MD03G1195100.v1.1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012535
Physical Location & Seq
Reverse (-)
26722699 .. 26727448
4750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1195100.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGACAAGATTGCCCCTTAAAAGTGAAACATCGACAAAACCCGCAGGGACTCGCGCGCAGCGACCGTCTCTTCCAAACCTCCAAAGCTCCCTGATCAGCTCAGCTTTTGCTCTCTGCGACTGCGACTCCATGGATTCCGATCGGGGCGGCGAGGCCTCTGTGATTCTTCGCCAACTCGAAGACTTGAAGGCCAGTAAGAGCGAAATAGAGCGCCAAATATCGGCCCTCGAAGCTCAGCTCCGAGCGATTACCATTCAGAAACCGAACGGCAGCGTTTCTAATGGCTCTTGTCCTCCCCATATACCGTCCGTTGATTCGGGCTATGGTCACGATTTGTCGCCTCAGATGATTCACCGCTACAGTCGCCACCTCTTGCTTCCTTCGTTCGGAGTCCAAGGGCAGTCAAAGCTCTTGAAGTCATCGATTTTGGTGGTCGGAGCTGGAGGGTTAGGCTCGCCTGTTCTGCTGTATCTTGCAGCCTGTGGTGTTGGCTGCTTGGGGATCGTTGATCATGATGTGGTTGAGCTCAATAATATGCACAGACAGATTATCCATGCTGAAGGTTTTATTGGTCAGCCAAAAGTGAAATCTGCTGCAGCGGCTTGTCATTCGATTAACTCCACTATTCAGGTTGTGGAGTACCAAGAACCATTACGTACATCCAATGCTTTGGAAATTATGAGCAAATATGATATAGTAGTAGATGCAACGGACAATGCTCCTAGTCGTTACATGATTAGCGATTGTTGTGTGGTGATAGGGAAGCCTCTTGTATCTGGTGCTGCAGTGGGATTAGAAGGGCAGCTCACAGTTTTCAATTATAATGGTGGTCCATGCTATCGATGCCTATTTCCAACTCCGCCACCTACAACTGCATGCCAAAGATGTTCTGATGCTGGTGTCCTAGGCGTTGTACCAGGTGTCATCGGCTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAAGTGGAGTTGGCGAACCACTCTCAGAACGCATGCTTTTATTTGATGCATTTTCAGGACGGATACGAAATGTTAAGATTCGAGGAAGGTCACCGCAGTGCGTAGTGTGTGGTGAGAATGCACCATTCTCTAAGCAGCAGTTTCAAGAATTTGATTATGAAAACTTCACTCAGTCTCCACTGACTCCGTTACCTTTGAAGTTAAACCTACTTCAGCCAGACGCCAGAATAAATAGTAAAGAGTACAAGGAGAAAATAATTAGTGGTGAGGCACATGTTTTAATCGACGTTCGTCCAGAACATCACTTCAAGATTGTTTCTCTGCCCAATTCCTTAAACATCCCGCTCCCACGTTTGGAGGCTCGGTTGCCGGAAATAACTTCAGCCTTAAAGGAAAAGGAAGACCATAGGGGTACTGATTCTGGTTCAGGTGGAAAGGTATATGTAATATGTAGAAGGGGTAACGATTCTCAAAGGGCTGTTCAGTATCTCCACAAGATGGGTTTCACATCGGCCAAAGACATCATCGGAGGACTGGAGGGCTGGGCGCATGAGGTCGATCCAAACGTCCCTACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

514

Amino Acids

55.44

Weight (kDa)

7.18

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 120 - 355 6.8e-83 ThiF family
Rhodanese PF00581 400 - 504 3e-13 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
AccB7I CCANNNNNTGG 1 cut(s) 1459
AccBSI CCGCTC 1 cut(s) 1306
AccII CGCG 2 cut(s) 54, 56
AciI CCGC 7 cut(s) 42, 147, 355, 599, 860, 1055, 1304
AclWI GGATC 2 cut(s) 509, 1514
AcoI YGGCCR 1 cut(s) 1473
AcsI RAATTY 1 cut(s) 1109
AcuI CTGAAG 3 cut(s) 579, 1157, 1326
AcyI GRCGYC 1 cut(s) 1182
AfaI GTAC 5 cut(s) 641, 658, 915, 1205, 1375
AfiI CCNNNNNNNGG 4 cut(s) 220, 386, 1315, 1459
AflIII ACRYGT 1 cut(s) 1234
AgsI TTSAA 6 cut(s) 187, 415, 817, 1106, 1159, 1270
AjnI CCWGG 1 cut(s) 916
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AleI CACNNNNGTG 1 cut(s) 1057
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 3 cut(s) 72, 938, 1140
