Rh5BG280700

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012535
Physical Location & Seq
Forward (+)
35440057 .. 35444130
4074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG280700.1

Sequence Viewer

Length: 1401 bp
ATGGCCGCCGCCGACGGTGGAGAAGTGACTGCAATTCTCCGCGACCTCGAAAGCTTGAAGGCGAGTAAGAGCGAAATCGAGCTCCGAATCTCGGCCCTCGAAGCTCGCCTTCAAGAGATTAATCTTCAGCAGCCGAACGGTGCCGTTTCGAATGGCTCTTGTGCTCCTCTGCCGTCGTCCGTTGATTCCGGCTACGGTCACGATTTGTCGCCTCAGATGATTTACCGCTACAGTCGCCAGCTCTTGCTTCCCTCTTTCGGAGTCCAAGCTCAGTCGAAGCTCCTAAAGTCATCGATTTTGGTGGTTGGAGCTGGAGGGTTGGGCTCGCCGGCTCTGCTATATCTTGCAGCTTGTGGTGTTGGCCACTTGGGGATTATTGATCATGATGTGGTTGAGCTCAACAATATGCACAGGCAGATTATCCACACTGAAAGATTTATTGGTCAGCCCAAAGTGAGATCTGCTGCAGCTGCTTGTCGATCGATTAACTCCACTATCCAGGTTGTGGAATACCAAGAACCATTACGCACATCCAATGCTTTGGAAATCATGAGCAAATATGATATAATAGTGGATGCCACGGACAATGCTCCTAGCCGTTACATGATAAGCGATTGCTGTGTGGTGCTAGGGAAGCCTCTTGTATCTGGTGCCGCACTCGGATTTGAAGGGCAGCTCACGGTGTACAATTACAATGGTGGTCCATGCTATCGATGCCTATTTCCAACTCCTCCACCTACAACAGCATGTCAAAGGTGTTCTGATAGTGGTGTACTAGGTGTAGTTCCAGGTATCATTGGTTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAGGTGAAGTTGGTGAACCACTCTCAGGACGAATGCTTTTACTTGATTCATTGTCAGGACGGATTCGAATTGTTAAGATCCGAGGAAGGTCCTTGCAATGCGTAGCTTGTGGTGAAAATACAACATTCTCTAAGCAGCAATTTCGGGAATTTGATTATGAAAACTTCACTCAGTCTCCACTAACTCCGTCACCTTTGAAGTTAAACCTACTTCAGGCAGACTCCAGAATAAATACTAAAGAGTACAAGGAGAAAATTGATGACGGGGAAGCACATGTTTTGATTGATGTTCGTCCAGAACACCACTTCAAGATCGTTTCTCTACCCAATTCATTAAACATTCCACTCCCAAGTTTGGAGGCTCGGTTACCTGAAATATCTCTAGCTTTAAAGGAAAACGAAGAGAATAGAGGTGTTAATTCTGAACTATATGTAGTATGTAGAAGGGGTAATGATTCTCAGAGGGCTGTTCAGAGTCTCCACAAGCTGGGTTTCACCTCAGCCAGAGACATCATTGGGGGACTGGAGGCCTGGGCTCGTGATGTGGATCCAAACTTTCCTACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

466

Amino Acids

50.64

Weight (kDa)

