AT5G55530

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006102
Physical Location & Seq
Forward (+)
22492892 .. 22496064
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55530.1

Sequence Viewer

Length: 1218 bp
ATGGACTCCCCACAATCTGTTGTTTCACCATTCAAGATTGGTGAGTCAGAGAATGAGAACTCAAATTCTGTGCAGAGTTCTGGAAACCAATCGAATGGCATAAATTCCAATGGGAAAGATTCTAAAAGTTGTGGCCGGCAAGACCTTGTTGGTGCTCTTGAGGTTTATGTCCATCAGGCAAGGGATATCCATAACATTTGTATATATCACAAGCAAGACGTTTATGCCAAGCTTTGCCTTACAAGTGATCCTGATAAGTCTGTCTCGACCAAAATCATCAATGGCGGTGGGAGGAATCCGGTTTTTGATGACAATGTTAAGCTTGATGTCAGGGTTCTGGACACTTCCCTCAAATGTGAGATTTACATGATGAGCAGGGTGAAGAATTATCTTGAGGACCAGCTGCTTGGTTTTACTCTGGTTCCTATGTCGGAACTGCTCTTCAAGAATGGGAAACTCGAGAAAGAGTTCTCTCTTTCTTCCACTGATCTCTACCATTCGCCAGCTGGTTTTGTTCAATTGTCACTCTCCTATTATGGATCCTACCCTGACGTGATGGCTATTCCCTCTATGCCTTCGTCTGTTTCTATTGATGAAACTACCAAGGATCCAGAAGGGTCTGAATCAGTTCCGGGTGAGTTGGATAAGATAGAGTTTCCAGATCCTAATGTTGCTAATGAAAATGAGAAGATGGTTTCCGAGTATTTTGGGATCTCTTGTTCCACTATTGACTCAGAAACTTCTGACAGCTTGGTTACTTCCGATGCTGAGAATCATGTGACTAATTCTGTCACTTCTATACTGAAGCAAGATTCACCTGAGAGCAGCAATGCAACAAATGGAGCTGCTTCTCCTCACGCTTCAGCTCACTCCGCCACAGAAACACCAAACCATGAACATCTCTCTGTCGTGAACTCCAAAGCGAGTTCCCAGGAGTCAGAGAGCGAGGCTTCTGGTGAGACATCTGAGGAGAAGACTGTGAAATCCGTCCTCACTGTGAAGGTTGAGCCAGAGTCAAAGGTGGTGCAGCAGGATATAGTCGACATGTACATGAAAAGCATGCAACAGTTTACAGATTCACTGGCTAAGATGAAGCTTCCTCTGGACATTGACAGCCCAACAAAATCAGAAAATTCAAGCTCTGATTCTCAGAAGCTGCCAACACCAAAGAGCAACAACGGATCCCGTGTGTTCTATGGGAGCAGGCCTTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

405

Amino Acids

44.13

Weight (kDa)

