FvH4_3g23940

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006102
Physical Location & Seq
Forward (+)
17035122 .. 17038181
3060 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23940.t3

Sequence Viewer

Length: 1296 bp
ATGGATTCCCCTCAATCTGTTGTCTCTCCATTCAAAACCTCTGTTGTTTCCGAGCATGAGAAGCAGAAATCTGACTGTTCTGTTCGGTCCAACTCGGGACCATATTCTAATGGAATTGAGGCCAATAGACAGGAAACCAATGTTTGTGATGTAGATGACTACATTGGTGTTATTGATGTTTATGTTCATCAGGCCAGGGACATACACAACATCTGCATTTACCACAAGCAAGATGTGTATGCTAAGCTTTTCCTGACAAGTGATCCAGAACGCACCGTATCCACCAAAACCATTAATGGTGGCGGTCAGAATCCAGTCTTCGATGACAAGGTCCAGCTCAATGTTCGGACTGTTGATGCCTCCGTCAAATGTGAGATTTGGATGCTGAGCAGGGTGAAGAATTATCTTGAAGATCAGTTGCTGGGGTTTGCTTTGGTGCCTATGACAGAGGTCCTCATCAAGAATGGAAGGTTGGAGAAAGAATTCCCTCTTTCTTCAACCGATCTCTTTCATTCCCCAGCAGGGTTTGTGAAATTGTCTCTCTCTTATGCTGGAGATTCACCAGAAGTGATGTCCTTGACCTCAAATCCTATTATGGAGGACACAGAGATAACTGATTCTATTCCTTGTGAAGAATTAGATAAGATAGAGTTCCCAGACCCAGATATTGCCAATGAAGACCAGATGATGGTTTCGGAGTATATTAAGATCCCATGTTCGGAGTTTGAGTCTCAGAGCTCTGAGAGCTTTGTTACTGCTGATGGTGAAACTCATGTCAGTTCCGAAGTGGCCGTTCATGCTGTTGAAAGCGCCATTCATGCTGTTGAAAGCTTCCCGACGGCTGCTTTTGGATCTATTCAGGCTCAGAAGCTTGACTCTCCCCCAAGCAGTGTGTCAACTAATGGGGTTTCATCTCCATCAGTCCAGGCAAGCTCAGAGTCATTTGACGCTCCAGCAACTTCGGAATCGCCAAATCAGGAACAAGTTTCTGCCTCAAAAGAGAAAAGGGCAGACCTTAAAGATGGTGAGACTGATTCATCTGGTGAGGTGCCTAGTGATGCAATTGCAAAACCCGTGGTCACTGTCAACATTCCAGAGCAAAATGTGGTGCAGCAGGATTTTGTAGACATGTACATGAAAAGCATGCAGCAGTTTACTGAGTCTTTGGCAAAAATGAAGCTTCCTGTGGACATCGATTCACCAACCAGTTCAGGAAATTCGAGCTCTGATCAGAATTTAGCTACACCAAAGACAAATGGCTCCCGTGTGTTTTATGGGAGTAGAGCTTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

432

Amino Acids

47.02

Weight (kDa)