6.04

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 77 - 311 4.2e-82 ThiF family
Rhodanese PF00581 356 - 457 1.1e-14 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 824
Acc36I ACCTGC 1 cut(s) 824
AccB1I GGYRCC 2 cut(s) 140, 650
AccB7I CCANNNNNTGG 2 cut(s) 505, 1318
AccII CGCG 1 cut(s) 42
AciI CCGC 5 cut(s) 6, 9, 40, 226, 654
AclWI GGATC 3 cut(s) 904, 1373, 1386
AcoI YGGCCR 2 cut(s) 3, 361
AcsI RAATTY 1 cut(s) 980
AcuI CTGAAG 2 cut(s) 110, 1028
AfaI GTAC 3 cut(s) 686, 774, 1076
AfiI CCNNNNNNNGG 7 cut(s) 91, 257, 505, 857, 1045, 1186, 1318
AflIII ACRYGT 1 cut(s) 1105
AgsI TTSAA 5 cut(s) 58, 113, 668, 1030, 1141
AjnI CCWGG 3 cut(s) 498, 787, 1361
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
Alw21I GWGCWC 3 cut(s) 84, 166, 399
Alw26I GTCTC 4 cut(s) 809, 1011, 1313, 1332
AlwI GGATC 3 cut(s) 904, 1373, 1386
AoxI GGCC 4 cut(s) 3, 93, 361, 1359
ApeKI GCWGC 7 cut(s) 130, 347, 464, 467, 470, 673, 967
ApoI RAATTY 1 cut(s) 980
AseI ATTAAT 1 cut(s) 120
AspS9I GGNCC 3 cut(s) 94, 701, 921
AsuHPI GGTGA 5 cut(s) 848, 857, 956, 1014, 1318
AsuII TTCGAA 2 cut(s) 149, 898
AvaII GGWCC 2 cut(s) 701, 921
BalI TGGCCA 1 cut(s) 363
BamHI GGATCC 1 cut(s) 1378
BanI GGYRCC 2 cut(s) 140, 650
BanII GRGCYC 4 cut(s) 84, 326, 399, 1369
BauI CACGAG 1 cut(s) 1368
Bbv12I GWGCWC 3 cut(s) 84, 166, 399
BbvCI CCTCAGC 1 cut(s) 1330
BbvI GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
BceAI ACGGC 3 cut(s) 128, 157, 582
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 3 cut(s) 500, 789, 1363
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 4 cut(s) 809, 1011, 1313, 1332
BfaI CTAG 5 cut(s) 594, 629, 776, 815, 1214
BfmI CTRYAG 2 cut(s) 229, 465
BfuAI ACCTGC 1 cut(s) 824
BglII AGATCT 1 cut(s) 458
Bme1390I CCNGG 3 cut(s) 500, 789, 1363
Bme18I GGWCC 2 cut(s) 701, 921
BmgT120I GGNCC 3 cut(s) 94, 701, 921
BmiI GGNNCC 3 cut(s) 142, 652, 1380
BmrFI CCNGG 3 cut(s) 500, 789, 1363
BmsI GCATC 2 cut(s) 565, 704
BpmI CTGGAG 3 cut(s) 333, 1039, 1376
Bpu10I CCTNAGC 1 cut(s) 1330
Bpu14I TTCGAA 2 cut(s) 149, 898
Bsa29I ATCGAT 3 cut(s) 293, 482, 712
BsaBI GATNNNNATC 1 cut(s) 1377
BsaJI CCNNGG 3 cut(s) 579, 913, 1362
BsaXI ACNNNNNCTCC 4 cut(s) 991, 1021, 1181, 1211
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 257, 505, 857, 1045, 1186, 1318
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 1359
Bse3DI GCAATG 1 cut(s) 935
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 3 cut(s) 500, 789, 1363
BseCI ATCGAT 3 cut(s) 293, 482, 712
BseDI CCNNGG 3 cut(s) 579, 913, 1362
BseGI GGATG 2 cut(s) 530, 580
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 7 cut(s) 91, 257, 505, 857, 1045, 1186, 1318
BseMI GCAATG 1 cut(s) 935
BseMII CTCAG 6 cut(s) 227, 284, 870, 1016, 1304, 1344
BseNI ACTGG 1 cut(s) 1359
BseRI GAGGAG 2 cut(s) 156, 720
BseXI GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