4.73

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 51 - 155 3.8e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1041
AciI CCGC 2 cut(s) 285, 873
AclWI GGATC 9 cut(s) 242, 534, 547, 602, 615, 656, 719, 1176, 1189
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 3 cut(s) 64, 103, 1132
AcuI CTGAAG 2 cut(s) 824, 846
AfaI GTAC 1 cut(s) 1049
AflIII ACRYGT 1 cut(s) 1044
AgsI TTSAA 4 cut(s) 34, 445, 518, 1137
AjiI CACGTC 1 cut(s) 553
AjnI CCWGG 1 cut(s) 930
AloI GAACNNNNNNTCC 2 cut(s) 703, 735
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 268, 953
AlwI GGATC 9 cut(s) 242, 534, 547, 602, 615, 656, 719, 1176, 1189
AlwNI CAGNNNCTG 1 cut(s) 1156
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 2 cut(s) 133, 1205
ApeKI GCWGC 5 cut(s) 403, 825, 845, 1027, 1156
ApoI RAATTY 3 cut(s) 64, 103, 1132
Asp700I GAANNNNTTC 2 cut(s) 467, 627
AspS9I GGNCC 1 cut(s) 397
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 6 cut(s) 18, 53, 391, 647, 807, 968
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 1 cut(s) 397
BamHI GGATCC 3 cut(s) 539, 607, 1181
BbsI GAAGAC 1 cut(s) 980
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 5 cut(s) 390, 832, 837, 1039, 1143
BccI CCATC 3 cut(s) 180, 550, 685
BciT130I CCWGG 1 cut(s) 932
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 2 cut(s) 268, 953
BisI GCNGC 5 cut(s) 404, 826, 846, 1028, 1157
BlsI GCNGC 5 cut(s) 405, 827, 847, 1029, 1158
Bme1390I CCNGG 2 cut(s) 633, 932
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 458
BmgBI CACGTC 1 cut(s) 553
BmgT120I GGNCC 1 cut(s) 397
BmiI GGNNCC 4 cut(s) 423, 541, 609, 1183
BmrFI CCNGG 2 cut(s) 633, 932
BmsI GCATC 1 cut(s) 754
BpiI GAAGAC 1 cut(s) 980
BpuEI CTTGAG 2 cut(s) 179, 413
BpuMI CCSGG 1 cut(s) 633
BsaJI CCNNGG 2 cut(s) 603, 930
BsaWI WCCGGW 1 cut(s) 298
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 1 cut(s) 1086
Bse3DI GCAATG 1 cut(s) 835
BseBI CCWGG 1 cut(s) 932
BseDI CCNNGG 2 cut(s) 603, 930
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 5 cut(s) 747, 759, 810, 957, 1163
BseNI ACTGG 1 cut(s) 1086
BseRI GAGGAG 2 cut(s) 843, 983
BseXI GCAGC 5 cut(s) 390, 832, 837, 1039, 1143
BsgI GTGCAG 2 cut(s) 92, 1046
BshFI GGCC 2 cut(s) 135, 1207
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 3 cut(s) 136, 299, 632
BsmAI GTCTC 2 cut(s) 268, 953
BsnI GGCC 2 cut(s) 135, 1207
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 157
Bsp1407I TGTACA 1 cut(s) 1047
Bsp143I GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
BspACI CCGC 2 cut(s) 285, 873
BspANI GGCC 2 cut(s) 135, 1207
BspCNI CTCAG 5 cut(s) 746, 760, 811, 958, 1162
BspLI GGNNCC 4 cut(s) 423, 541, 609, 1183
BspPI GGATC 9 cut(s) 242, 534, 547, 602, 615, 656, 719, 1176, 1189
BspQI GCTCTTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 835
BsrFI RCCGGY 1 cut(s) 135
BsrGI TGTACA 1 cut(s) 1047
BsrI ACTGG 1 cut(s) 1086
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 2 cut(s) 603, 930
BssMI GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
BssT1I CCWWGG 1 cut(s) 603
