MD11G1211400.v1.1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012535
Physical Location & Seq
Reverse (-)
30915645 .. 30920453
4809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1211400.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGATCACGACGGCGGCGGCGAGGCCTCTGCGATTCTTCGCCAGCTCGAAGACTTGAAGGCCAGTAAGAGCGAAATAGAGCGTCAAATCTCAGCCCTCGAAGCTCGGCTCCAAGAGATTACCCTTCCGCAGCCCAACGGCAGCGTTTCCAATGGCTCTTGTCCTCCCACTATCCCGTCCGTTGGTTCGGGCTACGGCCACGATTTGTCGCCTCAGATGATTTACCGCTACAGTCGCCACCTCTTGCTCCCTTCTTTCGGAGTCCAAGGGCAGTCAAAGCTCTTAAAGTCATCGATTTTGGTGGTCGGAGCCGGAGGATTAGGCTCGCCTGTTCTGCTGTATCTTGCAGCCTCTGGTGTTGGCCGATTGGGGGTGGTTGATCATGATGTGGTTGAGCTCAATAATATGCACAGACAGATTATCCACACTGAAAGTTTTATTGGTCAGCCAAAAGTGAAATCTGCTGCAGCTGCTTGTCATTCGATTAACTCCACTATTCAGATTGTGGAGTACCAAGAACCACTACGTACATCCAATGCTTTGGAAATTATGAGCAAATTTGATATAATAGTAGATGCAACGGACAATGCTCCTAGTCGTTACATGATTAACGATTGTTGTGTGGTGTTAGGGAAGCCTCTTGTATCTGGTGCTGCAGTGGGATTAGAAGGGCAGCTCACGGTTTTCAATTATAATGGTGGTCCATGCTATCGATGCCTATTTCCAACTCCGCCACCTACAACCGCATGTCAAAGATGTTCTGACGCTGGTGTCCTAGGAGTTGTTCCAGGTGTCATTGGCTGTCTCCAAGCCCTAGAAGCTATTAAGATTGCAAGTGCAGTTGGAGAACCACTCTCAGAACGCATGCTTTTATTTGATGCATTGTCAGGACGGATACGTAATGTTAAGATTCGAGGAAGGTCACCGCAGTGCGTAGTGTGCGGTGTGAAAGCACCATTCTCTAAGCAGCAATTTCAAGAATTTGATTATGAAAACTTCACTCATTCTCCACTGACTCCGTTACCTTTGAAGTTGAACTTACTTCAGCCAGACTCCAGAATAAACAGTAAAGAGTACAAGGAGAAACTAGTTAGTGGCGAGGCTCATGTTTTGGTCGATGTTCGTCCAGAACATCACTTCAAGATTGTTTCTCTGCCGAATTCCTTAAACATCCCACTCCCACGATTGGAGGCTCGGTTGCCGGAAATAACTTCAGCCTTAAAGGAAAAGGAAGACCAGAGGGGTACTGATTCTGGTTCAGGTGGGCATGTATATGTAATATGTAGAAGGGGTAACGATTCTCAAAGGGCTGTTCAGTATCTCCAGAAGATGGGTTTCACATCGGCCAAAGACATCATCGGAGGACTGGAGGGCTGGGCGCATGAGGTCGATCCAAACGTCCCTACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008265 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Rhodanese ThiF

Protein Analysis

471

Amino Acids

50.83

Weight (kDa)

6.67

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 77 - 312 1.4e-84 ThiF family
Rhodanese PF00581 357 - 461 1.4e-13 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55130 AT5G55130 AT5G55130
fragaria_vesca FvH4_3g23360 FvH4_3g23360
malus_domestica MD03G1195100.v1.1 MD11G1211400.v1.1
prunus_persica Prupe.4G221100_v2.0.a1
pyrus_communis pycom03g14720 pycom11g18670
rosa_chinensis RchiOBHm_Chr5g0041141
rosa_laevigata RLG00000034029
rosa_multiflora Rmu_sc0001760.1_g000005 Rmu_sc0009425.1_g000001
rosa_roxburghii Rroxscaffold_1G00039530
rosa_rugosa Rorug05G0190100
rosa_samantha Rh5BG280700 Rh5DG289000
rosa_wichuraiana Rw5G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AasI GACNNNNNNGTC 1 cut(s) 770
