 1 cut(s) 985
BstMWI GCNNNNNNNGC 3 cut(s) 65, 309, 413
BstNI CCWGG 2 cut(s) 384, 652
BstNSI RCATGY 3 cut(s) 345, 463, 704
BstPAI GACNNNNGTC 1 cut(s) 717
BstPI GGTNACC 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 382, 650
BstSFI CTRYAG 4 cut(s) 60, 249, 333, 521
BstSNI TACGTA 1 cut(s) 122
BstV1I GCAGC 5 cut(s) 46, 74, 235, 280, 574
BstV2I GAAGAC 1 cut(s) 834
BstXI CCANNNNNNTGG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 307
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 1 cut(s) 941
BsuTUI ATCGAT 1 cut(s) 307
BtsCI GGATG 2 cut(s) 125, 765
BtsI GCAGTG 3 cut(s) 258, 518, 530
BtsIMutI CAGTG 4 cut(s) 258, 518, 530, 605
Cac8I GCNNGC 2 cut(s) 343, 461
CfoI GCGC 1 cut(s) 976
Cfr13I GGNCC 1 cut(s) 296
ClaI ATCGAT 1 cut(s) 307
CseI GACGC 1 cut(s) 368
Csp6I GTAC 4 cut(s) 106, 123, 670, 840
CviAII CATG 5 cut(s) 199, 342, 460, 701, 977
CviQI GTAC 4 cut(s) 106, 123, 670, 840
DdeI CTNAG 3 cut(s) 451, 558, 597
DpnI GATC 1 cut(s) 987
DpnII GATC 1 cut(s) 985
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 939
EciI GGCGGA 1 cut(s) 315
Eco105I TACGTA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 3 cut(s) 45, 623, 792
Eco91I GGTNACC 1 cut(s) 792
EcoO65I GGTNACC 1 cut(s) 792
EcoRII CCWGG 2 cut(s) 382, 650
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 5 cut(s) 202, 345, 463, 704, 980
FaqI GGGAC 1 cut(s) 980
FatI CATG 5 cut(s) 198, 341, 459, 700, 976
FauI CCCGC 1 cut(s) 777
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 7 cut(s) 60, 63, 66, 249, 269, 339, 563
FokI GGATG 2 cut(s) 112, 752
Fsp4HI GCNGC 7 cut(s) 60, 63, 66, 249, 269, 339, 563
FspBI CTAG 4 cut(s) 189, 371, 410, 683
GlaI GCGC 1 cut(s) 975
GluI GCNGC 7 cut(s) 60, 63, 66, 249, 269, 339, 563
GsaI CCCAGC 1 cut(s) 973
GsuI CTGGAG 2 cut(s) 634, 983
HaeIII GGCC 1 cut(s) 941
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 976
Hin1II CATG 5 cut(s) 202, 345, 463, 704, 980
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HinfI GANTC 5 cut(s) 505, 610, 647, 845, 893
HphI GGTGA 2 cut(s) 232, 551
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 3 cut(s) 358, 454, 956
Hpy188III TCNNGA 4 cut(s) 483, 572, 722, 736
Hpy8I GTNNAC 1 cut(s) 712
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 4 cut(s) 20, 257, 507, 876
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 4 cut(s) 121, 714, 778, 993
HpyCH4V TGCA 8 cut(s) 4, 62, 173, 251, 428, 476, 523, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 309, 413
HpyF3I CTNAG 3 cut(s) 451, 558, 597
HpySE526I ACGT 4 cut(s) 121, 714, 778, 993
Hsp92II CATG 5 cut(s) 202, 345, 463, 704, 980
HspAI GCGC 1 cut(s) 974
Kzo9I GATC 1 cut(s) 985
LmnI GCTCC 2 cut(s) 190, 777
Lsp1109I GCAGC 5 cut(s) 46, 74, 235, 280, 574
LweI GCATC 3 cut(s) 160, 299, 463
MaeI CTAG 4 cut(s) 189, 371, 410, 683
MaeII ACGT 4 cut(s) 121, 714, 778, 993
MaeIII GTNAC 5 cut(s) 194, 516, 615, 792, 887
MalI GATC 1 cut(s) 987
MbiI CCGCTC 1 cut(s) 772