AlwI GGATC 2 cut(s) 509, 1514
AoxI GGCC 4 cut(s) 153, 189, 222, 1473
ApeKI GCWGC 9 cut(s) 58, 270, 476, 492, 593, 596, 782, 802, 1096
ApoI RAATTY 1 cut(s) 1109
AspA2I CCTAGG 1 cut(s) 904
AspLEI GCGC 4 cut(s) 56, 58, 213, 1510
AspS9I GGNCC 2 cut(s) 223, 830
AsuHPI GGTGA 5 cut(s) 344, 766, 1044, 1085, 1238
AvaII GGWCC 1 cut(s) 830
AvrII CCTAGG 1 cut(s) 904
BanII GRGCYC 1 cut(s) 528
BbsI GAAGAC 2 cut(s) 186, 1368
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 9 cut(s) 70, 282, 479, 488, 580, 608, 769, 814, 1108
BccI CCATC 1 cut(s) 1453
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 918
BciVI GTATCC 1 cut(s) 1017
BclI TGATCA 2 cut(s) 93, 508
BcoDI GTCTC 3 cut(s) 72, 938, 1140
BfaI CTAG 3 cut(s) 723, 905, 944
BfmI CTRYAG 3 cut(s) 358, 594, 783
BfoI RGCGCY 1 cut(s) 214
BfuI GTATCC 1 cut(s) 1017
BlnI CCTAGG 1 cut(s) 904
BlpI GCTNAGC 2 cut(s) 100, 234
Bme1390I CCNGG 1 cut(s) 918
Bme18I GGWCC 1 cut(s) 830
BmgT120I GGNCC 2 cut(s) 223, 830
BmrFI CCNGG 1 cut(s) 918
BmsI GCATC 4 cut(s) 694, 833, 883, 997
BoxI GACNNNNGTC 1 cut(s) 1251
BpiI GAAGAC 2 cut(s) 186, 1368
BpmI CTGGAG 2 cut(s) 462, 1517
Bpu1102I GCTNAGC 2 cut(s) 100, 234
Bsa29I ATCGAT 2 cut(s) 422, 841
BsaAI YACGTR 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 1182
BsaJI CCNNGG 3 cut(s) 129, 394, 904
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 1120, 1150, 1310, 1340
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 386, 1315, 1459
Bse1I ACTGG 2 cut(s) 192, 1500
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 918
BseCI ATCGAT 2 cut(s) 422, 841
BseDI CCNNGG 3 cut(s) 129, 394, 904
BseGI GGATG 2 cut(s) 659, 1299
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 4 cut(s) 220, 386, 1315, 1459
BseMII CTCAG 5 cut(s) 114, 248, 356, 999, 1145
BseNI ACTGG 2 cut(s) 192, 1500
BsePI GCGCGC 1 cut(s) 54
BseXI GCAGC 9 cut(s) 70, 282, 479, 488, 580, 608, 769, 814, 1108
BseYI CCCAGC 1 cut(s) 1503
Bsh1236I CGCG 2 cut(s) 54, 56
Bsh1285I CGRYCG 2 cut(s) 65, 142
BshFI GGCC 4 cut(s) 155, 191, 224, 1475
BshVI ATCGAT 2 cut(s) 422, 841
BsiEI CGRYCG 2 cut(s) 65, 142
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 1 cut(s) 1331
BslFI GGGAC 2 cut(s) 61, 1514
BslI CCNNNNNNNGG 4 cut(s) 220, 386, 1315, 1459
BsmAI GTCTC 3 cut(s) 72, 938, 1140
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 61, 1514
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 4 cut(s) 155, 191, 224, 1475
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 5 cut(s) 93, 139, 501, 508, 1519
Bsp1720I GCTNAGC 2 cut(s) 100, 234
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 7 cut(s) 42, 147, 355, 599, 860, 1055, 1304
BspANI GGCC 4 cut(s) 155, 191, 224, 1475
BspCNI CTCAG 5 cut(s) 113, 247, 355, 998, 1144
BspDI ATCGAT 2 cut(s) 422, 841
BspFNI CGCG 2 cut(s) 54, 56