Bst2UI CCWGG 1 cut(s) 932
Bst4CI ACNGT 3 cut(s) 979, 997, 1068
Bst6I CTCTTC 1 cut(s) 446
BstAUI TGTACA 1 cut(s) 1047
BstC8I GCNNGC 4 cut(s) 137, 504, 1061, 1205
BstDEI CTNAG 6 cut(s) 733, 768, 819, 966, 1086, 1149
BstKTI GATC 7 cut(s) 250, 490, 542, 610, 664, 714, 1184
BstMAI GTCTC 2 cut(s) 268, 953
BstMBI GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
BstMWI GCNNNNNNNGC 1 cut(s) 872
BstNI CCWGG 1 cut(s) 932
BstNSI RCATGY 2 cut(s) 1048, 1063
BstSCI CCNGG 2 cut(s) 631, 930
BstV1I GCAGC 5 cut(s) 390, 832, 837, 1039, 1143
BstV2I GAAGAC 1 cut(s) 980
BstX2I RGATCY 5 cut(s) 539, 607, 661, 711, 1181
BstXI CCANNNNNNTGG 2 cut(s) 95, 407
BstYI RGATCY 5 cut(s) 539, 607, 661, 711, 1181
BsuRI GGCC 2 cut(s) 135, 1207
BtrI CACGTC 1 cut(s) 553
BtsIMutI CAGTG 3 cut(s) 483, 993, 1079
Cac8I GCNNGC 4 cut(s) 137, 504, 1061, 1205
CaiI CAGNNNCTG 1 cut(s) 1156
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 397
Csp6I GTAC 1 cut(s) 1048
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 6 cut(s) 367, 776, 893, 1045, 1051, 1060
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 6 cut(s) 733, 768, 819, 966, 1086, 1149
DpnI GATC 7 cut(s) 249, 489, 541, 609, 663, 713, 1183
DpnII GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 446
EciI GGCGGA 1 cut(s) 862
Eco130I CCWWGG 1 cut(s) 603
Eco147I AGGCCT 1 cut(s) 1207
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 824, 846
Eco88I CYCGRG 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 930
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 6 cut(s) 370, 779, 896, 1048, 1054, 1063
FatI CATG 6 cut(s) 366, 775, 892, 1044, 1050, 1059
FblI GTMKAC 1 cut(s) 1041
Fnu4HI GCNGC 5 cut(s) 404, 826, 846, 1028, 1157
Fsp4HI GCNGC 5 cut(s) 404, 826, 846, 1028, 1157
GluI GCNGC 5 cut(s) 404, 826, 846, 1028, 1157
HaeIII GGCC 2 cut(s) 135, 1207
HapII CCGG 3 cut(s) 136, 299, 632
Hin1II CATG 6 cut(s) 370, 779, 896, 1048, 1054, 1063
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HindIII AAGCTT 3 cut(s) 230, 320, 1094
HpaII CCGG 3 cut(s) 136, 299, 632
HphI GGTGA 6 cut(s) 18, 53, 391, 647, 807, 968
Hpy166II GTNNAC 3 cut(s) 913, 1042, 1071
Hpy8I GTNNAC 3 cut(s) 913, 1042, 1071
HpyAV CCTTC 3 cut(s) 585, 608, 994
HpyCH4III ACNGT 3 cut(s) 979, 997, 1068
HpyCH4IV ACGT 2 cut(s) 219, 552
HpyCH4V TGCA 4 cut(s) 73, 833, 1027, 1063
HpyF10VI GCNNNNNNNGC 1 cut(s) 872
HpyF3I CTNAG 6 cut(s) 733, 768, 819, 966, 1086, 1149
HpySE526I ACGT 2 cut(s) 219, 552
Hsp92II CATG 6 cut(s) 370, 779, 896, 1048, 1054, 1063
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Kzo9I GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 2 cut(s) 842, 1200
Lsp1109I GCAGC 5 cut(s) 390, 832, 837, 1039, 1143
LweI GCATC 1 cut(s) 754
MaeII ACGT 2 cut(s) 219, 552
MaeIII GTNAC 4 cut(s) 522, 754, 778, 790
MalI GATC 7 cut(s) 249, 489, 541, 609, 663, 713, 1183
MboI GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
MboII GAAGA 6 cut(s) 394, 433, 471, 700, 985, 1204
MfeI CAATTG 1 cut(s) 518
MflI RGATCY 5 cut(s) 539, 607, 661, 711, 1181