AccB7I CCANNNNNTGG 1 cut(s) 1330
AciI CCGC 8 cut(s) 15, 18, 128, 226, 731, 744, 926, 942
AclWI GGATC 2 cut(s) 12, 1385
AcoI YGGCCR 3 cut(s) 196, 361, 1344
AcsI RAATTY 3 cut(s) 557, 980, 1159
AcuI CTGAAG 2 cut(s) 1028, 1197
AfaI GTAC 4 cut(s) 512, 529, 1076, 1246
AfiI CCNNNNNNNGG 5 cut(s) 182, 257, 370, 1186, 1330
AgsI TTSAA 6 cut(s) 58, 688, 977, 1030, 1036, 1141
AhlI ACTAGT 1 cut(s) 1087
AjnI CCWGG 1 cut(s) 787
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AleI CACNNNNGTG 1 cut(s) 928
AluBI AGCT 7 cut(s) 46, 104, 280, 397, 470, 676, 821
AluI AGCT 7 cut(s) 46, 104, 280, 397, 470, 676, 821
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 809
AlwI GGATC 2 cut(s) 12, 1385
AlwNI CAGNNNCTG 1 cut(s) 353
AoxI GGCC 5 cut(s) 24, 60, 196, 361, 1344
ApeKI GCWGC 9 cut(s) 130, 141, 347, 464, 467, 470, 653, 673, 967
ApoI RAATTY 3 cut(s) 557, 980, 1159
AspA2I CCTAGG 1 cut(s) 775
AspLEI GCGC 1 cut(s) 1381
AspS9I GGNCC 1 cut(s) 701
AsuHPI GGTGA 1 cut(s) 915
AvaII GGWCC 1 cut(s) 701
AvrII CCTAGG 1 cut(s) 775
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 2 cut(s) 57, 1239
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 9 cut(s) 142, 153, 359, 451, 457, 479, 640, 685, 979
BccI CCATC 1 cut(s) 1324
BceAI ACGGC 3 cut(s) 28, 154, 211
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BciT130I CCWGG 1 cut(s) 789
BciVI GTATCC 1 cut(s) 888
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 809
BcuI ACTAGT 1 cut(s) 1087
BfaI CTAG 4 cut(s) 594, 776, 815, 1088
BfmI CTRYAG 3 cut(s) 229, 465, 654
BfuI GTATCC 1 cut(s) 888
BlnI CCTAGG 1 cut(s) 775
Bme1390I CCNGG 1 cut(s) 789
Bme18I GGWCC 1 cut(s) 701
BmgT120I GGNCC 1 cut(s) 701
BmiI GGNNCC 2 cut(s) 110, 310
BmrFI CCNGG 1 cut(s) 789
BmsI GCATC 3 cut(s) 565, 704, 868
BpiI GAAGAC 2 cut(s) 57, 1239
BplI GAGNNNNNCTC 2 cut(s) 837, 869
BpmI CTGGAG 3 cut(s) 1039, 1307, 1388
Bsa29I ATCGAT 2 cut(s) 293, 712
BsaAI YACGTR 2 cut(s) 527, 899
BsaJI CCNNGG 2 cut(s) 265, 775
BsaXI ACNNNNNCTCC 4 cut(s) 991, 1021, 1181, 1211
Bsc4I CCNNNNNNNGG 5 cut(s) 182, 257, 370, 1186, 1330
Bse1I ACTGG 2 cut(s) 63, 1371
BseBI CCWGG 1 cut(s) 789
BseCI ATCGAT 2 cut(s) 293, 712
BseDI CCNNGG 2 cut(s) 265, 775
BseGI GGATG 2 cut(s) 530, 1170
BseLI CCNNNNNNNGG 5 cut(s) 182, 257, 370, 1186, 1330
BseMII CTCAG 3 cut(s) 105, 227, 870
BseNI ACTGG 2 cut(s) 63, 1371
BseXI GCAGC 9 cut(s) 142, 153, 359, 451, 457, 479, 640, 685, 979
BseYI CCCAGC 1 cut(s) 1374
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 5 cut(s) 26, 62, 198, 363, 1346
BshVI ATCGAT 2 cut(s) 293, 712