BspHI TCATGA 1 cut(s) 511
BspMAI CTGCAG 2 cut(s) 598, 787
BspPI GGATC 2 cut(s) 509, 1514
BsrBI CCGCTC 1 cut(s) 1306
BsrI ACTGG 2 cut(s) 192, 1500
BssECI CCNNGG 3 cut(s) 129, 394, 904
BssHII GCGCGC 1 cut(s) 54
BssMI GATC 5 cut(s) 93, 139, 501, 508, 1519
BssNI GRCGYC 1 cut(s) 1182
BssT1I CCWWGG 3 cut(s) 129, 394, 904
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 4 cut(s) 66, 306, 362, 811
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 1182
BstBAI YACGTR 1 cut(s) 656
BstC8I GCNNGC 4 cut(s) 56, 455, 877, 995
BstDEI CTNAG 6 cut(s) 100, 234, 342, 985, 1092, 1131
BstDSI CCRYGG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 1050
BstF5I GGATG 2 cut(s) 659, 1299
BstFNI CGCG 2 cut(s) 54, 56
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 4 cut(s) 56, 58, 213, 1510
BstKTI GATC 5 cut(s) 96, 142, 504, 511, 1522
BstMAI GTCTC 3 cut(s) 72, 938, 1140
BstMBI GATC 5 cut(s) 93, 139, 501, 508, 1519
BstMCI CGRYCG 2 cut(s) 65, 142
BstMWI GCNNNNNNNGC 7 cut(s) 230, 363, 406, 463, 599, 843, 947
BstNI CCWGG 1 cut(s) 918
BstNSI RCATGY 3 cut(s) 879, 997, 1238
BstPAI GACNNNNGTC 1 cut(s) 1251
BstPI GGTNACC 1 cut(s) 1050
BstSCI CCNGG 1 cut(s) 916
BstSFI CTRYAG 3 cut(s) 358, 594, 783
BstSNI TACGTA 1 cut(s) 656
BstUI CGCG 2 cut(s) 54, 56
BstV1I GCAGC 9 cut(s) 70, 282, 479, 488, 580, 608, 769, 814, 1108
BstV2I GAAGAC 2 cut(s) 186, 1368
BstXI CCANNNNNNTGG 1 cut(s) 670
Bsu15I ATCGAT 2 cut(s) 422, 841
BsuI GTATCC 1 cut(s) 1017
BsuRI GGCC 4 cut(s) 155, 191, 224, 1475
BsuTUI ATCGAT 2 cut(s) 422, 841
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 2 cut(s) 659, 1299
BtsI GCAGTG 2 cut(s) 792, 1064
BtsIMutI CAGTG 3 cut(s) 792, 1064, 1139
Cac8I GCNNGC 4 cut(s) 56, 455, 877, 995
CciI TCATGA 1 cut(s) 511
CfoI GCGC 4 cut(s) 56, 58, 213, 1510
Cfr13I GGNCC 2 cut(s) 223, 830
ClaI ATCGAT 2 cut(s) 422, 841
CseI GACGC 1 cut(s) 1190
CsiI ACCWGGT 1 cut(s) 916
Csp6I GTAC 5 cut(s) 640, 657, 914, 1204, 1374
CviAII CATG 9 cut(s) 130, 512, 554, 733, 834, 876, 994, 1235, 1511
CviQI GTAC 5 cut(s) 640, 657, 914, 1204, 1374
DdeI CTNAG 6 cut(s) 100, 234, 342, 985, 1092, 1131
DpnI GATC 5 cut(s) 95, 141, 503, 510, 1521
DpnII GATC 5 cut(s) 93, 139, 501, 508, 1519
EaeI YGGCCR 1 cut(s) 1473
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
EciI GGCGGA 1 cut(s) 849
Ecl136II GAGCTC 1 cut(s) 526
Eco105I TACGTA 1 cut(s) 656
Eco130I CCWWGG 3 cut(s) 129, 394, 904
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 830
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 3 cut(s) 579, 1157, 1326
Eco91I GGTNACC 1 cut(s) 1050
EcoICRI GAGCTC 1 cut(s) 526
EcoO65I GGTNACC 1 cut(s) 1050
EcoRII CCWGG 1 cut(s) 916
EcoT14I CCWWGG 3 cut(s) 129, 394, 904
EcoT22I ATGCAT 1 cut(s) 1012