BseYI CCCAGC 1 cut(s) 1318
Bsh1236I CGCG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 4 cut(s) 5, 95, 363, 1361
BshNI GGYRCC 2 cut(s) 140, 650
BshVI ATCGAT 3 cut(s) 293, 482, 712
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 3 cut(s) 84, 166, 399
BsiSI CCGG 2 cut(s) 189, 329
BslFI GGGAC 1 cut(s) 1365
BslI CCNNNNNNNGG 7 cut(s) 91, 257, 505, 857, 1045, 1186, 1318
BsmAI GTCTC 4 cut(s) 809, 1011, 1313, 1332
BsmFI GGGAC 1 cut(s) 1365
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 4 cut(s) 5, 95, 363, 1361
Bsp119I TTCGAA 2 cut(s) 149, 898
Bsp1286I GDGCHC 5 cut(s) 84, 166, 326, 399, 1369
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
BspACI CCGC 5 cut(s) 6, 9, 40, 226, 654
BspANI GGCC 4 cut(s) 5, 95, 363, 1361
BspCNI CTCAG 6 cut(s) 226, 283, 869, 1015, 1303, 1343
BspDI ATCGAT 3 cut(s) 293, 482, 712
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 2 cut(s) 382, 549
BspLI GGNNCC 3 cut(s) 142, 652, 1380
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 3 cut(s) 904, 1373, 1386
BspT104I TTCGAA 2 cut(s) 149, 898
BspT107I GGYRCC 2 cut(s) 140, 650
BsrDI GCAATG 1 cut(s) 935
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1359
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 579, 913, 1362
BssMI GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
BssSI CACGAG 1 cut(s) 1368
Bst2BI CACGAG 1 cut(s) 1368
Bst2UI CCWGG 3 cut(s) 500, 789, 1363
Bst4CI ACNGT 5 cut(s) 17, 140, 197, 233, 682
Bst6I CTCTTC 1 cut(s) 1227
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 2 cut(s) 149, 898
BstC8I GCNNGC 4 cut(s) 106, 239, 326, 330
BstDEI CTNAG 7 cut(s) 213, 270, 856, 963, 1002, 1290, 1330
BstDSI CCRYGG 1 cut(s) 579
BstEII GGTNACC 1 cut(s) 1197
BstENI CCTNNNNNAGG 1 cut(s) 1043
BstF5I GGATG 2 cut(s) 530, 580
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 6 cut(s) 382, 461, 482, 912, 1146, 1381
BstMAI GTCTC 4 cut(s) 809, 1011, 1313, 1332
BstMBI GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
BstMCI CGRYCG 1 cut(s) 482
BstMWI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 818
BstNI CCWGG 3 cut(s) 500, 789, 1363
BstNSI RCATGY 2 cut(s) 750, 1109
BstPI GGTNACC 1 cut(s) 1197
BstSCI CCNGG 3 cut(s) 498, 787, 1361
BstSFI CTRYAG 2 cut(s) 229, 465
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
BstX2I RGATCY 3 cut(s) 458, 909, 1378
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 3 cut(s) 458, 909, 1378
Bsu15I ATCGAT 3 cut(s) 293, 482, 712
BsuRI GGCC 4 cut(s) 5, 95, 363, 1361
BsuTUI ATCGAT 3 cut(s) 293, 482, 712
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 2 cut(s) 530, 580
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 4 cut(s) 106, 239, 326, 330
CciI TCATGA 2 cut(s) 382, 549
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 94, 701, 921
ClaI ATCGAT 3 cut(s) 293, 482, 712
Csp6I GTAC 3 cut(s) 685, 773, 1075
CviAII CATG 6 cut(s) 383, 550, 604, 705, 747, 1106