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 2 cut(s) 312, 1202
BslFI GGGAC 1 cut(s) 1385
BslI CCNNNNNNNGG 5 cut(s) 182, 257, 370, 1186, 1330
BsmAI GTCTC 1 cut(s) 809
BsmFI GGGAC 1 cut(s) 1385
BsnI GGCC 5 cut(s) 26, 62, 198, 363, 1346
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 3 cut(s) 4, 379, 1390
BspACI CCGC 8 cut(s) 15, 18, 128, 226, 731, 744, 926, 942
BspANI GGCC 5 cut(s) 26, 62, 198, 363, 1346
BspCNI CTCAG 3 cut(s) 104, 226, 869
BspDI ATCGAT 2 cut(s) 293, 712
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 110, 310
BspMAI CTGCAG 2 cut(s) 469, 658
BspPI GGATC 2 cut(s) 12, 1385
BsrI ACTGG 2 cut(s) 63, 1371
BssECI CCNNGG 2 cut(s) 265, 775
BssMI GATC 3 cut(s) 4, 379, 1390
BssT1I CCWWGG 2 cut(s) 265, 775
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 3 cut(s) 233, 682, 1067
BstBAI YACGTR 2 cut(s) 527, 899
BstC8I GCNNGC 3 cut(s) 44, 326, 866
BstDEI CTNAG 4 cut(s) 91, 213, 856, 963
BstEII GGTNACC 1 cut(s) 921
BstF5I GGATG 2 cut(s) 530, 1170
BstHHI GCGC 1 cut(s) 1381
BstKTI GATC 3 cut(s) 7, 382, 1393
BstMAI GTCTC 1 cut(s) 809
BstMBI GATC 3 cut(s) 4, 379, 1390
BstMWI GCNNNNNNNGC 8 cut(s) 101, 234, 277, 334, 470, 714, 818, 939
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 3 cut(s) 750, 868, 1271
BstPI GGTNACC 1 cut(s) 921
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 3 cut(s) 229, 465, 654
BstSNI TACGTA 2 cut(s) 527, 899
BstV1I GCAGC 9 cut(s) 142, 153, 359, 451, 457, 479, 640, 685, 979
BstV2I GAAGAC 2 cut(s) 57, 1239
BstXI CCANNNNNNTGG 1 cut(s) 541
Bsu15I ATCGAT 2 cut(s) 293, 712
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 5 cut(s) 26, 62, 198, 363, 1346
BsuTUI ATCGAT 2 cut(s) 293, 712
BtsCI GGATG 2 cut(s) 530, 1170
BtsI GCAGTG 2 cut(s) 663, 935
BtsIMutI CAGTG 4 cut(s) 426, 663, 935, 1010
Cac8I GCNNGC 3 cut(s) 44, 326, 866
CaiI CAGNNNCTG 1 cut(s) 353
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 1381
Cfr13I GGNCC 1 cut(s) 701
ClaI ATCGAT 2 cut(s) 293, 712
CseI GACGC 2 cut(s) 71, 773
Csp6I GTAC 4 cut(s) 511, 528, 1075, 1245
CviAII CATG 8 cut(s) 383, 604, 705, 747, 865, 1106, 1268, 1382
CviQI GTAC 4 cut(s) 511, 528, 1075, 1245
DdeI CTNAG 4 cut(s) 91, 213, 856, 963
DpnI GATC 3 cut(s) 6, 381, 1392
DpnII GATC 3 cut(s) 4, 379, 1390
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 3 cut(s) 196, 361, 1344
EciI GGCGGA 1 cut(s) 720
Ecl136II GAGCTC 1 cut(s) 397
Eco105I TACGTA 2 cut(s) 527, 899
Eco130I CCWWGG 2 cut(s) 265, 775
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 701
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 1028, 1197
Eco91I GGTNACC 1 cut(s) 921