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 3 cut(s) 129, 394, 904
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 9 cut(s) 133, 515, 557, 736, 837, 879, 997, 1238, 1514
FaqI GGGAC 2 cut(s) 61, 1514
FatI CATG 9 cut(s) 129, 511, 553, 732, 833, 875, 993, 1234, 1510
FauI CCCGC 2 cut(s) 49, 1311
FbaI TGATCA 2 cut(s) 93, 508
FokI GGATG 2 cut(s) 646, 1286
FriOI GRGCYC 1 cut(s) 528
FspBI CTAG 3 cut(s) 723, 905, 944
GlaI GCGC 4 cut(s) 55, 57, 212, 1509
GsaI CCCAGC 1 cut(s) 1507
GsuI CTGGAG 2 cut(s) 462, 1517
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 4 cut(s) 155, 191, 224, 1475
HapII CCGG 1 cut(s) 1331
HgaI GACGC 1 cut(s) 1190
HhaI GCGC 4 cut(s) 56, 58, 213, 1510
Hin1I GRCGYC 1 cut(s) 1182
Hin1II CATG 9 cut(s) 133, 515, 557, 736, 837, 879, 997, 1238, 1514
Hin6I GCGC 4 cut(s) 54, 56, 211, 1508
HinP1I GCGC 4 cut(s) 54, 56, 211, 1508
HpaII CCGG 1 cut(s) 1331
HphI GGTGA 5 cut(s) 344, 766, 1044, 1085, 1238
Hpy188I TCNGA 9 cut(s) 139, 242, 258, 345, 389, 437, 892, 988, 1490
Hpy188III TCNNGA 7 cut(s) 329, 412, 512, 1017, 1106, 1256, 1270
Hpy99I CGWCG 1 cut(s) 1250
HpyAV CCTTC 6 cut(s) 181, 390, 554, 791, 1041, 1410
HpyCH4III ACNGT 4 cut(s) 66, 306, 362, 811
HpyCH4IV ACGT 4 cut(s) 655, 1248, 1312, 1527
HpyCH4V TGCA 9 cut(s) 476, 538, 596, 707, 785, 875, 962, 1010, 1082
HpyF10VI GCNNNNNNNGC 7 cut(s) 230, 363, 406, 463, 599, 843, 947
HpyF3I CTNAG 6 cut(s) 100, 234, 342, 985, 1092, 1131
HpySE526I ACGT 4 cut(s) 655, 1248, 1312, 1527
Hsp92I GRCGYC 1 cut(s) 1182
Hsp92II CATG 9 cut(s) 133, 515, 557, 736, 837, 879, 997, 1238, 1514
HspAI GCGC 4 cut(s) 54, 56, 211, 1508
Ksp22I TGATCA 2 cut(s) 93, 508
Kzo9I GATC 5 cut(s) 93, 139, 501, 508, 1519
LmnI GCTCC 5 cut(s) 92, 243, 437, 724, 1311
Lsp1109I GCAGC 9 cut(s) 70, 282, 479, 488, 580, 608, 769, 814, 1108
LweI GCATC 4 cut(s) 694, 833, 883, 997
MabI ACCWGGT 1 cut(s) 916
MaeI CTAG 3 cut(s) 723, 905, 944
MaeII ACGT 4 cut(s) 655, 1248, 1312, 1527
MaeIII GTNAC 5 cut(s) 326, 728, 1050, 1149, 1421
MalI GATC 5 cut(s) 95, 141, 503, 510, 1521
MbiI CCGCTC 1 cut(s) 1306
MboI GATC 5 cut(s) 93, 139, 501, 508, 1519
MboII GAAGA 4 cut(s) 62, 158, 191, 1373
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 5 cut(s) 675, 817, 1109, 1218, 1288
MlyI GAGTC 4 cut(s) 43, 119, 399, 1138
MmeI TCCRAC 2 cut(s) 415, 878
Mph1103I ATGCAT 1 cut(s) 1012
MseI TTAA 8 cut(s) 18, 615, 954, 1035, 1163, 1241, 1295, 1349
MslI CAYNNNNRTG 1 cut(s) 1057
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 1 cut(s) 1331
MspR9I CCNGG 1 cut(s) 918
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 1 cut(s) 918
MvnI CGCG 2 cut(s) 54, 56
MwoI GCNNNNNNNGC 7 cut(s) 230, 363, 406, 463, 599, 843, 947
NcoI CCATGG 1 cut(s) 129
NdeII GATC 5 cut(s) 93, 139, 501, 508, 1519
NlaIII CATG 9 cut(s) 133, 515, 557, 736, 837, 879, 997, 1238, 1514