MboI GATC 1 cut(s) 985
MboII GAAGA 1 cut(s) 839
MluCI AATT 4 cut(s) 141, 283, 575, 754
MlyI GAGTC 2 cut(s) 604, 641
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 6 cut(s) 243, 503, 688, 950, 958, 973
Mph1103I ATGCAT 1 cut(s) 478
MseI TTAA 6 cut(s) 81, 420, 501, 629, 761, 815
MslI CAYNNNNRTG 1 cut(s) 523
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 384, 652
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 2 cut(s) 384, 652
MwoI GCNNNNNNNGC 3 cut(s) 65, 309, 413
NdeII GATC 1 cut(s) 985
NlaIII CATG 5 cut(s) 202, 345, 463, 704, 980
NmuCI GTSAC 1 cut(s) 516
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 3 cut(s) 345, 463, 704
OliI CACNNNNGTG 1 cut(s) 523
PaeI GCATGC 2 cut(s) 345, 463
PciI ACATGT 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 990
PctI GAATGC 1 cut(s) 550
PfeI GAWTC 3 cut(s) 505, 845, 893
PflMI CCANNNNNTGG 1 cut(s) 925
PfoI TCCNGGA 1 cut(s) 650
PkrI GCNGC 7 cut(s) 61, 64, 67, 250, 270, 340, 564
PleI GAGTC 2 cut(s) 604, 641
PpsI GAGTC 2 cut(s) 604, 641
Ppu21I YACGTR 1 cut(s) 122
PscI ACATGT 1 cut(s) 700
PshAI GACNNNNGTC 1 cut(s) 717
PsiI TTATAA 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 382, 650
PspEI GGTNACC 1 cut(s) 792
PspFI CCCAGC 1 cut(s) 969
PspGI CCWGG 2 cut(s) 382, 650
PspPI GGNCC 1 cut(s) 296
PstI CTGCAG 3 cut(s) 64, 253, 525
RsaI GTAC 4 cut(s) 107, 124, 671, 841
RsaNI GTAC 4 cut(s) 106, 123, 670, 840
RseI CAYNNNNRTG 1 cut(s) 523
SalI GTCGAC 1 cut(s) 710
SaqAI TTAA 6 cut(s) 81, 420, 501, 629, 761, 815
SatI GCNGC 7 cut(s) 60, 63, 66, 249, 269, 339, 563
Sau3AI GATC 1 cut(s) 985
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 2 cut(s) 604, 641
ScrFI CCNGG 2 cut(s) 384, 652
SfaNI GCATC 3 cut(s) 160, 299, 463
SfcI CTRYAG 4 cut(s) 60, 249, 333, 521
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 523
SnaBI TACGTA 1 cut(s) 122
SpeI ACTAGT 1 cut(s) 682
SphI GCATGC 2 cut(s) 345, 463
Sse9I AATT 4 cut(s) 141, 283, 575, 754
SsiI CCGC 4 cut(s) 65, 326, 339, 770
SspMI CTAG 4 cut(s) 189, 371, 410, 683
StyD4I CCNGG 2 cut(s) 382, 650
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 4 cut(s) 124, 717, 781, 996
TaqI TCGA 5 cut(s) 77, 307, 508, 711, 984
TasI AATT 4 cut(s) 141, 283, 575, 754
TatI WGTACW 1 cut(s) 669
TauI GCSGC 2 cut(s) 68, 341
TfiI GAWTC 3 cut(s) 505, 845, 893
Tru1I TTAA 6 cut(s) 81, 420, 501, 629, 761, 815
Tru9I TTAA 6 cut(s) 81, 420, 501, 629, 761, 815
TscAI CASTG 4 cut(s) 258, 525, 530, 612
TseFI GTSAC 1 cut(s) 516
TseI GCWGC 5 cut(s) 59, 62, 248, 268, 562
Tsp45I GTSAC 1 cut(s) 516
TspDTI ATGAA 1 cut(s) 600
TspGWI ACGGA 4 cut(s) 191, 288, 502, 603
TspRI CASTG 4 cut(s) 258, 525, 530, 612
Van91I CCANNNNNTGG 1 cut(s) 925
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 575
XceI RCATGY 3 cut(s) 345, 463, 704
XmaJI CCTAGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 4 cut(s) 189, 371, 410, 683
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.