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 6 cut(s) 64, 103, 385, 518, 784, 1132
MlyI GAGTC 4 cut(s) 53, 725, 944, 1022
MmeI TCCRAC 2 cut(s) 411, 621
MroNI GCCGGC 1 cut(s) 135
MroXI GAANNNNTTC 2 cut(s) 467, 627
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 1049
MspA1I CMGCKG 2 cut(s) 403, 506
MspI CCGG 3 cut(s) 136, 299, 632
MspR9I CCNGG 2 cut(s) 633, 932
MunI CAATTG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 932
MwoI GCNNNNNNNGC 1 cut(s) 872
NaeI GCCGGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 633
NdeII GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 6 cut(s) 370, 779, 896, 1048, 1054, 1063
NlaIV GGNNCC 4 cut(s) 423, 541, 609, 1183
NmuCI GTSAC 3 cut(s) 522, 778, 790
NspI RCATGY 2 cut(s) 1048, 1063
PaeI GCATGC 1 cut(s) 1063
PaeR7I CTCGAG 1 cut(s) 458
PceI AGGCCT 1 cut(s) 1207
PciI ACATGT 1 cut(s) 1044
PciSI GCTCTTC 1 cut(s) 446
PdiI GCCGGC 1 cut(s) 137
PdmI GAANNNNTTC 2 cut(s) 467, 627
PfeI GAWTC 7 cut(s) 119, 295, 623, 772, 812, 1076, 1145
PkrI GCNGC 5 cut(s) 405, 827, 847, 1029, 1158
PleI GAGTC 4 cut(s) 52, 725, 943, 1021
PpsI GAGTC 4 cut(s) 52, 725, 943, 1021
PscI ACATGT 1 cut(s) 1044
Psp6I CCWGG 1 cut(s) 930
PspGI CCWGG 1 cut(s) 930
PspN4I GGNNCC 4 cut(s) 423, 541, 609, 1183
PspPI GGNCC 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 1156
PsuI RGATCY 5 cut(s) 539, 607, 661, 711, 1181
PvuII CAGCTG 2 cut(s) 403, 506
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 1 cut(s) 1049
SalI GTCGAC 1 cut(s) 1040
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 5 cut(s) 404, 826, 846, 1028, 1157
Sau3AI GATC 7 cut(s) 247, 487, 539, 607, 661, 711, 1181
Sau96I GGNCC 1 cut(s) 397
SchI GAGTC 4 cut(s) 53, 725, 944, 1022
ScrFI CCNGG 2 cut(s) 633, 932
SduI GDGCHC 1 cut(s) 157
SfaNI GCATC 1 cut(s) 754
Sfr274I CTCGAG 1 cut(s) 458
SinI GGWCC 1 cut(s) 397
SlaI CTCGAG 1 cut(s) 458
SmiMI CAYNNNNRTG 1 cut(s) 1049
SmlI CTYRAG 3 cut(s) 158, 392, 458
SmoI CTYRAG 3 cut(s) 158, 392, 458
SphI GCATGC 1 cut(s) 1063
Sse9I AATT 6 cut(s) 64, 103, 385, 518, 784, 1132
SseBI AGGCCT 1 cut(s) 1207
SsiI CCGC 2 cut(s) 285, 873
StuI AGGCCT 1 cut(s) 1207
StyD4I CCNGG 2 cut(s) 631, 930
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 3 cut(s) 979, 997, 1068
TaiI ACGT 2 cut(s) 222, 555
TaqI TCGA 4 cut(s) 92, 266, 459, 1041
TasI AATT 6 cut(s) 64, 103, 385, 518, 784, 1132
TatI WGTACW 1 cut(s) 1047
TfiI GAWTC 7 cut(s) 119, 295, 623, 772, 812, 1076, 1145
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 3 cut(s) 490, 1000, 1086
TseFI GTSAC 3 cut(s) 522, 778, 790
TseI GCWGC 5 cut(s) 403, 825, 845, 1027, 1156
Tsp45I GTSAC 3 cut(s) 522, 778, 790
TspDTI ATGAA 5 cut(s) 609, 693, 909, 1067, 1106
TspGWI ACGGA 2 cut(s) 976, 1194
TspRI CASTG 3 cut(s) 490, 1000, 1086
VpaK11BI GGWCC 1 cut(s) 397
XapI RAATTY 3 cut(s) 64, 103, 1132
XceI RCATGY 2 cut(s) 1048, 1063
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XhoI CTCGAG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 1041
XmnI GAANNNNTTC 2 cut(s) 467, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.