4.47

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 57 - 162 2e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 436, 1048
AccB7I CCANNNNNTGG 1 cut(s) 688
AccI GTMKAC 1 cut(s) 1125
AciI CCGC 1 cut(s) 303
AclWI GGATC 3 cut(s) 257, 703, 859
AcoI YGGCCR 1 cut(s) 789
AcsI RAATTY 3 cut(s) 482, 1216, 1234
AfaI GTAC 1 cut(s) 1133
AfiI CCNNNNNNNGG 3 cut(s) 522, 688, 718
AflIII ACRYGT 1 cut(s) 1128
AgsI TTSAA 5 cut(s) 34, 410, 498, 806, 827
AjnI CCWGG 2 cut(s) 194, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
Alw21I GWGCWC 2 cut(s) 740, 1226
Alw26I GTCTC 4 cut(s) 28, 543, 735, 1022
AlwI GGATC 3 cut(s) 257, 703, 859
AlwNI CAGNNNCTG 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 3 cut(s) 120, 192, 789
ApeKI GCWGC 3 cut(s) 842, 1111, 1147
ApoI RAATTY 3 cut(s) 482, 1216, 1234
AseI ATTAAT 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 812
AspS9I GGNCC 4 cut(s) 87, 98, 331, 451
AsuHPI GGTGA 6 cut(s) 406, 552, 776, 1037, 1055, 1191
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 4 cut(s) 87, 98, 331, 451
BanI GGYRCC 2 cut(s) 436, 1048
BanII GRGCYC 2 cut(s) 740, 1226
BbsI GAAGAC 2 cut(s) 310, 684
Bbv12I GWGCWC 2 cut(s) 740, 1226
BbvI GCAGC 3 cut(s) 829, 1123, 1159
BccI CCATC 4 cut(s) 682, 755, 925, 1016
BceAI ACGGC 2 cut(s) 776, 855
BciT130I CCWGG 2 cut(s) 196, 926
BciVI GTATCC 1 cut(s) 289
BclI TGATCA 1 cut(s) 1228
BcoDI GTCTC 4 cut(s) 28, 543, 735, 1022
BfaI CTAG 1 cut(s) 1053
BfoI RGCGCY 1 cut(s) 813
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 3 cut(s) 843, 1112, 1148
BlpI GCTNAGC 2 cut(s) 243, 386
BlsI GCNGC 3 cut(s) 844, 1113, 1149
Bme1390I CCNGG 2 cut(s) 196, 926
Bme18I GGWCC 4 cut(s) 87, 98, 331, 451
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 4 cut(s) 87, 98, 331, 451
BmiI GGNNCC 4 cut(s) 99, 438, 1050, 1261
BmrFI CCNGG 2 cut(s) 196, 926
BmsI GCATC 3 cut(s) 346, 372, 1048
BoxI GACNNNNGTC 1 cut(s) 449
BpiI GAAGAC 2 cut(s) 310, 684
BpmI CTGGAG 2 cut(s) 573, 936
Bpu1102I GCTNAGC 2 cut(s) 243, 386
Bsa29I ATCGAT 1 cut(s) 1194
BsaJI CCNNGG 2 cut(s) 195, 1074
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 3 cut(s) 522, 688, 718
Bse1I ACTGG 2 cut(s) 314, 1206
BseBI CCWGG 2 cut(s) 196, 926
BseCI ATCGAT 1 cut(s) 1194
BseDI CCNNGG 2 cut(s) 195, 1074
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 3 cut(s) 522, 688, 718
BseMII CTCAG 6 cut(s) 377, 732, 746, 878, 948, 1149
BseNI ACTGG 2 cut(s) 314, 1206
BseXI GCAGC 3 cut(s) 829, 1123, 1159
BseYI CCCAGC 2 cut(s) 421, 517
BsgI GTGCAG 1 cut(s) 1130
BshFI GGCC 3 cut(s) 122, 194, 791
BshNI GGYRCC 2 cut(s) 436, 1048
BshVI ATCGAT 1 cut(s) 1194
BsiHKAI GWGCWC 2 cut(s) 740, 1226
BsiHKCI CYCGRG 1 cut(s) 94
BslFI GGGAC 2 cut(s) 111, 212
BslI CCNNNNNNNGG 3 cut(s) 522, 688, 718
BsmAI GTCTC 4 cut(s) 28, 543, 735, 1022
BsmFI GGGAC 2 cut(s) 111, 212
BsnI GGCC 3 cut(s) 122, 194, 791
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 2 cut(s) 740, 1226
Bsp1407I TGTACA 1 cut(s) 1131
Bsp143I GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
Bsp1720I GCTNAGC 2 cut(s) 243, 386
BspACI CCGC 1 cut(s) 303
BspANI GGCC 3 cut(s) 122, 194, 791
BspCNI CTCAG 6 cut(s) 378, 733, 745, 877, 947, 1150
BspDI ATCGAT 1 cut(s) 1194
BspLI GGNNCC 4 cut(s) 99, 438, 1050, 1261
BspPI GGATC 3 cut(s) 257, 703, 859
BspT107I GGYRCC 2 cut(s) 436, 1048
BsrGI TGTACA 1 cut(s) 1131
BsrI ACTGG 2 cut(s) 314, 1206
BssECI CCNNGG 2 cut(s) 195, 1074