EcoICRI GAGCTC 1 cut(s) 397
EcoO65I GGTNACC 1 cut(s) 921
EcoRI GAATTC 1 cut(s) 1159
EcoRII CCWGG 1 cut(s) 787
EcoT14I CCWWGG 2 cut(s) 265, 775
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 265, 775
FaeI CATG 8 cut(s) 386, 607, 708, 750, 868, 1109, 1271, 1385
FalI AAGNNNNNCTT 2 cut(s) 1022, 1054
FaqI GGGAC 1 cut(s) 1385
FatI CATG 8 cut(s) 382, 603, 704, 746, 864, 1105, 1267, 1381
FbaI TGATCA 1 cut(s) 379
FokI GGATG 2 cut(s) 517, 1157
FriOI GRGCYC 1 cut(s) 399
FspBI CTAG 4 cut(s) 594, 776, 815, 1088
GlaI GCGC 1 cut(s) 1380
GsaI CCCAGC 1 cut(s) 1378
GsuI CTGGAG 3 cut(s) 1039, 1307, 1388
HaeIII GGCC 5 cut(s) 26, 62, 198, 363, 1346
HapII CCGG 2 cut(s) 312, 1202
HgaI GACGC 2 cut(s) 71, 773
HhaI GCGC 1 cut(s) 1381
Hin1II CATG 8 cut(s) 386, 607, 708, 750, 868, 1109, 1271, 1385
Hin6I GCGC 1 cut(s) 1379
HinP1I GCGC 1 cut(s) 1379
HinfI GANTC 7 cut(s) 34, 261, 910, 1015, 1052, 1250, 1298
HpaII CCGG 2 cut(s) 312, 1202
HphI GGTGA 1 cut(s) 915
Hpy188I TCNGA 7 cut(s) 216, 260, 308, 501, 763, 859, 1361
Hpy188III TCNNGA 8 cut(s) 8, 383, 888, 977, 1056, 1127, 1141, 1324
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 6 cut(s) 52, 134, 261, 662, 912, 1281
HpyCH4III ACNGT 3 cut(s) 233, 682, 1067
HpyCH4IV ACGT 3 cut(s) 526, 898, 1398
HpyCH4V TGCA 8 cut(s) 347, 409, 467, 578, 656, 833, 839, 881
HpyF10VI GCNNNNNNNGC 8 cut(s) 101, 234, 277, 334, 470, 714, 818, 939
HpyF3I CTNAG 4 cut(s) 91, 213, 856, 963
HpySE526I ACGT 3 cut(s) 526, 898, 1398
Hsp92II CATG 8 cut(s) 386, 607, 708, 750, 868, 1109, 1271, 1385
HspAI GCGC 1 cut(s) 1379
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 3 cut(s) 4, 379, 1390
LmnI GCTCC 4 cut(s) 114, 252, 308, 595
Lsp1109I GCAGC 9 cut(s) 142, 153, 359, 451, 457, 479, 640, 685, 979
LweI GCATC 3 cut(s) 565, 704, 868
MaeI CTAG 4 cut(s) 594, 776, 815, 1088
MaeII ACGT 3 cut(s) 526, 898, 1398
MaeIII GTNAC 4 cut(s) 599, 921, 1020, 1292
MalI GATC 3 cut(s) 6, 381, 1392
MboI GATC 3 cut(s) 4, 379, 1390
MboII GAAGA 4 cut(s) 29, 62, 1244, 1339
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 6 cut(s) 546, 557, 688, 971, 980, 1159
MlyI GAGTC 3 cut(s) 270, 1009, 1046
MmeI TCCRAC 3 cut(s) 286, 749, 823
Mph1103I ATGCAT 1 cut(s) 883
MseI TTAA 7 cut(s) 284, 486, 609, 825, 906, 1166, 1220
MslI CAYNNNNRTG 2 cut(s) 928, 1272
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 312, 1202
MspR9I CCNGG 1 cut(s) 789
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 8 cut(s) 101, 234, 277, 334, 470, 714, 818, 939
NdeII GATC 3 cut(s) 4, 379, 1390
NlaIII CATG 8 cut(s) 386, 607, 708, 750, 868, 1109, 1271, 1385
NlaIV GGNNCC 2 cut(s) 110, 310