CviQI GTAC 3 cut(s) 685, 773, 1075
DdeI CTNAG 7 cut(s) 213, 270, 856, 963, 1002, 1290, 1330
DpnI GATC 6 cut(s) 381, 460, 481, 911, 1145, 1380
DpnII GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
DraI TTTAAA 1 cut(s) 1221
EaeI YGGCCR 2 cut(s) 3, 361
Eam1104I CTCTTC 1 cut(s) 1227
EarI CTCTTC 1 cut(s) 1227
Ecl136II GAGCTC 2 cut(s) 82, 397
Eco147I AGGCCT 1 cut(s) 1361
Eco24I GRGCYC 4 cut(s) 84, 326, 399, 1369
Eco47I GGWCC 2 cut(s) 701, 921
Eco53kI GAGCTC 2 cut(s) 82, 397
Eco57I CTGAAG 2 cut(s) 110, 1028
Eco91I GGTNACC 1 cut(s) 1197
EcoICRI GAGCTC 2 cut(s) 82, 397
EcoNI CCTNNNNNAGG 1 cut(s) 1043
EcoO109I RGGNCCY 1 cut(s) 921
EcoO65I GGTNACC 1 cut(s) 1197
EcoRII CCWGG 3 cut(s) 498, 787, 1361
EcoT38I GRGCYC 4 cut(s) 84, 326, 399, 1369
FaeI CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 1 cut(s) 1365
FatI CATG 6 cut(s) 382, 549, 603, 704, 746, 1105
FbaI TGATCA 1 cut(s) 379
FokI GGATG 2 cut(s) 517, 587
FriOI GRGCYC 4 cut(s) 84, 326, 399, 1369
FspBI CTAG 5 cut(s) 594, 629, 776, 815, 1214
GsaI CCCAGC 1 cut(s) 1322
GsuI CTGGAG 3 cut(s) 333, 1039, 1376
HaeIII GGCC 4 cut(s) 5, 95, 363, 1361
HapII CCGG 2 cut(s) 189, 329
Hin1II CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 8 cut(s) 87, 185, 261, 878, 895, 1052, 1286, 1306
HpaII CCGG 2 cut(s) 189, 329
HphI GGTGA 5 cut(s) 848, 857, 956, 1014, 1318
Hpy166II GTNNAC 3 cut(s) 685, 773, 848
Hpy188I TCNGA 9 cut(s) 86, 216, 260, 662, 763, 914, 1255, 1293, 1305
Hpy8I GTNNAC 3 cut(s) 685, 773, 848
Hpy99I CGWCG 2 cut(s) 17, 178
HpyAV CCTTC 5 cut(s) 52, 119, 662, 912, 1269
HpyCH4III ACNGT 5 cut(s) 17, 140, 197, 233, 682
HpyCH4V TGCA 6 cut(s) 32, 347, 409, 467, 833, 928
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 818
HpyF3I CTNAG 7 cut(s) 213, 270, 856, 963, 1002, 1290, 1330
Hsp92II CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
LmnI GCTCC 5 cut(s) 87, 169, 285, 308, 595
Lsp1109I GCAGC 7 cut(s) 142, 359, 451, 457, 479, 685, 979
LweI GCATC 2 cut(s) 565, 704
MaeI CTAG 5 cut(s) 594, 629, 776, 815, 1214
MaeIII GTNAC 5 cut(s) 25, 197, 599, 1020, 1197
MalI GATC 6 cut(s) 381, 460, 481, 911, 1145, 1380
MboI GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
MboII GAAGA 2 cut(s) 116, 1244
MflI RGATCY 3 cut(s) 458, 909, 1378
MhlI GDGCHC 5 cut(s) 84, 166, 326, 399, 1369
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 8 cut(s) 33, 688, 900, 971, 980, 1086, 1159, 1249
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 3 cut(s) 270, 1046, 1315
MmeI TCCRAC 2 cut(s) 286, 749
Mox20I TGGCCA 1 cut(s) 363
MroNI GCCGGC 1 cut(s) 328
MscI TGGCCA 1 cut(s) 363
MseI TTAA 8 cut(s) 120, 486, 825, 906, 1034, 1166, 1220, 1248
Msp20I TGGCCA 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 189, 329
MspR9I CCNGG 3 cut(s) 500, 789, 1363
Mva1269I GAATGC 1 cut(s) 870
MvaI CCWGG 3 cut(s) 500, 789, 1363