NmuCI GTSAC 2 cut(s) 326, 1050
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 3 cut(s) 879, 997, 1238
OliI CACNNNNGTG 1 cut(s) 1057
PaeI GCATGC 2 cut(s) 879, 997
PagI TCATGA 1 cut(s) 511
PauI GCGCGC 1 cut(s) 54
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 2 cut(s) 58, 1524
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 7 cut(s) 134, 163, 314, 349, 1039, 1379, 1427
PflMI CCANNNNNTGG 1 cut(s) 1459
Ple19I CGATCG 1 cut(s) 142
PleI GAGTC 4 cut(s) 43, 119, 398, 1138
PpsI GAGTC 4 cut(s) 43, 119, 398, 1138
Ppu21I YACGTR 1 cut(s) 656
PscI ACATGT 1 cut(s) 1234
PshAI GACNNNNGTC 1 cut(s) 1251
PsiI TTATAA 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 528
Psp6I CCWGG 1 cut(s) 916
PspEI GGTNACC 1 cut(s) 1050
PspFI CCCAGC 1 cut(s) 1503
PspGI CCWGG 1 cut(s) 916
PspPI GGNCC 2 cut(s) 223, 830
PstI CTGCAG 2 cut(s) 598, 787
PteI GCGCGC 1 cut(s) 54
PvuI CGATCG 1 cut(s) 142
RsaI GTAC 5 cut(s) 641, 658, 915, 1205, 1375
RsaNI GTAC 5 cut(s) 640, 657, 914, 1204, 1374
RseI CAYNNNNRTG 1 cut(s) 1057
SacI GAGCTC 1 cut(s) 528
SaqAI TTAA 8 cut(s) 18, 615, 954, 1035, 1163, 1241, 1295, 1349
Sau3AI GATC 5 cut(s) 93, 139, 501, 508, 1519
Sau96I GGNCC 2 cut(s) 223, 830
SchI GAGTC 4 cut(s) 43, 119, 399, 1138
ScrFI CCNGG 1 cut(s) 918
SduI GDGCHC 1 cut(s) 528
SexAI ACCWGGT 1 cut(s) 916
SfaNI GCATC 4 cut(s) 694, 833, 883, 997
SfcI CTRYAG 3 cut(s) 358, 594, 783
SinI GGWCC 1 cut(s) 830
SmiMI CAYNNNNRTG 1 cut(s) 1057
SnaBI TACGTA 1 cut(s) 656
SphI GCATGC 2 cut(s) 879, 997
Sse9I AATT 5 cut(s) 675, 817, 1109, 1218, 1288
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 7 cut(s) 42, 147, 355, 599, 860, 1055, 1304
SspMI CTAG 3 cut(s) 723, 905, 944
SstI GAGCTC 1 cut(s) 528
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 916
StyI CCWWGG 3 cut(s) 129, 394, 904
TaaI ACNGT 4 cut(s) 66, 306, 362, 811
TaiI ACGT 4 cut(s) 658, 1251, 1315, 1530
TaqI TCGA 9 cut(s) 32, 177, 228, 422, 611, 841, 1042, 1245, 1518
TasI AATT 5 cut(s) 675, 817, 1109, 1218, 1288
TatI WGTACW 1 cut(s) 1203
TauI GCSGC 2 cut(s) 150, 602
TfiI GAWTC 7 cut(s) 134, 163, 314, 349, 1039, 1379, 1427
Tru1I TTAA 8 cut(s) 18, 615, 954, 1035, 1163, 1241, 1295, 1349
Tru9I TTAA 8 cut(s) 18, 615, 954, 1035, 1163, 1241, 1295, 1349
TscAI CASTG 3 cut(s) 792, 1064, 1146
TseFI GTSAC 2 cut(s) 326, 1050
TseI GCWGC 9 cut(s) 58, 270, 476, 492, 593, 596, 782, 802, 1096
Tsp45I GTSAC 2 cut(s) 326, 1050
TspDTI ATGAA 1 cut(s) 1134
TspGWI ACGGA 4 cut(s) 298, 725, 1036, 1137
TspRI CASTG 3 cut(s) 792, 1064, 1146
Van91I CCANNNNNTGG 1 cut(s) 1459
VpaK11BI GGWCC 1 cut(s) 830
XapI RAATTY 1 cut(s) 1109
XceI RCATGY 3 cut(s) 879, 997, 1238
XmaJI CCTAGG 1 cut(s) 904
XspI CTAG 3 cut(s) 723, 905, 944
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.