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 921
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 3 cut(s) 750, 868, 1271
OliI CACNNNNGTG 1 cut(s) 928
PaeI GCATGC 1 cut(s) 868
PagI TCATGA 1 cut(s) 382
PceI AGGCCT 1 cut(s) 26
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 1395
PfeI GAWTC 4 cut(s) 34, 910, 1250, 1298
PflMI CCANNNNNTGG 1 cut(s) 1330
PleI GAGTC 3 cut(s) 269, 1009, 1046
PpsI GAGTC 3 cut(s) 269, 1009, 1046
Ppu21I YACGTR 2 cut(s) 527, 899
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 787
PspEI GGTNACC 1 cut(s) 921
PspFI CCCAGC 1 cut(s) 1374
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 2 cut(s) 110, 310
PspPI GGNCC 1 cut(s) 701
PstI CTGCAG 2 cut(s) 469, 658
PstNI CAGNNNCTG 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 4 cut(s) 512, 529, 1076, 1246
RsaNI GTAC 4 cut(s) 511, 528, 1075, 1245
RseI CAYNNNNRTG 2 cut(s) 928, 1272
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 7 cut(s) 284, 486, 609, 825, 906, 1166, 1220
Sau3AI GATC 3 cut(s) 4, 379, 1390
Sau96I GGNCC 1 cut(s) 701
SchI GAGTC 3 cut(s) 270, 1009, 1046
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 3 cut(s) 565, 704, 868
SfcI CTRYAG 3 cut(s) 229, 465, 654
SinI GGWCC 1 cut(s) 701
SmiMI CAYNNNNRTG 2 cut(s) 928, 1272
SnaBI TACGTA 2 cut(s) 527, 899
SpeI ACTAGT 1 cut(s) 1087
SphI GCATGC 1 cut(s) 868
Sse9I AATT 6 cut(s) 546, 557, 688, 971, 980, 1159
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 8 cut(s) 15, 18, 128, 226, 731, 744, 926, 942
SspMI CTAG 4 cut(s) 594, 776, 815, 1088
SstI GAGCTC 1 cut(s) 399
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 787
StyI CCWWGG 2 cut(s) 265, 775
TaaI ACNGT 3 cut(s) 233, 682, 1067
TaiI ACGT 3 cut(s) 529, 901, 1401
TaqI TCGA 8 cut(s) 48, 99, 293, 482, 712, 913, 1116, 1389
TasI AATT 6 cut(s) 546, 557, 688, 971, 980, 1159
TatI WGTACW 1 cut(s) 1074
TauI GCSGC 2 cut(s) 18, 21
TfiI GAWTC 4 cut(s) 34, 910, 1250, 1298
Tru1I TTAA 7 cut(s) 284, 486, 609, 825, 906, 1166, 1220
Tru9I TTAA 7 cut(s) 284, 486, 609, 825, 906, 1166, 1220
TscAI CASTG 4 cut(s) 433, 663, 935, 1017
TseFI GTSAC 1 cut(s) 921
TseI GCWGC 9 cut(s) 130, 141, 347, 464, 467, 470, 653, 673, 967
Tsp45I GTSAC 1 cut(s) 921
TspDTI ATGAA 1 cut(s) 1005
TspGWI ACGGA 4 cut(s) 169, 596, 907, 1008
TspRI CASTG 4 cut(s) 433, 663, 935, 1017
Van91I CCANNNNNTGG 1 cut(s) 1330
VpaK11BI GGWCC 1 cut(s) 701
XapI RAATTY 3 cut(s) 557, 980, 1159
XceI RCATGY 3 cut(s) 750, 868, 1271
XmaJI CCTAGG 1 cut(s) 775
XspI CTAG 4 cut(s) 594, 776, 815, 1088
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.