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 7 cut(s) 101, 234, 334, 470, 634, 714, 818
NaeI GCCGGC 1 cut(s) 330
NdeII GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 6 cut(s) 386, 553, 607, 708, 750, 1109
NlaIV GGNNCC 3 cut(s) 142, 652, 1380
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 3 cut(s) 25, 197, 1020
NspI RCATGY 2 cut(s) 750, 1109
NspV TTCGAA 2 cut(s) 149, 898
PagI TCATGA 2 cut(s) 382, 549
PaqCI CACCTGC 1 cut(s) 824
PceI AGGCCT 1 cut(s) 1361
PciI ACATGT 1 cut(s) 1105
PctI GAATGC 1 cut(s) 870
PdiI GCCGGC 1 cut(s) 330
PfeI GAWTC 5 cut(s) 87, 185, 878, 895, 1286
PflMI CCANNNNNTGG 2 cut(s) 505, 1318
Ple19I CGATCG 1 cut(s) 482
PleI GAGTC 3 cut(s) 269, 1046, 1314
PpsI GAGTC 3 cut(s) 269, 1046, 1314
PpuMI RGGWCCY 1 cut(s) 921
PscI ACATGT 1 cut(s) 1105
PshBI ATTAAT 1 cut(s) 120
Psp124BI GAGCTC 2 cut(s) 84, 399
Psp5II RGGWCCY 1 cut(s) 921
Psp6I CCWGG 3 cut(s) 498, 787, 1361
PspEI GGTNACC 1 cut(s) 1197
PspFI CCCAGC 1 cut(s) 1318
PspGI CCWGG 3 cut(s) 498, 787, 1361
PspN4I GGNNCC 3 cut(s) 142, 652, 1380
PspPI GGNCC 3 cut(s) 94, 701, 921
PspPPI RGGWCCY 1 cut(s) 921
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 3 cut(s) 458, 909, 1378
PvuI CGATCG 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 3 cut(s) 686, 774, 1076
RsaNI GTAC 3 cut(s) 685, 773, 1075
SacI GAGCTC 2 cut(s) 84, 399
SaqAI TTAA 8 cut(s) 120, 486, 825, 906, 1034, 1166, 1220, 1248
Sau3AI GATC 6 cut(s) 379, 458, 479, 909, 1143, 1378
Sau96I GGNCC 3 cut(s) 94, 701, 921
SchI GAGTC 3 cut(s) 270, 1046, 1315
ScrFI CCNGG 3 cut(s) 500, 789, 1363
SduI GDGCHC 5 cut(s) 84, 166, 326, 399, 1369
SfaNI GCATC 2 cut(s) 565, 704
SfcI CTRYAG 2 cut(s) 229, 465
SfuI TTCGAA 2 cut(s) 149, 898
SinI GGWCC 2 cut(s) 701, 921
Sse9I AATT 8 cut(s) 33, 688, 900, 971, 980, 1086, 1159, 1249
SseBI AGGCCT 1 cut(s) 1361
SsiI CCGC 5 cut(s) 6, 9, 40, 226, 654
SspMI CTAG 5 cut(s) 594, 629, 776, 815, 1214
SstI GAGCTC 2 cut(s) 84, 399
StuI AGGCCT 1 cut(s) 1361
StyD4I CCNGG 3 cut(s) 498, 787, 1361
TaaI ACNGT 5 cut(s) 17, 140, 197, 233, 682
TasI AATT 8 cut(s) 33, 688, 900, 971, 980, 1086, 1159, 1249
TatI WGTACW 3 cut(s) 684, 772, 1074
TauI GCSGC 3 cut(s) 8, 11, 656
TfiI GAWTC 5 cut(s) 87, 185, 878, 895, 1286
Tru1I TTAA 8 cut(s) 120, 486, 825, 906, 1034, 1166, 1220, 1248
Tru9I TTAA 8 cut(s) 120, 486, 825, 906, 1034, 1166, 1220, 1248
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 3 cut(s) 25, 197, 1020
TseI GCWGC 7 cut(s) 130, 347, 464, 467, 470, 673, 967
Tsp45I GTSAC 3 cut(s) 25, 197, 1020
TspDTI ATGAA 3 cut(s) 870, 1005, 1152
TspGWI ACGGA 4 cut(s) 169, 596, 907, 1008
TspRI CASTG 1 cut(s) 433
Van91I CCANNNNNTGG 2 cut(s) 505, 1318
VpaK11BI GGWCC 2 cut(s) 701, 921
VspI ATTAAT 1 cut(s) 120
XagI CCTNNNNNAGG 1 cut(s) 1043
XapI RAATTY 1 cut(s) 980
XceI RCATGY 2 cut(s) 750, 1109
XspI CTAG 5 cut(s) 594, 629, 776, 815, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.