BssMI GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
Bst2UI CCWGG 2 cut(s) 196, 926
Bst4CI ACNGT 4 cut(s) 77, 277, 352, 1084
BstAUI TGTACA 1 cut(s) 1131
BstC8I GCNNGC 2 cut(s) 931, 1145
BstDEI CTNAG 7 cut(s) 243, 386, 732, 741, 864, 934, 1158
BstDSI CCRYGG 1 cut(s) 1074
BstF5I GGATG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 813
BstHHI GCGC 1 cut(s) 812
BstKTI GATC 6 cut(s) 265, 415, 505, 711, 854, 1231
BstMAI GTCTC 4 cut(s) 28, 543, 735, 1022
BstMBI GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
BstMWI GCNNNNNNNGC 4 cut(s) 61, 744, 797, 818
BstNI CCWGG 2 cut(s) 196, 926
BstNSI RCATGY 2 cut(s) 1132, 1147
BstPAI GACNNNNGTC 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 194, 924
BstV1I GCAGC 3 cut(s) 829, 1123, 1159
BstV2I GAAGAC 2 cut(s) 310, 684
BstX2I RGATCY 2 cut(s) 708, 851
BstYI RGATCY 2 cut(s) 708, 851
Bsu15I ATCGAT 1 cut(s) 1194
BsuI GTATCC 1 cut(s) 289
BsuRI GGCC 3 cut(s) 122, 194, 791
BsuTUI ATCGAT 1 cut(s) 1194
BtgI CCRYGG 1 cut(s) 1074
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 2 cut(s) 895, 1080
Cac8I GCNNGC 2 cut(s) 931, 1145
CaiI CAGNNNCTG 1 cut(s) 421
CfoI GCGC 1 cut(s) 812
Cfr13I GGNCC 4 cut(s) 87, 98, 331, 451
ClaI ATCGAT 1 cut(s) 1194
CseI GACGC 1 cut(s) 956
Csp6I GTAC 1 cut(s) 1132
CspCI CAANNNNNGTGG 2 cut(s) 1056, 1091
CviAII CATG 8 cut(s) 56, 714, 773, 797, 818, 1129, 1135, 1144
CviQI GTAC 1 cut(s) 1132
DdeI CTNAG 7 cut(s) 243, 386, 732, 741, 864, 934, 1158
DpnI GATC 6 cut(s) 264, 414, 504, 710, 853, 1230
DpnII GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
EaeI YGGCCR 1 cut(s) 789
Ecl136II GAGCTC 2 cut(s) 738, 1224
Eco24I GRGCYC 2 cut(s) 740, 1226
Eco47I GGWCC 4 cut(s) 87, 98, 331, 451
Eco53kI GAGCTC 2 cut(s) 738, 1224
Eco88I CYCGRG 1 cut(s) 94
EcoICRI GAGCTC 2 cut(s) 738, 1224
EcoO109I RGGNCCY 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 194, 924
EcoT38I GRGCYC 2 cut(s) 740, 1226
FaeI CATG 8 cut(s) 59, 717, 776, 800, 821, 1132, 1138, 1147
FaqI GGGAC 2 cut(s) 111, 212
FatI CATG 8 cut(s) 55, 713, 772, 796, 817, 1128, 1134, 1143
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 1125
Fnu4HI GCNGC 3 cut(s) 843, 1112, 1148
FokI GGATG 1 cut(s) 394
FriOI GRGCYC 2 cut(s) 740, 1226
Fsp4HI GCNGC 3 cut(s) 843, 1112, 1148
FspBI CTAG 1 cut(s) 1053
GlaI GCGC 1 cut(s) 811
GluI GCNGC 3 cut(s) 843, 1112, 1148
GsaI CCCAGC 2 cut(s) 425, 521
GsuI CTGGAG 2 cut(s) 573, 936
HaeII RGCGCY 1 cut(s) 813
HaeIII GGCC 3 cut(s) 122, 194, 791
HgaI GACGC 1 cut(s) 956
HhaI GCGC 1 cut(s) 812
Hin1II CATG 8 cut(s) 59, 717, 776, 800, 821, 1132, 1138, 1147
Hin6I GCGC 1 cut(s) 810
HinP1I GCGC 1 cut(s) 810
HincII GTYRAC 2 cut(s) 897, 1087
HindII GTYRAC 2 cut(s) 897, 1087
HindIII AAGCTT 4 cut(s) 245, 829, 869, 1178
HphI GGTGA 6 cut(s) 406, 552, 776, 1037, 1055, 1191
Hpy166II GTNNAC 5 cut(s) 897, 1087, 1126, 1155, 1189
Hpy188III TCNNGA 9 cut(s) 96, 253, 266, 407, 460, 835, 977, 1094, 1212
Hpy8I GTNNAC 5 cut(s) 897, 1087, 1126, 1155, 1189
Hpy99I CGWCG 1 cut(s) 841
HpyAV CCTTC 1 cut(s) 462
HpyCH4III ACNGT 4 cut(s) 77, 277, 352, 1084
HpyCH4V TGCA 5 cut(s) 216, 1061, 1067, 1111, 1147
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 744, 797, 818
HpyF3I CTNAG 7 cut(s) 243, 386, 732, 741, 864, 934, 1158
Hsp92II CATG 8 cut(s) 59, 717, 776, 800, 821, 1132, 1138, 1147
HspAI GCGC 1 cut(s) 810
Ksp22I TGATCA 1 cut(s) 1228
Kzo9I GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
LmnI GCTCC 2 cut(s) 955, 1265
Lsp1109I GCAGC 3 cut(s) 829, 1123, 1159
LweI GCATC 3 cut(s) 346, 372, 1048
MaeI CTAG 1 cut(s) 1053
MaeIII GTNAC 2 cut(s) 751, 1078
MalI GATC 6 cut(s) 264, 414, 504, 710, 853, 1230
MboI GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
MboII GAAGA 7 cut(s) 310, 409, 422, 486, 644, 689, 1282
MfeI CAATTG 1 cut(s) 1062
MflI RGATCY 2 cut(s) 708, 851
MhlI GDGCHC 2 cut(s) 740, 1226
MluCI AATT 8 cut(s) 114, 400, 482, 533, 635, 1062, 1216, 1234
MlyI GAGTC 4 cut(s) 737, 869, 947, 1169
MmeI TCCRAC 2 cut(s) 114, 453
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 3 cut(s) 294, 705, 1017
MslI CAYNNNNRTG 1 cut(s) 1133
MspR9I CCNGG 2 cut(s) 196, 926
MunI CAATTG 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 196, 926
MwoI GCNNNNNNNGC 4 cut(s) 61, 744, 797, 818
NdeII GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
NlaIII CATG 8 cut(s) 59, 717, 776, 800, 821, 1132, 1138, 1147
NlaIV GGNNCC 4 cut(s) 99, 438, 1050, 1261
NmuCI GTSAC 1 cut(s) 1078
NspI RCATGY 2 cut(s) 1132, 1147
PaeI GCATGC 1 cut(s) 1147
PciI ACATGT 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 7 cut(s) 5, 310, 557, 617, 965, 1034, 1196
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 688
PkrI GCNGC 3 cut(s) 844, 1113, 1149
PleI GAGTC 4 cut(s) 736, 869, 946, 1168
PpsI GAGTC 4 cut(s) 736, 869, 946, 1168
PpuMI RGGWCCY 1 cut(s) 451
PscI ACATGT 1 cut(s) 1128
PshAI GACNNNNGTC 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 2 cut(s) 740, 1226
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 2 cut(s) 194, 924
PspFI CCCAGC 2 cut(s) 421, 517
PspGI CCWGG 2 cut(s) 194, 924
PspN4I GGNNCC 4 cut(s) 99, 438, 1050, 1261
PspPI GGNCC 4 cut(s) 87, 98, 331, 451
PspPPI RGGWCCY 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 421
PsuI RGATCY 2 cut(s) 708, 851
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 1 cut(s) 1133
RsaNI GTAC 1 cut(s) 1132
RseI CAYNNNNRTG 1 cut(s) 1133
SacI GAGCTC 2 cut(s) 740, 1226
SaqAI TTAA 3 cut(s) 294, 705, 1017
SatI GCNGC 3 cut(s) 843, 1112, 1148
Sau3AI GATC 6 cut(s) 262, 412, 502, 708, 851, 1228
Sau96I GGNCC 4 cut(s) 87, 98, 331, 451
SchI GAGTC 4 cut(s) 737, 869, 947, 1169
ScrFI CCNGG 2 cut(s) 196, 926
SduI GDGCHC 2 cut(s) 740, 1226
SfaNI GCATC 3 cut(s) 346, 372, 1048
SinI GGWCC 4 cut(s) 87, 98, 331, 451
SmiMI CAYNNNNRTG 1 cut(s) 1133
SphI GCATGC 1 cut(s) 1147
Sse9I AATT 8 cut(s) 114, 400, 482, 533, 635, 1062, 1216, 1234
SsiI CCGC 1 cut(s) 303
SspMI CTAG 1 cut(s) 1053
SstI GAGCTC 2 cut(s) 740, 1226
StyD4I CCNGG 2 cut(s) 194, 924
TaaI ACNGT 4 cut(s) 77, 277, 352, 1084
TaqI TCGA 3 cut(s) 321, 1194, 1220
TaqII GACCGA 1 cut(s) 75
TasI AATT 8 cut(s) 114, 400, 482, 533, 635, 1062, 1216, 1234
TatI WGTACW 1 cut(s) 1131
TfiI GAWTC 7 cut(s) 5, 310, 557, 617, 965, 1034, 1196
Tru1I TTAA 3 cut(s) 294, 705, 1017
Tru9I TTAA 3 cut(s) 294, 705, 1017
TscAI CASTG 2 cut(s) 895, 1087
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 3 cut(s) 842, 1111, 1147
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 9 cut(s) 176, 500, 690, 785, 806, 900, 1026, 1151, 1190
TspGWI ACGGA 1 cut(s) 352
TspRI CASTG 2 cut(s) 895, 1087
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 688
VpaK11BI GGWCC 4 cut(s) 87, 98, 331, 451
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 3 cut(s) 482, 1216, 1234
XceI RCATGY 2 cut(s) 1132, 1147
XmiI GTMKAC 1 cut(s) 1125
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.